MS027618 (gene) Bitter gourd (TR) v1

Overview


Name	MS027618
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	LOW QUALITY PROTEIN: deacetylvindoline O-acetyltransferase-like
Location	scaffold20: 1112560 .. 1112781 (+)
RNA-Seq Expression	MS027618
Synteny	MS027618

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGTCCCACAAGCTGGCCGATGCTTCCACATTTGTCAACTTCATGAACCATTGGGCTTCTGTCACCCGCGGCGGCACCCCGCCGCCTCCTCGGTTCAATGCAGCGAGCTTGTTCGGGGAAGCTGCCTGCGACAACGACAGCCTAATGGCGGTGCAAGGTTCAAAAATAGTGAAACGAGACGAGAGTATTCTGTCGAAGAGATTCGTATTTGAGGGGTAG

mRNA sequence

ATGCTGTCCCACAAGCTGGCCGATGCTTCCACATTTGTCAACTTCATGAACCATTGGGCTTCTGTCACCCGCGGCGGCACCCCGCCGCCTCCTCGGTTCAATGCAGCGAGCTTGTTCGGGGAAGCTGCCTGCGACAACGACAGCCTAATGGCGGTGCAAGGTTCAAAAATAGTGAAACGAGACGAGAGTATTCTGTCGAAGAGATTCGTATTTGAGGGGTAG

Coding sequence (CDS)

ATGCTGTCCCACAAGCTGGCCGATGCTTCCACATTTGTCAACTTCATGAACCATTGGGCTTCTGTCACCCGCGGCGGCACCCCGCCGCCTCCTCGGTTCAATGCAGCGAGCTTGTTCGGGGAAGCTGCCTGCGACAACGACAGCCTAATGGCGGTGCAAGGTTCAAAAATAGTGAAACGAGACGAGAGTATTCTGTCGAAGAGATTCGTATTTGAGGGGTAG

Protein sequence

MLSHKLADASTFVNFMNHWASVTRGGTPPPPRFNAASLFGEAACDNDSLMAVQGSKIVKRDESILSKRFVFEG

Homology

[Close]

BLAST of MS027618 vs. NCBI nr
Match: XP_022143321.1 (LOW QUALITY PROTEIN: deacetylvindoline O-acetyltransferase-like [Momordica charantia])

HSP 1 Score: 149.4 bits (376), Expect = 1.2e-32
Identity = 71/73 (97.26%), Postives = 73/73 (100.00%), Query Frame = 0


Query: 1   MLSHKLADASTFVNFMNHWASVTRGGTPPPPRFNAASLFGEAACDNDSLMAVQGSKIVKR 60
           MLSH+LADASTFVNFMNHWASVTRGGTPPPPRFNAASLFG+AACDNDSLMAVQGSKIVKR
Sbjct: 290 MLSHRLADASTFVNFMNHWASVTRGGTPPPPRFNAASLFGDAACDNDSLMAVQGSKIVKR 349

Query: 61  DESILSKRFVFEG 74
           DESILSKRFVFEG
Sbjct: 350 DESILSKRFVFEG 362

[Close]

BLAST of MS027618 vs. NCBI nr
Match: XP_031740025.1 (stemmadenine O-acetyltransferase isoform X2 [Cucumis sativus])

HSP 1 Score: 84.0 bits (206), Expect = 6.0e-13
Identity = 45/76 (59.21%), Postives = 55/76 (72.37%), Query Frame = 0


Query: 2   LSHKLADASTFVNFMNHWASVTRGGTP-----PPPRFNAASLFGEAACDNDSLMAVQGSK 61
           LSHKLADASTF NFMNHWAS++R G       PPPRF+A SLF  A  D+     V GSK
Sbjct: 150 LSHKLADASTFTNFMNHWASLSRTGDHDMPLLPPPRFDAGSLFEAATVDD-----VLGSK 209

Query: 62  IVKRDESILSKRFVFE 73
           IVKR+++++SKR VF+
Sbjct: 210 IVKREKNLVSKRMVFK 220

[Close]

BLAST of MS027618 vs. NCBI nr
Match: XP_004152371.2 (stemmadenine O-acetyltransferase isoform X1 [Cucumis sativus] >KGN52789.1 hypothetical protein Csa_015235 [Cucumis sativus])

HSP 1 Score: 83.6 bits (205), Expect = 7.9e-13
Identity = 44/76 (57.89%), Postives = 56/76 (73.68%), Query Frame = 0


Query: 2   LSHKLADASTFVNFMNHWASVTRGGTP-----PPPRFNAASLFGEAACDNDSLMAVQGSK 61
           LSHKLADASTF NFMNHWAS++R G       PPPRF+A SLF  A  D+   +  +GSK
Sbjct: 150 LSHKLADASTFTNFMNHWASLSRTGDHDMPLLPPPRFDAGSLFEAATVDD---VLGEGSK 209

Query: 62  IVKRDESILSKRFVFE 73
           IVKR+++++SKR VF+
Sbjct: 210 IVKREKNLVSKRMVFK 222

[Close]

BLAST of MS027618 vs. NCBI nr
Match: XP_023528950.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 67.4 bits (163), Expect = 5.8e-08
Identity = 37/77 (48.05%), Postives = 48/77 (62.34%), Query Frame = 0


Query: 2   LSHKLADASTFVNFMNHWASVTR--GGTPPP---PRFNAASLFGEAACDNDSLMAVQGSK 61
           L+HK+AD +TF NFMN WAS+ R  GG+ PP   P F+AASLF             +GS 
Sbjct: 156 LAHKVADLATFANFMNDWASIARSSGGSDPPVVCPLFHAASLFRPELNGGGDPSEGEGSG 215

Query: 62  IVKRDESILSKRFVFEG 74
             KR+E++ S+R VFEG
Sbjct: 216 SKKREENVRSRRMVFEG 232

[Close]

BLAST of MS027618 vs. NCBI nr
Match: XP_022935660.1 (vinorine synthase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 65.9 bits (159), Expect = 1.7e-07
Identity = 37/77 (48.05%), Postives = 47/77 (61.04%), Query Frame = 0


Query: 2   LSHKLADASTFVNFMNHWASVTR--GGTPPP---PRFNAASLFGEAACDNDSLMAVQGSK 61
           L+HK+AD +TF NFMN WAS+ R  GG  PP   P F+AASLF             +GS 
Sbjct: 156 LAHKVADLATFANFMNDWASIARSSGGIDPPVVCPLFHAASLFRPELDGGVDPSEGEGSG 215

Query: 62  IVKRDESILSKRFVFEG 74
             KR+E++ S+R VFEG
Sbjct: 216 SKKREENVRSRRMVFEG 232

[Close]

BLAST of MS027618 vs. ExPASy TrEMBL
Match: A0A6J1CPX9 (LOW QUALITY PROTEIN: deacetylvindoline O-acetyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111013222 PE=3 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 5.7e-33
Identity = 71/73 (97.26%), Postives = 73/73 (100.00%), Query Frame = 0


Query: 1   MLSHKLADASTFVNFMNHWASVTRGGTPPPPRFNAASLFGEAACDNDSLMAVQGSKIVKR 60
           MLSH+LADASTFVNFMNHWASVTRGGTPPPPRFNAASLFG+AACDNDSLMAVQGSKIVKR
Sbjct: 290 MLSHRLADASTFVNFMNHWASVTRGGTPPPPRFNAASLFGDAACDNDSLMAVQGSKIVKR 349

Query: 61  DESILSKRFVFEG 74
           DESILSKRFVFEG
Sbjct: 350 DESILSKRFVFEG 362

[Close]

BLAST of MS027618 vs. ExPASy TrEMBL
Match: A0A0A0KV47 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G001560 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.8e-13
Identity = 44/76 (57.89%), Postives = 56/76 (73.68%), Query Frame = 0


Query: 2   LSHKLADASTFVNFMNHWASVTRGGTP-----PPPRFNAASLFGEAACDNDSLMAVQGSK 61
           LSHKLADASTF NFMNHWAS++R G       PPPRF+A SLF  A  D+   +  +GSK
Sbjct: 150 LSHKLADASTFTNFMNHWASLSRTGDHDMPLLPPPRFDAGSLFEAATVDD---VLGEGSK 209

Query: 62  IVKRDESILSKRFVFE 73
           IVKR+++++SKR VF+
Sbjct: 210 IVKREKNLVSKRMVFK 222

[Close]

BLAST of MS027618 vs. ExPASy TrEMBL
Match: A0A6J1F5C1 (vinorine synthase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442451 PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 8.2e-08
Identity = 37/77 (48.05%), Postives = 47/77 (61.04%), Query Frame = 0


Query: 2   LSHKLADASTFVNFMNHWASVTR--GGTPPP---PRFNAASLFGEAACDNDSLMAVQGSK 61
           L+HK+AD +TF NFMN WAS+ R  GG  PP   P F+AASLF             +GS 
Sbjct: 156 LAHKVADLATFANFMNDWASIARSSGGIDPPVVCPLFHAASLFRPELDGGVDPSEGEGSG 215

Query: 62  IVKRDESILSKRFVFEG 74
             KR+E++ S+R VFEG
Sbjct: 216 SKKREENVRSRRMVFEG 232

[Close]

BLAST of MS027618 vs. ExPASy TrEMBL
Match: A0A6J1FBA0 (vinorine synthase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442451 PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 8.2e-08
Identity = 37/77 (48.05%), Postives = 47/77 (61.04%), Query Frame = 0


Query: 2   LSHKLADASTFVNFMNHWASVTR--GGTPPP---PRFNAASLFGEAACDNDSLMAVQGSK 61
           L+HK+AD +TF NFMN WAS+ R  GG  PP   P F+AASLF             +GS 
Sbjct: 156 LAHKVADLATFANFMNDWASIARSSGGIDPPVVCPLFHAASLFRPELDGGVDPSEGEGSG 215

Query: 62  IVKRDESILSKRFVFEG 74
             KR+E++ S+R VFEG
Sbjct: 216 SKKREENVRSRRMVFEG 232

[Close]

BLAST of MS027618 vs. ExPASy TrEMBL
Match: A0A6J1F342 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111441702 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 3.8e-05
Identity = 34/77 (44.16%), Postives = 41/77 (53.25%), Query Frame = 0


Query: 3   SHKLADASTFVNFMNHWASVTR----GGTP-PPPRFNAASLF-GEAACDNDSLMAVQGSK 62
           SHK+AD ++  NFMN WAS+ R    G  P   P  N  S F  E     D      GS 
Sbjct: 157 SHKIADLASLTNFMNDWASIARSSDDGDLPVVNPEINGVSYFPPELDAGGDPFGGGDGSG 216

Query: 63  IVKRDESILSKRFVFEG 74
            V+RDE++ SKR VFEG
Sbjct: 217 TVRRDENVCSKRLVFEG 233

[Close]

BLAST of MS027618 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 44.7 bits (104), Expect = 3.8e-05
Identity = 30/72 (41.67%), Postives = 42/72 (58.33%), Query Frame = 0


Query: 2   LSHKLADASTFVNFMNHWASVTRGGTPP--PPRFNAASLFGEAACDNDSLMAVQGSKIVK 61
           LSHKL DA + + F+N WA+  RG T     P F+ A +F    CD ++L    G  I K
Sbjct: 151 LSHKLCDALSGLIFVNSWAAFARGQTDEIITPSFDLAKMF--PPCDIENLNMATG--ITK 210

Query: 62  RDESILSKRFVF 72
             E+I+++RFVF
Sbjct: 211 --ENIVTRRFVF 216

The following BLAST results are available for this feature:

BLAST of MS027618 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143321.1	1.2e-32	97.26	LOW QUALITY PROTEIN: deacetylvindoline O-acetyltransferase-like [Momordica chara...	[more]
XP_031740025.1	6.0e-13	59.21	stemmadenine O-acetyltransferase isoform X2 [Cucumis sativus]	[more]
XP_004152371.2	7.9e-13	57.89	stemmadenine O-acetyltransferase isoform X1 [Cucumis sativus] >KGN52789.1 hypoth...	[more]
XP_023528950.1	5.8e-08	48.05	vinorine synthase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022935660.1	1.7e-07	48.05	vinorine synthase-like isoform X1 [Cucurbita moschata]	[more]

BLAST of MS027618 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS027618 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CPX9	5.7e-33	97.26	LOW QUALITY PROTEIN: deacetylvindoline O-acetyltransferase-like OS=Momordica cha...	[more]
A0A0A0KV47	3.8e-13	57.89	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G001560 PE=3 SV=1	[more]
A0A6J1F5C1	8.2e-08	48.05	vinorine synthase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442451 ...	[more]
A0A6J1FBA0	8.2e-08	48.05	vinorine synthase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442451 ...	[more]
A0A6J1F342	3.8e-05	44.16	vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111441702 PE=3 SV=1	[more]

BLAST of MS027618 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24430.1	3.8e-05	41.67	HXXXD-type acyl-transferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003480	Transferase	PFAM	PF02458	Transferase	coord: 2..55 e-value: 1.3E-5 score: 24.0
IPR023213	Chloramphenicol acetyltransferase-like domain superfamily	GENE3D	3.30.559.10		coord: 1..58 e-value: 3.9E-8 score: 34.9

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS027618.1	MS027618.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups