MS027109 (gene) Bitter gourd (TR) v1

BLAST of MS027109 vs. NCBI nr
Match: XP_022145518.1 (GDSL esterase/lipase CPRD49-like [Momordica charantia])

HSP 1 Score: 468.4 bits (1204), Expect = 3.7e-128
Identity = 239/286 (83.57%), Postives = 239/286 (83.57%), Query Frame = 0

Query: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60
           MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS
Sbjct: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60

Query: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFP------------- 120
           IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFP             
Sbjct: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFPKDSIIQPSLAIVY 120

Query: 121 ----------------------------------KSLSTTTRSIFLTPPPVNSDQIRELL 180
                                             KSLSTTTRSIFLTPPPVNSDQIRELL
Sbjct: 121 FGGNDAIIPLPFPGGSHVPLPQFIDNMTKIACHIKSLSTTTRSIFLTPPPVNSDQIRELL 180

Query: 181 PGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRV 240
           PGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRV
Sbjct: 181 PGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRV 240

BLAST of MS027109 vs. NCBI nr
Match: XP_022145497.1 (GDSL esterase/lipase CPRD49-like [Momordica charantia])

HSP 1 Score: 412.1 bits (1058), Expect = 3.2e-111
Identity = 206/282 (73.05%), Postives = 222/282 (78.72%), Query Frame = 0

Query: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60
           MSCSLLNPL VGS RCQLPKQHPSLRPSLS  FPGALSRCGWR KMVGP+RPKFV+FGSS
Sbjct: 1   MSCSLLNPLPVGSGRCQLPKQHPSLRPSLSTQFPGALSRCGWRTKMVGPMRPKFVVFGSS 60

Query: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFP------------- 120
           IVQ+SF D+GWGA+IANLYARQADVILRGY+GWNSSQAL+VVDEVFP             
Sbjct: 61  IVQFSFADKGWGAEIANLYARQADVILRGYSGWNSSQALEVVDEVFPKDSIIQPSLVIVY 120

Query: 121 ------------------------------KSLSTTTRSIFLTPPPVNSDQIRELLPGLE 180
                                         K+LSTTTR IFLTPPPVNS+QIREL PGLE
Sbjct: 121 FGGNDAVINFPTSVPLSEFTVNMTKIASHIKNLSTTTRGIFLTPPPVNSEQIRELFPGLE 180

Query: 181 KLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRVVFDE 240
           KLRTMENLE YTDALIDIC+EIGVEYVDLFRAVQ+R+DWLTFFTDGVHF E+GS VVF+E
Sbjct: 181 KLRTMENLEKYTDALIDICKEIGVEYVDLFRAVQRRDDWLTFFTDGVHFNEKGSHVVFEE 240

BLAST of MS027109 vs. NCBI nr
Match: XP_022145498.1 (GDSL esterase/lipase CPRD49-like [Momordica charantia])

HSP 1 Score: 409.5 bits (1051), Expect = 2.1e-110
Identity = 209/286 (73.08%), Postives = 222/286 (77.62%), Query Frame = 0

Query: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60
           MSCSLLNP+ VGS RCQLPKQH SLRPSLS HFPGALSR GWRAKMVGPVRPKFVLFGSS
Sbjct: 25  MSCSLLNPVPVGSGRCQLPKQHLSLRPSLSTHFPGALSRHGWRAKMVGPVRPKFVLFGSS 84

Query: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFP------------- 120
           IVQ+SF DRGWGADIA+LY+RQADVILRGY GWNSS+ALQVVDE+FP             
Sbjct: 85  IVQFSFGDRGWGADIADLYSRQADVILRGYGGWNSSRALQVVDEIFPKDSIIQPSLVIVY 144

Query: 121 ----------------------------------KSLSTTTRSIFLTPPPVNSDQIRELL 180
                                             KSLS TTRSIFLTPPPVNS+QIREL+
Sbjct: 145 FGGNDAVIPLPFPGGSHVPLPEFINNMTNIVCHIKSLSATTRSIFLTPPPVNSEQIRELM 204

Query: 181 PGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRV 240
           PGLEKLRTMENLEIYTDA+IDICR+IGVEYVDLFRAVQ+R+DWLTFFTDGVHF EEGSRV
Sbjct: 205 PGLEKLRTMENLEIYTDAIIDICRDIGVEYVDLFRAVQRRDDWLTFFTDGVHFNEEGSRV 264

BLAST of MS027109 vs. NCBI nr
Match: XP_022145517.1 (GDSL esterase/lipase At2g38180-like [Momordica charantia])

HSP 1 Score: 366.7 bits (940), Expect = 1.5e-97
Identity = 178/263 (67.68%), Postives = 201/263 (76.43%), Query Frame = 0

Query: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60
           MSCSLLNPL+VGS RCQLPKQHPS RPSLSIHFP ALSR GWR KMVGPVRPKFV+FGSS
Sbjct: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60

Query: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFPKS----------- 120
           IVQ+SFDD+GWGADIANLYARQADVILRGYAGW+S QAL+VVD+VFPK            
Sbjct: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120

Query: 121 --------------------------------LSTTTRSIFLTPPPVNSDQIRELLPGLE 180
                                           LSTTTRSIFLTPPP+NS+Q+++L+PGLE
Sbjct: 121 FGGNDASINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLMPGLE 180

Query: 181 KLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRVVFDE 221
           KLRTMENLE+Y DALIDIC+++GVEYVDLFRA+Q +EDWLTFF DGVHF E+GSR  FDE
Sbjct: 181 KLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRAAFDE 240

BLAST of MS027109 vs. NCBI nr
Match: XP_022145516.1 (GDSL esterase/lipase CPRD49-like [Momordica charantia])

HSP 1 Score: 365.5 bits (937), Expect = 3.4e-97
Identity = 179/263 (68.06%), Postives = 202/263 (76.81%), Query Frame = 0

Query: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60
           MSCSLLNPLQVGS RCQLPKQHPSLRPSLSI FPGALSR GWR KMVGPVRPKFVLFGSS
Sbjct: 1   MSCSLLNPLQVGSGRCQLPKQHPSLRPSLSIQFPGALSRRGWRTKMVGPVRPKFVLFGSS 60

Query: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFPKS----------- 120
           IVQ+SFDD+GWGADIANLYARQADVILRGYAGW+SSQA+QVVD++FPK            
Sbjct: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSSQAIQVVDKIFPKDSTTQPALVIVY 120

Query: 121 --------------------------------LSTTTRSIFLTPPPVNSDQIRELLPGLE 180
                                           LSTTTRSIFLTPPPVNS+Q+++ +PGLE
Sbjct: 121 FGGNDAAINFPNSVPVDEYIDNMTKIAEHITCLSTTTRSIFLTPPPVNSEQLKQQMPGLE 180

Query: 181 KLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRVVFDE 221
           K+RTMENLEIY+DALID+CR+IGVEY+DLFR +Q+ +DWLTFF DGVHF E+GSRVVFDE
Sbjct: 181 KVRTMENLEIYSDALIDVCRKIGVEYIDLFRVIQRNDDWLTFFRDGVHFNEKGSRVVFDE 240

BLAST of MS027109 vs. ExPASy Swiss-Prot
Match: O80443 (GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.4e-45
Identity = 103/240 (42.92%), Postives = 136/240 (56.67%), Query Frame = 0

Query: 46  MVGPVRPKFVLFGSSIVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEV 105
           MVGPVRP+ VLFGSSIVQYSF DRGWGA +A+LY+R AD+ILRGYAGWNS  AL+V+ +V
Sbjct: 1   MVGPVRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQV 60

Query: 106 FPK-----------------------------------------------SLSTTTRSIF 165
           FPK                                               SLS  TR IF
Sbjct: 61  FPKDAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIF 120

Query: 166 LTPPPVNSDQIRELLPGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWL- 225
           LTPPP+N  QI  +     K R+ E    Y + L+++CREI V+ +D++ A+Q+++DWL 
Sbjct: 121 LTPPPMNEKQIEIVFGDAIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDDWLN 180

Query: 226 TFFTDGVHFTEEGSRVVFDEIKNTINRANWVPSLAVGSMPIEFGNNTGLRSFDFEGSSII 238
           + FTDG+HFT + S +V  EI   +  A+W PSL   S+P+EF        FDF+  + I
Sbjct: 181 SCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEF-------PFDFDAPNSI 233

BLAST of MS027109 vs. ExPASy Swiss-Prot
Match: Q9SRM5 (GDSL esterase/lipase CPRD49 OS=Arabidopsis thaliana OX=3702 GN=CPRD49 PE=2 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 8.5e-43
Identity = 98/229 (42.79%), Postives = 132/229 (57.64%), Query Frame = 0

Query: 46  MVGPVRPKFVLFGSSIVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEV 105
           MVGP RP+ VLFGSSIVQ SF   GWGA ++ +YAR+AD+ILRGY GWNSS+AL+VVD+V
Sbjct: 1   MVGPARPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQV 60

Query: 106 FPK-----------------------------------------------SLSTTTRSIF 165
           FPK                                               SLS  TR IF
Sbjct: 61  FPKDAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIF 120

Query: 166 LTPPPVNSDQIRE-LLPGL-EKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDW 225
           L+ PPV+  ++R+   P L E +RT +  + Y+DA +++C+E+G+E VDLF   QK +DW
Sbjct: 121 LSSPPVDEAKVRQNQSPYLSEVIRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKADDW 180

BLAST of MS027109 vs. ExPASy Swiss-Prot
Match: Q9FM04 (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 102.1 bits (253), Expect = 9.2e-21
Identity = 66/230 (28.70%), Postives = 104/230 (45.22%), Query Frame = 0

Query: 50  VRPKFVLFGSSIVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFP-- 109
           +RP+ VLFG SI   SF   GWG+ +A+ Y+R+ADV++RGY G+N+  AL ++  +FP  
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 110 ---------------------------------------------KSLSTTTRSIFLTPP 169
                                                        K  S T   + +TPP
Sbjct: 61  SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPP 120

Query: 170 PVNS---DQIRELLPG----LEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKRED 225
           P++        E + G     E  RT E   +Y    + +  E+G+  V+L+  +Q+  D
Sbjct: 121 PIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETND 180

BLAST of MS027109 vs. ExPASy Swiss-Prot
Match: Q6NMR9 (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.3e-18
Identity = 62/205 (30.24%), Postives = 94/205 (45.85%), Query Frame = 0

Query: 50  VRPKFVLFGSSIVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFP-- 109
           +R K  LFG SI + SF D GWGA +A+L  R+AD++LRGY+G+N+  AL+VV+ VFP  
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 110 ------------------------------------------------KSLSTTTRSIFL 169
                                                           K+    T  I +
Sbjct: 62  EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILI 121

Query: 170 TPPPVNSDQIRELLPGLEKL-----RTMENLEIYTDALIDICREIGVEYVDLFRAVQKRE 199
           TPPP++ ++ R   P +E       RT E   +Y  A I +  E  +   DL+  +Q+  
Sbjct: 122 TPPPID-EEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQIP 181

BLAST of MS027109 vs. ExPASy Swiss-Prot
Match: P41734 (Isoamyl acetate-hydrolyzing esterase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=IAH1 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.3e-14
Identity = 60/203 (29.56%), Postives = 92/203 (45.32%), Query Frame = 0

Query: 53  KFVLFGSSIVQYSFDDR---------GWGADIANLYARQADVILRGYAGWNSSQALQVVD 112
           KF+LFG SI +++F+ R           GA + N Y R+ D++ RG+ G+ S  AL+++ 
Sbjct: 5   KFLLFGDSITEFAFNTRPIEDGKDQYALGAALVNEYTRKMDILQRGFKGYTSRWALKILP 64

Query: 113 EVFPKSLSTTTRSIFL------------TPPPVNSDQIRELL--------------PGL- 172
           E+     +    +IFL             P P   D IR+++              PGL 
Sbjct: 65  EILKHESNIVMATIFLGANDACSAGPQSVPLPEFIDNIRQMVSLMKSYHIRPIIIGPGLV 124

Query: 173 -------EK--------LRTMENLEIYTDALIDICREIGVEYVDLFRAVQKR--EDWLTF 203
                  EK         RT EN  IY+DAL  +  E  V +V L +A Q+   + W   
Sbjct: 125 DREKWEKEKSEEIALGYFRTNENFAIYSDALAKLANEEKVPFVALNKAFQQEGGDAWQQL 184

BLAST of MS027109 vs. ExPASy TrEMBL
Match: A0A6J1CW58 (GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014947 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 1.8e-128
Identity = 239/286 (83.57%), Postives = 239/286 (83.57%), Query Frame = 0

Query: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60
           MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS
Sbjct: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60

Query: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFP------------- 120
           IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFP             
Sbjct: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFPKDSIIQPSLAIVY 120

Query: 121 ----------------------------------KSLSTTTRSIFLTPPPVNSDQIRELL 180
                                             KSLSTTTRSIFLTPPPVNSDQIRELL
Sbjct: 121 FGGNDAIIPLPFPGGSHVPLPQFIDNMTKIACHIKSLSTTTRSIFLTPPPVNSDQIRELL 180

Query: 181 PGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRV 240
           PGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRV
Sbjct: 181 PGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRV 240

BLAST of MS027109 vs. ExPASy TrEMBL
Match: A0A6J1CWS0 (GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014934 PE=4 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.5e-111
Identity = 206/282 (73.05%), Postives = 222/282 (78.72%), Query Frame = 0

Query: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60
           MSCSLLNPL VGS RCQLPKQHPSLRPSLS  FPGALSRCGWR KMVGP+RPKFV+FGSS
Sbjct: 1   MSCSLLNPLPVGSGRCQLPKQHPSLRPSLSTQFPGALSRCGWRTKMVGPMRPKFVVFGSS 60

Query: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFP------------- 120
           IVQ+SF D+GWGA+IANLYARQADVILRGY+GWNSSQAL+VVDEVFP             
Sbjct: 61  IVQFSFADKGWGAEIANLYARQADVILRGYSGWNSSQALEVVDEVFPKDSIIQPSLVIVY 120

Query: 121 ------------------------------KSLSTTTRSIFLTPPPVNSDQIRELLPGLE 180
                                         K+LSTTTR IFLTPPPVNS+QIREL PGLE
Sbjct: 121 FGGNDAVINFPTSVPLSEFTVNMTKIASHIKNLSTTTRGIFLTPPPVNSEQIRELFPGLE 180

Query: 181 KLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRVVFDE 240
           KLRTMENLE YTDALIDIC+EIGVEYVDLFRAVQ+R+DWLTFFTDGVHF E+GS VVF+E
Sbjct: 181 KLRTMENLEKYTDALIDICKEIGVEYVDLFRAVQRRDDWLTFFTDGVHFNEKGSHVVFEE 240

BLAST of MS027109 vs. ExPASy TrEMBL
Match: A0A6J1CW36 (GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014935 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 9.9e-111
Identity = 209/286 (73.08%), Postives = 222/286 (77.62%), Query Frame = 0

Query: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60
           MSCSLLNP+ VGS RCQLPKQH SLRPSLS HFPGALSR GWRAKMVGPVRPKFVLFGSS
Sbjct: 25  MSCSLLNPVPVGSGRCQLPKQHLSLRPSLSTHFPGALSRHGWRAKMVGPVRPKFVLFGSS 84

Query: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFP------------- 120
           IVQ+SF DRGWGADIA+LY+RQADVILRGY GWNSS+ALQVVDE+FP             
Sbjct: 85  IVQFSFGDRGWGADIADLYSRQADVILRGYGGWNSSRALQVVDEIFPKDSIIQPSLVIVY 144

Query: 121 ----------------------------------KSLSTTTRSIFLTPPPVNSDQIRELL 180
                                             KSLS TTRSIFLTPPPVNS+QIREL+
Sbjct: 145 FGGNDAVIPLPFPGGSHVPLPEFINNMTNIVCHIKSLSATTRSIFLTPPPVNSEQIRELM 204

Query: 181 PGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRV 240
           PGLEKLRTMENLEIYTDA+IDICR+IGVEYVDLFRAVQ+R+DWLTFFTDGVHF EEGSRV
Sbjct: 205 PGLEKLRTMENLEIYTDAIIDICRDIGVEYVDLFRAVQRRDDWLTFFTDGVHFNEEGSRV 264

BLAST of MS027109 vs. ExPASy TrEMBL
Match: A0A6J1CWU2 (GDSL esterase/lipase At2g38180-like OS=Momordica charantia OX=3673 GN=LOC111014946 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 7.4e-98
Identity = 178/263 (67.68%), Postives = 201/263 (76.43%), Query Frame = 0

Query: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60
           MSCSLLNPL+VGS RCQLPKQHPS RPSLSIHFP ALSR GWR KMVGPVRPKFV+FGSS
Sbjct: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60

Query: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFPKS----------- 120
           IVQ+SFDD+GWGADIANLYARQADVILRGYAGW+S QAL+VVD+VFPK            
Sbjct: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120

Query: 121 --------------------------------LSTTTRSIFLTPPPVNSDQIRELLPGLE 180
                                           LSTTTRSIFLTPPP+NS+Q+++L+PGLE
Sbjct: 121 FGGNDASINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLMPGLE 180

Query: 181 KLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRVVFDE 221
           KLRTMENLE+Y DALIDIC+++GVEYVDLFRA+Q +EDWLTFF DGVHF E+GSR  FDE
Sbjct: 181 KLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRAAFDE 240

BLAST of MS027109 vs. ExPASy TrEMBL
Match: A0A6J1CWJ2 (GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014945 PE=4 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.6e-97
Identity = 179/263 (68.06%), Postives = 202/263 (76.81%), Query Frame = 0

Query: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60
           MSCSLLNPLQVGS RCQLPKQHPSLRPSLSI FPGALSR GWR KMVGPVRPKFVLFGSS
Sbjct: 1   MSCSLLNPLQVGSGRCQLPKQHPSLRPSLSIQFPGALSRRGWRTKMVGPVRPKFVLFGSS 60

Query: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFPKS----------- 120
           IVQ+SFDD+GWGADIANLYARQADVILRGYAGW+SSQA+QVVD++FPK            
Sbjct: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSSQAIQVVDKIFPKDSTTQPALVIVY 120

Query: 121 --------------------------------LSTTTRSIFLTPPPVNSDQIRELLPGLE 180
                                           LSTTTRSIFLTPPPVNS+Q+++ +PGLE
Sbjct: 121 FGGNDAAINFPNSVPVDEYIDNMTKIAEHITCLSTTTRSIFLTPPPVNSEQLKQQMPGLE 180

Query: 181 KLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRVVFDE 221
           K+RTMENLEIY+DALID+CR+IGVEY+DLFR +Q+ +DWLTFF DGVHF E+GSRVVFDE
Sbjct: 181 KVRTMENLEIYSDALIDVCRKIGVEYIDLFRVIQRNDDWLTFFRDGVHFNEKGSRVVFDE 240

BLAST of MS027109 vs. TAIR 10
Match: AT2G38180.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 184.5 bits (467), Expect = 1.0e-46
Identity = 103/240 (42.92%), Postives = 136/240 (56.67%), Query Frame = 0

Query: 46  MVGPVRPKFVLFGSSIVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEV 105
           MVGPVRP+ VLFGSSIVQYSF DRGWGA +A+LY+R AD+ILRGYAGWNS  AL+V+ +V
Sbjct: 1   MVGPVRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQV 60

Query: 106 FPK-----------------------------------------------SLSTTTRSIF 165
           FPK                                               SLS  TR IF
Sbjct: 61  FPKDAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIF 120

Query: 166 LTPPPVNSDQIRELLPGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWL- 225
           LTPPP+N  QI  +     K R+ E    Y + L+++CREI V+ +D++ A+Q+++DWL 
Sbjct: 121 LTPPPMNEKQIEIVFGDAIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDDWLN 180

Query: 226 TFFTDGVHFTEEGSRVVFDEIKNTINRANWVPSLAVGSMPIEFGNNTGLRSFDFEGSSII 238
           + FTDG+HFT + S +V  EI   +  A+W PSL   S+P+EF        FDF+  + I
Sbjct: 181 SCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEF-------PFDFDAPNSI 233

BLAST of MS027109 vs. TAIR 10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 175.3 bits (443), Expect = 6.1e-44
Identity = 98/229 (42.79%), Postives = 132/229 (57.64%), Query Frame = 0

Query: 46  MVGPVRPKFVLFGSSIVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEV 105
           MVGP RP+ VLFGSSIVQ SF   GWGA ++ +YAR+AD+ILRGY GWNSS+AL+VVD+V
Sbjct: 1   MVGPARPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQV 60

Query: 106 FPK-----------------------------------------------SLSTTTRSIF 165
           FPK                                               SLS  TR IF
Sbjct: 61  FPKDAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIF 120

Query: 166 LTPPPVNSDQIRE-LLPGL-EKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDW 225
           L+ PPV+  ++R+   P L E +RT +  + Y+DA +++C+E+G+E VDLF   QK +DW
Sbjct: 121 LSSPPVDEAKVRQNQSPYLSEVIRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKADDW 180

BLAST of MS027109 vs. TAIR 10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 102.1 bits (253), Expect = 6.5e-22
Identity = 66/230 (28.70%), Postives = 104/230 (45.22%), Query Frame = 0

Query: 50  VRPKFVLFGSSIVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFP-- 109
           +RP+ VLFG SI   SF   GWG+ +A+ Y+R+ADV++RGY G+N+  AL ++  +FP  
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 110 ---------------------------------------------KSLSTTTRSIFLTPP 169
                                                        K  S T   + +TPP
Sbjct: 61  SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPP 120

Query: 170 PVNS---DQIRELLPG----LEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKRED 225
           P++        E + G     E  RT E   +Y    + +  E+G+  V+L+  +Q+  D
Sbjct: 121 PIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETND 180

BLAST of MS027109 vs. TAIR 10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 93.2 bits (230), Expect = 3.0e-19
Identity = 62/205 (30.24%), Postives = 94/205 (45.85%), Query Frame = 0

Query: 50  VRPKFVLFGSSIVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFP-- 109
           +R K  LFG SI + SF D GWGA +A+L  R+AD++LRGY+G+N+  AL+VV+ VFP  
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 110 ------------------------------------------------KSLSTTTRSIFL 169
                                                           K+    T  I +
Sbjct: 62  EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILI 121

Query: 170 TPPPVNSDQIRELLPGLEKL-----RTMENLEIYTDALIDICREIGVEYVDLFRAVQKRE 199
           TPPP++ ++ R   P +E       RT E   +Y  A I +  E  +   DL+  +Q+  
Sbjct: 122 TPPPID-EEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQIP 181