MS028584 (gene) Bitter gourd (TR) v1

Overview


Name	MS028584
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	patatin-like protein 2
Location	scaffold357: 305277 .. 305615 (+)
RNA-Seq Expression	MS028584
Synteny	MS028584

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTTGTTATCCTTCACAAAATGGGGATGTACAACTGGGCATCCTTTTTCGAGGTACTGAATCCGAAGCCTATTATCTTCGTATTCACGAAGAAGTGCCCAGTGAAATCCTGGCATCAGCATCAGTAGACATGGCCACTACCAAGAATCTTAAGCGACTCGTAAAATTCGGTGAACAATTGTTGGACAAGCCTGTCTCCAAGGAGGGGATGCTTTATATAGAGGATGCAGTGCACAAGGGTTACCCCTCTGAAACCAATCGCCAAGCTTTGGTCAGGTTCGCCAAATTGCTCTCACACAACAAAAGGTCTTCTTCTACCAACAACACCAGAACTTGA

mRNA sequence

ATGTTTTGTTATCCTTCACAAAATGGGGATGTACAACTGGGCATCCTTTTTCGAGGTACTGAATCCGAAGCCTATTATCTTCGTATTCACGAAGAAGTGCCCAGTGAAATCCTGGCATCAGCATCAGTAGACATGGCCACTACCAAGAATCTTAAGCGACTCGTAAAATTCGGTGAACAATTGTTGGACAAGCCTGTCTCCAAGGAGGGGATGCTTTATATAGAGGATGCAGTGCACAAGGGTTACCCCTCTGAAACCAATCGCCAAGCTTTGGTCAGGTTCGCCAAATTGCTCTCACACAACAAAAGGTCTTCTTCTACCAACAACACCAGAACTTGA

Coding sequence (CDS)

ATGTTTTGTTATCCTTCACAAAATGGGGATGTACAACTGGGCATCCTTTTTCGAGGTACTGAATCCGAAGCCTATTATCTTCGTATTCACGAAGAAGTGCCCAGTGAAATCCTGGCATCAGCATCAGTAGACATGGCCACTACCAAGAATCTTAAGCGACTCGTAAAATTCGGTGAACAATTGTTGGACAAGCCTGTCTCCAAGGAGGGGATGCTTTATATAGAGGATGCAGTGCACAAGGGTTACCCCTCTGAAACCAATCGCCAAGCTTTGGTCAGGTTCGCCAAATTGCTCTCACACAACAAAAGGTCTTCTTCTACCAACAACACCAGAACTTGA

Protein sequence

MFCYPSQNGDVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKEGMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTRT

Homology

[Close]

BLAST of MS028584 vs. NCBI nr
Match: XP_022159387.1 (patatin-like protein 2 [Momordica charantia])

HSP 1 Score: 211.8 bits (538), Expect = 2.9e-51
Identity = 105/112 (93.75%), Postives = 109/112 (97.32%), Query Frame = 0


Query: 1   MFCYPSQNGDVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQ 60
           MFC+PSQNGDV LGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQ
Sbjct: 92  MFCHPSQNGDVNLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQ 151

Query: 61  LLDKPVSKEGMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTRT 113
           LLDKPVSKEG+ Y++DAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNT T
Sbjct: 152 LLDKPVSKEGIFYMKDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTTT 203

[Close]

BLAST of MS028584 vs. NCBI nr
Match: KAB1220376.1 (Patatin-like protein 2 [Morella rubra])

HSP 1 Score: 78.2 bits (191), Expect = 5.1e-11
Identity = 45/94 (47.87%), Postives = 63/94 (67.02%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 69
           DV L ++F+   SE  YLRI ++  +  +  +SVD+ATTKNL  LVK GE+LL KPV++ 
Sbjct: 303 DVHLSVVFQALHSEKSYLRIQDDTLTGTV--SSVDVATTKNLDDLVKVGEELLKKPVAR- 362

Query: 70  GMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKR 104
             + +E   ++   SETN +ALVRFAKLLS  +R
Sbjct: 363 --VNLETGKYEASNSETNAEALVRFAKLLSQERR 391

[Close]

BLAST of MS028584 vs. NCBI nr
Match: XP_040997731.1 (patatin-like protein 2 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 76.6 bits (187), Expect = 1.5e-10
Identity = 49/100 (49.00%), Postives = 65/100 (65.00%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 69
           DV L  +FR   SE  YLRI ++  S ++  +SVD+AT+KNL  LVK GE+LL KPV++ 
Sbjct: 303 DVHLSQVFRALHSEKSYLRIQDDTLSGVV--SSVDVATSKNLDDLVKVGEELLRKPVAR- 362

Query: 70  GMLYIEDAVHKGYPSETNRQALVRFAKLLS---HNKRSSS 107
             + +E  V++   SETN QAL RFAKLLS   H + S S
Sbjct: 363 --VNLETGVYEPSNSETNEQALRRFAKLLSQERHRRHSRS 397

[Close]

BLAST of MS028584 vs. NCBI nr
Match: KAG8632591.1 (hypothetical protein MANES_18G033422v8 [Manihot esculenta])

HSP 1 Score: 76.6 bits (187), Expect = 1.5e-10
Identity = 43/94 (45.74%), Postives = 61/94 (64.89%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 69
           D  + ++F+   SE+ YLRI ++  S  +  +SVD+AT KNL  LVK GE LL KPVS+ 
Sbjct: 306 DFHISVVFKALHSESNYLRIQDDTLSRTV--SSVDVATKKNLDDLVKVGEGLLKKPVSR- 365

Query: 70  GMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKR 104
             + +E  V + +  ETN +AL+RFAKLLS  +R
Sbjct: 366 --VNLETGVFEPFGKETNEEALIRFAKLLSEERR 394

[Close]

BLAST of MS028584 vs. NCBI nr
Match: KAG5251292.1 (patatin family protein [Salix suchowensis])

HSP 1 Score: 76.3 bits (186), Expect = 1.9e-10
Identity = 45/94 (47.87%), Postives = 61/94 (64.89%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 69
           D  LG +F+   SE  YLRI E+  +  L  ASVD+AT KNLK LVK G++LL KPVS+ 
Sbjct: 302 DFHLGTVFQALRSEDNYLRIQEDTLTGTL--ASVDIATKKNLKNLVKVGKELLKKPVSR- 361

Query: 70  GMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKR 104
             + +E  V++     TN +ALV+FAK+LS  K+
Sbjct: 362 --VNLETGVYEPVDKLTNEEALVKFAKILSRQKQ 390

[Close]

BLAST of MS028584 vs. ExPASy Swiss-Prot
Match: O48723 (Patatin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PLP2 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 7.1e-08
Identity = 34/94 (36.17%), Postives = 55/94 (58.51%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 69
           D  L  +FR   SEA Y+RI ++  +    +ASVD+AT +NL  L K G++LL KPV++ 
Sbjct: 304 DFHLSAVFRALHSEANYIRIQDDTLTG--DAASVDIATVENLDILAKTGDELLKKPVAR- 363

Query: 70  GMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKR 104
             + ++   ++     TN  AL++ A +LS  K+
Sbjct: 364 --VNLDSGCNENAYETTNEHALIKLAGILSKEKK 392

[Close]

BLAST of MS028584 vs. ExPASy Swiss-Prot
Match: A2YW91 (Patatin-like protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=PLP2 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 3.5e-07
Identity = 38/97 (39.18%), Postives = 54/97 (55.67%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASA-SVDMATTKNLKRLVKFGEQLLDKPVSK 69
           D+ LG+LF   + E  YLRI  +   ++  SA S+D  + +N+  LVK GE LLDK VS+
Sbjct: 308 DIHLGVLFSALQCEKNYLRIQYD---QLTGSAGSIDDCSKENMDNLVKIGEMLLDKNVSR 367

Query: 70  EGML--YIEDAVHKGYPSETNRQALVRFAKLLSHNKR 104
             +   +  D   +G    TNR  L +FAK LS  +R
Sbjct: 368 VDLETGHYVDVAGEG----TNRDQLAKFAKQLSDERR 397

[Close]

BLAST of MS028584 vs. ExPASy Swiss-Prot
Match: Q6ZJD3 (Patatin-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PLP2 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 3.5e-07
Identity = 38/97 (39.18%), Postives = 54/97 (55.67%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASA-SVDMATTKNLKRLVKFGEQLLDKPVSK 69
           D+ LG+LF   + E  YLRI  +   ++  SA S+D  + +N+  LVK GE LLDK VS+
Sbjct: 308 DIHLGVLFSALQCEKNYLRIQYD---QLTGSAGSIDDCSKENMDNLVKIGEMLLDKNVSR 367

Query: 70  EGML--YIEDAVHKGYPSETNRQALVRFAKLLSHNKR 104
             +   +  D   +G    TNR  L +FAK LS  +R
Sbjct: 368 VDLETGHYVDVAGEG----TNRDQLAKFAKQLSDERR 397

[Close]

BLAST of MS028584 vs. ExPASy Swiss-Prot
Match: O23181 (Patatin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PLP3 PE=2 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 7.9e-07
Identity = 38/96 (39.58%), Postives = 56/96 (58.33%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 69
           D Q  ++F+   SE  YLRI ++     L   SVD++T KN++ LV+ GE LL K VS+ 
Sbjct: 328 DYQSSVVFQALRSEKNYLRIDDDSLKGDL--GSVDISTEKNMEGLVEVGEALLKKRVSRV 387

Query: 70  GMLYIEDAVHKGYPSE--TNRQALVRFAKLLSHNKR 104
            +    ++ H    SE  TN +AL RFAK+LS  ++
Sbjct: 388 NL----ESGHYQPISENVTNEEALKRFAKVLSEERK 417

[Close]

BLAST of MS028584 vs. ExPASy Swiss-Prot
Match: Q2MY43 (Patatin-08 OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 6.7e-06
Identity = 34/94 (36.17%), Postives = 48/94 (51.06%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 69
           D  L  +F+   S+  YLR+ E   +    +  +D A+  N++ LV+ GE LL KPVSK+
Sbjct: 301 DYYLSTVFQAQNSQKNYLRVQENALTG--TTTEMDDASEANMESLVQVGENLLKKPVSKD 360

Query: 70  GMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKR 104
                          ET  +AL RFAKLLS  K+
Sbjct: 361 N-------------PETYEEALKRFAKLLSDRKK 379

[Close]

BLAST of MS028584 vs. ExPASy TrEMBL
Match: A0A6J1DZQ3 (patatin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025803 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.4e-51
Identity = 105/112 (93.75%), Postives = 109/112 (97.32%), Query Frame = 0


Query: 1   MFCYPSQNGDVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQ 60
           MFC+PSQNGDV LGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQ
Sbjct: 92  MFCHPSQNGDVNLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQ 151

Query: 61  LLDKPVSKEGMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTRT 113
           LLDKPVSKEG+ Y++DAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNT T
Sbjct: 152 LLDKPVSKEGIFYMKDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTTT 203

[Close]

BLAST of MS028584 vs. ExPASy TrEMBL
Match: A0A6A1W7T7 (Patatin OS=Morella rubra OX=262757 GN=CJ030_MR3G009836 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 2.5e-11
Identity = 45/94 (47.87%), Postives = 63/94 (67.02%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 69
           DV L ++F+   SE  YLRI ++  +  +  +SVD+ATTKNL  LVK GE+LL KPV++ 
Sbjct: 303 DVHLSVVFQALHSEKSYLRIQDDTLTGTV--SSVDVATTKNLDDLVKVGEELLKKPVAR- 362

Query: 70  GMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKR 104
             + +E   ++   SETN +ALVRFAKLLS  +R
Sbjct: 363 --VNLETGKYEASNSETNAEALVRFAKLLSQERR 391

[Close]

BLAST of MS028584 vs. ExPASy TrEMBL
Match: A0A2I4E9L9 (Patatin OS=Juglans regia OX=51240 GN=LOC108987613 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.6e-10
Identity = 47/112 (41.96%), Postives = 69/112 (61.61%), Query Frame = 0


Query: 1   MFCYPSQN-GDVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGE 60
           +FC  S +  DV L  +F+   SE  YLRI ++  + ++  +SVD+ATTKNL  LVK GE
Sbjct: 294 VFCQASADMVDVHLSQVFQDLHSEKSYLRIQDDTLTGVV--SSVDVATTKNLNDLVKVGE 353

Query: 61  QLLDKPVSKEGMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNNTR 112
           +LL KPV++   + +E  + +     TN QAL+RFAKLLS  +R   +  +R
Sbjct: 354 ELLKKPVAR---VNLETGICEPVDIGTNEQALIRFAKLLSQERRLRHSRTSR 400

[Close]

BLAST of MS028584 vs. ExPASy TrEMBL
Match: B9R7G7 (Patatin OS=Ricinus communis OX=3988 GN=RCOM_1591480 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.1e-10
Identity = 45/100 (45.00%), Postives = 63/100 (63.00%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 69
           D+ +  +F+   SE+ YLRI +E  S+I+  +SVD+AT KNL  LVK GE LL KPVS+ 
Sbjct: 305 DLHISEVFQALRSESNYLRIQDETLSKIV--SSVDVATKKNLNDLVKVGEGLLKKPVSR- 364

Query: 70  GMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKRSSSTNN 110
             + +E  +      ETN +AL+RFAKLLS  +R    N+
Sbjct: 365 --VNLEGGIFAPCNKETNEEALIRFAKLLSEERRLRHINS 399

[Close]

BLAST of MS028584 vs. ExPASy TrEMBL
Match: B9R7G6 (Patatin OS=Ricinus communis OX=3988 GN=RCOM_1591470 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.7e-10
Identity = 43/94 (45.74%), Postives = 59/94 (62.77%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 69
           D+ + +LF+   SE  YLRI ++  SE +  ASVD+AT KNL  L K GE LL KPVS+ 
Sbjct: 302 DLHISVLFQALHSERNYLRIQDDTLSETV--ASVDVATKKNLNDLAKIGEGLLKKPVSR- 361

Query: 70  GMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKR 104
             + +E  + +    ETN +AL+RFAK LS  +R
Sbjct: 362 --VNLETGIFELVNEETNEEALIRFAKQLSEERR 390

[Close]

BLAST of MS028584 vs. TAIR 10
Match: AT2G26560.1 (phospholipase A 2A )

HSP 1 Score: 58.2 bits (139), Expect = 5.1e-09
Identity = 34/94 (36.17%), Postives = 55/94 (58.51%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 69
           D  L  +FR   SEA Y+RI ++  +    +ASVD+AT +NL  L K G++LL KPV++ 
Sbjct: 304 DFHLSAVFRALHSEANYIRIQDDTLTG--DAASVDIATVENLDILAKTGDELLKKPVAR- 363

Query: 70  GMLYIEDAVHKGYPSETNRQALVRFAKLLSHNKR 104
             + ++   ++     TN  AL++ A +LS  K+
Sbjct: 364 --VNLDSGCNENAYETTNEHALIKLAGILSKEKK 392

[Close]

BLAST of MS028584 vs. TAIR 10
Match: AT4G37050.1 (PATATIN-like protein 4 )

HSP 1 Score: 54.7 bits (130), Expect = 5.6e-08
Identity = 38/96 (39.58%), Postives = 56/96 (58.33%), Query Frame = 0


Query: 10  DVQLGILFRGTESEAYYLRIHEEVPSEILASASVDMATTKNLKRLVKFGEQLLDKPVSKE 69
           D Q  ++F+   SE  YLRI ++     L   SVD++T KN++ LV+ GE LL K VS+ 
Sbjct: 328 DYQSSVVFQALRSEKNYLRIDDDSLKGDL--GSVDISTEKNMEGLVEVGEALLKKRVSRV 387

Query: 70  GMLYIEDAVHKGYPSE--TNRQALVRFAKLLSHNKR 104
            +    ++ H    SE  TN +AL RFAK+LS  ++
Sbjct: 388 NL----ESGHYQPISENVTNEEALKRFAKVLSEERK 417

The following BLAST results are available for this feature:

BLAST of MS028584 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159387.1	2.9e-51	93.75	patatin-like protein 2 [Momordica charantia]	[more]
KAB1220376.1	5.1e-11	47.87	Patatin-like protein 2 [Morella rubra]	[more]
XP_040997731.1	1.5e-10	49.00	patatin-like protein 2 [Juglans microcarpa x Juglans regia]	[more]
KAG8632591.1	1.5e-10	45.74	hypothetical protein MANES_18G033422v8 [Manihot esculenta]	[more]
KAG5251292.1	1.9e-10	47.87	patatin family protein [Salix suchowensis]	[more]

BLAST of MS028584 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48723	7.1e-08	36.17	Patatin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PLP2 PE=1 SV=1	[more]
A2YW91	3.5e-07	39.18	Patatin-like protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=PLP2 PE=3 SV=1	[more]
Q6ZJD3	3.5e-07	39.18	Patatin-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PLP2 PE=3 SV=...	[more]
O23181	7.9e-07	39.58	Patatin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PLP3 PE=2 SV=2	[more]
Q2MY43	6.7e-06	36.17	Patatin-08 OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]

BLAST of MS028584 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DZQ3	1.4e-51	93.75	patatin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025803 PE=4 SV=1	[more]
A0A6A1W7T7	2.5e-11	47.87	Patatin OS=Morella rubra OX=262757 GN=CJ030_MR3G009836 PE=3 SV=1	[more]
A0A2I4E9L9	1.6e-10	41.96	Patatin OS=Juglans regia OX=51240 GN=LOC108987613 PE=3 SV=1	[more]
B9R7G7	2.1e-10	45.00	Patatin OS=Ricinus communis OX=3988 GN=RCOM_1591480 PE=3 SV=1	[more]
B9R7G6	2.7e-10	45.74	Patatin OS=Ricinus communis OX=3988 GN=RCOM_1591470 PE=3 SV=1	[more]

BLAST of MS028584 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G26560.1	5.1e-09	36.17	phospholipase A 2A	[more]
AT4G37050.1	5.6e-08	39.58	PATATIN-like protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.1090.10	Cytosolic phospholipase A2 catalytic domain	coord: 3..111 e-value: 2.1E-10 score: 42.3
None	No IPR available	PANTHER	PTHR32176	XYLOSE ISOMERASE	coord: 10..105
None	No IPR available	PANTHER	PTHR32176:SF64	PATATIN-LIKE PROTEIN 3	coord: 10..105

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS028584.1	MS028584.1	mRNA