MS027291 (gene) Bitter gourd (TR) v1

Overview


Name	MS027291
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Poly(A)-specific ribonuclease
Location	scaffold373: 666780 .. 667133 (+)
RNA-Seq Expression	MS027291
Synteny	MS027291

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCACATTTCATGGCTTCTACGACATCGCTTATCTTCTGAAGCTGCTCAAAATCAAATCCATACCAATTTCCATGGCAATGTTTGCAGCCACCGCTCAGCATCTATTAGGTACTAATGAATCGCCAATCAATTTAAAAGCTTAATTTGATGTGTTATGGTAAATTTAATGTTTTATATTTACTTGATAGGTACTGTCACTGATTTGAAGCATATGGCTCGCTATTGCGATGGATTGCTCGACAGCGACCTAGGGCTAAAAAAATTGGCAAAGCTACTCGACGTGAAACGAATAGGCATAGCACATTTTGCTGGTTCTGATAGTTTACTCACCGCTGCAGTATACACCTAG

mRNA sequence

ATGGATCACATTTCATGGCTTCTACGACATCGCTTATCTTCTGAAGCTGCTCAAAATCAAATCCATACCAATTTCCATGGCAATGTTTGCAGCCACCGCTCAGCATCTATTAGGTACTAATGAATCGCCAATCAATTTAAAAGCTTAATTTGATGTGTTATGGTAAATTTAATGTTTTATATTTACTTGATAGGTACTGTCACTGATTTGAAGCATATGGCTCGCTATTGCGATGGATTGCTCGACAGCGACCTAGGGCTAAAAAAATTGGCAAAGCTACTCGACGTGAAACGAATAGGCATAGCACATTTTGCTGGTTCTGATAGTTTACTCACCGCTGCAGTATACACCTAG

Coding sequence (CDS)

ATGGATCACATTTCATGGCTTCTACGACATCGCTTATCTTCTGAAGCTGCTCAAAATCAAATCCATACCAATTTCCATGGCAATGTTTGCAGCCACCGCTCAGCATCTATTAGGTACTAATGAATCGCCAATCAATTTAAAAGCTTAATTTGATGTGTTATGGTAAATTTAATGTTTTATATTTACTTGATAGGTACTGTCACTGATTTGAAGCATATGGCTCGCTATTGCGATGGATTGCTCGACAGCGACCTAGGGCTAAAAAAATTGGCAAAGCTACTCGACGTGAAACGAATAGGCATAGCACATTTTGCTGGTTCTGATAGTTTACTCACCGCTGCAGTATACACCTAG

Protein sequence

MDHISWLLRHRLSSEAAQNQIHTNFHGNVCSHRSASIRYUUIANQFKSLIUCVMVNLMFYIYLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT

Homology

[Close]

BLAST of MS027291 vs. NCBI nr
Match: XP_022158209.1 (probable CCR4-associated factor 1 homolog 5 [Momordica charantia])

HSP 1 Score: 110.5 bits (275), Expect = 9.7e-21
Identity = 54/56 (96.43%), Postives = 56/56 (100.00%), Query Frame = 0


Query: 62  YLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 118
           +L+GTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT
Sbjct: 81  HLLGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 136

[Close]

BLAST of MS027291 vs. NCBI nr
Match: XP_022154798.1 (probable CCR4-associated factor 1 homolog 6 [Momordica charantia])

HSP 1 Score: 105.9 bits (263), Expect = 2.4e-19
Identity = 51/56 (91.07%), Postives = 54/56 (96.43%), Query Frame = 0


Query: 62  YLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 118
           +L+GTV+DLKHM RYCDGLLD DLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT
Sbjct: 218 HLLGTVSDLKHMCRYCDGLLDGDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 273

[Close]

BLAST of MS027291 vs. NCBI nr
Match: XP_038888808.1 (probable CCR4-associated factor 1 homolog 6 [Benincasa hispida])

HSP 1 Score: 97.1 bits (240), Expect = 1.1e-16
Identity = 47/54 (87.04%), Postives = 50/54 (92.59%), Query Frame = 0


Query: 63  LIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVY 117
           L+GTVTDLKHMARYCDGLLD +LGLKKLAKLL V RIG AHFAGSDSLLTA+VY
Sbjct: 97  LLGTVTDLKHMARYCDGLLDDNLGLKKLAKLLGVNRIGTAHFAGSDSLLTASVY 150

[Close]

BLAST of MS027291 vs. NCBI nr
Match: XP_022153955.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 91.7 bits (226), Expect = 4.6e-15
Identity = 42/56 (75.00%), Postives = 50/56 (89.29%), Query Frame = 0


Query: 62  YLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 118
           +L+G +++LKHMARYCDGLLD +L LKK AKLLDVKRIG AHFAG DSLLTA++YT
Sbjct: 177 HLLGAISNLKHMARYCDGLLDGNLALKKFAKLLDVKRIGTAHFAGFDSLLTASIYT 232

[Close]

BLAST of MS027291 vs. NCBI nr
Match: XP_022146384.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 91.3 bits (225), Expect = 6.1e-15
Identity = 44/56 (78.57%), Postives = 50/56 (89.29%), Query Frame = 0


Query: 62  YLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 118
           +L+GTV+DLKHMARYCDGLLD +LGLKKLAKLLDVKRI  AHFA SD+L T +VYT
Sbjct: 208 HLLGTVSDLKHMARYCDGLLDGNLGLKKLAKLLDVKRIDNAHFACSDNLFTTSVYT 263

[Close]

BLAST of MS027291 vs. ExPASy Swiss-Prot
Match: Q9SHJ0 (Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=CAF1-1 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 9.7e-08
Identity = 30/56 (53.57%), Postives = 39/56 (69.64%), Query Frame = 0


Query: 61  IYLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVY 117
           + + G V D K MA +C+G L   LGL KLA+LL + R+G AH AGSDSL+TA V+
Sbjct: 180 VKVFGQVYDTKVMAGFCEG-LGEHLGLSKLAQLLQITRVGRAHHAGSDSLMTALVF 234

[Close]

BLAST of MS027291 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 2.8e-07
Identity = 27/51 (52.94%), Postives = 38/51 (74.51%), Query Frame = 0


Query: 66  TVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVY 117
           TV D+KH+ ++C+ L     GL KLA+LL+V+R+GI H AGSDSLLT+  +
Sbjct: 196 TVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTF 243

[Close]

BLAST of MS027291 vs. ExPASy Swiss-Prot
Match: Q9SKZ2 (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 4.8e-07
Identity = 27/50 (54.00%), Postives = 37/50 (74.00%), Query Frame = 0


Query: 67  VTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVY 117
           V D+KH+ ++C+ L     GL KLA+LLDV+R+GI H AGSDSLLT+  +
Sbjct: 198 VYDIKHLMKFCNSLHG---GLNKLAELLDVERVGICHQAGSDSLLTSCTF 244

[Close]

BLAST of MS027291 vs. ExPASy Swiss-Prot
Match: Q9LEU4 (Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=CAF1-10 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 4.8e-07
Identity = 28/55 (50.91%), Postives = 39/55 (70.91%), Query Frame = 0


Query: 58  MFYIYLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLT 113
           + Y+Y   TV D+KH+  +C+GL     GL +LA+L+ V+R+GI H AGSDSLLT
Sbjct: 189 LLYVY-FPTVYDIKHLMTFCNGLFG---GLNRLAELMGVERVGICHQAGSDSLLT 239

[Close]

BLAST of MS027291 vs. ExPASy Swiss-Prot
Match: Q9SFX6 (Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=CAF1-3 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 4.1e-06
Identity = 27/51 (52.94%), Postives = 36/51 (70.59%), Query Frame = 0


Query: 67  VTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 118
           V D+K +A  C G L S  GL+++A +L +KR+G AH AGSDS LTA V+T
Sbjct: 183 VFDVKKIAESCSG-LSSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFT 232

[Close]

BLAST of MS027291 vs. ExPASy TrEMBL
Match: A0A6J1DYQ7 (probable CCR4-associated factor 1 homolog 5 OS=Momordica charantia OX=3673 GN=LOC111024743 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 4.7e-21
Identity = 54/56 (96.43%), Postives = 56/56 (100.00%), Query Frame = 0


Query: 62  YLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 118
           +L+GTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT
Sbjct: 81  HLLGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 136

[Close]

BLAST of MS027291 vs. ExPASy TrEMBL
Match: A0A6J1DLA5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.2e-19
Identity = 51/56 (91.07%), Postives = 54/56 (96.43%), Query Frame = 0


Query: 62  YLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 118
           +L+GTV+DLKHM RYCDGLLD DLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT
Sbjct: 218 HLLGTVSDLKHMCRYCDGLLDGDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 273

[Close]

BLAST of MS027291 vs. ExPASy TrEMBL
Match: A0A6J1DIB1 (putative CCR4-associated factor 1 homolog 8 OS=Momordica charantia OX=3673 GN=LOC111021342 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 2.2e-15
Identity = 42/56 (75.00%), Postives = 50/56 (89.29%), Query Frame = 0


Query: 62  YLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 118
           +L+G +++LKHMARYCDGLLD +L LKK AKLLDVKRIG AHFAG DSLLTA++YT
Sbjct: 177 HLLGAISNLKHMARYCDGLLDGNLALKKFAKLLDVKRIGTAHFAGFDSLLTASIYT 232

[Close]

BLAST of MS027291 vs. ExPASy TrEMBL
Match: A0A6J1CXZ5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.9e-15
Identity = 44/56 (78.57%), Postives = 50/56 (89.29%), Query Frame = 0


Query: 62  YLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYT 118
           +L+GTV+DLKHMARYCDGLLD +LGLKKLAKLLDVKRI  AHFA SD+L T +VYT
Sbjct: 208 HLLGTVSDLKHMARYCDGLLDGNLGLKKLAKLLDVKRIDNAHFACSDNLFTTSVYT 263

[Close]

BLAST of MS027291 vs. ExPASy TrEMBL
Match: A0A6J1HSR3 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111467090 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 3.2e-14
Identity = 43/55 (78.18%), Postives = 49/55 (89.09%), Query Frame = 0


Query: 62  YLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVY 117
           +L+GTVTDLKHMARY D LL+ +LGLKKLAK+L V RIG AHFAGSDSLLTA+VY
Sbjct: 187 HLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSLLTASVY 241

[Close]

BLAST of MS027291 vs. TAIR 10
Match: AT1G06450.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 6.9e-09
Identity = 30/56 (53.57%), Postives = 39/56 (69.64%), Query Frame = 0


Query: 61  IYLIGTVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVY 117
           + + G V D K MA +C+G L   LGL KLA+LL + R+G AH AGSDSL+TA V+
Sbjct: 180 VKVFGQVYDTKVMAGFCEG-LGEHLGLSKLAQLLQITRVGRAHHAGSDSLMTALVF 234

[Close]

BLAST of MS027291 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 2.0e-08
Identity = 27/51 (52.94%), Postives = 38/51 (74.51%), Query Frame = 0


Query: 66  TVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVY 117
           TV D+KH+ ++C+ L     GL KLA+LL+V+R+GI H AGSDSLLT+  +
Sbjct: 196 TVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTF 243

[Close]

BLAST of MS027291 vs. TAIR 10
Match: AT1G80780.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 2.0e-08
Identity = 27/51 (52.94%), Postives = 38/51 (74.51%), Query Frame = 0


Query: 66  TVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVY 117
           TV D+KH+ ++C+ L     GL KLA+LL+V+R+GI H AGSDSLLT+  +
Sbjct: 196 TVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTF 243

[Close]

BLAST of MS027291 vs. TAIR 10
Match: AT1G80780.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 2.0e-08
Identity = 27/51 (52.94%), Postives = 38/51 (74.51%), Query Frame = 0


Query: 66  TVTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVY 117
           TV D+KH+ ++C+ L     GL KLA+LL+V+R+GI H AGSDSLLT+  +
Sbjct: 196 TVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTF 243

[Close]

BLAST of MS027291 vs. TAIR 10
Match: AT2G32070.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 3.4e-08
Identity = 27/50 (54.00%), Postives = 37/50 (74.00%), Query Frame = 0


Query: 67  VTDLKHMARYCDGLLDSDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVY 117
           V D+KH+ ++C+ L     GL KLA+LLDV+R+GI H AGSDSLLT+  +
Sbjct: 198 VYDIKHLMKFCNSLHG---GLNKLAELLDVERVGICHQAGSDSLLTSCTF 244

The following BLAST results are available for this feature:

BLAST of MS027291 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158209.1	9.7e-21	96.43	probable CCR4-associated factor 1 homolog 5 [Momordica charantia]	[more]
XP_022154798.1	2.4e-19	91.07	probable CCR4-associated factor 1 homolog 6 [Momordica charantia]	[more]
XP_038888808.1	1.1e-16	87.04	probable CCR4-associated factor 1 homolog 6 [Benincasa hispida]	[more]
XP_022153955.1	4.6e-15	75.00	putative CCR4-associated factor 1 homolog 8 [Momordica charantia]	[more]
XP_022146384.1	6.1e-15	78.57	putative CCR4-associated factor 1 homolog 8 [Momordica charantia]	[more]

BLAST of MS027291 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SHJ0	9.7e-08	53.57	Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SAI2	2.8e-07	52.94	Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SKZ2	4.8e-07	54.00	Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9LEU4	4.8e-07	50.91	Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9SFX6	4.1e-06	52.94	Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]

BLAST of MS027291 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DYQ7	4.7e-21	96.43	probable CCR4-associated factor 1 homolog 5 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1DLA5	1.2e-19	91.07	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=...	[more]
A0A6J1DIB1	2.2e-15	75.00	putative CCR4-associated factor 1 homolog 8 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1CXZ5	2.9e-15	78.57	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=...	[more]
A0A6J1HSR3	3.2e-14	78.18	Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111467090 PE=3 S...	[more]

BLAST of MS027291 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G06450.1	6.9e-09	53.57	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.1	2.0e-08	52.94	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.2	2.0e-08	52.94	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.3	2.0e-08	52.94	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G32070.1	3.4e-08	54.00	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 13..114 e-value: 2.1E-11 score: 45.4
IPR039637	CCR4-NOT transcription complex subunit 7/8/Pop2	PANTHER	PTHR10797	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT	coord: 60..114
None	No IPR available	PANTHER	PTHR10797:SF57	CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATED	coord: 60..114
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 59..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS027291.1	MS027291.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0030014	CCR4-NOT complex
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004535	poly(A)-specific ribonuclease activity