MS026939 (gene) Bitter gourd (TR) v1

BLAST of MS026939 vs. NCBI nr
Match: XP_022152675.1 (ribonuclease MC [Momordica charantia])

HSP 1 Score: 459.5 bits (1181), Expect = 1.6e-125
Identity = 217/218 (99.54%), Postives = 217/218 (99.54%), Query Frame = 0

Query: 1   MRAMGVPKREVAISLALTIFSLMAFSDAFDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFT 60
           MRAMGVPKREVAISLALTIFSLMA SDAFDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFT
Sbjct: 1   MRAMGVPKREVAISLALTIFSLMALSDAFDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFT 60

Query: 61  IHGLWPQQSGTSLTNCPGSPFDITKISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCS 120
           IHGLWPQQSGTSLTNCPGSPFDITKISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCS
Sbjct: 61  IHGLWPQQSGTSLTNCPGSPFDITKISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCS 120

Query: 121 ESTFNQAAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCR 180
           ESTFNQAAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCR
Sbjct: 121 ESTFNQAAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCR 180

Query: 181 TDPQTKVSYLVEVVACFAQDGSTLIDCTRDTCGANFIF 219
           TDPQTKVSYLVEVVACFAQDGSTLIDCTRDTCGANFIF
Sbjct: 181 TDPQTKVSYLVEVVACFAQDGSTLIDCTRDTCGANFIF 218

BLAST of MS026939 vs. NCBI nr
Match: AKU36755.1 (ribonuclease MC1, partial [synthetic construct] >1BK7_A RIBONUCLEASE MC1 FROM THE SEEDS OF BITTER GOURD [Momordica charantia] >1UCA_A Crystal structure of the Ribonuclease MC1 from bitter gourd seeds complexed with 2'-UMP [Momordica charantia] >1UCC_A Crystal structure of the Ribonuclease MC1 from bitter gourd seeds complexed with 3'-UMP. [Momordica charantia] >1UCD_A Crystal structure of Ribonuclease MC1 from bitter gourd seeds complexed with 5'-UMP [Momordica charantia])

HSP 1 Score: 411.0 bits (1055), Expect = 6.4e-111
Identity = 189/190 (99.47%), Postives = 190/190 (100.00%), Query Frame = 0

Query: 29  FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCPGSPFDITKISH 88
           FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCPGSPFDITKISH
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCPGSPFDITKISH 60

Query: 89  LQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALRP 148
           LQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALRP
Sbjct: 61  LQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALRP 120

Query: 149 HAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEVVACFAQDGSTLIDCT 208
           HAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLV+VVACFAQDGSTLIDCT
Sbjct: 121 HAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDCT 180

Query: 209 RDTCGANFIF 219
           RDTCGANFIF
Sbjct: 181 RDTCGANFIF 190

BLAST of MS026939 vs. NCBI nr
Match: 1J1F_A (Crystal structure of the RNase MC1 mutant N71T in complex with 5'-GMP [Momordica charantia])

HSP 1 Score: 409.8 bits (1052), Expect = 1.4e-110
Identity = 189/190 (99.47%), Postives = 189/190 (99.47%), Query Frame = 0

Query: 29  FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCPGSPFDITKISH 88
           FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCPGSPFDITKISH
Sbjct: 2   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCPGSPFDITKISH 61

Query: 89  LQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALRP 148
           LQSQLNTLWP VLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALRP
Sbjct: 62  LQSQLNTLWPTVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALRP 121

Query: 149 HAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEVVACFAQDGSTLIDCT 208
           HAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEVVACFAQDGSTLIDCT
Sbjct: 122 HAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEVVACFAQDGSTLIDCT 181

Query: 209 RDTCGANFIF 219
           RDTCGANFIF
Sbjct: 182 RDTCGANFIF 191

BLAST of MS026939 vs. NCBI nr
Match: 1V9H_A (Crystal structure of the RNase MC1 mutant Y101A in complex with 5'-UMP [Momordica charantia])

HSP 1 Score: 408.7 bits (1049), Expect = 3.2e-110
Identity = 189/190 (99.47%), Postives = 189/190 (99.47%), Query Frame = 0

Query: 29  FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCPGSPFDITKISH 88
           FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCPGSPFDITKISH
Sbjct: 8   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCPGSPFDITKISH 67

Query: 89  LQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALRP 148
           LQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAA FKLAVDMRNNYDIIGALRP
Sbjct: 68  LQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAAFKLAVDMRNNYDIIGALRP 127

Query: 149 HAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEVVACFAQDGSTLIDCT 208
           HAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEVVACFAQDGSTLIDCT
Sbjct: 128 HAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEVVACFAQDGSTLIDCT 187

Query: 209 RDTCGANFIF 219
           RDTCGANFIF
Sbjct: 188 RDTCGANFIF 197

BLAST of MS026939 vs. NCBI nr
Match: 1J1G_A (Crystal structure of the RNase MC1 mutant N71S in complex with 5'-GMP [Momordica charantia])

HSP 1 Score: 408.3 bits (1048), Expect = 4.2e-110
Identity = 188/189 (99.47%), Postives = 189/189 (100.00%), Query Frame = 0

Query: 30  DSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCPGSPFDITKISHL 89
           DSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCPGSPFDITKISHL
Sbjct: 2   DSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCPGSPFDITKISHL 61

Query: 90  QSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALRPH 149
           QSQLNTLWP+VLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALRPH
Sbjct: 62  QSQLNTLWPSVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALRPH 121

Query: 150 AAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEVVACFAQDGSTLIDCTR 209
           AAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEVVACFAQDGSTLIDCTR
Sbjct: 122 AAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEVVACFAQDGSTLIDCTR 181

Query: 210 DTCGANFIF 219
           DTCGANFIF
Sbjct: 182 DTCGANFIF 190

BLAST of MS026939 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 1.3e-111
Identity = 188/191 (98.43%), Postives = 189/191 (98.95%), Query Frame = 0

Query: 29  FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNCP-GSPFDITKIS 88
           FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQ SGTSLTNCP GSPFDITKIS
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 89  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 148
           HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 120

Query: 149 PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEVVACFAQDGSTLIDC 208
           PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLV+VVACFAQDGSTLIDC
Sbjct: 121 PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDC 180

Query: 209 TRDTCGANFIF 219
           TRDTCGANFIF
Sbjct: 181 TRDTCGANFIF 191

BLAST of MS026939 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.3e-34
Identity = 80/210 (38.10%), Postives = 114/210 (54.29%), Query Frame = 0

Query: 18  TIFSLMAFSDAFDSFWFVQQWPPAVCSFQKSGSCPGSG--LRTFTIHGLWPQ-QSGTSLT 77
           ++FS  + S+ FD F+FVQQWP + C  QK    P SG     F IHGLWP  + GT  +
Sbjct: 19  SVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPS 78

Query: 78  NCPGS-PFDITKISHLQSQLNTLWPNVL--RANNQQFWSHEWTKHGTCSESTFNQAAYFK 137
           NC  S PFD + IS L + +   WP +     + + FW HEW KHGTCSES  +Q  YF+
Sbjct: 79  NCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQ 138

Query: 138 LAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLV 197
            A++++   +++GAL      P+G++ S ++I+  +K   G  P + C  D     S L 
Sbjct: 139 TALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGN-SQLY 198

Query: 198 EVVACFAQDGSTLIDC---TRDTCGANFIF 219
           +V  C  + GS LI+C       CGA   F
Sbjct: 199 QVYLCVDRSGSGLIECPVFPHGKCGAEIEF 227

BLAST of MS026939 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 140.2 bits (352), Expect = 2.8e-32
Identity = 77/194 (39.69%), Postives = 111/194 (57.22%), Query Frame = 0

Query: 21  SLMAFSDAFDSFWFVQQWPPAVCSFQKSGSCPGSG--LRTFTIHGLWPQQS-GTSLTNC- 80
           S++  +  FD F+FVQQWP + C  ++S   P +G     F IHGLWP  + GT  +NC 
Sbjct: 21  SVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWPNNNDGTYPSNCD 80

Query: 81  PGSPFDITKISHLQSQLNTLWPNVL--RANNQQFWSHEWTKHGTCSESTF-NQAAYFKLA 140
           P SP+D ++IS L S +   WP +     +   FWSHEW KHGTC+ES   NQ AYFK A
Sbjct: 81  PNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESVLTNQHAYFKKA 140

Query: 141 VDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEV 200
           +D++N  D++  L+     P+G +     I+  +K+  G  P ++C  D Q+  S L +V
Sbjct: 141 LDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPWIQCNVD-QSGNSQLYQV 200

Query: 201 VACFAQDGSTLIDC 208
             C    GS+LI+C
Sbjct: 201 YICVDGSGSSLIEC 213

BLAST of MS026939 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.0e-26
Identity = 69/199 (34.67%), Postives = 102/199 (51.26%), Query Frame = 0

Query: 15  LALTIFSLMAFSDAFDSFWFVQQWPPAVCSFQKSGSCPGSG--LRTFTIHGLWPQ-QSGT 74
           LAL    + +F+  FD F+FV QWP A C  + S   P +G     F IHGLWP  ++G 
Sbjct: 8   LALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGG 67

Query: 75  SLTNC-PGSPFDITKISHLQSQLNTLWPNVLRANN--QQFWSHEWTKHGTCSESTFNQAA 134
              NC P S FD  ++S L S L   WP +   +N   +FW+HEW KHGTC+ES  +Q  
Sbjct: 68  WPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHD 127

Query: 135 YFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVS 194
           YF+  + ++   +++ AL      P+ +    + I+  +K   G  PG+ C  D  +  S
Sbjct: 128 YFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKD-SSHNS 187

Query: 195 YLVEVVACFAQDGSTLIDC 208
            L ++  C     S  I+C
Sbjct: 188 QLYQIYLCVDTSASKFINC 205

BLAST of MS026939 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 2.4e-23
Identity = 70/204 (34.31%), Postives = 100/204 (49.02%), Query Frame = 0

Query: 11  VAISLALTIFSLMAFSDAFDSFWFVQQWPPAVCSFQKSGSCPGSGL--RTFTIHGLWPQ- 70
           + I +   +  L   S  FD F+FVQQWP + C  ++S   P +G     F+IHGLWP  
Sbjct: 10  IKIIVVQCLLVLCVTSQDFDFFYFVQQWPASYCDTRRSCCYPTTGKPDEDFSIHGLWPNY 69

Query: 71  QSGTSLTNCP-GSPFDITKISHLQSQLNTLWPNVL--RANNQQFWSHEWTKHGTCSESTF 130
           + G    NC   S  D ++ S L S +   WP++    ++  +FWSHEW KHGTC  S  
Sbjct: 70  KDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGTC--SAL 129

Query: 131 NQAAYFKLAVDMRNNYDIIGALRPHAAGP-NGRTKSRQAIKGFLKAKFGKFPGLRCRTDP 190
           NQ AYF+ A+D +   +++  L      P NG     ++IK  ++   G  P + C  D 
Sbjct: 130 NQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPFIECNVDS 189

Query: 191 QTKVSYLVEVVACFAQDGSTLIDC 208
           Q     L +V  C     S  IDC
Sbjct: 190 QGN-HQLYQVYLCVDSSASKFIDC 210

BLAST of MS026939 vs. ExPASy TrEMBL
Match: A0A6J1DEL7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111020337 PE=3 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 7.6e-126
Identity = 217/218 (99.54%), Postives = 217/218 (99.54%), Query Frame = 0

Query: 1   MRAMGVPKREVAISLALTIFSLMAFSDAFDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFT 60
           MRAMGVPKREVAISLALTIFSLMA SDAFDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFT
Sbjct: 1   MRAMGVPKREVAISLALTIFSLMALSDAFDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFT 60

Query: 61  IHGLWPQQSGTSLTNCPGSPFDITKISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCS 120
           IHGLWPQQSGTSLTNCPGSPFDITKISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCS
Sbjct: 61  IHGLWPQQSGTSLTNCPGSPFDITKISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCS 120

Query: 121 ESTFNQAAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCR 180
           ESTFNQAAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCR
Sbjct: 121 ESTFNQAAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCR 180

Query: 181 TDPQTKVSYLVEVVACFAQDGSTLIDCTRDTCGANFIF 219
           TDPQTKVSYLVEVVACFAQDGSTLIDCTRDTCGANFIF
Sbjct: 181 TDPQTKVSYLVEVVACFAQDGSTLIDCTRDTCGANFIF 218

BLAST of MS026939 vs. ExPASy TrEMBL
Match: A0A6J1CKH7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111012440 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 2.2e-64
Identity = 120/221 (54.30%), Postives = 157/221 (71.04%), Query Frame = 0

Query: 2   RAMGVPKREVAI--SLALTIFSLMAFSDAFDSFWFVQQWPPAVCSFQKSGSCPGSGLRTF 61
           R M + KREV +  +L +TIF  +  S AFD+FWFVQQWPPAVC+FQ+ G C G GLRTF
Sbjct: 3   REMVISKREVVLMFALTITIFFPVVKSQAFDNFWFVQQWPPAVCNFQQ-GRCVGQGLRTF 62

Query: 62  TIHGLWPQQSGTSLTNCPGSPFDITKISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTC 121
           T+HGLWPQ+ GTS+ NC G+ FD T++SHLQ+ LN +WPNV+  NN+ FW HEW KHG C
Sbjct: 63  TVHGLWPQKGGTSVINCSGTRFDFTQVSHLQNDLNRVWPNVITGNNRFFWGHEWNKHGIC 122

Query: 122 SESTFNQAAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRC 181
           SES F+  AYF++A++MR++ D++ ALR     PNGRTKSRQ ++  ++A+FGK P LRC
Sbjct: 123 SESKFDMKAYFQMAINMRDSLDLMNALRVGGLAPNGRTKSRQRVQSAIRAQFGKEPVLRC 182

Query: 182 RTDPQTKVSYLVEVVACFAQDGSTLIDC--TRDTCGANFIF 219
           R   +     L+E+V CF  DG TLI+C      C  +FIF
Sbjct: 183 RGTGRN--VRLIEIVMCFDDDGVTLINCNPASSNCANSFIF 220

BLAST of MS026939 vs. ExPASy TrEMBL
Match: Q40115 (Ribonuclease (RNase LC1) OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 9.2e-63
Identity = 118/217 (54.38%), Postives = 149/217 (68.66%), Query Frame = 0

Query: 4   MGVPKREVAISLALTIFSLMAFSDAFDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHG 63
           M + KRE+ +   LTI   M  S  FDSFW VQ WPPAVCSFQ+ G C G GLR+FTIHG
Sbjct: 1   MAMAKREIVLVFVLTILFPMVKSQTFDSFWMVQHWPPAVCSFQQ-GRCVGQGLRSFTIHG 60

Query: 64  LWPQQSGTSLTNCPGSPFDITKISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSEST 123
           +WPQ+ GTS+ NCPG  FD TKISHL+S LN  WPNV+  NN+ FW HEW KHG CS S 
Sbjct: 61  VWPQKGGTSVINCPGPTFDFTKISHLESTLNVDWPNVITGNNKWFWGHEWNKHGICSVSK 120

Query: 124 FNQAAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDP 183
           F+Q AYF++A++MRN+ D++ ALR     PNGR+K+RQ ++  ++A+ GK P LRCR   
Sbjct: 121 FDQQAYFQMAINMRNSIDLLSALRVGGVVPNGRSKARQRVQSAIRAQLGKEPVLRCR--G 180

Query: 184 QTKVSYLVEVVACFAQDGSTLIDC--TRDTCGANFIF 219
             + S L+E+V CF  DG TLI+C      C  +FIF
Sbjct: 181 TGRQSRLLEIVMCFDDDGVTLINCNPANSNCPNSFIF 214

BLAST of MS026939 vs. ExPASy TrEMBL
Match: A0A6J1DQN7 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.2e-62
Identity = 118/219 (53.88%), Postives = 150/219 (68.49%), Query Frame = 0

Query: 4   MGVPKREVAIS--LALTIFSLMAFSDAFDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTI 63
           M   +RE+     LA+TIF  +  S  FD FWFVQQWPPAVCSFQ+ G C G GLRTFTI
Sbjct: 1   MKTSRREIVFMFVLAITIFLPIVKSQTFDDFWFVQQWPPAVCSFQQ-GRCVGQGLRTFTI 60

Query: 64  HGLWPQQSGTSLTNCPGSPFDITKISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSE 123
           HG+WPQ+ GTS+ NCPG+PFD TKIS L++ LN +WPNV+  +N+ FWSHEW KHG CSE
Sbjct: 61  HGVWPQKGGTSVVNCPGTPFDFTKISSLETDLNRVWPNVITGDNKFFWSHEWNKHGVCSE 120

Query: 124 STFNQAAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRT 183
           S FN  AYF++A++MRN+ D++ AL+     PNG +KS+Q ++  L+  FGK P LRCR 
Sbjct: 121 SKFNMKAYFQMAINMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRG 180

Query: 184 DPQTKVSYLVEVVACFAQDGSTLIDC--TRDTCGANFIF 219
                   L+E+V CF  DG TLI+C      C  +FIF
Sbjct: 181 TGSN--IRLIEIVMCFDDDGVTLINCNPVSSNCANSFIF 216

BLAST of MS026939 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 6.8e-58
Identity = 107/204 (52.45%), Postives = 139/204 (68.14%), Query Frame = 0

Query: 17  LTIFSLMAFSDAFDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQQSGTSLTNC 76
           LTI   +  S  FD FWFVQQWPPAVC+ Q SG C G G R+FTIHGLWPQ+ G S+TNC
Sbjct: 16  LTILFPIVKSQTFDDFWFVQQWPPAVCTLQ-SGRCVGRGTRSFTIHGLWPQKGGRSVTNC 75

Query: 77  PGSPFDITKISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDM 136
            G+ FD TKI+HL++ LN +WPNV+  NN+ FW HEW KHG CSES F++A YF+ A++M
Sbjct: 76  TGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYFQTAINM 135

Query: 137 RNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVEVVAC 196
           R+  D++  LR    GPNG +K++Q ++  + + FGK P LRC+     +V  L E+V C
Sbjct: 136 RHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQV-LLTEIVMC 195

Query: 197 FAQDGSTLIDC--TRDTCGANFIF 219
           F  DG TLI+C   R  C  +FIF
Sbjct: 196 FDDDGVTLINCNKARSNCAGSFIF 217

BLAST of MS026939 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 147.9 bits (372), Expect = 9.5e-36
Identity = 80/210 (38.10%), Postives = 114/210 (54.29%), Query Frame = 0

Query: 18  TIFSLMAFSDAFDSFWFVQQWPPAVCSFQKSGSCPGSG--LRTFTIHGLWPQ-QSGTSLT 77
           ++FS  + S+ FD F+FVQQWP + C  QK    P SG     F IHGLWP  + GT  +
Sbjct: 19  SVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPS 78

Query: 78  NCPGS-PFDITKISHLQSQLNTLWPNVL--RANNQQFWSHEWTKHGTCSESTFNQAAYFK 137
           NC  S PFD + IS L + +   WP +     + + FW HEW KHGTCSES  +Q  YF+
Sbjct: 79  NCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQ 138

Query: 138 LAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLV 197
            A++++   +++GAL      P+G++ S ++I+  +K   G  P + C  D     S L 
Sbjct: 139 TALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGN-SQLY 198

Query: 198 EVVACFAQDGSTLIDC---TRDTCGANFIF 219
           +V  C  + GS LI+C       CGA   F
Sbjct: 199 QVYLCVDRSGSGLIECPVFPHGKCGAEIEF 227

BLAST of MS026939 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 125.6 bits (314), Expect = 5.0e-29
Identity = 76/220 (34.55%), Postives = 112/220 (50.91%), Query Frame = 0

Query: 11  VAISLALTIFSLMAFS---DAFDSFWFVQQWPPAVCSFQKSGSCPGSG--LRTFTIHGLW 70
           V + L L +F  +  S   + FD F+FV QWP A C   ++   P SG     F IHGLW
Sbjct: 7   VNVLLKLLVFQGLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLW 66

Query: 71  PQQSGTSL-TNC-PGSPFDITKISHLQSQLNTLWPNVLRANNQ--QFWSHEWTKHGTCSE 130
           P  +G S  +NC P S FD ++IS L S L   WP +   +N+   FW HEW KHGTCSE
Sbjct: 67  PNYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSE 126

Query: 131 STFNQAAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRT 190
           S  +Q  YF+ A+ ++   +++  L+     P+    +   I   +K   G  PG+ C  
Sbjct: 127 SVMDQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNK 186

Query: 191 DPQTKVSYLVEVVACFAQDGSTLIDC---TRDTCGANFIF 219
           DP+ + + L ++  C    G+  I+C    R +C +   F
Sbjct: 187 DPE-RNAQLHQIYICVDTSGTEFIECPVLPRGSCPSQIQF 225

BLAST of MS026939 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 120.2 bits (300), Expect = 2.1e-27
Identity = 69/199 (34.67%), Postives = 102/199 (51.26%), Query Frame = 0

Query: 15  LALTIFSLMAFSDAFDSFWFVQQWPPAVCSFQKSGSCPGSG--LRTFTIHGLWPQ-QSGT 74
           LAL    + +F+  FD F+FV QWP A C  + S   P +G     F IHGLWP  ++G 
Sbjct: 8   LALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGG 67

Query: 75  SLTNC-PGSPFDITKISHLQSQLNTLWPNVLRANN--QQFWSHEWTKHGTCSESTFNQAA 134
              NC P S FD  ++S L S L   WP +   +N   +FW+HEW KHGTC+ES  +Q  
Sbjct: 68  WPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHD 127

Query: 135 YFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVS 194
           YF+  + ++   +++ AL      P+ +    + I+  +K   G  PG+ C  D  +  S
Sbjct: 128 YFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKD-SSHNS 187

Query: 195 YLVEVVACFAQDGSTLIDC 208
            L ++  C     S  I+C
Sbjct: 188 QLYQIYLCVDTSASKFINC 205

BLAST of MS026939 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 110.9 bits (276), Expect = 1.3e-24
Identity = 69/212 (32.55%), Postives = 98/212 (46.23%), Query Frame = 0

Query: 9   REVAISLALTIFSLMAFSDA----FDSFWFVQQWPPAVCSFQKSGSCP---GSGLRTFTI 68
           R + I   L + SL+  S      F+ F++V  WP A+C  QK G CP   G+    F I
Sbjct: 2   RGIIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQK-GCCPPTKGNTASDFII 61

Query: 69  HGLWPQ-QSGTSLTNCPGSP-FDITKISHLQSQLNTLWPN----VLRANNQQFWSHEWTK 128
           HGLWPQ  +GT    C  +  FDI+KIS L  Q+   W         +N    W HEW K
Sbjct: 62  HGLWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNK 121

Query: 129 HGTCSESTFNQAAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFP 188
           HGTC +S F+Q +YF+  +  ++   ++  L      PN    S   IK  +K   G  P
Sbjct: 122 HGTCVQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAP 181

Query: 189 GLRCRTDPQTKVSYLVEVVACFAQDGSTLIDC 208
           G+ C  D +     L ++  C        ++C
Sbjct: 182 GIECNEDVKGN-KQLFQIYICLDNYAKEFVEC 211

BLAST of MS026939 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 76.3 bits (186), Expect = 3.5e-14
Identity = 60/208 (28.85%), Postives = 86/208 (41.35%), Query Frame = 0

Query: 11  VAISLALTIFSLMAFSDAFDSFWFVQQWPPAVC-----SFQKSGSCPGSGLRT-FTIHGL 70
           +A + A  +  L      FD F    QWP   C        K+  C GS   T FTIHGL
Sbjct: 14  IAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGL 73

Query: 71  WPQ-QSGTSLTNCPGSPFDITKISHLQSQLNTLWPNV-------LRANNQQFWSHEWTKH 130
           WP    G+  + C  S F   +IS L   L   WP++              FW HEW KH
Sbjct: 74  WPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKH 133

Query: 131 GTCSESTF-NQAAYFKLAVDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKF- 190
           GTCS   F ++  YF   +++   +++   L  + AG       +  + G + A    F 
Sbjct: 134 GTCSSPVFHDEYNYFLTTLNLYLKHNVTDVL--YQAGYVASNSEKYPLGGIVTAIQNAFH 193

Query: 191 --PGLRCRTDPQTKVSYLVEVVACFAQD 201
             P + C+ D       + E+  CF +D
Sbjct: 194 ITPEVVCKRDA------IDEIRICFYKD 213