MS028604 (gene) Bitter gourd (TR) v1

Overview
NameMS028604
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPhenylalanine ammonia-lyase
Locationscaffold78: 248669 .. 250804 (-)
RNA-Seq ExpressionMS028604
SyntenyMS028604
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACCCGTGAATTTTTCTGCTCCTCAGCTCTCTCAGGAGCCGATTCCATTCATCAATCTAAATCTTTCCCCTCTGTTTCAGCCTCCGATCATTGGGTCAAGGCTGCCGACGCCCTCCGTTGCCCCCATTTTGAGGAGGTCCGACGAATGGTGTCGGAGTTTCAGGAGGCTAAGACTGTTGACATTGAAGGCACCACCCTCACTGTTGCTCAGGTCACCGCGATTGCTCGCCGGAGTGACGTCAAAGTTAACCTCAATGAAGCCGTGGCTCGTGATCGTGTGGCCAAAAGTGCTAATTGGGTTGCTGAAAACATCTCTCGGGGAACAGACACATATGGAGTTACTACCGGCTTTGGCGCCACCTCCCACCGGCGTACCACCAAAACGGCCGACCTCCAGACGGAGCTTATTCGATTCCTCAACGCTGGAGTCATCGGAAAGGACAATCTCCCGTCGAGCTACTCAAAGGCGGCCATGCTTGTTAGAACAAACACTTTGATGCAAGGCTACTCGGGCATTCGCTGGGAAATCCTTGAAGCTGTGGTGAAGCTCATGAATGAGAATTTGATCCCCAAATTGCCTTTGAGAGGTACAATCACAGCTTCCGGCGATCTGGTTCCACTGTCTTATATCGCTGGCGTTATCACCGGAAGGCATAATTCCAAGGTCACCACAATTGAAGGCGAAGAGGTAACCGCCATGCAAGCTCTGAAGAGAGCTGGAGTTGCAGGCCCATTTGAGCTTCAAGCAAAGGAAGGTTTAGCTCTCGTCAATGGAACCGCGGTTGGGTCCGCAGTCGCTGCCACAGTCTGCTTCGACGCCAACATTTTGGCTCTATTAGCCGTCGTTCTCTCTGCTATGTTCTGCGAAGCCATGCTCGGCAAGCCGGAATTTGCAGACCCACTAACCCATAAGCTCAAGCACCACCCTGGCCAGATTGAATCCGCCGCCGCCATGGAGTTCCTCTTGGATCAGAGCGACTTCATGAAAGAAGCAAAAACCCGACATGAAAAGGACCCACTGACAAAGCCAAAACAAGATCGCTATGCACTTCGAACTTCCCCCCAATGGCTTGGCCCACAAATTGAAGTCATTCGAACGGCAACCCATTCGATTGAAAGGGAAATCAACTCCGTAAACGATAATCCTTTAATTGATGTCCATCGGGACCTCGCTCTCCACGGAGGAAACTTCCAGGGGACCCCAATCGGTGTCTCCATGGACAATCTCCGGATCGCCATCGCTGCAATCGGAAAATTAATGTTCGCCCAATTCTCTGAACTCGTCTGCGATCATTACAATAATGGATTGCCGTCTAATTTGAGCGGCGGGCCAAATCCAAGCTTGGACTACGGCTTCAAAGGAGCGGAAATCGCCATGGCTGCTTACTGCTCTGAGCTTCAATACTTGGCAAACCCTGTCACGACCCACGTCCAGAGCGCAGAACAACACAATCAGGATGTAAACTCTCTAGGTTTAATCTCAGCCAGAAAATCCGCCGAAGCGATCGAGATTCTCAAGCTCATGTCCGCTACCTACATGGTGGCGCTCTGCCAGGCGATAGATTTGAGACAACACGAGGAGAATATGAGGGAAGTCGTGAAGAATGCTCTTCTCCAAGCCGTCAGAAAAACCCTATACGTCGCCGAAGATGGATCCCTTCTCGAATCGCGATTCTGCGAGAAAGATCTTCTGCAAGTAATCGAGCACGAGGCGGTGTTCTCCTACATCGACGATCCCACGAACACCTCCTACGCCCTAATCCCCAAATTGCTGGAAGTTCTCGTCGAAAGAGCGCTGAAAGGCGCCTCAGCGGACAAGGGCGAGCATGGAAATGAAACAGGCGTCACCATCTTCAGGCGAATCCCGGGGTTTTTGGAGGAATTGAAGGCGAAATTGGAAGAAGAGGTGGAAAGCGCGAGAGGGAGATTTGAGAACGGGGATTTCGCAGTGGAAAACAGAATCAAGAAATGCAGTACTTACCCGATCTATCATTTCGTGAGGACGGAAGTGGGGACTCAGCTGCTCAGTGGGGCGAAGAGGGTGAGCCCTGGAGAAGATATAGAGAAAGTTGTGGACGCCATTAATGGAGGTAAAATCGGAGAAGTTGTGACCAAGTGTTTGAAAAATTAG

mRNA sequence

ATGGCCACCCGTGAATTTTTCTGCTCCTCAGCTCTCTCAGGAGCCGATTCCATTCATCAATCTAAATCTTTCCCCTCTGTTTCAGCCTCCGATCATTGGGTCAAGGCTGCCGACGCCCTCCGTTGCCCCCATTTTGAGGAGGTCCGACGAATGGTGTCGGAGTTTCAGGAGGCTAAGACTGTTGACATTGAAGGCACCACCCTCACTGTTGCTCAGGTCACCGCGATTGCTCGCCGGAGTGACGTCAAAGTTAACCTCAATGAAGCCGTGGCTCGTGATCGTGTGGCCAAAAGTGCTAATTGGGTTGCTGAAAACATCTCTCGGGGAACAGACACATATGGAGTTACTACCGGCTTTGGCGCCACCTCCCACCGGCGTACCACCAAAACGGCCGACCTCCAGACGGAGCTTATTCGATTCCTCAACGCTGGAGTCATCGGAAAGGACAATCTCCCGTCGAGCTACTCAAAGGCGGCCATGCTTGTTAGAACAAACACTTTGATGCAAGGCTACTCGGGCATTCGCTGGGAAATCCTTGAAGCTGTGGTGAAGCTCATGAATGAGAATTTGATCCCCAAATTGCCTTTGAGAGGTACAATCACAGCTTCCGGCGATCTGGTTCCACTGTCTTATATCGCTGGCGTTATCACCGGAAGGCATAATTCCAAGGTCACCACAATTGAAGGCGAAGAGGTAACCGCCATGCAAGCTCTGAAGAGAGCTGGAGTTGCAGGCCCATTTGAGCTTCAAGCAAAGGAAGGTTTAGCTCTCGTCAATGGAACCGCGGTTGGGTCCGCAGTCGCTGCCACAGTCTGCTTCGACGCCAACATTTTGGCTCTATTAGCCGTCGTTCTCTCTGCTATGTTCTGCGAAGCCATGCTCGGCAAGCCGGAATTTGCAGACCCACTAACCCATAAGCTCAAGCACCACCCTGGCCAGATTGAATCCGCCGCCGCCATGGAGTTCCTCTTGGATCAGAGCGACTTCATGAAAGAAGCAAAAACCCGACATGAAAAGGACCCACTGACAAAGCCAAAACAAGATCGCTATGCACTTCGAACTTCCCCCCAATGGCTTGGCCCACAAATTGAAGTCATTCGAACGGCAACCCATTCGATTGAAAGGGAAATCAACTCCGTAAACGATAATCCTTTAATTGATGTCCATCGGGACCTCGCTCTCCACGGAGGAAACTTCCAGGGGACCCCAATCGGTGTCTCCATGGACAATCTCCGGATCGCCATCGCTGCAATCGGAAAATTAATGTTCGCCCAATTCTCTGAACTCGTCTGCGATCATTACAATAATGGATTGCCGTCTAATTTGAGCGGCGGGCCAAATCCAAGCTTGGACTACGGCTTCAAAGGAGCGGAAATCGCCATGGCTGCTTACTGCTCTGAGCTTCAATACTTGGCAAACCCTGTCACGACCCACGTCCAGAGCGCAGAACAACACAATCAGGATGTAAACTCTCTAGGTTTAATCTCAGCCAGAAAATCCGCCGAAGCGATCGAGATTCTCAAGCTCATGTCCGCTACCTACATGGTGGCGCTCTGCCAGGCGATAGATTTGAGACAACACGAGGAGAATATGAGGGAAGTCGTGAAGAATGCTCTTCTCCAAGCCGTCAGAAAAACCCTATACGTCGCCGAAGATGGATCCCTTCTCGAATCGCGATTCTGCGAGAAAGATCTTCTGCAAGTAATCGAGCACGAGGCGGTGTTCTCCTACATCGACGATCCCACGAACACCTCCTACGCCCTAATCCCCAAATTGCTGGAAGTTCTCGTCGAAAGAGCGCTGAAAGGCGCCTCAGCGGACAAGGGCGAGCATGGAAATGAAACAGGCGTCACCATCTTCAGGCGAATCCCGGGGTTTTTGGAGGAATTGAAGGCGAAATTGGAAGAAGAGGTGGAAAGCGCGAGAGGGAGATTTGAGAACGGGGATTTCGCAGTGGAAAACAGAATCAAGAAATGCAGTACTTACCCGATCTATCATTTCGTGAGGACGGAAGTGGGGACTCAGCTGCTCAGTGGGGCGAAGAGGGTGAGCCCTGGAGAAGATATAGAGAAAGTTGTGGACGCCATTAATGGAGGTAAAATCGGAGAAGTTGTGACCAAGTGTTTGAAAAATTAG

Coding sequence (CDS)

ATGGCCACCCGTGAATTTTTCTGCTCCTCAGCTCTCTCAGGAGCCGATTCCATTCATCAATCTAAATCTTTCCCCTCTGTTTCAGCCTCCGATCATTGGGTCAAGGCTGCCGACGCCCTCCGTTGCCCCCATTTTGAGGAGGTCCGACGAATGGTGTCGGAGTTTCAGGAGGCTAAGACTGTTGACATTGAAGGCACCACCCTCACTGTTGCTCAGGTCACCGCGATTGCTCGCCGGAGTGACGTCAAAGTTAACCTCAATGAAGCCGTGGCTCGTGATCGTGTGGCCAAAAGTGCTAATTGGGTTGCTGAAAACATCTCTCGGGGAACAGACACATATGGAGTTACTACCGGCTTTGGCGCCACCTCCCACCGGCGTACCACCAAAACGGCCGACCTCCAGACGGAGCTTATTCGATTCCTCAACGCTGGAGTCATCGGAAAGGACAATCTCCCGTCGAGCTACTCAAAGGCGGCCATGCTTGTTAGAACAAACACTTTGATGCAAGGCTACTCGGGCATTCGCTGGGAAATCCTTGAAGCTGTGGTGAAGCTCATGAATGAGAATTTGATCCCCAAATTGCCTTTGAGAGGTACAATCACAGCTTCCGGCGATCTGGTTCCACTGTCTTATATCGCTGGCGTTATCACCGGAAGGCATAATTCCAAGGTCACCACAATTGAAGGCGAAGAGGTAACCGCCATGCAAGCTCTGAAGAGAGCTGGAGTTGCAGGCCCATTTGAGCTTCAAGCAAAGGAAGGTTTAGCTCTCGTCAATGGAACCGCGGTTGGGTCCGCAGTCGCTGCCACAGTCTGCTTCGACGCCAACATTTTGGCTCTATTAGCCGTCGTTCTCTCTGCTATGTTCTGCGAAGCCATGCTCGGCAAGCCGGAATTTGCAGACCCACTAACCCATAAGCTCAAGCACCACCCTGGCCAGATTGAATCCGCCGCCGCCATGGAGTTCCTCTTGGATCAGAGCGACTTCATGAAAGAAGCAAAAACCCGACATGAAAAGGACCCACTGACAAAGCCAAAACAAGATCGCTATGCACTTCGAACTTCCCCCCAATGGCTTGGCCCACAAATTGAAGTCATTCGAACGGCAACCCATTCGATTGAAAGGGAAATCAACTCCGTAAACGATAATCCTTTAATTGATGTCCATCGGGACCTCGCTCTCCACGGAGGAAACTTCCAGGGGACCCCAATCGGTGTCTCCATGGACAATCTCCGGATCGCCATCGCTGCAATCGGAAAATTAATGTTCGCCCAATTCTCTGAACTCGTCTGCGATCATTACAATAATGGATTGCCGTCTAATTTGAGCGGCGGGCCAAATCCAAGCTTGGACTACGGCTTCAAAGGAGCGGAAATCGCCATGGCTGCTTACTGCTCTGAGCTTCAATACTTGGCAAACCCTGTCACGACCCACGTCCAGAGCGCAGAACAACACAATCAGGATGTAAACTCTCTAGGTTTAATCTCAGCCAGAAAATCCGCCGAAGCGATCGAGATTCTCAAGCTCATGTCCGCTACCTACATGGTGGCGCTCTGCCAGGCGATAGATTTGAGACAACACGAGGAGAATATGAGGGAAGTCGTGAAGAATGCTCTTCTCCAAGCCGTCAGAAAAACCCTATACGTCGCCGAAGATGGATCCCTTCTCGAATCGCGATTCTGCGAGAAAGATCTTCTGCAAGTAATCGAGCACGAGGCGGTGTTCTCCTACATCGACGATCCCACGAACACCTCCTACGCCCTAATCCCCAAATTGCTGGAAGTTCTCGTCGAAAGAGCGCTGAAAGGCGCCTCAGCGGACAAGGGCGAGCATGGAAATGAAACAGGCGTCACCATCTTCAGGCGAATCCCGGGGTTTTTGGAGGAATTGAAGGCGAAATTGGAAGAAGAGGTGGAAAGCGCGAGAGGGAGATTTGAGAACGGGGATTTCGCAGTGGAAAACAGAATCAAGAAATGCAGTACTTACCCGATCTATCATTTCGTGAGGACGGAAGTGGGGACTCAGCTGCTCAGTGGGGCGAAGAGGGTGAGCCCTGGAGAAGATATAGAGAAAGTTGTGGACGCCATTAATGGAGGTAAAATCGGAGAAGTTGTGACCAAGTGTTTGAAAAATTAG

Protein sequence

MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKTVDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALLQAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERALKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCSTYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN
Homology
BLAST of MS028604 vs. NCBI nr
Match: XP_022147741.1 (phenylalanine ammonia-lyase-like [Momordica charantia])

HSP 1 Score: 1380.9 bits (3573), Expect = 0.0e+00
Identity = 710/711 (99.86%), Postives = 710/711 (99.86%), Query Frame = 0

Query: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60
           MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT
Sbjct: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120
           VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG
Sbjct: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSIYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240
           AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR
Sbjct: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240

Query: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300
           AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA
Sbjct: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540

Query: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600
           QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA
Sbjct: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600

Query: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660
           LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS
Sbjct: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660

Query: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 712
           TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN
Sbjct: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 711

BLAST of MS028604 vs. NCBI nr
Match: XP_038879437.1 (phenylalanine ammonia-lyase-like [Benincasa hispida])

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 628/711 (88.33%), Postives = 668/711 (93.95%), Query Frame = 0

Query: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60
           MA  EF CSSALS  +S+ QSKSFPSV ASDHWVKAA AL   HFEEV RM+ EFQEA+T
Sbjct: 1   MANSEFLCSSALSETESVPQSKSFPSVLASDHWVKAAKALSHTHFEEVCRMLLEFQEAQT 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120
           VDIEGTTLTVAQVTAIARRSDVKV LNEAVA DRVA SA WVAE +S+G D YGVTTGFG
Sbjct: 61  VDIEGTTLTVAQVTAIARRSDVKVILNEAVAHDRVANSAKWVAEKVSKGIDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           ATSHRRTTK ADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRW+ILE
Sbjct: 121 ATSHRRTTKMADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWDILE 180

Query: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240
           AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEG  +TAM+ALKR
Sbjct: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGNVITAMEALKR 240

Query: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300
           AG+A PFELQAKEGLALVNGTAVGSAVAATVC+DANILALL+V+LSA+FCEAMLGKPEFA
Sbjct: 241 AGIAKPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLSVILSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAME LL+QSDFMKEAKTR+E DPL+KPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMESLLEQSDFMKEAKTRNENDPLSKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV+RDLALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ+EENMREVVKN+LL
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNSLL 540

Query: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600
           Q +RKTLY+AE GSLLESRFCEKDLLQVI+ EAVFSYIDDPTNTSY LIPKLLEVLVE+A
Sbjct: 541 QTIRKTLYIAEYGSLLESRFCEKDLLQVIDREAVFSYIDDPTNTSYTLIPKLLEVLVEKA 600

Query: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660
           LKG +  K E+GNETG+ IF++IP FLEELKAKLE++VE+AR RFENG+F VENRIKKCS
Sbjct: 601 LKGIATSKIENGNETGIAIFKKIPEFLEELKAKLEKDVENARERFENGEFEVENRIKKCS 660

Query: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 712
           TYPIY FVR+EVGTQLLSGAK+VSPGEDIEKV++AI+ GK+G VV +CLKN
Sbjct: 661 TYPIYRFVRSEVGTQLLSGAKKVSPGEDIEKVMEAIDEGKLGNVVIECLKN 711

BLAST of MS028604 vs. NCBI nr
Match: XP_023516501.1 (phenylalanine ammonia-lyase-like [Cucurbita pepo subsp. pepo] >AXF54191.1 PAL1 [Cucurbita pepo])

HSP 1 Score: 1223.8 bits (3165), Expect = 0.0e+00
Identity = 627/711 (88.19%), Postives = 663/711 (93.25%), Query Frame = 0

Query: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60
           MA  EFFC S LS  DS+ QSKSF  VS SD+W KAA+AL   H EEV RM+SEFQ+A+ 
Sbjct: 1   MANSEFFCYSPLSQPDSVPQSKSFSFVSPSDYWNKAANALSHTHLEEVCRMLSEFQDARA 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120
           VDIEG TLTVAQVTAIARRSDVKVNLNEAVA DRVAKSA WVAE IS GTD YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSDVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKDNLPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240
           AVVKLMNENL+PKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEG+E+TAM+ALKR
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALKR 240

Query: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300
           AG+AGPFELQAKEGLALVNGTAVGSAVAATVC+DANILALLAVVLSA+FCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAMEFLL+QSDFMKEAK RHE +PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600
           Q +R+TLYV + GSL ESRFCEKDLLQVI+ E VFSYIDDPTNTSYALIPKLLEVLV+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALIPKLLEVLVQRA 600

Query: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660
           LKG +  K E+GNETG+ IF++IPGFLEELK KLE +VE+ARGRFENG+F VENRIKKCS
Sbjct: 601 LKGIATAKKENGNETGIAIFKQIPGFLEELKEKLENDVENARGRFENGEFLVENRIKKCS 660

Query: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 712
           TYPIY FVRTEVGTQLLSGAK+VSPGEDIEKVVDAIN GK+G VV +CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGSVVLECLKN 711

BLAST of MS028604 vs. NCBI nr
Match: KAG6590021.1 (Phenylalanine ammonia-lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 627/711 (88.19%), Postives = 662/711 (93.11%), Query Frame = 0

Query: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60
           MA  EFFCSS LS  DS+ QSKSF  VS SD+W KAA+AL   H EEV RM+SEFQ+A+ 
Sbjct: 1   MANSEFFCSSPLSQPDSVPQSKSFSFVSPSDYWNKAANALSHTHLEEVCRMLSEFQDARV 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120
           VDIEG TLTVAQVTAIARRSDVKVNLNEAVA DRVAKSA WVAE IS GTD YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSDVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKDNLPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240
           AVVKLMNENL+PKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEG+E+TAM+AL R
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALNR 240

Query: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300
           AG+AGPFELQAKEGLALVNGTAVGSAVAATVC+DANILALLAVVLSA+FCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAME LL+QSDFMKEAK RHE +PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMELLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600
           Q +R+TLYV + GSL ESRFCEKDLLQVI+ E VFSYIDDPTNTSYALIPKLLEVLV+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALIPKLLEVLVQRA 600

Query: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660
           LKG +  K E+GNETG+TIF++IPGFLEELK KLE +VE+ARGRFEN DF VENRIKKCS
Sbjct: 601 LKGIATAKNENGNETGITIFKQIPGFLEELKEKLENDVENARGRFENDDFLVENRIKKCS 660

Query: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 712
           TYPIY FVRTEVGTQLLSGAK+VSPGEDIEKVVDAIN GK+G VV +CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGSVVLECLKN 711

BLAST of MS028604 vs. NCBI nr
Match: XP_022961017.1 (phenylalanine ammonia-lyase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1222.2 bits (3161), Expect = 0.0e+00
Identity = 625/711 (87.90%), Postives = 662/711 (93.11%), Query Frame = 0

Query: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60
           MA  EFFCSS LS  DS+ QSKSF  VS SD+W KAA+AL   H EEV RM+SEFQ+A+ 
Sbjct: 1   MANSEFFCSSPLSQPDSVPQSKSFSFVSPSDYWNKAANALSHTHLEEVCRMLSEFQDARV 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120
           VDIEG TLTVAQVTAIARRSDVKVNLNEAVA DRVAKSA WVAE IS GTD YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSDVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKDNLPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240
           AVVKLMNENL+PKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEG+E+TAM+AL R
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALNR 240

Query: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300
           AG+AGPFELQAKEGLALVNGTAVGSAVAATVC+DANILALLAVVLSA+FCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAME LL+QSDFMKEAK RHE +PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMELLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600
           Q +R+TLYV + GSL ESRFCEKDLLQVI+ E VFSYIDDPTNTSYALIPKLLEVLV+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALIPKLLEVLVQRA 600

Query: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660
           LKG +  K E+GNETG+ IF++IPGFLEELK KLE +VE+ARGRFENG+F VENRIKKCS
Sbjct: 601 LKGIATAKNENGNETGIAIFKQIPGFLEELKEKLENDVENARGRFENGEFLVENRIKKCS 660

Query: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 712
           TYPIY FVRTEVGTQLLSGAK+VSPGEDIEKVVDAIN GK+G +V +CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGSIVLECLKN 711

BLAST of MS028604 vs. ExPASy Swiss-Prot
Match: P52777 (Phenylalanine ammonia-lyase OS=Pinus taeda OX=3352 GN=PAL PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 8.8e-253
Identity = 449/687 (65.36%), Postives = 544/687 (79.18%), Query Frame = 0

Query: 28  SASD--HWVKAADALRCPHFEEVRRMVSEFQEAKTVDIEGTTLTVAQVTAIARRSDVKVN 87
           S SD  +WV+AA A+   HFEEV+ MV  +  AK + IEG +LT++ V A+ARRS VKV 
Sbjct: 27  SGSDPLNWVRAAKAMEGSHFEEVKAMVDSYFGAKEISIEGKSLTISDVAAVARRSQVKVK 86

Query: 88  LNEAVARDRVAKSANWVAENISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGV 147
           L+ A A+ RV +S+NWV   +++GTDTYGVTTGFGATSHRRT + A+LQ ELIRFLNAGV
Sbjct: 87  LDAAAAKSRVEESSNWVLTQMTKGTDTYGVTTGFGATSHRRTNQGAELQKELIRFLNAGV 146

Query: 148 IGK---DNLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNENLIPKLPLRGTITA 207
           +GK   + L    ++AAMLVRTNTL+QGYSG+RW+ILE V KL+N  L PKLPLRGTITA
Sbjct: 147 LGKCPENVLSEDTTRAAMLVRTNTLLQGYSGVRWDILETVEKLLNAWLTPKLPLRGTITA 206

Query: 208 SGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKRAGVAGPFELQAKEGLALVNGTA 267
           SGDLVPLSYIAG++TGR NS+V + +G E++  +ALK+ G+  PFELQ KEGLA+VNGT+
Sbjct: 207 SGDLVPLSYIAGLLTGRPNSRVRSRDGIEMSGAEALKKVGLEKPFELQPKEGLAIVNGTS 266

Query: 268 VGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEF 327
           VG+A+A+ VCFDAN+LALL+ V+SAMFCE M GKPEF DPLTHKLKHHPGQ+E+AA ME+
Sbjct: 267 VGAALASIVCFDANVLALLSEVISAMFCEVMNGKPEFTDPLTHKLKHHPGQMEAAAIMEY 326

Query: 328 LLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVND 387
           +LD S +MK A   HE +PL KPKQDRY LRTSPQWLGPQ+E+IR+ATH IEREINSVND
Sbjct: 327 VLDGSSYMKHAAKLHEMNPLQKPKQDRYGLRTSPQWLGPQVEIIRSATHMIEREINSVND 386

Query: 388 NPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNL 447
           NP+IDV RD ALHGGNFQGTPIGVSMDNLR++I+AIGKLMFAQFSELV D+YN GLPSNL
Sbjct: 387 NPVIDVARDKALHGGNFQGTPIGVSMDNLRLSISAIGKLMFAQFSELVNDYYNGGLPSNL 446

Query: 448 SGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAE 507
           SGGPNPSLDYG KGAEIAMA+Y SEL YLANPVT+HVQSAEQHNQDVNSLGL+SARKSAE
Sbjct: 447 SGGPNPSLDYGLKGAEIAMASYTSELLYLANPVTSHVQSAEQHNQDVNSLGLVSARKSAE 506

Query: 508 AIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALLQAVRKTLYVAEDGSLLESRFCE 567
           AI+ILKLM +TY+ ALCQA+DLR  EENM   VK  + Q  +KTL    +G LL  RFCE
Sbjct: 507 AIDILKLMLSTYLTALCQAVDLRHLEENMLATVKQIVSQVAKKTLSTGLNGELLPGRFCE 566

Query: 568 KDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERALKGASADKGEHGNETGVTIFRR 627
           KDLLQV+++E VFSYIDDP N SY L  KL  +LVE A K A  +K     +   +IF +
Sbjct: 567 KDLLQVVDNEHVFSYIDDPCNASYPLTQKLRNILVEHAFKNAEGEK-----DPNTSIFNK 626

Query: 628 IPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCSTYPIYHFVRTEVGTQLLSGAKR 687
           IP F  ELKA+LE +V  AR  ++ G   + +RI++C +YP+Y FVR ++GT+LLSG + 
Sbjct: 627 IPVFEAELKAQLEPQVSLARESYDKGTSPLPDRIQECRSYPLYEFVRNQLGTKLLSGTRT 686

Query: 688 VSPGEDIEKVVDAINGGKIGEVVTKCL 710
           +SPGE IE V DAI+  K+   + KCL
Sbjct: 687 ISPGEVIEVVYDAISEDKVIVPLFKCL 708

BLAST of MS028604 vs. ExPASy Swiss-Prot
Match: A0A2H5AIW0 (Phenylalanine ammonia-lyase 1 OS=Narcissus pseudonarcissus OX=39639 GN=PAL1 PE=2 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 6.5e-240
Identity = 436/686 (63.56%), Postives = 535/686 (77.99%), Query Frame = 0

Query: 33  WVKAADALRCPHFEEVRRMVSEFQEAKTVDIEGTTLTVAQVTAIAR-RSDVKVNLNEAVA 92
           W  AA+AL   H EEV+RMV E +EA  + + G TL V Q+ A+A   S  KV L+E+ A
Sbjct: 21  WGAAAEALAGSHLEEVKRMVKEHREA-CIRLGGATLKVGQIAAVANGGSAAKVELDES-A 80

Query: 93  RDRVAKSANWVAENISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGK--- 152
           R RV  S++WV +++S+GTD+YGVTTGFGATSHRRT +   LQ ELIRFLNAG+ G    
Sbjct: 81  RARVKASSDWVMDSMSKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGSGRD 140

Query: 153 --DNLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNENLIPKLPLRGTITASGDL 212
             + LP+S ++AAMLVR NTL+QGYSGIR+EILEA+ +L+N N+ P LPLRGTITASGDL
Sbjct: 141 SCNTLPASTTRAAMLVRINTLLQGYSGIRFEILEAITRLLNNNVTPCLPLRGTITASGDL 200

Query: 213 VPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKRAGVA-GPFELQAKEGLALVNGTAVGS 272
           VPLSYIAG++TGR NSK T  +G ++ A +A + AG+  G F+LQ KEGLALVNGTAVGS
Sbjct: 201 VPLSYIAGILTGRPNSKATAPDGAQIDASEAFRLAGITDGFFQLQPKEGLALVNGTAVGS 260

Query: 273 AVAATVCFDANILALLAVVLSAMFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFLLD 332
            +A+TV +DANILA+L+ V SA+FCE M GKPEF D LTHKLKHHPGQIE+AA ME +LD
Sbjct: 261 GLASTVLYDANILAVLSEVFSAIFCEVMQGKPEFTDHLTHKLKHHPGQIEAAAVMEHILD 320

Query: 333 QSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDNPL 392
            S +MK AK  HE DPL KPKQDRYALRTSPQWLGPQIEVIR+AT SIEREINSVNDNPL
Sbjct: 321 GSSYMKMAKKLHELDPLQKPKQDRYALRTSPQWLGPQIEVIRSATKSIEREINSVNDNPL 380

Query: 393 IDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLSGG 452
           IDV R+ ALHGGNFQGTPIGVSMDN R+AIA+IGKLMFAQFSELV D YNNGLPSNLSGG
Sbjct: 381 IDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNLSGG 440

Query: 453 PNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIE 512
            +PSLDYGFKGAEIAMA+YCSELQ+LANPVT HVQSAEQHNQDVNSLGLISARK+ EA+ 
Sbjct: 441 RDPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISARKTEEAVH 500

Query: 513 ILKLMSATYMVALCQAIDLRQHEENMREVVKNALLQAVRKTLYVAEDGSLLESRFCEKDL 572
           ILKLMS T++V LCQAIDLR  EEN++  VK  + Q  ++ L    +G L  SRFCEKDL
Sbjct: 501 ILKLMSTTFLVGLCQAIDLRHLEENLKSTVKTTISQVAKRVLTSGINGELHPSRFCEKDL 560

Query: 573 LQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERALKGASADKGEHGNETGVTIFRRIPG 632
           ++V++ E VFSY+DDP + +Y L+ +L ++LVE A+     + GE   +   +IFR+I  
Sbjct: 561 IKVVDREHVFSYVDDPCSATYPLMQRLRQILVEHAM-----NNGEKEKDADTSIFRKISA 620

Query: 633 FLEELKAKLEEEVESARGRFENGDFAVENRIKKCSTYPIYHFVRTEVGTQLLSGAKRVSP 692
           F +ELK  L +EVESAR  +ENG  A++NRI++C +YP+Y FVR E GT LL+G    SP
Sbjct: 621 FEDELKVVLPKEVESARVAYENGTSAIKNRIEECRSYPLYRFVREETGTSLLTGENVRSP 680

Query: 693 GEDIEKVVDAINGGKIGEVVTKCLKN 712
           GE+ +KV +AI  G++ + + +CL++
Sbjct: 681 GEEFDKVFNAICEGRLVDPLLECLED 699

BLAST of MS028604 vs. ExPASy Swiss-Prot
Match: Q50EX7 (Phenylalanine ammonia-lyase 1 OS=Petunia hybrida OX=4102 GN=PAL1 PE=1 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 8.5e-240
Identity = 442/687 (64.34%), Postives = 534/687 (77.73%), Query Frame = 0

Query: 32  HWVKAADALRCPHFEEVRRMVSEFQEAKTVDIEGTTLTVAQVTAIARRS--DVKVNLNEA 91
           +W  AADAL+  H +EV+RMV EF++   V + G TLTVAQV AIA +S  DV V L+EA
Sbjct: 29  NWNMAADALKGSHLDEVKRMVKEFRK-PVVRLGGETLTVAQVAAIASQSGTDVTVQLSEA 88

Query: 92  VARDRVAKSANWVAENISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIG-- 151
            +R  V  S++WV + +  GTD+YGVTTGFGATSHRRT + A LQ ELIRFLNAG+ G  
Sbjct: 89  -SRAGVKASSDWVLQGMINGTDSYGVTTGFGATSHRRTKEGAALQKELIRFLNAGIFGNG 148

Query: 152 ---KDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNENLIPKLPLRGTITASG 211
                 LP S ++AAMLVR NTL+QGYSGIR+EILEA+ KL+N N+ P LPLRGTITASG
Sbjct: 149 TESSHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNITPCLPLRGTITASG 208

Query: 212 DLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKRAGV-AGPFELQAKEGLALVNGTAV 271
           DLVPLSYIAG++TGR NSK T   GE + A++A +RAG+  G FELQ KEGLALVNGTAV
Sbjct: 209 DLVPLSYIAGLLTGRPNSKATGPNGELLDAVKAFQRAGIDTGFFELQPKEGLALVNGTAV 268

Query: 272 GSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFL 331
           GS +A+ V FDANILA+L+ VLSA+F E M GKPEF D LTHKLKHHPGQIE+AA ME +
Sbjct: 269 GSGLASMVLFDANILAVLSEVLSAIFAEVMQGKPEFTDYLTHKLKHHPGQIEAAAIMEHI 328

Query: 332 LDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDN 391
           LD S ++KEAK  HE DPL KPKQDRYALRTSPQWLGPQIEVIR AT  IEREINSVNDN
Sbjct: 329 LDGSSYVKEAKIVHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIEREINSVNDN 388

Query: 392 PLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLS 451
           PLIDV R+ ALHGGNFQGTPIGVSMDN R+A+A+IGKLMFAQFSELV D+YNNGLPSNL+
Sbjct: 389 PLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKLMFAQFSELVNDYYNNGLPSNLT 448

Query: 452 GGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEA 511
           GG NPSLDYGFKGAEIAMA+YCSELQ+LANPVT HVQSAEQHNQDVNSLGLIS+RK+AEA
Sbjct: 449 GGRNPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEA 508

Query: 512 IEILKLMSATYMVALCQAIDLRQHEENMREVVKNALLQAVRKTLYVAEDGSLLESRFCEK 571
           ++ILKLMS+TY+VALCQAIDLR  EEN++  VKN++    +K L + E G L  SRFCEK
Sbjct: 509 VDILKLMSSTYLVALCQAIDLRHLEENLKATVKNSVSLVAKKVLTIGEKGELHHSRFCEK 568

Query: 572 DLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERALKGASADKGEHGNETGVTIFRRI 631
           D+L+V++ E +F+Y DD  + +Y L+ KL +VLV+RAL     +K     ++  +IF++I
Sbjct: 569 DMLKVVDREYIFAYADDACSATYPLMQKLRQVLVDRALLNVDGEK-----DSSTSIFQKI 628

Query: 632 PGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCSTYPIYHFVRTEVGTQLLSGAKRV 691
             F EELK  L +E+E AR   E G  A+ NRI++C +YP+Y FVR E+    L+G K  
Sbjct: 629 KAFEEELKVVLPKEIERARSDLEQGKPAIPNRIQECRSYPLYKFVREELKANYLTGEKVQ 688

Query: 692 SPGEDIEKVVDAINGGKIGEVVTKCLK 711
           SPGE+ +KV  A+N GK+ + +  CLK
Sbjct: 689 SPGEEFDKVFTAMNEGKLVDPLLNCLK 708

BLAST of MS028604 vs. ExPASy Swiss-Prot
Match: P45726 (Phenylalanine ammonia-lyase OS=Camellia sinensis OX=4442 GN=PAL PE=2 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 4.2e-239
Identity = 439/686 (63.99%), Postives = 536/686 (78.13%), Query Frame = 0

Query: 32  HWVKAADALRCPHFEEVRRMVSEFQEAKTVDIEGTTLTVAQVTAIA-RRSDVKVNLNEAV 91
           +W  AA+A++  H EEV+ MV EF++   V + G TLT++QV AIA R S+V V L+E+ 
Sbjct: 26  NWGVAAEAMKGSHLEEVKGMVEEFRK-PVVRLGGETLTISQVAAIAVRGSEVAVELSES- 85

Query: 92  ARDRVAKSANWVAENISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGK-- 151
           AR+ V  S++WV E++++GTD+YGVTTGFGATSHRRT +   LQ ELIRFLNAG+ G   
Sbjct: 86  AREGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKEGGALQKELIRFLNAGIFGNGT 145

Query: 152 ---DNLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNENLIPKLPLRGTITASGD 211
                LP S ++AAMLVR NTL+QGYSGIR+EILEA+ K +N N+ P LPLRGTITASGD
Sbjct: 146 ESCHTLPQSATRAAMLVRINTLLQGYSGIRFEILEAISKFLNNNITPCLPLRGTITASGD 205

Query: 212 LVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKRAGVAGP-FELQAKEGLALVNGTAVG 271
           LVPLSYIAG++TGRHNSK     GE +   +A + AGV G  FELQ KEGLALVNGTAVG
Sbjct: 206 LVPLSYIAGLLTGRHNSKAVGPTGEILHPKEAFRLAGVEGGFFELQPKEGLALVNGTAVG 265

Query: 272 SAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFLL 331
           S +A+ V F+ANILA+L+ VLSA+F E M GKPEF D LTHKLKHHPGQIE+AA ME +L
Sbjct: 266 SGLASMVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHIL 325

Query: 332 DQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDNP 391
           D S ++K A+  HE DPL KPKQDRYALRTSPQWLGP IEVIR++T SIEREINSVNDNP
Sbjct: 326 DGSSYVKAAQKLHEMDPLQKPKQDRYALRTSPQWLGPLIEVIRSSTKSIEREINSVNDNP 385

Query: 392 LIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLSG 451
           LI+V R+ ALHGGNFQGTPIGVSMDN R+A+A+IGKLMFAQFSELV D YNNGLPSNLSG
Sbjct: 386 LINVSRNKALHGGNFQGTPIGVSMDNTRLAVASIGKLMFAQFSELVNDFYNNGLPSNLSG 445

Query: 452 GPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAI 511
           G NPSLDYGFKGAEIAMAAYCSELQ+LANPVT HVQSAEQHNQDVNSLGLIS+RK+AEA+
Sbjct: 446 GRNPSLDYGFKGAEIAMAAYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAV 505

Query: 512 EILKLMSATYMVALCQAIDLRQHEENMREVVKNALLQAVRKTLYVAEDGSLLESRFCEKD 571
           +ILKLMS+TY+VALCQA+DLR  EEN+R  VK+ + Q  ++ L +  +G L  SRFCEKD
Sbjct: 506 DILKLMSSTYLVALCQAVDLRHFEENLRNTVKSTVSQVAKRVLTMGVNGELHPSRFCEKD 565

Query: 572 LLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERALKGASADKGEHGNETGVTIFRRIP 631
           LL+V++ E +F+YIDDP + +Y L+ KL +VLVE ALK      GE       +IF++I 
Sbjct: 566 LLRVVDREYIFAYIDDPCSATYPLMQKLRQVLVEHALK-----NGESEKNLSTSIFQKIR 625

Query: 632 GFLEELKAKLEEEVESARGRFENGDFAVENRIKKCSTYPIYHFVRTEVGTQLLSGAKRVS 691
            F EE+K  L +EVES R   ENG+ A+ NRIK+C +YP+Y FVR E+GT+LL+G K  S
Sbjct: 626 AFEEEIKTLLPKEVESTRAAIENGNSAIPNRIKECRSYPLYKFVREELGTELLTGEKVRS 685

Query: 692 PGEDIEKVVDAINGGKIGEVVTKCLK 711
           PGE+ +KV  A+  G++ + +  CLK
Sbjct: 686 PGEEFDKVFTALCKGEMIDPLMDCLK 704

BLAST of MS028604 vs. ExPASy Swiss-Prot
Match: O23865 (Phenylalanine ammonia-lyase 1 OS=Daucus carota OX=4039 GN=PAL1 PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 5.5e-239
Identity = 441/686 (64.29%), Postives = 535/686 (77.99%), Query Frame = 0

Query: 32  HWVKAADALRCPHFEEVRRMVSEFQEAKTVDIEGTTLTVAQVTAIARRSDVKVNLNEAVA 91
           +W  AA+AL   H +EV+RMV+EF++   V + G TLTV+QV AIA  S VKV L E+ A
Sbjct: 22  NWGMAAEALTGSHLDEVKRMVAEFRK-PMVQLGGETLTVSQVAAIAAGS-VKVELAES-A 81

Query: 92  RDRVAKSANWVAENISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGKDN- 151
           R  V  S++WV E++++GTD+YGVTTGFGATSHRRT +   LQ ELIRFLNAG+ G  N 
Sbjct: 82  RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGSGND 141

Query: 152 ----LPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNENLIPKLPLRGTITASGDL 211
               LP S ++AAMLVR NTL+QGYSGIR+EILEA+ K +N+N+ P LPLRGTITASGDL
Sbjct: 142 SSNILPHSATRAAMLVRINTLLQGYSGIRFEILEAITKFLNQNITPCLPLRGTITASGDL 201

Query: 212 VPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKRAGV-AGPFELQAKEGLALVNGTAVGS 271
           VPLSYIAG++TGR NSK     GE +TA +A K AGV  G FELQ KEGLALVNGTAVGS
Sbjct: 202 VPLSYIAGLLTGRPNSKAVGPTGENLTAAEAFKLAGVDGGFFELQPKEGLALVNGTAVGS 261

Query: 272 AVAATVCFDANILALLAVVLSAMFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFLLD 331
            +A+ V F+ NILA+LA V+SA+F E M GKPEF D LTHKLKHHPGQIE+AA ME +LD
Sbjct: 262 GMASMVLFETNILAVLAEVMSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILD 321

Query: 332 QSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDNPL 391
            S ++K A+ +HE DPL KPKQDRYALRTSPQWLGPQIEVIR++T  IEREINSVNDNPL
Sbjct: 322 GSSYVKAAEKQHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRSSTKMIEREINSVNDNPL 381

Query: 392 IDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLSGG 451
           IDV R+ A+HGGNFQGTPIGVSMDN R+AIAAIGKLMFAQFSELV D YNNGLPSNLSGG
Sbjct: 382 IDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLSGG 441

Query: 452 PNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIE 511
            NPSLDYGFKGAEIAMA+YCSELQ+L NPVT HVQSAEQHNQDVNSLGLIS+RK+AEA+E
Sbjct: 442 RNPSLDYGFKGAEIAMASYCSELQFLGNPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVE 501

Query: 512 ILKLMSATYMVALCQAIDLRQHEENMREVVKNALLQAVRKTLYVAEDGSLLESRFCEKDL 571
           ILKLMS T++V LCQA+DLR  EEN++  VKN + Q  +K L +  +G L  SRFCE DL
Sbjct: 502 ILKLMSTTFLVGLCQAVDLRHLEENLKSTVKNTVSQVAKKVLTMGVNGELHPSRFCELDL 561

Query: 572 LQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERALKGASADKGEHGNETGVTIFRRIPG 631
           L+V++ E +F+YIDDP + +Y L+ KL +VLVE ALK      GE       +IF++I  
Sbjct: 562 LRVVDREYIFAYIDDPCSATYPLMQKLRQVLVEHALK-----NGETEKNLSTSIFQKIAA 621

Query: 632 FLEELKAKLEEEVESARGRFENGDFAVENRIKKCSTYPIYHFVRTEVGTQLLSGAKRVSP 691
           F +ELKA L +EVESAR   E+G+ A+ NRIK+C +YP+Y F+R E+GT  L+G K  SP
Sbjct: 622 FEDELKALLPKEVESARAVVESGNPAIPNRIKECRSYPLYKFIREELGTVYLTGEKVTSP 681

Query: 692 GEDIEKVVDAINGGKIGEVVTKCLKN 712
           GE+ +KV  A++ G+I + +  CL++
Sbjct: 682 GEEFDKVFTAMSKGEIIDPLLACLES 699

BLAST of MS028604 vs. ExPASy TrEMBL
Match: A0A6J1D395 (Phenylalanine ammonia-lyase OS=Momordica charantia OX=3673 GN=LOC111016602 PE=3 SV=1)

HSP 1 Score: 1380.9 bits (3573), Expect = 0.0e+00
Identity = 710/711 (99.86%), Postives = 710/711 (99.86%), Query Frame = 0

Query: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60
           MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT
Sbjct: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120
           VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG
Sbjct: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSIYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240
           AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR
Sbjct: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240

Query: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300
           AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA
Sbjct: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540

Query: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600
           QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA
Sbjct: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600

Query: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660
           LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS
Sbjct: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660

Query: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 712
           TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN
Sbjct: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 711

BLAST of MS028604 vs. ExPASy TrEMBL
Match: A0A345BTD4 (Phenylalanine ammonia-lyase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 1223.8 bits (3165), Expect = 0.0e+00
Identity = 627/711 (88.19%), Postives = 663/711 (93.25%), Query Frame = 0

Query: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60
           MA  EFFC S LS  DS+ QSKSF  VS SD+W KAA+AL   H EEV RM+SEFQ+A+ 
Sbjct: 1   MANSEFFCYSPLSQPDSVPQSKSFSFVSPSDYWNKAANALSHTHLEEVCRMLSEFQDARA 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120
           VDIEG TLTVAQVTAIARRSDVKVNLNEAVA DRVAKSA WVAE IS GTD YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSDVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKDNLPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240
           AVVKLMNENL+PKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEG+E+TAM+ALKR
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALKR 240

Query: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300
           AG+AGPFELQAKEGLALVNGTAVGSAVAATVC+DANILALLAVVLSA+FCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAMEFLL+QSDFMKEAK RHE +PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600
           Q +R+TLYV + GSL ESRFCEKDLLQVI+ E VFSYIDDPTNTSYALIPKLLEVLV+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALIPKLLEVLVQRA 600

Query: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660
           LKG +  K E+GNETG+ IF++IPGFLEELK KLE +VE+ARGRFENG+F VENRIKKCS
Sbjct: 601 LKGIATAKKENGNETGIAIFKQIPGFLEELKEKLENDVENARGRFENGEFLVENRIKKCS 660

Query: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 712
           TYPIY FVRTEVGTQLLSGAK+VSPGEDIEKVVDAIN GK+G VV +CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGSVVLECLKN 711

BLAST of MS028604 vs. ExPASy TrEMBL
Match: A0A6J1H8Z9 (Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111461649 PE=3 SV=1)

HSP 1 Score: 1222.2 bits (3161), Expect = 0.0e+00
Identity = 625/711 (87.90%), Postives = 662/711 (93.11%), Query Frame = 0

Query: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60
           MA  EFFCSS LS  DS+ QSKSF  VS SD+W KAA+AL   H EEV RM+SEFQ+A+ 
Sbjct: 1   MANSEFFCSSPLSQPDSVPQSKSFSFVSPSDYWNKAANALSHTHLEEVCRMLSEFQDARV 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120
           VDIEG TLTVAQVTAIARRSDVKVNLNEAVA DRVAKSA WVAE IS GTD YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSDVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKDNLPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240
           AVVKLMNENL+PKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEG+E+TAM+AL R
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALNR 240

Query: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300
           AG+AGPFELQAKEGLALVNGTAVGSAVAATVC+DANILALLAVVLSA+FCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAME LL+QSDFMKEAK RHE +PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMELLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600
           Q +R+TLYV + GSL ESRFCEKDLLQVI+ E VFSYIDDPTNTSYALIPKLLEVLV+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALIPKLLEVLVQRA 600

Query: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660
           LKG +  K E+GNETG+ IF++IPGFLEELK KLE +VE+ARGRFENG+F VENRIKKCS
Sbjct: 601 LKGIATAKNENGNETGIAIFKQIPGFLEELKEKLENDVENARGRFENGEFLVENRIKKCS 660

Query: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 712
           TYPIY FVRTEVGTQLLSGAK+VSPGEDIEKVVDAIN GK+G +V +CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGSIVLECLKN 711

BLAST of MS028604 vs. ExPASy TrEMBL
Match: A0A6J1JJE5 (Phenylalanine ammonia-lyase OS=Cucurbita maxima OX=3661 GN=LOC111485128 PE=3 SV=1)

HSP 1 Score: 1220.7 bits (3157), Expect = 0.0e+00
Identity = 625/711 (87.90%), Postives = 662/711 (93.11%), Query Frame = 0

Query: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60
           MA  EFFCSS L   DS+ QSKSF SVS SD+W KAA+AL   H EEV RM+SEFQ+ + 
Sbjct: 1   MANSEFFCSSPLYQPDSVPQSKSFSSVSPSDYWNKAANALSHTHLEEVCRMLSEFQDTRA 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120
           VDIEG TLTVAQVTAIARRS+VKVNLNEAVA DRVAKSA WVAE IS GTD YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSNVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKDNLPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240
           AVVKLMNENL+PKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEG+E+TAM+AL R
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALNR 240

Query: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300
           AG+AGPFELQAKEGLALVNGTAVGSAVAATVC+DANILALLAVVLSA+FCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAMEFLL+QSDFMKEAK RHE +PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600
           Q +R+TLYV + GSL ESRFCEKDLLQVI+ E VFSYIDDPTNTSYAL+PKLLEVLV+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALMPKLLEVLVQRA 600

Query: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660
           LKG +  K E+GNETGV IF++IPGFLEELK KLE +VE+ARGRFENG+F VENRIKKCS
Sbjct: 601 LKGIATAKNENGNETGVAIFKQIPGFLEELKEKLENDVETARGRFENGEFLVENRIKKCS 660

Query: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 712
           TYPIY FVRTEVGTQLLSGAK+VSPGEDIEKVVDAIN GK+G VV +CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGNVVLECLKN 711

BLAST of MS028604 vs. ExPASy TrEMBL
Match: A0A0A0LYZ4 (Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_1G590300 PE=3 SV=1)

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 611/711 (85.94%), Postives = 653/711 (91.84%), Query Frame = 0

Query: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60
           MA  +F CSSA S    +  SKS  S S SDHWVKAA+AL   HFEEV RM+SEFQEA+T
Sbjct: 1   MANSQFLCSSARSKTGFVPLSKSVSSDSTSDHWVKAANALSQTHFEEVCRMLSEFQEAQT 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120
           VD+EGTTLTVAQV  IARRSDVKV LNE+VA DRVA+SA WVAE +S G D YGVTTGFG
Sbjct: 61  VDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVAESAKWVAEKVSSGIDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           ATSH RTTK +DLQTELIRFLNAGVIGKDNLPS YSK AMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 ATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYSKVAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240
           A+VKLMNENLIPKLPLRGTITASGDLVPLSYIAGV+TGRHNSKVTTIEG+E+TA++ALKR
Sbjct: 181 ALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTIEGDEITAIEALKR 240

Query: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300
           AGVAGPFELQAKEGLALVNGTAVGSAVAATVC+DANILALLA VLSA+FCEAMLGKPEF 
Sbjct: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLASVLSALFCEAMLGKPEFT 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAA MEFLL+QSDFMKEAK  +E DPLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKICNENDPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV RD+A+HGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVC HYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVVKNALL
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQFEENMREVVKNALL 540

Query: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600
           Q +RKTLY+AE GSLLESRFCEKDLLQVIE E VFSYIDDPTNTSY LIPKLLEVLVE+A
Sbjct: 541 QTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFSYIDDPTNTSYTLIPKLLEVLVEKA 600

Query: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660
           LKG +  K ++GNETG+ IF++IP FLEELKAKLE++VE+ARGRFENGDF +ENRIKKCS
Sbjct: 601 LKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEKDVENARGRFENGDFEIENRIKKCS 660

Query: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 712
           TYPIY FVR EVGTQLLSGAK+VSPGEDIEKVV+AI+ GK+G ++ +CLKN
Sbjct: 661 TYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAIDEGKLGNILMECLKN 711

BLAST of MS028604 vs. TAIR 10
Match: AT3G53260.1 (phenylalanine ammonia-lyase 2 )

HSP 1 Score: 821.6 bits (2121), Expect = 4.8e-238
Identity = 431/686 (62.83%), Postives = 531/686 (77.41%), Query Frame = 0

Query: 32  HWVKAADALRCPHFEEVRRMVSEFQEAKTVDIEGTTLTVAQVTAIAR-RSDVKVNLNEAV 91
           +W  AAD ++  H +EV++MV E++    V++ G TLT+ QV AI+     VKV L E  
Sbjct: 29  NWGLAADQMKGSHLDEVKKMVEEYRR-PVVNLGGETLTIGQVAAISTVGGSVKVELAE-T 88

Query: 92  ARDRVAKSANWVAENISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGK-- 151
           +R  V  S++WV E++++GTD+YGVTTGFGATSHRRT     LQTELIRFLNAG+ G   
Sbjct: 89  SRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGTALQTELIRFLNAGIFGNTK 148

Query: 152 ---DNLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNENLIPKLPLRGTITASGD 211
                LP S ++AAMLVR NTL+QGYSGIR+EILEA+  L+N N+ P LPLRGTITASGD
Sbjct: 149 ETCHTLPQSATRAAMLVRVNTLLQGYSGIRFEILEAITSLLNHNISPSLPLRGTITASGD 208

Query: 212 LVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKRAGVA-GPFELQAKEGLALVNGTAVG 271
           LVPLSYIAG++TGR NSK T  +GE +TA +A ++AG++ G F+LQ KEGLALVNGTAVG
Sbjct: 209 LVPLSYIAGLLTGRPNSKATGPDGESLTAKEAFEKAGISTGFFDLQPKEGLALVNGTAVG 268

Query: 272 SAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFLL 331
           S +A+ V F+AN+ A+LA VLSA+F E M GKPEF D LTH+LKHHPGQIE+AA ME +L
Sbjct: 269 SGMASMVLFEANVQAVLAEVLSAIFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEHIL 328

Query: 332 DQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDNP 391
           D S +MK A+  HE DPL KPKQDRYALRTSPQWLGPQIEVIR AT SIEREINSVNDNP
Sbjct: 329 DGSSYMKLAQKVHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRQATKSIEREINSVNDNP 388

Query: 392 LIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLSG 451
           LIDV R+ A+HGGNFQGTPIGVSMDN R+AIAAIGKLMFAQFSELV D YNNGLPSNL+ 
Sbjct: 389 LIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLTA 448

Query: 452 GPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAI 511
             NPSLDYGFKGAEIAMA+YCSELQYLANPVT+HVQSAEQHNQDVNSLGLIS+RK++EA+
Sbjct: 449 SSNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEAV 508

Query: 512 EILKLMSATYMVALCQAIDLRQHEENMREVVKNALLQAVRKTLYVAEDGSLLESRFCEKD 571
           +ILKLMS T++V +CQA+DLR  EEN+R+ VKN + Q  +K L    +G L  SRFCEKD
Sbjct: 509 DILKLMSTTFLVGICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGINGELHPSRFCEKD 568

Query: 572 LLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERALKGASADKGEHGNETGVTIFRRIP 631
           LL+V++ E VF+Y+DDP + +Y L+ +L +V+V+ AL       GE       +IF++I 
Sbjct: 569 LLKVVDREQVFTYVDDPCSATYPLMQRLRQVIVDHAL-----SNGETEKNAVTSIFQKIG 628

Query: 632 GFLEELKAKLEEEVESARGRFENGDFAVENRIKKCSTYPIYHFVRTEVGTQLLSGAKRVS 691
            F EELKA L +EVE+AR  + NG   + NRIK+C +YP+Y FVR E+GT+LL+G K VS
Sbjct: 629 AFEEELKAVLPKEVEAARAAYGNGTAPIPNRIKECRSYPLYRFVREELGTKLLTGEKVVS 688

Query: 692 PGEDIEKVVDAINGGKIGEVVTKCLK 711
           PGE+ +KV  A+  GK+ + +  CLK
Sbjct: 689 PGEEFDKVFTAMCEGKLIDPLMDCLK 707

BLAST of MS028604 vs. TAIR 10
Match: AT2G37040.1 (PHE ammonia lyase 1 )

HSP 1 Score: 820.8 bits (2119), Expect = 8.2e-238
Identity = 434/692 (62.72%), Postives = 535/692 (77.31%), Query Frame = 0

Query: 27  VSASD--HWVKAADALRCPHFEEVRRMVSEFQEAKTVDIEGTTLTVAQVTAIAR-RSDVK 86
           ++A D  +W  AA+ ++  H +EV+RMV+EF++   V++ G TLT+ QV AI+   + VK
Sbjct: 30  INAEDPLNWGAAAEQMKGSHLDEVKRMVAEFRK-PVVNLGGETLTIGQVAAISTIGNSVK 89

Query: 87  VNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNA 146
           V L+E  AR  V  S++WV E++++GTD+YGVTTGFGATSHRRT     LQ ELIRFLNA
Sbjct: 90  VELSE-TARAGVNASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGVALQKELIRFLNA 149

Query: 147 GVIG-----KDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNENLIPKLPLRG 206
           G+ G        LP S ++AAMLVR NTL+QG+SGIR+EILEA+   +N N+ P LPLRG
Sbjct: 150 GIFGSTKETSHTLPHSATRAAMLVRINTLLQGFSGIRFEILEAITSFLNNNITPSLPLRG 209

Query: 207 TITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKRAGV-AGPFELQAKEGLAL 266
           TITASGDLVPLSYIAG++TGR NSK T   GE +TA +A K AG+ +G F+LQ KEGLAL
Sbjct: 210 TITASGDLVPLSYIAGLLTGRPNSKATGPNGEALTAEEAFKLAGISSGFFDLQPKEGLAL 269

Query: 267 VNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFADPLTHKLKHHPGQIESA 326
           VNGTAVGS +A+ V F+ N+L++LA +LSA+F E M GKPEF D LTH+LKHHPGQIE+A
Sbjct: 270 VNGTAVGSGMASMVLFETNVLSVLAEILSAVFAEVMSGKPEFTDHLTHRLKHHPGQIEAA 329

Query: 327 AAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREI 386
           A ME +LD S +MK A+  HE DPL KPKQDRYALRTSPQWLGPQIEVIR AT SIEREI
Sbjct: 330 AIMEHILDGSSYMKLAQKLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRYATKSIEREI 389

Query: 387 NSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNG 446
           NSVNDNPLIDV R+ A+HGGNFQGTPIGVSMDN R+AIAAIGKLMFAQFSELV D YNNG
Sbjct: 390 NSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNG 449

Query: 447 LPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISA 506
           LPSNL+   NPSLDYGFKGAEIAMA+YCSELQYLANPVT+HVQSAEQHNQDVNSLGLIS+
Sbjct: 450 LPSNLTASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISS 509

Query: 507 RKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALLQAVRKTLYVAEDGSLLE 566
           RK++EA++ILKLMS T++VA+CQA+DLR  EEN+R+ VKN + Q  +K L    +G L  
Sbjct: 510 RKTSEAVDILKLMSTTFLVAICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGVNGELHP 569

Query: 567 SRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERALKGASADKGEHGNETGV 626
           SRFCEKDLL+V++ E V++Y DDP + +Y LI KL +V+V+ AL       GE       
Sbjct: 570 SRFCEKDLLKVVDREQVYTYADDPCSATYPLIQKLRQVIVDHAL-----INGESEKNAVT 629

Query: 627 TIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCSTYPIYHFVRTEVGTQLL 686
           +IF +I  F EELKA L +EVE+AR  ++NG  A+ NRIK+C +YP+Y FVR E+GT+LL
Sbjct: 630 SIFHKIGAFEEELKAVLPKEVEAARAAYDNGTSAIPNRIKECRSYPLYRFVREELGTELL 689

Query: 687 SGAKRVSPGEDIEKVVDAINGGKIGEVVTKCL 710
           +G K  SPGE+ +KV  AI  GKI + + +CL
Sbjct: 690 TGEKVTSPGEEFDKVFTAICEGKIIDPMMECL 714

BLAST of MS028604 vs. TAIR 10
Match: AT3G10340.1 (phenylalanine ammonia-lyase 4 )

HSP 1 Score: 817.0 bits (2109), Expect = 1.2e-236
Identity = 435/699 (62.23%), Postives = 528/699 (75.54%), Query Frame = 0

Query: 19  HQSKSFPSVSASD-HWVKAADALRCPHFEEVRRMVSEFQEAKTVDIEGTTLTVAQVTAIA 78
           +Q+    +VS    +W   A+AL+  H +EV+RMV E+++ + V + G TLT+ QV A+A
Sbjct: 5   NQNNHITAVSGDPLNWNATAEALKGSHLDEVKRMVKEYRK-EAVKLGGETLTIGQVAAVA 64

Query: 79  RRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFGATSHRRTTKTADLQTEL 138
           R            AR  V  S+ WV E+++RGTD+YGVTTGFGATSHRRT +   LQ EL
Sbjct: 65  RGGGGSTVELAEEARAGVKASSEWVMESMNRGTDSYGVTTGFGATSHRRTKQGGALQNEL 124

Query: 139 IRFLNAGVIG------KDNLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNENLI 198
           IRFLNAG+ G         LP   ++AAMLVR NTL+QGYSGIR+EILEA+ KL+N  + 
Sbjct: 125 IRFLNAGIFGPGAGDTSHTLPKPTTRAAMLVRVNTLLQGYSGIRFEILEAITKLLNHEIT 184

Query: 199 PKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKRAGVAGPFELQA 258
           P LPLRGTITASGDLVPLSYIAG++TGR NSK     GE +TA +A K AGV+  FELQ 
Sbjct: 185 PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPSGETLTASEAFKLAGVSSFFELQP 244

Query: 259 KEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFADPLTHKLKHHP 318
           KEGLALVNGTAVGS +A+TV FDANILA+L+ V+SAMF E M GKPEF D LTHKLKHHP
Sbjct: 245 KEGLALVNGTAVGSGLASTVLFDANILAVLSEVMSAMFAEVMQGKPEFTDHLTHKLKHHP 304

Query: 319 GQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLGPQIEVIRTATH 378
           GQIE+AA ME +LD S ++KEA+  HE DPL KPKQDRYALRTSPQWLGPQIEVIR AT 
Sbjct: 305 GQIEAAAIMEHILDGSSYVKEAQLLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRAATK 364

Query: 379 SIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVC 438
            IEREINSVNDNPLIDV R+ ALHGGNFQGTPIGV+MDN R+AIA+IGKLMFAQFSELV 
Sbjct: 365 MIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVAMDNSRLAIASIGKLMFAQFSELVN 424

Query: 439 DHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNS 498
           D YNNGLPSNLSGG NPSLDYGFKGAEIAMA+YCSELQ+LANPVT HVQSAEQHNQDVNS
Sbjct: 425 DFYNNGLPSNLSGGRNPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNS 484

Query: 499 LGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALLQAVRKTLYVAE 558
           LGLIS+RK+AEA++ILKLMS TY+VALCQA+DLR  EEN+++ VK+A+ Q  ++ L V  
Sbjct: 485 LGLISSRKTAEAVDILKLMSTTYLVALCQAVDLRHLEENLKKAVKSAVSQVAKRVLTVGA 544

Query: 559 DGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERALKGASADKGEH 618
           +G L  SRF E+D+LQV++ E VFSY DDP + +Y L+ KL  +LV+ AL    AD    
Sbjct: 545 NGELHPSRFTERDVLQVVDREYVFSYADDPCSLTYPLMQKLRHILVDHAL----ADPERE 604

Query: 619 GNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCSTYPIYHFVRTE 678
            N +  ++F +I  F  ELK  L +EVE  R  +E G  A+ NRIK+C +YP+Y FVR E
Sbjct: 605 AN-SATSVFHKIGAFEAELKLLLPKEVERVRVEYEEGTSAIANRIKECRSYPLYRFVRDE 664

Query: 679 VGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLK 711
           + T+LL+G    SPGE+ +KV  AI+ GK+ + + +CLK
Sbjct: 665 LNTELLTGENVRSPGEEFDKVFLAISDGKLIDPLLECLK 697

BLAST of MS028604 vs. TAIR 10
Match: AT5G04230.1 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 750.0 bits (1935), Expect = 1.8e-216
Identity = 409/687 (59.53%), Postives = 503/687 (73.22%), Query Frame = 0

Query: 32  HWVKAADALRCPHFEEVRRMVSEFQEAKTVDIEGTTLTVAQVTAIARRSDVKVNLNEAVA 91
           +W  AA+AL+  H EEV++MV ++++  TV + G TLT+ QV A+A      V L+E  A
Sbjct: 16  NWNVAAEALKGSHLEEVKKMVKDYRKG-TVQLGGETLTIGQVAAVASGGPT-VELSEE-A 75

Query: 92  RDRVAKSANWVAENISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGKDN- 151
           R  V  S++WV E+++R TDTYG+TTGFG++S RRT + A LQ ELIR+LNAG+    N 
Sbjct: 76  RGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGIFATGNE 135

Query: 152 -------LPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNENLIPKLPLRGTITAS 211
                  LP   ++AAML+R NTL+QGYSGIR+EILEA+  L+N  + P LPLRGTITAS
Sbjct: 136 DDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKITPLLPLRGTITAS 195

Query: 212 GDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKRAGVAGPFELQAKEGLALVNGTAV 271
           GDLVPLSYIAG + GR NS+     GE +TA++A K AGV+  FEL+ KEGLALVNGTAV
Sbjct: 196 GDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGVSSFFELRPKEGLALVNGTAV 255

Query: 272 GSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFL 331
           GSA+A+TV +DANIL + + V SAMF E M GKPEF D LTHKLKHHPGQIE+AA ME +
Sbjct: 256 GSALASTVLYDANILVVFSEVASAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHI 315

Query: 332 LDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDN 391
           LD S ++KEA   H+ DPL KPKQDRYALRTSPQWLGPQIEVIR AT  IEREINSVNDN
Sbjct: 316 LDGSSYVKEALHLHKIDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIEREINSVNDN 375

Query: 392 PLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLS 451
           PLIDV R+ A+HGGNFQGTPIGV+MDN R+A+A+IGKLMFAQF+ELV D YNNGLPSNLS
Sbjct: 376 PLIDVSRNKAIHGGNFQGTPIGVAMDNTRLALASIGKLMFAQFTELVNDFYNNGLPSNLS 435

Query: 452 GGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEA 511
           GG NPSLDYG KGAE+AMA+YCSELQ+LANPVT HV+SA QHNQDVNSLGLIS+R +AEA
Sbjct: 436 GGRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQDVNSLGLISSRTTAEA 495

Query: 512 IEILKLMSATYMVALCQAIDLRQHEENMREVVKNALLQAVRKTLYVAEDGSLLESRFCEK 571
           + ILKLMS TY+VALCQA DLR  EE +++ V   +    +  L        +E      
Sbjct: 496 VVILKLMSTTYLVALCQAFDLRHLEEILKKAVNEVVSHTAKSVL-------AIEPFRKHD 555

Query: 572 DLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERALKGASADKGEHGNETGVTIFRRI 631
           D+L V+  E VFSY+DDP++ +  L+ KL  VL ++AL        E   ET  T+FR+I
Sbjct: 556 DILGVVNREYVFSYVDDPSSLTNPLMQKLRHVLFDKAL-------AEPEGETD-TVFRKI 615

Query: 632 PGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCSTYPIYHFVRTEVGTQLLSGAKRV 691
             F  ELK  L +EVE  R  +ENG F V NRIKKC +YP+Y FVR E+ T+LL+G    
Sbjct: 616 GAFEAELKFLLPKEVERVRTEYENGTFNVANRIKKCRSYPLYRFVRNELETRLLTGEDVR 675

Query: 692 SPGEDIEKVVDAINGGKIGEVVTKCLK 711
           SPGED +KV  AI+ GK+ + + +CLK
Sbjct: 676 SPGEDFDKVFRAISQGKLIDPLFECLK 684

BLAST of MS028604 vs. TAIR 10
Match: AT5G04230.2 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 744.2 bits (1920), Expect = 9.7e-215
Identity = 409/691 (59.19%), Postives = 503/691 (72.79%), Query Frame = 0

Query: 32  HWVKAADALRCPHFEEVRRMVSEFQEAKTVDIEGTTLTVAQVTAIARRSDVKVNLNEAVA 91
           +W  AA+AL+  H EEV++MV ++++  TV + G TLT+ QV A+A      V L+E  A
Sbjct: 16  NWNVAAEALKGSHLEEVKKMVKDYRKG-TVQLGGETLTIGQVAAVASGGPT-VELSEE-A 75

Query: 92  RDRVAKSANWVAENISRGTDTYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGKDN- 151
           R  V  S++WV E+++R TDTYG+TTGFG++S RRT + A LQ ELIR+LNAG+    N 
Sbjct: 76  RGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGIFATGNE 135

Query: 152 -------LPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNENLIPKLPLRGTITAS 211
                  LP   ++AAML+R NTL+QGYSGIR+EILEA+  L+N  + P LPLRGTITAS
Sbjct: 136 DDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKITPLLPLRGTITAS 195

Query: 212 GDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKRAGVAGPFELQAKEGLALVNGTAV 271
           GDLVPLSYIAG + GR NS+     GE +TA++A K AGV+  FEL+ KEGLALVNGTAV
Sbjct: 196 GDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGVSSFFELRPKEGLALVNGTAV 255

Query: 272 GSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFL 331
           GSA+A+TV +DANIL + + V SAMF E M GKPEF D LTHKLKHHPGQIE+AA ME +
Sbjct: 256 GSALASTVLYDANILVVFSEVASAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHI 315

Query: 332 LDQSDFMKEAKTRHEKDPLTKPKQDR----YALRTSPQWLGPQIEVIRTATHSIEREINS 391
           LD S ++KEA   H+ DPL KPKQDR    YALRTSPQWLGPQIEVIR AT  IEREINS
Sbjct: 316 LDGSSYVKEALHLHKIDPLQKPKQDRYVLGYALRTSPQWLGPQIEVIRAATKMIEREINS 375

Query: 392 VNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLP 451
           VNDNPLIDV R+ A+HGGNFQGTPIGV+MDN R+A+A+IGKLMFAQF+ELV D YNNGLP
Sbjct: 376 VNDNPLIDVSRNKAIHGGNFQGTPIGVAMDNTRLALASIGKLMFAQFTELVNDFYNNGLP 435

Query: 452 SNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARK 511
           SNLSGG NPSLDYG KGAE+AMA+YCSELQ+LANPVT HV+SA QHNQDVNSLGLIS+R 
Sbjct: 436 SNLSGGRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQDVNSLGLISSRT 495

Query: 512 SAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALLQAVRKTLYVAEDGSLLESR 571
           +AEA+ ILKLMS TY+VALCQA DLR  EE +++ V   +    +  L        +E  
Sbjct: 496 TAEAVVILKLMSTTYLVALCQAFDLRHLEEILKKAVNEVVSHTAKSVL-------AIEPF 555

Query: 572 FCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERALKGASADKGEHGNETGVTI 631
               D+L V+  E VFSY+DDP++ +  L+ KL  VL ++AL        E   ET  T+
Sbjct: 556 RKHDDILGVVNREYVFSYVDDPSSLTNPLMQKLRHVLFDKAL-------AEPEGETD-TV 615

Query: 632 FRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCSTYPIYHFVRTEVGTQLLSG 691
           FR+I  F  ELK  L +EVE  R  +ENG F V NRIKKC +YP+Y FVR E+ T+LL+G
Sbjct: 616 FRKIGAFEAELKFLLPKEVERVRTEYENGTFNVANRIKKCRSYPLYRFVRNELETRLLTG 675

Query: 692 AKRVSPGEDIEKVVDAINGGKIGEVVTKCLK 711
               SPGED +KV  AI+ GK+ + + +CLK
Sbjct: 676 EDVRSPGEDFDKVFRAISQGKLIDPLFECLK 688

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147741.10.0e+0099.86phenylalanine ammonia-lyase-like [Momordica charantia][more]
XP_038879437.10.0e+0088.33phenylalanine ammonia-lyase-like [Benincasa hispida][more]
XP_023516501.10.0e+0088.19phenylalanine ammonia-lyase-like [Cucurbita pepo subsp. pepo] >AXF54191.1 PAL1 [... [more]
KAG6590021.10.0e+0088.19Phenylalanine ammonia-lyase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022961017.10.0e+0087.90phenylalanine ammonia-lyase-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P527778.8e-25365.36Phenylalanine ammonia-lyase OS=Pinus taeda OX=3352 GN=PAL PE=3 SV=1[more]
A0A2H5AIW06.5e-24063.56Phenylalanine ammonia-lyase 1 OS=Narcissus pseudonarcissus OX=39639 GN=PAL1 PE=2... [more]
Q50EX78.5e-24064.34Phenylalanine ammonia-lyase 1 OS=Petunia hybrida OX=4102 GN=PAL1 PE=1 SV=1[more]
P457264.2e-23963.99Phenylalanine ammonia-lyase OS=Camellia sinensis OX=4442 GN=PAL PE=2 SV=1[more]
O238655.5e-23964.29Phenylalanine ammonia-lyase 1 OS=Daucus carota OX=4039 GN=PAL1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D3950.0e+0099.86Phenylalanine ammonia-lyase OS=Momordica charantia OX=3673 GN=LOC111016602 PE=3 ... [more]
A0A345BTD40.0e+0088.19Phenylalanine ammonia-lyase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
A0A6J1H8Z90.0e+0087.90Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111461649 PE=3 S... [more]
A0A6J1JJE50.0e+0087.90Phenylalanine ammonia-lyase OS=Cucurbita maxima OX=3661 GN=LOC111485128 PE=3 SV=... [more]
A0A0A0LYZ40.0e+0085.94Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_1G590300 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G53260.14.8e-23862.83phenylalanine ammonia-lyase 2 [more]
AT2G37040.18.2e-23862.72PHE ammonia lyase 1 [more]
AT3G10340.11.2e-23662.23phenylalanine ammonia-lyase 4 [more]
AT5G04230.11.8e-21659.53phenyl alanine ammonia-lyase 3 [more]
AT5G04230.29.7e-21559.19phenyl alanine ammonia-lyase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023144Phenylalanine ammonia-lyase, shielding domain superfamilyGENE3D1.10.274.20coord: 528..654
e-value: 4.9E-215
score: 716.5
IPR005922Phenylalanine ammonia-lyaseTIGRFAMTIGR01226TIGR01226coord: 31..710
e-value: 1.6E-302
score: 1002.9
IPR001106Aromatic amino acid lyasePFAMPF00221Lyase_aromaticcoord: 68..544
e-value: 6.4E-155
score: 516.3
IPR001106Aromatic amino acid lyasePANTHERPTHR10362HISTIDINE AMMONIA-LYASEcoord: 35..710
IPR001106Aromatic amino acid lyaseCDDcd00332PAL-HALcoord: 66..525
e-value: 0.0
score: 535.557
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 262..710
e-value: 4.9E-215
score: 716.5
NoneNo IPR availablePANTHERPTHR10362:SF58PHENYLALANINE AMMONIA-LYASEcoord: 35..710
IPR024083Fumarase/histidase, N-terminalGENE3D1.10.275.10coord: 22..261
e-value: 1.6E-81
score: 275.3
IPR022313Phenylalanine/histidine ammonia-lyases, active sitePROSITEPS00488PAL_HISTIDASEcoord: 198..214
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 32..710

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS028604.1MS028604.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006559 L-phenylalanine catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016841 ammonia-lyase activity
molecular_function GO:0003824 catalytic activity