MS028212 (gene) Bitter gourd (TR) v1

BLAST of MS028212 vs. NCBI nr
Match: XP_038887395.1 (protein DMR6-LIKE OXYGENASE 2-like [Benincasa hispida])

HSP 1 Score: 456.1 bits (1172), Expect = 2.8e-124
Identity = 236/348 (67.82%), Postives = 263/348 (75.57%), Query Frame = 0

Query: 6   FDPAFILAPDHRPKLAAAEFADGIPTVDLS---------LPDGDLVRQVGSASRDWGFFL 65
           FD AFI AP+HRPKLA +E ADGIPT+DLS          PD DLVRQ+ SAS +WGFFL
Sbjct: 7   FDKAFIQAPEHRPKLAVSE-ADGIPTIDLSPIFNSPGGDFPD-DLVRQIASASSEWGFFL 66

Query: 66  VTNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWKEVFDL 125
           V NHGV  EKRRRIEA AR FF QSLEEKRKVRRDEG  TGY+DTELTKNVRDWKEVFD 
Sbjct: 67  VVNHGVPSEKRRRIEATAREFFGQSLEEKRKVRRDEGVVTGYFDTELTKNVRDWKEVFDF 126

Query: 126 TVADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALSLG 185
            VADPTVVPASPEP D ELTHWTNQWP YPPE REA EEYVEE+EKLGHKLMELIA+SLG
Sbjct: 127 MVADPTVVPASPEPDDDELTHWTNQWPEYPPEFREACEEYVEELEKLGHKLMELIAVSLG 186

Query: 186 LPANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEGWRVDSSQTRT---QFFRHQ 245
           LPA RFR YFK+QTSF+RLNHYP C SP L LG G  ++       +Q      +  R +
Sbjct: 187 LPAKRFRDYFKEQTSFVRLNHYPSCPSPELALGVGRHKDPSVLTVLAQDDVGGLEVKRKR 246

Query: 246 CGRYNSGGYLP----IISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFLNPSHDTIV 305
            G +     +P    +   +I  VWSNEKYESVEHR  VNS  ER+SI FF NPSH TI+
Sbjct: 247 DGEWVRVKPVPDSYVVNVGDITQVWSNEKYESVEHRAMVNSKKERYSIAFFFNPSHSTII 306

Query: 306 EPLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFKIS 338
           EPLEELI+PQNP KYK +++GKF  NRKRSNFKKL+ ENIQISHFKI+
Sbjct: 307 EPLEELINPQNPLKYKSFTYGKFLANRKRSNFKKLNVENIQISHFKIT 352

BLAST of MS028212 vs. NCBI nr
Match: XP_023554292.1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 442.6 bits (1137), Expect = 3.2e-120
Identity = 230/348 (66.09%), Postives = 259/348 (74.43%), Query Frame = 0

Query: 6   FDPAFILAPDHRPKLAAAEFADGIPTVDLS----LPDG----DLVRQVGSASRDWGFFLV 65
           FD AFI AP+HRPKL+  E ADGIPTVDLS     PDG    DLVRQ+ SAS DWGFF V
Sbjct: 4   FDQAFIQAPEHRPKLSDPE-ADGIPTVDLSPIFNSPDGTFPDDLVRQIASASADWGFFFV 63

Query: 66  TNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWKEVFDLT 125
            NHGV  EK+RRIEAAAR+FF QSLEEKRKVRRDEG  TGY +TELTKNVRDWKEVFD  
Sbjct: 64  VNHGVPPEKQRRIEAAARVFFGQSLEEKRKVRRDEGLVTGYSNTELTKNVRDWKEVFDFV 123

Query: 126 VADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALSLGL 185
           VADPTVVPASPEP D +LT WTN+WP Y PE R+A EEYVEE+EKLGHKLMEL+A+SLGL
Sbjct: 124 VADPTVVPASPEPDDDQLTLWTNRWPEYLPEFRKACEEYVEELEKLGHKLMELLAVSLGL 183

Query: 186 PANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEG----WRVDSSQTRTQFFRHQ 245
           PA RFR YFK+ TSF RLNHYPPC SP L LG G  ++ G       D      +  R +
Sbjct: 184 PAERFRDYFKETTSFGRLNHYPPCPSPELALGVGRHKDPGVLTVLAQDDEVEGLEVKRKR 243

Query: 246 CGRYNSGGYLP----IISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFLNPSHDTIV 305
            G +     +P    +   EI   WSNE+YESVEHR  VN   ER+SI FF NPSH TIV
Sbjct: 244 DGEWIRVKPVPDSYVVNVGEITQAWSNERYESVEHRAMVNHKRERYSIAFFFNPSHSTIV 303

Query: 306 EPLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFKIS 338
           EPL+ELIDP+NPPKYK YSWGK+ +NR  SNFKKL+A+NIQ+SHFKIS
Sbjct: 304 EPLKELIDPENPPKYKSYSWGKYLSNRMLSNFKKLNADNIQVSHFKIS 350

BLAST of MS028212 vs. NCBI nr
Match: KAG6572000.1 (putative 2-oxoglutarate-dependent dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 440.3 bits (1131), Expect = 1.6e-119
Identity = 229/348 (65.80%), Postives = 257/348 (73.85%), Query Frame = 0

Query: 6   FDPAFILAPDHRPKLAAAEFADGIPTVDLS----LPDG----DLVRQVGSASRDWGFFLV 65
           FD AFI AP+HRPKL+  E ADGIPTVDLS     PDG    DLVRQ+ SAS DWGFF V
Sbjct: 4   FDQAFIQAPEHRPKLSDPE-ADGIPTVDLSPIFNSPDGTFPDDLVRQIASASADWGFFFV 63

Query: 66  TNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWKEVFDLT 125
            NHGV  EK+RRIEAAAR FF QSLEEKRKVRRDEG  TGY +TELTKNVRDWKEVFD  
Sbjct: 64  VNHGVPPEKQRRIEAAAREFFGQSLEEKRKVRRDEGVVTGYSNTELTKNVRDWKEVFDFV 123

Query: 126 VADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALSLGL 185
           VADPTVVPASPEP D +LT WTN+WP Y PE R+A EEYVEE+EKLGHKLMEL+A+SLGL
Sbjct: 124 VADPTVVPASPEPDDDQLTKWTNRWPEYLPEFRKACEEYVEELEKLGHKLMELLAVSLGL 183

Query: 186 PANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEG----WRVDSSQTRTQFFRHQ 245
           PA RFR YFK+ TSF RLNHYPPC SP L LG G  ++ G       D      +  R +
Sbjct: 184 PAERFRDYFKETTSFGRLNHYPPCPSPELALGVGRHKDPGVLTVLAQDDDVEGLEVKRKR 243

Query: 246 CGRYNSGGYLP----IISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFLNPSHDTIV 305
            G +     +P    +   EI   WSNE+YESVEHR  VN   ER+SI FF NPSH TIV
Sbjct: 244 DGEWIRVKPVPDSYVVNVGEITQAWSNERYESVEHRAMVNHKRERYSIAFFFNPSHSTIV 303

Query: 306 EPLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFKIS 338
           EPL+ELIDP+NPPKYK YSWGK+ +NR  SNFKKL+ +NIQ+SHFKIS
Sbjct: 304 EPLKELIDPENPPKYKSYSWGKYLSNRMLSNFKKLNVDNIQVSHFKIS 350

BLAST of MS028212 vs. NCBI nr
Match: XP_022953056.1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita moschata])

HSP 1 Score: 440.3 bits (1131), Expect = 1.6e-119
Identity = 229/348 (65.80%), Postives = 257/348 (73.85%), Query Frame = 0

Query: 6   FDPAFILAPDHRPKLAAAEFADGIPTVDLS----LPDG----DLVRQVGSASRDWGFFLV 65
           FD AFI AP+HRPKL+ AE ADGIPTVDLS     PDG    DLVRQ+ SAS DWGFF V
Sbjct: 4   FDQAFIQAPEHRPKLSDAE-ADGIPTVDLSPIFNSPDGTFPDDLVRQIASASADWGFFFV 63

Query: 66  TNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWKEVFDLT 125
            NHGV  EK+RRIEAAAR FF QSLEEKRKVRRDEG  TGY +TELTKNVRDWKEVFD  
Sbjct: 64  VNHGVPPEKQRRIEAAAREFFGQSLEEKRKVRRDEGVVTGYSNTELTKNVRDWKEVFDFV 123

Query: 126 VADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALSLGL 185
           VADPTVVPASPEP D +L  WTN+WP Y PE R+A EEYVEE+EKLGHKLMEL+A+SLGL
Sbjct: 124 VADPTVVPASPEPDDDQLAKWTNRWPEYLPEFRKACEEYVEELEKLGHKLMELLAVSLGL 183

Query: 186 PANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEG----WRVDSSQTRTQFFRHQ 245
           PA RFR YFK+ TSF RLNHYPPC SP L LG G  ++ G       D      +  R +
Sbjct: 184 PAERFRDYFKETTSFGRLNHYPPCPSPELALGVGRHKDPGVLTVLAQDDDVEGLEVKRKR 243

Query: 246 CGRYNSGGYLP----IISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFLNPSHDTIV 305
            G +     +P    +   EI   WSNE+YESVEHR  VN   ER+SI FF NPSH TIV
Sbjct: 244 DGEWIRVKPVPDSYVVNVGEITQAWSNERYESVEHRAMVNHKRERYSIAFFFNPSHSTIV 303

Query: 306 EPLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFKIS 338
           EPL+ELIDP+NPPKYK YSWGK+ +NR  SNFKKL+ +NIQ+SHFKIS
Sbjct: 304 EPLKELIDPENPPKYKSYSWGKYLSNRMLSNFKKLNVDNIQVSHFKIS 350

BLAST of MS028212 vs. NCBI nr
Match: XP_011658920.1 (protein DMR6-LIKE OXYGENASE 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 435.3 bits (1118), Expect = 5.2e-118
Identity = 230/353 (65.16%), Postives = 259/353 (73.37%), Query Frame = 0

Query: 1   MADLQFDPAFILAPDHRPKLAAAEFADGIPTVDL-----SLPDG----DLVRQVGSASRD 60
           M DL+    FI AP+HRPKLA +  ADGIPT+DL     SLP      DLV Q+ SA  +
Sbjct: 1   MGDLE---DFIQAPEHRPKLALSH-ADGIPTIDLSPIFNSLPGSDFPHDLVHQIASACTE 60

Query: 61  WGFFLVTNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWK 120
           WGFFLV NHGV  EKR RIEAAAR FF QSLEEKRKVRR EG  TGY+D+ELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSEGLVTGYFDSELTKNVRDWK 120

Query: 121 EVFDLTVADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELI 180
           EVFDL V DPT+VPASPE  D ELT WTNQWP YPPE RE+ +EYVEE+EKLGHKLMEL+
Sbjct: 121 EVFDLVVEDPTIVPASPESDDEELTQWTNQWPEYPPEFRESCKEYVEELEKLGHKLMELL 180

Query: 181 ALSLGLPANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEGWRVDSSQTRT---Q 240
           ALSLGL A RF+ YFK QTSF+R+NHYPPC SP LTLG G  ++ G     +Q      +
Sbjct: 181 ALSLGLSAKRFQHYFKQQTSFLRINHYPPCPSPELTLGVGRHKDPGVLTVLAQDDVGGLE 240

Query: 241 FFRHQCGRYNSGGYLP----IISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFLNPS 300
             R + G +     +P    +   EI  VWSNEKYESVEHR TVNS  +R+SI FF  PS
Sbjct: 241 VKRKRDGEWIRVKPVPDSFVVNIGEITQVWSNEKYESVEHRATVNSKRDRYSIAFFFYPS 300

Query: 301 HDTIVEPLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFKIS 338
           H TIVEPLEELI PQNPPKYKPYS+GKF  NRKRSNFKKL+ +NIQIS FKIS
Sbjct: 301 HSTIVEPLEELIGPQNPPKYKPYSFGKFLANRKRSNFKKLNVDNIQISDFKIS 349

BLAST of MS028212 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.5e-32
Identity = 106/310 (34.19%), Postives = 143/310 (46.13%), Query Frame = 0

Query: 29  IPTVDLS----LPDGDLVRQVGSASRDWGFFLVTNHGVALEKRRRIEAAARMFFRQSLEE 88
           IP +D++     P+G  +R V SA  +WGFF + NHGV      R+  A R FF   LEE
Sbjct: 48  IPVLDMNDVWGKPEG--LRLVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEE 107

Query: 89  KRKVRRDEGRSTGYYD-TELTKNVR-DWKEVFDLTVADPTVVPASPEPHDGELTHWTNQW 148
           KRK         GY     + K+ + DW + F L     ++   S             +W
Sbjct: 108 KRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNYLPSSIRNPS-------------KW 167

Query: 149 PAYPPELREAFEEYVEEVEKLGHKLMELIALSLGLPANRFR---GYFKDQTSFIRLNHYP 208
           P+ PP++RE  E+Y EEV KL  +L E ++ SLGL  N+     G      + +R N YP
Sbjct: 168 PSQPPKIRELIEKYGEEVRKLCERLTETLSESLGLKPNKLMQALGGGDKVGASLRTNFYP 227

Query: 209 PCSSPHLTLGAGSEEEEGW--------RVDSSQTRTQFFRHQCGRYNSGGYLPIIS-PEI 268
            C  P LTLG  S  + G         +V   Q R              G++ I S P  
Sbjct: 228 KCPQPQLTLGLSSHSDPGGITILLPDEKVAGLQVR-----------RGDGWVTIKSVPNA 287

Query: 269 LCV--------WSNEKYESVEHRVTVNSTSERFSIPFFLNPSHDTIVEPLEELIDPQNPP 313
           L V         SN  Y+SVEH+V VNS  ER S+ FF NP  D  V P+EEL+    P 
Sbjct: 288 LIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSDIPVGPIEELVTANRPA 331

BLAST of MS028212 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 2.6e-32
Identity = 108/335 (32.24%), Postives = 153/335 (45.67%), Query Frame = 0

Query: 10  FILAPDHRPKLAAAEFADGIPTVD----LSLPDG--DLVRQVGSASRDWGFFLVTNHGVA 69
           FI   D +P +   E    +P +D    LS P    D  R +  A +  GFFLV NHG++
Sbjct: 44  FIWPDDEKPSINVLEL--DVPLIDLQNLLSDPSSTLDASRLISEACKKHGFFLVVNHGIS 103

Query: 70  LEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYD--TELTKNVRDWKEVFDLTVADP 129
            E           FF   L EK++V R  G S GY    T        WKE       D 
Sbjct: 104 EELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDD 163

Query: 130 TVVPASPEPHDGE-LTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALSLGLPAN 189
                S + +  + L H       + P   + ++EY E +  L  K+MEL+ LSLG+  +
Sbjct: 164 MSRSKSVQDYFCDALGH------GFQP-FGKVYQEYCEAMSSLSLKIMELLGLSLGVKRD 223

Query: 190 RFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEGWRVDSSQTRTQ----FFRHQCG- 249
            FR +F++  S +RLN+YPPC  P LTLG G   +        Q        F  +Q   
Sbjct: 224 YFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRS 283

Query: 250 -RYNSGGYLPIISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFLNPSHDTIVEPLEE 309
            R N   ++  I    + + SN++Y+S  HR  VNS SER S+ FFL P  D +V P  E
Sbjct: 284 IRPNPKAFVVNIGDTFMAL-SNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRE 343

Query: 310 LIDPQNPPKYKPYSWGKF--FTNRK-RSNFKKLDA 327
           L+D     +Y  ++W  F  FT +  R++   L A
Sbjct: 344 LLDSITSRRYPDFTWSMFLEFTQKHYRADMNTLQA 368

BLAST of MS028212 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.3e-31
Identity = 95/309 (30.74%), Postives = 145/309 (46.93%), Query Frame = 0

Query: 23  AEFADGIPTVDL-------SLPDGDLVRQVGSASRDWGFFLVTNHGVALEKRRRIEAAAR 82
           A  A  IP +DL        L D  ++ ++  A R WGFF V NHGV  E         R
Sbjct: 56  APTATNIPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWR 115

Query: 83  MFFRQSLEEKRKVRRDEGRSTGY---YDTELTKNVRDWKEVFDLTVADPTVVPASPEPHD 142
            FF   +  K           GY      E   ++ DW + + L +           PH 
Sbjct: 116 EFFHMPVNAKETYSNSPRTYEGYGSRLGVEKGASL-DWSDYYFLHLL----------PH- 175

Query: 143 GELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALSLGLPANRFRGYFKDQT-- 202
             L  + N+WP++PP +RE  +EY EE+ KL  ++M +++ +LGL  ++F+  F  +   
Sbjct: 176 -HLKDF-NKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIG 235

Query: 203 SFIRLNHYPPCSSPHLTLGAGSEEEEGWRV----DSSQTRTQFFRHQCG---RYNSGGYL 262
           + +R+N+YP C  P L LG     + G       D      Q  +       + +   ++
Sbjct: 236 ACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFI 295

Query: 263 PIISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFLNPSHDTIVEPLEELIDPQNPPK 313
             I  +I  + SN  Y+SVEHRV VNS  ER S+ FF NP  D  ++PL+EL+   NPP 
Sbjct: 296 VNIGDQIQ-ILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPL 349

BLAST of MS028212 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 5.0e-31
Identity = 96/294 (32.65%), Postives = 139/294 (47.28%), Query Frame = 0

Query: 26  ADGIPTVDLSLPDGD-LVRQVGSASRDWGFFLVTNHGVALEKRRRIEAAARMFFRQSLEE 85
           A  IPT+DL  P  D LVR +  ASR+WG F VTNHG+  +   +++A  + FF    EE
Sbjct: 40  APEIPTIDLDDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLICKLQAVGKEFFELPQEE 99

Query: 86  KRKVRRD-EGRSTGYYDTELTKNV---RDWKEVFDLTVADPTVVPASPEPHDGELTHWTN 145
           K    R  + +    Y T+L K V   + W +     V  P+ +                
Sbjct: 100 KEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSIN-------------YR 159

Query: 146 QWPAYPPELREAFEEYVEEVEKLGHKLMELIALSLGLPANRFR--GYFKDQTSFIRLNHY 205
            WP  PP  R   EEY + + ++  KL   ++L LG+     +      D    +++N+Y
Sbjct: 160 FWPKNPPSYRAVNEEYAKYMREVVDKLFTYLSLGLGVEGGVLKEAAGGDDIEYMLKINYY 219

Query: 206 PPCSSPHLTLGAGSEEEEG---WRVDSSQTRTQFFRHQCGRYNSGGYLP----IISPEIL 265
           PPC  P L LG  +  +       V +     Q F+    R+    Y+P    I   + +
Sbjct: 220 PPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFKDD--RWIDAKYIPNALVIHIGDQI 279

Query: 266 CVWSNEKYESVEHRVTVNSTSERFSIPFFLNPSHDTIVEPLEELIDPQNPPKYK 306
            + SN KY++V HR TVN    R S P FL P  DT+V PL +L+D +NPPKYK
Sbjct: 280 EILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADTVVGPLPQLVDDENPPKYK 318

BLAST of MS028212 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.9e-30
Identity = 99/335 (29.55%), Postives = 151/335 (45.07%), Query Frame = 0

Query: 5   QFDPAFILAPDHRPKLAAAEFADGIPTVDLSLPDGDL--VRQVGSASRDWGFFLVTNHGV 64
           Q    FI   + +P +   E    +P +DLS  D  L   R +  A    GFFLV NHGV
Sbjct: 41  QIPNQFIWPDEEKPSIDIPEL--NVPFIDLSSQDSTLEAPRVIAEACTKHGFFLVVNHGV 100

Query: 65  ALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYD--TELTKNVRDWKEVFDLTVAD 124
           +             FF   L  K+K +R  G S GY    T        WKE      ++
Sbjct: 101 SESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSN 160

Query: 125 PTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALSLGLPAN 184
                 + + +  +      +      +  + +++Y E +  L  K+MEL+ LSLG+  +
Sbjct: 161 DNSGSRTVQDYFSDTLGQEFE------QFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRD 220

Query: 185 RFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEGWRVDSSQTRT---QFF---RHQC 244
            FRG+F++  S +RLNHYPPC +P LTLG G   +        Q      Q F   + Q 
Sbjct: 221 YFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQS 280

Query: 245 GRYNSGGYLPIISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFLNPSHDTIVEPLEE 304
            R N   ++  I    + + SN  ++S  HR  VN  S R S+ FFL P  D +V+P  +
Sbjct: 281 IRPNPKAFVVNIGDTFMAL-SNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSD 340

Query: 305 LIDPQNPPKYKPYSWGKF--FTNRK-RSNFKKLDA 327
           +++     KY  ++W  F  FT +  R++   LD+
Sbjct: 341 ILEKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDS 366

BLAST of MS028212 vs. ExPASy TrEMBL
Match: A0A6J1GLY0 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX=3662 GN=LOC111455576 PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 7.8e-120
Identity = 229/348 (65.80%), Postives = 257/348 (73.85%), Query Frame = 0

Query: 6   FDPAFILAPDHRPKLAAAEFADGIPTVDLS----LPDG----DLVRQVGSASRDWGFFLV 65
           FD AFI AP+HRPKL+ AE ADGIPTVDLS     PDG    DLVRQ+ SAS DWGFF V
Sbjct: 4   FDQAFIQAPEHRPKLSDAE-ADGIPTVDLSPIFNSPDGTFPDDLVRQIASASADWGFFFV 63

Query: 66  TNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWKEVFDLT 125
            NHGV  EK+RRIEAAAR FF QSLEEKRKVRRDEG  TGY +TELTKNVRDWKEVFD  
Sbjct: 64  VNHGVPPEKQRRIEAAAREFFGQSLEEKRKVRRDEGVVTGYSNTELTKNVRDWKEVFDFV 123

Query: 126 VADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALSLGL 185
           VADPTVVPASPEP D +L  WTN+WP Y PE R+A EEYVEE+EKLGHKLMEL+A+SLGL
Sbjct: 124 VADPTVVPASPEPDDDQLAKWTNRWPEYLPEFRKACEEYVEELEKLGHKLMELLAVSLGL 183

Query: 186 PANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEG----WRVDSSQTRTQFFRHQ 245
           PA RFR YFK+ TSF RLNHYPPC SP L LG G  ++ G       D      +  R +
Sbjct: 184 PAERFRDYFKETTSFGRLNHYPPCPSPELALGVGRHKDPGVLTVLAQDDDVEGLEVKRKR 243

Query: 246 CGRYNSGGYLP----IISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFLNPSHDTIV 305
            G +     +P    +   EI   WSNE+YESVEHR  VN   ER+SI FF NPSH TIV
Sbjct: 244 DGEWIRVKPVPDSYVVNVGEITQAWSNERYESVEHRAMVNHKRERYSIAFFFNPSHSTIV 303

Query: 306 EPLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFKIS 338
           EPL+ELIDP+NPPKYK YSWGK+ +NR  SNFKKL+ +NIQ+SHFKIS
Sbjct: 304 EPLKELIDPENPPKYKSYSWGKYLSNRMLSNFKKLNVDNIQVSHFKIS 350

BLAST of MS028212 vs. ExPASy TrEMBL
Match: A0A0A0K3A9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G074960 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 2.5e-118
Identity = 228/353 (64.59%), Postives = 261/353 (73.94%), Query Frame = 0

Query: 1   MADLQFDPAFILAPDHRPKLAAAEFADGIPTVDL-----SLPDG----DLVRQVGSASRD 60
           M DL+    FI AP+HRPKLA +  ADGIPT+DL     SLP      DLV Q+ SA  +
Sbjct: 1   MGDLE---DFIQAPEHRPKLALSH-ADGIPTIDLSPIFNSLPGSDFPHDLVHQIASACTE 60

Query: 61  WGFFLVTNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWK 120
           WGFFLV NHGV  EKR RIEAAAR FF QSLEEKRKVRR EG  TGY+D+ELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSEGLVTGYFDSELTKNVRDWK 120

Query: 121 EVFDLTVADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELI 180
           EVFDL V DPT+VPASPE  D ELT WTNQWP YPPE RE+ +EYVEE+EKLGHKLMEL+
Sbjct: 121 EVFDLVVEDPTIVPASPESDDEELTQWTNQWPEYPPEFRESCKEYVEELEKLGHKLMELL 180

Query: 181 ALSLGLPANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEGWRVDSSQTRT---Q 240
           ALSLGL A RF+ YFK QTSF+R+NHYPPC SP LTLG G  ++ G     +Q      +
Sbjct: 181 ALSLGLSAKRFQHYFKQQTSFLRINHYPPCPSPELTLGVGRHKDPGVLTVLAQDDVGGLE 240

Query: 241 FFRHQCGRYNSGGYLP----IISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFLNPS 300
             R + G +     +P    +   EI  VWSNEKYESVEHRV VNS  +R+SIPFF NPS
Sbjct: 241 VKRKRDGEWIRVKPVPDSFVVNIGEITQVWSNEKYESVEHRVMVNSEKDRYSIPFFFNPS 300

Query: 301 HDTIVEPLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFKIS 338
           H TIVEPL+EL+D QNPPKYK YS+GKF TNR+RSNFKKL+ +NIQIS FKI+
Sbjct: 301 HSTIVEPLKELVDSQNPPKYKSYSYGKFLTNRQRSNFKKLNTDNIQISDFKIT 349

BLAST of MS028212 vs. ExPASy TrEMBL
Match: A0A1S3BZR3 (LOW QUALITY PROTEIN: protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo OX=3656 GN=LOC103495207 PE=3 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 6.2e-117
Identity = 224/356 (62.92%), Postives = 261/356 (73.31%), Query Frame = 0

Query: 1   MADL-QFDPAFILAPDHRPKLAAAEFADGIPTVDLS----------LPDGDLVRQVGSAS 60
           M DL +FD AF+ +P+HRPKLA ++ AD IPT+DLS           PD DLV+Q+ +A 
Sbjct: 1   MGDLEEFDQAFVQSPEHRPKLALSD-ADEIPTIDLSPIFNSVPGSDFPD-DLVQQIAAAC 60

Query: 61  RDWGFFLVTNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRD 120
            +WGFFLV NHGV  EKR RIEAAAR FF QSLEEKRKVRR EG  TGY+DTELTKNVRD
Sbjct: 61  TEWGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSEGMVTGYFDTELTKNVRD 120

Query: 121 WKEVFDLTVADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLME 180
           WKEVFDL VADPT+VPASPEP D ELT WTNQWP YPPE R+   E+ EE+EKLGHKLM+
Sbjct: 121 WKEVFDLVVADPTIVPASPEPDDEELTQWTNQWPEYPPEFRKTCMEFAEELEKLGHKLMQ 180

Query: 181 LIALSLGLPANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEGWRVDSSQTRT-- 240
           LIAL LGL   RFR YFK+QTSF+RLNHYP C SP L LG G  ++ G     +Q     
Sbjct: 181 LIALXLGLSPKRFRDYFKEQTSFLRLNHYPSCPSPELILGVGRHKDPGVLTVLAQDDVGG 240

Query: 241 -QFFRHQCGRY-----NSGGYLPIISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFL 300
            +  R + G +         Y+  +  EI  VWSNE+YESVEHRV VNS  +R+SIPFF 
Sbjct: 241 LEVKRKRDGEWIRVKPEPDSYVVNLG-EITQVWSNERYESVEHRVMVNSEKDRYSIPFFF 300

Query: 301 NPSHDTIVEPLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFKIS 338
           NPSH TIVEPL+EL+D QNPPKYK YS+GKF TNR+RSNFKKL+ +NIQIS FKI+
Sbjct: 301 NPSHSTIVEPLKELVDSQNPPKYKSYSYGKFLTNRQRSNFKKLNTDNIQISDFKIT 353

BLAST of MS028212 vs. ExPASy TrEMBL
Match: A0A6J1I8C6 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470930 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 3.1e-116
Identity = 221/348 (63.51%), Postives = 252/348 (72.41%), Query Frame = 0

Query: 6   FDPAFILAPDHRPKLAAAEFADGIPTVDLS----LPDG----DLVRQVGSASRDWGFFLV 65
           FD AFI AP+HRPKL+  E ADGIPTVDLS     PDG    DLVRQ+ S S DWGFFLV
Sbjct: 4   FDQAFIQAPEHRPKLSDPE-ADGIPTVDLSPIFNSPDGTFPDDLVRQIASTSIDWGFFLV 63

Query: 66  TNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWKEVFDLT 125
            NHGV  EKR+R+EAA+R FFR+SLEEKR V + EG  TGY D ELTKNVRDWKEVFD  
Sbjct: 64  VNHGVPAEKRQRMEAASREFFRRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFDFI 123

Query: 126 VADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALSLGL 185
           VADPTVVP SPEP+D  LTHWTNQWP Y PE R A EEYVEE+EKLGHKLMEL+A+SLGL
Sbjct: 124 VADPTVVPVSPEPNDHRLTHWTNQWPTYLPEFRRACEEYVEELEKLGHKLMELLAVSLGL 183

Query: 186 PANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEG----WRVDSSQTRTQFFRHQ 245
           PA RFR YFK+ TSF RLNHYPPC SP L LG G  ++ G       D      +  R +
Sbjct: 184 PAERFRDYFKETTSFGRLNHYPPCPSPELALGVGRHKDPGVLTVLAQDDDVEGLEVKRKR 243

Query: 246 CGRYNSGGYLP----IISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFLNPSHDTIV 305
            G +     +P    +   EI   WSNE+YESVEHR  VN   ER+SI FF NPSH T+V
Sbjct: 244 DGEWIRVKPVPDSYVVNVGEITQAWSNERYESVEHRAMVNHKKERYSIAFFFNPSHSTVV 303

Query: 306 EPLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFKIS 338
           EPL+ELI P+NPP YK YSWGK+ +NR  SNFK+L+A+NIQ+SHFKIS
Sbjct: 304 EPLKELITPENPPNYKSYSWGKYLSNRMLSNFKQLNADNIQVSHFKIS 350

BLAST of MS028212 vs. ExPASy TrEMBL
Match: A0A6J1I5R5 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470930 PE=3 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 9.8e-115
Identity = 216/347 (62.25%), Postives = 251/347 (72.33%), Query Frame = 0

Query: 6   FDPAFILAPDHRPKLAAAEFADGIPTVDLS----LPDG----DLVRQVGSASRDWGFFLV 65
           FD AFI AP+HRPKL+  E ADGIPTVDLS     PDG    DLVRQ+ S S DWGFFLV
Sbjct: 4   FDQAFIQAPEHRPKLSDPE-ADGIPTVDLSPIFNSPDGTFPDDLVRQIASTSIDWGFFLV 63

Query: 66  TNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWKEVFDLT 125
            NHGV  EKR+R+EAA+R FFR+SLEEKR V + EG  TGY D ELTKNVRDWKEVFD  
Sbjct: 64  VNHGVPAEKRQRMEAASREFFRRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFDFI 123

Query: 126 VADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALSLGL 185
           VADPTVVP SPEP+D  LTHWTNQWP Y PE REA +EY EE+EKLGHKL+ELIA+SLGL
Sbjct: 124 VADPTVVPVSPEPNDHRLTHWTNQWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSLGL 183

Query: 186 PANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEG---WRVDSSQTRTQFFRHQC 245
           PA RFR YFK++TS +RLNHYPPCS P + LG G   + G     V       +  R + 
Sbjct: 184 PAQRFRDYFKEKTSMVRLNHYPPCSLPEMALGVGRHNDPGVLTMLVQDDVGGLEVKRKRD 243

Query: 246 GRYNSGGYLP----IISPEILCVWSNEKYESVEHRVTVNSTSERFSIPFFLNPSHDTIVE 305
           G +     +P    +    +   WSNE+YESVEHR  VN   ER+SI FF NPSH T+VE
Sbjct: 244 GEWIRVKPVPDSYVVNIGNVTQAWSNERYESVEHRAMVNHKKERYSIAFFFNPSHSTVVE 303

Query: 306 PLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFKIS 338
           PL+ELI P+NPP YK YSWGK+ +NR  SNFK+L+A+NIQ+SHFKIS
Sbjct: 304 PLKELITPENPPNYKSYSWGKYLSNRMLSNFKQLNADNIQVSHFKIS 349

BLAST of MS028212 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.9e-94
Identity = 175/351 (49.86%), Postives = 224/351 (63.82%), Query Frame = 0

Query: 5   QFDPAFILAPDHRPKLAAAE-----FADGIPTVDL-SLPDGD-----LVRQVGSASRDWG 64
           + D AFI AP+HRP           F+D IPT+DL SL D       + +++  A + WG
Sbjct: 3   ELDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWG 62

Query: 65  FFLVTNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWKEV 124
           FF V NHG+    R R+E  A  FF  + EEKRKV+RDE    GY+D E TKNVRDWKE+
Sbjct: 63  FFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEI 122

Query: 125 FDLTVADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIAL 184
           FD  + D T+VPASPEP D EL   TNQWP  P   RE  +EY  EVEKL  +L+EL+++
Sbjct: 123 FDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSI 182

Query: 185 SLGLPANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEGWRVDSSQTRTQFFRHQ 244
           SLGLP +R  G+F +QTSF+R NHYPPC +P L LG G  ++ G     +Q        Q
Sbjct: 183 SLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGL--Q 242

Query: 245 CGRYNSGGYLPI--ISPEILC-------VWSNEKYESVEHRVTVNSTSERFSIPFFLNPS 304
             R + G ++P+  IS  ++        VW+N++Y S EHRV VN++ ERFSIPFF  PS
Sbjct: 243 VSRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFFFPS 302

Query: 305 HDTIVEPLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFK 336
           H+  +EPLEELI  +NPP YK Y+WGKFF +R RS+FKKL+ ENIQI HFK
Sbjct: 303 HEANIEPLEELISEENPPCYKKYNWGKFFVSRNRSDFKKLEVENIQIDHFK 351

BLAST of MS028212 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 320.9 bits (821), Expect = 1.3e-87
Identity = 169/353 (47.88%), Postives = 217/353 (61.47%), Query Frame = 0

Query: 1   MADLQFDPAFILAPDHR-PKLAAAEFADGIPTVDLSLPDG-----DLVRQVGSASRDWGF 60
           M DL  DP +I AP+HR          + IP +DLS  D      +++ ++G A   WGF
Sbjct: 1   MEDL--DPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQNVISEIGDACEKWGF 60

Query: 61  FLVTNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWKEVF 120
           F V NHGV  + R+R+E   +MFF   +EEK KV+RD+    GY+D E TKNV+DWKEVF
Sbjct: 61  FQVLNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVF 120

Query: 121 DLTVADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALS 180
           D+   DP V+P++ +P D  L    N+WP  P + REA E Y    EKL  KL+ELI+LS
Sbjct: 121 DIYFKDPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLS 180

Query: 181 LGLPANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEGWRVDSSQTRTQFFRHQC 240
           LGLP  RF  YFK+Q SF R+N YPPC  P L LG G  ++    V S   +      Q 
Sbjct: 181 LGLPKERFHDYFKEQMSFFRINRYPPCPRPDLALGVGHHKDAD--VISLLAQDDVGGLQV 240

Query: 241 GRYNSGGYLPIIS-PEILC--------VWSNEKYESVEHRVTVNSTSERFSIPFFLNPSH 300
            R + G + PI   P  L         +W+N+KY S EHRV VN+T ER+SIPFFL PSH
Sbjct: 241 SRRSDGVWFPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSH 300

Query: 301 DTIVEPLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFKIST 339
           D  V+PLEEL+ P+NPPKYK Y WGKF+ +R RS+F+KL+ ENIQI  FK+ T
Sbjct: 301 DVEVKPLEELVSPENPPKYKGYKWGKFYVSRNRSDFRKLEIENIQIDDFKVKT 349

BLAST of MS028212 vs. TAIR 10
Match: AT3G19010.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 265.0 bits (676), Expect = 8.6e-71
Identity = 149/353 (42.21%), Postives = 194/353 (54.96%), Query Frame = 0

Query: 1   MADLQFDPAFILAPDHR-PKLAAAEFADGIPTVDLSLPDG-----DLVRQVGSASRDWGF 60
           M DL  DP +I AP+HR          + IP +DLS  D      +++ ++G A   WGF
Sbjct: 1   MEDL--DPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQNVISEIGDACEKWGF 60

Query: 61  FLVTNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWKEVF 120
           F V NHGV  + R+R+E   +MFF   +EEK KV+RD+    GY+D E TKNV+DWKEVF
Sbjct: 61  FQVLNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVF 120

Query: 121 DLTVADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALS 180
           D+   DP V+P++ +P D  L    N+WP  P + REA E Y    EKL           
Sbjct: 121 DIYFKDPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLA---------- 180

Query: 181 LGLPANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEGWRVDSSQTRTQFFRHQC 240
                               +N YPPC  P L LG G  ++    V S   +      Q 
Sbjct: 181 --------------------INRYPPCPRPDLALGVGHHKDAD--VISLLAQDDVGGLQV 240

Query: 241 GRYNSGGYLPIIS-PEILC--------VWSNEKYESVEHRVTVNSTSERFSIPFFLNPSH 300
            R + G + PI   P  L         +W+N+KY S EHRV VN+T ER+SIPFFL PSH
Sbjct: 241 SRRSDGVWFPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSH 300

Query: 301 DTIVEPLEELIDPQNPPKYKPYSWGKFFTNRKRSNFKKLDAENIQISHFKIST 339
           D  V+PLEEL+ P+NPPKYK Y WGKF+ +R RS+F+KL+ ENIQI  FK+ T
Sbjct: 301 DVEVKPLEELVSPENPPKYKGYKWGKFYVSRNRSDFRKLEIENIQIDDFKVKT 319

BLAST of MS028212 vs. TAIR 10
Match: AT3G19000.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.6e-61
Identity = 124/278 (44.60%), Postives = 164/278 (58.99%), Query Frame = 0

Query: 5   QFDPAFILAPDHRPKLAAAE-----FADGIPTVDL-SLPDGD-----LVRQVGSASRDWG 64
           + D AFI AP+HRP           F+D IPT+DL SL D       + +++  A + WG
Sbjct: 3   ELDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWG 62

Query: 65  FFLVTNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWKEV 124
           FF V NHG+    R R+E  A  FF  + EEKRKV+RDE    GY+D E TKNVRDWKE+
Sbjct: 63  FFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEI 122

Query: 125 FDLTVADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIAL 184
           FD  + D T+VPASPEP D EL   TNQWP  P   RE  +EY  EVEKL  +L+EL+++
Sbjct: 123 FDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSI 182

Query: 185 SLGLPANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEGWRVDSSQTRTQFFRHQ 244
           SLGLP +R  G+F +QTSF+R NHYPPC +P L LG G  ++ G     +Q        Q
Sbjct: 183 SLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGL--Q 242

Query: 245 CGRYNSGGYLPI--ISPEILCVWSNEKYESVEHRVTVN 270
             R + G ++P+  IS  ++    N    +V+ R+  N
Sbjct: 243 VSRRSDGQWIPVKPISDALIINMGNCIQVNVDFRLKTN 278

BLAST of MS028212 vs. TAIR 10
Match: AT3G19010.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.1e-52
Identity = 111/251 (44.22%), Postives = 145/251 (57.77%), Query Frame = 0

Query: 1   MADLQFDPAFILAPDHR-PKLAAAEFADGIPTVDLSLPDG-----DLVRQVGSASRDWGF 60
           M DL  DP +I AP+HR          + IP +DLS  D      +++ ++G A   WGF
Sbjct: 1   MEDL--DPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQNVISEIGDACEKWGF 60

Query: 61  FLVTNHGVALEKRRRIEAAARMFFRQSLEEKRKVRRDEGRSTGYYDTELTKNVRDWKEVF 120
           F V NHGV  + R+R+E   +MFF   +EEK KV+RD+    GY+D E TKNV+DWKEVF
Sbjct: 61  FQVLNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVF 120

Query: 121 DLTVADPTVVPASPEPHDGELTHWTNQWPAYPPELREAFEEYVEEVEKLGHKLMELIALS 180
           D+   DP V+P++ +P D  L    N+WP  P + REA E Y    EKL  KL+ELI+LS
Sbjct: 121 DIYFKDPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLS 180

Query: 181 LGLPANRFRGYFKDQTSFIRLNHYPPCSSPHLTLGAGSEEEEGWRVDSSQTRTQFFRHQC 240
           LGLP  RF  YFK+Q SF R+N YPPC  P L LG G  ++    V S   +      Q 
Sbjct: 181 LGLPKERFHDYFKEQMSFFRINRYPPCPRPDLALGVGHHKDAD--VISLLAQDDVGGLQV 240

Query: 241 GRYNSGGYLPI 246
            R + G + PI
Sbjct: 241 SRRSDGVWFPI 247