MS028232 (gene) Bitter gourd (TR) v1

BLAST of MS028232 vs. NCBI nr
Match: XP_022136073.1 (BURP domain-containing protein BNM2A-like [Momordica charantia])

HSP 1 Score: 591.3 bits (1523), Expect = 4.8e-165
Identity = 298/302 (98.68%), Postives = 298/302 (98.68%), Query Frame = 0

Query: 1   MGGRVASCLILLLLFL--FDEASAEVGEKSKQMEAESADNGVIKMVTKMAYNSQELNSMA 60
           MGGRVASCLILLLLFL  FDEASAEVGEKSKQMEAESADNGVIKMVTKMAYNSQELNSMA
Sbjct: 1   MGGRVASCLILLLLFLVMFDEASAEVGEKSKQMEAESADNGVIKMVTKMAYNSQELNSMA 60

Query: 61  DQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIADSI 120
           DQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIADSI
Sbjct: 61  DQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIADSI 120

Query: 121 PFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSMESMADFARLAL 180
           PFSLEQLPEILRFFSFSPGSRQAR VETTLHQCEHKRVEGETKFCSTSMESMADFARLAL
Sbjct: 121 PFSLEQLPEILRFFSFSPGSRQARAVETTLHQCEHKRVEGETKFCSTSMESMADFARLAL 180

Query: 181 GLASKSSLKHLVTTHLTKSRPPYLQNYTLLQMPNAVTKFLACHLMTYPYAVHYCHYLHGG 240
           GLASKSSLKHLVTTHLTK RPPYLQNYTLLQMPNAVTKFLACHLMTYPYAVHYCHYLHGG
Sbjct: 181 GLASKSSLKHLVTTHLTKPRPPYLQNYTLLQMPNAVTKFLACHLMTYPYAVHYCHYLHGG 240

Query: 241 DKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPNDLILI 300
           DKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPNDLILI
Sbjct: 241 DKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPNDLILI 300

BLAST of MS028232 vs. NCBI nr
Match: XP_022135717.1 (BURP domain-containing protein BNM2C-like [Momordica charantia])

HSP 1 Score: 402.5 bits (1033), Expect = 3.2e-108
Identity = 213/306 (69.61%), Postives = 245/306 (80.07%), Query Frame = 0

Query: 1   MGGRVASCLIL--LLLFLFDEASAEVGEKSKQMEAESADNGVIKMVTKMAYNSQELNSMA 60
           MGGRV S LIL  LLL +F EASAEVGEKSKQMEA S+D+ V++MVTKM  NSQELNS  
Sbjct: 1   MGGRVTSYLILLHLLLVMFAEASAEVGEKSKQMEAASSDSSVLRMVTKMTANSQELNS-- 60

Query: 61  DQQHHADDSSSSHMEHMNDPA-QIFFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIADS 120
                        ME+MNDP+  IFFT +DLKLGKKLPL L PKDPST+PPM  KE+ADS
Sbjct: 61  -------------MEYMNDPSNMIFFTTDDLKLGKKLPLNLPPKDPSTTPPMWPKEMADS 120

Query: 121 IPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSMESMADFARLA 180
           IPFSLEQLP+IL FFSFS GS QA+ VE+ LH+C+ +  +GETKFC TSMESMAD AR A
Sbjct: 121 IPFSLEQLPQILNFFSFSLGSPQAQAVESALHRCDIEPNKGETKFCGTSMESMADSARSA 180

Query: 181 LGLASKSSLKHLVTT-HLTKSRPPYLQNYTLLQMPN--AVTKFLACHLMTYPYAVHYCHY 240
            GL SKSS+K+L T+ H T+SRPPYLQNYTLLQMPN  A  KFLACH + YPYAVHYCHY
Sbjct: 181 FGLGSKSSVKYLATSAHSTRSRPPYLQNYTLLQMPNEIAAPKFLACHAIPYPYAVHYCHY 240

Query: 241 LHGGDKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPND 300
           L G +K LQI LEG+NGNVVMAAAVCH+DTS W+P+HVSF+VLK+KPG+ P+CH+FPPND
Sbjct: 241 LEGDNKVLQIPLEGENGNVVMAAAVCHVDTSEWSPEHVSFKVLKSKPGMTPVCHVFPPND 291

BLAST of MS028232 vs. NCBI nr
Match: XP_022135666.1 (BURP domain protein USPL1-like [Momordica charantia])

HSP 1 Score: 298.9 bits (764), Expect = 4.9e-77
Identity = 168/306 (54.90%), Postives = 203/306 (66.34%), Query Frame = 0

Query: 1   MGGRVASCLIL---LLLFLFDEASAEVGEKSKQME--AESADNGVIKMVTKMAYNSQELN 60
           MGGRVAS LI+   LLL +F EA A VGEKSKQME   +  +  VIKMVT+M        
Sbjct: 1   MGGRVASSLIILLHLLLLMFAEALAAVGEKSKQMENLNDHIEASVIKMVTQM-------- 60

Query: 61  SMADQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIA 120
                    D+  SSHMEH N    +FFTINDLK G+KLP+Y   +DPS SPP + KE A
Sbjct: 61  --------EDNFHSSHMEHTNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKA 120

Query: 121 DSIPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSMESMADFAR 180
           D IPFSL++LP+IL FFSF   S Q++ V+ TL QCE K ++GETKFC+TSMESM DF  
Sbjct: 121 DPIPFSLKKLPQILSFFSFPLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVG 180

Query: 181 LALGLASKSSLKHLVTTHLTKSRPPYLQNYTLLQMPN--AVTKFLACHLMTYPYAVHYCH 240
            ALG    SS + L T+HLTKS   + QNYT+   P   A  K +ACH M YPYAV+YCH
Sbjct: 181 TALG-TKPSSFQLLTTSHLTKSL-VHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYYCH 240

Query: 241 YLHGGDKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPN 300
           Y  G +  L+I LEGDN + V A AVCHMDTS W+P H +FRVLKT+PG  P+CH FP +
Sbjct: 241 YQEGDNSVLKIPLEGDNSDRVQAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPAD 288

BLAST of MS028232 vs. NCBI nr
Match: XP_022971234.1 (BURP domain protein USPL1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 288.9 bits (738), Expect = 5.1e-74
Identity = 158/313 (50.48%), Postives = 207/313 (66.13%), Query Frame = 0

Query: 2   GGRVASCLI---LLLLFLFDEAS---AEVGEKSKQ------MEAESADNGVIKMVTKMAY 61
           G  + SC +   LLLL +F EA    +E+ EK+KQ      +EA      +IKMVTKMA 
Sbjct: 3   GSTMTSCFLLLPLLLLVMFGEARQAFSEIEEKNKQIELNDHIEATFKPKPIIKMVTKMA- 62

Query: 62  NSQELNSMADQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSPPM 121
                    D   H   SSSSHM+H+N   ++FFTINDLK GKKLP+Y   +DPS SPP+
Sbjct: 63  --------NDHNSHDTHSSSSHMDHINPLLRVFFTINDLKQGKKLPIYFPKRDPSKSPPL 122

Query: 122 VAKEIADSIPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSMES 181
           + KE AD IPFSL++LP+IL +FS+   S QA+ V+ TL QCE K ++GETKFC+TSMES
Sbjct: 123 LPKEQADPIPFSLKKLPQILSYFSYPSNSPQAQAVKETLEQCELKPIKGETKFCATSMES 182

Query: 182 MADFARLALGLASKSSLKHLVTTHLTKSRPPYLQNYTLLQMPN--AVTKFLACHLMTYPY 241
           M +F R A G   K+  + L T+HLTKS   +LQNYT ++ P   A  + +ACH M Y Y
Sbjct: 183 MVNFVRTAFG--PKTHYEVLTTSHLTKSN-VHLQNYTFIEAPKEIAAPRLVACHTMPYAY 242

Query: 242 AVHYCHYLHGGDKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPIC 301
           AV+YCHY  G +  L+I LEG+NG+ V A A+CHMDTS W+P H SF+VLK +PG +P+C
Sbjct: 243 AVYYCHYQEGDNNVLKIQLEGENGDRVEALAICHMDTSQWSPSHPSFQVLKIQPGTIPVC 302

BLAST of MS028232 vs. NCBI nr
Match: XP_022927168.1 (BURP domain protein USPL1-like isoform X1 [Cucurbita moschata] >KAG6588446.1 BURP domain protein USPL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 286.6 bits (732), Expect = 2.5e-73
Identity = 160/315 (50.79%), Postives = 208/315 (66.03%), Query Frame = 0

Query: 1   MGG-RVASCLI---LLLLFLFDEAS---AEVGEKSKQ------MEAESADNGVIKMVTKM 60
           MGG  + SC +   LLLL +F EA    +E+ EK+KQ      +EA      +IKMVTKM
Sbjct: 1   MGGTTMTSCFLLLPLLLLVMFGEARQTFSEIEEKNKQIELNDHIEATFKPKPIIKMVTKM 60

Query: 61  AYNSQELNSMADQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSP 120
           A          D   H   SSSSHM+H+N    +FFTINDLK GKKLP+Y   +DPS SP
Sbjct: 61  A---------NDHNSHDTHSSSSHMDHINPLLIVFFTINDLKQGKKLPIYFPKRDPSKSP 120

Query: 121 PMVAKEIADSIPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSM 180
           P++ KE AD IPFSL++LP+IL +FS+   S QA+ V+ TL QCE K ++GETKFC+TSM
Sbjct: 121 PLLPKEQADPIPFSLKKLPQILSYFSYPSNSPQAQAVKETLEQCELKPIKGETKFCATSM 180

Query: 181 ESMADFARLALGLASKSSLKHLVTTHLTKSRPPYLQNYTLLQMPN--AVTKFLACHLMTY 240
           ESM +F R A G   K+  + L T+HLTKS   +LQNYT ++ P   A  + +ACH M Y
Sbjct: 181 ESMVNFVRTAFG--PKTHYEVLTTSHLTKSN-VHLQNYTFIEAPKEIAAPRLVACHTMPY 240

Query: 241 PYAVHYCHYLHGGDKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVP 300
            YAV+YCHY  G +  L+I LEG+NG+ V A A+CHMDTS W+P H SF+VLK +PG +P
Sbjct: 241 AYAVYYCHYQEGDNNVLKIQLEGENGDRVEALAICHMDTSQWSPSHPSFQVLKIQPGTIP 300

BLAST of MS028232 vs. ExPASy Swiss-Prot
Match: O65009 (BURP domain-containing protein BNM2A OS=Brassica napus OX=3708 GN=BNM2A PE=2 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 2.0e-44
Identity = 104/257 (40.47%), Postives = 149/257 (57.98%), Query Frame = 0

Query: 48  AYNSQELNSMADQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSP 107
           AY S++L S  +Q+           E  +    +FF I+DLKLG KLP+Y    D    P
Sbjct: 26  AYTSRKLISNNEQEGQNISHLFKDGEFEDPTMYMFFKISDLKLGTKLPIYFNKNDLRKVP 85

Query: 108 PMVAKEIADSIPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSM 167
           P++ ++ AD IPFS   L  +L  FS S  S Q + ++ TL +C+ K +EGE KFC TS+
Sbjct: 86  PLLTRQEADLIPFSESNLDFLLNHFSISKDSPQGKAMKETLKRCDFKAIEGEYKFCGTSL 145

Query: 168 ESMADFARLALGLASKSSLKHLVTTHLT--KSRPPY-LQNYTLLQMPNAV--TKFLACHL 227
           ESM D A+    +AS + LK + T  +   ++R  Y L NYT  ++P  +   K L CH 
Sbjct: 146 ESMLDLAKKT--IASNADLKVMTTKVMVPDQNRISYALHNYTFAEVPKELDGIKVLGCHR 205

Query: 228 MTYPYAVHYCHYLHGGDKALQILLEGDNG-NVVMAAAVCHMDTSGWTPDHVSFRVLKTKP 287
           M YPY V+YCH    G K  ++ L  D+G  +V+  AVCHMDTS W  DHV+F+VLK +P
Sbjct: 206 MPYPYVVYYCHGHKSGTKVFEVNLMSDDGIQLVVGPAVCHMDTSMWNADHVAFKVLKIEP 265

Query: 288 GIVPICHIFPPNDLILI 299
              P+CH FP ++++ +
Sbjct: 266 RSAPVCHFFPLDNIVWV 280

BLAST of MS028232 vs. ExPASy Swiss-Prot
Match: D2CZZ9 (BURP domain-containing protein BNM2C OS=Brassica napus OX=3708 GN=BNM2C PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 2.8e-43
Identity = 103/257 (40.08%), Postives = 147/257 (57.20%), Query Frame = 0

Query: 48  AYNSQELNSMADQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSP 107
           A  S++L S  +Q+           E  +    +FF I+DLKLG KLP+Y    D    P
Sbjct: 25  ACTSRKLISNNEQEGQNISHLFKDGEFEDPTMYMFFKISDLKLGTKLPIYFNKNDLRKVP 84

Query: 108 PMVAKEIADSIPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSM 167
           P++ ++ AD IPFS   L  +L  FS S  S Q   ++ TL +C+ K +EGE KFC TS+
Sbjct: 85  PLLTRQEADLIPFSESNLDFLLNHFSISKDSPQGEAMKETLQRCDFKAIEGEYKFCGTSL 144

Query: 168 ESMADFARLALGLASKSSLKHLVTTHLT--KSRPPY-LQNYTLLQMPNAV--TKFLACHL 227
           ESM D A+    +AS + LK + T  +   ++R  Y L NYT  ++P  +   K L CH 
Sbjct: 145 ESMLDLAKKT--IASNADLKVMTTKVMVPDQNRISYALHNYTFAEVPKELDGIKVLGCHR 204

Query: 228 MTYPYAVHYCHYLHGGDKALQILLEGDNG-NVVMAAAVCHMDTSGWTPDHVSFRVLKTKP 287
           M YPY V+YCH    G K  ++ L  D+G  +V+  AVCHMDTS W  DHV+F+VLK +P
Sbjct: 205 MPYPYVVYYCHGHKSGTKVFEVNLMSDDGIQLVVGPAVCHMDTSMWNADHVAFKVLKIEP 264

Query: 288 GIVPICHIFPPNDLILI 299
              P+CH FP ++++ +
Sbjct: 265 RSAPVCHFFPLDNIVWV 279

BLAST of MS028232 vs. ExPASy Swiss-Prot
Match: Q9XI99 (BURP domain protein USPL1 OS=Arabidopsis thaliana OX=3702 GN=USPL1 PE=2 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 9.1e-42
Identity = 98/258 (37.98%), Postives = 150/258 (58.14%), Query Frame = 0

Query: 48  AYNSQELNSMADQQHHADDSSSSHMEHMNDPA-QIFFTINDLKLGKKLPLYLAPKDPSTS 107
           A+ S++L S+ +++   D S        +DP+  ++FT+NDLKLG KL +Y    D    
Sbjct: 24  AHTSRKLISIKEKEGQ-DISHLLKDGEFDDPSLYMYFTLNDLKLGTKLLIYFYKNDLQKL 83

Query: 108 PPMVAKEIADSIPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTS 167
           PP++ ++ AD IPF+  +L  +L  FS +  S Q + ++ TL  C+ K +EGE KFC TS
Sbjct: 84  PPLLTRQQADLIPFTKSKLDFLLDHFSITKDSPQGKAIKETLGHCDAKAIEGEHKFCGTS 143

Query: 168 MESMADFARLALGLASKSSLKHLVTTHLTKSRPPY---LQNYTLLQMPNAVT--KFLACH 227
           +ES+ D  +  +G      LK + T  +  ++      L NYT ++ P  +   K L CH
Sbjct: 144 LESLIDLVKKTMGY--NVDLKVMTTKVMVPAQNSISYALHNYTFVEAPKELVGIKMLGCH 203

Query: 228 LMTYPYAVHYCHYLHGGDKALQILLEGDNGNV-VMAAAVCHMDTSGWTPDHVSFRVLKTK 287
            M YPYAV+YCH   GG +  ++ L  D+G   V+  AVCHMDTS W  DHV+F+VLK +
Sbjct: 204 RMPYPYAVYYCHGHKGGSRVFEVNLVTDDGRQRVVGPAVCHMDTSTWDADHVAFKVLKME 263

Query: 288 PGIVPICHIFPPNDLILI 299
           P   P+CH FP ++++ +
Sbjct: 264 PRSAPVCHFFPLDNIVWV 278

BLAST of MS028232 vs. ExPASy Swiss-Prot
Match: B9G9L9 (BURP domain-containing protein 17 OS=Oryza sativa subsp. japonica OX=39947 GN=BURP17 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.7e-35
Identity = 91/249 (36.55%), Postives = 132/249 (53.01%), Query Frame = 0

Query: 53  ELNSMADQQHHADDSSSSHMEHMNDP-AQIFFTINDLKLGKKL--PLYLAPKDPSTSPPM 112
           EL    D Q +   S     E + DP   +FF   +L+ GKK+   L+ A    +T+   
Sbjct: 333 ELVHYTDGQRNYKSSVLETPELLKDPDMALFFLEKNLQQGKKINNALHFANLLATTNSKF 392

Query: 113 VAKEIADSIPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSMES 172
           + +  ADSIPFS ++LPEIL  F   PGS  A ++  TL  CE    +GE K C+TS+ES
Sbjct: 393 LPRGKADSIPFSSKELPEILDRFGVRPGSDDAAEMSATLQDCELPANKGEKKACATSLES 452

Query: 173 MADFARLALGLASKSSLKHLVTTHLTKSRPPYLQNYTL--LQMPNAVTKFLACHLMTYPY 232
           + DF   + G +   +   +V +   +S     Q+YT+  ++      + +ACH  +YPY
Sbjct: 453 IVDFVTSSFGASDVDAASTVVLSKAVES-SSLAQDYTVSGVRRMAGTGQLIACHPESYPY 512

Query: 233 AVHYCHYLHGGDKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPIC 292
           AV  CH      +A +  L G +G  V A AVCH DTS W P+H +F VL  KPG VP+C
Sbjct: 513 AVFMCHLTEATTRAYKASLVGKDGTAVEAVAVCHTDTSDWNPEHAAFHVLGVKPGTVPVC 572

Query: 293 HIFPPNDLI 297
           H   P+ ++
Sbjct: 573 HFMQPDAVV 580

BLAST of MS028232 vs. ExPASy Swiss-Prot
Match: Q942D4 (BURP domain-containing protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=BURP3 PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 4.8e-35
Identity = 87/228 (38.16%), Postives = 124/228 (54.39%), Query Frame = 0

Query: 74  HMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIADSIPFSLEQLPEILRFFS 133
           H +    +FF   DL  GK + ++      +     + +  AD++PFS E++PEIL  FS
Sbjct: 205 HDDPNVALFFLEKDLHPGKTMAVHFTA--TTAGEKFLPRSEADAMPFSSEKVPEILSRFS 264

Query: 134 FSPGSRQARDVETTLHQCEHKRVEGETKFCSTSMESMADFARLALGLASKSSLKHLVTTH 193
             PGS +A ++  TL  CE    +GE K C+TS+ESM DFA  +LG    +S     +T 
Sbjct: 265 VKPGSVEAAEMAQTLRDCEAPPAQGERKACATSLESMVDFATSSLG----TSHVRAASTV 324

Query: 194 LTKSRPPYLQNYTLLQMPNAVT-----KFLACHLMTYPYAVHYCHYLHGGDKALQILLEG 253
           + K   P  Q YT+  +  A       + +ACH   Y YAV  CH L    +A  + + G
Sbjct: 325 VGKEGSPE-QEYTVTAVKRAAAGGDQDQLVACHAEPYAYAVFACH-LTRATRAYAVSMAG 384

Query: 254 DNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPNDLI 297
            +G  V A AVCH DT+GW P HV+F+VLK KPG VP+CH  P + ++
Sbjct: 385 RDGTGVEAVAVCHADTAGWNPKHVAFQVLKVKPGTVPVCHFLPQDHVV 424

BLAST of MS028232 vs. ExPASy TrEMBL
Match: A0A6J1C6L7 (BURP domain-containing protein BNM2A-like OS=Momordica charantia OX=3673 GN=LOC111007856 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.3e-165
Identity = 298/302 (98.68%), Postives = 298/302 (98.68%), Query Frame = 0

Query: 1   MGGRVASCLILLLLFL--FDEASAEVGEKSKQMEAESADNGVIKMVTKMAYNSQELNSMA 60
           MGGRVASCLILLLLFL  FDEASAEVGEKSKQMEAESADNGVIKMVTKMAYNSQELNSMA
Sbjct: 1   MGGRVASCLILLLLFLVMFDEASAEVGEKSKQMEAESADNGVIKMVTKMAYNSQELNSMA 60

Query: 61  DQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIADSI 120
           DQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIADSI
Sbjct: 61  DQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIADSI 120

Query: 121 PFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSMESMADFARLAL 180
           PFSLEQLPEILRFFSFSPGSRQAR VETTLHQCEHKRVEGETKFCSTSMESMADFARLAL
Sbjct: 121 PFSLEQLPEILRFFSFSPGSRQARAVETTLHQCEHKRVEGETKFCSTSMESMADFARLAL 180

Query: 181 GLASKSSLKHLVTTHLTKSRPPYLQNYTLLQMPNAVTKFLACHLMTYPYAVHYCHYLHGG 240
           GLASKSSLKHLVTTHLTK RPPYLQNYTLLQMPNAVTKFLACHLMTYPYAVHYCHYLHGG
Sbjct: 181 GLASKSSLKHLVTTHLTKPRPPYLQNYTLLQMPNAVTKFLACHLMTYPYAVHYCHYLHGG 240

Query: 241 DKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPNDLILI 300
           DKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPNDLILI
Sbjct: 241 DKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPNDLILI 300

BLAST of MS028232 vs. ExPASy TrEMBL
Match: A0A6J1C3I5 (BURP domain-containing protein BNM2C-like OS=Momordica charantia OX=3673 GN=LOC111007610 PE=4 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 1.5e-108
Identity = 213/306 (69.61%), Postives = 245/306 (80.07%), Query Frame = 0

Query: 1   MGGRVASCLIL--LLLFLFDEASAEVGEKSKQMEAESADNGVIKMVTKMAYNSQELNSMA 60
           MGGRV S LIL  LLL +F EASAEVGEKSKQMEA S+D+ V++MVTKM  NSQELNS  
Sbjct: 1   MGGRVTSYLILLHLLLVMFAEASAEVGEKSKQMEAASSDSSVLRMVTKMTANSQELNS-- 60

Query: 61  DQQHHADDSSSSHMEHMNDPA-QIFFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIADS 120
                        ME+MNDP+  IFFT +DLKLGKKLPL L PKDPST+PPM  KE+ADS
Sbjct: 61  -------------MEYMNDPSNMIFFTTDDLKLGKKLPLNLPPKDPSTTPPMWPKEMADS 120

Query: 121 IPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSMESMADFARLA 180
           IPFSLEQLP+IL FFSFS GS QA+ VE+ LH+C+ +  +GETKFC TSMESMAD AR A
Sbjct: 121 IPFSLEQLPQILNFFSFSLGSPQAQAVESALHRCDIEPNKGETKFCGTSMESMADSARSA 180

Query: 181 LGLASKSSLKHLVTT-HLTKSRPPYLQNYTLLQMPN--AVTKFLACHLMTYPYAVHYCHY 240
            GL SKSS+K+L T+ H T+SRPPYLQNYTLLQMPN  A  KFLACH + YPYAVHYCHY
Sbjct: 181 FGLGSKSSVKYLATSAHSTRSRPPYLQNYTLLQMPNEIAAPKFLACHAIPYPYAVHYCHY 240

Query: 241 LHGGDKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPND 300
           L G +K LQI LEG+NGNVVMAAAVCH+DTS W+P+HVSF+VLK+KPG+ P+CH+FPPND
Sbjct: 241 LEGDNKVLQIPLEGENGNVVMAAAVCHVDTSEWSPEHVSFKVLKSKPGMTPVCHVFPPND 291

BLAST of MS028232 vs. ExPASy TrEMBL
Match: A0A6J1C1E3 (BURP domain protein USPL1-like OS=Momordica charantia OX=3673 GN=LOC111007566 PE=4 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 2.4e-77
Identity = 168/306 (54.90%), Postives = 203/306 (66.34%), Query Frame = 0

Query: 1   MGGRVASCLIL---LLLFLFDEASAEVGEKSKQME--AESADNGVIKMVTKMAYNSQELN 60
           MGGRVAS LI+   LLL +F EA A VGEKSKQME   +  +  VIKMVT+M        
Sbjct: 1   MGGRVASSLIILLHLLLLMFAEALAAVGEKSKQMENLNDHIEASVIKMVTQM-------- 60

Query: 61  SMADQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIA 120
                    D+  SSHMEH N    +FFTINDLK G+KLP+Y   +DPS SPP + KE A
Sbjct: 61  --------EDNFHSSHMEHTNPQLIVFFTINDLKQGQKLPIYFPKRDPSKSPPFLPKEKA 120

Query: 121 DSIPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSMESMADFAR 180
           D IPFSL++LP+IL FFSF   S Q++ V+ TL QCE K ++GETKFC+TSMESM DF  
Sbjct: 121 DPIPFSLKKLPQILSFFSFPLNSPQSQAVKETLQQCELKPIKGETKFCATSMESMVDFVG 180

Query: 181 LALGLASKSSLKHLVTTHLTKSRPPYLQNYTLLQMPN--AVTKFLACHLMTYPYAVHYCH 240
            ALG    SS + L T+HLTKS   + QNYT+   P   A  K +ACH M YPYAV+YCH
Sbjct: 181 TALG-TKPSSFQLLTTSHLTKSL-VHFQNYTISGAPEVIAAPKLVACHAMPYPYAVYYCH 240

Query: 241 YLHGGDKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPN 300
           Y  G +  L+I LEGDN + V A AVCHMDTS W+P H +FRVLKT+PG  P+CH FP +
Sbjct: 241 YQEGDNSVLKIPLEGDNSDRVQAMAVCHMDTSQWSPSHPAFRVLKTEPGANPVCHFFPAD 288

BLAST of MS028232 vs. ExPASy TrEMBL
Match: A0A6J1I7Z5 (BURP domain protein USPL1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470018 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 2.5e-74
Identity = 158/313 (50.48%), Postives = 207/313 (66.13%), Query Frame = 0

Query: 2   GGRVASCLI---LLLLFLFDEAS---AEVGEKSKQ------MEAESADNGVIKMVTKMAY 61
           G  + SC +   LLLL +F EA    +E+ EK+KQ      +EA      +IKMVTKMA 
Sbjct: 3   GSTMTSCFLLLPLLLLVMFGEARQAFSEIEEKNKQIELNDHIEATFKPKPIIKMVTKMA- 62

Query: 62  NSQELNSMADQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSPPM 121
                    D   H   SSSSHM+H+N   ++FFTINDLK GKKLP+Y   +DPS SPP+
Sbjct: 63  --------NDHNSHDTHSSSSHMDHINPLLRVFFTINDLKQGKKLPIYFPKRDPSKSPPL 122

Query: 122 VAKEIADSIPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSMES 181
           + KE AD IPFSL++LP+IL +FS+   S QA+ V+ TL QCE K ++GETKFC+TSMES
Sbjct: 123 LPKEQADPIPFSLKKLPQILSYFSYPSNSPQAQAVKETLEQCELKPIKGETKFCATSMES 182

Query: 182 MADFARLALGLASKSSLKHLVTTHLTKSRPPYLQNYTLLQMPN--AVTKFLACHLMTYPY 241
           M +F R A G   K+  + L T+HLTKS   +LQNYT ++ P   A  + +ACH M Y Y
Sbjct: 183 MVNFVRTAFG--PKTHYEVLTTSHLTKSN-VHLQNYTFIEAPKEIAAPRLVACHTMPYAY 242

Query: 242 AVHYCHYLHGGDKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPIC 301
           AV+YCHY  G +  L+I LEG+NG+ V A A+CHMDTS W+P H SF+VLK +PG +P+C
Sbjct: 243 AVYYCHYQEGDNNVLKIQLEGENGDRVEALAICHMDTSQWSPSHPSFQVLKIQPGTIPVC 302

BLAST of MS028232 vs. ExPASy TrEMBL
Match: A0A6J1EK95 (BURP domain protein USPL1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434097 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.2e-73
Identity = 160/315 (50.79%), Postives = 208/315 (66.03%), Query Frame = 0

Query: 1   MGG-RVASCLI---LLLLFLFDEAS---AEVGEKSKQ------MEAESADNGVIKMVTKM 60
           MGG  + SC +   LLLL +F EA    +E+ EK+KQ      +EA      +IKMVTKM
Sbjct: 1   MGGTTMTSCFLLLPLLLLVMFGEARQTFSEIEEKNKQIELNDHIEATFKPKPIIKMVTKM 60

Query: 61  AYNSQELNSMADQQHHADDSSSSHMEHMNDPAQIFFTINDLKLGKKLPLYLAPKDPSTSP 120
           A          D   H   SSSSHM+H+N    +FFTINDLK GKKLP+Y   +DPS SP
Sbjct: 61  A---------NDHNSHDTHSSSSHMDHINPLLIVFFTINDLKQGKKLPIYFPKRDPSKSP 120

Query: 121 PMVAKEIADSIPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSM 180
           P++ KE AD IPFSL++LP+IL +FS+   S QA+ V+ TL QCE K ++GETKFC+TSM
Sbjct: 121 PLLPKEQADPIPFSLKKLPQILSYFSYPSNSPQAQAVKETLEQCELKPIKGETKFCATSM 180

Query: 181 ESMADFARLALGLASKSSLKHLVTTHLTKSRPPYLQNYTLLQMPN--AVTKFLACHLMTY 240
           ESM +F R A G   K+  + L T+HLTKS   +LQNYT ++ P   A  + +ACH M Y
Sbjct: 181 ESMVNFVRTAFG--PKTHYEVLTTSHLTKSN-VHLQNYTFIEAPKEIAAPRLVACHTMPY 240

Query: 241 PYAVHYCHYLHGGDKALQILLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVP 300
            YAV+YCHY  G +  L+I LEG+NG+ V A A+CHMDTS W+P H SF+VLK +PG +P
Sbjct: 241 AYAVYYCHYQEGDNNVLKIQLEGENGDRVEALAICHMDTSQWSPSHPSFQVLKIQPGTIP 300

BLAST of MS028232 vs. TAIR 10
Match: AT1G49320.1 (unknown seed protein like 1 )

HSP 1 Score: 172.2 bits (435), Expect = 6.5e-43
Identity = 98/258 (37.98%), Postives = 150/258 (58.14%), Query Frame = 0

Query: 48  AYNSQELNSMADQQHHADDSSSSHMEHMNDPA-QIFFTINDLKLGKKLPLYLAPKDPSTS 107
           A+ S++L S+ +++   D S        +DP+  ++FT+NDLKLG KL +Y    D    
Sbjct: 24  AHTSRKLISIKEKEGQ-DISHLLKDGEFDDPSLYMYFTLNDLKLGTKLLIYFYKNDLQKL 83

Query: 108 PPMVAKEIADSIPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGETKFCSTS 167
           PP++ ++ AD IPF+  +L  +L  FS +  S Q + ++ TL  C+ K +EGE KFC TS
Sbjct: 84  PPLLTRQQADLIPFTKSKLDFLLDHFSITKDSPQGKAIKETLGHCDAKAIEGEHKFCGTS 143

Query: 168 MESMADFARLALGLASKSSLKHLVTTHLTKSRPPY---LQNYTLLQMPNAVT--KFLACH 227
           +ES+ D  +  +G      LK + T  +  ++      L NYT ++ P  +   K L CH
Sbjct: 144 LESLIDLVKKTMGY--NVDLKVMTTKVMVPAQNSISYALHNYTFVEAPKELVGIKMLGCH 203

Query: 228 LMTYPYAVHYCHYLHGGDKALQILLEGDNGNV-VMAAAVCHMDTSGWTPDHVSFRVLKTK 287
            M YPYAV+YCH   GG +  ++ L  D+G   V+  AVCHMDTS W  DHV+F+VLK +
Sbjct: 204 RMPYPYAVYYCHGHKGGSRVFEVNLVTDDGRQRVVGPAVCHMDTSTWDADHVAFKVLKME 263

Query: 288 PGIVPICHIFPPNDLILI 299
           P   P+CH FP ++++ +
Sbjct: 264 PRSAPVCHFFPLDNIVWV 278

BLAST of MS028232 vs. TAIR 10
Match: AT5G25610.1 (BURP domain-containing protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.2e-33
Identity = 83/232 (35.78%), Postives = 127/232 (54.74%), Query Frame = 0

Query: 74  HMNDPAQIFFTINDLKLGKKLPLYLAPKDP-STSPPMVAKEIADSIPFSLEQLPEILRFF 133
           H +  A +FF   DL  GK++ +    +D        + +  A+++PF  E+  E L+ F
Sbjct: 168 HDDPNAALFFLEKDLVRGKEMNVRFNAEDGYGGKTAFLPRGEAETVPFGSEKFSETLKRF 227

Query: 134 SFSPGSRQARDVETTLHQCEHKRVEGETKFCSTSMESMADFARLALGLASKSSLKHLVTT 193
           S   GS +A  ++ T+ +CE ++V GE K+C+TS+ESM DF+   LG   K  ++  V+T
Sbjct: 228 SVEAGSEEAEMMKKTIEECEARKVSGEEKYCATSLESMVDFSVSKLG---KYHVR-AVST 287

Query: 194 HLTKSRPPYLQNYTLLQ---MPNAVTKFLACHLMTYPYAVHYCHYLHGGDKALQ-----I 253
            + K   P +Q Y +        +  K + CH   YP+AV YCH      KA+      +
Sbjct: 288 EVAKKNAP-MQKYKIAAAGVKKLSDDKSVVCHKQKYPFAVFYCH------KAMMTTVYAV 347

Query: 254 LLEGDNGNVVMAAAVCHMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPNDLI 297
            LEG+NG    A AVCH +TS W P+H++F+VLK KPG VP+CH  P   ++
Sbjct: 348 PLEGENGMRAKAVAVCHKNTSAWNPNHLAFKVLKVKPGTVPVCHFLPETHVV 388

BLAST of MS028232 vs. TAIR 10
Match: AT1G70370.1 (polygalacturonase 2 )

HSP 1 Score: 98.2 bits (243), Expect = 1.2e-20
Identity = 68/215 (31.63%), Postives = 96/215 (44.65%), Query Frame = 0

Query: 82  FFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIADSIPFSLEQLPEILRFFSFSPGSRQA 141
           FF  + LK G  +P+    KD       + + I   +PFS  +L EI R F     S   
Sbjct: 411 FFRESSLKEGTVIPM-PDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMG 470

Query: 142 RDVETTLHQCEHKRVEGETKFCSTSMESMADFARLALGLASKSSLKHLVTTHLTKSRPPY 201
             +   + +CE     GETK C  S E M DFA   LG     S+    T ++  S+   
Sbjct: 471 GIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLG----RSVVLRTTENVAGSKEKV 530

Query: 202 LQNYTLLQMPNAVTKFLACHLMTYPYAVHYCHYLHGGDKALQILLE-GDNGNVVMAAAVC 261
           +           +TK ++CH   YPY ++YCH +         LLE      +    A+C
Sbjct: 531 VIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAIC 590

Query: 262 HMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPNDL 296
           HMDTS W P H +F  L +KPG + +CH    ND+
Sbjct: 591 HMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDM 620

BLAST of MS028232 vs. TAIR 10
Match: AT1G70370.2 (polygalacturonase 2 )

HSP 1 Score: 98.2 bits (243), Expect = 1.2e-20
Identity = 68/215 (31.63%), Postives = 96/215 (44.65%), Query Frame = 0

Query: 82  FFTINDLKLGKKLPLYLAPKDPSTSPPMVAKEIADSIPFSLEQLPEILRFFSFSPGSRQA 141
           FF  + LK G  +P+    KD       + + I   +PFS  +L EI R F     S   
Sbjct: 411 FFRESSLKEGTVIPM-PDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMG 470

Query: 142 RDVETTLHQCEHKRVEGETKFCSTSMESMADFARLALGLASKSSLKHLVTTHLTKSRPPY 201
             +   + +CE     GETK C  S E M DFA   LG     S+    T ++  S+   
Sbjct: 471 GIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLG----RSVVLRTTENVAGSKEKV 530

Query: 202 LQNYTLLQMPNAVTKFLACHLMTYPYAVHYCHYLHGGDKALQILLE-GDNGNVVMAAAVC 261
           +           +TK ++CH   YPY ++YCH +         LLE      +    A+C
Sbjct: 531 VIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAIC 590

Query: 262 HMDTSGWTPDHVSFRVLKTKPGIVPICHIFPPNDL 296
           HMDTS W P H +F  L +KPG + +CH    ND+
Sbjct: 591 HMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDM 620

BLAST of MS028232 vs. TAIR 10
Match: AT1G60390.1 (polygalacturonase 1 )

HSP 1 Score: 92.0 bits (227), Expect = 8.5e-19
Identity = 58/196 (29.59%), Postives = 89/196 (45.41%), Query Frame = 0

Query: 101 KDPSTSPPMVAKEIADSIPFSLEQLPEILRFFSFSPGSRQARDVETTLHQCEHKRVEGET 160
           KD       + + I  ++PFS   + EI R F     S  A  + + + +CE     GET
Sbjct: 427 KDKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGET 486

Query: 161 KFCSTSMESMADFARLALGLASKSSLKHLVTTHLTKSRPPYLQNYTLLQMPNAVTKFLAC 220
           K C  S E M DFA   LG      +    T ++  S+   +           VT+ ++C
Sbjct: 487 KRCVGSAEDMIDFATSVLG----RGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSC 546

Query: 221 HLMTYPYAVHYCHYLHGGDKALQILLEGDN-GNVVMAAAVCHMDTSGWTPDHVSFRVLKT 280
           H   YPY ++YCH +         LL+  +   +    A+CH+DTS W+P H +F  L +
Sbjct: 547 HQSLYPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGS 606

Query: 281 KPGIVPICHIFPPNDL 296
            PG + +CH    ND+
Sbjct: 607 GPGQIEVCHWIFENDM 618