MS027905 (gene) Bitter gourd (TR) v1

Overview
NameMS027905
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiondisease resistance protein RGA2-like
Locationscaffold375: 93484 .. 98824 (-)
RNA-Seq ExpressionMS027905
SyntenyMS027905
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCCTAAGGGATGGAGCGACTAGGGCCTTGGGTACAACCTACCTTGAGTTCTCAAGTTTGAGCCGTTCAAGCAACGACAGTCACATGACATCGATTCCAAAAAATAAGAAAAATGTATGGATACTAAATTTAAATAGACATCATGACATTATTGTAGAGATAGAAATGATTATTTTTTTCTTGGTCCAAAATAGGATTTACTCATATTTAAAAATTAACAATATATTTGTTATACTTTTCATATTGTTCTTTTCATGGTTTTATTCTAGTTGCCAGCTGTTGATGAGGTCAAAGGAACATTGATTTTGGTCCTTTCTGCGCAGCTTCCCCTGTCCCAAGTCCACGTTGACTTACAAAATAGGACCACTTTGATTTATAAACTCCCTCCATTCTTGATTTTGTATGATCTTCTTCCCCAAATAGACTGATCAGAAAGTAAGATCAGAGCGATGGCTGAGTTTCTATGGACTTTTGGAGTACAGGAAGTGTTGAAGAAGGTGGTGAAACTTGCAGCAGAGCAAATTGGCCTCACATGGGGCTTTGAGAAGGAGCTTTCGGAGCTAAGGGAATGGCTACTCAAGACTGAAACCTTCTTAACTGATATCAACACAAAAAAATTGCATCATTATTCAGTGAGGCTGTGGGTGGAGAAACTTCATCATCTTGTTCGTAAGGTCGACGATCTATTAGACAAACTTGTTTATGAAGATCTTCGACGGAAGGTGGAGACTGAAAAGATGAAGAAGGTACGCAATTTCATTTTCCCTACTAAGAATTATTTTGTTTTTCGTCACAAGATGGCAAAGAAAATAAAGAACATTATGGAACAATTAAGCAAACATGAGCATGAGGCAACTCGTTTAGGACTTGTTGGTGATGAATCCATAGAAAGGATGCAGAGTGATCTTAGCCAGATTCGTGAGACAGTCTCAGAGCCTGATGATTTTGAAGTTGTGGGAAGGGACGGGGAAGTTTCAAGCATAGTGGAACAGGTGATTGAAGCCAGCAATCGGCATGTTGTCACTATCTTACCTATTTTGGGTATGGGTGGATTGGGAAAAACAACTTTGGCGAGGTTGGTTTTCAATCATAAGATGATCAAAGAACATTTTAATGAAACTATATGGGTATGTGTGTCCGAACCTTTCGATGTCAACAAGATTTTGAGAGCAATCTTACAAAATCTAAAGGGTCCTTCAAGTAGCTGTTACACTAGGGAGGTCTTATTTCGTGAGCTTGAAAAGGAGATGCATGGTAAAAGATATTTTCTTGTGCTGGATGATGTTTGGAATGAAGATTCTTTTTTGTGGGAGCAATTGAAGAACTGTTTAATGAGAATCACTAGAAATTCTGAAAATAGTATTGTTGTGACTACAAGGAGGAGTGCTGAAGTTGTCAAAATCATGGAACAAACACTTCATTGTCATCATCTAAGCAAACTATCTGATGATCAATGTTGGTCCTTATTTGTGGAAAGCGCAAATGCTAGTGGAGTGCCTATGACTTCAAATTTGGAGGTTGTTCGAAAAGATTTGGTTAAAAAGTTTGGTGGTGTACCCTTAGTTCCTCGAGTTTTAGGAAGTGCAATAAGATTTCAAGAAAACTATGACAATTGGGTGACAATGTTGGAAAGTGTAATCAGAACTCCGCTACAGTTGCAAGAGAAAAATTTTGCTCTACCCATATTGAAATTAAGTGTGGATCATCTACCATCTTCATTAAAACAATGTTTTGCTTATTGTTCAAATTTTCCCAAAGATTTTGAGTTTAAAAAAGAACAACTAATTCAAATGTGGATGGCACAAGGATTTATTCAACTAGAAGAAGGAAGAAGCAACCAAACAATGGAAGATGAAGGAGATACGTACTTCAAGATCTTACTATCCCGTTGCTTATTTCAAGACGTTGTTAAAGACAATAGAGGGAGAATCATAAGTTGTAAGATGCATGATCTTATACATGATATTGCCTGTGATGTTTCAATGGATCAAAAATTGCAATTAGATCCTAGAAATTTATCTGACGAGGAACTTTGGACAAATAGGAGCAAAAGAATTGCAAGTAAGTTGCGCACGATAGTTTGTTATGAAGAGATGCCTCACAGAATAGAGAAGGTGATTCATCATAAGATTGCGAACTTTGTTTGTTTTTGCGTGTTTTCACTACAAGAAGGTGGAGTTGTTTCGACCACTTTTTATCGAAGGTTATTGAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACCTTTGAAAAATTTATGAATCTTTATCGAAGGTTTTTAGAAAACCTTAGAAAATTATGCAATTTTTATCTAAGGTTATAAAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACCTTTGAAAAATAGTACATTTATCAACAAATATTTAAAACATTTGAAAATATATGATATTTTATAGAAGGTTTTCAAACCTTCGAAAATGATTTTTTTTTATTTTTTTTATCAAAGGTTATTAAAAACCTTCGAAAAATATACAGAAAGTATCTCGATGATCTTCGAAAATTATTCACTTTTTTAACAAGGATTATTGAAAAACCTTTGAAAAATAGTTTAATATATTTATCGAAGATGTACTTAGAAAACTTAGAGAATTAAAAAAAAAGAGTTATGTACTTTTCATCACAGATTATTAAAAACCTTAAAAAATATGAAAACTATACCTGATAAGTTATTAACTTTTTTTTTAATCGAAGGGGCTAAAAAAGTTTGAATGATATTAGAAGGTATTTAAAAACTTTAAAAAACTAATTTTTTTCTTCTCTTTTTTTTTTGTGGGGAGTGACTAAAAACTTTTGAAAATATATAAAAAGATTTTTGATGGAAGGTTGTTAGATATATTTTTTTATCAACTTTTATCCAAGGTTACTAATAACCATCCAAAAAGATGTATAATATTTTTTAAAATAATATAATTGTCTGAAATAGTTTACCTATTTTCATCCTCAACTTACGATAGTCTTTCCCCTCTTCGCCAAACTTTGCCCTAGTTTATGCATTCGTGCCACCTCTGCCCGTTGCCGCCGAGTAGCCATCGCCGCCGCAAGAGTTCCTCCTCCATTGATTGTGGTTGTGGCACCTCAAATTGAGGTATGTCACTTAAATGTATGTTCACTCTCTTATACTCACTCAAATTCCATTGTTCTCTCAAGTTCCATCATTCTTTTTTTTCCATTTTTCAACGATTCTTCGTCAGAGATTTACGATTCTCCGTCATTAAGTTTCATATTCAATTCATCGAGAGAGCTGATGAATAATTCACTATCTCTTTAGATTGAGGTACGAAATTCTCTTTATATCAACCATAATCGTATTTTATCTTCAATCAACCTCTAGCACTTTCTTCCAATTTTGATTCTTAATTTGGACGTTTATTAACTTGTTTGGTGGTGCGTTTTTGCCGTTAGGTATTGCAAGCCGTTGAATTCTAATTAGCGATACGAATGGTGTTTCTTTCCCTGTAAGTACATAAAGTGAATTATCTGCTTGAATTGTGAAAAAATTTGGATTCCAGTTTAATTCTTCATCTTGAACCGTTTTCTGATTTTAATTTAGAATTTCTTTTTCCATATTAGCGCTCTGGTTTAGTTTTTCGAACATCTATAAGCTAAAGTTGGCATTAACTTTGAGACATATTAAGGATATAATATCTCACTAGAGGCTTGTATAGTAACCTTCCACGACCAAATATAAGTTATACAAACTCAAATTTTTAGGATTTATTTTGTCCTATAACCAAAATATATATTACACAAAACTTTAAGTTTCCTAATCTGCCATAAACTCAGTTTGTAATTTGCAAAAGGGAGTGATTGAGGAGTTAGATACCTTGTATTTGGTAACCAAATATAAGCTTGACTTTATAACTCAAGACTATACAAATCCAACTCGTGAATCAAAATGCTCTGACGAATAATCTTTTATGCAGAATGTCTGTGGAGGTAATTAGTTCATTTTATCATTTCTTTAATTTGATCCATGTTTTCAGTTTGTGAGTGGATTGTAATTATGTAATAGCCTGTTTAGTAACGTTTTTGTTTATTCTGCAGGCTTGTAATGCTCATGGCTTTATTGTGTTCCTTTATATGACACTCTGGGTAAAATGCTAAACTTTTTTCATTTTGGTCTTCAAACTTCAAAAACTTCAAATGGAAGAAAAAAATAGTTTTAGGCGTTGTTAATCTTTTGATTAATCATTTAATATAAAGAAGTACATTTTTGTTCATTTTTAAAGTGTTTGAAGTCAAATGGAGTAGGTTAGTAGCCATATTTTCTTTTTCCATCACATTGTCTTTTAATGAAACACTCCTTGTATGGTATTTAACAATTTTAGATGATGATCATTGTGTTATATAACAATTTCTTCTCTACTGTGATTTCAGTAGTATATTTGAACTTAGAACTCTTGCATGCTCTAACTTATCTTAAATTAATTGTTGTCATAAATATATAACATAAAGTGATGTTTGCTAATGAGTGAGAATTGAAAATTTTTATTTCTTCAAATTTGTGTTGGTTTTGTGTTTTTTGAAATTATAATAATGATTTTGAGTGTTAGCAGCACCAGAGAAAAGATAGTATGTTCCTTTTGCATATAACAGGTTCATCAAGTAAGTTGGTTGCTTAATTAATCACCCGATTTCTGCATTTATCTTATAAGTAATAGGATTCTTGATTTTAAAATTAAGATCTGGAAGGTGGTAGATACTTAGAATAGTTTGTTTGTACTTGCACTGATGGAATGTGATAAAAGTAGAACTACTAGTCATATCAAAATGAACTTGAGCAGACTCTCATACTTATGAGATACTTCATGTCTTTAGGTTCTGCTTGGTACAGACTCTCATACTTGTACAGCTGGTGCATTTGGTCAATTTGCTAGTGGAATTGGTAACACTGATGCATAA

mRNA sequence

ATGTCCCTAAGGGATGGAGCGACTAGGGCCTTGGGTACAACCTACCTTGAGTTCTCAACAGAGCAAATTGGCCTCACATGGGGCTTTGAGAAGGAGCTTTCGGAGCTAAGGGAATGGCTACTCAAGACTGAAACCTTCTTAACTGATATCAACACAAAAAAATTGCATCATTATTCAGTGAGGCTGTGGGTGGAGAAACTTCATCATCTTGTTCGTAAGGTCGACGATCTATTAGACAAACTTGTTTATGAAGATCTTCGACGGAAGGTGGAGACTGAAAAGATGAAGAAGGTACGCAATTTCATTTTCCCTACTAAGAATTATTTTGTTTTTCGTCACAAGATGGCAAAGAAAATAAAGAACATTATGGAACAATTAAGCAAACATGAGCATGAGGCAACTCGTTTAGGACTTGTTGGTGATGAATCCATAGAAAGGATGCAGAGTGATCTTAGCCAGATTCGTGAGACAGTCTCAGAGCCTGATGATTTTGAAGTTGTGGGAAGGGACGGGGAAGTTTCAAGCATAGTGGAACAGGTGATTGAAGCCAGCAATCGGCATGTTGTCACTATCTTACCTATTTTGGGTATGGGTGGATTGGGAAAAACAACTTTGGCGAGGTTGGTTTTCAATCATAAGATGATCAAAGAACATTTTAATGAAACTATATGGGTATGTGTGTCCGAACCTTTCGATGTCAACAAGATTTTGAGAGCAATCTTACAAAATCTAAAGGGTCCTTCAAGTAGCTGTTACACTAGGGAGGTCTTATTTCGTGAGCTTGAAAAGGAGATGCATGGTAAAAGATATTTTCTTGTGCTGGATGATGTTTGGAATGAAGATTCTTTTTTGTGGGAGCAATTGAAGAACTGTTTAATGAGAATCACTAGAAATTCTGAAAATAGTATTGTTGTGACTACAAGGAGGAGTGCTGAAGTTGTCAAAATCATGGAACAAACACTTCATTGTCATCATCTAAGCAAACTATCTGATGATCAATGTTGGTCCTTATTTGTGGAAAGCGCAAATGCTAGTGGAGTGCCTATGACTTCAAATTTGGAGGTTGTTCGAAAAGATTTGGTTAAAAAGTTTGGTGGTGTACCCTTAGTTCCTCGAGTTTTAGGAAGTGCAATAAGATTTCAAGAAAACTATGACAATTGGGTGACAATGTTGGAAAGTGTAATCAGAACTCCGCTACATTTATGCATTCGTGCCACCTCTGCCCGTTGCCGCCGAGTAGCCATCGCCGCCGCAAGAGTTCCTCCTCCATTGATTGTGGTTGTGGCACCTCAAATTGAGGTTCTGCTTGGTACAGACTCTCATACTTGTACAGCTGGTGCATTTGGTCAATTTGCTAGTGGAATTGGTAACACTGATGCATAA

Coding sequence (CDS)

ATGTCCCTAAGGGATGGAGCGACTAGGGCCTTGGGTACAACCTACCTTGAGTTCTCAACAGAGCAAATTGGCCTCACATGGGGCTTTGAGAAGGAGCTTTCGGAGCTAAGGGAATGGCTACTCAAGACTGAAACCTTCTTAACTGATATCAACACAAAAAAATTGCATCATTATTCAGTGAGGCTGTGGGTGGAGAAACTTCATCATCTTGTTCGTAAGGTCGACGATCTATTAGACAAACTTGTTTATGAAGATCTTCGACGGAAGGTGGAGACTGAAAAGATGAAGAAGGTACGCAATTTCATTTTCCCTACTAAGAATTATTTTGTTTTTCGTCACAAGATGGCAAAGAAAATAAAGAACATTATGGAACAATTAAGCAAACATGAGCATGAGGCAACTCGTTTAGGACTTGTTGGTGATGAATCCATAGAAAGGATGCAGAGTGATCTTAGCCAGATTCGTGAGACAGTCTCAGAGCCTGATGATTTTGAAGTTGTGGGAAGGGACGGGGAAGTTTCAAGCATAGTGGAACAGGTGATTGAAGCCAGCAATCGGCATGTTGTCACTATCTTACCTATTTTGGGTATGGGTGGATTGGGAAAAACAACTTTGGCGAGGTTGGTTTTCAATCATAAGATGATCAAAGAACATTTTAATGAAACTATATGGGTATGTGTGTCCGAACCTTTCGATGTCAACAAGATTTTGAGAGCAATCTTACAAAATCTAAAGGGTCCTTCAAGTAGCTGTTACACTAGGGAGGTCTTATTTCGTGAGCTTGAAAAGGAGATGCATGGTAAAAGATATTTTCTTGTGCTGGATGATGTTTGGAATGAAGATTCTTTTTTGTGGGAGCAATTGAAGAACTGTTTAATGAGAATCACTAGAAATTCTGAAAATAGTATTGTTGTGACTACAAGGAGGAGTGCTGAAGTTGTCAAAATCATGGAACAAACACTTCATTGTCATCATCTAAGCAAACTATCTGATGATCAATGTTGGTCCTTATTTGTGGAAAGCGCAAATGCTAGTGGAGTGCCTATGACTTCAAATTTGGAGGTTGTTCGAAAAGATTTGGTTAAAAAGTTTGGTGGTGTACCCTTAGTTCCTCGAGTTTTAGGAAGTGCAATAAGATTTCAAGAAAACTATGACAATTGGGTGACAATGTTGGAAAGTGTAATCAGAACTCCGCTACATTTATGCATTCGTGCCACCTCTGCCCGTTGCCGCCGAGTAGCCATCGCCGCCGCAAGAGTTCCTCCTCCATTGATTGTGGTTGTGGCACCTCAAATTGAGGTTCTGCTTGGTACAGACTCTCATACTTGTACAGCTGGTGCATTTGGTCAATTTGCTAGTGGAATTGGTAACACTGATGCATAA

Protein sequence

MSLRDGATRALGTTYLEFSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDLLDKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATRLGLVGDESIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILPILGMGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTREVLFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVKIMEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVLGSAIRFQENYDNWVTMLESVIRTPLHLCIRATSARCRRVAIAAARVPPPLIVVVAPQIEVLLGTDSHTCTAGAFGQFASGIGNTDA
Homology
BLAST of MS027905 vs. NCBI nr
Match: XP_022944235.1 (disease resistance protein RGA2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 554.7 bits (1428), Expect = 7.6e-154
Identity = 274/388 (70.62%), Postives = 332/388 (85.57%), Query Frame = 0

Query: 16  LEFSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVD 75
           +  + ++IGLTWG EKELS L+EWLLK+ETFL DINT+KLH+ SVRLWVEKLHHLV + D
Sbjct: 18  VRLAAKEIGLTWGLEKELSNLKEWLLKSETFLLDINTRKLHNDSVRLWVEKLHHLVHEAD 77

Query: 76  DLLDKLVYEDLRRKVE-TEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEAT 135
           DL+D LVYE LR KV+  +KM KV NF+FP+ N  VFR+KMAKK+ N M QL+KH+ EAT
Sbjct: 78  DLIDNLVYEHLRHKVQKNDKMNKVHNFLFPSNNSLVFRYKMAKKMNNFMNQLNKHDREAT 137

Query: 136 RLGLVGDESIER-MQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILP 195
           RLGLVGDE I+  +QSD+SQIRETVSE DDFEVVGRD EVSSIV+QVI+A N+ V +ILP
Sbjct: 138 RLGLVGDEPIQTLLQSDVSQIRETVSEVDDFEVVGRDVEVSSIVKQVIDAHNQRVTSILP 197

Query: 196 ILGMGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYT 255
           I+GMGGLGKTTLARL+FNH+MIK+HF+ETIWVCVS PFD+NKIL AILQN+KG     + 
Sbjct: 198 IVGMGGLGKTTLARLIFNHEMIKQHFDETIWVCVSIPFDINKILGAILQNVKGHDGCYHN 257

Query: 256 REVLFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEV 315
           RE+L REL+KEM+GKRYFLVLDDVWNEDS LWEQLKN LM+IT  S NSI+VTT R+ EV
Sbjct: 258 REILLRELKKEMYGKRYFLVLDDVWNEDSLLWEQLKNFLMKITSKSGNSILVTT-RNVEV 317

Query: 316 VKIMEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRV 375
            KIME+ L  HH++KLSD+QCWSLF+ESANA+G+P TSNLE VR++LVKKFGG+PLV RV
Sbjct: 318 CKIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVREELVKKFGGIPLVARV 377

Query: 376 LGSAIRFQENYDNWVTMLESVIRTPLHL 402
           LGSA++F+++YD WV++LE V+R+PLHL
Sbjct: 378 LGSAVKFEQDYDIWVSLLEGVLRSPLHL 404

BLAST of MS027905 vs. NCBI nr
Match: XP_022158717.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 505.4 bits (1300), Expect = 5.3e-139
Identity = 261/383 (68.15%), Postives = 312/383 (81.46%), Query Frame = 0

Query: 19  STEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDLL 78
           + EQIGL WG +KEL ELREWLLKTETFL DIN +KLHH SVRLWV+KL HLV + + LL
Sbjct: 21  AAEQIGLAWGLKKELHELREWLLKTETFLRDINKRKLHHDSVRLWVDKLQHLVHEAEHLL 80

Query: 79  DKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATRLGL 138
           D+LVYEDLRRKV T KMKKVR+F+ P+ N   FR KMA+K+KNI + L KH  EA  LGL
Sbjct: 81  DELVYEDLRRKVVTGKMKKVRDFVSPSNNILAFRVKMARKMKNITDLLCKHYCEAGPLGL 140

Query: 139 VGDESIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILPILGMG 198
           VGD S E ++ D++Q+RET+SE DDFEVVGRD EVS IV Q+I+A+N HV +ILPI+GMG
Sbjct: 141 VGDGSAE-IEIDIAQLRETISELDDFEVVGRDVEVSKIVNQLIDATNTHVTSILPIVGMG 200

Query: 199 GLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTREVLF 258
           GLGKTT+A++VFNH+ IK HF++TIWVCVSEPF VNKIL AIL  L+G SS    +E L 
Sbjct: 201 GLGKTTMAKVVFNHERIKTHFDKTIWVCVSEPFLVNKILGAILGTLRGTSSGLDNKEALL 260

Query: 259 RELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVKIME 318
            EL+KEMHGKRYFLVLDDVWNE   LW++LKNCLMRIT  SENSI+VTT RSAEV KIME
Sbjct: 261 HELKKEMHGKRYFLVLDDVWNESHSLWDELKNCLMRITERSENSILVTT-RSAEVAKIME 320

Query: 319 QTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVLGSAI 378
            TL  HHLSKLSDDQCWSLF+ESAN +G+ +TS+L+VV ++LVKK GG+PLV RVLG A+
Sbjct: 321 -TLPNHHLSKLSDDQCWSLFLESANVNGLSITSSLKVV-QELVKKIGGIPLVARVLGRAV 380

Query: 379 RFQENYDNWVTMLESVIRTPLHL 402
           +F+ +Y+ WVT LES+IRT L L
Sbjct: 381 KFEGDYETWVTTLESIIRTRLQL 399

BLAST of MS027905 vs. NCBI nr
Match: XP_022944234.1 (disease resistance protein RGA2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 505.4 bits (1300), Expect = 5.3e-139
Identity = 257/387 (66.41%), Postives = 312/387 (80.62%), Query Frame = 0

Query: 16  LEFSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVD 75
           +  + ++IGLTWG EKELS L+EWLLK+ETFL DINT+KLH+ SVRLWVEKLHHLV + D
Sbjct: 18  VRLAAKEIGLTWGLEKELSNLKEWLLKSETFLLDINTRKLHNDSVRLWVEKLHHLVHEAD 77

Query: 76  DLLDKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATR 135
           DL+D LVYE LR KV+              KN             + M +L+KH+ EATR
Sbjct: 78  DLIDNLVYEHLRHKVQ--------------KN-------------DKMNKLNKHDREATR 137

Query: 136 LGLVGDESIER-MQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILPI 195
           LGLVGDE I+  +QSD+SQIRETVSE DDFEVVGRD EVSSIV+QVI+A N+ V +ILPI
Sbjct: 138 LGLVGDEPIQTLLQSDVSQIRETVSEVDDFEVVGRDVEVSSIVKQVIDAHNQRVTSILPI 197

Query: 196 LGMGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTR 255
           +GMGGLGKTTLARL+FNH+MIK+HF+ETIWVCVS PFD+NKIL AILQN+KG     + R
Sbjct: 198 VGMGGLGKTTLARLIFNHEMIKQHFDETIWVCVSIPFDINKILGAILQNVKGHDGCYHNR 257

Query: 256 EVLFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVV 315
           E+L REL+KEM+GKRYFLVLDDVWNEDS LWEQLKN LM+IT  S NSI+VTT R+ EV 
Sbjct: 258 EILLRELKKEMYGKRYFLVLDDVWNEDSLLWEQLKNFLMKITSKSGNSILVTT-RNVEVC 317

Query: 316 KIMEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVL 375
           KIME+ L  HH++KLSD+QCWSLF+ESANA+G+P TSNLE VR++LVKKFGG+PLV RVL
Sbjct: 318 KIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVREELVKKFGGIPLVARVL 376

Query: 376 GSAIRFQENYDNWVTMLESVIRTPLHL 402
           GSA++F+++YD WV++LE V+R+PLHL
Sbjct: 378 GSAVKFEQDYDIWVSLLEGVLRSPLHL 376

BLAST of MS027905 vs. NCBI nr
Match: XP_022150842.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 503.8 bits (1296), Expect = 1.5e-138
Identity = 260/382 (68.06%), Postives = 311/382 (81.41%), Query Frame = 0

Query: 16  LEFSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVD 75
           ++ + EQIGL WG EK+L ELREWLLKTE FL DIN +KLH  SVRLWV+KL  LV + +
Sbjct: 18  VKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSDSVRLWVDKLQDLVHEAE 77

Query: 76  DLLDKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATR 135
           DLLD+LVYEDLRRKV+T KMKKVRNF+ P+ N FVFR KMA ++KNI + L KH HEA  
Sbjct: 78  DLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANRMKNISDLLCKHYHEAGS 137

Query: 136 LGLVGDESIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILPIL 195
           LGLV  ES E  + D+SQ+RETVSE DDF+VVGRD EVSSIV QV++A+NRHV +ILPI+
Sbjct: 138 LGLVASESAE-TEIDISQLRETVSELDDFDVVGRDVEVSSIVNQVMDATNRHVTSILPIV 197

Query: 196 GMGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTRE 255
           GMGGLGKTTLA+LVF+H+ I++HF++TIWVCVSEPF VNKIL AIL+ L+G S     +E
Sbjct: 198 GMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGAILETLRGTSDRLDNKE 257

Query: 256 VLFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVK 315
           VL  EL+KEMHGKRYFLVLDDVWNE+  LW++LKNCL RIT  SENSI+VTT RS EV K
Sbjct: 258 VLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEESENSILVTT-RSIEVAK 317

Query: 316 IMEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVLG 375
           IME TL  +HLSKLSDDQCW LF ESANA+G+PMTS+L+V ++ LVKK GGVPLV +VLG
Sbjct: 318 IME-TLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLVKKIGGVPLVAKVLG 377

Query: 376 SAIRFQENYDNWVTMLESVIRT 398
             ++FQ N+D WVT+LES I T
Sbjct: 378 RTVKFQGNFDIWVTILESEIST 396

BLAST of MS027905 vs. NCBI nr
Match: XP_038901555.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 469.2 bits (1206), Expect = 4.2e-128
Identity = 237/385 (61.56%), Postives = 301/385 (78.18%), Query Frame = 0

Query: 16  LEFSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVD 75
           L+ + EQIGL WGFE+ L  L++WLLK E FL DINT+K HH+SVR+WV+ L HLV + D
Sbjct: 18  LKVAGEQIGLAWGFEEHLYNLQKWLLKAEAFLRDINTRKFHHHSVRIWVDDLQHLVYEAD 77

Query: 76  DLLDKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATR 135
           DLLD++VYE LR+KV+T K KKV NF+ P+ N F+FR  MAKK+K ++E L KH +EA  
Sbjct: 78  DLLDEIVYEHLRQKVQTSKTKKVCNFVSPSNNAFIFRRNMAKKMKTVIELLEKHYYEAAP 137

Query: 136 LGLVGDESIERMQSDLSQIRETVSEP-DDFEVVGRDGEVSSIVEQVIEASNRHVVTILPI 195
           LGLV +ES       +SQ RET SE   D E+VGRD EV +IV+QVI+ASN+ + +ILPI
Sbjct: 138 LGLVRNESAGPEIDVISQFRETTSEVIGDHEIVGRDVEVENIVKQVIDASNQQLTSILPI 197

Query: 196 LGMGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTR 255
           +GMGGLGKT +A+L+ NH+MI+ HF++T+WVCVSEPF VN+ILR ILQNLKG S+   ++
Sbjct: 198 VGMGGLGKTAVAKLLINHEMIRGHFHKTVWVCVSEPFIVNEILREILQNLKGISNGGDSK 257

Query: 256 EVLFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVV 315
           EVL REL+K MHG+RYFLVLDDVWNE++FLW++LK CL+RIT NSEN IVVTT RSA+V 
Sbjct: 258 EVLLRELQKAMHGQRYFLVLDDVWNENAFLWDELKYCLLRITGNSENCIVVTT-RSADVA 317

Query: 316 KIMEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVL 375
           KIM+     +HLSKLSDDQCWSLF ESANA G+PMTS+LE+ +K LVKK GG+PL  RVL
Sbjct: 318 KIMKTHHPSYHLSKLSDDQCWSLFKESANARGLPMTSSLEIFQKKLVKKIGGLPLAARVL 377

Query: 376 GSAIRFQENYDNWVTMLESVIRTPL 400
           G A++F+ +   W  ML+SV+ T L
Sbjct: 378 GGAVKFEGDVKRWEEMLDSVLITRL 401

BLAST of MS027905 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 219.5 bits (558), Expect = 7.6e-56
Identity = 141/379 (37.20%), Postives = 208/379 (54.88%), Query Frame = 0

Query: 18  FSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDL 77
           F   ++GL +GFEKE  +L       +  L D   K+L + +++ W++KL+    +VDD+
Sbjct: 16  FIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDI 75

Query: 78  LDKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATRLG 137
           LD    E  R K      + V     P      F +K+ K++K +ME+L     E     
Sbjct: 76  LDDCKTEAARFK------QAVLGRYHP--RTITFCYKVGKRMKEMMEKLDAIAEERRNFH 135

Query: 138 LVGDESIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVI-EASNRHVVTILPILG 197
           L  DE I   Q+   Q    ++EP   +V GR+ E   IV+ +I   S    V +LPILG
Sbjct: 136 L--DERIIERQAARRQTGFVLTEP---KVYGREKEEDEIVKILINNVSYSEEVPVLPILG 195

Query: 198 MGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTREV 257
           MGGLGKTTLA++VFN + I EHFN  IWVCVS+ FD  ++++AI+++++G S        
Sbjct: 196 MGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDLAP 255

Query: 258 LFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVKI 317
           L ++L++ ++GKRYFLVLDDVWNED   W+ L+  L      S  SI++TTR   E +  
Sbjct: 256 LQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLK--IGASGASILITTR--LEKIGS 315

Query: 318 MEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVLGS 377
           +  TL  + LS LS + CW LF + A       +  L  + K++VKK GGVPL  + LG 
Sbjct: 316 IMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKCGGVPLAAKTLGG 375

Query: 378 AIRFQENYDNWVTMLESVI 396
            +RF+     W  + +S I
Sbjct: 376 LLRFKREESEWEHVRDSEI 377

BLAST of MS027905 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 210.7 bits (535), Expect = 3.5e-53
Identity = 131/379 (34.56%), Postives = 211/379 (55.67%), Query Frame = 0

Query: 18  FSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDL 77
           F   ++ L +GF+ E   L       +  L D   K+L+   +  W++KL+    +VDD+
Sbjct: 16  FLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAATYEVDDI 75

Query: 78  LDKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATRLG 137
           LD+  Y+    +    +  +    + P      FRHK+ K++  +M++L+    E  +  
Sbjct: 76  LDE--YKTKATRFLQSEYGRYHPKVIP------FRHKVGKRMDQVMKKLNAIAEERKKFH 135

Query: 138 LVGDESIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIE-ASNRHVVTILPILG 197
           L  ++ IER     +  RET S   + +V GRD E   IV+ +I  AS+   +++LPILG
Sbjct: 136 L-QEKIIERQ----AATRETGSVLTEPQVYGRDKEKDEIVKILINTASDAQKLSVLPILG 195

Query: 198 MGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTREV 257
           MGGLGKTTL+++VFN + + E F   IW+C+S+ F+  ++++AI+++++G S S      
Sbjct: 196 MGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGKSLSDMDLAP 255

Query: 258 LFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVKI 317
           L ++L++ ++GKRYFLVLDDVWNED   W  L+  L      +  + V+TT R  +V  I
Sbjct: 256 LQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVL---KVGASGAFVLTTTRLEKVGSI 315

Query: 318 MEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVLGS 377
           M  TL  + LS LS + CW LF++ A      +  NL  + K++VKK GGVPL  + LG 
Sbjct: 316 M-GTLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKKCGGVPLAAKTLGG 375

Query: 378 AIRFQENYDNWVTMLESVI 396
            +RF+     W  + +S I
Sbjct: 376 ILRFKREEREWEHVRDSPI 377

BLAST of MS027905 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.9e-51
Identity = 130/380 (34.21%), Postives = 206/380 (54.21%), Query Frame = 0

Query: 18  FSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDL 77
           F   ++ L +GF+ E   L       +  L D   K+L++  +  W++KL+    +VDD+
Sbjct: 16  FLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAATYEVDDI 75

Query: 78  LDKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATRLG 137
           LD+  Y+    +    +  +    + P      FRHK+ K++  +M++L     E     
Sbjct: 76  LDE--YKTKATRFSQSEYGRYHPKVIP------FRHKVGKRMDQVMKKLKAIAEERKNFH 135

Query: 138 LVGDESIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVI-EASNRHVVTILPILG 197
           L   E I   Q+   +    ++EP   +V GRD E   IV+ +I   S+   +++LPILG
Sbjct: 136 L--HEKIVERQAVRRETGSVLTEP---QVYGRDKEKDEIVKILINNVSDAQHLSVLPILG 195

Query: 198 MGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKG-PSSSCYTRE 257
           MGGLGKTTLA++VFN + + EHF+  IW+CVSE FD  ++++AI+++++G P        
Sbjct: 196 MGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEGRPLLGEMDLA 255

Query: 258 VLFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVK 317
            L ++L++ ++GKRY LVLDDVWNED   W  L+  L      +  + V+TT R  +V  
Sbjct: 256 PLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVL---KVGASGASVLTTTRLEKVGS 315

Query: 318 IMEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVLG 377
           IM  TL  + LS LS + CW LF++ A      +  NL  + K++VKK GGVPL  + LG
Sbjct: 316 IM-GTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKKSGGVPLAAKTLG 375

Query: 378 SAIRFQENYDNWVTMLESVI 396
             + F+     W  + +S I
Sbjct: 376 GILCFKREERAWEHVRDSPI 378

BLAST of MS027905 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.1e-49
Identity = 129/371 (34.77%), Postives = 198/371 (53.37%), Query Frame = 0

Query: 18  FSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDL 77
           F  +++ L +GFEKE  +L       +  L D   K+L   ++  W++KL+    +VDD+
Sbjct: 16  FIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSAAYEVDDI 75

Query: 78  LDKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATRLG 137
           L +   E +R   E  ++     F  P      FRHK+ +++K IME+L     E  +  
Sbjct: 76  LGECKNEAIR--FEQSRL----GFYHP--GIINFRHKIGRRMKEIMEKLDAISEERRKFH 135

Query: 138 LVGDESIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASN-RHVVTILPILG 197
            +  E I   Q+  +  RET     + +V GRD E   IV+ +I   N    + + PI+G
Sbjct: 136 FL--EKITERQA-AAATRETGFVLTEPKVYGRDKEEDEIVKILINNVNVAEELPVFPIIG 195

Query: 198 MGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTREV 257
           MGGLGKTTLA+++FN + + +HFN  IWVCVS+ FD  ++++ I+ N++  S        
Sbjct: 196 MGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERSSPHVEDLAS 255

Query: 258 LFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVKI 317
             ++L++ ++GKRY LVLDDVWN+D   W +L+  L    R +  SI+ TTR   E V  
Sbjct: 256 FQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGARGA--SILATTR--LEKVGS 315

Query: 318 MEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVLGS 377
           +  TL  +HLS LS      LF++ A         NL  + K++VKK GGVPL  + LG 
Sbjct: 316 IMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKKCGGVPLAAKTLGG 371

Query: 378 AIRFQENYDNW 388
            +RF+     W
Sbjct: 376 LLRFKREESEW 371

BLAST of MS027905 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.3e-34
Identity = 107/372 (28.76%), Postives = 188/372 (50.54%), Query Frame = 0

Query: 30  EKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDLLDKLVYEDLRRK 89
           E  L  L   LL     L D   K++ +  V  WV +L  +V   +D LD +  E LR  
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 90  VETEKMKKVR-NFIFPTKNYFVFRHKMAKKIKNIMEQLS-KHEHEATRLGLVGDESIERM 149
           +  E     R   +    +   F    ++ ++  +E+++ + E  A++  ++G + +  M
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAM 155

Query: 150 QSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVI-EASNRHVVTILPILGMGGLGKTTLA 209
                Q   T S  D+ EV GRD +   I+  +I E    + +T++ I+G+GG+GKTTL+
Sbjct: 156 IP--KQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLS 215

Query: 210 RLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTREVLFRELEKEMH 269
           +L++N + ++ +F   +W  VSE FDV KI + + +++          +VL  +L++ + 
Sbjct: 216 QLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLT 275

Query: 270 GK--RYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVKIMEQTLHCH 329
           G    + LVLDD+WNE+   W+ L+   +   + S+   ++ T RS  V  IM   +H H
Sbjct: 276 GTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQ---ILVTTRSQRVASIM-CAVHVH 335

Query: 330 HLSKLSDDQCWSLFVESANASGVP-MTSNLEVVRKDLVKKFGGVPLVPRVLGSAIRFQEN 389
           +L  LSD  CWSLF+++   +  P +   +  + + +V K  G+PL  + LG  +RF+  
Sbjct: 336 NLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGK 395

Query: 390 YDNWVTMLESVI 396
              W  +L S I
Sbjct: 396 VIEWERVLSSRI 401

BLAST of MS027905 vs. ExPASy TrEMBL
Match: A0A6J1FYJ5 (disease resistance protein RGA2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448744 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 3.7e-154
Identity = 274/388 (70.62%), Postives = 332/388 (85.57%), Query Frame = 0

Query: 16  LEFSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVD 75
           +  + ++IGLTWG EKELS L+EWLLK+ETFL DINT+KLH+ SVRLWVEKLHHLV + D
Sbjct: 18  VRLAAKEIGLTWGLEKELSNLKEWLLKSETFLLDINTRKLHNDSVRLWVEKLHHLVHEAD 77

Query: 76  DLLDKLVYEDLRRKVE-TEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEAT 135
           DL+D LVYE LR KV+  +KM KV NF+FP+ N  VFR+KMAKK+ N M QL+KH+ EAT
Sbjct: 78  DLIDNLVYEHLRHKVQKNDKMNKVHNFLFPSNNSLVFRYKMAKKMNNFMNQLNKHDREAT 137

Query: 136 RLGLVGDESIER-MQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILP 195
           RLGLVGDE I+  +QSD+SQIRETVSE DDFEVVGRD EVSSIV+QVI+A N+ V +ILP
Sbjct: 138 RLGLVGDEPIQTLLQSDVSQIRETVSEVDDFEVVGRDVEVSSIVKQVIDAHNQRVTSILP 197

Query: 196 ILGMGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYT 255
           I+GMGGLGKTTLARL+FNH+MIK+HF+ETIWVCVS PFD+NKIL AILQN+KG     + 
Sbjct: 198 IVGMGGLGKTTLARLIFNHEMIKQHFDETIWVCVSIPFDINKILGAILQNVKGHDGCYHN 257

Query: 256 REVLFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEV 315
           RE+L REL+KEM+GKRYFLVLDDVWNEDS LWEQLKN LM+IT  S NSI+VTT R+ EV
Sbjct: 258 REILLRELKKEMYGKRYFLVLDDVWNEDSLLWEQLKNFLMKITSKSGNSILVTT-RNVEV 317

Query: 316 VKIMEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRV 375
            KIME+ L  HH++KLSD+QCWSLF+ESANA+G+P TSNLE VR++LVKKFGG+PLV RV
Sbjct: 318 CKIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVREELVKKFGGIPLVARV 377

Query: 376 LGSAIRFQENYDNWVTMLESVIRTPLHL 402
           LGSA++F+++YD WV++LE V+R+PLHL
Sbjct: 378 LGSAVKFEQDYDIWVSLLEGVLRSPLHL 404

BLAST of MS027905 vs. ExPASy TrEMBL
Match: A0A6J1DXY1 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111025175 PE=4 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 2.6e-139
Identity = 261/383 (68.15%), Postives = 312/383 (81.46%), Query Frame = 0

Query: 19  STEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDLL 78
           + EQIGL WG +KEL ELREWLLKTETFL DIN +KLHH SVRLWV+KL HLV + + LL
Sbjct: 21  AAEQIGLAWGLKKELHELREWLLKTETFLRDINKRKLHHDSVRLWVDKLQHLVHEAEHLL 80

Query: 79  DKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATRLGL 138
           D+LVYEDLRRKV T KMKKVR+F+ P+ N   FR KMA+K+KNI + L KH  EA  LGL
Sbjct: 81  DELVYEDLRRKVVTGKMKKVRDFVSPSNNILAFRVKMARKMKNITDLLCKHYCEAGPLGL 140

Query: 139 VGDESIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILPILGMG 198
           VGD S E ++ D++Q+RET+SE DDFEVVGRD EVS IV Q+I+A+N HV +ILPI+GMG
Sbjct: 141 VGDGSAE-IEIDIAQLRETISELDDFEVVGRDVEVSKIVNQLIDATNTHVTSILPIVGMG 200

Query: 199 GLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTREVLF 258
           GLGKTT+A++VFNH+ IK HF++TIWVCVSEPF VNKIL AIL  L+G SS    +E L 
Sbjct: 201 GLGKTTMAKVVFNHERIKTHFDKTIWVCVSEPFLVNKILGAILGTLRGTSSGLDNKEALL 260

Query: 259 RELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVKIME 318
            EL+KEMHGKRYFLVLDDVWNE   LW++LKNCLMRIT  SENSI+VTT RSAEV KIME
Sbjct: 261 HELKKEMHGKRYFLVLDDVWNESHSLWDELKNCLMRITERSENSILVTT-RSAEVAKIME 320

Query: 319 QTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVLGSAI 378
            TL  HHLSKLSDDQCWSLF+ESAN +G+ +TS+L+VV ++LVKK GG+PLV RVLG A+
Sbjct: 321 -TLPNHHLSKLSDDQCWSLFLESANVNGLSITSSLKVV-QELVKKIGGIPLVARVLGRAV 380

Query: 379 RFQENYDNWVTMLESVIRTPLHL 402
           +F+ +Y+ WVT LES+IRT L L
Sbjct: 381 KFEGDYETWVTTLESIIRTRLQL 399

BLAST of MS027905 vs. ExPASy TrEMBL
Match: A0A6J1FWC6 (disease resistance protein RGA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448744 PE=4 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 2.6e-139
Identity = 257/387 (66.41%), Postives = 312/387 (80.62%), Query Frame = 0

Query: 16  LEFSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVD 75
           +  + ++IGLTWG EKELS L+EWLLK+ETFL DINT+KLH+ SVRLWVEKLHHLV + D
Sbjct: 18  VRLAAKEIGLTWGLEKELSNLKEWLLKSETFLLDINTRKLHNDSVRLWVEKLHHLVHEAD 77

Query: 76  DLLDKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATR 135
           DL+D LVYE LR KV+              KN             + M +L+KH+ EATR
Sbjct: 78  DLIDNLVYEHLRHKVQ--------------KN-------------DKMNKLNKHDREATR 137

Query: 136 LGLVGDESIER-MQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILPI 195
           LGLVGDE I+  +QSD+SQIRETVSE DDFEVVGRD EVSSIV+QVI+A N+ V +ILPI
Sbjct: 138 LGLVGDEPIQTLLQSDVSQIRETVSEVDDFEVVGRDVEVSSIVKQVIDAHNQRVTSILPI 197

Query: 196 LGMGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTR 255
           +GMGGLGKTTLARL+FNH+MIK+HF+ETIWVCVS PFD+NKIL AILQN+KG     + R
Sbjct: 198 VGMGGLGKTTLARLIFNHEMIKQHFDETIWVCVSIPFDINKILGAILQNVKGHDGCYHNR 257

Query: 256 EVLFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVV 315
           E+L REL+KEM+GKRYFLVLDDVWNEDS LWEQLKN LM+IT  S NSI+VTT R+ EV 
Sbjct: 258 EILLRELKKEMYGKRYFLVLDDVWNEDSLLWEQLKNFLMKITSKSGNSILVTT-RNVEVC 317

Query: 316 KIMEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVL 375
           KIME+ L  HH++KLSD+QCWSLF+ESANA+G+P TSNLE VR++LVKKFGG+PLV RVL
Sbjct: 318 KIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVREELVKKFGGIPLVARVL 376

Query: 376 GSAIRFQENYDNWVTMLESVIRTPLHL 402
           GSA++F+++YD WV++LE V+R+PLHL
Sbjct: 378 GSAVKFEQDYDIWVSLLEGVLRSPLHL 376

BLAST of MS027905 vs. ExPASy TrEMBL
Match: A0A6J1DBV6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111018890 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 7.5e-139
Identity = 260/382 (68.06%), Postives = 311/382 (81.41%), Query Frame = 0

Query: 16  LEFSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVD 75
           ++ + EQIGL WG EK+L ELREWLLKTE FL DIN +KLH  SVRLWV+KL  LV + +
Sbjct: 18  VKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSDSVRLWVDKLQDLVHEAE 77

Query: 76  DLLDKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATR 135
           DLLD+LVYEDLRRKV+T KMKKVRNF+ P+ N FVFR KMA ++KNI + L KH HEA  
Sbjct: 78  DLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANRMKNISDLLCKHYHEAGS 137

Query: 136 LGLVGDESIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILPIL 195
           LGLV  ES E  + D+SQ+RETVSE DDF+VVGRD EVSSIV QV++A+NRHV +ILPI+
Sbjct: 138 LGLVASESAE-TEIDISQLRETVSELDDFDVVGRDVEVSSIVNQVMDATNRHVTSILPIV 197

Query: 196 GMGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTRE 255
           GMGGLGKTTLA+LVF+H+ I++HF++TIWVCVSEPF VNKIL AIL+ L+G S     +E
Sbjct: 198 GMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGAILETLRGTSDRLDNKE 257

Query: 256 VLFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVK 315
           VL  EL+KEMHGKRYFLVLDDVWNE+  LW++LKNCL RIT  SENSI+VTT RS EV K
Sbjct: 258 VLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEESENSILVTT-RSIEVAK 317

Query: 316 IMEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVLG 375
           IME TL  +HLSKLSDDQCW LF ESANA+G+PMTS+L+V ++ LVKK GGVPLV +VLG
Sbjct: 318 IME-TLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLVKKIGGVPLVAKVLG 377

Query: 376 SAIRFQENYDNWVTMLESVIRT 398
             ++FQ N+D WVT+LES I T
Sbjct: 378 RTVKFQGNFDIWVTILESEIST 396

BLAST of MS027905 vs. ExPASy TrEMBL
Match: A0A1S3CHQ5 (disease resistance protein RGA2-like OS=Cucumis melo OX=3656 GN=LOC103501070 PE=4 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.9e-126
Identity = 237/385 (61.56%), Postives = 303/385 (78.70%), Query Frame = 0

Query: 16  LEFSTEQIGLTWGFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVD 75
           L+ + EQ GL WGF+K LS+L++WLLK E FL DINT+KLHH SVR+WV+ L HLV + D
Sbjct: 18  LKVAQEQTGLAWGFQKHLSKLQKWLLKAEAFLRDINTRKLHHDSVRMWVDDLRHLVYQAD 77

Query: 76  DLLDKLVYEDLRRKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATR 135
           DLLD++VYE LR+KV T KMKKV +F  P+ N F+FR  MA K+  ++E L KH +EA  
Sbjct: 78  DLLDEIVYEHLRQKVHTRKMKKVCDFFSPSSNAFIFRRNMANKMMTLVELLEKHYNEAAP 137

Query: 136 LGLVGDESIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILPIL 195
           LGLV +E+       +SQ RET+SE +D ++VGRD EV SIV+QVI+ASN  + +ILPI+
Sbjct: 138 LGLVVNENARPEIDVISQYRETISELEDHKIVGRDVEVESIVKQVIDASNNQLTSILPIV 197

Query: 196 GMGGLGKTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGP-SSSCYTR 255
           GMGGLGKTTLA+LVFNH+++++HF++T+WVCVSEPF VNKIL  ILQNL+G  S+   ++
Sbjct: 198 GMGGLGKTTLAKLVFNHELVRQHFDKTVWVCVSEPFIVNKILLDILQNLQGGISNGGDSK 257

Query: 256 EVLFRELEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVV 315
           EVL REL+KEM G+ YFLVLDDVWNE+SFLW++LK CL++IT NS+NSIVVTT RS EV 
Sbjct: 258 EVLLRELQKEMLGQTYFLVLDDVWNENSFLWDELKYCLLKITGNSKNSIVVTT-RSVEVA 317

Query: 316 KIMEQTLHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVL 375
           KIM  T   H LSKLSDD CWSLF ESANA G+ MTSNL +++K+LVKK GGVPLV RVL
Sbjct: 318 KIM-GTCSGHLLSKLSDDYCWSLFKESANAYGLSMTSNLGIIQKELVKKIGGVPLVARVL 377

Query: 376 GSAIRFQENYDNWVTMLESVIRTPL 400
           G+A++F+ + + W  ML+SV RTP+
Sbjct: 378 GNAVKFEGDVERWKEMLKSVQRTPV 400

BLAST of MS027905 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 146.7 bits (369), Expect = 4.5e-35
Identity = 107/372 (28.76%), Postives = 188/372 (50.54%), Query Frame = 0

Query: 30  EKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDLLDKLVYEDLRRK 89
           E  L  L   LL     L D   K++ +  V  WV +L  +V   +D LD +  E LR  
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 90  VETEKMKKVR-NFIFPTKNYFVFRHKMAKKIKNIMEQLS-KHEHEATRLGLVGDESIERM 149
           +  E     R   +    +   F    ++ ++  +E+++ + E  A++  ++G + +  M
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAM 155

Query: 150 QSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVI-EASNRHVVTILPILGMGGLGKTTLA 209
                Q   T S  D+ EV GRD +   I+  +I E    + +T++ I+G+GG+GKTTL+
Sbjct: 156 IP--KQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLS 215

Query: 210 RLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTREVLFRELEKEMH 269
           +L++N + ++ +F   +W  VSE FDV KI + + +++          +VL  +L++ + 
Sbjct: 216 QLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLT 275

Query: 270 GK--RYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVKIMEQTLHCH 329
           G    + LVLDD+WNE+   W+ L+   +   + S+   ++ T RS  V  IM   +H H
Sbjct: 276 GTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQ---ILVTTRSQRVASIM-CAVHVH 335

Query: 330 HLSKLSDDQCWSLFVESANASGVP-MTSNLEVVRKDLVKKFGGVPLVPRVLGSAIRFQEN 389
           +L  LSD  CWSLF+++   +  P +   +  + + +V K  G+PL  + LG  +RF+  
Sbjct: 336 NLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGK 395

Query: 390 YDNWVTMLESVI 396
              W  +L S I
Sbjct: 396 VIEWERVLSSRI 401

BLAST of MS027905 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 141.7 bits (356), Expect = 1.4e-33
Identity = 108/374 (28.88%), Postives = 185/374 (49.47%), Query Frame = 0

Query: 29  FEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDLLDKLVYEDLRR 88
           + K+L +L+  L   ++FL D   +K  + ++R  V  L  LV + +D+L      D   
Sbjct: 27  YRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDCQLADGDD 86

Query: 89  KVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEA-------TRLGLVGD 148
             E        + + P +     ++K +K+++ I E+++K + +        T   +  D
Sbjct: 87  GNEQRSSNAWLSRLHPAR--VPLQYKKSKRLQEINERITKIKSQVEPYFEFITPSNVGRD 146

Query: 149 ESIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILPILGMGGLG 208
              +R  S +          D  +VVG +G+   I E +   SN   + I+  +GMGGLG
Sbjct: 147 NGTDRWSSPVY---------DHTQVVGLEGDKRKIKEWLFR-SNDSQLLIMAFVGMGGLG 206

Query: 209 KTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTREVLFREL 268
           KTT+A+ VFN K I+  F   IWV VS+ F   +I+R+IL+NL G +S       L R++
Sbjct: 207 KTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNL-GDASVGDDIGTLLRKI 266

Query: 269 EKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVKIMEQTL 328
           ++ + GKRY +V+DDVW+++   W+++      + R    S++VTTR  +   ++  +  
Sbjct: 267 QQYLLGKRYLIVMDDVWDKNLSWWDKI---YQGLPRGQGGSVIVTTRSESVAKRVQARDD 326

Query: 329 HCHHLSKLSDDQCWSLFVESANAS--GVPMTSNLEVVRKDLVKKFGGVPLVPRVLGSAIR 388
             H    LS D  W LF   A A+  G      LE V K++V K  G+PL  + +G  + 
Sbjct: 327 KTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVGGLLL 384

Query: 389 FQEN-YDNWVTMLE 393
            +++ Y  W  + E
Sbjct: 387 CKDHVYHEWRRIAE 384

BLAST of MS027905 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 141.7 bits (356), Expect = 1.4e-33
Identity = 108/374 (28.88%), Postives = 185/374 (49.47%), Query Frame = 0

Query: 29  FEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDLLDKLVYEDLRR 88
           + K+L +L+  L   ++FL D   +K  + ++R  V  L  LV + +D+L      D   
Sbjct: 27  YRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDCQLADGDD 86

Query: 89  KVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEA-------TRLGLVGD 148
             E        + + P +     ++K +K+++ I E+++K + +        T   +  D
Sbjct: 87  GNEQRSSNAWLSRLHPAR--VPLQYKKSKRLQEINERITKIKSQVEPYFEFITPSNVGRD 146

Query: 149 ESIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILPILGMGGLG 208
              +R  S +          D  +VVG +G+   I E +   SN   + I+  +GMGGLG
Sbjct: 147 NGTDRWSSPVY---------DHTQVVGLEGDKRKIKEWLFR-SNDSQLLIMAFVGMGGLG 206

Query: 209 KTTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTREVLFREL 268
           KTT+A+ VFN K I+  F   IWV VS+ F   +I+R+IL+NL G +S       L R++
Sbjct: 207 KTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNL-GDASVGDDIGTLLRKI 266

Query: 269 EKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVKIMEQTL 328
           ++ + GKRY +V+DDVW+++   W+++      + R    S++VTTR  +   ++  +  
Sbjct: 267 QQYLLGKRYLIVMDDVWDKNLSWWDKI---YQGLPRGQGGSVIVTTRSESVAKRVQARDD 326

Query: 329 HCHHLSKLSDDQCWSLFVESANAS--GVPMTSNLEVVRKDLVKKFGGVPLVPRVLGSAIR 388
             H    LS D  W LF   A A+  G      LE V K++V K  G+PL  + +G  + 
Sbjct: 327 KTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVGGLLL 384

Query: 389 FQEN-YDNWVTMLE 393
            +++ Y  W  + E
Sbjct: 387 CKDHVYHEWRRIAE 384

BLAST of MS027905 vs. TAIR 10
Match: AT5G35450.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 141.0 bits (354), Expect = 2.4e-33
Identity = 103/374 (27.54%), Postives = 178/374 (47.59%), Query Frame = 0

Query: 28  GFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDLLDKLVYEDLR 87
           G ++++  L+  L   ++ L D + KK     VR ++E +  LV   +D+++  V   LR
Sbjct: 26  GIDEQVDGLKRQLRGLQSLLKDADAKKHGSDRVRNFLEDVKDLVFDAEDIIESYVLNKLR 85

Query: 88  RKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATRLGLV-----GDE 147
                 + K V+N +     +   RHK+A  I+ I +++SK   E   LG+      G  
Sbjct: 86  -----GEGKGVKNHVRRLACFLTDRHKVASDIEGITKRISKVIGEMQSLGIQQQIIDGGR 145

Query: 148 SIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILPILGMGGLGK 207
           S+  +Q    +IR+T     + ++VG +  V  +V  ++E  N  VV+   I GMGG+GK
Sbjct: 146 SLS-LQDIQREIRQTFPNSSESDLVGVEQSVEELVGPMVEIDNIQVVS---ISGMGGIGK 205

Query: 208 TTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTRE--VLFRE 267
           TTLAR +F+H +++ HF+   WVCVS+ F    + + ILQ L+         +   +  +
Sbjct: 206 TTLARQIFHHDLVRRHFDGFAWVCVSQQFTQKHVWQRILQELRPHDGEILQMDEYTIQGK 265

Query: 268 LEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVKIMEQT 327
           L + +   RY +VLDDVW E+   W+++K    R         ++ T R+  V    + T
Sbjct: 266 LFQLLETGRYLVVLDDVWKEED--WDRIKEVFPR----KRGWKMLLTSRNEGVGLHADPT 325

Query: 328 LHCHHLSKLSDDQCWSLFVESANASGVPMTSNLEVVRKDLVKKFGGVPLVPRVLGSAIRF 387
                   L+  + W LF              +E + K++V   GG+PL  +VLG  +  
Sbjct: 326 CLSFRARILNPKESWKLFERIVPRRNETEYEEMEAIGKEMVTYCGGLPLAVKVLGGLLAN 384

Query: 388 QENYDNWVTMLESV 395
           +     W  + E++
Sbjct: 386 KHTASEWKRVSENI 384

BLAST of MS027905 vs. TAIR 10
Match: AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 136.0 bits (341), Expect = 7.9e-32
Identity = 103/376 (27.39%), Postives = 186/376 (49.47%), Query Frame = 0

Query: 28  GFEKELSELREWLLKTETFLTDINTKKLHHYSVRLWVEKLHHLVRKVDDLLDKLVYEDLR 87
           G ++++  L+  L + ++ L D + KK     VR ++E +  +V   DD+++  +  +LR
Sbjct: 26  GIDEQVDGLKRQLGRLQSLLKDADAKKNETERVRNFLEDVKDIVYDADDIIESFLLNELR 85

Query: 88  RKVETEKMKKVRNFIFPTKNYFVFRHKMAKKIKNIMEQLSKHEHEATRLGLV-----GDE 147
            K +  K K+VR        + V R K A  I+ I +++S+       LG+      G  
Sbjct: 86  GKEKGIK-KQVRTLAC----FLVDRRKFASDIEGITKRISEVIVGMQSLGIQHIADGGGR 145

Query: 148 SIERMQSDLSQIRETVSEPDDFEVVGRDGEVSSIVEQVIEASNRHVVTILPILGMGGLGK 207
           S+  +Q    +IR+T S   + ++VG D  V  +V+ ++E  +   V ++ + GMGG+GK
Sbjct: 146 SLS-LQERQREIRQTFSRNSESDLVGLDQSVEELVDHLVENDS---VQVVSVSGMGGIGK 205

Query: 208 TTLARLVFNHKMIKEHFNETIWVCVSEPFDVNKILRAILQNLKGPSSSCYTRE--VLFRE 267
           TTLAR VF+H +++ HF+   WVCVS+ F    + + ILQ+L+         +   L  E
Sbjct: 206 TTLARQVFHHDIVRRHFDGFSWVCVSQQFTRKDVWQRILQDLRPYDEGIIQMDEYTLQGE 265

Query: 268 LEKEMHGKRYFLVLDDVWNEDSFLWEQLKNCLMRITRNSENSIVVTTRRSAEVVKIMEQT 327
           L + +   RY LVLDDVW E+   W+++K     +  +     ++ T R+  +    + T
Sbjct: 266 LFELLESGRYLLVLDDVWKEED--WDRIK----AVFPHKRGWKMLLTSRNEGLGLHADPT 325

Query: 328 LHCHHLSKLSDDQCWSLFVESANASGVPMTSNL-EVVRKDLVKKFGGVPLVPRVLGSAIR 387
                   L+ +Q W LF    ++        + E + K++V   GG+PL  +VLG  + 
Sbjct: 326 CFAFRPRILTPEQSWKLFERIVSSRRDKTEFKVDEAMGKEMVTYCGGLPLAVKVLGGLLA 385

Query: 388 FQENYDNWVTMLESVI 396
            +     W  +  +++
Sbjct: 386 KKHTVLEWKRVHSNIV 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022944235.17.6e-15470.62disease resistance protein RGA2-like isoform X2 [Cucurbita moschata][more]
XP_022158717.15.3e-13968.15putative disease resistance protein RGA3 [Momordica charantia][more]
XP_022944234.15.3e-13966.41disease resistance protein RGA2-like isoform X1 [Cucurbita moschata][more]
XP_022150842.11.5e-13868.06putative disease resistance protein RGA3 [Momordica charantia][more]
XP_038901555.14.2e-12861.56putative disease resistance protein RGA3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q7XA407.6e-5637.20Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA423.5e-5334.56Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XBQ91.9e-5134.21Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Q7XA393.1e-4934.77Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q9LRR46.3e-3428.76Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A6J1FYJ53.7e-15470.62disease resistance protein RGA2-like isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1DXY12.6e-13968.15putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1FWC62.6e-13966.41disease resistance protein RGA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1DBV67.5e-13968.06putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A1S3CHQ51.9e-12661.56disease resistance protein RGA2-like OS=Cucumis melo OX=3656 GN=LOC103501070 PE=... [more]
Match NameE-valueIdentityDescription
AT3G14470.14.5e-3528.76NB-ARC domain-containing disease resistance protein [more]
AT3G50950.21.4e-3328.88HOPZ-ACTIVATED RESISTANCE 1 [more]
AT3G50950.11.4e-3328.88HOPZ-ACTIVATED RESISTANCE 1 [more]
AT5G35450.12.4e-3327.54Disease resistance protein (CC-NBS-LRR class) family [more]
AT1G53350.17.9e-3227.39Disease resistance protein (CC-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 112..132
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 264..278
score: 42.0
coord: 191..206
score: 55.78
coord: 364..378
score: 36.83
NoneNo IPR availableGENE3D1.20.5.4130coord: 10..125
e-value: 3.0E-10
score: 42.1
NoneNo IPR availablePANTHERPTHR23155:SF1055OS05G0414200 PROTEINcoord: 29..391
IPR041118Rx, N-terminalPFAMPF18052Rx_Ncoord: 16..97
e-value: 1.7E-11
score: 44.2
IPR015931Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3GENE3D3.30.499.10Aconitase, domain 3coord: 426..460
e-value: 9.2E-8
score: 33.5
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 173..399
e-value: 1.7E-42
score: 145.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 147..323
e-value: 3.5E-36
score: 126.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 152..399
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 328..403
e-value: 3.7E-5
score: 25.2
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 29..391
IPR038005Virus X resistance protein-like, coiled-coil domainCDDcd14798RX-CC_likecoord: 17..128
e-value: 6.16585E-8
score: 49.1575
IPR036008Aconitase, iron-sulfur domainSUPERFAMILY53732Aconitase iron-sulfur domaincoord: 428..460

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS027905.1MS027905.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding