MS027821 (gene) Bitter gourd (TR) v1

Overview
NameMS027821
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncytochrome P450 71A1-like
Locationscaffold22: 1007897 .. 1009545 (+)
RNA-Seq ExpressionMS027821
SyntenyMS027821
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCAGTTGTCAAAATTTCAGCAACATTGGCGACAACTTCAAGCTCCATATGGAGGCACCTTCCACTTCAATCTCATCTCTTTTTCTCTCTTCCTTTTCTTCTTCTTCTTCTTCCTTGTCTTAAGGAAGTTTTTCAGAGCCAAAAACACCAAAAATTTCATTCCCTCACCTCCCAAACTTCCCATCATCGGCAACCTCCACCAGTTAGGAACACTGCCACACCAATCTCTGGCAGCTCTTTCTAACAAATATGGGCCTCTCATGCTCCTCAAGCTGGGGCAGGCCCCAACTCTGGTGGTTTCCTCTGCAGAAATGGCTAGAGAAGTGATGAAGACACATGACATCAAATTCTGCAACAGGGTCCGAACTACGGCCGCAAAGATCTTGCTGTACGATTGCCAAGACATGGCTTTTGCCCCATATGGAGAGTTCTTGAGACAAGCCAAGAAAATGTGCGTTTTGGAGCTTTTAAGTGCCAAAAGAGTGGAGTCGTTTCAGTATGTGAGAGATGAGGAAGTTGAAGTGCTTATCAATAGGATTCGTGAAGGTTGTGTCGGTGGGGAGGAGGCCGTGAATCTCAAGCAACTGTTCGCGTCCATATCAAATAATATAATTTCGAGGTGTGTTTTTGGAGAGAAGTTTGAGGATGAAAATGGGAAGAGCGAATTTCGAGATGCGTCGAGAAGGATTCAAGTGTTAATTTCAACTTTTTGTGTTGCAGATTTCTTCCCTGCTTTTGGATGGGTTGATGTACTTAGAGGGTTCATTGGAGAGCTGAAAAAGATTTCTAAAAAAATGGATGAATTCTTTGATGAGATCATTGAAGAACACAAGACAAAGATGAGTAAAAGTGGTCAATCTTCTTATGGTAAAAAGGATTTTGTGGATATCTTGTTGCAACTACAGCAGGATGATACGCTCAACTATCATTTCACTCGAGATGAACTCAAAGCAATCTTACAGGTTTTTCTCAGATCTCTCTTGCCTATACACACAAACACAAGAGAAGCAATTTAAATTGAGCCAATTTTGTTTTCAAATTGCAGGACATGTTCATAGCTGGGAGCGACACTACTGCAACAGCTATGGAATGGACAATGACTGAGCTGATGAGAAATCAGGCCGCCATGAAGAAAGTCCAAGAAGAGGTCAGAAAAATAATTGGAAAGAATCCGAAGTTTGAAATGGAAGATACTGGAAAGATGGAGTACCTGCACTGTGTGGTAAAGGAATCTCTAAGACTACATCCACCTGCTCCTTTACTAGTGCCTCGACAAACATTGGGAGATGTCAAGATTGGAGGCTACCACATCCCATCAAAAACCAACGTTTTTGTGAATGTTTGGGCAATTCAAAGGGACCCCCAGATTTGGGAAAGGCCACATGAGTTCATCCCAGAGAGATTCACAGAGAACTCAATTGATTACAAAGGTCATTACTTTGAGTTCATTCCATTTGGGAGCGGGAGAAGGAAATGCTCTGGAATTTTATTCGGGATTACTTCCATTGAATTTGTGTTGGCAAATCTTCTCTATTGGTTTGATTGGGAGCTTCCTGATGGCTGTGTTTTGGATGCTGAAGAAGAGAATGGGATAACCGTTTGCAAGAAAATTCCCCTCCATCTCAAGCCAATGCCATACCTCTAG

mRNA sequence

ATGGATCAGTTGTCAAAATTTCAGCAACATTGGCGACAACTTCAAGCTCCATATGGAGGCACCTTCCACTTCAATCTCATCTCTTTTTCTCTCTTCCTTTTCTTCTTCTTCTTCTTCCTTGTCTTAAGGAAGTTTTTCAGAGCCAAAAACACCAAAAATTTCATTCCCTCACCTCCCAAACTTCCCATCATCGGCAACCTCCACCAGTTAGGAACACTGCCACACCAATCTCTGGCAGCTCTTTCTAACAAATATGGGCCTCTCATGCTCCTCAAGCTGGGGCAGGCCCCAACTCTGGTGGTTTCCTCTGCAGAAATGGCTAGAGAAGTGATGAAGACACATGACATCAAATTCTGCAACAGGGTCCGAACTACGGCCGCAAAGATCTTGCTGTACGATTGCCAAGACATGGCTTTTGCCCCATATGGAGAGTTCTTGAGACAAGCCAAGAAAATGTGCGTTTTGGAGCTTTTAAGTGCCAAAAGAGTGGAGTCGTTTCAGTATGTGAGAGATGAGGAAGTTGAAGTGCTTATCAATAGGATTCGTGAAGGTTGTGTCGGTGGGGAGGAGGCCGTGAATCTCAAGCAACTGTTCGCGTCCATATCAAATAATATAATTTCGAGGTGTGTTTTTGGAGAGAAGTTTGAGGATGAAAATGGGAAGAGCGAATTTCGAGATGCGTCGAGAAGGATTCAAGTGTTAATTTCAACTTTTTGTGTTGCAGATTTCTTCCCTGCTTTTGGATGGGTTGATGTACTTAGAGGGTTCATTGGAGAGCTGAAAAAGATTTCTAAAAAAATGGATGAATTCTTTGATGAGATCATTGAAGAACACAAGACAAAGATGAGTAAAAGTGGTCAATCTTCTTATGGTAAAAAGGATTTTGTGGATATCTTGTTGCAACTACAGCAGGATGATACGCTCAACTATCATTTCACTCGAGATGAACTCAAAGCAATCTTACAGGACATGTTCATAGCTGGGAGCGACACTACTGCAACAGCTATGGAATGGACAATGACTGAGCTGATGAGAAATCAGGCCGCCATGAAGAAAGTCCAAGAAGAGGTCAGAAAAATAATTGGAAAGAATCCGAAGTTTGAAATGGAAGATACTGGAAAGATGGAGTACCTGCACTGTGTGGTAAAGGAATCTCTAAGACTACATCCACCTGCTCCTTTACTAGTGCCTCGACAAACATTGGGAGATGTCAAGATTGGAGGCTACCACATCCCATCAAAAACCAACGTTTTTGTGAATGTTTGGGCAATTCAAAGGGACCCCCAGATTTGGGAAAGGCCACATGAGTTCATCCCAGAGAGATTCACAGAGAACTCAATTGATTACAAAGGTCATTACTTTGAGTTCATTCCATTTGGGAGCGGGAGAAGGAAATGCTCTGGAATTTTATTCGGGATTACTTCCATTGAATTTGTGTTGGCAAATCTTCTCTATTGGTTTGATTGGGAGCTTCCTGATGGCTGTGTTTTGGATGCTGAAGAAGAGAATGGGATAACCGTTTGCAAGAAAATTCCCCTCCATCTCAAGCCAATGCCATACCTCTAG

Coding sequence (CDS)

ATGGATCAGTTGTCAAAATTTCAGCAACATTGGCGACAACTTCAAGCTCCATATGGAGGCACCTTCCACTTCAATCTCATCTCTTTTTCTCTCTTCCTTTTCTTCTTCTTCTTCTTCCTTGTCTTAAGGAAGTTTTTCAGAGCCAAAAACACCAAAAATTTCATTCCCTCACCTCCCAAACTTCCCATCATCGGCAACCTCCACCAGTTAGGAACACTGCCACACCAATCTCTGGCAGCTCTTTCTAACAAATATGGGCCTCTCATGCTCCTCAAGCTGGGGCAGGCCCCAACTCTGGTGGTTTCCTCTGCAGAAATGGCTAGAGAAGTGATGAAGACACATGACATCAAATTCTGCAACAGGGTCCGAACTACGGCCGCAAAGATCTTGCTGTACGATTGCCAAGACATGGCTTTTGCCCCATATGGAGAGTTCTTGAGACAAGCCAAGAAAATGTGCGTTTTGGAGCTTTTAAGTGCCAAAAGAGTGGAGTCGTTTCAGTATGTGAGAGATGAGGAAGTTGAAGTGCTTATCAATAGGATTCGTGAAGGTTGTGTCGGTGGGGAGGAGGCCGTGAATCTCAAGCAACTGTTCGCGTCCATATCAAATAATATAATTTCGAGGTGTGTTTTTGGAGAGAAGTTTGAGGATGAAAATGGGAAGAGCGAATTTCGAGATGCGTCGAGAAGGATTCAAGTGTTAATTTCAACTTTTTGTGTTGCAGATTTCTTCCCTGCTTTTGGATGGGTTGATGTACTTAGAGGGTTCATTGGAGAGCTGAAAAAGATTTCTAAAAAAATGGATGAATTCTTTGATGAGATCATTGAAGAACACAAGACAAAGATGAGTAAAAGTGGTCAATCTTCTTATGGTAAAAAGGATTTTGTGGATATCTTGTTGCAACTACAGCAGGATGATACGCTCAACTATCATTTCACTCGAGATGAACTCAAAGCAATCTTACAGGACATGTTCATAGCTGGGAGCGACACTACTGCAACAGCTATGGAATGGACAATGACTGAGCTGATGAGAAATCAGGCCGCCATGAAGAAAGTCCAAGAAGAGGTCAGAAAAATAATTGGAAAGAATCCGAAGTTTGAAATGGAAGATACTGGAAAGATGGAGTACCTGCACTGTGTGGTAAAGGAATCTCTAAGACTACATCCACCTGCTCCTTTACTAGTGCCTCGACAAACATTGGGAGATGTCAAGATTGGAGGCTACCACATCCCATCAAAAACCAACGTTTTTGTGAATGTTTGGGCAATTCAAAGGGACCCCCAGATTTGGGAAAGGCCACATGAGTTCATCCCAGAGAGATTCACAGAGAACTCAATTGATTACAAAGGTCATTACTTTGAGTTCATTCCATTTGGGAGCGGGAGAAGGAAATGCTCTGGAATTTTATTCGGGATTACTTCCATTGAATTTGTGTTGGCAAATCTTCTCTATTGGTTTGATTGGGAGCTTCCTGATGGCTGTGTTTTGGATGCTGAAGAAGAGAATGGGATAACCGTTTGCAAGAAAATTCCCCTCCATCTCAAGCCAATGCCATACCTCTAG

Protein sequence

MDQLSKFQQHWRQLQAPYGGTFHFNLISFSLFLFFFFFFLVLRKFFRAKNTKNFIPSPPKLPIIGNLHQLGTLPHQSLAALSNKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYDCQDMAFAPYGEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVLINRIREGCVGGEEAVNLKQLFASISNNIISRCVFGEKFEDENGKSEFRDASRRIQVLISTFCVADFFPAFGWVDVLRGFIGELKKISKKMDEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDTLNYHFTRDELKAILQDMFIAGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKIIGKNPKFEMEDTGKMEYLHCVVKESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQIWERPHEFIPERFTENSIDYKGHYFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDWELPDGCVLDAEEENGITVCKKIPLHLKPMPYL
Homology
BLAST of MS027821 vs. NCBI nr
Match: XP_038895465.1 (cytochrome P450 71A1-like [Benincasa hispida])

HSP 1 Score: 753.4 bits (1944), Expect = 1.3e-213
Identity = 373/511 (72.99%), Postives = 424/511 (82.97%), Query Frame = 0

Query: 15  QAPYGGTFHFNLISFSLFLFFFFFFLVLRKFFRA-KNTKNFIPSPPKLPIIGNLHQLGTL 74
           QAPYGGT HFNLISFSL    FFFFL+  K FR  K    F PSPPKLPIIGNLHQLG L
Sbjct: 3   QAPYGGTLHFNLISFSL---CFFFFLIFLKLFRGNKKITKFPPSPPKLPIIGNLHQLGAL 62

Query: 75  PHQSLAALSNKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYD 134
           PHQSLAALS KYGPLM LKLGQ PTLVVSS +MARE+MKTHD+KF NR +TT  K LLY 
Sbjct: 63  PHQSLAALSKKYGPLMFLKLGQTPTLVVSSPKMAREIMKTHDLKFSNRPQTTPGKHLLYG 122

Query: 135 CQDMAFAPYGEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVLINRI-REGC--VGGEE 194
           CQD+AF+PYGE+ RQAKKMCVLELLSAKRVE+FQYVRDEEV +LI+RI R+ C  + G+E
Sbjct: 123 CQDLAFSPYGEYWRQAKKMCVLELLSAKRVEAFQYVRDEEVGLLIDRIRRKSCDVICGDE 182

Query: 195 AVNLKQLFASISNNIISRCVFGEKFEDENGKSEFRDASRRIQVLISTFCVADFFPAFGWV 254
            VNLKQ+F S + NIISRCV GEKFEDENG+S F D SRRI VLI+ F V DFFP+FGW+
Sbjct: 183 CVNLKQMFMSTTTNIISRCVLGEKFEDENGESRFGDVSRRILVLITAFSVGDFFPSFGWI 242

Query: 255 DVLRGFIGELKKISKKMDEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDTLNY 314
           DV+RGFI ELK  SK +D FFD+IIEEH+TKMS     S  +KDFVDI+LQLQQDD  +Y
Sbjct: 243 DVVRGFIRELKTTSKILDGFFDKIIEEHRTKMSVG--KSDEEKDFVDIMLQLQQDDMFDY 302

Query: 315 H-FTRDELKAILQDMFIAGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKIIGKNPKFEM 374
           H F+ D LKAIL DMF+ GSDTTAT++EW MTELMRN AAMKKVQEE+R I+G  PK EM
Sbjct: 303 HDFSLDRLKAILLDMFVGGSDTTATSLEWAMTELMRNPAAMKKVQEEIRTIVGNKPKIEM 362

Query: 375 EDTGKMEYLHCVVKESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQ 434
           ED  KMEY+HCV+KE+LRLHPPAP LVPR++ G+V+I GYHIPSKT VFVNVWAIQRDP 
Sbjct: 363 EDIEKMEYMHCVIKETLRLHPPAPFLVPRESAGEVEIEGYHIPSKTRVFVNVWAIQRDPM 422

Query: 435 IWERPHEFIPERFTENSIDYKGHYFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDW 494
           IWE+P+EF+PERF +NSIDYKGH FEFIPFGSGRRKC+GI F ITS EF LAN+LYWFDW
Sbjct: 423 IWEKPNEFVPERFIQNSIDYKGHDFEFIPFGSGRRKCAGISFAITSFEFALANVLYWFDW 482

Query: 495 ELPDGCVLDAEEENGITVCKKIPLHLKPMPY 521
           +LP G VLD +EENG+T+CKK PLHLKP+PY
Sbjct: 483 KLPHGSVLDVDEENGLTICKKKPLHLKPVPY 508

BLAST of MS027821 vs. NCBI nr
Match: XP_022137055.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 716.1 bits (1847), Expect = 2.2e-202
Identity = 355/521 (68.14%), Postives = 410/521 (78.69%), Query Frame = 0

Query: 1   MDQLSKFQQHWRQLQAPYGGTFHFNLISFSLFLFFFFFFLVLRKFFRAK-NTKNFIPSPP 60
           MD +S F Q W+QL        HF+LI FSL L   FFFLVL K F+ K NTKNF PSPP
Sbjct: 1   MDLISNFLQFWQQL--------HFDLIPFSLCL--IFFFLVLLKLFKPKNNTKNFPPSPP 60

Query: 61  KLPIIGNLHQLGTLPHQSLAALSNKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFC 120
           KLPIIGNLHQLG+LPHQSLAALS+KYGPLMLLKLG A TLVVSSA+MARE+MKTHD+KF 
Sbjct: 61  KLPIIGNLHQLGSLPHQSLAALSDKYGPLMLLKLGHAQTLVVSSAKMARELMKTHDLKFS 120

Query: 121 NRVRTTAAKILLYDCQDMAFAPYGEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVLIN 180
           NR +T  AK LLY  +D+ FAPYGE+ RQA+K+C LEL SAKRVESFQYVRDEEV VL+N
Sbjct: 121 NRPQTMPAKTLLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVSVLVN 180

Query: 181 RIREGCV--GGEEAVNLKQLFASISNNIISRCVFGEKFEDENGKSEFRDASRRIQVLIST 240
           RIR+GCV   GE +VNL QLF S SNNI+SRC+ GEKFEDENGKS F D SRR+ VLI+ 
Sbjct: 181 RIRKGCVDGDGEGSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITA 240

Query: 241 FCVADFFPAFGWVDVLRGFIGELKKISKKMDEFFDEIIEEHKTKMSKSGQSSYGKKDFVD 300
           FC ADFFPA  W+DV+RGF GELKK    +D F  +++EEHK KM   G+S   KKDFV 
Sbjct: 241 FCFADFFPALWWIDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVG 300

Query: 301 ILLQLQQDDTLNYHFTRDELKAILQDMFIAGSDTTATAMEWTMTELMRNQAAMKKVQEEV 360
           I+LQL Q D ++YHF+ + LK I+ DMF+ G+DTTAT +EWTMTELMRN   M+KVQEEV
Sbjct: 301 IMLQLMQSDMVDYHFSLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEV 360

Query: 361 RKIIGKNPKFEMEDTGKMEYLHCVVKESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNV 420
           RK +GK  K E+ED  KM Y++CV+KESLRLHPP PLLVPR+ + DV+I GYHIPSKT V
Sbjct: 361 RKTVGKKAKIEIEDIRKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRV 420

Query: 421 FVNVWAIQRDPQIWERPHEFIPERFTENSIDYKGHYFEFIPFGSGRRKCSGILFGITSIE 480
           FVN W I RDP IWERP+EFIPERF ENS+DYKG  FEFIPFGSGRR C G+ FGI S E
Sbjct: 421 FVNAWKIHRDPTIWERPNEFIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFE 480

Query: 481 FVLANLLYWFDWELPDGCVLDAEEENGITVCKKIPLHLKPM 519
           + LANLLYWFDW+LP+G  LD EEENG+TV KK PLHL P+
Sbjct: 481 YALANLLYWFDWKLPNGFELDVEEENGLTVRKKNPLHLNPI 511

BLAST of MS027821 vs. NCBI nr
Match: XP_022923856.1 (cytochrome P450 71A1-like [Cucurbita moschata])

HSP 1 Score: 714.5 bits (1843), Expect = 6.5e-202
Identity = 359/523 (68.64%), Postives = 413/523 (78.97%), Query Frame = 0

Query: 1   MDQLSKFQQHWRQLQAPYGGTFHFNLISFSLFLFFFFFFLVLRKFFRAKNTKNFIPSPPK 60
           MD+ S F QHW++ Q  YG   H N IS S  LFFFF  L+L+ F   K + NF PSPPK
Sbjct: 1   MDRTSYF-QHWKRQQFQYGVAIHINPISISFCLFFFFLLLLLKLFRGKKQSINFPPSPPK 60

Query: 61  LPIIGNLHQLGTLPHQSLAALSNKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFCN 120
           LPIIGNLHQLGTLPHQSLA+LS KYGPLMLLKLGQ PTLVVSSA+MAREVM+THD+KF N
Sbjct: 61  LPIIGNLHQLGTLPHQSLASLSKKYGPLMLLKLGQTPTLVVSSAKMAREVMRTHDLKFSN 120

Query: 121 RVRTTAAKILLYDCQDMAFAPYGEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVLINR 180
           R +TTA  +L Y CQDM FAPYG + RQA+KMCVLEL S KRVESFQY+RDEEV VLI+R
Sbjct: 121 RPKTTATNLLFYGCQDMGFAPYGAYWRQARKMCVLELFSFKRVESFQYIRDEEVGVLISR 180

Query: 181 IREGCVGGEEAVNLKQLFASISNNIISRCVFGEKFEDENGKSEFRDASRRIQVLISTFCV 240
           I + C GG E VNL +LF  ISNNI+SRCV G+KFEDENG + FRD SRR+  L+S FCV
Sbjct: 181 IHKACAGG-EVVNLSKLFLQISNNIVSRCVMGKKFEDENGNNRFRDVSRRVIELLSAFCV 240

Query: 241 ADFFPAFGWVDVLRGFIGELKKISKKMDEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILL 300
           ADFFPAFGWVDV+RGFIGELK IS+ MDEF D +IEEHKT++ ++G+    K+DF+DI+L
Sbjct: 241 ADFFPAFGWVDVVRGFIGELKTISRTMDEFLDMVIEEHKTEL-RTGRPD-DKRDFLDIML 300

Query: 301 QLQQDDTLNYHFTRDELKAILQDMFIAGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKI 360
           QL QDD L YHFTRD LKAILQDMF  GSDT+ATA+EW M EL+RN   +KKVQEE+R I
Sbjct: 301 QLNQDDMLGYHFTRDNLKAILQDMFAGGSDTSATALEWAMAELIRNPNTLKKVQEEIRTI 360

Query: 361 IGKNPKFEMEDTGKMEYLHCVVKESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVN 420
           +GK PK EM D  KMEY+ CV+KESLRLHPP PLLVPR+T   V I GYH+ S T+VFVN
Sbjct: 361 VGKKPKIEMNDISKMEYMKCVIKESLRLHPPIPLLVPRETSDVVDIEGYHVASGTSVFVN 420

Query: 421 VWAIQRDPQIWERPHEFIPERFTE-NSIDYKGHYFEFIPFGSGRRKCSGILFGITSIEFV 480
            WAIQRDP IWERP+EFIPERF E N  D+KG  FEFIPFGSGRRKC G+ F + S E V
Sbjct: 421 AWAIQRDPIIWERPNEFIPERFMEKNCADFKGLDFEFIPFGSGRRKCPGLSFALASFECV 480

Query: 481 LANLLYWFDWELPD---GCVLDAEEENGITVCKKIPLHLKPMP 520
           LANLLYWFDW+LP+   G +LD  E +GITV KKIPLHL P+P
Sbjct: 481 LANLLYWFDWKLPEDMAGELLDMSELHGITVRKKIPLHLTPLP 519

BLAST of MS027821 vs. NCBI nr
Match: XP_022137054.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 712.6 bits (1838), Expect = 2.5e-201
Identity = 360/527 (68.31%), Postives = 416/527 (78.94%), Query Frame = 0

Query: 1   MDQLSKFQQHWRQLQAPYGGTFHFNLISFSLFLFFFFFFLVLRKFFRAK-NTKNFIPSPP 60
           MD +SKF+Q W+Q          F+LI FSL +  FFFFLVL K  + K NTKNF PSPP
Sbjct: 1   MDPISKFEQIWQQ----------FDLIPFSLCI--FFFFLVLLKLLKPKNNTKNFPPSPP 60

Query: 61  KLPIIGNLHQLGTLPHQSLAALSNKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFC 120
           KLPIIGNLHQLG+LPHQSLAALS+K+GPLM LKLGQAPTLVVSSAEMAREVMKTHD+ F 
Sbjct: 61  KLPIIGNLHQLGSLPHQSLAALSDKHGPLMFLKLGQAPTLVVSSAEMAREVMKTHDLNFS 120

Query: 121 NRVRTTAAKILLYDCQDMAFAPYGEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVLIN 180
           +R +TTAAKILLY  +D+ FAPYGE+ RQA+K+C LEL SAKRVESFQYVRDEEV VL+N
Sbjct: 121 DRPQTTAAKILLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVN 180

Query: 181 RIREGCVG--GEEAVNLKQLFASISNNIISRCVFGEKFEDENGKSEFRDASRRIQVLIST 240
           RIR GCVG  GE +VNL QLF S SNNI+SRC+ G+KFEDENGKS F D SRR+ VL++ 
Sbjct: 181 RIRRGCVGGDGEGSVNLAQLFLSTSNNIVSRCMMGDKFEDENGKSRFGDISRRVMVLLTA 240

Query: 241 FCVADFFPAFGWVDVLRGFIGELKKISKKMDEFFDEIIEEHKTKMSKSGQ-SSYGKKDFV 300
           FC ADFFPA  W+DV+RGF GELKK    +D FF +++EEHK KM   G+     K+DF+
Sbjct: 241 FCFADFFPALWWIDVVRGFNGELKKCFTTLDAFFSKVVEEHKAKMIGGGELCDESKRDFM 300

Query: 301 DILLQL-QQDDTLNYHFTRDELKAILQDMFIAGSDTTATAMEWTMTELMRNQAAMKKVQE 360
           D +LQL  QDD ++Y F+ D LKAI+ DMF+ GSDTTAT +EWTMTELMRN + MKKVQE
Sbjct: 301 DSMLQLILQDDMVDYRFSLDNLKAIVLDMFVGGSDTTATGLEWTMTELMRNPSVMKKVQE 360

Query: 361 EVRKIIGKNPKFEMEDTGKMEYLHCVVKESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKT 420
           EVRKI+G   K EMED   M Y++CV+KESLRLHPP PLLVPR+ +GDV+I GYHIPSKT
Sbjct: 361 EVRKIVGNKEKIEMEDIQNMPYMNCVIKESLRLHPPVPLLVPREAIGDVEIEGYHIPSKT 420

Query: 421 NVFVNVWAIQRDPQIWERPHEFIPERFTENSIDYKGHYFEFIPFGSGRRKCSGILFGITS 480
            VFVN W I RDP IWERP+EFIPERF ENSIDYKG  F+FIPFGSGRR C G  FGI S
Sbjct: 421 RVFVNAWKIHRDPNIWERPNEFIPERFMENSIDYKGQDFDFIPFGSGRRICPGKSFGIAS 480

Query: 481 IEFVLANLLYWFDWELPDGC-VLDAEEENGITVCKKIPLHLKPMPYL 522
            E+ LANLLYWFDW+LP G   LD EEENG+TV KKIPLHL P+ Y+
Sbjct: 481 FEYALANLLYWFDWKLPHGSKELDVEEENGLTVRKKIPLHLNPISYI 515

BLAST of MS027821 vs. NCBI nr
Match: XP_023520088.1 (cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 712.2 bits (1837), Expect = 3.2e-201
Identity = 358/523 (68.45%), Postives = 412/523 (78.78%), Query Frame = 0

Query: 1   MDQLSKFQQHWRQLQAPYGGTFHFNLISFSLFLFFFFFFLVLRKFFRAKNTKNFIPSPPK 60
           MD+ S F QHW++ Q  YG   HFN IS S  LFFFF  L+L+ F   K + NF PSPPK
Sbjct: 1   MDRTSNF-QHWKRQQFQYGEAIHFNPISISFCLFFFFLLLLLKLFRGKKQSINFPPSPPK 60

Query: 61  LPIIGNLHQLGTLPHQSLAALSNKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFCN 120
           LPIIGNLHQLGTLPHQSLA+LS KYGPLMLLKLGQ PTLVVSSA+MAREVMKTHD+KF N
Sbjct: 61  LPIIGNLHQLGTLPHQSLASLSKKYGPLMLLKLGQTPTLVVSSAKMAREVMKTHDLKFSN 120

Query: 121 RVRTTAAKILLYDCQDMAFAPYGEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVLINR 180
           R +TTA  +L Y CQDM FAPYG + RQA+KMCVLEL S KRVESFQY+RDEEV VLI+R
Sbjct: 121 RPKTTATNLLFYGCQDMGFAPYGAYWRQARKMCVLELFSFKRVESFQYIRDEEVGVLISR 180

Query: 181 IREGCVGGEEAVNLKQLFASISNNIISRCVFGEKFEDENGKSEFRDASRRIQVLISTFCV 240
           I + C GG E VNL +LF  ISNNI+SRCV G+KFEDENG + FRD SRR+  L+S FCV
Sbjct: 181 IHKACAGG-EVVNLSKLFLQISNNIVSRCVMGKKFEDENGNNRFRDVSRRVIELLSAFCV 240

Query: 241 ADFFPAFGWVDVLRGFIGELKKISKKMDEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILL 300
           ADFFPAFGWVDV+RGFIGELK IS+ MDEF D +IEEHKT++ ++G+    K+DF+DI+L
Sbjct: 241 ADFFPAFGWVDVVRGFIGELKTISRTMDEFLDRVIEEHKTEL-RTGKPD-DKRDFLDIML 300

Query: 301 QLQQDDTLNYHFTRDELKAILQDMFIAGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKI 360
           QL QD  L+YHFT D LKAILQDMF  GSDT+ATA+EW M EL+RN   +KKVQEE+R I
Sbjct: 301 QLNQDIMLDYHFTPDNLKAILQDMFAGGSDTSATALEWAMAELIRNPNTLKKVQEEIRTI 360

Query: 361 IGKNPKFEMEDTGKMEYLHCVVKESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVN 420
           +GK PK EM D  KMEY+ CV+KESLRLHPP PLLVPR+T   V I GYH+ S T+VFVN
Sbjct: 361 VGKKPKIEMNDISKMEYMKCVIKESLRLHPPIPLLVPRETSDVVDIEGYHVASGTSVFVN 420

Query: 421 VWAIQRDPQIWERPHEFIPERFTE-NSIDYKGHYFEFIPFGSGRRKCSGILFGITSIEFV 480
            WAIQRDP IWERP+EFIPERF E N  D+KG  FEFIPFGSGRRKC G+ F + S E V
Sbjct: 421 AWAIQRDPTIWERPNEFIPERFMEKNCADFKGLDFEFIPFGSGRRKCPGLSFALASFECV 480

Query: 481 LANLLYWFDWELPD---GCVLDAEEENGITVCKKIPLHLKPMP 520
           LANLLYWFDW+LP+   G +LD  E +GITV KK PLHL P+P
Sbjct: 481 LANLLYWFDWKLPEDMAGELLDMSELHGITVRKKNPLHLTPLP 519

BLAST of MS027821 vs. ExPASy Swiss-Prot
Match: A0A068Q609 (Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP71AN24 PE=1 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 3.9e-149
Identity = 271/529 (51.23%), Postives = 359/529 (67.86%), Query Frame = 0

Query: 1   MDQLSKFQQHWR--QLQAPYGGTFHFNLISFSLFLFFFFFFLVLRKFFRAKNTK-NFIPS 60
           M  L+ F Q W+  QLQ+    +F+  L+       F  F L        KN K    PS
Sbjct: 1   MALLTLFNQIWQEGQLQSS-TSSFNIFLVPILCLSIFILFSLTRSSSPSEKNRKLKLPPS 60

Query: 61  PPKLPIIGNLHQLGTLPHQSLAALSNKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIK 120
           PP+LP IGNLHQLG+ PH+SL ALS KYG +M +  G+ PTL+VSSAEMA++VMKT DI 
Sbjct: 61  PPRLPWIGNLHQLGSFPHRSLRALSKKYGDVMFMHFGKVPTLIVSSAEMAKDVMKTQDIV 120

Query: 121 FCNRVRTTAAKILLYDCQDMAFAPYGEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVL 180
           FC+R +TTA  IL YD  D+AFAPYGE+ RQ +++CVLELLS KRV  FQY R EEV  L
Sbjct: 121 FCSRPQTTAPSILFYDGHDIAFAPYGEYWRQVRRICVLELLSLKRVHQFQYARVEEVAEL 180

Query: 181 INRIREGCVGGEEA-VNLKQLFASISNNIISRCVFGEKFEDENGKSEFRDASRRIQVLIS 240
           +++IR+       A +NL +L  S SNNII RC+ G+KFED+   + F + ++ +   + 
Sbjct: 181 VSKIRKASASANGAPINLGELLVSTSNNIICRCILGQKFEDKE-DNWFGETTKELMTQVM 240

Query: 241 TFCVADFFPAFGWVDVLRGFIGELKKISKKMDEFFDEIIEEHKTKMSKSGQSSYGKKDFV 300
           +F   DFFP+  W+D  RG++  LK I  + D+FFD++I+EHK    K G+    KKD V
Sbjct: 241 SFSFGDFFPSLKWIDRARGYLAYLKSIWLEFDKFFDKLIDEHKA-AQKEGKPR--KKDIV 300

Query: 301 DILLQLQQDDTLNYHFTRDELKAILQDMFIAGSDTTATAMEWTMTELMRNQAAMKKVQEE 360
           DILL +Q D +L++  T   +KAILQDMF+ GSDT+ TA  W M+EL +N   MKKVQEE
Sbjct: 301 DILLDVQNDGSLDFELTTSNVKAILQDMFVGGSDTSWTAAIWLMSELSQNPRVMKKVQEE 360

Query: 361 VRKIIGKNPKFEMEDTGKMEYLHCVVKESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKTN 420
           VR++ GK    E  D  +M+YL CV+KE+LRLHPPAPLL+PR+ + DVK+GG+ IP+KT 
Sbjct: 361 VRRVAGKRGYVEESDINEMKYLTCVIKENLRLHPPAPLLLPREAMSDVKLGGFDIPAKTQ 420

Query: 421 VFVNVWAIQRDPQIWERPHEFIPERFTENSIDYKGHYFEFIPFGSGRRKCSGILFGITSI 480
           VFVN +A+QRDP++W++P EF+PERF EN++ + G  FE IPFG+GRR C G+ FG+ S 
Sbjct: 421 VFVNAYAVQRDPKVWDKPDEFMPERFEENNVGFVGQDFELIPFGAGRRVCPGLAFGVASA 480

Query: 481 EFVLANLLYWFDWELPDGC-----VLDAEEENGITVCKKIPLHLKPMPY 521
           ++VLAN+LYWFDW+LP G       LD  E  G+TV KK PL+L P PY
Sbjct: 481 QYVLANMLYWFDWKLPSGGSKLAETLDMSEVYGLTVHKKSPLYLVPTPY 524

BLAST of MS027821 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 515.4 bits (1326), Expect = 7.6e-145
Identity = 251/489 (51.33%), Postives = 338/489 (69.12%), Query Frame = 0

Query: 31  LFLFFFFFFLVLRKFFRAKNTKNFIPSPPKLPIIGNLHQLGTLPHQSLAALSNKYGPLML 90
           LFL     F +L+   + +   N  PSPP LPIIGNLHQLG LPH+SL +L+N+ GPL+L
Sbjct: 8   LFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLIL 67

Query: 91  LKLGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYDCQDMAFAPYGEFLRQAK 150
           L LG  PTL+VS+AE+A E++KTHD+ F +R  TTAA+ + YDC D+AF+PYGE+ RQ +
Sbjct: 68  LHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVR 127

Query: 151 KMCVLELLSAKRVESFQYVRDEEVEVLINRIREGCVGGEEAVNLKQLFASISNNIISRCV 210
           K+CVLELLS KRV S++ +R+EEV +++ RI + C  G EAVNL +L   +S+  I+R  
Sbjct: 128 KICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTG-EAVNLSELLLLLSSGTITRVA 187

Query: 211 FGEKFE-DENGKSEFRDASRRIQVLISTFCVADFFPAFGWVDVLRGFIGELKKISKKMDE 270
           FG+K+E +E  K++F D +  +  L+  F V D+FP+F WVDVL G    LK+   ++D 
Sbjct: 188 FGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDA 247

Query: 271 FFDEIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDTLNYHFTRDELKAILQDMFIAGS 330
           F D +I++H      +G     +KD VD+LL LQ+D +L  H  R+ LKA++ DMF  G+
Sbjct: 248 FVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSGGT 307

Query: 331 DTTATAMEWTMTELMRNQAAMKKVQEEVRKIIGKNPKFEMEDTGKMEYLHCVVKESLRLH 390
           DTTA  +EW M EL+++   M+K Q+EVR+++GK  K E ED  ++ YL  ++KE+LRLH
Sbjct: 308 DTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLRLH 367

Query: 391 PPAPLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQIWERPHEFIPERFTENSIDY 450
           P APLLVPR++  DV I GYHIP+KT VF+N WAI RDP+ WE   EF+PERF  NS+D+
Sbjct: 368 PVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNNSVDF 427

Query: 451 KGHYFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDWELPDGCV---LDAEEENGIT 510
           KG  F+ IPFG+GRR C GI FGI+S+E  LANLLYWF+WELP       LD  E  GIT
Sbjct: 428 KGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEAVGIT 487

Query: 511 VCKKIPLHL 516
           V  K PL L
Sbjct: 488 VHMKFPLQL 495

BLAST of MS027821 vs. ExPASy Swiss-Prot
Match: A0A0N9HTU1 (Desmethylyatein synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71CU1 PE=1 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 5.1e-125
Identity = 231/492 (46.95%), Postives = 312/492 (63.41%), Query Frame = 0

Query: 30  SLFLFFFFFFLVLRKFFRAKNTKNFIPSPPKLPIIGNLHQLGTLPHQSLAALSNKYGPLM 89
           +LFL F     +L++ F  K   N  PSPPKLPI+GN HQLGTL H+++  L+ KYGPLM
Sbjct: 8   TLFLLFISTVFILKRKFSHK--PNLPPSPPKLPILGNFHQLGTLVHRAVTVLAAKYGPLM 67

Query: 90  LLKLGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYDCQDMAFAPYGEFLRQA 149
           LL  G+ P L+VSS E A+E+MKTHD+   NR  TTAA+ LLYDC D++FAPYGE+ R+ 
Sbjct: 68  LLHFGKTPVLIVSSQETAKEIMKTHDLALANRPLTTAARALLYDCTDISFAPYGEYWREM 127

Query: 150 KKMCVLELLSAKRVESFQYVRDEEVEVLINRIREGCVGGEEAVNLKQLFASISNNIISRC 209
           KKM VL LLS K+++SF+ VR+E    +I  I      G   V++  +    S +++ RC
Sbjct: 128 KKMAVLNLLSIKKIQSFRSVREELASDMIKEITRLSKTG-APVDVTNMLYHFSEDLLFRC 187

Query: 210 VFGEKFEDENGKSEFRDASRRIQVLISTFCVADFFPAFGWVDVLRGFIGELKKISKKMDE 269
             G K     G+ +F+  SR    L+  FC  DFFP   W+D L G   +LKK S+++D+
Sbjct: 188 TLGFK---PKGQHKFQKLSRDFLDLVGAFCFNDFFPGMAWMDALTGLNRKLKKGSRELDD 247

Query: 270 FFDEIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDTLNYHFTRDELKAILQDMFIAGS 329
           F DE+IEE +  M K G       +F+D+LL   +D T     TRD +KAI+ D F+ G 
Sbjct: 248 FVDELIEE-RIAMVKDGVE---PNEFLDLLLHTHRDTTQEIKLTRDNVKAIILDTFLGGI 307

Query: 330 DTTATAMEWTMTELMRNQAAMKKVQEEVRKIIGKNPKFEMEDTGKMEYLHCVVKESLRLH 389
           D  A+ MEW M ELMRN + MK  QEEVRK++G   K + +D  +M +L   VKE+LRLH
Sbjct: 308 DLPASVMEWAMAELMRNPSKMKIAQEEVRKVVGNKNKVDEDDVYQMNFLKSAVKETLRLH 367

Query: 390 PPAPLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQIWERPHEFIPERFTENSIDY 449
           PPAPLL  R++   + +  Y IP  T+V +N+W IQRDP++W++  EFIPERF  + IDY
Sbjct: 368 PPAPLLFARESYTSINVENYIIPPYTSVMINIWHIQRDPKLWDKAEEFIPERFMNSGIDY 427

Query: 450 KGHYFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDWELPDGC---VLDAEEENGIT 509
           K H +EFIPFGSGRR C G+ FG+ ++EF +ANLLYWFDW+         LD  E+    
Sbjct: 428 KSHDYEFIPFGSGRRGCPGMSFGVAAVEFAVANLLYWFDWKFVGDTTPETLDMTEDYCFA 487

Query: 510 VCKKIPLHLKPM 519
           + KK PLH  P+
Sbjct: 488 LFKKKPLHFIPI 489

BLAST of MS027821 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 2.7e-118
Identity = 221/498 (44.38%), Postives = 318/498 (63.86%), Query Frame = 0

Query: 30  SLFLFFFFFFLVLRKFFRAKNTKNFIPSPPKLPIIGNLHQLGTLPHQSLAALSNKYGPLM 89
           ++FL   + +    K    K+T+   PSPPKLPIIGNLHQ+G+ PH+SL ALS ++GPLM
Sbjct: 27  AIFLVLLYTWYSSTKTTTQKSTQP--PSPPKLPIIGNLHQIGSYPHRSLQALSQRHGPLM 86

Query: 90  LLKLGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYDCQDMAFAPYGEFLRQA 149
           LL  G  P LVVSSAE ARE++KTHD+ F +R ++T  + LLY+ +D+A APYGE+ RQ 
Sbjct: 87  LLHFGSVPVLVVSSAEAAREILKTHDLTFSDRPKSTIFEKLLYNYKDVASAPYGEYWRQV 146

Query: 150 KKMCVLELLSAKRVESFQYVRDEEVEVLINRIREGCVGGEEAVNLKQLFASISNNIISRC 209
           + +CVL LLS +RV SF+ VR+EE + +I  I+         +NL ++F  ++N+++ + 
Sbjct: 147 RSICVLNLLSNRRVRSFRSVREEETKSMIRNIKG---SSSSVLNLSEMFVRLTNDVVCKV 206

Query: 210 VFGEKFED-ENGKS--EFRDASRRIQVLISTFCVADFFPAFGWVDVLRGFIGELKKISKK 269
             G K+ D E G+S   F++       L+ T  + D+ P   W+  + G   +L K++K+
Sbjct: 207 ALGRKYSDGEGGESGRMFKEILGEFGDLLGTVNIGDYVPWLSWLSHVNGLGAKLDKVAKQ 266

Query: 270 MDEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDTLNYHFTRDELKAILQDMFI 329
           +D+F D +++EH    S+SG     +KDF+DILL +Q++ +         +K I+ DMF 
Sbjct: 267 LDDFIDTVVQEHMNHSSRSGDDD--QKDFLDILLAIQKETSAGIPIDGVSVKGIILDMFA 326

Query: 330 AGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKIIG-KNPKFEMEDTGKMEYLHCVVKES 389
           AG+DTT +A+EW MTEL+R+   M K+Q EVR I+G +      +D  +M YL  V KE+
Sbjct: 327 AGTDTTYSALEWAMTELLRHPRVMNKLQNEVRGIVGNRTDVITEDDLVEMHYLKAVTKET 386

Query: 390 LRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQIWERPHEFIPERFTEN 449
           LRLHPP PLLVPR +  DV++ GY+I + T VF++ W I RDP+++++P EF PERF  N
Sbjct: 387 LRLHPPIPLLVPRMSTRDVEVNGYNIKANTQVFISAWQIGRDPKLYDKPEEFEPERFLNN 446

Query: 450 SIDYKGHYFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDWELPD---GCVLDAEEE 509
            IDYKG+ FE IPFG+GRR C GI F +   E  LAN+++ FDW LPD   G  LD  E 
Sbjct: 447 GIDYKGNDFELIPFGAGRRVCPGIQFAMAVNEIALANIVHKFDWALPDEASGEDLDMTET 506

Query: 510 NGITVCKKIPLHLKPMPY 521
            G+T  KK PL     P+
Sbjct: 507 TGLTAHKKYPLKAVAFPH 517

BLAST of MS027821 vs. ExPASy Swiss-Prot
Match: O81970 (Cytochrome P450 71A9 OS=Glycine max OX=3847 GN=CYP71A9 PE=2 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.1e-116
Identity = 239/506 (47.23%), Postives = 317/506 (62.65%), Query Frame = 0

Query: 26  LISFSLFLFF-FFFFLVLRKFFR---AKNTKNFIPSPPKLPIIGNLHQLGTLPHQSLAAL 85
           +ISF++F+F    F L L K  R   A+  +   P P KLP IGNLHQLGTLPHQSL  L
Sbjct: 1   MISFTVFVFLTLLFTLSLVKQLRKPTAEKRRLLPPGPRKLPFIGNLHQLGTLPHQSLQYL 60

Query: 86  SNKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYDCQDMAFAP 145
           SNK+GPLM L+LG  PTLVVSSAEMARE+ K HD  F  R    AA  L Y    ++FAP
Sbjct: 61  SNKHGPLMFLQLGSIPTLVVSSAEMAREIFKNHDSVFSGRPSLYAANRLGYG-STVSFAP 120

Query: 146 YGEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVLINRIREGCVGGEEAVNLKQLFASI 205
           YGE+ R+ +K+ +LELLS KRV+SF+ VR EEV++L+  I          VNL +L  S+
Sbjct: 121 YGEYWREMRKIMILELLSPKRVQSFEAVRFEEVKLLLQTI----ALSHGPVNLSELTLSL 180

Query: 206 SNNIISRCVFGEK----FEDENGKSEFRDASRRIQVLISTFCVADFFPAFGWVDVLRGFI 265
           +NNI+ R   G++     +D N  SE     +  Q ++  F   DFFP  GW++   G  
Sbjct: 181 TNNIVCRIALGKRNRSGADDANKVSEM---LKETQAMLGGFFPVDFFPRLGWLNKFSGLE 240

Query: 266 GELKKISKKMDEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDTLNYHFTRDEL 325
             L+KI ++MD F+D++I+EH    S S +S    +D VD+LL++Q+D       T D++
Sbjct: 241 NRLEKIFREMDNFYDQVIKEHIADNS-SERSGAEHEDVVDVLLRVQKDPNQAIAITDDQI 300

Query: 326 KAILQDMFIAGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKIIGKNPKFEMEDTGKMEY 385
           K +L D+F+AG+DT +  + W M+EL+RN  AMK+ QEEVR ++      E  D  K+ Y
Sbjct: 301 KGVLVDIFVAGTDTASATIIWIMSELIRNPKAMKRAQEEVRDLVTGKEMVEEIDLSKLLY 360

Query: 386 LHCVVKESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQIWERPHEF 445
           +  VVKE LRLHPPAPLLVPR+   +  I G+ IP+KT V VN  +I  DP  WE P+EF
Sbjct: 361 IKSVVKEVLRLHPPAPLLVPREITENCTIKGFEIPAKTRVLVNAKSIAMDPCCWENPNEF 420

Query: 446 IPERFTENSIDYKGHYFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDWELPDGC-- 505
           +PERF  + ID+KG +FE +PFG GRR C G+ F +  +E  LANLL+ FDWELP G   
Sbjct: 421 LPERFLVSPIDFKGQHFEMLPFGVGRRGCPGVNFAMPVVELALANLLFRFDWELPLGLGI 480

Query: 506 -VLDAEEENGITVCKKIPLHLKPMPY 521
             LD EE  GIT+ KK  L LK  P+
Sbjct: 481 QDLDMEEAIGITIHKKAHLWLKATPF 497

BLAST of MS027821 vs. ExPASy TrEMBL
Match: A0A6J1C5L5 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008617 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 1.1e-202
Identity = 355/521 (68.14%), Postives = 410/521 (78.69%), Query Frame = 0

Query: 1   MDQLSKFQQHWRQLQAPYGGTFHFNLISFSLFLFFFFFFLVLRKFFRAK-NTKNFIPSPP 60
           MD +S F Q W+QL        HF+LI FSL L   FFFLVL K F+ K NTKNF PSPP
Sbjct: 1   MDLISNFLQFWQQL--------HFDLIPFSLCL--IFFFLVLLKLFKPKNNTKNFPPSPP 60

Query: 61  KLPIIGNLHQLGTLPHQSLAALSNKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFC 120
           KLPIIGNLHQLG+LPHQSLAALS+KYGPLMLLKLG A TLVVSSA+MARE+MKTHD+KF 
Sbjct: 61  KLPIIGNLHQLGSLPHQSLAALSDKYGPLMLLKLGHAQTLVVSSAKMARELMKTHDLKFS 120

Query: 121 NRVRTTAAKILLYDCQDMAFAPYGEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVLIN 180
           NR +T  AK LLY  +D+ FAPYGE+ RQA+K+C LEL SAKRVESFQYVRDEEV VL+N
Sbjct: 121 NRPQTMPAKTLLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVSVLVN 180

Query: 181 RIREGCV--GGEEAVNLKQLFASISNNIISRCVFGEKFEDENGKSEFRDASRRIQVLIST 240
           RIR+GCV   GE +VNL QLF S SNNI+SRC+ GEKFEDENGKS F D SRR+ VLI+ 
Sbjct: 181 RIRKGCVDGDGEGSVNLSQLFLSTSNNIVSRCILGEKFEDENGKSRFGDTSRRVMVLITA 240

Query: 241 FCVADFFPAFGWVDVLRGFIGELKKISKKMDEFFDEIIEEHKTKMSKSGQSSYGKKDFVD 300
           FC ADFFPA  W+DV+RGF GELKK    +D F  +++EEHK KM   G+S   KKDFV 
Sbjct: 241 FCFADFFPALWWIDVVRGFNGELKKWFTTLDAFLSKVVEEHKAKMRAGGESDESKKDFVG 300

Query: 301 ILLQLQQDDTLNYHFTRDELKAILQDMFIAGSDTTATAMEWTMTELMRNQAAMKKVQEEV 360
           I+LQL Q D ++YHF+ + LK I+ DMF+ G+DTTAT +EWTMTELMRN   M+KVQEEV
Sbjct: 301 IMLQLMQSDMVDYHFSLENLKGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEV 360

Query: 361 RKIIGKNPKFEMEDTGKMEYLHCVVKESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNV 420
           RK +GK  K E+ED  KM Y++CV+KESLRLHPP PLLVPR+ + DV+I GYHIPSKT V
Sbjct: 361 RKTVGKKAKIEIEDIRKMHYMNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRV 420

Query: 421 FVNVWAIQRDPQIWERPHEFIPERFTENSIDYKGHYFEFIPFGSGRRKCSGILFGITSIE 480
           FVN W I RDP IWERP+EFIPERF ENS+DYKG  FEFIPFGSGRR C G+ FGI S E
Sbjct: 421 FVNAWKIHRDPTIWERPNEFIPERFMENSVDYKGQDFEFIPFGSGRRICPGMSFGIASFE 480

Query: 481 FVLANLLYWFDWELPDGCVLDAEEENGITVCKKIPLHLKPM 519
           + LANLLYWFDW+LP+G  LD EEENG+TV KK PLHL P+
Sbjct: 481 YALANLLYWFDWKLPNGFELDVEEENGLTVRKKNPLHLNPI 511

BLAST of MS027821 vs. ExPASy TrEMBL
Match: A0A6J1E7U9 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111431445 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 3.1e-202
Identity = 359/523 (68.64%), Postives = 413/523 (78.97%), Query Frame = 0

Query: 1   MDQLSKFQQHWRQLQAPYGGTFHFNLISFSLFLFFFFFFLVLRKFFRAKNTKNFIPSPPK 60
           MD+ S F QHW++ Q  YG   H N IS S  LFFFF  L+L+ F   K + NF PSPPK
Sbjct: 1   MDRTSYF-QHWKRQQFQYGVAIHINPISISFCLFFFFLLLLLKLFRGKKQSINFPPSPPK 60

Query: 61  LPIIGNLHQLGTLPHQSLAALSNKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFCN 120
           LPIIGNLHQLGTLPHQSLA+LS KYGPLMLLKLGQ PTLVVSSA+MAREVM+THD+KF N
Sbjct: 61  LPIIGNLHQLGTLPHQSLASLSKKYGPLMLLKLGQTPTLVVSSAKMAREVMRTHDLKFSN 120

Query: 121 RVRTTAAKILLYDCQDMAFAPYGEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVLINR 180
           R +TTA  +L Y CQDM FAPYG + RQA+KMCVLEL S KRVESFQY+RDEEV VLI+R
Sbjct: 121 RPKTTATNLLFYGCQDMGFAPYGAYWRQARKMCVLELFSFKRVESFQYIRDEEVGVLISR 180

Query: 181 IREGCVGGEEAVNLKQLFASISNNIISRCVFGEKFEDENGKSEFRDASRRIQVLISTFCV 240
           I + C GG E VNL +LF  ISNNI+SRCV G+KFEDENG + FRD SRR+  L+S FCV
Sbjct: 181 IHKACAGG-EVVNLSKLFLQISNNIVSRCVMGKKFEDENGNNRFRDVSRRVIELLSAFCV 240

Query: 241 ADFFPAFGWVDVLRGFIGELKKISKKMDEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILL 300
           ADFFPAFGWVDV+RGFIGELK IS+ MDEF D +IEEHKT++ ++G+    K+DF+DI+L
Sbjct: 241 ADFFPAFGWVDVVRGFIGELKTISRTMDEFLDMVIEEHKTEL-RTGRPD-DKRDFLDIML 300

Query: 301 QLQQDDTLNYHFTRDELKAILQDMFIAGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKI 360
           QL QDD L YHFTRD LKAILQDMF  GSDT+ATA+EW M EL+RN   +KKVQEE+R I
Sbjct: 301 QLNQDDMLGYHFTRDNLKAILQDMFAGGSDTSATALEWAMAELIRNPNTLKKVQEEIRTI 360

Query: 361 IGKNPKFEMEDTGKMEYLHCVVKESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVN 420
           +GK PK EM D  KMEY+ CV+KESLRLHPP PLLVPR+T   V I GYH+ S T+VFVN
Sbjct: 361 VGKKPKIEMNDISKMEYMKCVIKESLRLHPPIPLLVPRETSDVVDIEGYHVASGTSVFVN 420

Query: 421 VWAIQRDPQIWERPHEFIPERFTE-NSIDYKGHYFEFIPFGSGRRKCSGILFGITSIEFV 480
            WAIQRDP IWERP+EFIPERF E N  D+KG  FEFIPFGSGRRKC G+ F + S E V
Sbjct: 421 AWAIQRDPIIWERPNEFIPERFMEKNCADFKGLDFEFIPFGSGRRKCPGLSFALASFECV 480

Query: 481 LANLLYWFDWELPD---GCVLDAEEENGITVCKKIPLHLKPMP 520
           LANLLYWFDW+LP+   G +LD  E +GITV KKIPLHL P+P
Sbjct: 481 LANLLYWFDWKLPEDMAGELLDMSELHGITVRKKIPLHLTPLP 519

BLAST of MS027821 vs. ExPASy TrEMBL
Match: A0A6J1C631 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008616 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 1.2e-201
Identity = 360/527 (68.31%), Postives = 416/527 (78.94%), Query Frame = 0

Query: 1   MDQLSKFQQHWRQLQAPYGGTFHFNLISFSLFLFFFFFFLVLRKFFRAK-NTKNFIPSPP 60
           MD +SKF+Q W+Q          F+LI FSL +  FFFFLVL K  + K NTKNF PSPP
Sbjct: 1   MDPISKFEQIWQQ----------FDLIPFSLCI--FFFFLVLLKLLKPKNNTKNFPPSPP 60

Query: 61  KLPIIGNLHQLGTLPHQSLAALSNKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFC 120
           KLPIIGNLHQLG+LPHQSLAALS+K+GPLM LKLGQAPTLVVSSAEMAREVMKTHD+ F 
Sbjct: 61  KLPIIGNLHQLGSLPHQSLAALSDKHGPLMFLKLGQAPTLVVSSAEMAREVMKTHDLNFS 120

Query: 121 NRVRTTAAKILLYDCQDMAFAPYGEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVLIN 180
           +R +TTAAKILLY  +D+ FAPYGE+ RQA+K+C LEL SAKRVESFQYVRDEEV VL+N
Sbjct: 121 DRPQTTAAKILLYGFKDVGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVN 180

Query: 181 RIREGCVG--GEEAVNLKQLFASISNNIISRCVFGEKFEDENGKSEFRDASRRIQVLIST 240
           RIR GCVG  GE +VNL QLF S SNNI+SRC+ G+KFEDENGKS F D SRR+ VL++ 
Sbjct: 181 RIRRGCVGGDGEGSVNLAQLFLSTSNNIVSRCMMGDKFEDENGKSRFGDISRRVMVLLTA 240

Query: 241 FCVADFFPAFGWVDVLRGFIGELKKISKKMDEFFDEIIEEHKTKMSKSGQ-SSYGKKDFV 300
           FC ADFFPA  W+DV+RGF GELKK    +D FF +++EEHK KM   G+     K+DF+
Sbjct: 241 FCFADFFPALWWIDVVRGFNGELKKCFTTLDAFFSKVVEEHKAKMIGGGELCDESKRDFM 300

Query: 301 DILLQL-QQDDTLNYHFTRDELKAILQDMFIAGSDTTATAMEWTMTELMRNQAAMKKVQE 360
           D +LQL  QDD ++Y F+ D LKAI+ DMF+ GSDTTAT +EWTMTELMRN + MKKVQE
Sbjct: 301 DSMLQLILQDDMVDYRFSLDNLKAIVLDMFVGGSDTTATGLEWTMTELMRNPSVMKKVQE 360

Query: 361 EVRKIIGKNPKFEMEDTGKMEYLHCVVKESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKT 420
           EVRKI+G   K EMED   M Y++CV+KESLRLHPP PLLVPR+ +GDV+I GYHIPSKT
Sbjct: 361 EVRKIVGNKEKIEMEDIQNMPYMNCVIKESLRLHPPVPLLVPREAIGDVEIEGYHIPSKT 420

Query: 421 NVFVNVWAIQRDPQIWERPHEFIPERFTENSIDYKGHYFEFIPFGSGRRKCSGILFGITS 480
            VFVN W I RDP IWERP+EFIPERF ENSIDYKG  F+FIPFGSGRR C G  FGI S
Sbjct: 421 RVFVNAWKIHRDPNIWERPNEFIPERFMENSIDYKGQDFDFIPFGSGRRICPGKSFGIAS 480

Query: 481 IEFVLANLLYWFDWELPDGC-VLDAEEENGITVCKKIPLHLKPMPYL 522
            E+ LANLLYWFDW+LP G   LD EEENG+TV KKIPLHL P+ Y+
Sbjct: 481 FEYALANLLYWFDWKLPHGSKELDVEEENGLTVRKKIPLHLNPISYI 515

BLAST of MS027821 vs. ExPASy TrEMBL
Match: A0A6J1C752 (cytochrome P450 71A1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008613 PE=3 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 6.0e-193
Identity = 340/505 (67.33%), Postives = 400/505 (79.21%), Query Frame = 0

Query: 24  FNLISFSLFLFFFFFFLVLRKFFRAK-NTKNFIPSPPKLPIIGNLHQLGTLPHQSLAALS 83
           F+LI  SL L   FFFLVL K F+ K NTKN  PSPPKLPIIGNLHQLG+LPHQSLAALS
Sbjct: 7   FDLIPLSLCL--IFFFLVLLKLFKPKNNTKNLPPSPPKLPIIGNLHQLGSLPHQSLAALS 66

Query: 84  NKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYDCQDMAFAPY 143
           +K+GPLMLLKLGQ PTLVVSSAEMAREVMKTHD+ F +R  +TAA ILLY   D+AFAPY
Sbjct: 67  DKHGPLMLLKLGQVPTLVVSSAEMAREVMKTHDLNFSSRPLSTAANILLYQSHDVAFAPY 126

Query: 144 GEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVLINRIREGCVGGEEAVNLKQLFASIS 203
           G++ RQA+K+C LEL S KRVESFQYVRDEEV VL++RIR GC  GE +VNL +LF S S
Sbjct: 127 GDYWRQARKICALELFSVKRVESFQYVRDEEVAVLVDRIRRGC-DGEGSVNLCELFLSTS 186

Query: 204 NNIISRCVFGEKFEDENGKSEFRDASRRIQVLISTFCVADFFPAFGWVDVLRGFIGELKK 263
           NNI+SRC+ G+KFEDENGKS F D SRR  V+I+ FC ADF PA  W+DV+RGF GELKK
Sbjct: 187 NNIVSRCMMGDKFEDENGKSRFGDISRRAMVIITAFCFADFLPALWWIDVVRGFDGELKK 246

Query: 264 ISKKMDEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDTLNYHFTRDELKAILQ 323
               +D F  +++EEH  KM   G+S   KKDF+DI+LQL Q + ++YHF+ D LKAI+ 
Sbjct: 247 CFTTLDAFLSKVVEEHMAKMRAGGESDESKKDFMDIMLQLMQGEKVDYHFSLDHLKAIVM 306

Query: 324 DMFIAGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKIIGKNPKFEMEDTGKMEYLHCVV 383
           DMF+ GSDTTAT +EWTMTELMRN+AAM KVQEEVR+++GK  + E+ED  KMEY+ CV+
Sbjct: 307 DMFVGGSDTTATGLEWTMTELMRNRAAMDKVQEEVRRVVGKKARIEVEDIEKMEYMKCVI 366

Query: 384 KESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQIWERPHEFIPERF 443
           KESLRLHPP PLL+PR+T+GDV+I GYHIPSK  VFVNVWAIQRDP+IWERP+EFIPERF
Sbjct: 367 KESLRLHPPLPLLIPRETMGDVEIEGYHIPSKARVFVNVWAIQRDPKIWERPNEFIPERF 426

Query: 444 TE-NSIDYKGH------YFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDWELPDGC 503
            E NS   KG        +EFI FG GRRKC G+ FG+ S E  LANLL+WFDW+LP+G 
Sbjct: 427 MEKNSGGQKGRDEYGRDEYEFISFGGGRRKCPGMSFGLASFECALANLLHWFDWKLPNGS 486

Query: 504 VLDAEEENGITVCKKIPLHLKPMPY 521
            LD EEENG+TV KKIPLHL P+PY
Sbjct: 487 ELDVEEENGLTVRKKIPLHLNPIPY 508

BLAST of MS027821 vs. ExPASy TrEMBL
Match: A0A6J1C977 (cytochrome P450 71A1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008613 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.9e-191
Identity = 340/505 (67.33%), Postives = 399/505 (79.01%), Query Frame = 0

Query: 24  FNLISFSLFLFFFFFFLVLRKFFRAK-NTKNFIPSPPKLPIIGNLHQLGTLPHQSLAALS 83
           F+LI  SL L   FFFLVL K F+ K NTKN  PSPPKLPIIGNLHQLG+LPHQSLAALS
Sbjct: 7   FDLIPLSLCL--IFFFLVLLKLFKPKNNTKNLPPSPPKLPIIGNLHQLGSLPHQSLAALS 66

Query: 84  NKYGPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYDCQDMAFAPY 143
           +K+GPLMLLKLGQ PTLVVSSAEMAREVMKTHD+ F +R  +TAA ILLY   D+AFAPY
Sbjct: 67  DKHGPLMLLKLGQVPTLVVSSAEMAREVMKTHDLNFSSRPLSTAANILLYQSHDVAFAPY 126

Query: 144 GEFLRQAKKMCVLELLSAKRVESFQYVRDEEVEVLINRIREGCVGGEEAVNLKQLFASIS 203
           G++ RQA+K+C LEL S KRVESFQYVRDEEV VL++RIR GC  GE +VNL +LF S S
Sbjct: 127 GDYWRQARKICALELFSVKRVESFQYVRDEEVAVLVDRIRRGC-DGEGSVNLCELFLSTS 186

Query: 204 NNIISRCVFGEKFEDENGKSEFRDASRRIQVLISTFCVADFFPAFGWVDVLRGFIGELKK 263
           NNI+SRC+ G+KFEDENGKS F D SRR  V+I+ FC ADF PA  W+DV+RGF GELKK
Sbjct: 187 NNIVSRCMMGDKFEDENGKSRFGDISRRAMVIITAFCFADFLPALWWIDVVRGFDGELKK 246

Query: 264 ISKKMDEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDTLNYHFTRDELKAILQ 323
               +D F  +++EEH  KM   G+S   KKDF+DI+LQL Q + ++YHF+ D LKAI  
Sbjct: 247 CFTTLDAFLSKVVEEHMAKMRAGGESDESKKDFMDIMLQLMQGEKVDYHFSLDHLKAI-- 306

Query: 324 DMFIAGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKIIGKNPKFEMEDTGKMEYLHCVV 383
           DMF+ GSDTTAT +EWTMTELMRN+AAM KVQEEVR+++GK  + E+ED  KMEY+ CV+
Sbjct: 307 DMFVGGSDTTATGLEWTMTELMRNRAAMDKVQEEVRRVVGKKARIEVEDIEKMEYMKCVI 366

Query: 384 KESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQIWERPHEFIPERF 443
           KESLRLHPP PLL+PR+T+GDV+I GYHIPSK  VFVNVWAIQRDP+IWERP+EFIPERF
Sbjct: 367 KESLRLHPPLPLLIPRETMGDVEIEGYHIPSKARVFVNVWAIQRDPKIWERPNEFIPERF 426

Query: 444 TE-NSIDYKGH------YFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDWELPDGC 503
            E NS   KG        +EFI FG GRRKC G+ FG+ S E  LANLL+WFDW+LP+G 
Sbjct: 427 MEKNSGGQKGRDEYGRDEYEFISFGGGRRKCPGMSFGLASFECALANLLHWFDWKLPNGS 486

Query: 504 VLDAEEENGITVCKKIPLHLKPMPY 521
            LD EEENG+TV KKIPLHL P+PY
Sbjct: 487 ELDVEEENGLTVRKKIPLHLNPIPY 506

BLAST of MS027821 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 418.7 bits (1075), Expect = 6.9e-117
Identity = 219/499 (43.89%), Postives = 313/499 (62.73%), Query Frame = 0

Query: 33  LFFFFFFLVLRKFFRAKNTKNF---IPSPPKLPIIGNLHQLGTLPHQSLAALSNKYGPLM 92
           L   F   +L   F  KN +N+    PSPP  PIIGNLHQLG LPHQSL  LS KYGP+M
Sbjct: 7   LSLIFVICILVAVFNHKNRRNYQRTPPSPPGCPIIGNLHQLGELPHQSLWKLSKKYGPVM 66

Query: 93  LLKLGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYDCQDMAFAPYGEFLRQA 152
           LLKLG+ PT++VSS+E A++ +K HD+  C+R     A+ L Y+  D+AF+PY ++ ++ 
Sbjct: 67  LLKLGRVPTVIVSSSETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPYDDYWKEV 126

Query: 153 KKMCVLELLSAKRVESFQYVRDEEVEVLINRIREGCVGGEEAVNLKQLFASISNNIISRC 212
           +K+ V EL S+K+V S Q ++DEEV+ LI+ I E     +  +NL +   +++ +++ R 
Sbjct: 127 RKLAVQELFSSKQVHSIQPIKDEEVKKLIDSISES-AAQKTPINLNKTLLALTVSVVCRT 186

Query: 213 VFGEKFEDENGKSE-FRDASRRIQVLISTFCVADFFPAFG-WVDVLRGFIGELKKISKKM 272
            F   FE     SE F +  R    ++ +F  +DF P  G  +D+L G  G  ++  + +
Sbjct: 187 AFSVNFEGTVLNSERFNNIVREALEMLGSFSASDFIPYVGRIIDLLTGLQGRRERSMRDL 246

Query: 273 DEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDTL--NYHFTRDELKAILQDMF 332
           D F++++ + HK K  +      G +DFVD+LL+L++++ +  N   TR+ +KAIL D+ 
Sbjct: 247 DAFYEQMFDLHKQKKEE------GSEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILMDVL 306

Query: 333 IAGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKIIGKNPKFEMEDTGKMEYLHCVVKES 392
           +AG DT+A  M W M EL +N   MKKVQ E+R  I    +   +DT K+EYL  V+KE+
Sbjct: 307 LAGMDTSAITMTWAMAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVIKET 366

Query: 393 LRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQIWERPHEFIPERFTEN 452
            RLHP  PLL+PR+ + + +I GY IP KT + VNVWAI RDP  W+ P  F+PERFT+N
Sbjct: 367 WRLHPTTPLLIPREAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFTDN 426

Query: 453 SIDYKGHYFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDWELPDGCV---LDAEEE 512
           +ID KG +FE +PFG GRR C  +  G T +EF LANLLY FDW+LP+G     +D EE 
Sbjct: 427 NIDAKGQHFELLPFGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDMEEA 486

Query: 513 NGITVCKKIPLHLKPMPYL 522
            G+TV KK  L L P  +L
Sbjct: 487 PGLTVNKKNELILVPTKFL 498

BLAST of MS027821 vs. TAIR 10
Match: AT3G26290.1 (cytochrome P450, family 71, subfamily B, polypeptide 26 )

HSP 1 Score: 416.0 bits (1068), Expect = 4.5e-116
Identity = 218/499 (43.69%), Postives = 313/499 (62.73%), Query Frame = 0

Query: 31  LFLFFFFFFLVLRKFFRAKNTKN-FIPSPPKLPIIGNLHQLGTLPHQSLAALSNKYGPLM 90
           L L FF  FL+L  F R  + K+  IPSPP  PIIGNLHQLG L HQSL  LS KYGP+M
Sbjct: 7   LSLLFFIIFLLLAAFKRKNHGKHRRIPSPPGFPIIGNLHQLGELQHQSLWKLSKKYGPVM 66

Query: 91  LLKLGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYDCQDMAFAPYGEFLRQA 150
           LLKLG+ PTL++SS+E A++ ++ +D+  C+R      + L Y+  DM+ +PY E+ ++ 
Sbjct: 67  LLKLGKVPTLILSSSETAKQALRDYDLHCCSRPSLAGGRELSYNNLDMSSSPYNEYWKEL 126

Query: 151 KKMCVLELLSAKRVESFQYVRDEEVEVLINRIREGCVGGEEAVNLKQLFASISNNIISRC 210
           +K+C  EL SA +++S Q ++DEEV+ +I+ I E     +  VNL + F +++ +++ + 
Sbjct: 127 RKLCSQELFSANKIQSIQPIKDEEVKKVIDSIAESS-SLKNPVNLSKTFLALTTSVVCKA 186

Query: 211 VFGEKFEDENGKSE-FRDASRRIQVLISTFCVADFFPAFGW-VDVLRGFIGELKKISKKM 270
            FG  FE     S+ F    R    ++ +F  +DF P  GW +D   G  G  KK  + +
Sbjct: 187 AFGVSFEGSVLNSDRFNKLVRDTFEMLGSFSASDFIPYVGWIIDKFNGLQGWRKKSFRDL 246

Query: 271 DEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDTL--NYHFTRDELKAILQDMF 330
           D F+++I + HK       +   G +D VD+LL+L++++ +  N   TR+ +KAIL ++ 
Sbjct: 247 DAFYEQIFDLHKE------EKEVGSEDLVDVLLRLEKEEIVVGNGKLTRNHIKAILMNIL 306

Query: 331 IAGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKIIGKNPKFEMEDTGKMEYLHCVVKES 390
           + G DT+A  M W M EL +N   MKKVQ E+R  I    +   +DT K+EYL  V+KE+
Sbjct: 307 LGGIDTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNKERISFDDTDKLEYLKMVIKET 366

Query: 391 LRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQIWERPHEFIPERFTEN 450
            RLHPP PLL+PR  + + +I GY IP+KT + VNVWAI RDP  W+ P  F+PERF ++
Sbjct: 367 WRLHPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDPEMFLPERFNDS 426

Query: 451 SIDYKGHYFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDWELPDGCV---LDAEEE 510
           +ID KG  FE + FGSGRR C G+  G T +EF LAN+LY FDW+LP+G V   +D EE 
Sbjct: 427 NIDAKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEGMVVEDIDMEEA 486

Query: 511 NGITVCKKIPLHLKPMPYL 522
            G+TV KK  L L P+ YL
Sbjct: 487 PGLTVSKKSELVLVPVKYL 498

BLAST of MS027821 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 410.6 bits (1054), Expect = 1.9e-114
Identity = 209/486 (43.00%), Postives = 306/486 (62.96%), Query Frame = 0

Query: 33  LFFFFFFLVLRKFFRAKNTKNFIPSPPKLPIIGNLHQLGTLPHQSLAALSNKYGPLMLLK 92
           + F    ++ RK  R K  +N +PSPP LP+IGNLHQLG  PH+SL +LS++YGPLMLL 
Sbjct: 11  IIFVVTIIIFRKQKRGKK-RNTLPSPPGLPLIGNLHQLGRHPHRSLCSLSHRYGPLMLLH 70

Query: 93  LGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYDCQDMAFAPYGEFLRQAKKM 152
            G+ P LVVSSAE+AR+V+KTHD  F +R R+   + LLYD  D+A APYGE+ RQ K +
Sbjct: 71  FGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPYGEYWRQMKSV 130

Query: 153 CVLELLSAKRVESFQYVRDEEVEVLINRIREGCVGGEEAVNLKQLFASISNNIISRCVFG 212
           CVL L S K V SF+ VR+EE+ +++ +IR+        VNL ++  S++N++I +   G
Sbjct: 131 CVLHLFSNKMVRSFREVREEEISLMMEKIRKSI---SLPVNLSKILVSLTNDVICKVALG 190

Query: 213 EKFEDENGKSEFRDASRRIQVLISTFCVADFFPAFGWVDVLRGFIGELKKISKKMDEFFD 272
            K+    G+++F++   R+  L+ TF V  + P   W+D +RG   +L+K +  +D+FF+
Sbjct: 191 RKY---GGETDFKELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTANDVDKFFE 250

Query: 273 EIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDTLNYHFTRDELKAILQDMFIAGSDTT 332
            ++++H          +    DFVD+LL +Q+D T+ +   R  +KAI+ ++F+ G+DT+
Sbjct: 251 RVVQDHV-------DGNRDMTDFVDVLLAIQRDKTVGFEINRVSIKAIVMNVFVGGTDTS 310

Query: 333 ATAMEWTMTELMRNQAAMKKVQEEVRKIIGKNPKFEMEDTGKMEYLHCVVKESLRLHPPA 392
           +T MEW MTEL+R+   +K++QEEVR I         E+   M YL  V+KE+LRLHPP 
Sbjct: 311 STLMEWAMTELLRHPKCLKRLQEEVRTICKDKSSVSEEEIQNMSYLKAVIKEALRLHPPL 370

Query: 393 PLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQIW-ERPHEFIPERFTENSIDYKG 452
           PL+VP ++  DV++G +HIP+ T V +N WAI R+   W     EF PER  ++S+DY+G
Sbjct: 371 PLMVPHESTQDVRLGDHHIPAGTQVLINAWAIGREAATWGPDVEEFRPERHLDSSVDYRG 430

Query: 453 HYFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDWELPDGCVLD---AEEENGITVC 512
             FE IPFGSGRR C  I F +   E VLANL++ FDW L      D     E  GI + 
Sbjct: 431 QAFELIPFGSGRRICPAISFAVVLNEVVLANLVHRFDWRLSVESTEDQTEVAESTGIAIH 482

Query: 513 KKIPLH 515
           +  PL+
Sbjct: 491 RMFPLY 482

BLAST of MS027821 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 409.5 bits (1051), Expect = 4.2e-114
Identity = 203/493 (41.18%), Postives = 309/493 (62.68%), Query Frame = 0

Query: 26  LISFSLFLFFFFFFLVLRKFFRAKNTKNFIPSPPKLPIIGNLHQLGTLPHQSLAALSNKY 85
           +I  SL +F    F + +K  +  NT     SPP+LP+IGNLHQLG  PH+SL +LSN+Y
Sbjct: 7   IILLSLIIFITILFFIKQKKGKKSNTP---ASPPRLPLIGNLHQLGRHPHRSLCSLSNRY 66

Query: 86  GPLMLLKLGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYDCQDMAFAPYGEF 145
           GPLMLL+ G  P LVVSSA++AR+++KT+D  F +R R+   + + Y+ +D+A APYGE+
Sbjct: 67  GPLMLLRFGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPYGEY 126

Query: 146 LRQAKKMCVLELLSAKRVESFQYVRDEEVEVLINRIREGCVGGEEAVNLKQLFASISNNI 205
            RQ K +CVL LL+ K V SF+ VR EE+ +++ +I++        VNL +L  S++N++
Sbjct: 127 WRQMKSVCVLHLLTNKMVRSFRNVRQEEISLMMEKIQK---SSSLQVNLSELLGSLTNDV 186

Query: 206 ISRCVFGEKFEDENGKSEFRDASRRIQVLISTFCVADFFPAFGWVDVLRGFIGELKKISK 265
           ISR   G K+ DE   ++F++  +R+  L+  FCV  + P   W+D + G  G+LKK   
Sbjct: 187 ISRVALGRKYSDE---TDFKELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGN 246

Query: 266 KMDEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDTLNYHFTRDELKAILQDMF 325
            +DEF ++++++H+            + DFVD+LL++Q++ ++ +   R  +KAI+ D+ 
Sbjct: 247 DLDEFLEKVVQDHE-------DGDAQRTDFVDVLLRIQREKSVGFEIDRLSIKAIILDVV 306

Query: 326 IAGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKIIGKNPKFEMEDTGKMEYLHCVVKES 385
           + G+DT+   MEW MTEL+     + ++QEEVR I   N     +D   M YL  V+KE+
Sbjct: 307 VGGTDTSYALMEWAMTELLHRPECLNRLQEEVRTICKGNSSVSEDDIKDMNYLKAVIKET 366

Query: 386 LRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQIWERPHE-FIPERFTE 445
           +RLHPP PL+VP ++  DV++G YHIP+ T V +N WAI R+   W    E F PER   
Sbjct: 367 MRLHPPLPLMVPHESTQDVRLGDYHIPAGTQVMINAWAIGREAATWGPDAEKFRPERHLN 426

Query: 446 NSIDYKGHYFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDWELPDGCVLD---AEE 505
           +S+D++GH FE IPFG+GRR C  I F +  IE  LANL++ +DW LP+  + D     E
Sbjct: 427 SSVDFRGHNFELIPFGAGRRICPAISFAVILIEVTLANLVHRYDWRLPEEYIEDQTNVAE 483

Query: 506 ENGITVCKKIPLH 515
             G+ + +  PL+
Sbjct: 487 STGMVIHRLFPLY 483

BLAST of MS027821 vs. TAIR 10
Match: AT3G26320.1 (cytochrome P450, family 71, subfamily B, polypeptide 36 )

HSP 1 Score: 408.7 bits (1049), Expect = 7.1e-114
Identity = 215/501 (42.91%), Postives = 310/501 (61.88%), Query Frame = 0

Query: 31  LFLFFFFFFLVLRKFFRAKNTKNF---IPSPPKLPIIGNLHQLGTLPHQSLAALSNKYGP 90
           LFL   F   +L   F  K  +      PSPP  PIIGNLHQLG LPHQSL  LS KYG 
Sbjct: 5   LFLSLLFLSCILLAAFTHKKRQQHQRKPPSPPGFPIIGNLHQLGELPHQSLWRLSKKYGH 64

Query: 91  LMLLKLGQAPTLVVSSAEMAREVMKTHDIKFCNRVRTTAAKILLYDCQDMAFAPYGEFLR 150
           +MLLK G  PT+VVSS+E A++V+K HD+  C+R      + L Y+  D+AF+P+ ++ +
Sbjct: 65  VMLLKFGSIPTVVVSSSETAKQVLKIHDLHCCSRPSLAGPRALSYNYLDIAFSPFDDYWK 124

Query: 151 QAKKMCVLELLSAKRVESFQYVRDEEVEVLINRIREGCVGGEEAVNLKQLFASISNNIIS 210
           + +++CV EL S KRV+SFQ ++++EV+ LI+ + E    G   VNL + F S++  +  
Sbjct: 125 ELRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVSESASQG-TPVNLSEKFTSLTVRVTC 184

Query: 211 RCVFGEKFEDENGKSE-FRDASRRIQVLISTFCVADFFPAFGW-VDVLRGFIGELKKISK 270
           +  FG  F+     S+ F        + + +F  +D+FP  GW +D L G  G+ ++  +
Sbjct: 185 KATFGVNFQGTVLNSDRFEKLIHDTYLFLGSFSASDYFPNGGWIIDWLTGLHGQRERSVR 244

Query: 271 KMDEFFDEIIEEHKTKMSKSGQSSYGKKDFVDILLQLQQDDT-LNY-HFTRDELKAILQD 330
            +D F++++ + HK        +  G +DFVD+LL+L++++T + Y   TR+ +KAIL +
Sbjct: 245 ALDAFYEQMFDLHKQ------GNKEGVEDFVDLLLRLEKEETVIGYGKLTRNHIKAILMN 304

Query: 331 MFIAGSDTTATAMEWTMTELMRNQAAMKKVQEEVRKIIGKNPKFEMEDTGKMEYLHCVVK 390
           + I G  T+A  M W MTELMRN   MKKVQ E+R  IGK     ++D  ++ YL  V+ 
Sbjct: 305 VLIGGIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGKKSMITLDDIDQLHYLKMVIN 364

Query: 391 ESLRLHPPAPLLVPRQTLGDVKIGGYHIPSKTNVFVNVWAIQRDPQIWERPHEFIPERFT 450
           E+ RLHPP+P L+PRQ + + ++  Y IP KT ++VNVWAI RDP  W+ P EF+PERF 
Sbjct: 365 ETWRLHPPSPFLIPRQVMSEFELNDYVIPVKTRLYVNVWAIGRDPDTWKDPEEFLPERFV 424

Query: 451 ENSIDYKGHYFEFIPFGSGRRKCSGILFGITSIEFVLANLLYWFDWELPDGCV---LDAE 510
            +SID KG +FE +PFGSGRR C  +  G T +EF LAN+LY FDW++P G V   +D E
Sbjct: 425 NSSIDAKGQHFELLPFGSGRRMCPAMYMGTTMVEFGLANMLYHFDWKIPVGMVAEDIDLE 484

Query: 511 EENGITVCKKIPLHLKPMPYL 522
           E  G+   KK  L L P+ YL
Sbjct: 485 ESPGLNASKKNELVLVPLKYL 498

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895465.11.3e-21372.99cytochrome P450 71A1-like [Benincasa hispida][more]
XP_022137055.12.2e-20268.14cytochrome P450 71A1-like [Momordica charantia][more]
XP_022923856.16.5e-20268.64cytochrome P450 71A1-like [Cucurbita moschata][more]
XP_022137054.12.5e-20168.31cytochrome P450 71A1-like [Momordica charantia][more]
XP_023520088.13.2e-20168.45cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A068Q6093.9e-14951.23Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP... [more]
P244657.6e-14551.33Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
A0A0N9HTU15.1e-12546.95Desmethylyatein synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71CU1 PE=1 ... [more]
A0A068Q6L22.7e-11844.38Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1[more]
O819701.1e-11647.23Cytochrome P450 71A9 OS=Glycine max OX=3847 GN=CYP71A9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C5L51.1e-20268.14cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008617 PE=3 SV... [more]
A0A6J1E7U93.1e-20268.64cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111431445 PE=3 SV=... [more]
A0A6J1C6311.2e-20168.31cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008616 PE=3 SV... [more]
A0A6J1C7526.0e-19367.33cytochrome P450 71A1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
A0A6J1C9771.9e-19167.33cytochrome P450 71A1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
Match NameE-valueIdentityDescription
AT3G26300.16.9e-11743.89cytochrome P450, family 71, subfamily B, polypeptide 34 [more]
AT3G26290.14.5e-11643.69cytochrome P450, family 71, subfamily B, polypeptide 26 [more]
AT3G48270.11.9e-11443.00cytochrome P450, family 71, subfamily A, polypeptide 26 [more]
AT3G48310.14.2e-11441.18cytochrome P450, family 71, subfamily A, polypeptide 22 [more]
AT3G26320.17.1e-11442.91cytochrome P450, family 71, subfamily B, polypeptide 36 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 456..466
score: 56.72
coord: 316..333
score: 36.43
coord: 336..362
score: 27.58
coord: 379..397
score: 34.56
coord: 420..444
score: 37.87
coord: 466..489
score: 24.83
coord: 201..219
score: 28.53
coord: 106..127
score: 20.66
coord: 82..101
score: 34.61
IPR001128Cytochrome P450PRINTSPR00385P450coord: 380..391
score: 42.06
coord: 457..466
score: 57.61
coord: 327..344
score: 36.92
IPR001128Cytochrome P450PFAMPF00067p450coord: 56..507
e-value: 2.1E-106
score: 356.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 43..516
e-value: 1.0E-130
score: 438.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 55..519
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 31..519
NoneNo IPR availablePANTHERPTHR47956:SF10CYTOCHROME P450 FAMILY 71 PROTEINcoord: 31..519

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS027821.1MS027821.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen