MS027555 (gene) Bitter gourd (TR) v1

BLAST of MS027555 vs. NCBI nr
Match: XP_022146381.1 (ubiquitin carboxyl-terminal hydrolase 13-like [Momordica charantia])

HSP 1 Score: 512.7 bits (1319), Expect = 2.2e-141
Identity = 261/301 (86.71%), Postives = 266/301 (88.37%), Query Frame = 0

Query: 1   MQRNNTISPSSEAADSVIWLIIFCSLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYE 60
           MQRNNTISPSSEAADSV        LSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYE
Sbjct: 1   MQRNNTISPSSEAADSV--------LSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYE 60

Query: 61  SQIFNAGGYKWKLAVYPNGDVKRNGSDHISLYLVKAEDDIPTSEVNVVFTFLVYDNLNDK 120
           SQIFNAGGYKWKLAVYPNGDVKRNGSDHISLYLVKAED IPTSEVNVVFTFLVYDNLNDK
Sbjct: 61  SQIFNAGGYKWKLAVYPNGDVKRNGSDHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDK 120

Query: 121 YLAVQDEKVRRFHSIKTEWGFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEV 180
           YLAVQDEKVRRFHSI+TEW FEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEV
Sbjct: 121 YLAVQDEKVRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEV 180

Query: 181 VSFIKEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARFGFLSLKL 240
           VSFIKEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYR    L P+G             
Sbjct: 181 VSFIKEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRCFKEL-PQGSQ----------- 240

Query: 241 MFDSFKELPQGKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 300
           ++  F+     KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL
Sbjct: 241 VYTEFEIAVLSKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 281

Query: 301 F 302
           F
Sbjct: 301 F 281

BLAST of MS027555 vs. NCBI nr
Match: XP_022146382.1 (uncharacterized protein LOC111015610 [Momordica charantia])

HSP 1 Score: 420.6 bits (1080), Expect = 1.1e-113
Identity = 217/312 (69.55%), Postives = 240/312 (76.92%), Query Frame = 0

Query: 1   MQRNNTISPSSEAADSVIWLIIFCSLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYE 60
           MQRN+ ISPSS+A    +       L   RDEKPLHYTLQIQ FSLLKTALA S+ DRYE
Sbjct: 36  MQRNHIISPSSQADSGSV-------LRSIRDEKPLHYTLQIQPFSLLKTALATSSRDRYE 95

Query: 61  SQIFNAGGYKWKLAVYPNGDVKRNGSDHISLYLVKAEDDIPTSEVNVVFTFLVYDNLNDK 120
           S IF+  G+KWKLAVYP+GDVKRNGS+HISLYLV AEDDIPT ++ VVFT LVYDNL D 
Sbjct: 96  SPIFSVAGFKWKLAVYPSGDVKRNGSNHISLYLVTAEDDIPTLDLTVVFTILVYDNLRDN 155

Query: 121 YLAVQDEKVRRFHSIKTEWGFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEV 180
           YLAV D KVRRFH++K EWGFEKL+SLETFNDSSNGFLV DCCAFGVDI V KCDGKGE 
Sbjct: 156 YLAVLDGKVRRFHAMKKEWGFEKLVSLETFNDSSNGFLVDDCCAFGVDIFVMKCDGKGEA 215

Query: 181 VSFIKEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARFGFLSLKL 240
           +S IK+PKNN+YTWKTNNFSQLD+NM             K+ L+PKG  +A   +LSL L
Sbjct: 216 LSLIKQPKNNKYTWKTNNFSQLDENM-------------KMSLYPKGSPKASPDYLSLFL 275

Query: 241 MFDSFKELPQG-------------KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLI 300
             +S KELPQG             KYWFEFGDLGLGF +FMSLRDLNEVTKGYICDDTLI
Sbjct: 276 RCESLKELPQGCRVYAEFEIAVLSKYWFEFGDLGLGFPDFMSLRDLNEVTKGYICDDTLI 327

BLAST of MS027555 vs. NCBI nr
Match: XP_022146380.1 (probable inactive serine/threonine-protein kinase fnkC [Momordica charantia])

HSP 1 Score: 392.9 bits (1008), Expect = 2.5e-105
Identity = 199/287 (69.34%), Postives = 224/287 (78.05%), Query Frame = 0

Query: 29  TRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRNGSDH 88
           TRDEKPLHYTLQIQSFSLLK ALA+SN DRYESQIFNAGGYKWKLAVYPNGDVKRNG+DH
Sbjct: 12  TRDEKPLHYTLQIQSFSLLKAALASSNRDRYESQIFNAGGYKWKLAVYPNGDVKRNGADH 71

Query: 89  ISLYLVKAEDDI-PTSEVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIKTEWGFEKLISL 148
           ISLYLVKA+DDI P S                        KVRRFH++K EWGFEKLISL
Sbjct: 72  ISLYLVKADDDINPCSH----------------------GKVRRFHAMKAEWGFEKLISL 131

Query: 149 ETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLDKNMY 208
           +TFNDSSNGF+VGDCC FGVD+VV KCDGKGEVVSFIKEPK+++YTWKT NFSQLDK  Y
Sbjct: 132 DTFNDSSNGFVVGDCCGFGVDVVVMKCDGKGEVVSFIKEPKSHKYTWKTKNFSQLDKKFY 191

Query: 209 ESDPFTVEGYRWKIRLHPKGCSQARFGFLSLKLMFDSFKELPQGKY-----------WFE 268
           ESDPFTVEGYRWK+ L+PKG S+A+ GFLSL L ++S++E+PQG             WF 
Sbjct: 192 ESDPFTVEGYRWKLWLYPKGSSKAKTGFLSLYLSYNSYEEIPQGSQVYVEYEDSHNCWFG 251

Query: 269 FGDL----GLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLK 300
           FG +    G G  NF+SLRDLNE T+G+I DDT+IVEVKINVVSTLK
Sbjct: 252 FGGVESGRGWGHPNFLSLRDLNEPTEGFIVDDTIIVEVKINVVSTLK 276

BLAST of MS027555 vs. NCBI nr
Match: XP_038874970.1 (uncharacterized protein LOC120067490 [Benincasa hispida])

HSP 1 Score: 365.2 bits (936), Expect = 5.6e-97
Identity = 183/301 (60.80%), Postives = 224/301 (74.42%), Query Frame = 0

Query: 29  TRDEKPLHYTLQIQSFSLLKTALAASNC-DRYESQIFNAGGYKWKLAVYPNGDVKRNGSD 88
           +RDEKPLHYTL+IQSFSLLK A+AAS+  +RYESQIF AGGYKWKLA+YPNGD KRN ++
Sbjct: 30  SRDEKPLHYTLKIQSFSLLKAAVAASSIRNRYESQIFKAGGYKWKLALYPNGDFKRNVAN 89

Query: 89  HISLYLVKAEDDIPTS--EVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIKTEWGFEKLI 148
           HISLYLV AED+I +S  E+ V+  FLVYDNL D+Y  +QD KVR FH+IK EWGFEKL+
Sbjct: 90  HISLYLVSAEDEISSSGLEIKVMIKFLVYDNLKDRYWTIQDGKVRCFHAIKKEWGFEKLV 149

Query: 149 SLETFNDSSNGFLVGDCCAFGVDIVV-KKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLDK 208
           SL+TFND+SNG LV DCCAFGVD++V K  DG  E++S IK+ KN +YTWK NNFSQL K
Sbjct: 150 SLDTFNDTSNGLLVDDCCAFGVDVIVMKSYDGNNEILSLIKQLKNYKYTWKINNFSQLSK 209

Query: 209 NMYESDPFTVEGYRWKIRLHPKGCSQARFGFLSLKLMFDSFKELPQG------------- 268
           N+YESDPFTV+ Y WKI L+P+G S A  G++S+ L FDSF++LPQG             
Sbjct: 210 NVYESDPFTVDNYTWKIELYPRGDSLAMTGYMSMFLRFDSFEKLPQGSRVYVEYDIAVLS 269

Query: 269 -----------KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 302
                      K+WF+      GF NFMSL ++ E  KG++CDDTL+VEVKINV+S LK 
Sbjct: 270 QLGGAYTNYPVKHWFDGETKSWGFGNFMSLSNVKEPMKGFLCDDTLMVEVKINVISILKS 329

BLAST of MS027555 vs. NCBI nr
Match: XP_038875772.1 (uncharacterized protein LOC120068142 [Benincasa hispida])

HSP 1 Score: 357.8 bits (917), Expect = 8.9e-95
Identity = 190/327 (58.10%), Postives = 224/327 (68.50%), Query Frame = 0

Query: 4   NNTISPSSEAADSVIWLIIFCSLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQI 63
           NNT+SP+    +S         +  TR EKP+HYTL+IQSFSLLK  LA+S   R+ESQ 
Sbjct: 10  NNTVSPNPHGDNS------SSVVMSTRHEKPVHYTLKIQSFSLLKNGLASSQRQRFESQK 69

Query: 64  FNAGGYKWKLAVYPNGDVKRNGSDHISLYLVKAEDDI--PTSEVNVVFTFLVYDNLNDKY 123
           FNAG Y+WKLA+YPNGD +RN S+HISLYLV   ++I   TSEVNVVFTFLVYD L  KY
Sbjct: 70  FNAGCYEWKLALYPNGDERRNVSNHISLYLVIVGNNILSTTSEVNVVFTFLVYDTLRGKY 129

Query: 124 LAVQDEKVRRFHSIKTEWGFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCD-GKGEV 183
           L VQD K+RRF ++KTEWG EKL+ L+TFND+SNGFLV DCC FGVDI V  CD G GEV
Sbjct: 130 LTVQDGKMRRFSAMKTEWGIEKLLPLDTFNDASNGFLVDDCCVFGVDIFVMSCDGGMGEV 189

Query: 184 VSFIKEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARFGFLSLKL 243
            S IK+PKN EYTWK NNFSQL   + ES+PFTVE YRWKI L+P GCS+A+ GFLS+ L
Sbjct: 190 FSLIKQPKNYEYTWKLNNFSQLGSTLKESNPFTVESYRWKILLYPSGCSKAKSGFLSMYL 249

Query: 244 MFDSFKELPQGKY------------------------WFEFGDL----GLGFRNFMSLRD 300
           +FDS  +LPQG                          WF F  +    G G  NF SL D
Sbjct: 250 LFDSSNQLPQGSQVYVEYEMAILSQLEAVPVKETYNCWFGFDGVEAGRGWGAPNFSSLSD 309

BLAST of MS027555 vs. ExPASy Swiss-Prot
Match: Q9FPT1 (Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 5.0e-08
Identity = 39/141 (27.66%), Postives = 62/141 (43.97%), Query Frame = 0

Query: 185 KEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARFGFLSLKLMFDS 244
           ++P   ++TW   NFS+ +   + SD F V GY+W+I + PKG        LS+ L    
Sbjct: 50  EDPPTLKFTWTIPNFSRQNTRKHYSDVFVVGGYKWRILIFPKG---NNVDHLSMYLDVSD 109

Query: 245 FKELPQGKYWFEFGDLGL---------------------------GFRNFMSLRDLNEVT 299
              LP G  W  +    L                           GF +FM L +L + +
Sbjct: 110 AASLPYG--WSRYAQFSLAVVNQIHTRYTVRKETQHQFNARESDWGFTSFMPLSELYDPS 169

BLAST of MS027555 vs. ExPASy Swiss-Prot
Match: Q84WU2 (Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 5.0e-08
Identity = 39/141 (27.66%), Postives = 63/141 (44.68%), Query Frame = 0

Query: 185 KEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARFGFLSLKLMFDS 244
           ++P + ++TW    F++L+   + SD F V GY+W+I + PKG        LS+ L    
Sbjct: 49  EDPPSLKFTWTIPMFTRLNTRKHYSDVFVVGGYKWRILIFPKG---NNVDHLSMYLDVAD 108

Query: 245 FKELPQGKYWFEFGDLGL---------------------------GFRNFMSLRDLNEVT 299
              LP G  W  +    L                           GF +FM L +L E T
Sbjct: 109 AANLPYG--WSRYSQFSLAVVNQVNNRYSIRKETQHQFNARESDWGFTSFMPLSELYEPT 168

BLAST of MS027555 vs. ExPASy Swiss-Prot
Match: Q9M2I9 (MATH domain and coiled-coil domain-containing protein At3g58270 OS=Arabidopsis thaliana OX=3702 GN=At3g58270 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 8.6e-08
Identity = 46/147 (31.29%), Postives = 72/147 (48.98%), Query Frame = 0

Query: 61  SQIFNAGGYKWKLAVYPNGDVKRNGSDHISLYL-VKAEDDIPTS-EVNVVFTFLVYDNLN 120
           S  F   G KW+L  +P G    NG + +SLYL V   + +P     +  F F V + L+
Sbjct: 26  SDEFFVDGCKWRLLAFPKG----NGVEKLSLYLAVAGSEFLPDGWRRHAYFHFSVVNQLS 85

Query: 121 DKYLAVQDEKVRRFHSIKTEWGFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKG 180
           D+    ++ K   F +  ++WGF  ++SL+  +D   GFLV       VD+ V +  GK 
Sbjct: 86  DELSQARETK-NWFDASTSDWGFTSMLSLKKLHDKDGGFLVNGELKIVVDVSVLEVIGKL 145

Query: 181 EVVSFIKEPKNNEYTWKTNNFSQLDKN 206
           +V      P  +E T  T   S+L++N
Sbjct: 146 DV------PVESEET-TTKALSELEEN 160

BLAST of MS027555 vs. ExPASy Swiss-Prot
Match: Q9M2H9 (MATH domain and coiled-coil domain-containing protein At3g58370 OS=Arabidopsis thaliana OX=3702 GN=At3g58370 PE=4 SV=3)

HSP 1 Score: 59.3 bits (142), Expect = 8.6e-08
Identity = 35/131 (26.72%), Postives = 59/131 (45.04%), Query Frame = 0

Query: 186 EPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKG------------------ 245
           E  +N++TW   NFS L  +   SDPF + G +W +  +PKG                  
Sbjct: 4   EEVDNKFTWVIKNFSTLQYDKIYSDPFVIGGCKWHLLAYPKGNKFNNSLSLYLVVDDARA 63

Query: 246 --CSQARFGFLSLKL---MFDSFKELPQGKYWFEFGDLGLGFRNFMSLRDLNEVTKGYIC 294
             C   R+   SL +   + D   +  + + WF   +LG GF + + L +L+    GY+ 
Sbjct: 64  LPCGWRRYAQFSLTIINQLTDKLSQRGEKQNWFNQRNLGSGFTSMIPLPNLHAKNAGYLV 123

BLAST of MS027555 vs. ExPASy Swiss-Prot
Match: Q9M2J5 (MATH domain and coiled-coil domain-containing protein At3g58210 OS=Arabidopsis thaliana OX=3702 GN=At3g58210 PE=4 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 2.1e-06
Identity = 42/145 (28.97%), Postives = 65/145 (44.83%), Query Frame = 0

Query: 37  YTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRNGSDHISLYLVKA 96
           +T  IQ+FS  ++ +  SN        F  GG KW+L VYP G  K    DH+SL+L  A
Sbjct: 9   FTWVIQNFSSSQSRVVPSN-------QFVIGGCKWRLLVYPEGFNK--SGDHLSLFLEVA 68

Query: 97  ED-DIPTSEVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIKTEWGFEKLISLETFNDSSN 156
           +   +P          L   N +   ++ ++E  + F+     WG   +I L   +    
Sbjct: 69  DPRSLPPGWSRHARYLLTIVNQHSDKISKRNEATKWFNQKIPGWGLSAMIPLTKLHAKDG 128

Query: 157 GFLVGDCCAFGVDIVVKKCDGKGEV 181
           GFLV D      ++ V +  GK +V
Sbjct: 129 GFLVNDELKIVAEVNVLEVIGKLDV 144

BLAST of MS027555 vs. ExPASy TrEMBL
Match: A0A6J1CZ69 (ubiquitin carboxyl-terminal hydrolase 13-like OS=Momordica charantia OX=3673 GN=LOC111015609 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.0e-141
Identity = 261/301 (86.71%), Postives = 266/301 (88.37%), Query Frame = 0

Query: 1   MQRNNTISPSSEAADSVIWLIIFCSLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYE 60
           MQRNNTISPSSEAADSV        LSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYE
Sbjct: 1   MQRNNTISPSSEAADSV--------LSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYE 60

Query: 61  SQIFNAGGYKWKLAVYPNGDVKRNGSDHISLYLVKAEDDIPTSEVNVVFTFLVYDNLNDK 120
           SQIFNAGGYKWKLAVYPNGDVKRNGSDHISLYLVKAED IPTSEVNVVFTFLVYDNLNDK
Sbjct: 61  SQIFNAGGYKWKLAVYPNGDVKRNGSDHISLYLVKAEDGIPTSEVNVVFTFLVYDNLNDK 120

Query: 121 YLAVQDEKVRRFHSIKTEWGFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEV 180
           YLAVQDEKVRRFHSI+TEW FEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEV
Sbjct: 121 YLAVQDEKVRRFHSIQTEWEFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEV 180

Query: 181 VSFIKEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARFGFLSLKL 240
           VSFIKEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYR    L P+G             
Sbjct: 181 VSFIKEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRCFKEL-PQGSQ----------- 240

Query: 241 MFDSFKELPQGKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 300
           ++  F+     KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL
Sbjct: 241 VYTEFEIAVLSKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 281

Query: 301 F 302
           F
Sbjct: 301 F 281

BLAST of MS027555 vs. ExPASy TrEMBL
Match: A0A6J1CZF7 (uncharacterized protein LOC111015610 OS=Momordica charantia OX=3673 GN=LOC111015610 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.4e-114
Identity = 217/312 (69.55%), Postives = 240/312 (76.92%), Query Frame = 0

Query: 1   MQRNNTISPSSEAADSVIWLIIFCSLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYE 60
           MQRN+ ISPSS+A    +       L   RDEKPLHYTLQIQ FSLLKTALA S+ DRYE
Sbjct: 36  MQRNHIISPSSQADSGSV-------LRSIRDEKPLHYTLQIQPFSLLKTALATSSRDRYE 95

Query: 61  SQIFNAGGYKWKLAVYPNGDVKRNGSDHISLYLVKAEDDIPTSEVNVVFTFLVYDNLNDK 120
           S IF+  G+KWKLAVYP+GDVKRNGS+HISLYLV AEDDIPT ++ VVFT LVYDNL D 
Sbjct: 96  SPIFSVAGFKWKLAVYPSGDVKRNGSNHISLYLVTAEDDIPTLDLTVVFTILVYDNLRDN 155

Query: 121 YLAVQDEKVRRFHSIKTEWGFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEV 180
           YLAV D KVRRFH++K EWGFEKL+SLETFNDSSNGFLV DCCAFGVDI V KCDGKGE 
Sbjct: 156 YLAVLDGKVRRFHAMKKEWGFEKLVSLETFNDSSNGFLVDDCCAFGVDIFVMKCDGKGEA 215

Query: 181 VSFIKEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARFGFLSLKL 240
           +S IK+PKNN+YTWKTNNFSQLD+NM             K+ L+PKG  +A   +LSL L
Sbjct: 216 LSLIKQPKNNKYTWKTNNFSQLDENM-------------KMSLYPKGSPKASPDYLSLFL 275

Query: 241 MFDSFKELPQG-------------KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLI 300
             +S KELPQG             KYWFEFGDLGLGF +FMSLRDLNEVTKGYICDDTLI
Sbjct: 276 RCESLKELPQGCRVYAEFEIAVLSKYWFEFGDLGLGFPDFMSLRDLNEVTKGYICDDTLI 327

BLAST of MS027555 vs. ExPASy TrEMBL
Match: A0A6J1CX47 (probable inactive serine/threonine-protein kinase fnkC OS=Momordica charantia OX=3673 GN=LOC111015608 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 1.2e-105
Identity = 199/287 (69.34%), Postives = 224/287 (78.05%), Query Frame = 0

Query: 29  TRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRNGSDH 88
           TRDEKPLHYTLQIQSFSLLK ALA+SN DRYESQIFNAGGYKWKLAVYPNGDVKRNG+DH
Sbjct: 12  TRDEKPLHYTLQIQSFSLLKAALASSNRDRYESQIFNAGGYKWKLAVYPNGDVKRNGADH 71

Query: 89  ISLYLVKAEDDI-PTSEVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIKTEWGFEKLISL 148
           ISLYLVKA+DDI P S                        KVRRFH++K EWGFEKLISL
Sbjct: 72  ISLYLVKADDDINPCSH----------------------GKVRRFHAMKAEWGFEKLISL 131

Query: 149 ETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLDKNMY 208
           +TFNDSSNGF+VGDCC FGVD+VV KCDGKGEVVSFIKEPK+++YTWKT NFSQLDK  Y
Sbjct: 132 DTFNDSSNGFVVGDCCGFGVDVVVMKCDGKGEVVSFIKEPKSHKYTWKTKNFSQLDKKFY 191

Query: 209 ESDPFTVEGYRWKIRLHPKGCSQARFGFLSLKLMFDSFKELPQGKY-----------WFE 268
           ESDPFTVEGYRWK+ L+PKG S+A+ GFLSL L ++S++E+PQG             WF 
Sbjct: 192 ESDPFTVEGYRWKLWLYPKGSSKAKTGFLSLYLSYNSYEEIPQGSQVYVEYEDSHNCWFG 251

Query: 269 FGDL----GLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLK 300
           FG +    G G  NF+SLRDLNE T+G+I DDT+IVEVKINVVSTLK
Sbjct: 252 FGGVESGRGWGHPNFLSLRDLNEPTEGFIVDDTIIVEVKINVVSTLK 276

BLAST of MS027555 vs. ExPASy TrEMBL
Match: A0A6J1CXZ0 (ubiquitin carboxyl-terminal hydrolase 12-like OS=Momordica charantia OX=3673 GN=LOC111015607 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 1.1e-87
Identity = 190/327 (58.10%), Postives = 212/327 (64.83%), Query Frame = 0

Query: 1   MQRNNTISPS-SEAADSVIWLIIFCSLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRY 60
           M+R N I  S S  AD+    ++  S   TRDEKPLHYTLQIQSFSLLKTAL  S+CDRY
Sbjct: 1   MERMNIIFHSLSSEADTDSGCVVIRS---TRDEKPLHYTLQIQSFSLLKTALVRSDCDRY 60

Query: 61  ESQIFNAGGYKWKLAVYPNGDVKRNGS-DHISLYLVKAEDDIPTSEVNVVFTFLVYDNLN 120
           ES+IFNAGGYKWKLA+YP GDVKRNGS DHISLYLVKAEDDIPTSEVNVVFTFLVYD+L 
Sbjct: 61  ESRIFNAGGYKWKLAMYPTGDVKRNGSHDHISLYLVKAEDDIPTSEVNVVFTFLVYDSLK 120

Query: 121 DKYLAVQDEKVRRFHSIKTEWGFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKG 180
           DKYLAVQD  VRRFH+IKTEWGFEKLISL+TFNDSSNGFLV DCCAFGVD+VV K     
Sbjct: 121 DKYLAVQDGIVRRFHAIKTEWGFEKLISLDTFNDSSNGFLVDDCCAFGVDVVVMK----- 180

Query: 181 EVVSFIKEPKNNEYTWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARFGFLSL 240
                                              +EGYRWK+ L+PKGCS A  GFLSL
Sbjct: 181 -----------------------------------LEGYRWKLWLYPKGCSNAETGFLSL 240

Query: 241 KLMFDSFKELPQG------------------------KYWFEFGDLGLGFRNFMSLRDLN 300
            L+ DS KE+ QG                         YWF  G       NF+SL +L 
Sbjct: 241 YLILDSSKEILQGSKVYVEYELALLSQLGAEPEKETYNYWFAGGG-----PNFISLSELK 279

Query: 301 EVTKGYICDDTLIVEVKINVVSTLKLF 302
           +  KGY+ +DTL VEVKINV+ST K F
Sbjct: 301 KPLKGYLHNDTLKVEVKINVISTSKSF 279

BLAST of MS027555 vs. ExPASy TrEMBL
Match: A0A6J1CYF6 (uncharacterized protein LOC111015611 OS=Momordica charantia OX=3673 GN=LOC111015611 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 3.3e-87
Identity = 187/314 (59.55%), Postives = 203/314 (64.65%), Query Frame = 0

Query: 15  DSVIWLIIFCSLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLA 74
           D++   I+   L   RDEKPLHYTLQIQ FSLLKTALA  + +RYES IFN GG+KWKLA
Sbjct: 304 DTLSGYILNDILRSIRDEKPLHYTLQIQPFSLLKTALATCSRERYESPIFNVGGFKWKLA 363

Query: 75  VYPNGDVKRNGSDHISLYLVKAEDDI--PTSEVNVVFTFLVYDNLNDKYLAVQDEKVRRF 134
           +YPNGD  RNGS +ISLYLV AED+I   TSEVNVV TFLVYDNL D YLAV D KVRRF
Sbjct: 364 LYPNGDKNRNGSGYISLYLVTAEDEIVSATSEVNVVLTFLVYDNLRDNYLAVLDGKVRRF 423

Query: 135 HSIKTEWGFEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEY 194
           H++KTEWGF KL+SLETFNDSSNGFLV DCCAFGVDI V K                   
Sbjct: 424 HAMKTEWGFGKLVSLETFNDSSNGFLVDDCCAFGVDIFVMK------------------- 483

Query: 195 TWKTNNFSQLDKNMYESDPFTVEGYRWKIRLHPKGCSQARFGFLSLKLMFDSFKE-LPQG 254
                                +E YRWKIRLHPKGCSQ   GFLSL L+FDSFKE LPQG
Sbjct: 484 ---------------------LESYRWKIRLHPKGCSQESAGFLSLYLIFDSFKELLPQG 543

Query: 255 ------------------------KYWFEFG--DLGLGFRNFMSLRDLNEVTKGYICDDT 300
                                   K WFEFG  + GLGF  FM LRDL EV KGYICDDT
Sbjct: 544 CRVYAEFEIAVLSKYGVGHKKLESKCWFEFGGIETGLGFPEFMPLRDLKEVKKGYICDDT 577

BLAST of MS027555 vs. TAIR 10
Match: AT3G17380.1 (TRAF-like family protein )

HSP 1 Score: 188.3 bits (477), Expect = 8.7e-48
Identity = 101/233 (43.35%), Postives = 140/233 (60.09%), Query Frame = 0

Query: 31  DEKPLHYTLQIQSFSLL-KTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRNGSDHI 90
           D  P HY ++I+SFSLL K A+     +RYE++ F AGGYKWKL +YPNG+  +N  DH+
Sbjct: 16  DAPPTHYMVKIESFSLLTKHAI-----ERYETESFEAGGYKWKLVLYPNGNKSKNTKDHV 75

Query: 91  SLYLVKAEDD--IPTSEVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIKTEWGFEKLISL 150
           S+YL  A+     P  EV  VF   + D   D YL +Q  + RRFHS+K EWGF+K I  
Sbjct: 76  SVYLSLADSSSLSPGWEVYAVFRLYLLDQNKDNYLILQGNE-RRFHSVKREWGFDKFIPT 135

Query: 151 ETFNDSSNGFLVGDCCAFGVDIVVKK--CDGKGEVVSFIKEPKNNEYTWKTNNFSQLDKN 210
            TF+D+SNG+L+ D C FG D+ V K    G+GE +S IK+  ++++ WK  NFS+LDK 
Sbjct: 136 GTFSDASNGYLMEDTCMFGADVFVSKERRSGRGECLSMIKDATSSKHVWKIENFSKLDKE 195

Query: 211 MYESDPFTVEGYRWKIRLHPKGCSQARFGFLSLKLMFDSFKELPQG-KYWFEF 258
            Y+S+ F     +WKI  +P G  Q     LS+ L     + +  G K + EF
Sbjct: 196 SYDSNAFFAGDRKWKIEFYPTGTKQGTGTHLSIYLTLVDPETISDGTKIFVEF 242

BLAST of MS027555 vs. TAIR 10
Match: AT2G04170.1 (TRAF-like family protein )

HSP 1 Score: 176.8 bits (447), Expect = 2.6e-44
Identity = 102/298 (34.23%), Postives = 156/298 (52.35%), Query Frame = 0

Query: 30  RDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRNGSDHI 89
           RDE+P +  L I SFS++K        + YES +F AGGYKW+L +Y NG+    G++HI
Sbjct: 125 RDERPSNKILTITSFSVIK-----GRGEPYESSVFEAGGYKWRLVLYVNGNQNDGGNNHI 184

Query: 90  SLYL-VKAEDDIPTS-EVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIKTEWGFEKLISL 149
           SLY+ ++  + +P   EVNV     VY+    KYL V+D  V+R++  K EWG+ KLI L
Sbjct: 185 SLYVRIEETESLPKGWEVNVELKLFVYNGKQRKYLIVKDGIVKRYNDAKKEWGYGKLIPL 244

Query: 150 ETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLDKNMY 209
            TF D++ G+L  D  +FG +I         E V+FI  P NN +TWK  +FS L+   Y
Sbjct: 245 TTFLDTNEGYLEQDIASFGAEIFSGTAVQVQEKVTFISNPPNNVFTWKILHFSNLEDKFY 304

Query: 210 ESDPFTVEGYRWKIRLHPKGCSQAR----------------------FGFLSLKL---MF 269
            SD F VE   W++  +PKG    R                      +G ++L+L     
Sbjct: 305 YSDDFLVEDRYWRLGFNPKGTGDGRSQAIPIFLYAQGHKPNAVATNTWGAVNLRLKNQRS 364

Query: 270 DSFKELPQGKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 301
            +  ++    ++    D G+G    +SL + N+ +KGY  +D++I E ++  VS   +
Sbjct: 365 SNHAQIYSAAWYPTRSDYGVGVNTIISLAEFNDASKGYSVNDSIIFEAEMVKVSVTNI 417

BLAST of MS027555 vs. TAIR 10
Match: AT2G04170.2 (TRAF-like family protein )

HSP 1 Score: 176.8 bits (447), Expect = 2.6e-44
Identity = 102/298 (34.23%), Postives = 156/298 (52.35%), Query Frame = 0

Query: 30  RDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRNGSDHI 89
           RDE+P +  L I SFS++K        + YES +F AGGYKW+L +Y NG+    G++HI
Sbjct: 125 RDERPSNKILTITSFSVIK-----GRGEPYESSVFEAGGYKWRLVLYVNGNQNDGGNNHI 184

Query: 90  SLYL-VKAEDDIPTS-EVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIKTEWGFEKLISL 149
           SLY+ ++  + +P   EVNV     VY+    KYL V+D  V+R++  K EWG+ KLI L
Sbjct: 185 SLYVRIEETESLPKGWEVNVELKLFVYNGKQRKYLIVKDGIVKRYNDAKKEWGYGKLIPL 244

Query: 150 ETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLDKNMY 209
            TF D++ G+L  D  +FG +I         E V+FI  P NN +TWK  +FS L+   Y
Sbjct: 245 TTFLDTNEGYLEQDIASFGAEIFSGTAVQVQEKVTFISNPPNNVFTWKILHFSNLEDKFY 304

Query: 210 ESDPFTVEGYRWKIRLHPKGCSQAR----------------------FGFLSLKL---MF 269
            SD F VE   W++  +PKG    R                      +G ++L+L     
Sbjct: 305 YSDDFLVEDRYWRLGFNPKGTGDGRSQAIPIFLYAQGHKPNAVATNTWGAVNLRLKNQRS 364

Query: 270 DSFKELPQGKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 301
            +  ++    ++    D G+G    +SL + N+ +KGY  +D++I E ++  VS   +
Sbjct: 365 SNHAQIYSAAWYPTRSDYGVGVNTIISLAEFNDASKGYSVNDSIIFEAEMVKVSVTNI 417

BLAST of MS027555 vs. TAIR 10
Match: AT2G04170.3 (TRAF-like family protein )

HSP 1 Score: 176.8 bits (447), Expect = 2.6e-44
Identity = 102/298 (34.23%), Postives = 156/298 (52.35%), Query Frame = 0

Query: 30  RDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRNGSDHI 89
           RDE+P +  L I SFS++K        + YES +F AGGYKW+L +Y NG+    G++HI
Sbjct: 3   RDERPSNKILTITSFSVIK-----GRGEPYESSVFEAGGYKWRLVLYVNGNQNDGGNNHI 62

Query: 90  SLYL-VKAEDDIPTS-EVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIKTEWGFEKLISL 149
           SLY+ ++  + +P   EVNV     VY+    KYL V+D  V+R++  K EWG+ KLI L
Sbjct: 63  SLYVRIEETESLPKGWEVNVELKLFVYNGKQRKYLIVKDGIVKRYNDAKKEWGYGKLIPL 122

Query: 150 ETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLDKNMY 209
            TF D++ G+L  D  +FG +I         E V+FI  P NN +TWK  +FS L+   Y
Sbjct: 123 TTFLDTNEGYLEQDIASFGAEIFSGTAVQVQEKVTFISNPPNNVFTWKILHFSNLEDKFY 182

Query: 210 ESDPFTVEGYRWKIRLHPKGCSQAR----------------------FGFLSLKL---MF 269
            SD F VE   W++  +PKG    R                      +G ++L+L     
Sbjct: 183 YSDDFLVEDRYWRLGFNPKGTGDGRSQAIPIFLYAQGHKPNAVATNTWGAVNLRLKNQRS 242

Query: 270 DSFKELPQGKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 301
            +  ++    ++    D G+G    +SL + N+ +KGY  +D++I E ++  VS   +
Sbjct: 243 SNHAQIYSAAWYPTRSDYGVGVNTIISLAEFNDASKGYSVNDSIIFEAEMVKVSVTNI 295

BLAST of MS027555 vs. TAIR 10
Match: AT2G04170.4 (TRAF-like family protein )

HSP 1 Score: 176.8 bits (447), Expect = 2.6e-44
Identity = 102/298 (34.23%), Postives = 156/298 (52.35%), Query Frame = 0

Query: 30  RDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKRNGSDHI 89
           RDE+P +  L I SFS++K        + YES +F AGGYKW+L +Y NG+    G++HI
Sbjct: 3   RDERPSNKILTITSFSVIK-----GRGEPYESSVFEAGGYKWRLVLYVNGNQNDGGNNHI 62

Query: 90  SLYL-VKAEDDIPTS-EVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIKTEWGFEKLISL 149
           SLY+ ++  + +P   EVNV     VY+    KYL V+D  V+R++  K EWG+ KLI L
Sbjct: 63  SLYVRIEETESLPKGWEVNVELKLFVYNGKQRKYLIVKDGIVKRYNDAKKEWGYGKLIPL 122

Query: 150 ETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFSQLDKNMY 209
            TF D++ G+L  D  +FG +I         E V+FI  P NN +TWK  +FS L+   Y
Sbjct: 123 TTFLDTNEGYLEQDIASFGAEIFSGTAVQVQEKVTFISNPPNNVFTWKILHFSNLEDKFY 182

Query: 210 ESDPFTVEGYRWKIRLHPKGCSQAR----------------------FGFLSLKL---MF 269
            SD F VE   W++  +PKG    R                      +G ++L+L     
Sbjct: 183 YSDDFLVEDRYWRLGFNPKGTGDGRSQAIPIFLYAQGHKPNAVATNTWGAVNLRLKNQRS 242

Query: 270 DSFKELPQGKYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVSTLKL 301
            +  ++    ++    D G+G    +SL + N+ +KGY  +D++I E ++  VS   +
Sbjct: 243 SNHAQIYSAAWYPTRSDYGVGVNTIISLAEFNDASKGYSVNDSIIFEAEMVKVSVTNI 295