MS027551 (gene) Bitter gourd (TR) v1

Overview


Name	MS027551
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Poly(A)-specific ribonuclease
Location	scaffold313: 1508144 .. 1509352 (-)
RNA-Seq Expression	MS027551
Synteny	MS027551

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAAAATGGACGATAAGCGTAATGACAATATATTTAGAGTGATGAAGTCACTGGAAAACATGGAGAAGGCGATTGTTAGAAAGTCAACAGCAGCTCCAAGATCCATCTGCCATAATCGAGAGGTTTGGTCTCACAATCTTCGTGAAGAATTAGAGCTACTGAATGAGTATCTTCCAGAGTTTCCAGTAGTCGCCATCGATACGGAGTTTCCAGGTATTGATCGGTTGCTTAGATGGATGATTGAAACTGTTTTTTAAGTTTAGCTTAAAAAAGGTTTTTACACTCGTGAACTCAATTGATGAAACAGGATTTCTCTTGTGCACTCCGAAAGGCATCTCGGAGGAAGAATTCTACCATTGTCTCCGATTCAATGTGAACCGATTGAACATACTCCAACTCGGTCTCACACTCGCCGATCAGAAGGGAAATATAGCAATGACTTGGGAGTTTAACTTCAGAGATTTTGATCACGTTAGCGATATGGAAGACGTTTCTTCGCTGGATTCATTTCTGAAGAACAAAGGGTTTGATTTCTACAGATTGAAGAAATACGGAATAGCTCAAGAGGATTTCACTACATTGTTTCTACCGATATGTCGTAGTGGTCAAATCAAGAGATGGATCACATTTCACGGCTTCTACGACATTGCTTATCTTCTCAAGCTGCTCAAAATCAAATCCATACCGATTTCCATGGCAACGTTTGCCACCACCGCTCAACATCTATTAGGTACTAATAAATCATCAATCAATTCAAAAACTTTAATTTGATGTATTACGGTTAATTGAATGTTTTATATACATACTTGATAGGTACGGTTAGCGATCTGAAGCATATGGCTCGCTACTGTGATGGACTGCTCGACGGCGACCTAGGGCTAAAAAAATTGGCCAAGCTGCTCGACGTGAAGCGTATAGGCACTGCACATTTTGCTGGTTCCGATAGTTTACTCACAGCTTCAGTATACACCAAGATGAAGCAAATTTTCAAGCTTCCGCTAGAGCAATGCGAAGGGTTTCTCTATGGCTTGCCTTATCCGATTAAGGGTTTTCGGTCAGTCGTGAAGATTATCGATCCATCCACCATGACTCCTCCTCCACCACTACCATCGAAGTTCTCAATATTGTCTTTTTGCAGCTCTACGCCGCTTTTGGTTGGTATTAAGTCTAGCAAGTTAAATGGTCATTCAGTCATAGTTGGTTGA

mRNA sequence

ATGGGGAAAATGGACGATAAGCGTAATGACAATATATTTAGAGTGATGAAGTCACTGGAAAACATGGAGAAGGCGATTGTTAGAAAGTCAACAGCAGCTCCAAGATCCATCTGCCATAATCGAGAGGTTTGGTCTCACAATCTTCGTGAAGAATTAGAGCTACTGAATGAGTATCTTCCAGAGTTTCCAGTAGTCGCCATCGATACGGAGTTTCCAGGATTTCTCTTGTGCACTCCGAAAGGCATCTCGGAGGAAGAATTCTACCATTGTCTCCGATTCAATGTGAACCGATTGAACATACTCCAACTCGGTCTCACACTCGCCGATCAGAAGGGAAATATAGCAATGACTTGGGAGTTTAACTTCAGAGATTTTGATCACGTTAGCGATATGGAAGACGTTTCTTCGCTGGATTCATTTCTGAAGAACAAAGGGTTTGATTTCTACAGATTGAAGAAATACGGAATAGCTCAAGAGGATTTCACTACATTGTTTCTACCGATATGTCGTAGTGGTCAAATCAAGAGATGGATCACATTTCACGGCTTCTACGACATTGCTTATCTTCTCAAGCTGCTCAAAATCAAATCCATACCGATTTCCATGGCAACGTTTGCCACCACCGCTCAACATCTATTAGGTACGGTTAGCGATCTGAAGCATATGGCTCGCTACTGTGATGGACTGCTCGACGGCGACCTAGGGCTAAAAAAATTGGCCAAGCTGCTCGACGTGAAGCGTATAGGCACTGCACATTTTGCTGGTTCCGATAGTTTACTCACAGCTTCAGTATACACCAAGATGAAGCAAATTTTCAAGCTTCCGCTAGAGCAATGCGAAGGGTTTCTCTATGGCTTGCCTTATCCGATTAAGGGTTTTCGGTCAGTCGTGAAGATTATCGATCCATCCACCATGACTCCTCCTCCACCACTACCATCGAAGTTCTCAATATTGTCTTTTTGCAGCTCTACGCCGCTTTTGGTTGGTATTAAGTCTAGCAAGTTAAATGGTCATTCAGTCATAGTTGGTTGA

Coding sequence (CDS)

ATGGGGAAAATGGACGATAAGCGTAATGACAATATATTTAGAGTGATGAAGTCACTGGAAAACATGGAGAAGGCGATTGTTAGAAAGTCAACAGCAGCTCCAAGATCCATCTGCCATAATCGAGAGGTTTGGTCTCACAATCTTCGTGAAGAATTAGAGCTACTGAATGAGTATCTTCCAGAGTTTCCAGTAGTCGCCATCGATACGGAGTTTCCAGGATTTCTCTTGTGCACTCCGAAAGGCATCTCGGAGGAAGAATTCTACCATTGTCTCCGATTCAATGTGAACCGATTGAACATACTCCAACTCGGTCTCACACTCGCCGATCAGAAGGGAAATATAGCAATGACTTGGGAGTTTAACTTCAGAGATTTTGATCACGTTAGCGATATGGAAGACGTTTCTTCGCTGGATTCATTTCTGAAGAACAAAGGGTTTGATTTCTACAGATTGAAGAAATACGGAATAGCTCAAGAGGATTTCACTACATTGTTTCTACCGATATGTCGTAGTGGTCAAATCAAGAGATGGATCACATTTCACGGCTTCTACGACATTGCTTATCTTCTCAAGCTGCTCAAAATCAAATCCATACCGATTTCCATGGCAACGTTTGCCACCACCGCTCAACATCTATTAGGTACGGTTAGCGATCTGAAGCATATGGCTCGCTACTGTGATGGACTGCTCGACGGCGACCTAGGGCTAAAAAAATTGGCCAAGCTGCTCGACGTGAAGCGTATAGGCACTGCACATTTTGCTGGTTCCGATAGTTTACTCACAGCTTCAGTATACACCAAGATGAAGCAAATTTTCAAGCTTCCGCTAGAGCAATGCGAAGGGTTTCTCTATGGCTTGCCTTATCCGATTAAGGGTTTTCGGTCAGTCGTGAAGATTATCGATCCATCCACCATGACTCCTCCTCCACCACTACCATCGAAGTTCTCAATATTGTCTTTTTGCAGCTCTACGCCGCTTTTGGTTGGTATTAAGTCTAGCAAGTTAAATGGTCATTCAGTCATAGTTGGTTGA

Protein sequence

MGKMDDKRNDNIFRVMKSLENMEKAIVRKSTAAPRSICHNREVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCEGFLYGLPYPIKGFRSVVKIIDPSTMTPPPPLPSKFSILSFCSSTPLLVGIKSSKLNGHSVIVG

Homology

[Close]

BLAST of MS027551 vs. NCBI nr
Match: XP_022146384.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 655.6 bits (1690), Expect = 2.4e-184
Identity = 323/340 (95.00%), Postives = 327/340 (96.18%), Query Frame = 0


Query: 4   MDDKRNDNIFRVMKSLENMEKAIVRKSTAAPRSICHNREVWSHNLREELELLNEYLPEFP 63
           MDDKRNDNIFRVMKSLENMEKAIVRKSTAAPRSICHNREVWS NLREELELLNEYLPEFP
Sbjct: 1   MDDKRNDNIFRVMKSLENMEKAIVRKSTAAPRSICHNREVWSLNLREELELLNEYLPEFP 60

Query: 64  VVAIDTEFPGFLLCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFR 123
           VV ID EFPGFL CTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFR
Sbjct: 61  VVVIDMEFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFR 120

Query: 124 DFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGF 183
           DFDHVSDMEDVSSLDSFLKNKGFDFY+LKKYGIAQEDFTTLFLPICRSGQIKRWITFHGF
Sbjct: 121 DFDHVSDMEDVSSLDSFLKNKGFDFYKLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGF 180

Query: 184 YDIAYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLL 243
           YDI YLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDG+LGLKKLAKLL
Sbjct: 181 YDITYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDGNLGLKKLAKLL 240

Query: 244 DVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCEGFLYGLPYPIKGFRSVVKIIDPS 303
           DVKRI  AHFA SD+L T SVYTKMKQIFKLPLEQCEGFLYGLPYPI GF+SV KIIDPS
Sbjct: 241 DVKRIDNAHFACSDNLFTTSVYTKMKQIFKLPLEQCEGFLYGLPYPINGFQSVAKIIDPS 300

Query: 304 TMTPPPPLPSKFSILSFCSSTPLLVGIKSSKLNGHSVIVG 344
           TMTPPPPLPSKFSILSFCSSTPLLVGIKSSKLNG SVIVG
Sbjct: 301 TMTPPPPLPSKFSILSFCSSTPLLVGIKSSKLNGPSVIVG 340

[Close]

BLAST of MS027551 vs. NCBI nr
Match: XP_022154798.1 (probable CCR4-associated factor 1 homolog 6 [Momordica charantia])

HSP 1 Score: 504.2 bits (1297), Expect = 8.8e-139
Identity = 256/350 (73.14%), Postives = 280/350 (80.00%), Query Frame = 0


Query: 22  MEKAIVRKSTAAPRSICHNREVWSHNLREELELLNEYLPEFPVVAIDTEF---------- 81
           MEK    KS  APRS+  NREVWS+NL +ELE+LNEYLPEFP+VAIDTEF          
Sbjct: 1   MEKTF-GKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNR 60

Query: 82  -------------------PGFLLCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKG 141
                              PGFL  TPKG+SEEEFY CLRFNVNRLNILQLGLTLA+Q G
Sbjct: 61  IYFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNG 120

Query: 142 NIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQEDFTTLFLPICRSG 201
           N+AMTWEFNFRDFDH+SDME VSSLDSFLKNKGFDFY+LK+ GIA EDFTTLFLPICRSG
Sbjct: 121 NVAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSG 180

Query: 202 QIKRWITFHGFYDIAYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDG 261
           +I+RWITFHGFYDIAYLLKLLKIK IPISMATFA TAQHLLGTVSDLKHM RYCDGLLDG
Sbjct: 181 RIERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDG 240

Query: 262 DLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCEGFLYGLPYPIKG 321
           DLGLKKLAKLLDVKRIG AHFAGSDSLLTA+VYTKMKQ+FK+P+EQ EGFLYGLP+ I+ 
Sbjct: 241 DLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQMFKVPVEQREGFLYGLPHRIQA 300

Query: 322 FRSVVKIIDPSTMTPPPPLPSKFSILSFCSSTPLLVGIKSSKLNGHSVIV 343
           F+ +VKI+DPS MTPPPP   +   L F S  P  + I SSKL GHSV V
Sbjct: 301 FQPIVKIVDPSAMTPPPP---RLPTLCFGSFPPFFIDIHSSKLKGHSVTV 346

[Close]

BLAST of MS027551 vs. NCBI nr
Match: XP_022153955.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 394.0 bits (1011), Expect = 1.3e-105
Identity = 204/288 (70.83%), Postives = 227/288 (78.82%), Query Frame = 0


Query: 22  MEKAIVRKSTAAPRSICHNREVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKG 81
           MEK I R S  APRS+   +EVWSHNL EELELLNEY+PEF VV ID             
Sbjct: 1   MEKTI-RNSMPAPRSVRKKQEVWSHNLHEELELLNEYIPEFLVVTID------------- 60

Query: 82  ISEEEFYHCLRFN-VNRLNILQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSF 141
           I        LR + + RLNILQLGLTL DQK N+AMT EFNFRDFDH+SDM+ +S LD F
Sbjct: 61  IGWYFGRKILRLSPIQRLNILQLGLTLVDQKENVAMTLEFNFRDFDHISDMKGISLLDLF 120

Query: 142 LKNKGFDFYRLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPI 201
           LKN GFDFY+LKK GI  E+FTTLFLPIC SG+I+R ITFHGFY+IAYLLK LKIK IPI
Sbjct: 121 LKNNGFDFYKLKKDGIVPENFTTLFLPICHSGRIERLITFHGFYEIAYLLKRLKIKFIPI 180

Query: 202 SMATFATTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLL 261
            MATFA TAQHLLG +S+LKHMARYCDGLLDG+L LKK AKLLDVKRIGTAHFAG DSLL
Sbjct: 181 FMATFAATAQHLLGAISNLKHMARYCDGLLDGNLALKKFAKLLDVKRIGTAHFAGFDSLL 240

Query: 262 TASVYTKMKQIFKLPLEQCEGFLYGLPYPIKGFRSVVKIIDPSTMTPP 309
           TAS+YTKMKQ+FKLPLEQCEGFLY LP+ IK F S++KII+PS  TPP
Sbjct: 241 TASIYTKMKQMFKLPLEQCEGFLYSLPHRIKAFWSILKIINPSATTPP 274

[Close]

BLAST of MS027551 vs. NCBI nr
Match: XP_022928830.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 385.2 bits (988), Expect = 6.0e-103
Identity = 183/266 (68.80%), Postives = 217/266 (81.58%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKGISEEEFYHCLRFNVNRLNI 100
           REVWSHNL EE+EL+  YL EFP+VAIDTEFPGFL  TPKG+S+EEFY CL+FNVNRL+I
Sbjct: 17  REVWSHNLLEEIELMKHYLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYQCLQFNVNRLSI 76

Query: 101 LQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQED 160
           LQLGLTL D  G I MTWEFNF+DFD +S+ME V SLDSFLKNKGF+F +L+  GIA ++
Sbjct: 77  LQLGLTLTDDNGKIGMTWEFNFQDFDQISNMEGVYSLDSFLKNKGFNFNKLRSCGIAPQE 136

Query: 161 FTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFATTAQHLLGTVSDLK 220
           F+  F P+ RSG+I++WITFHG YDIAYL+K   I S+P SM+ FA  A HLLGTV+DLK
Sbjct: 137 FSRQFKPVIRSGRIQKWITFHGLYDIAYLMKFNNIISMPTSMSMFAMIAAHLLGTVTDLK 196

Query: 221 HMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCE 280
           HMARY D L +G+LGLKKLAKLL + R+GTAHFAGSDSLLTASVY KMK I K+  +  E
Sbjct: 197 HMARYSDYLFNGNLGLKKLAKLLQINRVGTAHFAGSDSLLTASVYAKMKVIMKVEPKIHE 256

Query: 281 GFLYGLPYPIKGFRSVVKIIDPSTMT 307
           GFLYGLPYPI+G+R  V I+DPS +T
Sbjct: 257 GFLYGLPYPIQGYRPPVVIVDPSKLT 282

[Close]

BLAST of MS027551 vs. NCBI nr
Match: XP_023550519.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 381.7 bits (979), Expect = 6.6e-102
Identity = 183/266 (68.80%), Postives = 217/266 (81.58%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKGISEEEFYHCLRFNVNRLNI 100
           REVWS+NL EE+EL+  YL EFP+VA+DTEFPGFL  TPKG+S+EEFY CL+FNVNRL+I
Sbjct: 15  REVWSYNLLEEIELMKHYLSEFPIVAMDTEFPGFLRWTPKGVSDEEFYQCLQFNVNRLSI 74

Query: 101 LQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQED 160
           LQLGLTL D+ G I MTWEFNF+DFD +S+ME V SLDSFLKNKGF+F +L+  GIA E+
Sbjct: 75  LQLGLTLIDENGKIGMTWEFNFKDFDKISNMEGVYSLDSFLKNKGFNFNKLRSCGIAPEE 134

Query: 161 FTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFATTAQHLLGTVSDLK 220
           F+  F P+ RS +I+RWITFHG YDIAYL+K   I S+P SM+ FA  A HLLGTV+DLK
Sbjct: 135 FSRQFNPVIRSRRIQRWITFHGLYDIAYLMKFNNIISMPTSMSMFAIIAAHLLGTVTDLK 194

Query: 221 HMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCE 280
           HMARY D L +G+LGLKKLAKLL + RIGTAHFAGSDSLLTASVY KMK I K+  +  E
Sbjct: 195 HMARYSDYLFNGNLGLKKLAKLLQINRIGTAHFAGSDSLLTASVYAKMKAIMKVEPKIHE 254

Query: 281 GFLYGLPYPIKGFRSVVKIIDPSTMT 307
           GFLYGLPYPI+G+R  V I+DPS +T
Sbjct: 255 GFLYGLPYPIQGYRRPVVIVDPSKLT 280

[Close]

BLAST of MS027551 vs. ExPASy Swiss-Prot
Match: Q9SKZ2 (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 188.0 bits (476), Expect = 1.8e-46
Identity = 106/262 (40.46%), Postives = 154/262 (58.78%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKG---ISEEEFYHCLRFNVNR 100
           REVW+ NL  E+ L+ E + +FP VA+DTEFPG ++C P G    + E  Y  L+ NVN 
Sbjct: 13  REVWNDNLESEMALIREVVDDFPFVAMDTEFPG-IVCRPVGTFKTNTEYHYETLKTNVNI 72

Query: 101 LNILQLGLTLADQKGNIA--------MTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFY 160
           L ++QLGLT +D+KGN+           W+FNFR+FD  SD+    S++  L+  G DF 
Sbjct: 73  LKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIE-LLRQSGIDFV 132

Query: 161 RLKKYGIAQEDFTTLFLPICRSGQI----KRWITFHGFYDIAYLLKLLKIKSIPISMATF 220
           +  ++GI  + F  L +    SG +      W+TFH  YD  YLLKLL  +++P +   F
Sbjct: 133 KNNEFGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPETQTGF 192

Query: 221 ATTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVY 280
                     V D+KH+ ++C+ L     GL KLA+LLDV+R+G  H AGSDSLLT+  +
Sbjct: 193 FEMISVYFPRVYDIKHLMKFCNSLHG---GLNKLAELLDVERVGICHQAGSDSLLTSCTF 252

Query: 281 TKMKQIFKL-PLEQCEGFLYGL 287
            K+++ F +  +E+  G LYGL
Sbjct: 253 RKLQENFFIGSMEKYSGVLYGL 266

[Close]

BLAST of MS027551 vs. ExPASy Swiss-Prot
Match: Q9S9P2 (Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=CAF1-2 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.4e-45
Identity = 105/267 (39.33%), Postives = 154/267 (57.68%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCT--------PKGISEEEFYHCLR 100
           REVW+HNL +E+ L+ + + +FP VA+DTEFPG +  T        P  I  E  Y  L+
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTLK 76

Query: 101 FNVNRLNILQLGLTLADQKGNI-------AMTWEFNFRDFDHVSDMEDVSSLDSFLKNKG 160
            NVN L ++QLGLTL+D+KGN+          W+FNFR+F+ +SDM  + S++  L+   
Sbjct: 77  ANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKSA 136

Query: 161 FDFYRLKKYGIAQEDFTTLFL--PICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMA 220
            D  +  + G+  + F  L +   +  + +I  W+TFH  YD  YLLKLL  K +P  ++
Sbjct: 137 IDLEKNNECGVDAKRFAELLMGSGVVLNDKI-HWVTFHCGYDFGYLLKLLSGKELPEEIS 196

Query: 221 TFATTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTAS 280
            F    +     V D+K++  +C  L     GL+K+A+LL VKR+G +H AGSDSLLT  
Sbjct: 197 DFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAELLGVKRVGISHQAGSDSLLTLR 256

Query: 281 VYTKMKQIFKL-PLEQCEGFLYGLPYP 290
            + KMK+ F    L +  GFL+GL  P
Sbjct: 257 TFIKMKEFFFTGSLLKYSGFLFGLDNP 278

[Close]

BLAST of MS027551 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.3e-44
Identity = 105/261 (40.23%), Postives = 155/261 (59.39%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKGI---SEEEFYHCLRFNVNR 100
           REVW+ NL+EE++L+ + + +FP VA+DTEFPG ++  P G    + +  Y  L+ NVN 
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPG-IVVRPVGTFKSNADYHYETLKTNVNI 72

Query: 101 LNILQLGLTLADQKGNIA-------MTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYR 160
           L ++QLGLT ++++GN+          W+FNFR+FD  SD+  + S++  LK  G D  +
Sbjct: 73  LKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAK 132

Query: 161 LKKYGIAQEDFTTLFLPICRSGQI----KRWITFHGFYDIAYLLKLLKIKSIPISMATFA 220
               GI  + F  L +    SG +      W+TFH  YD  YLLKLL  +++P S   F 
Sbjct: 133 NTLDGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFF 192

Query: 221 TTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYT 280
                   TV D+KH+ ++C+ L     GL KLA+LL+V+R+G  H AGSDSLLT+  + 
Sbjct: 193 KLINVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTFR 252

Query: 281 KMKQIFKL-PLEQCEGFLYGL 287
           K+K+ F + PL +  G LYGL
Sbjct: 253 KLKENFFVGPLHKYSGVLYGL 265

[Close]

BLAST of MS027551 vs. ExPASy Swiss-Prot
Match: Q9SHJ0 (Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=CAF1-1 PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.7e-42
Identity = 100/274 (36.50%), Postives = 149/274 (54.38%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKGISEEEFYHCLRFNVNRLNI 100
           R VW  N+ EE+  + E L  FP++A DTE+PG +  T    S +E Y  ++ NV    +
Sbjct: 10  RRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVENTKL 69

Query: 101 LQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQ-- 160
           +Q G TL + KG I   WE NF +F   SD  +  S++ FL+  G D  +++  G+    
Sbjct: 70  IQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIE-FLRRHGLDLQKIRDEGVDMFG 129

Query: 161 EDFTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFATTAQHLLGTVSD 220
             F    + + RS +   ++TF G YD AY L +L    +P +   FAT    + G V D
Sbjct: 130 YGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILNHGKLPETHGEFATEVVKVFGQVYD 189

Query: 221 LKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQ 280
            K MA +C+GL +  LGL KLA+LL + R+G AH AGSDSL+TA V+ K+K +++     
Sbjct: 190 TKVMAGFCEGLGE-HLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE-DSRF 249

Query: 281 CEGFLYGLPYPIKGFRSVVKIIDPSTMTPPPPLP 313
             G +YG+     G  ++V    P+ + P P LP
Sbjct: 250 ARGLIYGI-----GKSNLVAAPAPAPV-PEPTLP 274

[Close]

BLAST of MS027551 vs. ExPASy Swiss-Prot
Match: Q9LXM2 (Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=CAF1-9 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 6.7e-41
Identity = 96/263 (36.50%), Postives = 152/263 (57.79%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTP-KGISEEEFYHCLRFNVNRLN 100
           REVW+ NL  E EL++E + ++P +++DTEFPG +  +  +  + ++ Y  L+ NV+ L+
Sbjct: 20  REVWAENLESEFELISEIIDDYPFISMDTEFPGVIFKSDLRFTNPDDLYTLLKANVDALS 79

Query: 101 ILQLGLTLADQKGNI---------AMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYR 160
           ++Q+GLTL+D  GN+            WEFNFRDFD   D     S++  L+ +G DF R
Sbjct: 80  LIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAPDSIE-LLRRQGIDFER 139

Query: 161 LKKYGIAQEDFTTLFLP---ICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFAT 220
             + G+  E F  L +    +C   +   W+TFH  YD  YL+K+L  + +P ++  F  
Sbjct: 140 NCRDGVESERFAELMMSSGLVC--NEEVSWVTFHSAYDFGYLMKILTRRELPGALGEFKR 199

Query: 221 TAQHLLG-TVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKR-IGTAHFAGSDSLLTASVY 280
             + L G  V D+KHM ++C+  L G  GL ++A+ L+V R +G  H AGSDSLLT   +
Sbjct: 200 VMRVLFGERVYDVKHMMKFCERRLFG--GLDRVARTLEVNRAVGKCHQAGSDSLLTWHAF 259

Query: 281 TKMKQIFKLP--LEQCEGFLYGL 287
            +M+ ++ +    E+  G LYGL
Sbjct: 260 QRMRDLYFVQDGPEKHAGVLYGL 277

[Close]

BLAST of MS027551 vs. ExPASy TrEMBL
Match: A0A6J1CXZ5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=3 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.1e-184
Identity = 323/340 (95.00%), Postives = 327/340 (96.18%), Query Frame = 0


Query: 4   MDDKRNDNIFRVMKSLENMEKAIVRKSTAAPRSICHNREVWSHNLREELELLNEYLPEFP 63
           MDDKRNDNIFRVMKSLENMEKAIVRKSTAAPRSICHNREVWS NLREELELLNEYLPEFP
Sbjct: 1   MDDKRNDNIFRVMKSLENMEKAIVRKSTAAPRSICHNREVWSLNLREELELLNEYLPEFP 60

Query: 64  VVAIDTEFPGFLLCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFR 123
           VV ID EFPGFL CTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFR
Sbjct: 61  VVVIDMEFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFR 120

Query: 124 DFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGF 183
           DFDHVSDMEDVSSLDSFLKNKGFDFY+LKKYGIAQEDFTTLFLPICRSGQIKRWITFHGF
Sbjct: 121 DFDHVSDMEDVSSLDSFLKNKGFDFYKLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGF 180

Query: 184 YDIAYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLL 243
           YDI YLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDG+LGLKKLAKLL
Sbjct: 181 YDITYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDGNLGLKKLAKLL 240

Query: 244 DVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCEGFLYGLPYPIKGFRSVVKIIDPS 303
           DVKRI  AHFA SD+L T SVYTKMKQIFKLPLEQCEGFLYGLPYPI GF+SV KIIDPS
Sbjct: 241 DVKRIDNAHFACSDNLFTTSVYTKMKQIFKLPLEQCEGFLYGLPYPINGFQSVAKIIDPS 300

Query: 304 TMTPPPPLPSKFSILSFCSSTPLLVGIKSSKLNGHSVIVG 344
           TMTPPPPLPSKFSILSFCSSTPLLVGIKSSKLNG SVIVG
Sbjct: 301 TMTPPPPLPSKFSILSFCSSTPLLVGIKSSKLNGPSVIVG 340

[Close]

BLAST of MS027551 vs. ExPASy TrEMBL
Match: A0A6J1DLA5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 4.3e-139
Identity = 256/350 (73.14%), Postives = 280/350 (80.00%), Query Frame = 0


Query: 22  MEKAIVRKSTAAPRSICHNREVWSHNLREELELLNEYLPEFPVVAIDTEF---------- 81
           MEK    KS  APRS+  NREVWS+NL +ELE+LNEYLPEFP+VAIDTEF          
Sbjct: 1   MEKTF-GKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNR 60

Query: 82  -------------------PGFLLCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKG 141
                              PGFL  TPKG+SEEEFY CLRFNVNRLNILQLGLTLA+Q G
Sbjct: 61  IYFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNG 120

Query: 142 NIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQEDFTTLFLPICRSG 201
           N+AMTWEFNFRDFDH+SDME VSSLDSFLKNKGFDFY+LK+ GIA EDFTTLFLPICRSG
Sbjct: 121 NVAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSG 180

Query: 202 QIKRWITFHGFYDIAYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDG 261
           +I+RWITFHGFYDIAYLLKLLKIK IPISMATFA TAQHLLGTVSDLKHM RYCDGLLDG
Sbjct: 181 RIERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDG 240

Query: 262 DLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCEGFLYGLPYPIKG 321
           DLGLKKLAKLLDVKRIG AHFAGSDSLLTA+VYTKMKQ+FK+P+EQ EGFLYGLP+ I+ 
Sbjct: 241 DLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQMFKVPVEQREGFLYGLPHRIQA 300

Query: 322 FRSVVKIIDPSTMTPPPPLPSKFSILSFCSSTPLLVGIKSSKLNGHSVIV 343
           F+ +VKI+DPS MTPPPP   +   L F S  P  + I SSKL GHSV V
Sbjct: 301 FQPIVKIVDPSAMTPPPP---RLPTLCFGSFPPFFIDIHSSKLKGHSVTV 346

[Close]

BLAST of MS027551 vs. ExPASy TrEMBL
Match: A0A6J1DIB1 (putative CCR4-associated factor 1 homolog 8 OS=Momordica charantia OX=3673 GN=LOC111021342 PE=4 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 6.2e-106
Identity = 204/288 (70.83%), Postives = 227/288 (78.82%), Query Frame = 0


Query: 22  MEKAIVRKSTAAPRSICHNREVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKG 81
           MEK I R S  APRS+   +EVWSHNL EELELLNEY+PEF VV ID             
Sbjct: 1   MEKTI-RNSMPAPRSVRKKQEVWSHNLHEELELLNEYIPEFLVVTID------------- 60

Query: 82  ISEEEFYHCLRFN-VNRLNILQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSF 141
           I        LR + + RLNILQLGLTL DQK N+AMT EFNFRDFDH+SDM+ +S LD F
Sbjct: 61  IGWYFGRKILRLSPIQRLNILQLGLTLVDQKENVAMTLEFNFRDFDHISDMKGISLLDLF 120

Query: 142 LKNKGFDFYRLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPI 201
           LKN GFDFY+LKK GI  E+FTTLFLPIC SG+I+R ITFHGFY+IAYLLK LKIK IPI
Sbjct: 121 LKNNGFDFYKLKKDGIVPENFTTLFLPICHSGRIERLITFHGFYEIAYLLKRLKIKFIPI 180

Query: 202 SMATFATTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLL 261
            MATFA TAQHLLG +S+LKHMARYCDGLLDG+L LKK AKLLDVKRIGTAHFAG DSLL
Sbjct: 181 FMATFAATAQHLLGAISNLKHMARYCDGLLDGNLALKKFAKLLDVKRIGTAHFAGFDSLL 240

Query: 262 TASVYTKMKQIFKLPLEQCEGFLYGLPYPIKGFRSVVKIIDPSTMTPP 309
           TAS+YTKMKQ+FKLPLEQCEGFLY LP+ IK F S++KII+PS  TPP
Sbjct: 241 TASIYTKMKQMFKLPLEQCEGFLYSLPHRIKAFWSILKIINPSATTPP 274

[Close]

BLAST of MS027551 vs. ExPASy TrEMBL
Match: A0A6J1ESM7 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 2.9e-103
Identity = 183/266 (68.80%), Postives = 217/266 (81.58%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKGISEEEFYHCLRFNVNRLNI 100
           REVWSHNL EE+EL+  YL EFP+VAIDTEFPGFL  TPKG+S+EEFY CL+FNVNRL+I
Sbjct: 17  REVWSHNLLEEIELMKHYLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYQCLQFNVNRLSI 76

Query: 101 LQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQED 160
           LQLGLTL D  G I MTWEFNF+DFD +S+ME V SLDSFLKNKGF+F +L+  GIA ++
Sbjct: 77  LQLGLTLTDDNGKIGMTWEFNFQDFDQISNMEGVYSLDSFLKNKGFNFNKLRSCGIAPQE 136

Query: 161 FTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFATTAQHLLGTVSDLK 220
           F+  F P+ RSG+I++WITFHG YDIAYL+K   I S+P SM+ FA  A HLLGTV+DLK
Sbjct: 137 FSRQFKPVIRSGRIQKWITFHGLYDIAYLMKFNNIISMPTSMSMFAMIAAHLLGTVTDLK 196

Query: 221 HMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCE 280
           HMARY D L +G+LGLKKLAKLL + R+GTAHFAGSDSLLTASVY KMK I K+  +  E
Sbjct: 197 HMARYSDYLFNGNLGLKKLAKLLQINRVGTAHFAGSDSLLTASVYAKMKVIMKVEPKIHE 256

Query: 281 GFLYGLPYPIKGFRSVVKIIDPSTMT 307
           GFLYGLPYPI+G+R  V I+DPS +T
Sbjct: 257 GFLYGLPYPIQGYRPPVVIVDPSKLT 282

[Close]

BLAST of MS027551 vs. ExPASy TrEMBL
Match: A0A6J1HL76 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463977 PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 7.1e-102
Identity = 186/266 (69.92%), Postives = 214/266 (80.45%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKGISEEEFYHCLRFNVNRLNI 100
           R+VWSHNL EE+EL+ + L EFP+VAIDTEFPGFL  TPKG+S+EEFY CL+FNVNRL+I
Sbjct: 17  RDVWSHNLLEEIELIKQCLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYECLQFNVNRLSI 76

Query: 101 LQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQED 160
           LQLGLTL +  G I MTWEFNFRDFD +S+ME V SLDSFLKNKGF+F  L+ YGIA E+
Sbjct: 77  LQLGLTLTNDNGKIGMTWEFNFRDFDKISNMEGVYSLDSFLKNKGFNFNTLRSYGIAPEE 136

Query: 161 FTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFATTAQHLLGTVSDLK 220
           F T F PI RSG+I RWITFHG YDIAYL+K   I+ +P SM+ FA TA H+LGTV+DLK
Sbjct: 137 FGTQFSPILRSGRIHRWITFHGLYDIAYLMKFTNIRCMPTSMSIFAITAAHILGTVTDLK 196

Query: 221 HMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCE 280
           HMA Y   LLDG+LGLKKLAKLL V R GTAHFAGSDSLLTASVY KMK I K+     E
Sbjct: 197 HMAWY--SLLDGNLGLKKLAKLLQVNRTGTAHFAGSDSLLTASVYAKMKIIMKVEPNIHE 256

Query: 281 GFLYGLPYPIKGFRSVVKIIDPSTMT 307
           GFLYGLPYPI+G+R  V I+DPS +T
Sbjct: 257 GFLYGLPYPIQGYRRPVVIVDPSKLT 280

[Close]

BLAST of MS027551 vs. TAIR 10
Match: AT2G32070.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 188.0 bits (476), Expect = 1.3e-47
Identity = 106/262 (40.46%), Postives = 154/262 (58.78%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKG---ISEEEFYHCLRFNVNR 100
           REVW+ NL  E+ L+ E + +FP VA+DTEFPG ++C P G    + E  Y  L+ NVN 
Sbjct: 13  REVWNDNLESEMALIREVVDDFPFVAMDTEFPG-IVCRPVGTFKTNTEYHYETLKTNVNI 72

Query: 101 LNILQLGLTLADQKGNIA--------MTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFY 160
           L ++QLGLT +D+KGN+           W+FNFR+FD  SD+    S++  L+  G DF 
Sbjct: 73  LKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIE-LLRQSGIDFV 132

Query: 161 RLKKYGIAQEDFTTLFLPICRSGQI----KRWITFHGFYDIAYLLKLLKIKSIPISMATF 220
           +  ++GI  + F  L +    SG +      W+TFH  YD  YLLKLL  +++P +   F
Sbjct: 133 KNNEFGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPETQTGF 192

Query: 221 ATTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVY 280
                     V D+KH+ ++C+ L     GL KLA+LLDV+R+G  H AGSDSLLT+  +
Sbjct: 193 FEMISVYFPRVYDIKHLMKFCNSLHG---GLNKLAELLDVERVGICHQAGSDSLLTSCTF 252

Query: 281 TKMKQIFKL-PLEQCEGFLYGL 287
            K+++ F +  +E+  G LYGL
Sbjct: 253 RKLQENFFIGSMEKYSGVLYGL 266

[Close]

BLAST of MS027551 vs. TAIR 10
Match: AT1G15920.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 183.7 bits (465), Expect = 2.4e-46
Identity = 105/267 (39.33%), Postives = 154/267 (57.68%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCT--------PKGISEEEFYHCLR 100
           REVW+HNL +E+ L+ + + +FP VA+DTEFPG +  T        P  I  E  Y  L+
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTLK 76

Query: 101 FNVNRLNILQLGLTLADQKGNI-------AMTWEFNFRDFDHVSDMEDVSSLDSFLKNKG 160
            NVN L ++QLGLTL+D+KGN+          W+FNFR+F+ +SDM  + S++  L+   
Sbjct: 77  ANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKSA 136

Query: 161 FDFYRLKKYGIAQEDFTTLFL--PICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMA 220
            D  +  + G+  + F  L +   +  + +I  W+TFH  YD  YLLKLL  K +P  ++
Sbjct: 137 IDLEKNNECGVDAKRFAELLMGSGVVLNDKI-HWVTFHCGYDFGYLLKLLSGKELPEEIS 196

Query: 221 TFATTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTAS 280
            F    +     V D+K++  +C  L     GL+K+A+LL VKR+G +H AGSDSLLT  
Sbjct: 197 DFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAELLGVKRVGISHQAGSDSLLTLR 256

Query: 281 VYTKMKQIFKL-PLEQCEGFLYGLPYP 290
            + KMK+ F    L +  GFL+GL  P
Sbjct: 257 TFIKMKEFFFTGSLLKYSGFLFGLDNP 278

[Close]

BLAST of MS027551 vs. TAIR 10
Match: AT1G15920.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 183.7 bits (465), Expect = 2.4e-46
Identity = 105/267 (39.33%), Postives = 154/267 (57.68%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCT--------PKGISEEEFYHCLR 100
           REVW+HNL +E+ L+ + + +FP VA+DTEFPG +  T        P  I  E  Y  L+
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTLK 76

Query: 101 FNVNRLNILQLGLTLADQKGNI-------AMTWEFNFRDFDHVSDMEDVSSLDSFLKNKG 160
            NVN L ++QLGLTL+D+KGN+          W+FNFR+F+ +SDM  + S++  L+   
Sbjct: 77  ANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKSA 136

Query: 161 FDFYRLKKYGIAQEDFTTLFL--PICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMA 220
            D  +  + G+  + F  L +   +  + +I  W+TFH  YD  YLLKLL  K +P  ++
Sbjct: 137 IDLEKNNECGVDAKRFAELLMGSGVVLNDKI-HWVTFHCGYDFGYLLKLLSGKELPEEIS 196

Query: 221 TFATTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTAS 280
            F    +     V D+K++  +C  L     GL+K+A+LL VKR+G +H AGSDSLLT  
Sbjct: 197 DFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAELLGVKRVGISHQAGSDSLLTLR 256

Query: 281 VYTKMKQIFKL-PLEQCEGFLYGLPYP 290
            + KMK+ F    L +  GFL+GL  P
Sbjct: 257 TFIKMKEFFFTGSLLKYSGFLFGLDNP 278

[Close]

BLAST of MS027551 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 181.8 bits (460), Expect = 9.3e-46
Identity = 105/261 (40.23%), Postives = 155/261 (59.39%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKGI---SEEEFYHCLRFNVNR 100
           REVW+ NL+EE++L+ + + +FP VA+DTEFPG ++  P G    + +  Y  L+ NVN 
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPG-IVVRPVGTFKSNADYHYETLKTNVNI 72

Query: 101 LNILQLGLTLADQKGNIA-------MTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYR 160
           L ++QLGLT ++++GN+          W+FNFR+FD  SD+  + S++  LK  G D  +
Sbjct: 73  LKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAK 132

Query: 161 LKKYGIAQEDFTTLFLPICRSGQI----KRWITFHGFYDIAYLLKLLKIKSIPISMATFA 220
               GI  + F  L +    SG +      W+TFH  YD  YLLKLL  +++P S   F 
Sbjct: 133 NTLDGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFF 192

Query: 221 TTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYT 280
                   TV D+KH+ ++C+ L     GL KLA+LL+V+R+G  H AGSDSLLT+  + 
Sbjct: 193 KLINVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTFR 252

Query: 281 KMKQIFKL-PLEQCEGFLYGL 287
           K+K+ F + PL +  G LYGL
Sbjct: 253 KLKENFFVGPLHKYSGVLYGL 265

[Close]

BLAST of MS027551 vs. TAIR 10
Match: AT1G80780.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 181.8 bits (460), Expect = 9.3e-46
Identity = 105/261 (40.23%), Postives = 155/261 (59.39%), Query Frame = 0


Query: 41  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLLCTPKGI---SEEEFYHCLRFNVNR 100
           REVW+ NL+EE++L+ + + +FP VA+DTEFPG ++  P G    + +  Y  L+ NVN 
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPG-IVVRPVGTFKSNADYHYETLKTNVNI 72

Query: 101 LNILQLGLTLADQKGNIA-------MTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYR 160
           L ++QLGLT ++++GN+          W+FNFR+FD  SD+  + S++  LK  G D  +
Sbjct: 73  LKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAK 132

Query: 161 LKKYGIAQEDFTTLFLPICRSGQI----KRWITFHGFYDIAYLLKLLKIKSIPISMATFA 220
               GI  + F  L +    SG +      W+TFH  YD  YLLKLL  +++P S   F 
Sbjct: 133 NTLDGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFF 192

Query: 221 TTAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYT 280
                   TV D+KH+ ++C+ L     GL KLA+LL+V+R+G  H AGSDSLLT+  + 
Sbjct: 193 KLINVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTFR 252

Query: 281 KMKQIFKL-PLEQCEGFLYGL 287
           K+K+ F + PL +  G LYGL
Sbjct: 253 KLKENFFVGPLHKYSGVLYGL 265

The following BLAST results are available for this feature:

BLAST of MS027551 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146384.1	2.4e-184	95.00	putative CCR4-associated factor 1 homolog 8 [Momordica charantia]	[more]
XP_022154798.1	8.8e-139	73.14	probable CCR4-associated factor 1 homolog 6 [Momordica charantia]	[more]
XP_022153955.1	1.3e-105	70.83	putative CCR4-associated factor 1 homolog 8 [Momordica charantia]	[more]
XP_022928830.1	6.0e-103	68.80	putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata]	[more]
XP_023550519.1	6.6e-102	68.80	putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS027551 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SKZ2	1.8e-46	40.46	Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9S9P2	3.4e-45	39.33	Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SAI2	1.3e-44	40.23	Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SHJ0	4.7e-42	36.50	Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9LXM2	6.7e-41	36.50	Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]

BLAST of MS027551 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXZ5	1.1e-184	95.00	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=...	[more]
A0A6J1DLA5	4.3e-139	73.14	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=...	[more]
A0A6J1DIB1	6.2e-106	70.83	putative CCR4-associated factor 1 homolog 8 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1ESM7	2.9e-103	68.80	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3...	[more]
A0A6J1HL76	7.1e-102	69.92	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463977 PE=3...	[more]

BLAST of MS027551 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32070.1	1.3e-47	40.46	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G15920.2	2.4e-46	39.33	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G15920.1	2.4e-46	39.33	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.1	9.3e-46	40.23	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.2	9.3e-46	40.23	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 26..288 e-value: 5.3E-72 score: 244.1
IPR006941	Ribonuclease CAF1	PFAM	PF04857	CAF1	coord: 43..156 e-value: 7.8E-8 score: 31.9
None	No IPR available	PANTHER	PTHR10797:SF57	CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATED	coord: 40..293
IPR039637	CCR4-NOT transcription complex subunit 7/8/Pop2	PANTHER	PTHR10797	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT	coord: 40..293
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 34..284

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS027551.1	MS027551.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0030014	CCR4-NOT complex
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004535	poly(A)-specific ribonuclease activity