MS027560 (gene) Bitter gourd (TR) v1

Overview


Name	MS027560
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectinesterase
Location	scaffold313: 1775078 .. 1776950 (-)
RNA-Seq Expression	MS027560
Synteny	MS027560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGACAGCGGTGGTGTCGGTTCTATCACTTATTTTAGTGGTGGGAGTTGCTTTGGCAGTTGTGGCAGTTGTTAACAGGAACCACGGCAGTTTTGACGGCGAGAATTTGTCCCCCAAAATGAAAGCTGTGGCCACTATATGTTCTCATACTGATTACCAAGAAGAGTGTCAAAATACTCTGGGCAGCATCGCCCACAATTCCTCCTCGGACGACCCGAAGGAGTACATTAAATCAGCGATTATGGCCACTGTTGAGGAGTTTAAGAAAGGGTTCAAATTGACGGACTCCCTTATGGTGGAGGCTGGTAACAATTCCTTCATGAAAATGTCGGTTGAAGATTGCAAAGATTTGCTGCAGTTTGCCGTTGAAGAGCTTCAGGCTTCTTACTCCTCCGTCGGGGACAGCGATGAGCGCTCCCAAGAGGAGCGAGTCGCTGACATCAATACCTGGCTCAGCGCCGTCATTTCTTATCAGCAATCTTGCCTCGACGGCCTCGGAGAAGCCAACCCCAAGCTCAAAGAAACCATGGAAGGTGGTATTGACATTGCACGCAAGCTTACCAGCAATGCTCTAGCTATTGTCGCCGACGTCTCGCAAGTTCTCGGCGACTATGGCCTGCAATTGAAGGTACTGTACATATATACATATGCATACTTGAATTAAATCACCTCTGTAACTCAAAAGTTTTACTAATTTAAATTTTATTGATAGGTCCAACCAACTGGACGACGTTTGCTTGGAAACACAGAAGTGGGGAGCGATGGGTTCCCCACATGGTTGTCAGGAGCAGACAGGAGGCTGTTGGCGGCAGCGAAAGGAGGAGGAGGAGGAAAAGTGACGCCCAATGCGGTGGTGGCGAAGGACGGGAGCGGGCAGTACAAGACCATTGCTGCAGCATTGGCTGCAGCCCCAAAGAATTCCAAGACACGACACGTGATCTACGTGAAAGCGGGGATCTATAATGAGTTTATCATTGTGACTAAAGACTATACAAACATTTTCATGTACGGAGATGGCCCGAGAAAAACCATCGTCACTGGCCGCAAGTCCTTCCGTGACGGCATTACCACCATGAACACCGCTACCTTCTGTAAGATGTTTTTTTCAAAATCACTCATGCTACCATCATTCTCAAACACACTTTTACTTAATAATTACTACTATAATTACAGCGGCCGTTGGAAATGGCTTCCTCTGCAAAGCAATGGGATTCCAAAACACAGCCGGCCCGGAAGGGCACCAAGCGGTGGCTCTCCGCGTCCAGTCCGACAGATCGGCATTCTTCAACTGCCGAATGGACGGCTACCAAGACACTCTCTACGTGCATGCTAACCGTCAATTCTACCGTAACTGCGTCATCTCCGGCACCGTGGACTTCATCTTCGGCGACTCGAACACGATCATCCAGAACAGCCTGATCATCGTCCGGCGGCCGATGGACAACCAGCAGAACACGGTGACCGCCAACGGGCGCAAGGAAAACAAGGAGTTCAGCGGGCTGGTGATCCACAACTGCCGAATCGTGCCGGAGCAGAAGCTGTTCGCGGACCGGTTCAAGATCCCCACATTCTTGGGGCGCCCGTGGAAGGAGTACGCGTTGACGGTGATCATGGAGACGACATTGGGGGATTTCATCCAGCCGGCCGGGTATATGCCGTGGCAAGGTACATTTGCCCTAGACACGTGTCAGTACCTGGAGTATGCAAACAGGGGACCGGGTGCCAACACTGCAAGGAGAGTGAAGTGGAAGGGGGTTAGGGTTATGGGAAGGAACGAGGCTCTTAAGTTTACGGCTGGATCGCCTTTTCTCCTCGGGAAGGAGTGGCTTCCAGCCACTGGGGGAAACTTCTTACTTGGATTGAAGAACTAA

mRNA sequence

ATGGGGACAGCGGTGGTGTCGGTTCTATCACTTATTTTAGTGGTGGGAGTTGCTTTGGCAGTTGTGGCAGTTGTTAACAGGAACCACGGCAGTTTTGACGGCGAGAATTTGTCCCCCAAAATGAAAGCTGTGGCCACTATATGTTCTCATACTGATTACCAAGAAGAGTGTCAAAATACTCTGGGCAGCATCGCCCACAATTCCTCCTCGGACGACCCGAAGGAGTACATTAAATCAGCGATTATGGCCACTGTTGAGGAGTTTAAGAAAGGGTTCAAATTGACGGACTCCCTTATGGTGGAGGCTGGTAACAATTCCTTCATGAAAATGTCGGTTGAAGATTGCAAAGATTTGCTGCAGTTTGCCGTTGAAGAGCTTCAGGCTTCTTACTCCTCCGTCGGGGACAGCGATGAGCGCTCCCAAGAGGAGCGAGTCGCTGACATCAATACCTGGCTCAGCGCCGTCATTTCTTATCAGCAATCTTGCCTCGACGGCCTCGGAGAAGCCAACCCCAAGCTCAAAGAAACCATGGAAGGTGGTATTGACATTGCACGCAAGCTTACCAGCAATGCTCTAGCTATTGTCGCCGACGTCTCGCAAGTTCTCGGCGACTATGGCCTGCAATTGAAGGTCCAACCAACTGGACGACGTTTGCTTGGAAACACAGAAGTGGGGAGCGATGGGTTCCCCACATGGTTGTCAGGAGCAGACAGGAGGCTGTTGGCGGCAGCGAAAGGAGGAGGAGGAGGAAAAGTGACGCCCAATGCGGTGGTGGCGAAGGACGGGAGCGGGCAGTACAAGACCATTGCTGCAGCATTGGCTGCAGCCCCAAAGAATTCCAAGACACGACACGTGATCTACGTGAAAGCGGGGATCTATAATGAGTTTATCATTGTGACTAAAGACTATACAAACATTTTCATGTACGGAGATGGCCCGAGAAAAACCATCGTCACTGGCCGCAAGTCCTTCCGTGACGGCATTACCACCATGAACACCGCTACCTTCTCGGCCGTTGGAAATGGCTTCCTCTGCAAAGCAATGGGATTCCAAAACACAGCCGGCCCGGAAGGGCACCAAGCGGTGGCTCTCCGCGTCCAGTCCGACAGATCGGCATTCTTCAACTGCCGAATGGACGGCTACCAAGACACTCTCTACGTGCATGCTAACCGTCAATTCTACCGTAACTGCGTCATCTCCGGCACCGTGGACTTCATCTTCGGCGACTCGAACACGATCATCCAGAACAGCCTGATCATCGTCCGGCGGCCGATGGACAACCAGCAGAACACGGTGACCGCCAACGGGCGCAAGGAAAACAAGGAGTTCAGCGGGCTGGTGATCCACAACTGCCGAATCGTGCCGGAGCAGAAGCTGTTCGCGGACCGGTTCAAGATCCCCACATTCTTGGGGCGCCCGTGGAAGGAGTACGCGTTGACGGTGATCATGGAGACGACATTGGGGGATTTCATCCAGCCGGCCGGGTATATGCCGTGGCAAGGTACATTTGCCCTAGACACGTGTCAGTACCTGGAGTATGCAAACAGGGGACCGGGTGCCAACACTGCAAGGAGAGTGAAGTGGAAGGGGGTTAGGGTTATGGGAAGGAACGAGGCTCTTAAGTTTACGGCTGGATCGCCTTTTCTCCTCGGGAAGGAGTGGCTTCCAGCCACTGGGGGAAACTTCTTACTTGGATTGAAGAACTAA

Coding sequence (CDS)

ATGGGGACAGCGGTGGTGTCGGTTCTATCACTTATTTTAGTGGTGGGAGTTGCTTTGGCAGTTGTGGCAGTTGTTAACAGGAACCACGGCAGTTTTGACGGCGAGAATTTGTCCCCCAAAATGAAAGCTGTGGCCACTATATGTTCTCATACTGATTACCAAGAAGAGTGTCAAAATACTCTGGGCAGCATCGCCCACAATTCCTCCTCGGACGACCCGAAGGAGTACATTAAATCAGCGATTATGGCCACTGTTGAGGAGTTTAAGAAAGGGTTCAAATTGACGGACTCCCTTATGGTGGAGGCTGGTAACAATTCCTTCATGAAAATGTCGGTTGAAGATTGCAAAGATTTGCTGCAGTTTGCCGTTGAAGAGCTTCAGGCTTCTTACTCCTCCGTCGGGGACAGCGATGAGCGCTCCCAAGAGGAGCGAGTCGCTGACATCAATACCTGGCTCAGCGCCGTCATTTCTTATCAGCAATCTTGCCTCGACGGCCTCGGAGAAGCCAACCCCAAGCTCAAAGAAACCATGGAAGGTGGTATTGACATTGCACGCAAGCTTACCAGCAATGCTCTAGCTATTGTCGCCGACGTCTCGCAAGTTCTCGGCGACTATGGCCTGCAATTGAAGGTCCAACCAACTGGACGACGTTTGCTTGGAAACACAGAAGTGGGGAGCGATGGGTTCCCCACATGGTTGTCAGGAGCAGACAGGAGGCTGTTGGCGGCAGCGAAAGGAGGAGGAGGAGGAAAAGTGACGCCCAATGCGGTGGTGGCGAAGGACGGGAGCGGGCAGTACAAGACCATTGCTGCAGCATTGGCTGCAGCCCCAAAGAATTCCAAGACACGACACGTGATCTACGTGAAAGCGGGGATCTATAATGAGTTTATCATTGTGACTAAAGACTATACAAACATTTTCATGTACGGAGATGGCCCGAGAAAAACCATCGTCACTGGCCGCAAGTCCTTCCGTGACGGCATTACCACCATGAACACCGCTACCTTCTCGGCCGTTGGAAATGGCTTCCTCTGCAAAGCAATGGGATTCCAAAACACAGCCGGCCCGGAAGGGCACCAAGCGGTGGCTCTCCGCGTCCAGTCCGACAGATCGGCATTCTTCAACTGCCGAATGGACGGCTACCAAGACACTCTCTACGTGCATGCTAACCGTCAATTCTACCGTAACTGCGTCATCTCCGGCACCGTGGACTTCATCTTCGGCGACTCGAACACGATCATCCAGAACAGCCTGATCATCGTCCGGCGGCCGATGGACAACCAGCAGAACACGGTGACCGCCAACGGGCGCAAGGAAAACAAGGAGTTCAGCGGGCTGGTGATCCACAACTGCCGAATCGTGCCGGAGCAGAAGCTGTTCGCGGACCGGTTCAAGATCCCCACATTCTTGGGGCGCCCGTGGAAGGAGTACGCGTTGACGGTGATCATGGAGACGACATTGGGGGATTTCATCCAGCCGGCCGGGTATATGCCGTGGCAAGGTACATTTGCCCTAGACACGTGTCAGTACCTGGAGTATGCAAACAGGGGACCGGGTGCCAACACTGCAAGGAGAGTGAAGTGGAAGGGGGTTAGGGTTATGGGAAGGAACGAGGCTCTTAAGTTTACGGCTGGATCGCCTTTTCTCCTCGGGAAGGAGTGGCTTCCAGCCACTGGGGGAAACTTCTTACTTGGATTGAAGAACTAA

Protein sequence

MGTAVVSVLSLILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNTLGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQFAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGGIDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRLLAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVTKDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALTVIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEALKFTAGSPFLLGKEWLPATGGNFLLGLKN

Homology

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BLAST of MS027560 vs. NCBI nr
Match: XP_022146333.1 (pectinesterase-like [Momordica charantia])

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0


Query: 1   MGTAVVSVLSLILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNT 60
           MGTAVVSVLSLILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNT
Sbjct: 1   MGTAVVSVLSLILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNT 60

Query: 61  LGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQ 120
           LGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQ
Sbjct: 61  LGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQ 120

Query: 121 FAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGG 180
           FAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGG
Sbjct: 121 FAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGG 180

Query: 181 IDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRL 240
           IDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRL
Sbjct: 181 IDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRL 240

Query: 241 LAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVT 300
           LAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVT
Sbjct: 241 LAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVT 300

Query: 301 KDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQ 360
           KDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQ
Sbjct: 301 KDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALT 480
           VRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALT
Sbjct: 421 VRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALT 480

Query: 481 VIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEAL 540
           VIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEAL
Sbjct: 481 VIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEAL 540

Query: 541 KFTAGSPFLLGKEWLPATGGNFLLGLKN 569
           KFTAGSPFLLGKEWLPATGGNFLLGLKN
Sbjct: 541 KFTAGSPFLLGKEWLPATGGNFLLGLKN 568

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BLAST of MS027560 vs. NCBI nr
Match: XP_022146322.1 (pectinesterase-like [Momordica charantia])

HSP 1 Score: 916.4 bits (2367), Expect = 1.2e-262
Identity = 468/573 (81.68%), Postives = 512/573 (89.35%), Query Frame = 0


Query: 1   MGTAVVSVLSLILVVGVALAVVAVVNRN-HGSFDGENLSPKMKAVATICSHTDYQEECQN 60
           +G A VSVLSLILVVGVALAVVAVVN N  GS D EN+SPKMKA+A+ICS TDYQ+ECQ+
Sbjct: 2   VGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQD 61

Query: 61  TLGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLL 120
           TLG +A NS+SDDPKEYIK+AIMATV+E KKGF LTDSLMVEAGN++FMKMSVEDCKDLL
Sbjct: 62  TLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDLL 121

Query: 121 QFAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETM-E 180
           +FAV ELQASYSSVG +DERSQEERV+DINTWLSAVISYQ+SCLD LGE+NPKLKETM  
Sbjct: 122 RFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMGA 181

Query: 181 GGIDIARKLTSNALAIVADVSQVLGDYGLQLKV-QPTGRRLLGNTEVGSDGFPTWLSGAD 240
           G +D A KLTSNALAI ++VS++LG+YGLQLK  QP GRRLLG+TEVGSDGFPTW S AD
Sbjct: 182 GSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKAD 241

Query: 241 RRLLAAAKGGGGG--KVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNE 300
           R+LLAAA+ GGGG   V PN VVAKDGSG YKTI AALAAAPKNSKTR+VIYVKAGIY+E
Sbjct: 242 RKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYDE 301

Query: 301 FIIVTKDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAG 360
           ++ VT D T IFMYGDGPRKTIVTGRKS RDGI TMNTATF+AVG  FLCKAMGFQNTAG
Sbjct: 302 YVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTAG 361

Query: 361 PEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQ 420
           PEGHQAVALRVQSDRSAF+ CR+DGYQD+LY   NRQFYRNCVISGTVDFIFG+S+TIIQ
Sbjct: 362 PEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQ 421

Query: 421 NSLIIVRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK 480
           NSLIIVRRPM  QQNTVTA+GRKEN+E SGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK
Sbjct: 422 NSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK 481

Query: 481 EYALTVIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMG 540
           +YALTVIME+TLGD IQPAGYMPW+   + DTCQYLEYANRGPGANTARRVKWKGVRV+G
Sbjct: 482 KYALTVIMESTLGDLIQPAGYMPWEED-SRDTCQYLEYANRGPGANTARRVKWKGVRVIG 541

Query: 541 RNEALKFTAGSPFLLGKEWLPATGGNFLLGLKN 569
           RNEALKFT GSPFL+G  WLP T G FL GLKN
Sbjct: 542 RNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 573

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BLAST of MS027560 vs. NCBI nr
Match: XP_008466382.2 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 882.1 bits (2278), Expect = 2.6e-252
Identity = 433/557 (77.74%), Postives = 491/557 (88.15%), Query Frame = 0


Query: 12  ILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNTLGSIAHNSSSD 71
           +LVVGV LAV A+VN+NH S +  ++SPKMKAV++ICS+TDYQ+ECQ TLG++ HNSS D
Sbjct: 6   LLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTTLGNVGHNSSVD 65

Query: 72  DPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQFAVEELQASYS 131
           DPKE++K+AI AT+EE KKG+ LTD+LMVEA NN+ +KM+V+DC+DLLQ A++EL ASYS
Sbjct: 66  DPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDELHASYS 125

Query: 132 SVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGGIDIARKLTSNA 191
           +VGD D  + E+RVADI  WL+AVISYQQSCLDGL E +P+LK+ M+GG+D+A KLTSNA
Sbjct: 126 TVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAKLTSNA 185

Query: 192 LAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRLLAAAKGGGGGK 251
           LAIV  VS +L   GLQLKVQP+GRRLLG TEV +DGFPTWL+GADR+LLAA +  GG +
Sbjct: 186 LAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADRKLLAAKR--GGVR 245

Query: 252 VTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVTKDYTNIFMYGD 311
           V PNAVVAKDGSGQYKTIAAALAA PK  K R+VIYVKAGIYNE+IIVTKD  N+FMYGD
Sbjct: 246 VKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFMYGD 305

Query: 312 GPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQAVALRVQSDRS 371
           GPRKTIVTGRKS RDGI T NTATF+A+G GFLCK+MGF NTAGPEGHQAVALRVQSDRS
Sbjct: 306 GPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRS 365

Query: 372 AFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNT 431
           AFFNCRMDGYQDTLYV  +RQFYRNCV+SGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNT
Sbjct: 366 AFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNT 425

Query: 432 VTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALTVIMETTLGDFI 491
           VTANGR + KE SGLVIHNCRIVPEQKLFA+RFKIPTFLGRPWKEYA TVIMETTLGDFI
Sbjct: 426 VTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLGDFI 485

Query: 492 QPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEALKFTAGSPFLLG 551
           QPAGYMPW G FAL TC Y EY NRGPGANT RRV+WKGVRV+GRNEA++FTAG PFLLG
Sbjct: 486 QPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAG-PFLLG 545

Query: 552 KEWLPATGGNFLLGLKN 569
           + WLPATGG +LLGLKN
Sbjct: 546 RAWLPATGGPYLLGLKN 559

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BLAST of MS027560 vs. NCBI nr
Match: KAA0050276.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98131.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 880.9 bits (2275), Expect = 5.7e-252
Identity = 435/568 (76.58%), Postives = 496/568 (87.32%), Query Frame = 0


Query: 1   MGTAVVSVLSLILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNT 60
           M   VV  ++ +LVVGV LAV A+VN+NH S +  ++SPKMKAV++ICS+TDYQ+ECQ T
Sbjct: 1   MVAKVVMAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTT 60

Query: 61  LGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQ 120
           LG++ HNSS DDPKE++K+AI AT+EE KKG+ LTD+LMVEA NN+ +KM+V+DC+DLLQ
Sbjct: 61  LGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQ 120

Query: 121 FAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGG 180
            A++EL ASYS+VGD D  + E+RVADI  WL+AVISYQQSCLDGL E +P+LK+ M+GG
Sbjct: 121 SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGG 180

Query: 181 IDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRL 240
           +D+A KLTSNALAIV  VS +L   GLQLKVQP+GRRLLG TEV +DG+PTWL+GADR+L
Sbjct: 181 LDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKL 240

Query: 241 LAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVT 300
           LAA +  GG +V PNAVVAKDGSGQYKTIAAALAA PK  K R+VIYVKAGIYNE+IIVT
Sbjct: 241 LAAKR--GGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300

Query: 301 KDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQ 360
           KD  N+FMYGDGPRKTIVTGRKS RDGI T NTATF+A+G GFLCK+MGF NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYV  +RQFYRNCV+SGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALT 480
           VRRPMDNQQNTVTANGR + KE SGLVIHNCRIVPEQKLFA+RFKIPTFLGRPWKEYA T
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEAL 540
           VIMETTLGDFIQPAGYMPW G FAL TC Y EY NRGPGANT RRV+WKGVRV+GRNEA+
Sbjct: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540

Query: 541 KFTAGSPFLLGKEWLPATGGNFLLGLKN 569
           +FTAG PFLLG+ WLPATGG +LLGLKN
Sbjct: 541 QFTAG-PFLLGRAWLPATGGPYLLGLKN 565

[Close]

BLAST of MS027560 vs. NCBI nr
Match: KGN49779.1 (hypothetical protein Csa_017783 [Cucumis sativus])

HSP 1 Score: 875.5 bits (2261), Expect = 2.4e-250
Identity = 437/568 (76.94%), Postives = 494/568 (86.97%), Query Frame = 0


Query: 1   MGTAVVSVLSLILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNT 60
           +G  V+S +SLILVVGVALAVVA+VN++  S +  NLSPKMKAVA+ICS+ DYQ ECQ T
Sbjct: 2   VGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQTT 61

Query: 61  LGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQ 120
           LGS+ +NSS +DPKE+IK+AI +T+EE KKG+ LTD+LMVEA NN+ +KMSV+DCKDLLQ
Sbjct: 62  LGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQ 121

Query: 121 FAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGG 180
            A++EL ASYS+VGD D  + E+R+ADI  WL+AVISYQQSCLDGL E +P+LK+ M+ G
Sbjct: 122 SAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDG 181

Query: 181 IDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRL 240
           +D+A KLTSNALAIV  VS +L + GLQLKVQP+GRRLLG TEV SD FPTWL+G+DR+L
Sbjct: 182 LDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKL 241

Query: 241 LAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVT 300
           LAA +  GG +V PN VVAKDGSGQYKTI AALAA PK  K R+VIYVKAG+YNE IIVT
Sbjct: 242 LAAKR--GGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 301

Query: 301 KDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQ 360
           KD  NIFMYGDGPRKTIVTGRKS RDGITT NTA+F+A+G GFLCK+MGF NTAGPEGHQ
Sbjct: 302 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 361

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYV  +RQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 362 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 421

Query: 421 VRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALT 480
           VRRPMDNQQNTVTANGR + KE SGLVIHNCRIVPEQKLFA+RFKIPTFLGRPWKEYA T
Sbjct: 422 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 481

Query: 481 VIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEAL 540
           VIME+TLGDFIQPAGYMPW G FAL TC Y EY NRGPGANT RRV+WKGVRV+GRNEA+
Sbjct: 482 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 541

Query: 541 KFTAGSPFLLGKEWLPATGGNFLLGLKN 569
           +FTAG PFLLGK WLP TGG +LLGLKN
Sbjct: 542 QFTAG-PFLLGKAWLPGTGGPYLLGLKN 566

[Close]

BLAST of MS027560 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 9.5e-125
Identity = 234/564 (41.49%), Postives = 356/564 (63.12%), Query Frame = 0


Query: 5   VVSVLSLILV-VGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNTLGS 64
           ++S+ S++L+ + VA+ +   VN++  + D E ++  +KA+  +C+ TDY+E C++TL  
Sbjct: 18  IISISSVLLISMVVAVTIGVSVNKSDNAGD-EEITTSVKAIKDVCAPTDYKETCEDTLRK 77

Query: 65  IAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQFAV 124
            A ++S  DP E +K+A  AT+++     K + + M+E   +   KM+++ CK+L+ +A+
Sbjct: 78  DAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDYAI 137

Query: 125 EELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGGIDI 184
            EL  S+  +G  +    +E +  +  WLSA IS++Q+CLDG         ET++  +  
Sbjct: 138 GELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKALKT 197

Query: 185 ARKLTSNALAIVADVSQVLGDYGLQLKV-QPTGRRLLGNTEVGSDGFPTWLSGADRRLLA 244
           A +LT N LA+V ++S  LG    Q+++ +   RRLL      S  FP+W+    RRLL 
Sbjct: 198 AVQLTHNGLAMVTEMSNYLG----QMQIPEMNSRRLL------SQEFPSWMDARARRLLN 257

Query: 245 AAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVTKD 304
           A       +V P+ VVA+DGSGQYKTI  AL   PK   T  V+++K GIY E++ V + 
Sbjct: 258 APM----SEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRS 317

Query: 305 YTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQAV 364
            T++   GDGP KT+++G KS++DGITT  TAT + VG+ F+ K + F+NTAG   HQAV
Sbjct: 318 MTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAV 377

Query: 365 ALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVR 424
           A+RV +D S F+NC+ DGYQDTLY H++RQFYR+C ISGT+DF+FGD+  + QN  ++VR
Sbjct: 378 AIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVR 437

Query: 425 RPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALTVI 484
           +P+ NQ   +TA+GRK+ +E +G V+  C IV E    A + +  T+LGRPWKEY+ T+I
Sbjct: 438 KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTII 497

Query: 485 METTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEALKF 544
           M T + DF+ P G+ PW G F L+T  Y E  N GPGA   +RV W G++ +   E LKF
Sbjct: 498 MNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKF 557

Query: 545 TAGSPFLLGKEWLPATGGNFLLGL 567
           T  + ++ G  W+P  G  ++LGL
Sbjct: 558 TP-AQYIQGDAWIPGKGVPYILGL 562

[Close]

BLAST of MS027560 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 448.4 bits (1152), Expect = 1.2e-124
Identity = 232/565 (41.06%), Postives = 358/565 (63.36%), Query Frame = 0


Query: 5   VVSVLSLILV-VGVALAVVAVVNRNHGSFDGE-NLSPKMKAVATICSHTDYQEECQNTLG 64
           V+++ S++L+ + VA+ V   +N++ G   G+  ++  +KAV  +C+ TDY++ C++TL 
Sbjct: 17  VITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTL- 76

Query: 65  SIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQFA 124
            I +  ++ DP E +K+A   T+++     K + ++M E   +S  +M+++ CK+L+ +A
Sbjct: 77  -IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYA 136

Query: 125 VEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGGID 184
           ++EL  S+  +G  +    +E + ++  WLSA IS++++CL+G         ETM+  + 
Sbjct: 137 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 196

Query: 185 IARKLTSNALAIVADVSQVLGDYGLQLKVQP-TGRRLLGNTEVGSDGFPTWLSGADRRLL 244
            A +LT N LAI++++S  +G    Q+++     RRLL      ++GFP+W+    R+LL
Sbjct: 197 TAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRLL------AEGFPSWVDQRGRKLL 256

Query: 245 AAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVTK 304
            AA       V P+ VVA+DGSGQYKTI  AL   PK   T  V+++KAG+Y E++ V K
Sbjct: 257 QAA--AAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNK 316

Query: 305 DYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQA 364
             +++   GDGP KTI++G K+++DGITT  TAT + VGN F+ K +GF+NTAG   HQA
Sbjct: 317 TMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQA 376

Query: 365 VALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 424
           VA+RVQSD S FFNCR DGYQDTLY H++RQF+R+C ISGT+DF+FGD+  + QN  ++V
Sbjct: 377 VAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLV 436

Query: 425 RRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALTV 484
           R+P+ NQ   +TA+GRK+ +E +G V   C I  E    A +     +LGRPWKEY+ T+
Sbjct: 437 RKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTI 496

Query: 485 IMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEALK 544
           IM T + DF+QP G+ PW G F L T  Y E  N GPG+  A RV W G++ +   + LK
Sbjct: 497 IMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILK 556

Query: 545 FTAGSPFLLGKEWLPATGGNFLLGL 567
           FT  + ++ G +W+P  G  +  GL
Sbjct: 557 FTP-AQYIQGDDWIPGKGVPYTTGL 565

[Close]

BLAST of MS027560 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 7.5e-122
Identity = 244/530 (46.04%), Postives = 330/530 (62.26%), Query Frame = 0


Query: 42  KAVATICSHTDYQEECQNTL-GSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMV 101
           K + T+C+ T Y+  CQNTL      ++   DP+  +KSAI+A  ++  + FK   SL  
Sbjct: 93  KIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKT 152

Query: 102 EAGNNSFMKMSVEDCKDLLQFAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQ 161
           E   N   K ++  CK L+  A EEL  S   + DS+  +  + V D+++WLSAV+SYQ+
Sbjct: 153 E---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSWLSAVMSYQE 212

Query: 162 SCLDGLGEANPKLKETMEGGIDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLL- 221
           +C+DG  E   KLK  +    + ++ LTSN+LA++  +       G    V     RLL 
Sbjct: 213 TCVDGFEEG--KLKTEIRKNFNSSQVLTSNSLAMIKSLD------GYLSSVPKVKTRLLL 272

Query: 222 --GNTEVGSDGFPTWLSGADRRLLAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAP 281
              ++   +D   +WLS  +RR+L A        + PNA VAKDGSG + TI AAL A P
Sbjct: 273 EARSSAKETDHITSWLSNKERRMLKAVDVKA---LKPNATVAKDGSGNFTTINAALKAMP 332

Query: 282 KNSKTRHVIYVKAGIYNEFIIVTKDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFS 341
              + R+ IY+K GIY+E +I+ K   N+ M GDG +KTIVTG KS    I T  TATF 
Sbjct: 333 AKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLTATFV 392

Query: 342 AVGNGFLCKAMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNC 401
           A G GF+ ++MGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY + +RQ+YR+C
Sbjct: 393 AQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQYYRSC 452

Query: 402 VISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQ 461
           VI GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR +  + +G VIHNC + P +
Sbjct: 453 VIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTVAPNE 512

Query: 462 KLFADRFKIPTFLGRPWKEYALTVIMETTLGDFIQPAGYMPWQGT-FALDTCQYLEYANR 521
            L   + +  ++LGRPWK ++ TV+ME+T+ D I P G++ WQ T FA+DT  Y EY N 
Sbjct: 513 DLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAEYKND 572

Query: 522 GPGANTARRVKWKGVRVMGRNEALKFTAGSPFLLGKEWLPATGGNFLLGL 567
           GP   TA RVKW G RV+ + EA+KFT G PFL G EW+ A G    LGL
Sbjct: 573 GPSGATAARVKWPGFRVLNKEEAMKFTVG-PFLQG-EWIQAIGSPVKLGL 606

[Close]

BLAST of MS027560 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 428.3 bits (1100), Expect = 1.3e-118
Identity = 236/527 (44.78%), Postives = 331/527 (62.81%), Query Frame = 0


Query: 42  KAVATICSHTDYQEECQNTLGSIAHNS-SSDDPKEYIKSAIMATVEEFKKGFKLTDSLMV 101
           K + T+CS T Y + C+ TL +      + D+P  ++KSAI A  E+     +   SL  
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKT 166

Query: 102 EAGNNSFMKMSVEDCKDLLQFAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQ 161
           E   N   K ++E CK L++ A EE  AS + +  ++  S E+ V D+ +WLSAV+SYQ+
Sbjct: 167 E---NQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 162 SCLDGLGEANPKLKETMEGGIDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLG 221
           +CLDG  E N  LK  ++  ++ ++ LTSN+LA++   ++      L   ++   R LL 
Sbjct: 227 TCLDGFEEGN--LKSEVKTSVNSSQVLTSNSLALIKTFTE-----NLSPVMKVVERHLL- 286

Query: 222 NTEVGSDGFPTWLSGADRRLLAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNS 281
                 D  P+W+S  DRR+L A        + PNA VAKDGSG + TI  AL A P+  
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVDVKA---LKPNATVAKDGSGDFTTINDALRAMPEKY 346

Query: 282 KTRHVIYVKAGIYNEFIIVTKDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVG 341
           + R++IYVK GIY+E++ V K   N+ M GDG +KTIVTG KS    I T  TATF A G
Sbjct: 347 EGRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQG 406

Query: 342 NGFLCKAMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVIS 401
            GF+ ++MGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY + +RQ+YR+CVI 
Sbjct: 407 EGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIV 466

Query: 402 GTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLF 461
           GT+DFIFGD+  I QN  I +R+ +  Q+NTVTA GR +  + +G V+HNC+I   + L 
Sbjct: 467 GTIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLK 526

Query: 462 ADRFKIPTFLGRPWKEYALTVIMETTLGDFIQPAGYMPWQGT-FALDTCQYLEYANRGPG 521
             + +  ++LGRPWK Y+ T+IME+ + + I P G++ WQ T FA+DT  Y EY N+G  
Sbjct: 527 PVKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSS 586

Query: 522 ANTARRVKWKGVRVMGRNEALKFTAGSPFLLGKEWLPATGGNFLLGL 567
            +T  RVKW G +V+ + EAL +T G PFL G +W+ A+G    LGL
Sbjct: 587 GDTTSRVKWPGFKVINKEEALNYTVG-PFLQG-DWISASGSPVKLGL 611

[Close]

BLAST of MS027560 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 1.3e-118
Identity = 232/555 (41.80%), Postives = 336/555 (60.54%), Query Frame = 0


Query: 8   VLSLILVVGVALAVVAVVNRNHG-SFDGENLSPKMKAVATICSHTDYQEECQNTLGSIAH 67
           + +L++++ VA+ +    N +H        +     AV  +C+ TDY+E C N+L  +  
Sbjct: 18  ITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNSL--MKA 77

Query: 68  NSSSDDPKEYIKSAIMATVEEFKKGFKLTD-SLMVEAGNNSFMKMSVEDCKDLLQFAVEE 127
           +  S  P + IK     T+   +   K     L  +A N+   K ++E C+ L+  A ++
Sbjct: 78  SPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLMNDATDD 137

Query: 128 LQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGGIDIAR 187
           L+    +         E+ V D+  WLS  I+YQQ+C+D   E N KL + M+     +R
Sbjct: 138 LKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFKTSR 197

Query: 188 KLTSNALAIVADVSQVLGDY---GLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRLLA 247
           +LTSN LA++ ++S +LG++   G+   +    R+LL       DG P+W+ G + R L 
Sbjct: 198 ELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS----AEDGIPSWV-GPNTRRLM 257

Query: 248 AAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVTKD 307
           A KGG    V  N VVA DGSGQYKTI  AL A PK ++   VIY+K G+YNE + VTK 
Sbjct: 258 ATKGG----VKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKK 317

Query: 308 YTNIFMYGDGPRKTIVTGRKSFRDG-ITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQA 367
            T++   GDGP KT +TG  ++  G + T  TAT +  G+ F  K +GF+NTAGPEGHQA
Sbjct: 318 MTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQA 377

Query: 368 VALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 427
           VALRV +D + F+NC++DGYQDTLYVH++RQF+R+C +SGTVDFIFGD   ++QN  I+V
Sbjct: 378 VALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVV 437

Query: 428 RRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALTV 487
           R+PM +Q   +TA GR + +E +GLV+ NC I  E      +     +LGRPWKE++ T+
Sbjct: 438 RKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTI 497

Query: 488 IMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEALK 547
           IM TT+ D I PAG++PW G FAL+T  Y EY N GPG+N A+RVKW G++ +   +AL+
Sbjct: 498 IMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALR 557

Query: 548 FTAGSPFLLGKEWLP 557
           FT  + FL G  W+P
Sbjct: 558 FTP-ARFLRGNLWIP 560

[Close]

BLAST of MS027560 vs. ExPASy TrEMBL
Match: A0A6J1CYB2 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015570 PE=3 SV=1)

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0


Query: 1   MGTAVVSVLSLILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNT 60
           MGTAVVSVLSLILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNT
Sbjct: 1   MGTAVVSVLSLILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNT 60

Query: 61  LGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQ 120
           LGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQ
Sbjct: 61  LGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQ 120

Query: 121 FAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGG 180
           FAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGG
Sbjct: 121 FAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGG 180

Query: 181 IDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRL 240
           IDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRL
Sbjct: 181 IDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRL 240

Query: 241 LAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVT 300
           LAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVT
Sbjct: 241 LAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVT 300

Query: 301 KDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQ 360
           KDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQ
Sbjct: 301 KDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALT 480
           VRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALT
Sbjct: 421 VRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALT 480

Query: 481 VIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEAL 540
           VIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEAL
Sbjct: 481 VIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEAL 540

Query: 541 KFTAGSPFLLGKEWLPATGGNFLLGLKN 569
           KFTAGSPFLLGKEWLPATGGNFLLGLKN
Sbjct: 541 KFTAGSPFLLGKEWLPATGGNFLLGLKN 568

[Close]

BLAST of MS027560 vs. ExPASy TrEMBL
Match: A0A6J1CZA2 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015559 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.9e-263
Identity = 468/573 (81.68%), Postives = 512/573 (89.35%), Query Frame = 0


Query: 1   MGTAVVSVLSLILVVGVALAVVAVVNRN-HGSFDGENLSPKMKAVATICSHTDYQEECQN 60
           +G A VSVLSLILVVGVALAVVAVVN N  GS D EN+SPKMKA+A+ICS TDYQ+ECQ+
Sbjct: 2   VGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQD 61

Query: 61  TLGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLL 120
           TLG +A NS+SDDPKEYIK+AIMATV+E KKGF LTDSLMVEAGN++FMKMSVEDCKDLL
Sbjct: 62  TLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDLL 121

Query: 121 QFAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETM-E 180
           +FAV ELQASYSSVG +DERSQEERV+DINTWLSAVISYQ+SCLD LGE+NPKLKETM  
Sbjct: 122 RFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMGA 181

Query: 181 GGIDIARKLTSNALAIVADVSQVLGDYGLQLKV-QPTGRRLLGNTEVGSDGFPTWLSGAD 240
           G +D A KLTSNALAI ++VS++LG+YGLQLK  QP GRRLLG+TEVGSDGFPTW S AD
Sbjct: 182 GSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKAD 241

Query: 241 RRLLAAAKGGGGG--KVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNE 300
           R+LLAAA+ GGGG   V PN VVAKDGSG YKTI AALAAAPKNSKTR+VIYVKAGIY+E
Sbjct: 242 RKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYDE 301

Query: 301 FIIVTKDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAG 360
           ++ VT D T IFMYGDGPRKTIVTGRKS RDGI TMNTATF+AVG  FLCKAMGFQNTAG
Sbjct: 302 YVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTAG 361

Query: 361 PEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQ 420
           PEGHQAVALRVQSDRSAF+ CR+DGYQD+LY   NRQFYRNCVISGTVDFIFG+S+TIIQ
Sbjct: 362 PEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQ 421

Query: 421 NSLIIVRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK 480
           NSLIIVRRPM  QQNTVTA+GRKEN+E SGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK
Sbjct: 422 NSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK 481

Query: 481 EYALTVIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMG 540
           +YALTVIME+TLGD IQPAGYMPW+   + DTCQYLEYANRGPGANTARRVKWKGVRV+G
Sbjct: 482 KYALTVIMESTLGDLIQPAGYMPWEED-SRDTCQYLEYANRGPGANTARRVKWKGVRVIG 541

Query: 541 RNEALKFTAGSPFLLGKEWLPATGGNFLLGLKN 569
           RNEALKFT GSPFL+G  WLP T G FL GLKN
Sbjct: 542 RNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 573

[Close]

BLAST of MS027560 vs. ExPASy TrEMBL
Match: A0A1S3CR40 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503798 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.2e-252
Identity = 433/557 (77.74%), Postives = 491/557 (88.15%), Query Frame = 0


Query: 12  ILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNTLGSIAHNSSSD 71
           +LVVGV LAV A+VN+NH S +  ++SPKMKAV++ICS+TDYQ+ECQ TLG++ HNSS D
Sbjct: 6   LLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTTLGNVGHNSSVD 65

Query: 72  DPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQFAVEELQASYS 131
           DPKE++K+AI AT+EE KKG+ LTD+LMVEA NN+ +KM+V+DC+DLLQ A++EL ASYS
Sbjct: 66  DPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDELHASYS 125

Query: 132 SVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGGIDIARKLTSNA 191
           +VGD D  + E+RVADI  WL+AVISYQQSCLDGL E +P+LK+ M+GG+D+A KLTSNA
Sbjct: 126 TVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAKLTSNA 185

Query: 192 LAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRLLAAAKGGGGGK 251
           LAIV  VS +L   GLQLKVQP+GRRLLG TEV +DGFPTWL+GADR+LLAA +  GG +
Sbjct: 186 LAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADRKLLAAKR--GGVR 245

Query: 252 VTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVTKDYTNIFMYGD 311
           V PNAVVAKDGSGQYKTIAAALAA PK  K R+VIYVKAGIYNE+IIVTKD  N+FMYGD
Sbjct: 246 VKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFMYGD 305

Query: 312 GPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQAVALRVQSDRS 371
           GPRKTIVTGRKS RDGI T NTATF+A+G GFLCK+MGF NTAGPEGHQAVALRVQSDRS
Sbjct: 306 GPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRS 365

Query: 372 AFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNT 431
           AFFNCRMDGYQDTLYV  +RQFYRNCV+SGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNT
Sbjct: 366 AFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNT 425

Query: 432 VTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALTVIMETTLGDFI 491
           VTANGR + KE SGLVIHNCRIVPEQKLFA+RFKIPTFLGRPWKEYA TVIMETTLGDFI
Sbjct: 426 VTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLGDFI 485

Query: 492 QPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEALKFTAGSPFLLG 551
           QPAGYMPW G FAL TC Y EY NRGPGANT RRV+WKGVRV+GRNEA++FTAG PFLLG
Sbjct: 486 QPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAG-PFLLG 545

Query: 552 KEWLPATGGNFLLGLKN 569
           + WLPATGG +LLGLKN
Sbjct: 546 RAWLPATGGPYLLGLKN 559

[Close]

BLAST of MS027560 vs. ExPASy TrEMBL
Match: A0A5A7U7W4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090 PE=3 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.8e-252
Identity = 435/568 (76.58%), Postives = 496/568 (87.32%), Query Frame = 0


Query: 1   MGTAVVSVLSLILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNT 60
           M   VV  ++ +LVVGV LAV A+VN+NH S +  ++SPKMKAV++ICS+TDYQ+ECQ T
Sbjct: 1   MVAKVVMAVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQTT 60

Query: 61  LGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQ 120
           LG++ HNSS DDPKE++K+AI AT+EE KKG+ LTD+LMVEA NN+ +KM+V+DC+DLLQ
Sbjct: 61  LGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQ 120

Query: 121 FAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGG 180
            A++EL ASYS+VGD D  + E+RVADI  WL+AVISYQQSCLDGL E +P+LK+ M+GG
Sbjct: 121 SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGG 180

Query: 181 IDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRL 240
           +D+A KLTSNALAIV  VS +L   GLQLKVQP+GRRLLG TEV +DG+PTWL+GADR+L
Sbjct: 181 LDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRKL 240

Query: 241 LAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVT 300
           LAA +  GG +V PNAVVAKDGSGQYKTIAAALAA PK  K R+VIYVKAGIYNE+IIVT
Sbjct: 241 LAAKR--GGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300

Query: 301 KDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQ 360
           KD  N+FMYGDGPRKTIVTGRKS RDGI T NTATF+A+G GFLCK+MGF NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYV  +RQFYRNCV+SGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALT 480
           VRRPMDNQQNTVTANGR + KE SGLVIHNCRIVPEQKLFA+RFKIPTFLGRPWKEYA T
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEAL 540
           VIMETTLGDFIQPAGYMPW G FAL TC Y EY NRGPGANT RRV+WKGVRV+GRNEA+
Sbjct: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540

Query: 541 KFTAGSPFLLGKEWLPATGGNFLLGLKN 569
           +FTAG PFLLG+ WLPATGG +LLGLKN
Sbjct: 541 QFTAG-PFLLGRAWLPATGGPYLLGLKN 565

[Close]

BLAST of MS027560 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 1.2e-250
Identity = 437/568 (76.94%), Postives = 494/568 (86.97%), Query Frame = 0


Query: 1   MGTAVVSVLSLILVVGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNT 60
           +G  V+S +SLILVVGVALAVVA+VN++  S +  NLSPKMKAVA+ICS+ DYQ ECQ T
Sbjct: 2   VGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQTT 61

Query: 61  LGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQ 120
           LGS+ +NSS +DPKE+IK+AI +T+EE KKG+ LTD+LMVEA NN+ +KMSV+DCKDLLQ
Sbjct: 62  LGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQ 121

Query: 121 FAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGG 180
            A++EL ASYS+VGD D  + E+R+ADI  WL+AVISYQQSCLDGL E +P+LK+ M+ G
Sbjct: 122 SAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDG 181

Query: 181 IDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRL 240
           +D+A KLTSNALAIV  VS +L + GLQLKVQP+GRRLLG TEV SD FPTWL+G+DR+L
Sbjct: 182 LDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKL 241

Query: 241 LAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVT 300
           LAA +  GG +V PN VVAKDGSGQYKTI AALAA PK  K R+VIYVKAG+YNE IIVT
Sbjct: 242 LAAKR--GGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 301

Query: 301 KDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQ 360
           KD  NIFMYGDGPRKTIVTGRKS RDGITT NTA+F+A+G GFLCK+MGF NTAGPEGHQ
Sbjct: 302 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 361

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYV  +RQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 362 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 421

Query: 421 VRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALT 480
           VRRPMDNQQNTVTANGR + KE SGLVIHNCRIVPEQKLFA+RFKIPTFLGRPWKEYA T
Sbjct: 422 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 481

Query: 481 VIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEAL 540
           VIME+TLGDFIQPAGYMPW G FAL TC Y EY NRGPGANT RRV+WKGVRV+GRNEA+
Sbjct: 482 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 541

Query: 541 KFTAGSPFLLGKEWLPATGGNFLLGLKN 569
           +FTAG PFLLGK WLP TGG +LLGLKN
Sbjct: 542 QFTAG-PFLLGKAWLPGTGGPYLLGLKN 566

[Close]

BLAST of MS027560 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 448.7 bits (1153), Expect = 6.8e-126
Identity = 234/564 (41.49%), Postives = 356/564 (63.12%), Query Frame = 0


Query: 5   VVSVLSLILV-VGVALAVVAVVNRNHGSFDGENLSPKMKAVATICSHTDYQEECQNTLGS 64
           ++S+ S++L+ + VA+ +   VN++  + D E ++  +KA+  +C+ TDY+E C++TL  
Sbjct: 18  IISISSVLLISMVVAVTIGVSVNKSDNAGD-EEITTSVKAIKDVCAPTDYKETCEDTLRK 77

Query: 65  IAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQFAV 124
            A ++S  DP E +K+A  AT+++     K + + M+E   +   KM+++ CK+L+ +A+
Sbjct: 78  DAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDYAI 137

Query: 125 EELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGGIDI 184
            EL  S+  +G  +    +E +  +  WLSA IS++Q+CLDG         ET++  +  
Sbjct: 138 GELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKALKT 197

Query: 185 ARKLTSNALAIVADVSQVLGDYGLQLKV-QPTGRRLLGNTEVGSDGFPTWLSGADRRLLA 244
           A +LT N LA+V ++S  LG    Q+++ +   RRLL      S  FP+W+    RRLL 
Sbjct: 198 AVQLTHNGLAMVTEMSNYLG----QMQIPEMNSRRLL------SQEFPSWMDARARRLLN 257

Query: 245 AAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVTKD 304
           A       +V P+ VVA+DGSGQYKTI  AL   PK   T  V+++K GIY E++ V + 
Sbjct: 258 APM----SEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRS 317

Query: 305 YTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQAV 364
            T++   GDGP KT+++G KS++DGITT  TAT + VG+ F+ K + F+NTAG   HQAV
Sbjct: 318 MTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAV 377

Query: 365 ALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVR 424
           A+RV +D S F+NC+ DGYQDTLY H++RQFYR+C ISGT+DF+FGD+  + QN  ++VR
Sbjct: 378 AIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVR 437

Query: 425 RPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALTVI 484
           +P+ NQ   +TA+GRK+ +E +G V+  C IV E    A + +  T+LGRPWKEY+ T+I
Sbjct: 438 KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTII 497

Query: 485 METTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEALKF 544
           M T + DF+ P G+ PW G F L+T  Y E  N GPGA   +RV W G++ +   E LKF
Sbjct: 498 MNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKF 557

Query: 545 TAGSPFLLGKEWLPATGGNFLLGL 567
           T  + ++ G  W+P  G  ++LGL
Sbjct: 558 TP-AQYIQGDAWIPGKGVPYILGL 562

[Close]

BLAST of MS027560 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 448.4 bits (1152), Expect = 8.8e-126
Identity = 232/565 (41.06%), Postives = 358/565 (63.36%), Query Frame = 0


Query: 5   VVSVLSLILV-VGVALAVVAVVNRNHGSFDGE-NLSPKMKAVATICSHTDYQEECQNTLG 64
           V+++ S++L+ + VA+ V   +N++ G   G+  ++  +KAV  +C+ TDY++ C++TL 
Sbjct: 17  VITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTL- 76

Query: 65  SIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLLQFA 124
            I +  ++ DP E +K+A   T+++     K + ++M E   +S  +M+++ CK+L+ +A
Sbjct: 77  -IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYA 136

Query: 125 VEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGGID 184
           ++EL  S+  +G  +    +E + ++  WLSA IS++++CL+G         ETM+  + 
Sbjct: 137 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 196

Query: 185 IARKLTSNALAIVADVSQVLGDYGLQLKVQP-TGRRLLGNTEVGSDGFPTWLSGADRRLL 244
            A +LT N LAI++++S  +G    Q+++     RRLL      ++GFP+W+    R+LL
Sbjct: 197 TAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRLL------AEGFPSWVDQRGRKLL 256

Query: 245 AAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVTK 304
            AA       V P+ VVA+DGSGQYKTI  AL   PK   T  V+++KAG+Y E++ V K
Sbjct: 257 QAA--AAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNK 316

Query: 305 DYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQA 364
             +++   GDGP KTI++G K+++DGITT  TAT + VGN F+ K +GF+NTAG   HQA
Sbjct: 317 TMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQA 376

Query: 365 VALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 424
           VA+RVQSD S FFNCR DGYQDTLY H++RQF+R+C ISGT+DF+FGD+  + QN  ++V
Sbjct: 377 VAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLV 436

Query: 425 RRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALTV 484
           R+P+ NQ   +TA+GRK+ +E +G V   C I  E    A +     +LGRPWKEY+ T+
Sbjct: 437 RKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTI 496

Query: 485 IMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEALK 544
           IM T + DF+QP G+ PW G F L T  Y E  N GPG+  A RV W G++ +   + LK
Sbjct: 497 IMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILK 556

Query: 545 FTAGSPFLLGKEWLPATGGNFLLGL 567
           FT  + ++ G +W+P  G  +  GL
Sbjct: 557 FTP-AQYIQGDDWIPGKGVPYTTGL 565

[Close]

BLAST of MS027560 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 439.1 bits (1128), Expect = 5.4e-123
Identity = 244/530 (46.04%), Postives = 330/530 (62.26%), Query Frame = 0


Query: 42  KAVATICSHTDYQEECQNTL-GSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMV 101
           K + T+C+ T Y+  CQNTL      ++   DP+  +KSAI+A  ++  + FK   SL  
Sbjct: 93  KIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKT 152

Query: 102 EAGNNSFMKMSVEDCKDLLQFAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQ 161
           E   N   K ++  CK L+  A EEL  S   + DS+  +  + V D+++WLSAV+SYQ+
Sbjct: 153 E---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSWLSAVMSYQE 212

Query: 162 SCLDGLGEANPKLKETMEGGIDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLL- 221
           +C+DG  E   KLK  +    + ++ LTSN+LA++  +       G    V     RLL 
Sbjct: 213 TCVDGFEEG--KLKTEIRKNFNSSQVLTSNSLAMIKSLD------GYLSSVPKVKTRLLL 272

Query: 222 --GNTEVGSDGFPTWLSGADRRLLAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAP 281
              ++   +D   +WLS  +RR+L A        + PNA VAKDGSG + TI AAL A P
Sbjct: 273 EARSSAKETDHITSWLSNKERRMLKAVDVKA---LKPNATVAKDGSGNFTTINAALKAMP 332

Query: 282 KNSKTRHVIYVKAGIYNEFIIVTKDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFS 341
              + R+ IY+K GIY+E +I+ K   N+ M GDG +KTIVTG KS    I T  TATF 
Sbjct: 333 AKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLTATFV 392

Query: 342 AVGNGFLCKAMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNC 401
           A G GF+ ++MGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY + +RQ+YR+C
Sbjct: 393 AQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQYYRSC 452

Query: 402 VISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQ 461
           VI GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR +  + +G VIHNC + P +
Sbjct: 453 VIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTVAPNE 512

Query: 462 KLFADRFKIPTFLGRPWKEYALTVIMETTLGDFIQPAGYMPWQGT-FALDTCQYLEYANR 521
            L   + +  ++LGRPWK ++ TV+ME+T+ D I P G++ WQ T FA+DT  Y EY N 
Sbjct: 513 DLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAEYKND 572

Query: 522 GPGANTARRVKWKGVRVMGRNEALKFTAGSPFLLGKEWLPATGGNFLLGL 567
           GP   TA RVKW G RV+ + EA+KFT G PFL G EW+ A G    LGL
Sbjct: 573 GPSGATAARVKWPGFRVLNKEEAMKFTVG-PFLQG-EWIQAIGSPVKLGL 606

[Close]

BLAST of MS027560 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 428.3 bits (1100), Expect = 9.5e-120
Identity = 236/527 (44.78%), Postives = 331/527 (62.81%), Query Frame = 0


Query: 42  KAVATICSHTDYQEECQNTLGSIAHNS-SSDDPKEYIKSAIMATVEEFKKGFKLTDSLMV 101
           K + T+CS T Y + C+ TL +      + D+P  ++KSAI A  E+     +   SL  
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKT 166

Query: 102 EAGNNSFMKMSVEDCKDLLQFAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQ 161
           E   N   K ++E CK L++ A EE  AS + +  ++  S E+ V D+ +WLSAV+SYQ+
Sbjct: 167 E---NQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 162 SCLDGLGEANPKLKETMEGGIDIARKLTSNALAIVADVSQVLGDYGLQLKVQPTGRRLLG 221
           +CLDG  E N  LK  ++  ++ ++ LTSN+LA++   ++      L   ++   R LL 
Sbjct: 227 TCLDGFEEGN--LKSEVKTSVNSSQVLTSNSLALIKTFTE-----NLSPVMKVVERHLL- 286

Query: 222 NTEVGSDGFPTWLSGADRRLLAAAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNS 281
                 D  P+W+S  DRR+L A        + PNA VAKDGSG + TI  AL A P+  
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVDVKA---LKPNATVAKDGSGDFTTINDALRAMPEKY 346

Query: 282 KTRHVIYVKAGIYNEFIIVTKDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVG 341
           + R++IYVK GIY+E++ V K   N+ M GDG +KTIVTG KS    I T  TATF A G
Sbjct: 347 EGRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQG 406

Query: 342 NGFLCKAMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVIS 401
            GF+ ++MGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY + +RQ+YR+CVI 
Sbjct: 407 EGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIV 466

Query: 402 GTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLF 461
           GT+DFIFGD+  I QN  I +R+ +  Q+NTVTA GR +  + +G V+HNC+I   + L 
Sbjct: 467 GTIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLK 526

Query: 462 ADRFKIPTFLGRPWKEYALTVIMETTLGDFIQPAGYMPWQGT-FALDTCQYLEYANRGPG 521
             + +  ++LGRPWK Y+ T+IME+ + + I P G++ WQ T FA+DT  Y EY N+G  
Sbjct: 527 PVKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSS 586

Query: 522 ANTARRVKWKGVRVMGRNEALKFTAGSPFLLGKEWLPATGGNFLLGL 567
            +T  RVKW G +V+ + EAL +T G PFL G +W+ A+G    LGL
Sbjct: 587 GDTTSRVKWPGFKVINKEEALNYTVG-PFLQG-DWISASGSPVKLGL 611

[Close]

BLAST of MS027560 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 428.3 bits (1100), Expect = 9.5e-120
Identity = 232/555 (41.80%), Postives = 336/555 (60.54%), Query Frame = 0


Query: 8   VLSLILVVGVALAVVAVVNRNHG-SFDGENLSPKMKAVATICSHTDYQEECQNTLGSIAH 67
           + +L++++ VA+ +    N +H        +     AV  +C+ TDY+E C N+L  +  
Sbjct: 18  ITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNSL--MKA 77

Query: 68  NSSSDDPKEYIKSAIMATVEEFKKGFKLTD-SLMVEAGNNSFMKMSVEDCKDLLQFAVEE 127
           +  S  P + IK     T+   +   K     L  +A N+   K ++E C+ L+  A ++
Sbjct: 78  SPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLMNDATDD 137

Query: 128 LQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETMEGGIDIAR 187
           L+    +         E+ V D+  WLS  I+YQQ+C+D   E N KL + M+     +R
Sbjct: 138 LKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFKTSR 197

Query: 188 KLTSNALAIVADVSQVLGDY---GLQLKVQPTGRRLLGNTEVGSDGFPTWLSGADRRLLA 247
           +LTSN LA++ ++S +LG++   G+   +    R+LL       DG P+W+ G + R L 
Sbjct: 198 ELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS----AEDGIPSWV-GPNTRRLM 257

Query: 248 AAKGGGGGKVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNEFIIVTKD 307
           A KGG    V  N VVA DGSGQYKTI  AL A PK ++   VIY+K G+YNE + VTK 
Sbjct: 258 ATKGG----VKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKK 317

Query: 308 YTNIFMYGDGPRKTIVTGRKSFRDG-ITTMNTATFSAVGNGFLCKAMGFQNTAGPEGHQA 367
            T++   GDGP KT +TG  ++  G + T  TAT +  G+ F  K +GF+NTAGPEGHQA
Sbjct: 318 MTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQA 377

Query: 368 VALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 427
           VALRV +D + F+NC++DGYQDTLYVH++RQF+R+C +SGTVDFIFGD   ++QN  I+V
Sbjct: 378 VALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVV 437

Query: 428 RRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKEYALTV 487
           R+PM +Q   +TA GR + +E +GLV+ NC I  E      +     +LGRPWKE++ T+
Sbjct: 438 RKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTI 497

Query: 488 IMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMGRNEALK 547
           IM TT+ D I PAG++PW G FAL+T  Y EY N GPG+N A+RVKW G++ +   +AL+
Sbjct: 498 IMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALR 557

Query: 548 FTAGSPFLLGKEWLP 557
           FT  + FL G  W+P
Sbjct: 558 FTP-ARFLRGNLWIP 560

The following BLAST results are available for this feature:

BLAST of MS027560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146333.1	0.0e+00	100.00	pectinesterase-like [Momordica charantia]	[more]
XP_022146322.1	1.2e-262	81.68	pectinesterase-like [Momordica charantia]	[more]
XP_008466382.2	2.6e-252	77.74	PREDICTED: pectinesterase-like [Cucumis melo]	[more]
KAA0050276.1	5.7e-252	76.58	pectinesterase-like [Cucumis melo var. makuwa] >TYJ98131.1 pectinesterase-like [...	[more]
KGN49779.1	2.4e-250	76.94	hypothetical protein Csa_017783 [Cucumis sativus]	[more]

BLAST of MS027560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3E8Z8	9.5e-125	41.49	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q8GX86	1.2e-124	41.06	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q9SMY6	7.5e-122	46.04	Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3...	[more]
Q7Y201	1.3e-118	44.78	Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3...	[more]
Q9FJ21	1.3e-118	41.80	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of MS027560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CYB2	0.0e+00	100.00	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015570 PE=3 SV=1	[more]
A0A6J1CZA2	5.9e-263	81.68	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015559 PE=3 SV=1	[more]
A0A1S3CR40	1.2e-252	77.74	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503798 PE=3 SV=1	[more]
A0A5A7U7W4	2.8e-252	76.58	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090...	[more]
A0A0A0KLN2	1.2e-250	76.94	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1	[more]

BLAST of MS027560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G27870.1	6.8e-126	41.49	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G05610.1	8.8e-126	41.06	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G33230.1	5.4e-123	46.04	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G26450.1	9.5e-120	44.78	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	9.5e-120	41.80	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 39..194 e-value: 5.0E-36 score: 135.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 43..194 e-value: 1.5E-25 score: 90.3
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 17..196 e-value: 7.7E-21 score: 72.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 40..198 e-value: 6.2E-31 score: 109.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 42..196
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 249..566 e-value: 2.1E-108 score: 364.5
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 255..545 e-value: 1.1E-130 score: 434.8
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 7..563
None	No IPR available	PANTHER	PTHR31707:SF164	PECTINESTERASE 4-RELATED	coord: 7..563
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 46..196 e-value: 6.50315E-50 score: 167.615
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 280..299
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 399..408
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 254..565

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS027560.1	MS027560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity