MS028639 (gene) Bitter gourd (TR) v1

BLAST of MS028639 vs. NCBI nr
Match: XP_022143463.1 (subtilisin-like protease SBT5.3 [Momordica charantia])

HSP 1 Score: 1535.0 bits (3973), Expect = 0.0e+00
Identity = 768/773 (99.35%), Postives = 771/773 (99.74%), Query Frame = 0

Query: 1   MEASNISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLL 60
           MEASNISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLL
Sbjct: 1   MEASNISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLL 60

Query: 61  GSLLGSKKIAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTTHSW 120
           GSLLGSKKIAEESIFYSYTRSFNGFAATLD+NVAARLAKNPQVISVFENRARKLHTTHSW
Sbjct: 61  GSLLGSKKIAEESIFYSYTRSFNGFAATLDENVAARLAKNPQVISVFENRARKLHTTHSW 120

Query: 121 NFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTCQ 180
           NFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPS+WKGTCQ
Sbjct: 121 NFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSRWKGTCQ 180

Query: 181 TDLNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVF 240
           T LNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVF
Sbjct: 181 TYLNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVF 240

Query: 241 GQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVR 300
           GQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVR
Sbjct: 241 GQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVR 300

Query: 301 DFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIAL 360
           DFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIAL
Sbjct: 301 DFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIAL 360

Query: 361 GNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKIL 420
           GNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKIL
Sbjct: 361 GNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKIL 420

Query: 421 VCLAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTRYM 480
           VCL GEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLT+YM
Sbjct: 421 VCLEGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTQYM 480

Query: 481 DSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGN 540
           DSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGN
Sbjct: 481 DSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGN 540

Query: 541 APTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNN 600
           APTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNN
Sbjct: 541 APTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNN 600

Query: 601 LGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYA 660
           LGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYA
Sbjct: 601 LGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYA 660

Query: 661 KSYVCDRSFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTYVARVKVHPEVSISVEP 720
           KSYVCDRSFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTYVARVKVHPEVSISVEP
Sbjct: 661 KSYVCDRSFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTYVARVKVHPEVSISVEP 720

Query: 721 STLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNLEE 774
           STLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNLEE
Sbjct: 721 STLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNLEE 773

BLAST of MS028639 vs. NCBI nr
Match: XP_038904203.1 (uncharacterized protein LOC120090550 [Benincasa hispida])

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 565/775 (72.90%), Postives = 654/775 (84.39%), Query Frame = 0

Query: 1   MEASNISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQ--SSLASTSLLD-SRQLTASHY 60
           MEASN+SPLLL FF  SLLQTS IA  KKSYIVYLGS+  SSL  +SL + SRQ+TA HY
Sbjct: 1   MEASNLSPLLLFFFYFSLLQTSIIA-TKKSYIVYLGSERSSSLDPSSLSEHSRQVTAFHY 60

Query: 61  NLLGSLLGSKKIAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTT 120
           +LLG+LLGS+K+ EE+IFYSYT SFNGFAA LD+  AA LA+NP+VISVFEN+ARKLHTT
Sbjct: 61  DLLGTLLGSQKMTEEAIFYSYTTSFNGFAAKLDEKEAANLARNPKVISVFENKARKLHTT 120

Query: 121 HSWNFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKG 180
            SWNFLG+EN  G+ P NSIWNAAKFGQDII+ANIDTGVWPESKSFSDEG+GP+PSKW+G
Sbjct: 121 RSWNFLGVENDVGI-PSNSIWNAAKFGQDIIIANIDTGVWPESKSFSDEGYGPVPSKWRG 180

Query: 181 TCQTDLNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGA 240
            CQTD NFHCNRKLIGGRYFYKGY AAG  L+ +SLTVRDHDGHGTHTL+TAAGNFV GA
Sbjct: 181 ICQTDSNFHCNRKLIGGRYFYKGYVAAGGTLNATSLTVRDHDGHGTHTLSTAAGNFVTGA 240

Query: 241 NVFGQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGG 300
           NVFG G+GTAKGGAP+ARVAAYKVCWPP    +C DAD LA FEAA+ADGVDVISTSLGG
Sbjct: 241 NVFGHGSGTAKGGAPKARVAAYKVCWPPFLGSQCLDADILAAFEAAVADGVDVISTSLGG 300

Query: 301 SVRDFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASY 360
           +  ++ +DPLAIAAFHA+QQG+V VFSAGN GP P +V+N+APW  TVAA T+DRDFAS 
Sbjct: 301 AADEYFNDPLAIAAFHAVQQGVVVVFSAGNSGPFPMTVTNIAPWINTVAAGTVDRDFASN 360

Query: 361 IALGNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKG 420
           +ALGNK   +G SL+S+APLPKKFYPLIDSV  ++   NVT++ A+FCGEGT DP KVKG
Sbjct: 361 VALGNK---EGVSLSSIAPLPKKFYPLIDSVNAKL--SNVTEFHARFCGEGTLDPMKVKG 420

Query: 421 KILVCLAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLT 480
           KI++C  GEI G EK  +A R GA G+IV ND + GD+I+PELHF+PASD+  +D Q+L 
Sbjct: 421 KIVICQVGEIEGVEKSYQAARAGAVGVIVANDIEKGDEIYPELHFIPASDITNNDAQLLQ 480

Query: 481 RYMDSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYS 540
           +Y++ST  P+ HL KV+T L IKPAP++A FSSRGPN I+  ILKPDITAPGVNILA+YS
Sbjct: 481 KYLNSTTTPMAHLTKVKTLLNIKPAPIIATFSSRGPNPIDPFILKPDITAPGVNILASYS 540

Query: 541 SGNAPTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTR 600
           +GNAPT S  D+RRIPF+VISGTSMSCPHI+GIA LLKSIHP WSPAAIKSAIMTTAKTR
Sbjct: 541 TGNAPTFSSNDQRRIPFNVISGTSMSCPHIAGIAGLLKSIHPDWSPAAIKSAIMTTAKTR 600

Query: 601 DNNLGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRR 660
            NNL +ILD TK+KAT +AYGAG VHPNDA DPGLVYDTT++DYLNFLCARGY A ++++
Sbjct: 601 GNNLQTILDSTKLKATSYAYGAGMVHPNDARDPGLVYDTTIEDYLNFLCARGYNAMEMKK 660

Query: 661 FYAKSYVCDRSFKLTDLNYPSISVSNLTMG-PVTVNRRVKNVGSLGTYVARVKVHPEVSI 720
           FYAK + C +SFK TDLNYPSISV  L +G PVT+NRRVK+VGS GTYVARVKV P V++
Sbjct: 661 FYAKPFNCVKSFKATDLNYPSISVGVLRIGAPVTINRRVKSVGSPGTYVARVKVSPGVAV 720

Query: 721 SVEPSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNL 772
            VEP TLQFSSVGEEKAFKVV Q  GK KR G +FGTLIWSDGKHFVRSPI ++L
Sbjct: 721 LVEPRTLQFSSVGEEKAFKVVLQNTGKVKR-GDVFGTLIWSDGKHFVRSPIAVHL 767

BLAST of MS028639 vs. NCBI nr
Match: XP_016900593.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 535/777 (68.85%), Postives = 638/777 (82.11%), Query Frame = 0

Query: 4   SNISPLLLAF-----FLLSLLQTSTIAIDKKSYIVYLGSQ--SSLASTSLLD-SRQLTAS 63
           SN+   LL F       ++L +++   I+  SYIVYLGS+  SSL  +SL + S+Q+TA 
Sbjct: 75  SNLKMALLLFESASGLNINLNKSTIFPINVPSYIVYLGSEHSSSLDPSSLSEHSQQVTAL 134

Query: 64  HYNLLGSLLGSKKIAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLH 123
           HY+LLGS LGSK +AEE+IFYSYTRSFNGFAA LD   A  LA+NP+VISVFEN+ARKLH
Sbjct: 135 HYDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLH 194

Query: 124 TTHSWNFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKW 183
           TT SWNFLG+EN  G+ P NSIWNAAKFG+D+I+ANIDTGVWPESKSFSDEG+GP+PSKW
Sbjct: 195 TTRSWNFLGVENDIGI-PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKW 254

Query: 184 KGTCQTDLNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVP 243
           +G CQTD NFHCNRKLIGGRYF+KGYEAAG KL+ + LTVRDHDGHGTHTL+TAAGNFV 
Sbjct: 255 RGICQTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVT 314

Query: 244 GANVFGQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSL 303
           GANVFG GNGTAKGGAP+AR  AYKVCWPPL + +CFDAD LA FEAAIADGVDV+STSL
Sbjct: 315 GANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSL 374

Query: 304 GGSVRDFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFA 363
           GG+  ++ +DPLAIAAF A+Q+GI+ VFS GN GP P +V+NV+PW FTVAA+TIDRDF 
Sbjct: 375 GGAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFV 434

Query: 364 SYIALGNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKV 423
           SY+ LGNKK +KG SL+SVA LPKKFYPLI+SV  +    NVT++ AQFCG+GT DP KV
Sbjct: 435 SYVGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKF--SNVTEFHAQFCGKGTLDPMKV 494

Query: 424 KGKILVCLAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQV 483
           KGKI++C  GE  G +KG +A   GAAG+IV ND + GD+I+PELHF+PASD+  +D Q+
Sbjct: 495 KGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQI 554

Query: 484 LTRYMDSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAA 543
           + +Y+ ST+ P+ HL  V+T L +KPAP++A FSSRGPN I+ +ILKPD+TAPGVNILA+
Sbjct: 555 VQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILAS 614

Query: 544 YSSGNAPTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAK 603
           Y +G APT S  DRRRIPF+VISGTSMSCPH++GIA L+KSIHP WSPAAIKSAIMTTAK
Sbjct: 615 YPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAK 674

Query: 604 TRDNNLGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQI 663
           TR NN  +ILD TK+KATP+AYGAG V+PNDA DPGLVYD T+ DYLNFLCARGY A +I
Sbjct: 675 TRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKI 734

Query: 664 RRFYAKSYVCDRSFKLTDLNYPSISVSNLTMG-PVTVNRRVKNVGSLGTYVARVKVHPEV 723
           ++FYAK + C RSFK+TDLNYPSISV  L +G P+T+NRRVKNVGS GTYVARVK  P V
Sbjct: 735 KKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGV 794

Query: 724 SISVEPSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNL 772
           ++++EPSTL F SVGEEK FKVV Q  GK K  G +FGTLIWSDGKHFVRSPI ++L
Sbjct: 795 AVTIEPSTLVFRSVGEEKGFKVVLQNTGKVK-SGDVFGTLIWSDGKHFVRSPIAVHL 847

BLAST of MS028639 vs. NCBI nr
Match: XP_031738719.1 (uncharacterized protein LOC101212014 [Cucumis sativus])

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 528/754 (70.03%), Postives = 622/754 (82.49%), Query Frame = 0

Query: 22   STIAIDKKSYIVYLGSQ--SSLASTSLLD-SRQLTASHYNLLGSLLGSKKIAEESIFYSY 81
            S I ++  SYIVYLGS+  S+L  +SL + SRQ+TA HY+LLGSL GSK +AEE+IFYSY
Sbjct: 788  SPIVVNLGSYIVYLGSEHSSALDPSSLSEHSRQVTALHYDLLGSLFGSKTMAEEAIFYSY 847

Query: 82   TRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTTHSWNFLGMENAQGVPPPNSIW 141
            TRSFNGFAA LD   A  LA+NP+VISVFEN+ARKLHTT SWNFLG+EN  G+ P NSIW
Sbjct: 848  TRSFNGFAAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI-PSNSIW 907

Query: 142  NAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTCQTDLNFHCNRKLIGGRYFY 201
            N AKFG+D+IVANIDTGVWPESKSFSDEG+GP+PSKW+G CQTD  FHCNRKLIGGRYF+
Sbjct: 908  NTAKFGEDVIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLIGGRYFH 967

Query: 202  KGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVFGQGNGTAKGGAPRARVAA 261
            KGYEAAG KL+ + LTVRDHDGHGTHTL+TAAGNFV GANVFG GNGTAKGGAP+AR  A
Sbjct: 968  KGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVA 1027

Query: 262  YKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVRDFADDPLAIAAFHAMQQG 321
            YK CWPPL + +CFDAD LA FEAAIADGVDV+STSLGG+  ++ +DPLAIAAF A+Q+G
Sbjct: 1028 YKACWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRG 1087

Query: 322  IVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIALGNKKRIKGASLASVAPLP 381
            I+ VFS GN GP P +++N++PW FTVAASTIDR+FASY+ LGNKK IKG SL+SV  LP
Sbjct: 1088 ILVVFSGGNSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLSSVPSLP 1147

Query: 382  KKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKILVCLAGEIVGFEKGIEAMR 441
            KKF+PLI+SV  +   RNVT++ AQFCG+GT DP KVKGKI++C  GE  G +KG +A R
Sbjct: 1148 KKFFPLINSVDAKF--RNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGFQASR 1207

Query: 442  VGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTRYMDSTRNPVGHLMKVRTELG 501
             GA G+I+ ND + GD+IFPELHF+PASD+  +D Q++  Y+ STR P+ HL  V+T L 
Sbjct: 1208 AGAVGVIIANDLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVKTLLS 1267

Query: 502  IKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGNAPTTSRYDRRRIPFSVIS 561
            +KPAP +A FS+RGPN I+ +ILKPD+TAPGVNILA+Y +G APT S  DRRRIPF+VIS
Sbjct: 1268 VKPAPTIATFSARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPFNVIS 1327

Query: 562  GTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNNLGSILDLTKVKATPFAYG 621
            GTSMSCPH++GIA L+KSIHP WSPAAIKSAIMTTAKTR NN  +ILD TK+KATP+AYG
Sbjct: 1328 GTSMSCPHVAGIAGLIKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYG 1387

Query: 622  AGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYAKSYVCDRSFKLTDLNYPS 681
            AG V+PNDA DPGLVYD TV DYLNFLCARGY A QI++FYAK + C RSFK+TDLNYPS
Sbjct: 1388 AGQVNPNDAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDLNYPS 1447

Query: 682  ISVSNLTMG-PVTVNRRVKNVGSLGTYVARVKVHPEVSISVEPSTLQFSSVGEEKAFKVV 741
            ISV  L +G P+T+NRRVKNVGS GTYVARVK  P V++S+EPSTL FS VGEEK FKVV
Sbjct: 1448 ISVGELKIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKGFKVV 1507

Query: 742  FQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNL 772
             Q  GK K    +FGTLIWSDGKHFVRS I ++L
Sbjct: 1508 LQNTGKVKNGSDVFGTLIWSDGKHFVRSSIAVHL 1538

BLAST of MS028639 vs. NCBI nr
Match: KAE8650095.1 (hypothetical protein Csa_011146 [Cucumis sativus])

HSP 1 Score: 1059.7 bits (2739), Expect = 1.2e-305
Identity = 521/744 (70.03%), Postives = 612/744 (82.26%), Query Frame = 0

Query: 29  KSYIVYLGSQ--SSLASTSLLD-SRQLTASHYNLLGSLLGSKKIAEESIFYSYTRSFNGF 88
           +SYIVYLGS+  S+L  +SL + SRQ+TA HY+LLGSL GSK +AEE+IFYSYTRSFNGF
Sbjct: 2   QSYIVYLGSEHSSALDPSSLSEHSRQVTALHYDLLGSLFGSKTMAEEAIFYSYTRSFNGF 61

Query: 89  AATLDQNVAARLAKNPQVISVFENRARKLHTTHSWNFLGMENAQGVPPPNSIWNAAKFGQ 148
           AA LD   A  LA+NP+VISVFEN+ARKLHTT SWNFLG+EN  G+ P NSIWN AKFG+
Sbjct: 62  AAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI-PSNSIWNTAKFGE 121

Query: 149 DIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTCQTDLNFHCNRKLIGGRYFYKGYEAAG 208
           D+IVANIDTGVWPESKSFSDEG+GP+PSKW+G CQTD  FHCNRKLIGGRYF+KGYEAAG
Sbjct: 122 DVIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLIGGRYFHKGYEAAG 181

Query: 209 AKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVFGQGNGTAKGGAPRARVAAYKVCWPP 268
            KL+ + LTVRDHDGHGTHTL+TAAGNFV GANVFG GNGTAKGGAP+AR  AYK CWPP
Sbjct: 182 GKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKACWPP 241

Query: 269 LPEGECFDADTLAGFEAAIADGVDVISTSLGGSVRDFADDPLAIAAFHAMQQGIVTVFSA 328
           L + +CFDAD LA FEAAIADGVDV+STSLGG+  ++ +DPLAIAAF A+Q+GI+ VFS 
Sbjct: 242 LFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSG 301

Query: 329 GNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIALGNKKRIKGASLASVAPLPKKFYPLI 388
           GN GP P +++N++PW FTVAASTIDR+FASY+ LGNKK IKG SL+SV  LPKKF+PLI
Sbjct: 302 GNSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLSSVPSLPKKFFPLI 361

Query: 389 DSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKILVCLAGEIVGFEKGIEAMRVGAAGLI 448
           +SV  +   RNVT++ AQFCG+GT DP KVKGKI++C  GE  G +KG +A R GA G+I
Sbjct: 362 NSVDAKF--RNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGFQASRAGAVGVI 421

Query: 449 VVNDKDSGDDIFPELHFLPASDVKYSDRQVLTRYMDSTRNPVGHLMKVRTELGIKPAPVM 508
           + ND + GD+IFPELHF+PASD+  +D Q++  Y+ STR P+ HL  V+T L +KPAP +
Sbjct: 422 IANDLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVKTLLSVKPAPTI 481

Query: 509 ALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGNAPTTSRYDRRRIPFSVISGTSMSCP 568
           A FS+RGPN I+ +ILKPD+TAPGVNILA+Y +G APT S  DRRRIPF+VISGTSMSCP
Sbjct: 482 ATFSARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPFNVISGTSMSCP 541

Query: 569 HISGIAALLKSIHPQWSPAAIKSAIMTT------AKTRDNNLGSILDLTKVKATPFAYGA 628
           H++GIA L+KSIHP WSPAAIKSAIMTT      AKTR NN  +ILD TK+KATP+AYGA
Sbjct: 542 HVAGIAGLIKSIHPNWSPAAIKSAIMTTVTYSFAAKTRGNNNQTILDSTKLKATPYAYGA 601

Query: 629 GHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYAKSYVCDRSFKLTDLNYPSI 688
           G V+PNDA DPGLVYD TV DYLNFLCARGY A QI++FYAK + C RSFK+TDLNYPSI
Sbjct: 602 GQVNPNDAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDLNYPSI 661

Query: 689 SVSNLTMG-PVTVNRRVKNVGSLGTYVARVKVHPEVSISVEPSTLQFSSVGEEKAFKVVF 748
           SV  L +G P+T+NRRVKNVGS GTYVARVK  P V++S+EPSTL FS VGEEK FKVV 
Sbjct: 662 SVGELKIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKGFKVVL 721

Query: 749 QYKGKGKREGSMFGTLIWSDGKHF 763
           Q  GK K    +FGTLIWSDGKHF
Sbjct: 722 QNTGKVKNGSDVFGTLIWSDGKHF 742

BLAST of MS028639 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 1.3e-233
Identity = 415/771 (53.83%), Postives = 537/771 (69.65%), Query Frame = 0

Query: 5   NISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLLGSLL 64
           N S LLL   +    +    + D  SY+VY G+ S +   +     ++  +HY+ LGS  
Sbjct: 6   NFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFT 65

Query: 65  GSKKIAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTTHSWNFLG 124
           GS++ A ++IFYSYT+  NGFAA LD ++A  ++K+P+V+SVF N+A KLHTT SW+FLG
Sbjct: 66  GSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLG 125

Query: 125 MENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTCQT--D 184
           +E+   V P +SIW  A+FG+D I+AN+DTGVWPESKSF DEG GPIPS+WKG CQ   D
Sbjct: 126 LEHNSYV-PSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKD 185

Query: 185 LNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVFGQ 244
             FHCNRKLIG RYF KGY AA   L+ S  + RD DGHG+HTL+TAAG+FVPG ++FGQ
Sbjct: 186 ATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQ 245

Query: 245 GNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVRDF 304
           GNGTAKGG+PRARVAAYKVCWPP+   EC+DAD LA F+AAI DG DVIS SLGG    F
Sbjct: 246 GNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSF 305

Query: 305 ADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIALGN 364
            +D +AI +FHA ++ IV V SAGN GP   +VSNVAPW+ TV AST+DR+FAS + LGN
Sbjct: 306 FNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGN 365

Query: 365 KKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKILVC 424
            K  KG SL+S A    KFYP++ SV  +  ++N +   AQ C  G+ DP K KGKILVC
Sbjct: 366 GKHYKGQSLSSTALPHAKFYPIMASVNAK--AKNASALDAQLCKLGSLDPIKTKGKILVC 425

Query: 425 LAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTRYMDS 484
           L G+    EKG      G  G+++ N   +G+D+  + H LPA+ +   D   ++RY+  
Sbjct: 426 LRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQ 485

Query: 485 TRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGNAP 544
           T+ P+ H+   RT+LG+KPAPVMA FSS+GP+ +   ILKPDITAPGV+++AAY+   +P
Sbjct: 486 TKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSP 545

Query: 545 TTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNNLG 604
           T  ++D RR+ F+ ISGTSMSCPHISGIA LLK+ +P WSPAAI+SAIMTTA   D+  G
Sbjct: 546 TNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPG 605

Query: 605 SILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYAKS 664
            I + T +KATPF++GAGHV PN A++PGLVYD  +KDYLNFLC+ GY A QI  F   +
Sbjct: 606 PIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNN 665

Query: 665 YVCDR-SFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTYVARVKVHPEVSISVEPS 724
           + C      L +LNYPSI+V NLT   VTV+R VKNVG    Y  +V     V ++V+P+
Sbjct: 666 FTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPT 725

Query: 725 TLQFSSVGEEKAFKVVFQYKGKGK-REGSMFGTLIWSDGKHFVRSPIVMNL 772
           +L F+ VGE+K FKV+   K KG   +G +FG L+WSD KH VRSPIV+ L
Sbjct: 726 SLNFTKVGEQKTFKVIL-VKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of MS028639 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 1.9e-229
Identity = 416/774 (53.75%), Postives = 526/774 (67.96%), Query Frame = 0

Query: 1   MEASNISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLL 60
           M   ++S LLL   L++L  +   A+ KKSYIVYLGS + L   S      +  SH   L
Sbjct: 16  MSLQSLSSLLL---LVTLFFSPAFAL-KKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFL 75

Query: 61  GSLLGSKKIAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTTHSW 120
            S +GS + A+E+IFYSY R  NGFAA LD+N AA +AK+P V+SVF N+ RKLHTTHSW
Sbjct: 76  ASFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSW 135

Query: 121 NFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTCQ 180
           NF+ +    GV   +S+WN A +G+D I+AN+DTGVWPESKSFSDEG+G +P++WKG C 
Sbjct: 136 NFMLLAK-NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH 195

Query: 181 TDLNFHCNRKLIGGRYFYKGYEA-AGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANV 240
            D+   CNRKLIG RYF KGY A  G   + S  T RDHDGHG+HTL+TAAGNFVPGANV
Sbjct: 196 KDV--PCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANV 255

Query: 241 FGQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSV 300
           FG GNGTA GG+P+ARVAAYKVCWPP+   ECFDAD LA  EAAI DGVDV+S S+GG  
Sbjct: 256 FGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDA 315

Query: 301 RDFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIA 360
            D+  D +AI +FHA++ G+  V SAGN GP  G+VSNVAPW  TV AS++DR+F +++ 
Sbjct: 316 GDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVE 375

Query: 361 LGNKKRIKGASLASVAPLP-KKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGK 420
           L N +  KG SL+   PLP +K Y LI +    + + NVTD  A  C +G+ DP KVKGK
Sbjct: 376 LKNGQSFKGTSLSK--PLPEEKMYSLISAADANVANGNVTD--ALLCKKGSLDPKKVKGK 435

Query: 421 ILVCLAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTR 480
           ILVCL G+    +KG++A   GAAG+++ NDK SG++I  + H LPAS + Y D + L  
Sbjct: 436 ILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFS 495

Query: 481 YMDSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSS 540
           Y+ ST++P G++      L  KPAP MA FSSRGPN I   ILKPDITAPGVNI+AA++ 
Sbjct: 496 YLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTE 555

Query: 541 GNAPTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRD 600
              PT    D RR PF+  SGTSMSCPHISG+  LLK++HP WSPAAI+SAIMTT++TR+
Sbjct: 556 ATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRN 615

Query: 601 NNLGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRF 660
           N    ++D +  KA PF+YG+GHV PN A  PGLVYD T  DYL+FLCA GY    ++ F
Sbjct: 616 NRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLF 675

Query: 661 YA-KSYVCDRSFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTYVARVKVHPEVSIS 720
                Y C +   L D NYPSI+V NLT G +TV R++KNVG   TY AR +    V +S
Sbjct: 676 AEDPQYTCRQGANLLDFNYPSITVPNLT-GSITVTRKLKNVGPPATYNARFREPLGVRVS 735

Query: 721 VEPSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNL 772
           VEP  L F+  GE K F++  +        G +FG L W+D  H+VRSPIV+ L
Sbjct: 736 VEPKQLTFNKTGEVKIFQMTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of MS028639 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 695.3 bits (1793), Expect = 7.9e-199
Identity = 388/785 (49.43%), Postives = 494/785 (62.93%), Query Frame = 0

Query: 9   LLLAFFLLSLLQTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLLGSLLGSKK 68
           L+L+ F L     + +   KK YIVY+G+ S   S +  D    T SHY+LLGS+ GS++
Sbjct: 10  LILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSRE 69

Query: 69  IAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTTHSWNFLGMENA 128
            A+E+I YSY R  NGFAA L++  AA +AKNP V+SVF ++  KLHTT SW FLG+   
Sbjct: 70  KAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRR 129

Query: 129 QGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWK-GTCQTD----- 188
                 NS W   +FG++ I+ NIDTGVWPES+SFSD+G+G +PSKW+ G CQ +     
Sbjct: 130 ----GQNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGS 189

Query: 189 LNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVFGQ 248
           +   CNRKLIG RY+ K +EA   +L P   T RD  GHGTHTL+TA GNFVPGA VF  
Sbjct: 190 MKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAV 249

Query: 249 GNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVRDF 308
           GNGTAKGG+PRARVAAYKVCW       C+ AD LA  + AI DGVDVI+ S G S    
Sbjct: 250 GNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVT 309

Query: 309 AD----DPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYI 368
           A+    D ++I AFHA+ + I+ V SAGNDGP PG+V+NVAPW FT+AAST+DRDF+S +
Sbjct: 310 AEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNL 369

Query: 369 ALGNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGK 428
            + N + I+GASL    P P + + LI S   ++   N T   AQ C  GT D  KV GK
Sbjct: 370 TI-NNQLIEGASLFVNLP-PNQAFSLILSTDAKL--ANATFRDAQLCRRGTLDRTKVNGK 429

Query: 429 ILVCL-AGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFL-----PASDVKYSD 488
           I++C   G+I    +G+EA+  GA G+I+ N   +G  +  E H       P    K   
Sbjct: 430 IVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRP 489

Query: 489 RQVLTRYMDSTRNPVG-----HLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITA 548
             V T  +    +P+       + + RT  G KPAPVMA FSSRGPN I+ SILKPD+TA
Sbjct: 490 HGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTA 549

Query: 549 PGVNILAAYSSGNAPTTSRYDRRR-IPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAI 608
           PGVNILAAYS   + ++   D RR   F+V+ GTSMSCPH SGIA LLK+ HP WSPAAI
Sbjct: 550 PGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAI 609

Query: 609 KSAIMTTAKTRDNNLGSILD-LTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFL 668
           KSAIMTTA T DN    I D   K  A  FAYG+GHV P+ A++PGLVYD ++ DYLNFL
Sbjct: 610 KSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFL 669

Query: 669 CARGYTAKQIRRF-YAKSYVCDRSFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTY 728
           CA GY  + I    + ++++C  S  + DLNYPSI++ NL + PVT+ R V NVG   TY
Sbjct: 670 CASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTY 729

Query: 729 VARVKVHPEVSISVEPSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVR 770
               +     SI+V P +L F+ +GE K FKV+ Q      R    FG L W+DGKH VR
Sbjct: 730 TVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVR 786

BLAST of MS028639 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 5.2e-174
Identity = 347/771 (45.01%), Postives = 462/771 (59.92%), Query Frame = 0

Query: 12  AFFLLSLL---QTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLLGSLLGSKK 71
           AFFLL  L     S+ + D+ +YIV++      +S  L         H N   S L S  
Sbjct: 10  AFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDL---------HSNWYDSSLRSIS 69

Query: 72  IAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTTHSWNFLGMENA 131
            + E + Y+Y  + +GF+  L Q  A  L   P VISV      +LHTT +  FLG++  
Sbjct: 70  DSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH 129

Query: 132 QGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTCQTDLNFH-- 191
                   ++  A    D++V  +DTGVWPESKS+SDEGFGPIPS WKG C+   NF   
Sbjct: 130 TA-----DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTAS 189

Query: 192 -CNRKLIGGRYFYKGYEAAGAKL--SPSSLTVRDHDGHGTHTLATAAGNFVPGANVFGQG 251
            CNRKLIG R+F +GYE+    +  S  S + RD DGHGTHT +TAAG+ V GA++ G  
Sbjct: 190 LCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 249

Query: 252 NGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVRDFA 311
           +GTA+G APRARVA YKVCW     G CF +D LA  + AIAD V+V+S SLGG + D+ 
Sbjct: 250 SGTARGMAPRARVAVYKVCW----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYY 309

Query: 312 DDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIALGNK 371
            D +AI AF AM++GI+   SAGN GP+  S+SNVAPW  TV A T+DRDF +   LGN 
Sbjct: 310 RDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNG 369

Query: 372 KRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKILVCL 431
           K   G SL     LP K  P I   AG   + N T+     C  GT  P KVKGKI++C 
Sbjct: 370 KNFTGVSLFKGEALPDKLLPFI--YAGN--ASNATN--GNLCMTGTLIPEKVKGKIVMCD 429

Query: 432 AGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTRYMDST 491
            G     +KG      G  G+I+ N   +G+++  + H LPA+ V      ++  Y+ + 
Sbjct: 430 RGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTD 489

Query: 492 RNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGNAPT 551
            NP   +  + T +G+KP+PV+A FSSRGPN+I  +ILKPD+ APGVNILAA++    PT
Sbjct: 490 PNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPT 549

Query: 552 TSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNNLGS 611
               D RR+ F++ISGTSMSCPH+SG+AALLKS+HP+WSPAAI+SA+MTTA     +   
Sbjct: 550 GLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKP 609

Query: 612 ILDLTKVK-ATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYAKS 671
           +LD+   K +TPF +GAGHV P  A +PGL+YD T +DYL FLCA  YT+ QIR    ++
Sbjct: 610 LLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRN 669

Query: 672 YVCD--RSFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTYVARVKVHPE-VSISVE 731
           Y CD  +S+ + DLNYPS +V+   +G     R V +VG  GTY  +V      V ISVE
Sbjct: 670 YTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVE 729

Query: 732 PSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMN 771
           P+ L F    E+K++ V F          + FG++ WSDGKH V SP+ ++
Sbjct: 730 PAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of MS028639 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.1e-155
Identity = 320/789 (40.56%), Postives = 460/789 (58.30%), Query Frame = 0

Query: 8   PLLLAFFLLSLL----QTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLLGSL 67
           P L     ++L+    +T+T    KK+Y++++    S       +  Q  +S  N   S+
Sbjct: 10  PFLFIILSINLIFLQAETTTQISTKKTYVIHM--DKSAMPLPYTNHLQWYSSKIN---SV 69

Query: 68  LGSKKIAEES----IFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTTHS 127
              K   EE     I Y+Y  +F+G AA L Q  A RL +   V++V      +LHTT S
Sbjct: 70  TQHKSQEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRS 129

Query: 128 WNFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTC 187
             FLG+E  +       +W       D++V  +DTG+WPES+SF+D G  P+P+ W+G C
Sbjct: 130 PTFLGLERQES----ERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGAC 189

Query: 188 QTDLNF---HCNRKLIGGRYFYKGYEAAGAKLSP--SSLTVRDHDGHGTHTLATAAGNFV 247
           +T   F   +CNRK++G R FY+GYEAA  K+       + RD DGHGTHT AT AG+ V
Sbjct: 190 ETGKRFLKRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPV 249

Query: 248 PGANVFGQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTS 307
            GAN+FG   GTA+G A +ARVAAYKVCW     G CF +D L+  + A+ADGV V+S S
Sbjct: 250 KGANLFGFAYGTARGMAQKARVAAYKVCW----VGGCFSSDILSAVDQAVADGVQVLSIS 309

Query: 308 LGGSVRDFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDF 367
           LGG V  ++ D L+IA F AM+ G+    SAGN GP+P S++NV+PW  TV AST+DRDF
Sbjct: 310 LGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDF 369

Query: 368 ASYIALGNKKRIKGASL-ASVAPLPK-KFYPLIDSVAGRIISRNVTD-YRAQFCGEGTQD 427
            + + +G  +  KG SL      LPK K YPL+       + RN +      FC +G  D
Sbjct: 370 PATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLV------YLGRNASSPDPTSFCLDGALD 429

Query: 428 PAKVKGKILVCLAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYS 487
              V GKI++C  G     +KG    R G  G+++ N   +G+++  + H LPA  V   
Sbjct: 430 RRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEK 489

Query: 488 DRQVLTRYMDSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVN 547
           + +++ +Y  +++     L  + T +GIKP+PV+A FSSRGPN +   ILKPD+ APGVN
Sbjct: 490 EGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVN 549

Query: 548 ILAAYSSGNAPTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIM 607
           ILAA++   AP++   D RR+ F+++SGTSMSCPH+SG+AAL+KS HP WSPAAIKSA+M
Sbjct: 550 ILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALM 609

Query: 608 TTAKTRDNNLGSILDLT-KVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGY 667
           TTA   DN    + D +    ++P+ +GAGH+ P  A DPGLVYD   ++Y  FLC +  
Sbjct: 610 TTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDL 669

Query: 668 TAKQIRRFYAKSYVCDRSFKLT------DLNYPSISV---SNLTMGPVTVNRRVKNVG-S 727
           +  Q++ F   S   +R+ K T      +LNYP+IS     N  +  +T+ R V NVG  
Sbjct: 670 SPSQLKVFTKHS---NRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPH 729

Query: 728 LGTYVARVKVHPEVSISVEPSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGK 770
           + +Y   V      S++V+P TL F+S  ++ ++ V F+ + + KR    FG L+W    
Sbjct: 730 ISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR--PEFGGLVWKSTT 774

BLAST of MS028639 vs. ExPASy TrEMBL
Match: A0A6J1CQS4 (subtilisin-like protease SBT5.3 OS=Momordica charantia OX=3673 GN=LOC111013337 PE=3 SV=1)

HSP 1 Score: 1535.0 bits (3973), Expect = 0.0e+00
Identity = 768/773 (99.35%), Postives = 771/773 (99.74%), Query Frame = 0

Query: 1   MEASNISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLL 60
           MEASNISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLL
Sbjct: 1   MEASNISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLL 60

Query: 61  GSLLGSKKIAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTTHSW 120
           GSLLGSKKIAEESIFYSYTRSFNGFAATLD+NVAARLAKNPQVISVFENRARKLHTTHSW
Sbjct: 61  GSLLGSKKIAEESIFYSYTRSFNGFAATLDENVAARLAKNPQVISVFENRARKLHTTHSW 120

Query: 121 NFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTCQ 180
           NFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPS+WKGTCQ
Sbjct: 121 NFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSRWKGTCQ 180

Query: 181 TDLNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVF 240
           T LNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVF
Sbjct: 181 TYLNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVF 240

Query: 241 GQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVR 300
           GQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVR
Sbjct: 241 GQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVR 300

Query: 301 DFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIAL 360
           DFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIAL
Sbjct: 301 DFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIAL 360

Query: 361 GNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKIL 420
           GNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKIL
Sbjct: 361 GNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKIL 420

Query: 421 VCLAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTRYM 480
           VCL GEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLT+YM
Sbjct: 421 VCLEGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTQYM 480

Query: 481 DSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGN 540
           DSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGN
Sbjct: 481 DSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGN 540

Query: 541 APTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNN 600
           APTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNN
Sbjct: 541 APTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNN 600

Query: 601 LGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYA 660
           LGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYA
Sbjct: 601 LGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYA 660

Query: 661 KSYVCDRSFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTYVARVKVHPEVSISVEP 720
           KSYVCDRSFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTYVARVKVHPEVSISVEP
Sbjct: 661 KSYVCDRSFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTYVARVKVHPEVSISVEP 720

Query: 721 STLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNLEE 774
           STLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNLEE
Sbjct: 721 STLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNLEE 773

BLAST of MS028639 vs. ExPASy TrEMBL
Match: A0A1S4DX90 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491001 PE=3 SV=1)

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 535/777 (68.85%), Postives = 638/777 (82.11%), Query Frame = 0

Query: 4   SNISPLLLAF-----FLLSLLQTSTIAIDKKSYIVYLGSQ--SSLASTSLLD-SRQLTAS 63
           SN+   LL F       ++L +++   I+  SYIVYLGS+  SSL  +SL + S+Q+TA 
Sbjct: 75  SNLKMALLLFESASGLNINLNKSTIFPINVPSYIVYLGSEHSSSLDPSSLSEHSQQVTAL 134

Query: 64  HYNLLGSLLGSKKIAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLH 123
           HY+LLGS LGSK +AEE+IFYSYTRSFNGFAA LD   A  LA+NP+VISVFEN+ARKLH
Sbjct: 135 HYDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLH 194

Query: 124 TTHSWNFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKW 183
           TT SWNFLG+EN  G+ P NSIWNAAKFG+D+I+ANIDTGVWPESKSFSDEG+GP+PSKW
Sbjct: 195 TTRSWNFLGVENDIGI-PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKW 254

Query: 184 KGTCQTDLNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVP 243
           +G CQTD NFHCNRKLIGGRYF+KGYEAAG KL+ + LTVRDHDGHGTHTL+TAAGNFV 
Sbjct: 255 RGICQTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVT 314

Query: 244 GANVFGQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSL 303
           GANVFG GNGTAKGGAP+AR  AYKVCWPPL + +CFDAD LA FEAAIADGVDV+STSL
Sbjct: 315 GANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSL 374

Query: 304 GGSVRDFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFA 363
           GG+  ++ +DPLAIAAF A+Q+GI+ VFS GN GP P +V+NV+PW FTVAA+TIDRDF 
Sbjct: 375 GGAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFV 434

Query: 364 SYIALGNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKV 423
           SY+ LGNKK +KG SL+SVA LPKKFYPLI+SV  +    NVT++ AQFCG+GT DP KV
Sbjct: 435 SYVGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKF--SNVTEFHAQFCGKGTLDPMKV 494

Query: 424 KGKILVCLAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQV 483
           KGKI++C  GE  G +KG +A   GAAG+IV ND + GD+I+PELHF+PASD+  +D Q+
Sbjct: 495 KGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQI 554

Query: 484 LTRYMDSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAA 543
           + +Y+ ST+ P+ HL  V+T L +KPAP++A FSSRGPN I+ +ILKPD+TAPGVNILA+
Sbjct: 555 VQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILAS 614

Query: 544 YSSGNAPTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAK 603
           Y +G APT S  DRRRIPF+VISGTSMSCPH++GIA L+KSIHP WSPAAIKSAIMTTAK
Sbjct: 615 YPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAK 674

Query: 604 TRDNNLGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQI 663
           TR NN  +ILD TK+KATP+AYGAG V+PNDA DPGLVYD T+ DYLNFLCARGY A +I
Sbjct: 675 TRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKI 734

Query: 664 RRFYAKSYVCDRSFKLTDLNYPSISVSNLTMG-PVTVNRRVKNVGSLGTYVARVKVHPEV 723
           ++FYAK + C RSFK+TDLNYPSISV  L +G P+T+NRRVKNVGS GTYVARVK  P V
Sbjct: 735 KKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGV 794

Query: 724 SISVEPSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNL 772
           ++++EPSTL F SVGEEK FKVV Q  GK K  G +FGTLIWSDGKHFVRSPI ++L
Sbjct: 795 AVTIEPSTLVFRSVGEEKGFKVVLQNTGKVK-SGDVFGTLIWSDGKHFVRSPIAVHL 847

BLAST of MS028639 vs. ExPASy TrEMBL
Match: A0A0A0L241 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045090 PE=3 SV=1)

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 526/747 (70.41%), Postives = 619/747 (82.86%), Query Frame = 0

Query: 29  KSYIVYLGSQ--SSLASTSLLD-SRQLTASHYNLLGSLLGSKKIAEESIFYSYTRSFNGF 88
           +SYIVYLGS+  S+L  +SL + SRQ+TA HY+LLGSL GSK +AEE+IFYSYTRSFNGF
Sbjct: 2   QSYIVYLGSEHSSALDPSSLSEHSRQVTALHYDLLGSLFGSKTMAEEAIFYSYTRSFNGF 61

Query: 89  AATLDQNVAARLAKNPQVISVFENRARKLHTTHSWNFLGMENAQGVPPPNSIWNAAKFGQ 148
           AA LD   A  LA+NP+VISVFEN+ARKLHTT SWNFLG+EN  G+ P NSIWN AKFG+
Sbjct: 62  AAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI-PSNSIWNTAKFGE 121

Query: 149 DIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTCQTDLNFHCNRKLIGGRYFYKGYEAAG 208
           D+IVANIDTGVWPESKSFSDEG+GP+PSKW+G CQTD  FHCNRKLIGGRYF+KGYEAAG
Sbjct: 122 DVIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLIGGRYFHKGYEAAG 181

Query: 209 AKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVFGQGNGTAKGGAPRARVAAYKVCWPP 268
            KL+ + LTVRDHDGHGTHTL+TAAGNFV GANVFG GNGTAKGGAP+AR  AYK CWPP
Sbjct: 182 GKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKACWPP 241

Query: 269 LPEGECFDADTLAGFEAAIADGVDVISTSLGGSVRDFADDPLAIAAFHAMQQGIVTVFSA 328
           L + +CFDAD LA FEAAIADGVDV+STSLGG+  ++ +DPLAIAAF A+Q+GI+ VFS 
Sbjct: 242 LFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSG 301

Query: 329 GNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIALGNKKRIKGASLASVAPLPKKFYPLI 388
           GN GP P +++N++PW FTVAASTIDR+FASY+ LGNKK IKG SL+SV  LPKKF+PLI
Sbjct: 302 GNSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLSSVPSLPKKFFPLI 361

Query: 389 DSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKILVCLAGEIVGFEKGIEAMRVGAAGLI 448
           +SV  +   RNVT++ AQFCG+GT DP KVKGKI++C  GE  G +KG +A R GA G+I
Sbjct: 362 NSVDAKF--RNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGFQASRAGAVGVI 421

Query: 449 VVNDKDSGDDIFPELHFLPASDVKYSDRQVLTRYMDSTRNPVGHLMKVRTELGIKPAPVM 508
           + ND + GD+IFPELHF+PASD+  +D Q++  Y+ STR P+ HL  V+T L +KPAP +
Sbjct: 422 IANDLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVKTLLSVKPAPTI 481

Query: 509 ALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGNAPTTSRYDRRRIPFSVISGTSMSCP 568
           A FS+RGPN I+ +ILKPD+TAPGVNILA+Y +G APT S  DRRRIPF+VISGTSMSCP
Sbjct: 482 ATFSARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPFNVISGTSMSCP 541

Query: 569 HISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNNLGSILDLTKVKATPFAYGAGHVHPN 628
           H++GIA L+KSIHP WSPAAIKSAIMTTAKTR NN  +ILD TK+KATP+AYGAG V+PN
Sbjct: 542 HVAGIAGLIKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVNPN 601

Query: 629 DAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYAKSYVCDRSFKLTDLNYPSISVSNLT 688
           DA DPGLVYD TV DYLNFLCARGY A QI++FYAK + C RSFK+TDLNYPSISV  L 
Sbjct: 602 DAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDLNYPSISVGELK 661

Query: 689 MG-PVTVNRRVKNVGSLGTYVARVKVHPEVSISVEPSTLQFSSVGEEKAFKVVFQYKGKG 748
           +G P+T+NRRVKNVGS GTYVARVK  P V++S+EPSTL FS VGEEK FKVV Q  GK 
Sbjct: 662 IGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKGFKVVLQNTGKV 721

Query: 749 KREGSMFGTLIWSDGKHFVRSPIVMNL 772
           K    +FGTLIWSDGKHFVRS I ++L
Sbjct: 722 KNGSDVFGTLIWSDGKHFVRSSIAVHL 745

BLAST of MS028639 vs. ExPASy TrEMBL
Match: A0A5D3D763 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00150 PE=3 SV=1)

HSP 1 Score: 1059.3 bits (2738), Expect = 7.7e-306
Identity = 533/779 (68.42%), Postives = 630/779 (80.87%), Query Frame = 0

Query: 1   MEASNISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQ--SSLASTSLLD-SRQLTASHY 60
           MEASN+SPLLL FF  SLLQTSTIA  KKSYIVYLGS+  SSL  +SL + S+Q+TA HY
Sbjct: 1   MEASNLSPLLLFFFYFSLLQTSTIA-TKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHY 60

Query: 61  NLLGSLLGSKKIAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTT 120
           +LLGS LGSK +AEE+IFYSYTRSFNGFAA LD   A  LA+NP+VISVFEN+ARKLHTT
Sbjct: 61  DLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTT 120

Query: 121 HSWNFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKG 180
            SWNFLG+EN  G+ P NSIWNAAKFG+D+I+ANIDTGVWPESKSFSDEG+GP+PSKW+G
Sbjct: 121 RSWNFLGVENDIGI-PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRG 180

Query: 181 TCQTDLNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGA 240
            CQTD NF CNRKLIGGRYF+KGYEAAG KL+ + LTVRDHDGHGTHTL+TAAGNFV GA
Sbjct: 181 ICQTDSNFLCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGA 240

Query: 241 NVFGQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGG 300
           NVFG GNGTAKGGAP+AR  AYKVCWPPL + +CFDAD LA FEAAIADGVDV+STSLGG
Sbjct: 241 NVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGG 300

Query: 301 SVRDFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASY 360
           +  ++ +DPLAIAAF A+Q+GI+ VFS GN GP P +V+NV+PW FTVAA+TIDRDF SY
Sbjct: 301 AADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSY 360

Query: 361 IALGNKKRIKGASLASVAPL-PKKFYP---LIDSVAGRIISRNVTDYRAQFCGEGTQDPA 420
           + LGNKK IK      +  L   +F P       +  +    +  +   QFCG+GT DP 
Sbjct: 361 VGLGNKKHIKVHVYTHLLGLGASRFAPNFICAPELYNQYSIGSTLETSNQFCGKGTLDPM 420

Query: 421 KVKGKILVCLAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDR 480
           KVKGKI++C  GE  G +KG +A   GAAG+IV ND + GD+I+PELHF+PASD+  +D 
Sbjct: 421 KVKGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDA 480

Query: 481 QVLTRYMDSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNIL 540
           Q++ +Y+ ST+ P+ HL  V+T L +KPAP++A FSSRGPN I+ +ILKPD+TAPGVNIL
Sbjct: 481 QIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNIL 540

Query: 541 AAYSSGNAPTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTT 600
           A+Y +G APT S  DRRRIPF+VISGTSMSCPH++GIA L+KSIHP WSPAAIKSAIMTT
Sbjct: 541 ASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTT 600

Query: 601 AKTRDNNLGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAK 660
           AKTR NN  +ILD TK+KATP+AYGAG V+PNDA DPGLVYD T+ DYLNFLCARGY A 
Sbjct: 601 AKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAM 660

Query: 661 QIRRFYAKSYVCDRSFKLTDLNYPSISVSNLTMG-PVTVNRRVKNVGSLGTYVARVKVHP 720
           +I++FYAK + C RSFK+TDLNYPSISV  L +G P+T+NRRVKNVGS GTYVARVK  P
Sbjct: 661 KIKKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASP 720

Query: 721 EVSISVEPSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNL 772
            V++++EPSTL F SVGEEK FKVV Q  GK K  G +FGTLIWSDGKHFVRSPI ++L
Sbjct: 721 GVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVK-SGDVFGTLIWSDGKHFVRSPIAVHL 776

BLAST of MS028639 vs. ExPASy TrEMBL
Match: A0A6J1L3L8 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498821 PE=3 SV=1)

HSP 1 Score: 1023.1 bits (2644), Expect = 6.1e-295
Identity = 516/775 (66.58%), Postives = 620/775 (80.00%), Query Frame = 0

Query: 1   MEASNISP-LLLAFFLLSLLQTSTIA--IDKKSYIVYLGSQSSLASTSLLDSRQLTASHY 60
           MEASN+ P +L  FFL SLLQTSTIA    KK YIVYL SQS  A +     +Q+   HY
Sbjct: 1   MEASNLIPSILFLFFLFSLLQTSTIATIATKKPYIVYLESQSVYADSKHCSIQQVQEFHY 60

Query: 61  NLLGSLLGSKKIAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTT 120
           NLLGSLLGS + A+ESI YSYTRSFNGFAA L++  AA LA++PQVISV ENR RKLHTT
Sbjct: 61  NLLGSLLGSPEAAKESILYSYTRSFNGFAAILNEKEAADLARSPQVISVLENRGRKLHTT 120

Query: 121 HSWNFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKG 180
           +SW FLG+E+ +G+ P NSIWNAA+FG+D+I+ANIDTGVWPES SFSDEG+GPIPSKW+G
Sbjct: 121 NSWRFLGVEDDEGI-PSNSIWNAAQFGRDMIIANIDTGVWPESNSFSDEGYGPIPSKWRG 180

Query: 181 TCQTDLNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGA 240
           TC  D +F CNRKLIGGRYFYKGY+ AG  L+ +S+++RDH+GHGTHTL+TAAGNFVPGA
Sbjct: 181 TCPDDPSFRCNRKLIGGRYFYKGYKFAGGVLNATSVSIRDHNGHGTHTLSTAAGNFVPGA 240

Query: 241 NVFGQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGG 300
           N+FG GNGTAKGGAP+ARVAAYK CWP +PEGECFDAD LA FEAAI DGVDVIS SLGG
Sbjct: 241 NIFGHGNGTAKGGAPKARVAAYKACWPLIPEGECFDADVLAAFEAAINDGVDVISASLGG 300

Query: 301 SVRDFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASY 360
             ++F  DP+ IAAFHA QQGI+ +FSAGNDGP P +V NVAPW+ TVAAST  R+F S 
Sbjct: 301 GDQEFFRDPVGIAAFHAAQQGIIVIFSAGNDGPQPETVGNVAPWEITVAASTTGRNFVSN 360

Query: 361 IALGNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKG 420
           +ALGN K +KG+SL+SV+ LP +FYPLIDSV  +    NV++++A+FCG+GT +P KVKG
Sbjct: 361 VALGNNKILKGSSLSSVSALP-QFYPLIDSVNAKF--SNVSEFQAKFCGKGTLNPKKVKG 420

Query: 421 KILVCLAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLT 480
           KIL+C AG+I G EKG  A   GA G+I+  + D+ ++I PEL+FLPAS + YSD ++L 
Sbjct: 421 KILICTAGKITGVEKGYNAAEAGAVGMILATNIDNLEEIKPELYFLPASGITYSDGELLD 480

Query: 481 RYMDSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYS 540
            Y++ST  PV  +M VRTE+ I P+PV+A FSSRGPN  +K+ILKPDITAPG  ILA+Y 
Sbjct: 481 DYINSTSTPVAQIMNVRTEVEINPSPVVAAFSSRGPNPFDKAILKPDITAPGFTILASYP 540

Query: 541 SGNAPTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTR 600
           +  APT S +D+RR PF+V SGTSM+CPHIS IAALLKSIHP+WSPAAIKSA+MTTAKT 
Sbjct: 541 TTRAPTRSGFDQRRTPFNVFSGTSMACPHISAIAALLKSIHPEWSPAAIKSALMTTAKTS 600

Query: 601 DNNLGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRR 660
           DNN  ++L    + ATPFA+GAGHV PNDAMDPGLVYD TV +YLNFLCARGY A Q+RR
Sbjct: 601 DNN--NLL----LNATPFAFGAGHVRPNDAMDPGLVYDITVDEYLNFLCARGYNAIQLRR 660

Query: 661 FYAKSYVCDRSFKLTDLNYPSISVSNLTM-GPVTVNRRVKNVGSLGTYVARVKVHPEVSI 720
           F  + +VC++SFK  DLNYPSIS+ NL +  PVT+NRRVKNVG  GTYVARV++   V+ 
Sbjct: 661 FSKQPFVCEKSFKAIDLNYPSISIPNLNVNAPVTINRRVKNVGGPGTYVARVELPEGVAA 720

Query: 721 SVEPSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNL 772
           SVEPSTLQFSSVGEEKAF+VV Q  G+ K EG +FG L+WSDGKH V SPI MNL
Sbjct: 721 SVEPSTLQFSSVGEEKAFRVVVQNTGELKHEGYVFGKLVWSDGKHSVGSPISMNL 765

BLAST of MS028639 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 810.8 bits (2093), Expect = 9.2e-235
Identity = 415/771 (53.83%), Postives = 537/771 (69.65%), Query Frame = 0

Query: 5   NISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLLGSLL 64
           N S LLL   +    +    + D  SY+VY G+ S +   +     ++  +HY+ LGS  
Sbjct: 6   NFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFT 65

Query: 65  GSKKIAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTTHSWNFLG 124
           GS++ A ++IFYSYT+  NGFAA LD ++A  ++K+P+V+SVF N+A KLHTT SW+FLG
Sbjct: 66  GSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLG 125

Query: 125 MENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTCQT--D 184
           +E+   V P +SIW  A+FG+D I+AN+DTGVWPESKSF DEG GPIPS+WKG CQ   D
Sbjct: 126 LEHNSYV-PSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKD 185

Query: 185 LNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVFGQ 244
             FHCNRKLIG RYF KGY AA   L+ S  + RD DGHG+HTL+TAAG+FVPG ++FGQ
Sbjct: 186 ATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQ 245

Query: 245 GNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVRDF 304
           GNGTAKGG+PRARVAAYKVCWPP+   EC+DAD LA F+AAI DG DVIS SLGG    F
Sbjct: 246 GNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSF 305

Query: 305 ADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIALGN 364
            +D +AI +FHA ++ IV V SAGN GP   +VSNVAPW+ TV AST+DR+FAS + LGN
Sbjct: 306 FNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGN 365

Query: 365 KKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKILVC 424
            K  KG SL+S A    KFYP++ SV  +  ++N +   AQ C  G+ DP K KGKILVC
Sbjct: 366 GKHYKGQSLSSTALPHAKFYPIMASVNAK--AKNASALDAQLCKLGSLDPIKTKGKILVC 425

Query: 425 LAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTRYMDS 484
           L G+    EKG      G  G+++ N   +G+D+  + H LPA+ +   D   ++RY+  
Sbjct: 426 LRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQ 485

Query: 485 TRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGNAP 544
           T+ P+ H+   RT+LG+KPAPVMA FSS+GP+ +   ILKPDITAPGV+++AAY+   +P
Sbjct: 486 TKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSP 545

Query: 545 TTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNNLG 604
           T  ++D RR+ F+ ISGTSMSCPHISGIA LLK+ +P WSPAAI+SAIMTTA   D+  G
Sbjct: 546 TNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPG 605

Query: 605 SILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYAKS 664
            I + T +KATPF++GAGHV PN A++PGLVYD  +KDYLNFLC+ GY A QI  F   +
Sbjct: 606 PIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNN 665

Query: 665 YVCDR-SFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTYVARVKVHPEVSISVEPS 724
           + C      L +LNYPSI+V NLT   VTV+R VKNVG    Y  +V     V ++V+P+
Sbjct: 666 FTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPT 725

Query: 725 TLQFSSVGEEKAFKVVFQYKGKGK-REGSMFGTLIWSDGKHFVRSPIVMNL 772
           +L F+ VGE+K FKV+   K KG   +G +FG L+WSD KH VRSPIV+ L
Sbjct: 726 SLNFTKVGEQKTFKVIL-VKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of MS028639 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 797.0 bits (2057), Expect = 1.4e-230
Identity = 416/774 (53.75%), Postives = 526/774 (67.96%), Query Frame = 0

Query: 1   MEASNISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLL 60
           M   ++S LLL   L++L  +   A+ KKSYIVYLGS + L   S      +  SH   L
Sbjct: 16  MSLQSLSSLLL---LVTLFFSPAFAL-KKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFL 75

Query: 61  GSLLGSKKIAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTTHSW 120
            S +GS + A+E+IFYSY R  NGFAA LD+N AA +AK+P V+SVF N+ RKLHTTHSW
Sbjct: 76  ASFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSW 135

Query: 121 NFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTCQ 180
           NF+ +    GV   +S+WN A +G+D I+AN+DTGVWPESKSFSDEG+G +P++WKG C 
Sbjct: 136 NFMLLAK-NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH 195

Query: 181 TDLNFHCNRKLIGGRYFYKGYEA-AGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANV 240
            D+   CNRKLIG RYF KGY A  G   + S  T RDHDGHG+HTL+TAAGNFVPGANV
Sbjct: 196 KDV--PCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANV 255

Query: 241 FGQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSV 300
           FG GNGTA GG+P+ARVAAYKVCWPP+   ECFDAD LA  EAAI DGVDV+S S+GG  
Sbjct: 256 FGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDA 315

Query: 301 RDFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIA 360
            D+  D +AI +FHA++ G+  V SAGN GP  G+VSNVAPW  TV AS++DR+F +++ 
Sbjct: 316 GDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVE 375

Query: 361 LGNKKRIKGASLASVAPLP-KKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGK 420
           L N +  KG SL+   PLP +K Y LI +    + + NVTD  A  C +G+ DP KVKGK
Sbjct: 376 LKNGQSFKGTSLSK--PLPEEKMYSLISAADANVANGNVTD--ALLCKKGSLDPKKVKGK 435

Query: 421 ILVCLAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTR 480
           ILVCL G+    +KG++A   GAAG+++ NDK SG++I  + H LPAS + Y D + L  
Sbjct: 436 ILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFS 495

Query: 481 YMDSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSS 540
           Y+ ST++P G++      L  KPAP MA FSSRGPN I   ILKPDITAPGVNI+AA++ 
Sbjct: 496 YLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTE 555

Query: 541 GNAPTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRD 600
              PT    D RR PF+  SGTSMSCPHISG+  LLK++HP WSPAAI+SAIMTT++TR+
Sbjct: 556 ATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRN 615

Query: 601 NNLGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRF 660
           N    ++D +  KA PF+YG+GHV PN A  PGLVYD T  DYL+FLCA GY    ++ F
Sbjct: 616 NRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLF 675

Query: 661 YA-KSYVCDRSFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTYVARVKVHPEVSIS 720
                Y C +   L D NYPSI+V NLT G +TV R++KNVG   TY AR +    V +S
Sbjct: 676 AEDPQYTCRQGANLLDFNYPSITVPNLT-GSITVTRKLKNVGPPATYNARFREPLGVRVS 735

Query: 721 VEPSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNL 772
           VEP  L F+  GE K F++  +        G +FG L W+D  H+VRSPIV+ L
Sbjct: 736 VEPKQLTFNKTGEVKIFQMTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of MS028639 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 612.8 bits (1579), Expect = 3.7e-175
Identity = 347/771 (45.01%), Postives = 462/771 (59.92%), Query Frame = 0

Query: 12  AFFLLSLL---QTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLLGSLLGSKK 71
           AFFLL  L     S+ + D+ +YIV++      +S  L         H N   S L S  
Sbjct: 10  AFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDL---------HSNWYDSSLRSIS 69

Query: 72  IAEESIFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTTHSWNFLGMENA 131
            + E + Y+Y  + +GF+  L Q  A  L   P VISV      +LHTT +  FLG++  
Sbjct: 70  DSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH 129

Query: 132 QGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTCQTDLNFH-- 191
                   ++  A    D++V  +DTGVWPESKS+SDEGFGPIPS WKG C+   NF   
Sbjct: 130 TA-----DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTAS 189

Query: 192 -CNRKLIGGRYFYKGYEAAGAKL--SPSSLTVRDHDGHGTHTLATAAGNFVPGANVFGQG 251
            CNRKLIG R+F +GYE+    +  S  S + RD DGHGTHT +TAAG+ V GA++ G  
Sbjct: 190 LCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 249

Query: 252 NGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVRDFA 311
           +GTA+G APRARVA YKVCW     G CF +D LA  + AIAD V+V+S SLGG + D+ 
Sbjct: 250 SGTARGMAPRARVAVYKVCW----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYY 309

Query: 312 DDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIALGNK 371
            D +AI AF AM++GI+   SAGN GP+  S+SNVAPW  TV A T+DRDF +   LGN 
Sbjct: 310 RDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNG 369

Query: 372 KRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKILVCL 431
           K   G SL     LP K  P I   AG   + N T+     C  GT  P KVKGKI++C 
Sbjct: 370 KNFTGVSLFKGEALPDKLLPFI--YAGN--ASNATN--GNLCMTGTLIPEKVKGKIVMCD 429

Query: 432 AGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTRYMDST 491
            G     +KG      G  G+I+ N   +G+++  + H LPA+ V      ++  Y+ + 
Sbjct: 430 RGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTD 489

Query: 492 RNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGNAPT 551
            NP   +  + T +G+KP+PV+A FSSRGPN+I  +ILKPD+ APGVNILAA++    PT
Sbjct: 490 PNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPT 549

Query: 552 TSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNNLGS 611
               D RR+ F++ISGTSMSCPH+SG+AALLKS+HP+WSPAAI+SA+MTTA     +   
Sbjct: 550 GLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKP 609

Query: 612 ILDLTKVK-ATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYAKS 671
           +LD+   K +TPF +GAGHV P  A +PGL+YD T +DYL FLCA  YT+ QIR    ++
Sbjct: 610 LLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRN 669

Query: 672 YVCD--RSFKLTDLNYPSISVSNLTMGPVTVNRRVKNVGSLGTYVARVKVHPE-VSISVE 731
           Y CD  +S+ + DLNYPS +V+   +G     R V +VG  GTY  +V      V ISVE
Sbjct: 670 YTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVE 729

Query: 732 PSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMN 771
           P+ L F    E+K++ V F          + FG++ WSDGKH V SP+ ++
Sbjct: 730 PAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of MS028639 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 552.0 bits (1421), Expect = 7.7e-157
Identity = 316/764 (41.36%), Postives = 453/764 (59.29%), Query Frame = 0

Query: 31  YIVYLGSQSSLASTSLLDSRQLTA---SHYNLLGSLLGSKKIAEES-IFYSYTRSFNGFA 90
           +I +  SQ++      +D   + +   +HY+       S + AEES I + Y   F+GF+
Sbjct: 16  FISFAASQAAKTFIFRIDGGSMPSIFPTHYHWY-----STEFAEESRIVHVYHTVFHGFS 75

Query: 91  ATLDQNVAARLAKNPQVISVFENRARKLHTTHSWNFLGMENAQGVPPPNSIWNAAKFGQD 150
           A +  + A  L  +P V++VFE+R R+LHTT S  FLG++N +G      +W+ + +G D
Sbjct: 76  AVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKG------LWSESDYGSD 135

Query: 151 IIVANIDTGVWPESKSFSDEGFGPIPSKWKGTCQTDLNF---HCNRKLIGGRYFYKGYEA 210
           +I+   DTG+WPE +SFSD   GPIP +W+G C++   F   +CNRK+IG R+F KG +A
Sbjct: 136 VIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQA 195

Query: 211 A---GAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVFGQGNGTAKGGAPRARVAAYK 270
           A   G   +   L+ RD DGHGTHT +TAAG     A++ G  +G AKG AP+AR+AAYK
Sbjct: 196 AVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYK 255

Query: 271 VCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGS---VRDFADDPLAIAAFHAMQQ 330
           VCW    +  C D+D LA F+AA+ DGVDVIS S+GG       +  DP+AI ++ A  +
Sbjct: 256 VCW---KDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASK 315

Query: 331 GIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIALGNKKRIKGASLASVAPL 390
           GI    SAGN+GPN  SV+N+APW  TV ASTIDR+F +   LG+  R++G SL +  PL
Sbjct: 316 GIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPL 375

Query: 391 PKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKILVCLAGEIVGFEKGIEAM 450
             + +P++      + S       A  C E T DP +V+GKI++C  G      KG+   
Sbjct: 376 NGRMFPVVYPGKSGMSS-------ASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVK 435

Query: 451 RVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTRYMDSTRNPVGHLMKVRTEL 510
           + G  G+I+ N   +G+ +  + H +PA  V  ++   +  Y  S  NP+  +    T +
Sbjct: 436 KAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIV 495

Query: 511 GIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGNAPTTSRYDRRRIPFSVI 570
           GIKPAPV+A FS RGPN +   ILKPD+ APGVNILAA++    PT    D R+  F+++
Sbjct: 496 GIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNIL 555

Query: 571 SGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNNLGSILD-LTKVKATPFA 630
           SGTSM+CPH+SG AALLKS HP WSPA I+SA+MTT    DN+  S++D  T   ATP+ 
Sbjct: 556 SGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYD 615

Query: 631 YGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYAKSYVCDRSFKLT--DL 690
           YG+GH++   AM+PGLVYD T  DY+ FLC+ GY  K I+        C  + K +  +L
Sbjct: 616 YGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNL 675

Query: 691 NYPSIS----VSNLTMGPVTVNRRVKNVGSL-GTYVARVKVHPEVSISVEPSTLQFSSVG 750
           NYPSI+     +   +   TV R   NVG     Y AR++    V+++V+P  L F+S  
Sbjct: 676 NYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAV 735

Query: 751 EEKAFKVVFQYKGKG---KREGSMFGTLIWSD-GKHFVRSPIVM 770
           + +++ V      +       G++FG++ W D GKH VRSPIV+
Sbjct: 736 KRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758

BLAST of MS028639 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 552.0 bits (1421), Expect = 7.7e-157
Identity = 320/789 (40.56%), Postives = 460/789 (58.30%), Query Frame = 0

Query: 8   PLLLAFFLLSLL----QTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNLLGSL 67
           P L     ++L+    +T+T    KK+Y++++    S       +  Q  +S  N   S+
Sbjct: 10  PFLFIILSINLIFLQAETTTQISTKKTYVIHM--DKSAMPLPYTNHLQWYSSKIN---SV 69

Query: 68  LGSKKIAEES----IFYSYTRSFNGFAATLDQNVAARLAKNPQVISVFENRARKLHTTHS 127
              K   EE     I Y+Y  +F+G AA L Q  A RL +   V++V      +LHTT S
Sbjct: 70  TQHKSQEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRS 129

Query: 128 WNFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSKWKGTC 187
             FLG+E  +       +W       D++V  +DTG+WPES+SF+D G  P+P+ W+G C
Sbjct: 130 PTFLGLERQES----ERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGAC 189

Query: 188 QTDLNF---HCNRKLIGGRYFYKGYEAAGAKLSP--SSLTVRDHDGHGTHTLATAAGNFV 247
           +T   F   +CNRK++G R FY+GYEAA  K+       + RD DGHGTHT AT AG+ V
Sbjct: 190 ETGKRFLKRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPV 249

Query: 248 PGANVFGQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTS 307
            GAN+FG   GTA+G A +ARVAAYKVCW     G CF +D L+  + A+ADGV V+S S
Sbjct: 250 KGANLFGFAYGTARGMAQKARVAAYKVCW----VGGCFSSDILSAVDQAVADGVQVLSIS 309

Query: 308 LGGSVRDFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDF 367
           LGG V  ++ D L+IA F AM+ G+    SAGN GP+P S++NV+PW  TV AST+DRDF
Sbjct: 310 LGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDF 369

Query: 368 ASYIALGNKKRIKGASL-ASVAPLPK-KFYPLIDSVAGRIISRNVTD-YRAQFCGEGTQD 427
            + + +G  +  KG SL      LPK K YPL+       + RN +      FC +G  D
Sbjct: 370 PATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLV------YLGRNASSPDPTSFCLDGALD 429

Query: 428 PAKVKGKILVCLAGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYS 487
              V GKI++C  G     +KG    R G  G+++ N   +G+++  + H LPA  V   
Sbjct: 430 RRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEK 489

Query: 488 DRQVLTRYMDSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVN 547
           + +++ +Y  +++     L  + T +GIKP+PV+A FSSRGPN +   ILKPD+ APGVN
Sbjct: 490 EGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVN 549

Query: 548 ILAAYSSGNAPTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIM 607
           ILAA++   AP++   D RR+ F+++SGTSMSCPH+SG+AAL+KS HP WSPAAIKSA+M
Sbjct: 550 ILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALM 609

Query: 608 TTAKTRDNNLGSILDLT-KVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGY 667
           TTA   DN    + D +    ++P+ +GAGH+ P  A DPGLVYD   ++Y  FLC +  
Sbjct: 610 TTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDL 669

Query: 668 TAKQIRRFYAKSYVCDRSFKLT------DLNYPSISV---SNLTMGPVTVNRRVKNVG-S 727
           +  Q++ F   S   +R+ K T      +LNYP+IS     N  +  +T+ R V NVG  
Sbjct: 670 SPSQLKVFTKHS---NRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPH 729

Query: 728 LGTYVARVKVHPEVSISVEPSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGK 770
           + +Y   V      S++V+P TL F+S  ++ ++ V F+ + + KR    FG L+W    
Sbjct: 730 ISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR--PEFGGLVWKSTT 774