MS028333 (gene) Bitter gourd (TR) v1

Overview


Name	MS028333
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	BHLH domain-containing protein
Location	scaffold47: 3497629 .. 3499012 (+)
RNA-Seq Expression	MS028333
Synteny	MS028333

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCGAAATTTCAAGTTTTCTGCTGCTTCTTCCTCTTCCTCCAAGGTCGAACGCAGAATTGTGGAGAAAAACAGAAGAAATGAGATGAAAAACCTCTGCTCTCAGCTCAATTCCCTCCTCCCATATCAGGATTCAGAGGTCCTTTTTTTTACAATTTTTATGTGATTTCCGCAACAGGGTCATCTCTGTTCTACTCTGTTTTACTCTGTTTCTGCGTTTTGTGCAGAAATTTAGATGTTTTGTACATAAAATTTAGGTGTTATTTCATGCAGCAGATTTCACTGACACTGCCGGAGCAGATAGACGAAGCGATAAAGTACATAAAAGAGGCAGAGAAGAAAGTGAACGCTGCGAAGGAGAAGAAGGAGCGGCTGATGGGGAAGCAGAGCTCCACGGCCGCAACAATGGAGGGTTCTTCTTCTTCTTCTCCGGCGGCGCCTCAGCTGAAAATTCAGGAGATTGGTCGCAGTCTGCAGATTTTTCTGAGCAGTGGATCCGACAATCAGTTCCTCTTCCGCGAAACCATTCGCATTCTTCAACAAGAAGGTGCCGAAGTTGTCAGTGCCGGCTTCTCCGCCGCCGCCAACTCTGTTCTCCACACAATCCATGCCCAAGTAACCATATTATCAAAACAATAAACTTTATATTATAATTTTTTTAATCACCGGTGGACCCACACAACTCGGTTCAACTGGATGCAGCCATATGGTCCAACCGTTCAATAGGTTCATATCTATTATAAACCTTTGTTAAGGATAGGTCAGATCTCGGAATGGATACTCGTTTGAGCATTTTGGTTCTTTGATACCGTATCAATATTCATGGGTGTCATCTCAAGATTAATTGTTGAGGATTCTACGTTCAAAAGAAGATGGATCCTACAAAAGTTTATAACAGACAAGTATTATTCCTCTCATCTCATTGTATATTAATACGGTATCAAAGCTTGAAGCGTTGAAACAAGCATATGGTCAAAAAATGAGATCCGATCCAGATTGATGAGTCAAAAGAGCCATGAATTTGAAGGGATATGTTAAGGATGCTTTCAATAGAAAATGAATCTTACAAAGGTTTATAATAGATATAGACTAAATCTCTTATCGTGGAATCCTTAACATAACTTTGATTGGAGAGTCAAGTGTCTGCTCGACTTGCATCTTTGACCTCACTTCAAGTGGACCGAGTACAATAATTTCATCCCTAACATAACTTGTTTTGTTTTTTTGGGCAGCTTGGGGAATCCATGGTTGAATTTGGAGTGGCAAAAGTGAGCGAGAGACTGAAGAGTTTGGTTTATGGGTCGGGCAGCGACGTGGAGTTGCAGGAGGAGCAGCCATGGTGGGACTTCGAATTCCCACCTGACGCCACGTGGAATTTCTAA

mRNA sequence

ATGGATCGAAATTTCAAGTTTTCTGCTGCTTCTTCCTCTTCCTCCAAGGTCGAACGCAGAATTGTGGAGAAAAACAGAAGAAATGAGATGAAAAACCTCTGCTCTCAGCTCAATTCCCTCCTCCCATATCAGGATTCAGAGCAGATTTCACTGACACTGCCGGAGCAGATAGACGAAGCGATAAAGTACATAAAAGAGGCAGAGAAGAAAGTGAACGCTGCGAAGGAGAAGAAGGAGCGGCTGATGGGGAAGCAGAGCTCCACGGCCGCAACAATGGAGGGTTCTTCTTCTTCTTCTCCGGCGGCGCCTCAGCTGAAAATTCAGGAGATTGGTCGCAGTCTGCAGATTTTTCTGAGCAGTGGATCCGACAATCAGTTCCTCTTCCGCGAAACCATTCGCATTCTTCAACAAGAAGGTGCCGAAGTTGTCAGTGCCGGCTTCTCCGCCGCCGCCAACTCTGTTCTCCACACAATCCATGCCCAACTTGGGGAATCCATGGTTGAATTTGGAGTGGCAAAAGTGAGCGAGAGACTGAAGAGTTTGGTTTATGGGTCGGGCAGCGACGTGGAGTTGCAGGAGGAGCAGCCATGGTGGGACTTCGAATTCCCACCTGACGCCACGTGGAATTTCTAA

Coding sequence (CDS)

ATGGATCGAAATTTCAAGTTTTCTGCTGCTTCTTCCTCTTCCTCCAAGGTCGAACGCAGAATTGTGGAGAAAAACAGAAGAAATGAGATGAAAAACCTCTGCTCTCAGCTCAATTCCCTCCTCCCATATCAGGATTCAGAGCAGATTTCACTGACACTGCCGGAGCAGATAGACGAAGCGATAAAGTACATAAAAGAGGCAGAGAAGAAAGTGAACGCTGCGAAGGAGAAGAAGGAGCGGCTGATGGGGAAGCAGAGCTCCACGGCCGCAACAATGGAGGGTTCTTCTTCTTCTTCTCCGGCGGCGCCTCAGCTGAAAATTCAGGAGATTGGTCGCAGTCTGCAGATTTTTCTGAGCAGTGGATCCGACAATCAGTTCCTCTTCCGCGAAACCATTCGCATTCTTCAACAAGAAGGTGCCGAAGTTGTCAGTGCCGGCTTCTCCGCCGCCGCCAACTCTGTTCTCCACACAATCCATGCCCAACTTGGGGAATCCATGGTTGAATTTGGAGTGGCAAAAGTGAGCGAGAGACTGAAGAGTTTGGTTTATGGGTCGGGCAGCGACGTGGAGTTGCAGGAGGAGCAGCCATGGTGGGACTTCGAATTCCCACCTGACGCCACGTGGAATTTCTAA

Protein sequence

MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEAIKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSSGSDNQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKSLVYGSGSDVELQEEQPWWDFEFPPDATWNF

Homology

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BLAST of MS028333 vs. NCBI nr
Match: XP_022147998.1 (transcription factor bHLH168 isoform X1 [Momordica charantia])

HSP 1 Score: 377.5 bits (968), Expect = 7.6e-101
Identity = 204/211 (96.68%), Postives = 206/211 (97.63%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           MDRNFK SAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLP QDS+QISLTLPEQIDEA
Sbjct: 1   MDRNFKLSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPNQDSKQISLTLPEQIDEA 60

Query: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120
           IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS
Sbjct: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120

Query: 121 GSDNQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKS 180
           GSDNQFLFRETIRILQQEGAEVVSA FSAAANSVLHTIHAQLGESMVEFGVAKVSERLK 
Sbjct: 121 GSDNQFLFRETIRILQQEGAEVVSASFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKG 180

Query: 181 LVYGSGSDVELQ-EEQPWWDFEFPPDATWNF 211
           LVYGSGSDVELQ EEQPWW+FEFPPDATWNF
Sbjct: 181 LVYGSGSDVELQKEEQPWWEFEFPPDATWNF 211

[Close]

BLAST of MS028333 vs. NCBI nr
Match: XP_022147999.1 (transcription factor bHLH162 isoform X2 [Momordica charantia])

HSP 1 Score: 371.3 bits (952), Expect = 5.4e-99
Identity = 203/211 (96.21%), Postives = 205/211 (97.16%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           MDRNFK SAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLP QDS +ISLTLPEQIDEA
Sbjct: 1   MDRNFKLSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPNQDS-KISLTLPEQIDEA 60

Query: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120
           IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS
Sbjct: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120

Query: 121 GSDNQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKS 180
           GSDNQFLFRETIRILQQEGAEVVSA FSAAANSVLHTIHAQLGESMVEFGVAKVSERLK 
Sbjct: 121 GSDNQFLFRETIRILQQEGAEVVSASFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKG 180

Query: 181 LVYGSGSDVELQ-EEQPWWDFEFPPDATWNF 211
           LVYGSGSDVELQ EEQPWW+FEFPPDATWNF
Sbjct: 181 LVYGSGSDVELQKEEQPWWEFEFPPDATWNF 210

[Close]

BLAST of MS028333 vs. NCBI nr
Match: XP_038888628.1 (transcription factor bHLH162-like [Benincasa hispida])

HSP 1 Score: 266.5 bits (680), Expect = 1.9e-67
Identity = 141/203 (69.46%), Postives = 172/203 (84.73%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           MD NF+FSA+SSS++K+ERRI+EKNRRN+MKNLCSQLNSLLP  DS+ ISL LP+QIDEA
Sbjct: 1   MDPNFQFSASSSSAAKIERRIIEKNRRNQMKNLCSQLNSLLPNHDSKDISLPLPDQIDEA 60

Query: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120
           IKYIKE EKKV++AKEKK +L GK  S +  M+ SSSSS A+PQL I ++G+S++I LSS
Sbjct: 61  IKYIKELEKKVSSAKEKKNQLQGKNKS-SINMDSSSSSSSASPQLNIHQMGKSVEIVLSS 120

Query: 121 GSDNQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKS 180
           G DNQ+LF ET+RILQ+EG EV++A FS + NSV HTIHAQLG+SMVEFGV K +ERLK 
Sbjct: 121 GLDNQYLFCETLRILQEEGTEVINASFSVSGNSVFHTIHAQLGDSMVEFGVTKATERLKR 180

Query: 181 LVYGSGSDVELQEEQPWWDFEFP 204
           LVYGS SDVE+Q+E+ WWD EFP
Sbjct: 181 LVYGSNSDVEVQKEKQWWD-EFP 201

[Close]

BLAST of MS028333 vs. NCBI nr
Match: XP_022930718.1 (transcription factor bHLH162-like [Cucurbita moschata])

HSP 1 Score: 257.7 bits (657), Expect = 8.8e-65
Identity = 142/206 (68.93%), Postives = 168/206 (81.55%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           MDR+F+FS +SSS++K+ERRIVEKNRRN+MK LCSQLNSLLP QDS++ISL LP+QIDEA
Sbjct: 50  MDRHFQFSTSSSSAAKIERRIVEKNRRNQMKTLCSQLNSLLPNQDSKEISLALPDQIDEA 109

Query: 61  IKYIKEAEKKVNAAKEKKERLMGK-QSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLS 120
           +KYIKE EKKVN AKEKKE+L GK +SS       SSSSS A+PQLKI + GRSL+I LS
Sbjct: 110 VKYIKELEKKVNLAKEKKEKLEGKNKSSMNIESSSSSSSSSASPQLKIHQSGRSLEIILS 169

Query: 121 SGSDNQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLK 180
           SG DNQ+LF ETI ILQ+EG EVV+A FS + +SV  T HAQL  SM+EF  AKV+ERLK
Sbjct: 170 SGLDNQYLFSETISILQEEGVEVVNASFSVSGSSVFQTFHAQLEGSMIEFEAAKVTERLK 229

Query: 181 SLVYGSGSDVELQEEQPWWDFEFPPD 206
            LVYGS SD+ELQ+E+ WWD EFP D
Sbjct: 230 RLVYGSNSDMELQKEKQWWD-EFPSD 254

[Close]

BLAST of MS028333 vs. NCBI nr
Match: XP_023531689.1 (transcription factor bHLH162-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 256.1 bits (653), Expect = 2.6e-64
Identity = 142/205 (69.27%), Postives = 169/205 (82.44%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           MDR+F+FS +SSS++K+ERRIVEKNRRN+MK LCSQLNSLLP QDS++ISL LP+QIDEA
Sbjct: 46  MDRHFQFSTSSSSAAKIERRIVEKNRRNQMKTLCSQLNSLLPNQDSKEISLALPDQIDEA 105

Query: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120
           +KYIKE EKKVN AKEKKE+L GK  S +  +E SSSSS A+PQLKI + GRSL+I LSS
Sbjct: 106 VKYIKELEKKVNLAKEKKEKLEGKNKS-SMNIESSSSSS-ASPQLKIHQSGRSLEIILSS 165

Query: 121 GSDNQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKS 180
           G DNQ+LF ETI ILQ+EG EVV+A FS + +SV  T HAQL  SM+EF  AKV+ERLK 
Sbjct: 166 GLDNQYLFSETISILQEEGVEVVNASFSVSGSSVFQTFHAQLEGSMIEFEAAKVTERLKR 225

Query: 181 LVYGSGSDVELQEEQPWWDFEFPPD 206
           LVYGS SD+ELQ+E+ WWD EFP D
Sbjct: 226 LVYGSNSDMELQKEKQWWD-EFPSD 247

[Close]

BLAST of MS028333 vs. ExPASy Swiss-Prot
Match: F4JIJ7 (Transcription factor bHLH162 OS=Arabidopsis thaliana OX=3702 GN=BHLH162 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.2e-21
Identity = 70/182 (38.46%), Postives = 111/182 (60.99%), Query Frame = 0


Query: 11  SSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEAIKYIKEAEKK 70
           +  S  V+R+ VEKNRR +MK+L S+L SLLP+  S +  LTLP+Q+DEA  YIK+ +  
Sbjct: 8   TGQSRSVDRKTVEKNRRMQMKSLYSELISLLPHHSSTE-PLTLPDQLDEAANYIKKLQVN 67

Query: 71  VNAAKEKKERLMGKQSSTAATMEGSSSSSPAA--------PQLKIQEIGRSLQIFLSSGS 130
           V   +E+K  L+   +       GSSS S +         P+++IQE G    IFL +  
Sbjct: 68  VEKKRERKRNLVATTTLEKLNSVGSSSVSSSVDVSVPRKLPKIEIQETGSIFHIFLVTSL 127

Query: 131 DNQFLFRETIRILQQE-GAEVVSAGFSAAANSVLHTIHAQLGESMVEFGV-AKVSERLKS 183
           +++F+F E IR+L +E GAE+  AG+S   ++V HT+H ++ E   ++G  +++ ERL+ 
Sbjct: 128 EHKFMFCEIIRVLTEELGAEITHAGYSIVDDAVFHTLHCKVEEH--DYGARSQIPERLEK 186

[Close]

BLAST of MS028333 vs. ExPASy Swiss-Prot
Match: F4I4E1 (Transcription factor bHLH167 OS=Arabidopsis thaliana OX=3702 GN=BHLH167 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 5.4e-09
Identity = 55/185 (29.73%), Postives = 95/185 (51.35%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           M R  +    +SSS + +R + EK+RR  MK+L S L+S +    S    L +P  ID+A
Sbjct: 1   MGRAREIGEGNSSSLREQRNLREKDRRMRMKHLFSILSSHV----SPTRKLPVPHLIDQA 60

Query: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120
             Y+ + ++ VN  KEKK  L+  Q       EGS       P+L I+    ++++ L  
Sbjct: 61  TSYMIQLKENVNYLKEKKRTLL--QGELGNLYEGSF----LLPKLSIRSRDSTIEMNLIM 120

Query: 121 GSD-NQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLK 180
             +  + +  E + I ++EGA+V+SA      +   +TI AQ   S +    +++ ER++
Sbjct: 121 DLNMKRVMLHELVSIFEEEGAQVMSANLQNLNDRTTYTIIAQAIISRIGIDPSRIEERVR 175

Query: 181 SLVYG 185
            ++YG
Sbjct: 181 KIIYG 175

[Close]

BLAST of MS028333 vs. ExPASy Swiss-Prot
Match: Q9XIJ1 (Transcription factor bHLH168 OS=Arabidopsis thaliana OX=3702 GN=BHLH168 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 9.3e-09
Identity = 49/183 (26.78%), Postives = 96/183 (52.46%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           M+R  +    S+SS + +R + EK RR  MK+L S L+S +    S    L +P+ ID+A
Sbjct: 1   MERAREIGEGSASSLREQRNLREKERRMRMKHLFSILSSHV----SPTRRLPVPQLIDQA 60

Query: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120
           + Y+ + ++KVN   E K R++G +      ++  S  S   P+L I+ +   +++ L  
Sbjct: 61  VSYMIQLKEKVNYLNEMKRRMLGGE------VKNRSEGSSLLPKLSIRSLDSIIEMNLVM 120

Query: 121 GSDNQ-FLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLK 180
             + +  +  + + + ++EGA+V+SA      +   +TI AQ     +    +++ ERL+
Sbjct: 121 DLNMKGVMLHKLVSVFEEEGAQVMSANLQNLNDRTFYTIIAQAIICRIGIDPSRIEERLR 173

Query: 181 SLV 183
            ++
Sbjct: 181 DII 173

[Close]

BLAST of MS028333 vs. ExPASy Swiss-Prot
Match: Q0JFZ0 (Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. japonica OX=39947 GN=IRO2 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.9e-06
Identity = 50/184 (27.17%), Postives = 84/184 (45.65%), Query Frame = 0


Query: 8   SAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEAIKYIKEA 67
           S  S S  K+     E++RR ++  L S L +LLP  D++   L++P  +   +KYI E 
Sbjct: 62  SGGSGSHRKLSHNAYERDRRKQLNELYSSLRALLP--DADHTKLSIPTTVSRVLKYIPEL 121

Query: 68  EKKVNAAKEKKERLMGKQSSTAATMEG-------SSSSSPAAPQLKIQEIGRSLQIFLSS 127
           +K+V   + KK+ L    +ST     G       S   +P      I ++   +Q+ L S
Sbjct: 122 QKQVENLERKKKEL--TTTSTTNCKPGVLGSQLMSEGMAPIVSATCINDMEIMVQVSLLS 181

Query: 128 GSDNQFL-FRETIRILQQEGAEVVSAGFSAA-ANSVLHTIHAQLGESMVEFGVAKVSERL 183
                 L   + I++L+ EG   +S+  S+   N   ++IH Q  E  +        ERL
Sbjct: 182 NVAGSVLPLSKCIKVLENEGLHFISSSTSSGFGNRTFYSIHLQRSEGTINEECPAFCERL 241

[Close]

BLAST of MS028333 vs. ExPASy Swiss-Prot
Match: A2WZ60 (Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. indica OX=39946 GN=IRO2 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 9.6e-06
Identity = 50/184 (27.17%), Postives = 83/184 (45.11%), Query Frame = 0


Query: 8   SAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEAIKYIKEA 67
           S  S S  K+     E++RR ++  L S L +LLP  D  +  L++P  +   +KYI E 
Sbjct: 62  SGGSGSHRKLSHNAYERDRRKQLNELYSSLRALLPDADHTK-KLSIPTTVSRVLKYIPEL 121

Query: 68  EKKVNAAKEKKERLMGKQSSTAATMEG-------SSSSSPAAPQLKIQEIGRSLQIFLSS 127
           +K+V   + KK+ L    +ST     G       S   +P      I ++   +Q+ L S
Sbjct: 122 QKQVENLERKKKEL--TTTSTTNCKPGVLGSQLMSEGMAPIVSATCINDMEIMVQVSLLS 181

Query: 128 GSDNQFL-FRETIRILQQEGAEVVSAGFSAA-ANSVLHTIHAQLGESMVEFGVAKVSERL 183
                 L   + I++L+ EG   +S+  S+   N   ++IH Q  E  +        ERL
Sbjct: 182 NVAGSVLPLSKCIKVLENEGLHFISSSTSSGFGNRTFYSIHLQRSEGTINEECPAFCERL 241

[Close]

BLAST of MS028333 vs. ExPASy TrEMBL
Match: A0A6J1D2U8 (transcription factor bHLH168 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016789 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 3.7e-101
Identity = 204/211 (96.68%), Postives = 206/211 (97.63%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           MDRNFK SAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLP QDS+QISLTLPEQIDEA
Sbjct: 1   MDRNFKLSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPNQDSKQISLTLPEQIDEA 60

Query: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120
           IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS
Sbjct: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120

Query: 121 GSDNQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKS 180
           GSDNQFLFRETIRILQQEGAEVVSA FSAAANSVLHTIHAQLGESMVEFGVAKVSERLK 
Sbjct: 121 GSDNQFLFRETIRILQQEGAEVVSASFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKG 180

Query: 181 LVYGSGSDVELQ-EEQPWWDFEFPPDATWNF 211
           LVYGSGSDVELQ EEQPWW+FEFPPDATWNF
Sbjct: 181 LVYGSGSDVELQKEEQPWWEFEFPPDATWNF 211

[Close]

BLAST of MS028333 vs. ExPASy TrEMBL
Match: A0A6J1D2P8 (transcription factor bHLH162 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016789 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 2.6e-99
Identity = 203/211 (96.21%), Postives = 205/211 (97.16%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           MDRNFK SAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLP QDS +ISLTLPEQIDEA
Sbjct: 1   MDRNFKLSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPNQDS-KISLTLPEQIDEA 60

Query: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120
           IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS
Sbjct: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120

Query: 121 GSDNQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKS 180
           GSDNQFLFRETIRILQQEGAEVVSA FSAAANSVLHTIHAQLGESMVEFGVAKVSERLK 
Sbjct: 121 GSDNQFLFRETIRILQQEGAEVVSASFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKG 180

Query: 181 LVYGSGSDVELQ-EEQPWWDFEFPPDATWNF 211
           LVYGSGSDVELQ EEQPWW+FEFPPDATWNF
Sbjct: 181 LVYGSGSDVELQKEEQPWWEFEFPPDATWNF 210

[Close]

BLAST of MS028333 vs. ExPASy TrEMBL
Match: A0A6J1EW58 (transcription factor bHLH162-like OS=Cucurbita moschata OX=3662 GN=LOC111437113 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 4.2e-65
Identity = 142/206 (68.93%), Postives = 168/206 (81.55%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           MDR+F+FS +SSS++K+ERRIVEKNRRN+MK LCSQLNSLLP QDS++ISL LP+QIDEA
Sbjct: 50  MDRHFQFSTSSSSAAKIERRIVEKNRRNQMKTLCSQLNSLLPNQDSKEISLALPDQIDEA 109

Query: 61  IKYIKEAEKKVNAAKEKKERLMGK-QSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLS 120
           +KYIKE EKKVN AKEKKE+L GK +SS       SSSSS A+PQLKI + GRSL+I LS
Sbjct: 110 VKYIKELEKKVNLAKEKKEKLEGKNKSSMNIESSSSSSSSSASPQLKIHQSGRSLEIILS 169

Query: 121 SGSDNQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLK 180
           SG DNQ+LF ETI ILQ+EG EVV+A FS + +SV  T HAQL  SM+EF  AKV+ERLK
Sbjct: 170 SGLDNQYLFSETISILQEEGVEVVNASFSVSGSSVFQTFHAQLEGSMIEFEAAKVTERLK 229

Query: 181 SLVYGSGSDVELQEEQPWWDFEFPPD 206
            LVYGS SD+ELQ+E+ WWD EFP D
Sbjct: 230 RLVYGSNSDMELQKEKQWWD-EFPSD 254

[Close]

BLAST of MS028333 vs. ExPASy TrEMBL
Match: A0A5D3C788 (Transcription factor bHLH55-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003340 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 3.6e-64
Identity = 137/205 (66.83%), Postives = 168/205 (81.95%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           MD NF+FSA+SS+++K+ERRI+EKNRRN+MKNLC QL SL+P QDS+++SL LP+QID A
Sbjct: 1   MDPNFQFSASSSTAAKIERRIIEKNRRNQMKNLCDQLKSLVPQQDSKEVSLALPDQIDVA 60

Query: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120
           IKYIK+ EKKVN+AKEKK RL GK  S A  M+ SSSSS   PQLKI ++G+SL+I LSS
Sbjct: 61  IKYIKDLEKKVNSAKEKKNRLQGKNKS-AINMDSSSSSS---PQLKINQMGKSLEIVLSS 120

Query: 121 GSDNQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKS 180
           G DNQ+L  ET+RILQ+EG EVV+A FS +  SV HTIHA+LG+SMVEFG  K +ERLK 
Sbjct: 121 GIDNQYLLCETLRILQEEGIEVVNASFSVSGKSVFHTIHAELGDSMVEFGTTKATERLKR 180

Query: 181 LVYGSGSDVELQEEQPWWDFEFPPD 206
           LVYGS SDVELQ+E+ WWD EFP +
Sbjct: 181 LVYGSNSDVELQKEKQWWD-EFPSE 200

[Close]

BLAST of MS028333 vs. ExPASy TrEMBL
Match: A0A6J1JEF7 (transcription factor bHLH162-like OS=Cucurbita maxima OX=3661 GN=LOC111486157 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 3.0e-63
Identity = 139/205 (67.80%), Postives = 168/205 (81.95%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           MDR+F+FS +SSS++K+ERRI+EKNRRN+MK LCSQLNSLLP QDS++ISL+LP+QIDEA
Sbjct: 1   MDRHFQFSTSSSSAAKIERRIIEKNRRNQMKTLCSQLNSLLPNQDSKEISLSLPDQIDEA 60

Query: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120
           +KYIKE EKKVN AKEKKE+L GK  S    +E SSS S A+PQLKI + GRSL+I LSS
Sbjct: 61  VKYIKELEKKVNLAKEKKEKLEGKNKS-PMNIESSSSCS-ASPQLKIHQSGRSLEIILSS 120

Query: 121 GSDNQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLKS 180
           G DNQ+LF ETI ILQ+EG EVV+A FS + +SV  T HAQL  S++EF  AKV+ERLK 
Sbjct: 121 GLDNQYLFSETISILQKEGVEVVNASFSVSGSSVFQTFHAQLEGSIIEFEAAKVTERLKR 180

Query: 181 LVYGSGSDVELQEEQPWWDFEFPPD 206
           LVYGS SD+ELQ+E+ WWD EFP D
Sbjct: 181 LVYGSNSDMELQKEKQWWD-EFPSD 202

[Close]

BLAST of MS028333 vs. TAIR 10
Match: AT4G20970.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 8.9e-23
Identity = 70/182 (38.46%), Postives = 111/182 (60.99%), Query Frame = 0


Query: 11  SSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEAIKYIKEAEKK 70
           +  S  V+R+ VEKNRR +MK+L S+L SLLP+  S +  LTLP+Q+DEA  YIK+ +  
Sbjct: 8   TGQSRSVDRKTVEKNRRMQMKSLYSELISLLPHHSSTE-PLTLPDQLDEAANYIKKLQVN 67

Query: 71  VNAAKEKKERLMGKQSSTAATMEGSSSSSPAA--------PQLKIQEIGRSLQIFLSSGS 130
           V   +E+K  L+   +       GSSS S +         P+++IQE G    IFL +  
Sbjct: 68  VEKKRERKRNLVATTTLEKLNSVGSSSVSSSVDVSVPRKLPKIEIQETGSIFHIFLVTSL 127

Query: 131 DNQFLFRETIRILQQE-GAEVVSAGFSAAANSVLHTIHAQLGESMVEFGV-AKVSERLKS 183
           +++F+F E IR+L +E GAE+  AG+S   ++V HT+H ++ E   ++G  +++ ERL+ 
Sbjct: 128 EHKFMFCEIIRVLTEELGAEITHAGYSIVDDAVFHTLHCKVEEH--DYGARSQIPERLEK 186

[Close]

BLAST of MS028333 vs. TAIR 10
Match: AT1G10585.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 62.8 bits (151), Expect = 3.9e-10
Identity = 55/185 (29.73%), Postives = 95/185 (51.35%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           M R  +    +SSS + +R + EK+RR  MK+L S L+S +    S    L +P  ID+A
Sbjct: 1   MGRAREIGEGNSSSLREQRNLREKDRRMRMKHLFSILSSHV----SPTRKLPVPHLIDQA 60

Query: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120
             Y+ + ++ VN  KEKK  L+  Q       EGS       P+L I+    ++++ L  
Sbjct: 61  TSYMIQLKENVNYLKEKKRTLL--QGELGNLYEGSF----LLPKLSIRSRDSTIEMNLIM 120

Query: 121 GSD-NQFLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLK 180
             +  + +  E + I ++EGA+V+SA      +   +TI AQ   S +    +++ ER++
Sbjct: 121 DLNMKRVMLHELVSIFEEEGAQVMSANLQNLNDRTTYTIIAQAIISRIGIDPSRIEERVR 175

Query: 181 SLVYG 185
            ++YG
Sbjct: 181 KIIYG 175

[Close]

BLAST of MS028333 vs. TAIR 10
Match: AT1G10586.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 6.6e-10
Identity = 49/183 (26.78%), Postives = 96/183 (52.46%), Query Frame = 0


Query: 1   MDRNFKFSAASSSSSKVERRIVEKNRRNEMKNLCSQLNSLLPYQDSEQISLTLPEQIDEA 60
           M+R  +    S+SS + +R + EK RR  MK+L S L+S +    S    L +P+ ID+A
Sbjct: 1   MERAREIGEGSASSLREQRNLREKERRMRMKHLFSILSSHV----SPTRRLPVPQLIDQA 60

Query: 61  IKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEIGRSLQIFLSS 120
           + Y+ + ++KVN   E K R++G +      ++  S  S   P+L I+ +   +++ L  
Sbjct: 61  VSYMIQLKEKVNYLNEMKRRMLGGE------VKNRSEGSSLLPKLSIRSLDSIIEMNLVM 120

Query: 121 GSDNQ-FLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEFGVAKVSERLK 180
             + +  +  + + + ++EGA+V+SA      +   +TI AQ     +    +++ ERL+
Sbjct: 121 DLNMKGVMLHKLVSVFEEEGAQVMSANLQNLNDRTFYTIIAQAIICRIGIDPSRIEERLR 173

Query: 181 SLV 183
            ++
Sbjct: 181 DII 173

[Close]

BLAST of MS028333 vs. TAIR 10
Match: AT1G10586.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 7.5e-06
Identity = 32/133 (24.06%), Postives = 70/133 (52.63%), Query Frame = 0


Query: 51  LTLPEQIDEAIKYIKEAEKKVNAAKEKKERLMGKQSSTAATMEGSSSSSPAAPQLKIQEI 110
           L +P+ ID+A+ Y+ + ++KVN   E K R++G +      ++  S  S   P+L I+ +
Sbjct: 6   LPVPQLIDQAVSYMIQLKEKVNYLNEMKRRMLGGE------VKNRSEGSSLLPKLSIRSL 65

Query: 111 GRSLQIFLSSGSDNQ-FLFRETIRILQQEGAEVVSAGFSAAANSVLHTIHAQLGESMVEF 170
              +++ L    + +  +  + + + ++EGA+V+SA      +   +TI AQ     +  
Sbjct: 66  DSIIEMNLVMDLNMKGVMLHKLVSVFEEEGAQVMSANLQNLNDRTFYTIIAQAIICRIGI 125

Query: 171 GVAKVSERLKSLV 183
             +++ ERL+ ++
Sbjct: 126 DPSRIEERLRDII 132

The following BLAST results are available for this feature:

BLAST of MS028333 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147998.1	7.6e-101	96.68	transcription factor bHLH168 isoform X1 [Momordica charantia]	[more]
XP_022147999.1	5.4e-99	96.21	transcription factor bHLH162 isoform X2 [Momordica charantia]	[more]
XP_038888628.1	1.9e-67	69.46	transcription factor bHLH162-like [Benincasa hispida]	[more]
XP_022930718.1	8.8e-65	68.93	transcription factor bHLH162-like [Cucurbita moschata]	[more]
XP_023531689.1	2.6e-64	69.27	transcription factor bHLH162-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS028333 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JIJ7	1.2e-21	38.46	Transcription factor bHLH162 OS=Arabidopsis thaliana OX=3702 GN=BHLH162 PE=1 SV=...	[more]
F4I4E1	5.4e-09	29.73	Transcription factor bHLH167 OS=Arabidopsis thaliana OX=3702 GN=BHLH167 PE=2 SV=...	[more]
Q9XIJ1	9.3e-09	26.78	Transcription factor bHLH168 OS=Arabidopsis thaliana OX=3702 GN=BHLH168 PE=3 SV=...	[more]
Q0JFZ0	1.9e-06	27.17	Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. japonica OX=3...	[more]
A2WZ60	9.6e-06	27.17	Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. indica OX=399...	[more]

BLAST of MS028333 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2U8	3.7e-101	96.68	transcription factor bHLH168 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1D2P8	2.6e-99	96.21	transcription factor bHLH162 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1EW58	4.2e-65	68.93	transcription factor bHLH162-like OS=Cucurbita moschata OX=3662 GN=LOC111437113 ...	[more]
A0A5D3C788	3.6e-64	66.83	Transcription factor bHLH55-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A6J1JEF7	3.0e-63	67.80	transcription factor bHLH162-like OS=Cucurbita maxima OX=3661 GN=LOC111486157 PE...	[more]

BLAST of MS028333 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20970.1	8.9e-23	38.46	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G10585.1	3.9e-10	29.73	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G10586.1	6.6e-10	26.78	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G10586.2	7.5e-06	24.06	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 57..84
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 75..102
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..16
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 84..102
None	No IPR available	PANTHER	PTHR13935:SF63	HELIX LOOP HELIX DNA-BINDING DOMAIN PROTEIN	coord: 11..189
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	4.10.280.10		coord: 8..96 e-value: 2.4E-8 score: 35.7
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 13..87
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 16..66 e-value: 2.7E-5 score: 24.1
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 14..67 score: 10.632602
IPR015660	Achaete-scute transcription factor-related	PANTHER	PTHR13935	ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED	coord: 11..189

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS028333.1	MS028333.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0046983	protein dimerization activity