MS028182 (gene) Bitter gourd (TR) v1

Overview


Name	MS028182
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	mitogen-activated protein kinase kinase 9-like
Location	scaffold348: 392952 .. 393902 (-)
RNA-Seq Expression	MS028182
Synteny	MS028182

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCTGGTTCGCGATCGCCGCCACCTGAACCTCCGCCTCCCCGACCTCTCCGACGCCCGCCCCCGCTTCCCTCTACCGCTGCCTCCGTCCTCCGCCGCCGCTCCCGCCGCCTCCCCTGCCATCGCCACCGCCGATCTCGACAAGATCCAAGTCCTCGGCCACGGCAACGGCGGCACAGTCTACAAAGTCCGCCACAAGCGCACTTCCACCACCTACGCCCTCAAGGTCGTCCACGGCGACTGCGATCCAACCGTCCGCCGCCAGGTCTTCAGAGAGATGGAGATTCTCCGGCGTACGGACTCTCCCTACGTGGTCCAGTGCCATGGAATCTTCGAGAAGCCGTCCGGAGACGTGACGATTCTGATGGAGTACATGGATTTAGGATCGCTCGATTCGCTGCTGAAGAAGAACTCGACGTTGCCTGAATCCACGCTCTCTCACGTTTCTCGCCAGGTTCTTAACGGCCTCCACTACCTTCACTCTCACAAAATCATTCATCGCGATATAAAACCGTCGAATCTGTTGGTGAACAAGAATATGGAGGTGAAAATCGCGGATTTCGGAGTCAGTAAAATTATGTGCCGGACTCTGGATGCTTGCAATTCCTACGTCGGAACCTGCGCTTATATGAGTCCGGAGCGGTTCGATCCGGACGCCTACGGCGGAAATTACAACGGTTACGCCGGAGACATTTGGAGTTTGGGGCTCACTCTTCTGGAGCTCTATTTAGGCCATTTTCCTTTTCTGCCGCCCGGTCAGAGGCCGGATTGGGCCACTCTCATGTGCGCGATTTGCTTCGGCGAGCCGCCGACCTTGCCGGAGGACGCGTCGGAGGATTTCCGGAGCTTCGTCGAGTGTTGCTTGCAGAAGGACTCGAGCAAGCGGTGGTCGGCGGCGCAATTGTTGACGCATCCGTTCGTTTGCAAGGAATCGAAACCCGATCGGTGA

mRNA sequence

ATGGCTCTGGTTCGCGATCGCCGCCACCTGAACCTCCGCCTCCCCGACCTCTCCGACGCCCGCCCCCGCTTCCCTCTACCGCTGCCTCCGTCCTCCGCCGCCGCTCCCGCCGCCTCCCCTGCCATCGCCACCGCCGATCTCGACAAGATCCAAGTCCTCGGCCACGGCAACGGCGGCACAGTCTACAAAGTCCGCCACAAGCGCACTTCCACCACCTACGCCCTCAAGGTCGTCCACGGCGACTGCGATCCAACCGTCCGCCGCCAGGTCTTCAGAGAGATGGAGATTCTCCGGCGTACGGACTCTCCCTACGTGGTCCAGTGCCATGGAATCTTCGAGAAGCCGTCCGGAGACGTGACGATTCTGATGGAGTACATGGATTTAGGATCGCTCGATTCGCTGCTGAAGAAGAACTCGACGTTGCCTGAATCCACGCTCTCTCACGTTTCTCGCCAGGTTCTTAACGGCCTCCACTACCTTCACTCTCACAAAATCATTCATCGCGATATAAAACCGTCGAATCTGTTGGTGAACAAGAATATGGAGGTGAAAATCGCGGATTTCGGAGTCAGTAAAATTATGTGCCGGACTCTGGATGCTTGCAATTCCTACGTCGGAACCTGCGCTTATATGAGTCCGGAGCGGTTCGATCCGGACGCCTACGGCGGAAATTACAACGGTTACGCCGGAGACATTTGGAGTTTGGGGCTCACTCTTCTGGAGCTCTATTTAGGCCATTTTCCTTTTCTGCCGCCCGGTCAGAGGCCGGATTGGGCCACTCTCATGTGCGCGATTTGCTTCGGCGAGCCGCCGACCTTGCCGGAGGACGCGTCGGAGGATTTCCGGAGCTTCGTCGAGTGTTGCTTGCAGAAGGACTCGAGCAAGCGGTGGTCGGCGGCGCAATTGTTGACGCATCCGTTCGTTTGCAAGGAATCGAAACCCGATCGGTGA

Coding sequence (CDS)

ATGGCTCTGGTTCGCGATCGCCGCCACCTGAACCTCCGCCTCCCCGACCTCTCCGACGCCCGCCCCCGCTTCCCTCTACCGCTGCCTCCGTCCTCCGCCGCCGCTCCCGCCGCCTCCCCTGCCATCGCCACCGCCGATCTCGACAAGATCCAAGTCCTCGGCCACGGCAACGGCGGCACAGTCTACAAAGTCCGCCACAAGCGCACTTCCACCACCTACGCCCTCAAGGTCGTCCACGGCGACTGCGATCCAACCGTCCGCCGCCAGGTCTTCAGAGAGATGGAGATTCTCCGGCGTACGGACTCTCCCTACGTGGTCCAGTGCCATGGAATCTTCGAGAAGCCGTCCGGAGACGTGACGATTCTGATGGAGTACATGGATTTAGGATCGCTCGATTCGCTGCTGAAGAAGAACTCGACGTTGCCTGAATCCACGCTCTCTCACGTTTCTCGCCAGGTTCTTAACGGCCTCCACTACCTTCACTCTCACAAAATCATTCATCGCGATATAAAACCGTCGAATCTGTTGGTGAACAAGAATATGGAGGTGAAAATCGCGGATTTCGGAGTCAGTAAAATTATGTGCCGGACTCTGGATGCTTGCAATTCCTACGTCGGAACCTGCGCTTATATGAGTCCGGAGCGGTTCGATCCGGACGCCTACGGCGGAAATTACAACGGTTACGCCGGAGACATTTGGAGTTTGGGGCTCACTCTTCTGGAGCTCTATTTAGGCCATTTTCCTTTTCTGCCGCCCGGTCAGAGGCCGGATTGGGCCACTCTCATGTGCGCGATTTGCTTCGGCGAGCCGCCGACCTTGCCGGAGGACGCGTCGGAGGATTTCCGGAGCTTCGTCGAGTGTTGCTTGCAGAAGGACTCGAGCAAGCGGTGGTCGGCGGCGCAATTGTTGACGCATCCGTTCGTTTGCAAGGAATCGAAACCCGATCGGTGA

Protein sequence

MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSAAAPAASPAIATADLDKIQVLGHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRWSAAQLLTHPFVCKESKPDR

Homology

[Close]

BLAST of MS028182 vs. NCBI nr
Match: XP_023001803.1 (mitogen-activated protein kinase kinase 9-like [Cucurbita maxima])

HSP 1 Score: 618.2 bits (1593), Expect = 3.8e-173
Identity = 297/319 (93.10%), Postives = 306/319 (95.92%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSAAAPAASPAI---ATADLDKIQVLGHGN 60
           MALVRDRR LNLRLPDLSD RPRFPLPLPPSSAAAPAA+ A    A +DL+K+QVLGHGN
Sbjct: 1   MALVRDRRQLNLRLPDLSDCRPRFPLPLPPSSAAAPAATAAAAAPAASDLEKLQVLGHGN 60

Query: 61  GGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG 120
           GGTVYKVRHKRTS TYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG
Sbjct: 61  GGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG 120

Query: 121 DVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLV 180
           DVTILMEYMDLGSLDSLLKKNSTL ESTL+HV+RQVLNGLHYLHSHKIIHRDIKPSNLLV
Sbjct: 121 DVTILMEYMDLGSLDSLLKKNSTLSESTLAHVARQVLNGLHYLHSHKIIHRDIKPSNLLV 180

Query: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGL 240
           NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPD YGGNYNGYAGDIWSLGL
Sbjct: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDTYGGNYNGYAGDIWSLGL 240

Query: 241 TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRW 300
           TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQK+SSKRW
Sbjct: 241 TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKESSKRW 300

Query: 301 SAAQLLTHPFVCKESKPDR 317
           + AQLLTHPFVCKES+ DR
Sbjct: 301 TVAQLLTHPFVCKESRSDR 319

[Close]

BLAST of MS028182 vs. NCBI nr
Match: XP_022924181.1 (mitogen-activated protein kinase kinase 9-like [Cucurbita moschata] >XP_023518975.1 mitogen-activated protein kinase kinase 9-like [Cucurbita pepo subsp. pepo] >KAG6584304.1 Mitogen-activated protein kinase kinase 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 617.1 bits (1590), Expect = 8.6e-173
Identity = 296/319 (92.79%), Postives = 306/319 (95.92%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSAAAPAASPAI---ATADLDKIQVLGHGN 60
           MALVRDRR LNLRLPDLSD RPRFPLPLPPSSAAAPAA+ A    A +DL+K+QVLGHGN
Sbjct: 1   MALVRDRRQLNLRLPDLSDCRPRFPLPLPPSSAAAPAATAAAAAPAASDLEKLQVLGHGN 60

Query: 61  GGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG 120
           GGTVYKVRHKRTS TYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG
Sbjct: 61  GGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG 120

Query: 121 DVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLV 180
           DVTILMEYMDLGSLDSLLKKNSTL E+TL+HV+RQVLNGLHYLHSHKIIHRDIKPSNLLV
Sbjct: 121 DVTILMEYMDLGSLDSLLKKNSTLSEATLAHVARQVLNGLHYLHSHKIIHRDIKPSNLLV 180

Query: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGL 240
           NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPD YGGNYNGYAGDIWSLGL
Sbjct: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDTYGGNYNGYAGDIWSLGL 240

Query: 241 TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRW 300
           TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQK+SSKRW
Sbjct: 241 TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKESSKRW 300

Query: 301 SAAQLLTHPFVCKESKPDR 317
           + AQLLTHPFVCKES+ DR
Sbjct: 301 TVAQLLTHPFVCKESRSDR 319

[Close]

BLAST of MS028182 vs. NCBI nr
Match: XP_004137516.1 (mitogen-activated protein kinase kinase 9 [Cucumis sativus] >KGN64193.1 hypothetical protein Csa_014003 [Cucumis sativus])

HSP 1 Score: 611.3 bits (1575), Expect = 4.7e-171
Identity = 293/313 (93.61%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSA-AAPAASPAIATADLDKIQVLGHGNGG 60
           MALVRDRRHLNLRLPDLSD RPRFPLPLPPSSA  APAA  AI+++DLDK+QVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPPAPAAPSAISSSDLDKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120
           TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMDLGSLDSLLKKNSTL E+TL+HVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDP+ YGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRWSA 300
           LELYLGHFPFLP GQRPDWATLMCAICFGEPP LPEDASE+FRSFVECCLQK+SSKRW+A
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVCKES 313
           AQLLTHPFVC+ES
Sbjct: 301 AQLLTHPFVCRES 313

[Close]

BLAST of MS028182 vs. NCBI nr
Match: XP_008466859.1 (PREDICTED: mitogen-activated protein kinase kinase 9 [Cucumis melo] >TYJ99050.1 mitogen-activated protein kinase kinase 9 [Cucumis melo var. makuwa])

HSP 1 Score: 609.8 bits (1571), Expect = 1.4e-170
Identity = 292/313 (93.29%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSA-AAPAASPAIATADLDKIQVLGHGNGG 60
           MALVRDRRHLNLRLPDLSD RPRFPLPLPPSSA  APAA  AI+++DL+K+QVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPPAPAAPSAISSSDLEKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120
           TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMDLGSLDSLLKKNSTL E+TL+HVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSETTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDP+ YGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRWSA 300
           LELYLGHFPFLP GQRPDWATLMCAICFGEPP LPEDASE+FRSFVECCLQK+SSKRW+A
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVCKES 313
           AQLLTHPFVC+ES
Sbjct: 301 AQLLTHPFVCRES 313

[Close]

BLAST of MS028182 vs. NCBI nr
Match: CUX76643.1 (MAP kinase Kinase 4 [Cucumis sativus])

HSP 1 Score: 606.3 bits (1562), Expect = 1.5e-169
Identity = 291/313 (92.97%), Postives = 302/313 (96.49%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSA-AAPAASPAIATADLDKIQVLGHGNGG 60
           MALVRDRRHLNLRLPDLSD RPRF LPLPPSSA  APAA  AI+++DLDK+QVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFSLPLPPSSAPPAPAAPSAISSSDLDKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120
           TVYKVRHKRTS TYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMDLGSLDSLLKKNSTL E+TL+HVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDP+ YGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRWSA 300
           LELYLGHFPFLP GQRPDWATLMCAICFGEPP LPEDASE+FRSFVECCLQK+SSKRW+A
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVCKES 313
           AQLLTHPFVC+ES
Sbjct: 301 AQLLTHPFVCRES 313

[Close]

BLAST of MS028182 vs. ExPASy Swiss-Prot
Match: Q9FX43 (Mitogen-activated protein kinase kinase 9 OS=Arabidopsis thaliana OX=3702 GN=MKK9 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 3.6e-113
Identity = 202/309 (65.37%), Postives = 239/309 (77.35%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSAAAPAASPAIATADLDKIQVLGHGNGGT 60
           MALVR+RR LNLRLP    +  RF      ++    A    I+  DL+K+ VLG GNGG 
Sbjct: 1   MALVRERRQLNLRLPLPPISDRRFSTSSSSATTTTVAGCNGISACDLEKLNVLGCGNGGI 60

Query: 61  VYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKP-SGDV 120
           VYKVRHK TS  YALK V+GD DP   RQ+ REMEILRRTDSPYVV+CHGIFEKP  G+V
Sbjct: 61  VYKVRHKTTSEIYALKTVNGDMDPIFTRQLMREMEILRRTDSPYVVKCHGIFEKPVVGEV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           +ILMEYMD G+L+SL      + E  L+  ++Q+L GL YLH+ KI+HRDIKP+NLL+N 
Sbjct: 121 SILMEYMDGGTLESL---RGGVTEQKLAGFAKQILKGLSYLHALKIVHRDIKPANLLLNS 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGLTL 240
             EVKIADFGVSKI+ R+LD+CNSYVGTCAYMSPERFD ++ GG+ + YAGDIWS GL +
Sbjct: 181 KNEVKIADFGVSKILVRSLDSCNSYVGTCAYMSPERFDSESSGGSSDIYAGDIWSFGLMM 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRWSA 300
           LEL +GHFP LPPGQRPDWATLMCA+CFGEPP  PE  SE+FRSFVECCL+KDSSKRW+A
Sbjct: 241 LELLVGHFPLLPPGQRPDWATLMCAVCFGEPPRAPEGCSEEFRSFVECCLRKDSSKRWTA 300

Query: 301 AQLLTHPFV 309
            QLL HPF+
Sbjct: 301 PQLLAHPFL 306

[Close]

BLAST of MS028182 vs. ExPASy Swiss-Prot
Match: Q9LPQ3 (Mitogen-activated protein kinase kinase 7 OS=Arabidopsis thaliana OX=3702 GN=MKK7 PE=1 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 7.2e-106
Identity = 195/311 (62.70%), Postives = 235/311 (75.56%), Query Frame = 0


Query: 1   MALVRDRRHLNLRL--PDLSDARPRFPLPLPPSSAAAPAASPAIATADLDKIQVLGHGNG 60
           MALVR RR +NLRL  P LS   P F      +S+ AP  +  I+ +D++K+ VLG G+ 
Sbjct: 1   MALVRKRRQINLRLPVPPLSVHLPWFSF----ASSTAPVINNGISASDVEKLHVLGRGSS 60

Query: 61  GTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKP-SG 120
           G VYKV HK T   YALK V+GD  P   RQ+ REMEILRRTDSPYVV+C GIFEKP  G
Sbjct: 61  GIVYKVHHKTTGEIYALKSVNGDMSPAFTRQLAREMEILRRTDSPYVVRCQGIFEKPIVG 120

Query: 121 DVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLV 180
           +V+ILMEYMD G+L+SL      + E  L+  SRQ+L GL YLHS KI+HRDIKP+NLL+
Sbjct: 121 EVSILMEYMDGGNLESL---RGAVTEKQLAGFSRQILKGLSYLHSLKIVHRDIKPANLLL 180

Query: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGL 240
           N   EVKIADFGVSKI+ R+LD CNSYVGTCAYMSPERFD  A G N + YAGDIWS G+
Sbjct: 181 NSRNEVKIADFGVSKIITRSLDYCNSYVGTCAYMSPERFD-SAAGENSDVYAGDIWSFGV 240

Query: 241 TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRW 300
            +LEL++GHFP LP GQRPDWATLMC +CFGEPP  PE  S++FRSFV+CCL+K+SS+RW
Sbjct: 241 MILELFVGHFPLLPQGQRPDWATLMCVVCFGEPPRAPEGCSDEFRSFVDCCLRKESSERW 300

Query: 301 SAAQLLTHPFV 309
           +A+QLL HPF+
Sbjct: 301 TASQLLGHPFL 303

[Close]

BLAST of MS028182 vs. ExPASy Swiss-Prot
Match: Q8RXG3 (Mitogen-activated protein kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MKK5 PE=1 SV=2)

HSP 1 Score: 328.6 bits (841), Expect = 8.0e-89
Identity = 169/317 (53.31%), Postives = 221/317 (69.72%), Query Frame = 0


Query: 4   VRDRRHLNLRLPDLSDARPRFPLPLPP--SSAAAPAASPAIAT--------ADLDKIQVL 63
           +R R  L+L LP   D     PLPLPP  SS++APA+S AI+T        ++L+++  +
Sbjct: 18  LRKRPDLSLPLPH-RDVALAVPLPLPPPSSSSSAPASSSAISTNISAAKSLSELERVNRI 77

Query: 64  GHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFE 123
           G G GGTVYKV H  TS  +ALKV++G+ + TVRRQ+ RE+EILR  D P VV+CH +F+
Sbjct: 78  GSGAGGTVYKVIHTPTSRPFALKVIYGNHEDTVRRQICREIEILRSVDHPNVVKCHDMFD 137

Query: 124 KPSGDVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPS 183
             +G++ +L+E+MD GSL+          E  L+ +SRQ+L+GL YLH   I+HRDIKPS
Sbjct: 138 H-NGEIQVLLEFMDQGSLEGA----HIWQEQELADLSRQILSGLAYLHRRHIVHRDIKPS 197

Query: 184 NLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIW 243
           NLL+N    VKIADFGVS+I+ +T+D CNS VGT AYMSPER + D   G Y+GYAGD+W
Sbjct: 198 NLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNHGRYDGYAGDVW 257

Query: 244 SLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDS 303
           SLG+++LE YLG FPF    ++ DWA+LMCAIC  +PP  P  AS++FR FV CCLQ D 
Sbjct: 258 SLGVSILEFYLGRFPF-AVSRQGDWASLMCAICMSQPPEAPATASQEFRHFVSCCLQSDP 317

Query: 304 SKRWSAAQLLTHPFVCK 311
            KRWSA QLL HPF+ K
Sbjct: 318 PKRWSAQQLLQHPFILK 327

[Close]

BLAST of MS028182 vs. ExPASy Swiss-Prot
Match: Q9M8J5 (Mitogen-activated protein kinase kinase 8 OS=Arabidopsis thaliana OX=3702 GN=MKK8 PE=3 SV=2)

HSP 1 Score: 321.2 bits (822), Expect = 1.3e-86
Identity = 169/318 (53.14%), Postives = 221/318 (69.50%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARP----RFPL--PLPPSSAAAPAASPAIATADLDKIQVLG 60
           M +VRD + LNL+L  +         RFP+      S+  +  AS   + A+LD+I VLG
Sbjct: 1   MVMVRDNQFLNLKLSPIQAPTTIPPCRFPIIPATKVSATVSSCASNTFSVANLDRISVLG 60

Query: 61  HGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEK 120
            GNGGTV+KV+ K TS  YALK V  + D T      RE+EILR  +SPYV +CH IF+ 
Sbjct: 61  SGNGGTVFKVKDKTTSEIYALKKVKENWDST----SLREIEILRMVNSPYVAKCHDIFQN 120

Query: 121 PSGDVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSN 180
           PSG+V+ILM+YMDLGSL+SL      + E  L+ +SRQVL G +YLH HKI+HRDIKP+N
Sbjct: 121 PSGEVSILMDYMDLGSLESL----RGVTEKQLALMSRQVLEGKNYLHEHKIVHRDIKPAN 180

Query: 181 LLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYG----GNYNGYAG 240
           LL +   EVKIADFGVSKI+ R+L+ CNS+VGT AYMSPER D +A G       N YAG
Sbjct: 181 LLRSSKEEVKIADFGVSKIVVRSLNKCNSFVGTFAYMSPERLDSEADGVTEEDKSNVYAG 240

Query: 241 DIWSLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQ 300
           DIWS GLT+LE+ +G++P L     PD A ++CA+CFGEPP  PE+ S+D +SF++CCL+
Sbjct: 241 DIWSFGLTMLEILVGYYPML-----PDQAAIVCAVCFGEPPKAPEECSDDLKSFMDCCLR 300

Query: 301 KDSSKRWSAAQLLTHPFV 309
           K +S+RW+A+QLL HPF+
Sbjct: 301 KKASERWTASQLLNHPFL 305

[Close]

BLAST of MS028182 vs. ExPASy Swiss-Prot
Match: O80397 (Mitogen-activated protein kinase kinase 4 OS=Arabidopsis thaliana OX=3702 GN=MKK4 PE=1 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.3e-83
Identity = 164/328 (50.00%), Postives = 219/328 (66.77%), Query Frame = 0


Query: 5   RDRRHLNLRLP-DLSDARPRFPLPLPP-------SSAAAPAASPAIAT------------ 64
           R RR  +L LP    D     PLPLPP       SS +AP++  + ++            
Sbjct: 17  RPRRRPDLTLPLPQRDVSLAVPLPLPPTSGGSGGSSGSAPSSGGSASSTNTNSSIEAKNY 76

Query: 65  ADLDKIQVLGHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPY 124
           +DL +   +G G GGTVYKV H+ +S  YALKV++G+ + TVRRQ+ RE+EILR  + P 
Sbjct: 77  SDLVRGNRIGSGAGGTVYKVIHRPSSRLYALKVIYGNHEETVRRQICREIEILRDVNHPN 136

Query: 125 VVQCHGIFEKPSGDVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHK 184
           VV+CH +F++ +G++ +L+E+MD GSL+          E  L+ +SRQ+L+GL YLHS  
Sbjct: 137 VVKCHEMFDQ-NGEIQVLLEFMDKGSLEGA----HVWKEQQLADLSRQILSGLAYLHSRH 196

Query: 185 IIHRDIKPSNLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGN 244
           I+HRDIKPSNLL+N    VKIADFGVS+I+ +T+D CNS VGT AYMSPER + D   G 
Sbjct: 197 IVHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNQGK 256

Query: 245 YNGYAGDIWSLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSF 304
           Y+GYAGDIWSLG+++LE YLG FPF P  ++ DWA+LMCAIC  +PP  P  AS +FR F
Sbjct: 257 YDGYAGDIWSLGVSILEFYLGRFPF-PVSRQGDWASLMCAICMSQPPEAPATASPEFRHF 316

Query: 305 VECCLQKDSSKRWSAAQLLTHPFVCKES 313
           + CCLQ++  KR SA QLL HPF+ + S
Sbjct: 317 ISCCLQREPGKRRSAMQLLQHPFILRAS 338

[Close]

BLAST of MS028182 vs. ExPASy TrEMBL
Match: A0A6J1KRM1 (mitogen-activated protein kinase kinase 9-like OS=Cucurbita maxima OX=3661 GN=LOC111495833 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 1.9e-173
Identity = 297/319 (93.10%), Postives = 306/319 (95.92%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSAAAPAASPAI---ATADLDKIQVLGHGN 60
           MALVRDRR LNLRLPDLSD RPRFPLPLPPSSAAAPAA+ A    A +DL+K+QVLGHGN
Sbjct: 1   MALVRDRRQLNLRLPDLSDCRPRFPLPLPPSSAAAPAATAAAAAPAASDLEKLQVLGHGN 60

Query: 61  GGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG 120
           GGTVYKVRHKRTS TYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG
Sbjct: 61  GGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG 120

Query: 121 DVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLV 180
           DVTILMEYMDLGSLDSLLKKNSTL ESTL+HV+RQVLNGLHYLHSHKIIHRDIKPSNLLV
Sbjct: 121 DVTILMEYMDLGSLDSLLKKNSTLSESTLAHVARQVLNGLHYLHSHKIIHRDIKPSNLLV 180

Query: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGL 240
           NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPD YGGNYNGYAGDIWSLGL
Sbjct: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDTYGGNYNGYAGDIWSLGL 240

Query: 241 TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRW 300
           TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQK+SSKRW
Sbjct: 241 TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKESSKRW 300

Query: 301 SAAQLLTHPFVCKESKPDR 317
           + AQLLTHPFVCKES+ DR
Sbjct: 301 TVAQLLTHPFVCKESRSDR 319

[Close]

BLAST of MS028182 vs. ExPASy TrEMBL
Match: A0A6J1E8V9 (mitogen-activated protein kinase kinase 9-like OS=Cucurbita moschata OX=3662 GN=LOC111431703 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 4.2e-173
Identity = 296/319 (92.79%), Postives = 306/319 (95.92%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSAAAPAASPAI---ATADLDKIQVLGHGN 60
           MALVRDRR LNLRLPDLSD RPRFPLPLPPSSAAAPAA+ A    A +DL+K+QVLGHGN
Sbjct: 1   MALVRDRRQLNLRLPDLSDCRPRFPLPLPPSSAAAPAATAAAAAPAASDLEKLQVLGHGN 60

Query: 61  GGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG 120
           GGTVYKVRHKRTS TYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG
Sbjct: 61  GGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG 120

Query: 121 DVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLV 180
           DVTILMEYMDLGSLDSLLKKNSTL E+TL+HV+RQVLNGLHYLHSHKIIHRDIKPSNLLV
Sbjct: 121 DVTILMEYMDLGSLDSLLKKNSTLSEATLAHVARQVLNGLHYLHSHKIIHRDIKPSNLLV 180

Query: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGL 240
           NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPD YGGNYNGYAGDIWSLGL
Sbjct: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDTYGGNYNGYAGDIWSLGL 240

Query: 241 TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRW 300
           TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQK+SSKRW
Sbjct: 241 TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKESSKRW 300

Query: 301 SAAQLLTHPFVCKESKPDR 317
           + AQLLTHPFVCKES+ DR
Sbjct: 301 TVAQLLTHPFVCKESRSDR 319

[Close]

BLAST of MS028182 vs. ExPASy TrEMBL
Match: A0A0A0LQY3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042980 PE=3 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.3e-171
Identity = 293/313 (93.61%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSA-AAPAASPAIATADLDKIQVLGHGNGG 60
           MALVRDRRHLNLRLPDLSD RPRFPLPLPPSSA  APAA  AI+++DLDK+QVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPPAPAAPSAISSSDLDKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120
           TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMDLGSLDSLLKKNSTL E+TL+HVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDP+ YGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRWSA 300
           LELYLGHFPFLP GQRPDWATLMCAICFGEPP LPEDASE+FRSFVECCLQK+SSKRW+A
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVCKES 313
           AQLLTHPFVC+ES
Sbjct: 301 AQLLTHPFVCRES 313

[Close]

BLAST of MS028182 vs. ExPASy TrEMBL
Match: A0A5D3BGX9 (Mitogen-activated protein kinase kinase 9 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002540 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 6.6e-171
Identity = 292/313 (93.29%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSA-AAPAASPAIATADLDKIQVLGHGNGG 60
           MALVRDRRHLNLRLPDLSD RPRFPLPLPPSSA  APAA  AI+++DL+K+QVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPPAPAAPSAISSSDLEKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120
           TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMDLGSLDSLLKKNSTL E+TL+HVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSETTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDP+ YGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRWSA 300
           LELYLGHFPFLP GQRPDWATLMCAICFGEPP LPEDASE+FRSFVECCLQK+SSKRW+A
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVCKES 313
           AQLLTHPFVC+ES
Sbjct: 301 AQLLTHPFVCRES 313

[Close]

BLAST of MS028182 vs. ExPASy TrEMBL
Match: A0A1S3CS75 (mitogen-activated protein kinase kinase 9 OS=Cucumis melo OX=3656 GN=LOC103504136 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 6.6e-171
Identity = 292/313 (93.29%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSA-AAPAASPAIATADLDKIQVLGHGNGG 60
           MALVRDRRHLNLRLPDLSD RPRFPLPLPPSSA  APAA  AI+++DL+K+QVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPPAPAAPSAISSSDLEKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120
           TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMDLGSLDSLLKKNSTL E+TL+HVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSETTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDP+ YGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRWSA 300
           LELYLGHFPFLP GQRPDWATLMCAICFGEPP LPEDASE+FRSFVECCLQK+SSKRW+A
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVCKES 313
           AQLLTHPFVC+ES
Sbjct: 301 AQLLTHPFVCRES 313

[Close]

BLAST of MS028182 vs. TAIR 10
Match: AT1G73500.1 (MAP kinase kinase 9 )

HSP 1 Score: 409.5 bits (1051), Expect = 2.5e-114
Identity = 202/309 (65.37%), Postives = 239/309 (77.35%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARPRFPLPLPPSSAAAPAASPAIATADLDKIQVLGHGNGGT 60
           MALVR+RR LNLRLP    +  RF      ++    A    I+  DL+K+ VLG GNGG 
Sbjct: 1   MALVRERRQLNLRLPLPPISDRRFSTSSSSATTTTVAGCNGISACDLEKLNVLGCGNGGI 60

Query: 61  VYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKP-SGDV 120
           VYKVRHK TS  YALK V+GD DP   RQ+ REMEILRRTDSPYVV+CHGIFEKP  G+V
Sbjct: 61  VYKVRHKTTSEIYALKTVNGDMDPIFTRQLMREMEILRRTDSPYVVKCHGIFEKPVVGEV 120

Query: 121 TILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           +ILMEYMD G+L+SL      + E  L+  ++Q+L GL YLH+ KI+HRDIKP+NLL+N 
Sbjct: 121 SILMEYMDGGTLESL---RGGVTEQKLAGFAKQILKGLSYLHALKIVHRDIKPANLLLNS 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGLTL 240
             EVKIADFGVSKI+ R+LD+CNSYVGTCAYMSPERFD ++ GG+ + YAGDIWS GL +
Sbjct: 181 KNEVKIADFGVSKILVRSLDSCNSYVGTCAYMSPERFDSESSGGSSDIYAGDIWSFGLMM 240

Query: 241 LELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRWSA 300
           LEL +GHFP LPPGQRPDWATLMCA+CFGEPP  PE  SE+FRSFVECCL+KDSSKRW+A
Sbjct: 241 LELLVGHFPLLPPGQRPDWATLMCAVCFGEPPRAPEGCSEEFRSFVECCLRKDSSKRWTA 300

Query: 301 AQLLTHPFV 309
            QLL HPF+
Sbjct: 301 PQLLAHPFL 306

[Close]

BLAST of MS028182 vs. TAIR 10
Match: AT1G18350.1 (MAP kinase kinase 7 )

HSP 1 Score: 385.2 bits (988), Expect = 5.1e-107
Identity = 195/311 (62.70%), Postives = 235/311 (75.56%), Query Frame = 0


Query: 1   MALVRDRRHLNLRL--PDLSDARPRFPLPLPPSSAAAPAASPAIATADLDKIQVLGHGNG 60
           MALVR RR +NLRL  P LS   P F      +S+ AP  +  I+ +D++K+ VLG G+ 
Sbjct: 1   MALVRKRRQINLRLPVPPLSVHLPWFSF----ASSTAPVINNGISASDVEKLHVLGRGSS 60

Query: 61  GTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKP-SG 120
           G VYKV HK T   YALK V+GD  P   RQ+ REMEILRRTDSPYVV+C GIFEKP  G
Sbjct: 61  GIVYKVHHKTTGEIYALKSVNGDMSPAFTRQLAREMEILRRTDSPYVVRCQGIFEKPIVG 120

Query: 121 DVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSNLLV 180
           +V+ILMEYMD G+L+SL      + E  L+  SRQ+L GL YLHS KI+HRDIKP+NLL+
Sbjct: 121 EVSILMEYMDGGNLESL---RGAVTEKQLAGFSRQILKGLSYLHSLKIVHRDIKPANLLL 180

Query: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIWSLGL 240
           N   EVKIADFGVSKI+ R+LD CNSYVGTCAYMSPERFD  A G N + YAGDIWS G+
Sbjct: 181 NSRNEVKIADFGVSKIITRSLDYCNSYVGTCAYMSPERFD-SAAGENSDVYAGDIWSFGV 240

Query: 241 TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDSSKRW 300
            +LEL++GHFP LP GQRPDWATLMC +CFGEPP  PE  S++FRSFV+CCL+K+SS+RW
Sbjct: 241 MILELFVGHFPLLPQGQRPDWATLMCVVCFGEPPRAPEGCSDEFRSFVDCCLRKESSERW 300

Query: 301 SAAQLLTHPFV 309
           +A+QLL HPF+
Sbjct: 301 TASQLLGHPFL 303

[Close]

BLAST of MS028182 vs. TAIR 10
Match: AT3G21220.1 (MAP kinase kinase 5 )

HSP 1 Score: 328.6 bits (841), Expect = 5.7e-90
Identity = 169/317 (53.31%), Postives = 221/317 (69.72%), Query Frame = 0


Query: 4   VRDRRHLNLRLPDLSDARPRFPLPLPP--SSAAAPAASPAIAT--------ADLDKIQVL 63
           +R R  L+L LP   D     PLPLPP  SS++APA+S AI+T        ++L+++  +
Sbjct: 18  LRKRPDLSLPLPH-RDVALAVPLPLPPPSSSSSAPASSSAISTNISAAKSLSELERVNRI 77

Query: 64  GHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFE 123
           G G GGTVYKV H  TS  +ALKV++G+ + TVRRQ+ RE+EILR  D P VV+CH +F+
Sbjct: 78  GSGAGGTVYKVIHTPTSRPFALKVIYGNHEDTVRRQICREIEILRSVDHPNVVKCHDMFD 137

Query: 124 KPSGDVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPS 183
             +G++ +L+E+MD GSL+          E  L+ +SRQ+L+GL YLH   I+HRDIKPS
Sbjct: 138 H-NGEIQVLLEFMDQGSLEGA----HIWQEQELADLSRQILSGLAYLHRRHIVHRDIKPS 197

Query: 184 NLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGNYNGYAGDIW 243
           NLL+N    VKIADFGVS+I+ +T+D CNS VGT AYMSPER + D   G Y+GYAGD+W
Sbjct: 198 NLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNHGRYDGYAGDVW 257

Query: 244 SLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKDS 303
           SLG+++LE YLG FPF    ++ DWA+LMCAIC  +PP  P  AS++FR FV CCLQ D 
Sbjct: 258 SLGVSILEFYLGRFPF-AVSRQGDWASLMCAICMSQPPEAPATASQEFRHFVSCCLQSDP 317

Query: 304 SKRWSAAQLLTHPFVCK 311
            KRWSA QLL HPF+ K
Sbjct: 318 PKRWSAQQLLQHPFILK 327

[Close]

BLAST of MS028182 vs. TAIR 10
Match: AT1G51660.1 (mitogen-activated protein kinase kinase 4 )

HSP 1 Score: 310.5 bits (794), Expect = 1.6e-84
Identity = 164/328 (50.00%), Postives = 219/328 (66.77%), Query Frame = 0


Query: 5   RDRRHLNLRLP-DLSDARPRFPLPLPP-------SSAAAPAASPAIAT------------ 64
           R RR  +L LP    D     PLPLPP       SS +AP++  + ++            
Sbjct: 17  RPRRRPDLTLPLPQRDVSLAVPLPLPPTSGGSGGSSGSAPSSGGSASSTNTNSSIEAKNY 76

Query: 65  ADLDKIQVLGHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPY 124
           +DL +   +G G GGTVYKV H+ +S  YALKV++G+ + TVRRQ+ RE+EILR  + P 
Sbjct: 77  SDLVRGNRIGSGAGGTVYKVIHRPSSRLYALKVIYGNHEETVRRQICREIEILRDVNHPN 136

Query: 125 VVQCHGIFEKPSGDVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHK 184
           VV+CH +F++ +G++ +L+E+MD GSL+          E  L+ +SRQ+L+GL YLHS  
Sbjct: 137 VVKCHEMFDQ-NGEIQVLLEFMDKGSLEGA----HVWKEQQLADLSRQILSGLAYLHSRH 196

Query: 185 IIHRDIKPSNLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYGGN 244
           I+HRDIKPSNLL+N    VKIADFGVS+I+ +T+D CNS VGT AYMSPER + D   G 
Sbjct: 197 IVHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNQGK 256

Query: 245 YNGYAGDIWSLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSF 304
           Y+GYAGDIWSLG+++LE YLG FPF P  ++ DWA+LMCAIC  +PP  P  AS +FR F
Sbjct: 257 YDGYAGDIWSLGVSILEFYLGRFPF-PVSRQGDWASLMCAICMSQPPEAPATASPEFRHF 316

Query: 305 VECCLQKDSSKRWSAAQLLTHPFVCKES 313
           + CCLQ++  KR SA QLL HPF+ + S
Sbjct: 317 ISCCLQREPGKRRSAMQLLQHPFILRAS 338

[Close]

BLAST of MS028182 vs. TAIR 10
Match: AT3G06230.1 (MAP kinase kinase 8 )

HSP 1 Score: 300.4 bits (768), Expect = 1.7e-81
Identity = 161/306 (52.61%), Postives = 210/306 (68.63%), Query Frame = 0


Query: 1   MALVRDRRHLNLRLPDLSDARP----RFPL--PLPPSSAAAPAASPAIATADLDKIQVLG 60
           M +VRD + LNL+L  +         RFP+      S+  +  AS   + A+LD+I VLG
Sbjct: 1   MVMVRDNQFLNLKLSPIQAPTTIPPCRFPIIPATKVSATVSSCASNTFSVANLDRISVLG 60

Query: 61  HGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEK 120
            GNGGTV+KV+ K TS  YALK V  + D T      RE+EILR  +SPYV +CH IF+ 
Sbjct: 61  SGNGGTVFKVKDKTTSEIYALKKVKENWDST----SLREIEILRMVNSPYVAKCHDIFQN 120

Query: 121 PSGDVTILMEYMDLGSLDSLLKKNSTLPESTLSHVSRQVLNGLHYLHSHKIIHRDIKPSN 180
           PSG+V+ILM+YMDLGSL+SL      + E  L+ +SRQVL G +YLH HKI+HRDIKP+N
Sbjct: 121 PSGEVSILMDYMDLGSLESL----RGVTEKQLALMSRQVLEGKNYLHEHKIVHRDIKPAN 180

Query: 181 LLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDAYG----GNYNGYAG 240
           LL +   EVKIADFGVSKI+ R+L+ CNS+VGT AYMSPER D +A G       N YAG
Sbjct: 181 LLRSSKEEVKIADFGVSKIVVRSLNKCNSFVGTFAYMSPERLDSEADGVTEEDKSNVYAG 240

Query: 241 DIWSLGLTLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQ 297
           DIWS GLT+LE+ +G++P L     PD A ++CA+CFGEPP  PE+ S+D +SF++CCL+
Sbjct: 241 DIWSFGLTMLEILVGYYPML-----PDQAAIVCAVCFGEPPKAPEECSDDLKSFMDCCLR 293

The following BLAST results are available for this feature:

BLAST of MS028182 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023001803.1	3.8e-173	93.10	mitogen-activated protein kinase kinase 9-like [Cucurbita maxima]	[more]
XP_022924181.1	8.6e-173	92.79	mitogen-activated protein kinase kinase 9-like [Cucurbita moschata] >XP_02351897...	[more]
XP_004137516.1	4.7e-171	93.61	mitogen-activated protein kinase kinase 9 [Cucumis sativus] >KGN64193.1 hypothet...	[more]
XP_008466859.1	1.4e-170	93.29	PREDICTED: mitogen-activated protein kinase kinase 9 [Cucumis melo] >TYJ99050.1 ...	[more]
CUX76643.1	1.5e-169	92.97	MAP kinase Kinase 4 [Cucumis sativus]	[more]

BLAST of MS028182 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FX43	3.6e-113	65.37	Mitogen-activated protein kinase kinase 9 OS=Arabidopsis thaliana OX=3702 GN=MKK...	[more]
Q9LPQ3	7.2e-106	62.70	Mitogen-activated protein kinase kinase 7 OS=Arabidopsis thaliana OX=3702 GN=MKK...	[more]
Q8RXG3	8.0e-89	53.31	Mitogen-activated protein kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MKK...	[more]
Q9M8J5	1.3e-86	53.14	Mitogen-activated protein kinase kinase 8 OS=Arabidopsis thaliana OX=3702 GN=MKK...	[more]
O80397	2.3e-83	50.00	Mitogen-activated protein kinase kinase 4 OS=Arabidopsis thaliana OX=3702 GN=MKK...	[more]

BLAST of MS028182 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KRM1	1.9e-173	93.10	mitogen-activated protein kinase kinase 9-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1E8V9	4.2e-173	92.79	mitogen-activated protein kinase kinase 9-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A0A0LQY3	2.3e-171	93.61	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042...	[more]
A0A5D3BGX9	6.6e-171	93.29	Mitogen-activated protein kinase kinase 9 OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3CS75	6.6e-171	93.29	mitogen-activated protein kinase kinase 9 OS=Cucumis melo OX=3656 GN=LOC10350413...	[more]

BLAST of MS028182 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73500.1	2.5e-114	65.37	MAP kinase kinase 9	[more]
AT1G18350.1	5.1e-107	62.70	MAP kinase kinase 7	[more]
AT3G21220.1	5.7e-90	53.31	MAP kinase kinase 5	[more]
AT1G51660.1	1.6e-84	50.00	mitogen-activated protein kinase kinase 4	[more]
AT3G06230.1	1.7e-81	52.61	MAP kinase kinase 8	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 47..308 e-value: 2.2E-91 score: 319.6
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 49..308 e-value: 2.2E-65 score: 220.6
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 47..308 score: 46.505821
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 129..315 e-value: 3.0E-55 score: 189.4
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 32..127 e-value: 1.9E-29 score: 103.8
None	No IPR available	PIRSR	PIRSR037568-2	PIRSR037568-2	coord: 44..250 e-value: 7.5E-32 score: 107.6
None	No IPR available	PIRSR	PIRSR000606-51	PIRSR000606-51	coord: 45..300 e-value: 1.3E-35 score: 120.7
None	No IPR available	PIRSR	PIRSR630220-2	PIRSR630220-2	coord: 23..245 e-value: 2.6E-16 score: 56.8
None	No IPR available	PANTHER	PTHR24361:SF769	SERINE/THREONINE-PROTEIN KINASE C01C4.3-RELATED	coord: 5..312
None	No IPR available	PANTHER	PTHR24361	MITOGEN-ACTIVATED KINASE KINASE KINASE	coord: 5..312
None	No IPR available	CDD	cd06623	PKc_MAPKK_plant_like	coord: 45..308 e-value: 2.02693E-144 score: 405.822
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 53..76
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 165..177
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 44..311

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS028182.1	MS028182.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity