MS026778 (gene) Bitter gourd (TR) v1

Overview
NameMS026778
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionprotein ROOT PRIMORDIUM DEFECTIVE 1
Locationscaffold300: 138743 .. 139855 (+)
RNA-Seq ExpressionMS026778
SyntenyMS026778
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTCATCGCTATCCTTCCATTTTTGAACTTTTTTCAATCCCTTATCCACCCACACCACTCAATGCAACAAAGCTATATCCCCAACTTTGTGTTCGTCTAACCCCAGCAGCAGCATCTCTTGCTAGACAGGACTCGGATCTCAAATTGGCTATCTCTAACACCTTGGCTGAAAAGCTCCAAAAATTACTTATGCTTTCTTCACACCACAGGATTCTATTATCAAAGTTGGTTCACCTTGCTCCAGATCTAAGCATGCCCCCTAATTTTAGGTCCCGTCTCTGCAATGATTTTCCAGACAAATTCAGGACTGTTGATACCTCATATGGTCGGGCACTAGAGCTTGTCTCTTGGGACTCAGAGTTGGCAAAGCCTTTACCTTGCCCTCAAGTTCCTTCACGTGAGCTAATAGTCGACAGGCCATTAAAATTCAACCTGTTGAAACTAAGAAAGGGGCTGAATTTGAAAAGAGCCCACCAGGAATTTCTAATTAAGTTCAGGGATCTGCCAGATGTTTGCCCTTACAAAACTCCTGCAACTGAGTTGGCTAAGGAGTCTATTGAGTCGGAAAAACGAGCTTGTGCTGTGGTACGAGAGATATTGGGGATGATGGTCGAGAAGAGGACTTTAATAGACCACTTAACACATTTCAGAAAAGATTTTGAGCTACCCAATAAACTGAGAGGCATGATTGTGAGGCATCCAGAGTTATTTTATGTGAGTCTGAAAGGTCAGAGAGACTCTGTATTCCTTGTTGAGGGGTTCGATGATAAGGGTGTTCTAGTGGAAAAGGACGAGACTATGGTAATAAAAAATCAATGGATGAAGCTCGTAAGGGAAGGGAAAAGGATGAGACGGGAGAAGAGGAAAGCTCAAATTTATGACAGTAAATATGGAAATTATCATGAAGACAATAATCATGATTACGAGGTAGAAACTGACTATGATGATGATTATGATGATGGTTTTGAGAGTTTATTTGAGTTTGAGGATTCAGATTTTGAGAATGAAAGTGATGACCTGCTGAGCAATAGGTCGAATGGAGATTTTTGGACTACAATTAATGCAGATATTATTGATGATGCAGATGGAGGACATATTGAACCTTGGTGA

mRNA sequence

ATGATTCATCGCTATCCTTCCATTTTTGAACTTTTTTCAATCCCTTATCCACCCACACCACTCAATGCAACAAAGCTATATCCCCAACTTTGTGTTCGTCTAACCCCAGCAGCAGCATCTCTTGCTAGACAGGACTCGGATCTCAAATTGGCTATCTCTAACACCTTGGCTGAAAAGCTCCAAAAATTACTTATGCTTTCTTCACACCACAGGATTCTATTATCAAAGTTGGTTCACCTTGCTCCAGATCTAAGCATGCCCCCTAATTTTAGGTCCCGTCTCTGCAATGATTTTCCAGACAAATTCAGGACTGTTGATACCTCATATGGTCGGGCACTAGAGCTTGTCTCTTGGGACTCAGAGTTGGCAAAGCCTTTACCTTGCCCTCAAGTTCCTTCACGTGAGCTAATAGTCGACAGGCCATTAAAATTCAACCTGTTGAAACTAAGAAAGGGGCTGAATTTGAAAAGAGCCCACCAGGAATTTCTAATTAAGTTCAGGGATCTGCCAGATGTTTGCCCTTACAAAACTCCTGCAACTGAGTTGGCTAAGGAGTCTATTGAGTCGGAAAAACGAGCTTGTGCTGTGGTACGAGAGATATTGGGGATGATGGTCGAGAAGAGGACTTTAATAGACCACTTAACACATTTCAGAAAAGATTTTGAGCTACCCAATAAACTGAGAGGCATGATTGTGAGGCATCCAGAGTTATTTTATGTGAGTCTGAAAGGTCAGAGAGACTCTGTATTCCTTGTTGAGGGGTTCGATGATAAGGGTGTTCTAGTGGAAAAGGACGAGACTATGGTAATAAAAAATCAATGGATGAAGCTCGTAAGGGAAGGGAAAAGGATGAGACGGGAGAAGAGGAAAGCTCAAATTTATGACAGTAAATATGGAAATTATCATGAAGACAATAATCATGATTACGAGGTAGAAACTGACTATGATGATGATTATGATGATGGTTTTGAGAGTTTATTTGAGTTTGAGGATTCAGATTTTGAGAATGAAAGTGATGACCTGCTGAGCAATAGGTCGAATGGAGATTTTTGGACTACAATTAATGCAGATATTATTGATGATGCAGATGGAGGACATATTGAACCTTGGTGA

Coding sequence (CDS)

ATGATTCATCGCTATCCTTCCATTTTTGAACTTTTTTCAATCCCTTATCCACCCACACCACTCAATGCAACAAAGCTATATCCCCAACTTTGTGTTCGTCTAACCCCAGCAGCAGCATCTCTTGCTAGACAGGACTCGGATCTCAAATTGGCTATCTCTAACACCTTGGCTGAAAAGCTCCAAAAATTACTTATGCTTTCTTCACACCACAGGATTCTATTATCAAAGTTGGTTCACCTTGCTCCAGATCTAAGCATGCCCCCTAATTTTAGGTCCCGTCTCTGCAATGATTTTCCAGACAAATTCAGGACTGTTGATACCTCATATGGTCGGGCACTAGAGCTTGTCTCTTGGGACTCAGAGTTGGCAAAGCCTTTACCTTGCCCTCAAGTTCCTTCACGTGAGCTAATAGTCGACAGGCCATTAAAATTCAACCTGTTGAAACTAAGAAAGGGGCTGAATTTGAAAAGAGCCCACCAGGAATTTCTAATTAAGTTCAGGGATCTGCCAGATGTTTGCCCTTACAAAACTCCTGCAACTGAGTTGGCTAAGGAGTCTATTGAGTCGGAAAAACGAGCTTGTGCTGTGGTACGAGAGATATTGGGGATGATGGTCGAGAAGAGGACTTTAATAGACCACTTAACACATTTCAGAAAAGATTTTGAGCTACCCAATAAACTGAGAGGCATGATTGTGAGGCATCCAGAGTTATTTTATGTGAGTCTGAAAGGTCAGAGAGACTCTGTATTCCTTGTTGAGGGGTTCGATGATAAGGGTGTTCTAGTGGAAAAGGACGAGACTATGGTAATAAAAAATCAATGGATGAAGCTCGTAAGGGAAGGGAAAAGGATGAGACGGGAGAAGAGGAAAGCTCAAATTTATGACAGTAAATATGGAAATTATCATGAAGACAATAATCATGATTACGAGGTAGAAACTGACTATGATGATGATTATGATGATGGTTTTGAGAGTTTATTTGAGTTTGAGGATTCAGATTTTGAGAATGAAAGTGATGACCTGCTGAGCAATAGGTCGAATGGAGATTTTTGGACTACAATTAATGCAGATATTATTGATGATGCAGATGGAGGACATATTGAACCTTGGTGA

Protein sequence

MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQIYDSKYGNYHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSNRSNGDFWTTINADIIDDADGGHIEPW
Homology
BLAST of MS026778 vs. NCBI nr
Match: XP_022145187.1 (protein ROOT PRIMORDIUM DEFECTIVE 1 [Momordica charantia] >XP_022145188.1 protein ROOT PRIMORDIUM DEFECTIVE 1 [Momordica charantia])

HSP 1 Score: 729.9 bits (1883), Expect = 1.1e-206
Identity = 367/370 (99.19%), Postives = 369/370 (99.73%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL
Sbjct: 115 MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 174

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS
Sbjct: 175 QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 234

Query: 121 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 180
           ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT
Sbjct: 235 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 294

Query: 181 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 240
           ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV
Sbjct: 295 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 354

Query: 241 SLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQIYDSKYGN 300
           SLKGQRDSVFLVEGFDDKGVLV+KDETMVIKNQWMKLVREGKRMRREK KAQIYDSKYGN
Sbjct: 355 SLKGQRDSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGN 414

Query: 301 YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSNRSNGDFWTTINADIID 360
           YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLS+RSNGDFWTTINADIID
Sbjct: 415 YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINADIID 474

Query: 361 DADGGHIEPW 371
           DADGGHIEPW
Sbjct: 475 DADGGHIEPW 484

BLAST of MS026778 vs. NCBI nr
Match: XP_023527715.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 659.4 bits (1700), Expect = 1.8e-185
Identity = 331/371 (89.22%), Postives = 354/371 (95.42%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLA+QDS+LKL ISNTLAEKL
Sbjct: 115 MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLAKQDSNLKLVISNTLAEKL 174

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCND+P+KFRTVDTSYGRALELV WD 
Sbjct: 175 QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDP 234

Query: 121 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 180
           ELAKPLPC QV SRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPA+
Sbjct: 235 ELAKPLPCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAS 294

Query: 181 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 240
           ELAKES+ESEKRACAVVRE+LGMM+EKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYV
Sbjct: 295 ELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYV 354

Query: 241 SLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQIYDSKYGN 300
           SLKGQRDSVFLVEGF+DKG L+EKDET+ IKNQWMKL+ EGKRMRREKRKAQIYDS+YGN
Sbjct: 355 SLKGQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGN 414

Query: 301 YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSNRSNGDFWTTIN-ADII 360
            HE+ +HD+E+ETDYDDDY+DGFESLF++ED DFE+E  DL SNRSNGDFWTT N ADII
Sbjct: 415 DHENYSHDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADII 474

Query: 361 DDADGGHIEPW 371
           +DA+GGHIEPW
Sbjct: 475 NDANGGHIEPW 485

BLAST of MS026778 vs. NCBI nr
Match: XP_038904265.1 (protein WHAT'S THIS FACTOR 1, chloroplastic [Benincasa hispida] >XP_038904305.1 protein WHAT'S THIS FACTOR 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 656.4 bits (1692), Expect = 1.5e-184
Identity = 326/370 (88.11%), Postives = 352/370 (95.14%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           MIHRYPSIFELFSIPYPPTPLNATK+YPQLCVRLTPAAASLA+QDSDLKL ISNTLAEKL
Sbjct: 115 MIHRYPSIFELFSIPYPPTPLNATKVYPQLCVRLTPAAASLAKQDSDLKLVISNTLAEKL 174

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCND+P+KFRTVDTSYGRALELVSWD 
Sbjct: 175 QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVSWDP 234

Query: 121 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 180
           ELAKPLPC QVPSRELIVDRPLKFNLL+LRKGLNLKRAHQEFLIKFRDLPDVCPYKTPA+
Sbjct: 235 ELAKPLPCLQVPSRELIVDRPLKFNLLRLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAS 294

Query: 181 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 240
           ELAKES+ESEKRACAVVRE+LGMM+EKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYV
Sbjct: 295 ELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYV 354

Query: 241 SLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQIYDSKYGN 300
           SLKGQRDSVFLVEGFD+KGVLV+KDET+ IKNQWMKL+ EGKRMR+EK+KA+ YDSKYGN
Sbjct: 355 SLKGQRDSVFLVEGFDEKGVLVKKDETLAIKNQWMKLLMEGKRMRQEKKKARKYDSKYGN 414

Query: 301 YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSNRSNGDFWTTINADIID 360
            H+++NHD+E+ETD+DDDYDDGFESLF++ED DFE+E  DL SN  NGDFW T N DII+
Sbjct: 415 EHKNDNHDHEMETDFDDDYDDGFESLFQYEDLDFEDERSDLPSNWLNGDFWITNNVDIIN 474

Query: 361 DADGGHIEPW 371
           DADGG IEPW
Sbjct: 475 DADGGRIEPW 484

BLAST of MS026778 vs. NCBI nr
Match: XP_004152293.1 (protein WHAT'S THIS FACTOR 1, chloroplastic [Cucumis sativus] >XP_031739932.1 protein WHAT'S THIS FACTOR 1, chloroplastic [Cucumis sativus] >KGN53093.1 hypothetical protein Csa_015066 [Cucumis sativus])

HSP 1 Score: 654.1 bits (1686), Expect = 7.4e-184
Identity = 324/370 (87.57%), Postives = 349/370 (94.32%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAAS+A+QDSDLK+ ISN LAEKL
Sbjct: 115 MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASIAKQDSDLKMVISNKLAEKL 174

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           QKLLMLSSHHRILLSKLVHLAPDLS+PPNFRSRLCND+P+KFRTVDTSYGRALELVSWD 
Sbjct: 175 QKLLMLSSHHRILLSKLVHLAPDLSLPPNFRSRLCNDYPEKFRTVDTSYGRALELVSWDP 234

Query: 121 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 180
           ELAKPLPC QVPSRELIVDRPLKFNLL+LRKGLNLKR HQEFLIKFRDLPDVCPYK PA+
Sbjct: 235 ELAKPLPCIQVPSRELIVDRPLKFNLLRLRKGLNLKRTHQEFLIKFRDLPDVCPYKNPAS 294

Query: 181 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 240
           ELAKES+ESEKRACAVVRE+LGMMVEKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYV
Sbjct: 295 ELAKESLESEKRACAVVREVLGMMVEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYV 354

Query: 241 SLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQIYDSKYGN 300
           SLKGQRDSVFLVEGFDDKGVL+EKDET+ IKNQWM L++E KR+RREK+KAQIYDSKYGN
Sbjct: 355 SLKGQRDSVFLVEGFDDKGVLLEKDETLAIKNQWMTLLKEAKRLRREKKKAQIYDSKYGN 414

Query: 301 YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSNRSNGDFWTTINADIID 360
            HE+NNHD+E+E DYDDDYDDGFESLF++ED DFE+E+  + S  SNGDFWTT N DI +
Sbjct: 415 DHENNNHDHEMENDYDDDYDDGFESLFQYEDLDFEDENSGVPSIWSNGDFWTTNNVDISN 474

Query: 361 DADGGHIEPW 371
           DADGGHIEPW
Sbjct: 475 DADGGHIEPW 484

BLAST of MS026778 vs. NCBI nr
Match: XP_022955645.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita moschata] >XP_022955646.1 protein WHAT'S THIS FACTOR 1 [Cucurbita moschata] >KAG7018490.1 Protein ROOT PRIMORDIUM DEFECTIVE 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 652.9 bits (1683), Expect = 1.7e-183
Identity = 327/371 (88.14%), Postives = 352/371 (94.88%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTP AASLA+QDS+LKL ISNTLAEKL
Sbjct: 115 MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKL 174

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCND+P+KFRTVDTSYGRALELV WD 
Sbjct: 175 QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDP 234

Query: 121 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 180
           ELAKPLPC QV SRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPA+
Sbjct: 235 ELAKPLPCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAS 294

Query: 181 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 240
           ELAKES+ESEKRACAVVRE+LGMM+EKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYV
Sbjct: 295 ELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYV 354

Query: 241 SLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQIYDSKYGN 300
           SLKGQRDSVFLVEGF+DKG L+EKDET+ IKNQWMKL+ EGKRMRREKRKAQIYDS+YGN
Sbjct: 355 SLKGQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGN 414

Query: 301 YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSNRSNGDFWTTIN-ADII 360
            HE+++HD+E+ETDYDDDY+DGFESLF++ED DFE+E  DL SNRSNGDFWTT N ADII
Sbjct: 415 DHENHSHDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADII 474

Query: 361 DDADGGHIEPW 371
           +  +GGHI+PW
Sbjct: 475 NGENGGHIKPW 485

BLAST of MS026778 vs. ExPASy Swiss-Prot
Match: B6TTV8 (Protein WHAT'S THIS FACTOR 1, chloroplastic OS=Zea mays OX=4577 GN=WTF1 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.6e-51
Identity = 142/366 (38.80%), Postives = 193/366 (52.73%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           ++ R+P +F++              +Y  L  RLTPAA  L   +  L+         KL
Sbjct: 114 LLRRFPGVFDVVE----------EGVY-SLRFRLTPAAERLYLDELRLRNESEGLAVAKL 173

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           +KLLM+S   RIL+ K+ HL  DL +PP FR  +C  +P  FR V    G ALEL  WD 
Sbjct: 174 RKLLMMSQEKRILIEKVAHLKHDLGLPPEFRDTVCLRYPQYFRVVRMDRGPALELTHWDP 233

Query: 121 ELA--------KPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDV 180
           ELA        +     +   R LI+DRPLKFN ++L KGL L R     + +F+++P +
Sbjct: 234 ELAVSAAELAEEESRAREAEERNLIIDRPLKFNRVRLPKGLKLTRGEARRIARFKEMPYI 293

Query: 181 CPYKTPATELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIV 240
            PY    + L   S E EK AC VV EIL + VEKRTL+DHLTHFR++F     LRGMI+
Sbjct: 294 SPY-ADFSHLRSGSDEKEKHACGVVHEILSLTVEKRTLVDHLTHFREEFRFSQSLRGMII 353

Query: 241 RHPELFYVSLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQ 300
           RHP++FYVS KG RDSVFL E + D   LVEK++ +++K +   LV     + R  R+A 
Sbjct: 354 RHPDMFYVSFKGDRDSVFLREAYKD-SQLVEKNQLVLLKEKMRALV----AVPRFPRRAA 413

Query: 301 IYDSKYGNYHEDNNHDYEVETDY-DDDYDDGFESLFEFEDSDFENESDDLLSNRSNGDFW 358
           +     G   E  N   +      D++YDD  E L + ED   E       ++   GD W
Sbjct: 414 V---GTGEEAEGMNGSLQSRDQVSDEEYDDEDEGLSDMEDLISELSGGKSDADYEWGDGW 459

BLAST of MS026778 vs. ExPASy Swiss-Prot
Match: Q65XL5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0571100 PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.0e-50
Identity = 141/384 (36.72%), Postives = 199/384 (51.82%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           ++ R+P +FE+              +Y  L  RLTPAA  L   +  LK         KL
Sbjct: 120 LLKRFPGVFEVVE----------EGVY-SLRFRLTPAAERLYLDELHLKNESEGLAVTKL 179

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           +KLLM+S   RIL+ K+ HL  DL +PP FR  +C  +P  FR V    G  LEL  WD 
Sbjct: 180 RKLLMMSQDKRILIEKIAHLKNDLGLPPEFRDTICLRYPQYFRVVQMDRGPGLELTHWDP 239

Query: 121 ELA--------KPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDV 180
           ELA        +     +   R LI+DRPLKFN +KL +GL L R     + +F+++P +
Sbjct: 240 ELAVSAAEVAEEENRAREEQERNLIIDRPLKFNRVKLPQGLKLSRGEARRVAQFKEMPYI 299

Query: 181 CPYKTPATELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIV 240
            PY +  + L   S E EK AC VV EIL + +EKRTL+DHLTHFR++F     LRGM++
Sbjct: 300 SPY-SDFSHLRSGSAEKEKHACGVVHEILSLTLEKRTLVDHLTHFREEFRFSQSLRGMLI 359

Query: 241 RHPELFYVSLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQ 300
           RHP++FYVSLKG RDSVFL E + +   LVEK + +++K +   LV     + R  R+  
Sbjct: 360 RHPDMFYVSLKGDRDSVFLREAYKN-SQLVEKSKLVLLKEKMRALV----AVPRFPRRGV 419

Query: 301 IYDSKYGNYHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSNRSN----- 360
              S+  +            +D +DD D+G         SD E+   ++   +S+     
Sbjct: 420 PATSEEADRTNGAAQMLSEGSDVEDDEDEGL--------SDMEDLISEISGGKSDTDYHW 478

Query: 361 GDFWTTINADI---IDDADGGHIE 369
           GD W   N D     +D DG  ++
Sbjct: 480 GDGWVGENDDSPPDFEDDDGSSLK 478

BLAST of MS026778 vs. ExPASy Swiss-Prot
Match: A0MFS5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01037 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.5e-49
Identity = 136/370 (36.76%), Postives = 195/370 (52.70%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           ++ +YP +FE+             +    L  ++T  A  L   +  ++  + + L  KL
Sbjct: 135 LLRKYPGVFEI-----------VEEGAYSLRFKMTSEAERLYLDEMRIRNELEDVLVVKL 194

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           +KL+M+S   RILL K+ HL  DL +P  FR  +C  +P  FR V T  G ALEL  WD 
Sbjct: 195 RKLVMMSIDKRILLEKISHLKTDLGLPLEFRDTICQRYPQYFRVVPTPRGPALELTHWDP 254

Query: 121 ELAKPLP--------CPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDV 180
           ELA              +   R LI+DRP KFN +KL +GLNL ++    + +FRD+  +
Sbjct: 255 ELAVSAAELSEDDNRTRESEERNLIIDRPPKFNRVKLPRGLNLSKSETRKISQFRDMQYI 314

Query: 181 CPYKTPATELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIV 240
            PYK   + L   ++E EK AC V+ E+L +  EKRTL+DHLTHFR++F    +LRGM++
Sbjct: 315 SPYK-DFSHLRSGTLEKEKHACGVIHELLSLTTEKRTLVDHLTHFREEFRFSQQLRGMLI 374

Query: 241 RHPELFYVSLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLV-------REGKRMR 300
           RHP+LFYVSLKG+RDSVFL E + +   L++KD   ++K +   LV       R G R  
Sbjct: 375 RHPDLFYVSLKGERDSVFLREAYRN-SELIDKDPLTLVKEKMRALVSVPRFPRRGGPRKD 434

Query: 301 REKRKAQIYDSKYGNYHEDNNHDYEVETDY-----------DDD----YDDGFESLFEFE 341
            E R+ +I  S      E+   D E  +D            DDD     D+G E +    
Sbjct: 435 EEGREVEIDGSDADGEEEEEESDAEEWSDVDGYLEGEDGGNDDDGDWTDDEGEEDVPPNF 491

BLAST of MS026778 vs. ExPASy Swiss-Prot
Match: Q9ZUZ6 (Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=WTF9 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.8e-15
Identity = 76/293 (25.94%), Postives = 138/293 (47.10%), Query Frame = 0

Query: 2   IHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQ 61
           + ++PSIFE F  P    P            RLTP A  L RQ+  +    ++ L ++L+
Sbjct: 89  LRKFPSIFEEFVGPEYNLP----------WFRLTPEATELDRQERVVYQTSADDLRDRLK 148

Query: 62  KLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTS---YGRALELVSW 121
           KL+++S  + + LS +  +   L +P ++      +    FR VD      G A++    
Sbjct: 149 KLILMSKDNVLPLSIVQGMKWYLGLPDDYLQFPDMNLDSSFRFVDMEDGVKGLAVDYNGG 208

Query: 122 DSELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTP 181
           D  L+          R  +    ++F L    KG  L+   +++L++F+ LP V PY   
Sbjct: 209 DKVLSVLQKNAMKKRRGEVSLEEIEFPLFP-SKGCRLRVKIEDWLMEFQKLPYVSPYDDY 268

Query: 182 ATELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELF 241
           +  L   S  +EKR    + E+L + VE       L   +K F LP K+     RHP++F
Sbjct: 269 SC-LDPSSDIAEKRVVGFLHELLCLFVEHSAERKKLLCLKKHFGLPQKVHKAFERHPQIF 328

Query: 242 YVSLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKA 292
           Y+S+K +  +  L E + DK   VE    + ++ ++++L++  + + + +R +
Sbjct: 329 YLSMKNKTCTAILREPYRDK-ASVETHPVLGVRKKYIQLMKNSELILKSRRNS 368

BLAST of MS026778 vs. ExPASy Swiss-Prot
Match: Q689D6 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=RPD1 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 8.4e-13
Identity = 80/324 (24.69%), Postives = 153/324 (47.22%), Query Frame = 0

Query: 4   RYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKL 63
           ++P +FE++  P          +   L  RLT  A    R + +  L        +L+KL
Sbjct: 107 KFPHVFEIYEHP----------VQRILYCRLTRKALDQIRHEHEAVLDQIPDAVTRLRKL 166

Query: 64  LMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVD--TSYGRALELVSWDSE 123
           +M+S+  RI L  +     +  +P +F   +    P  FR +D   +  + +E+V  D  
Sbjct: 167 VMMSNTGRIRLEHVRIARTEFGLPEDFEYSVILKHPQFFRLIDGEETRDKYIEIVEKDPN 226

Query: 124 LAKPLPCPQVPSREL------IVDRPLKFN-LLKLRKGLNLKRAHQEFLIKFRDLPDVCP 183
           L+    C     RE+      I    ++F+ ++    G  + +  +  + K++ LP   P
Sbjct: 227 LS---ICAIERVREIEYRTKGIDAEDVRFSFVVNFPPGFKIGKYFRIAVWKWQRLPYWSP 286

Query: 184 YK-TPATELAKESIES--EKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMI 243
           Y+     +L     ++  EKR+ A + E+L + VEK+  ++ + HFR    LP KL+  +
Sbjct: 287 YEDISGYDLRSMEAQNRLEKRSVACIHELLSLTVEKKITLERIAHFRNVMNLPKKLKEFL 346

Query: 244 VRHPELFYVSLK---GQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREK 303
           ++H  +FY+S +   G+  +VFL EG+  +G LVE ++  + + +  +LV          
Sbjct: 347 LQHQGIFYISTRGNYGKLHTVFLREGY-KRGELVEPNDVYLARRRLAELV------LMSP 406

Query: 304 RKAQIYDSKYGNYHEDNNHDYEVE 313
           RKA++ D++   Y +  + + +VE
Sbjct: 407 RKAKV-DAELVRYRDGLDDEDDVE 409

BLAST of MS026778 vs. ExPASy TrEMBL
Match: A0A6J1CVW2 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Momordica charantia OX=3673 GN=LOC111014693 PE=4 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 5.1e-207
Identity = 367/370 (99.19%), Postives = 369/370 (99.73%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL
Sbjct: 115 MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 174

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS
Sbjct: 175 QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 234

Query: 121 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 180
           ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT
Sbjct: 235 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 294

Query: 181 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 240
           ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV
Sbjct: 295 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 354

Query: 241 SLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQIYDSKYGN 300
           SLKGQRDSVFLVEGFDDKGVLV+KDETMVIKNQWMKLVREGKRMRREK KAQIYDSKYGN
Sbjct: 355 SLKGQRDSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGN 414

Query: 301 YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSNRSNGDFWTTINADIID 360
           YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLS+RSNGDFWTTINADIID
Sbjct: 415 YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINADIID 474

Query: 361 DADGGHIEPW 371
           DADGGHIEPW
Sbjct: 475 DADGGHIEPW 484

BLAST of MS026778 vs. ExPASy TrEMBL
Match: A0A0A0KU86 (PORR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015830 PE=4 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.6e-184
Identity = 324/370 (87.57%), Postives = 349/370 (94.32%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAAS+A+QDSDLK+ ISN LAEKL
Sbjct: 115 MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASIAKQDSDLKMVISNKLAEKL 174

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           QKLLMLSSHHRILLSKLVHLAPDLS+PPNFRSRLCND+P+KFRTVDTSYGRALELVSWD 
Sbjct: 175 QKLLMLSSHHRILLSKLVHLAPDLSLPPNFRSRLCNDYPEKFRTVDTSYGRALELVSWDP 234

Query: 121 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 180
           ELAKPLPC QVPSRELIVDRPLKFNLL+LRKGLNLKR HQEFLIKFRDLPDVCPYK PA+
Sbjct: 235 ELAKPLPCIQVPSRELIVDRPLKFNLLRLRKGLNLKRTHQEFLIKFRDLPDVCPYKNPAS 294

Query: 181 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 240
           ELAKES+ESEKRACAVVRE+LGMMVEKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYV
Sbjct: 295 ELAKESLESEKRACAVVREVLGMMVEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYV 354

Query: 241 SLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQIYDSKYGN 300
           SLKGQRDSVFLVEGFDDKGVL+EKDET+ IKNQWM L++E KR+RREK+KAQIYDSKYGN
Sbjct: 355 SLKGQRDSVFLVEGFDDKGVLLEKDETLAIKNQWMTLLKEAKRLRREKKKAQIYDSKYGN 414

Query: 301 YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSNRSNGDFWTTINADIID 360
            HE+NNHD+E+E DYDDDYDDGFESLF++ED DFE+E+  + S  SNGDFWTT N DI +
Sbjct: 415 DHENNNHDHEMENDYDDDYDDGFESLFQYEDLDFEDENSGVPSIWSNGDFWTTNNVDISN 474

Query: 361 DADGGHIEPW 371
           DADGGHIEPW
Sbjct: 475 DADGGHIEPW 484

BLAST of MS026778 vs. ExPASy TrEMBL
Match: A0A6J1GVP1 (protein WHAT'S THIS FACTOR 1 OS=Cucurbita moschata OX=3662 GN=LOC111457587 PE=4 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 8.0e-184
Identity = 327/371 (88.14%), Postives = 352/371 (94.88%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTP AASLA+QDS+LKL ISNTLAEKL
Sbjct: 115 MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKL 174

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCND+P+KFRTVDTSYGRALELV WD 
Sbjct: 175 QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDP 234

Query: 121 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 180
           ELAKPLPC QV SRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPA+
Sbjct: 235 ELAKPLPCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAS 294

Query: 181 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 240
           ELAKES+ESEKRACAVVRE+LGMM+EKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYV
Sbjct: 295 ELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYV 354

Query: 241 SLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQIYDSKYGN 300
           SLKGQRDSVFLVEGF+DKG L+EKDET+ IKNQWMKL+ EGKRMRREKRKAQIYDS+YGN
Sbjct: 355 SLKGQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGN 414

Query: 301 YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSNRSNGDFWTTIN-ADII 360
            HE+++HD+E+ETDYDDDY+DGFESLF++ED DFE+E  DL SNRSNGDFWTT N ADII
Sbjct: 415 DHENHSHDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADII 474

Query: 361 DDADGGHIEPW 371
           +  +GGHI+PW
Sbjct: 475 NGENGGHIKPW 485

BLAST of MS026778 vs. ExPASy TrEMBL
Match: A0A6J1IUA5 (protein WHAT'S THIS FACTOR 1 OS=Cucurbita maxima OX=3661 GN=LOC111479376 PE=4 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 3.0e-183
Identity = 327/371 (88.14%), Postives = 351/371 (94.61%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLA+QDS+LKL ISNTLAEKL
Sbjct: 115 MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLAKQDSNLKLVISNTLAEKL 174

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCND+P+KFRTVDTSYGRALELV WD 
Sbjct: 175 QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDP 234

Query: 121 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 180
           ELAK LPC QV SRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPA+
Sbjct: 235 ELAKTLPCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAS 294

Query: 181 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 240
           ELAKES+ESEKRACAVVRE+LGMM+EKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYV
Sbjct: 295 ELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYV 354

Query: 241 SLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQIYDSKYGN 300
           SLKGQRDSVFLVEGF+DKG L+EKDET+ IKNQWMKL+ EGKRMRREKRKAQIYDS+YGN
Sbjct: 355 SLKGQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGN 414

Query: 301 YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSNRSNGDFWTTIN-ADII 360
            HE+++HD+E+ETDYDDDY+DGFESLF++ED DFE+E  DL SNRSNGDFWTT N ADI 
Sbjct: 415 DHENHSHDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIT 474

Query: 361 DDADGGHIEPW 371
           +  +GGHIEPW
Sbjct: 475 NGENGGHIEPW 485

BLAST of MS026778 vs. ExPASy TrEMBL
Match: A0A5A7TP08 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003590 PE=4 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 3.0e-183
Identity = 322/370 (87.03%), Postives = 348/370 (94.05%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLT AAAS+A+QDSDLKL ISNTLAEKL
Sbjct: 114 MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTAAAASIAKQDSDLKLVISNTLAEKL 173

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCND+P+KFRTVDTSYGRALELVSWD 
Sbjct: 174 QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVSWDP 233

Query: 121 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 180
           ELAKPLPC QVPSRELIVDRPLKFNLL+LRKGLNLKR HQEFLIKFRDLPD+CPYKTPA+
Sbjct: 234 ELAKPLPCIQVPSRELIVDRPLKFNLLRLRKGLNLKRTHQEFLIKFRDLPDICPYKTPAS 293

Query: 181 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 240
           ELAKES+ESEKRACAVVRE+LGMM+EKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYV
Sbjct: 294 ELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYV 353

Query: 241 SLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQIYDSKYGN 300
           SLKGQRDSVFLVEGFDDKG L+EKDET+ IKN+WM L++EGKRMRREK+KAQIYDSKYGN
Sbjct: 354 SLKGQRDSVFLVEGFDDKGALLEKDETLAIKNRWMALLKEGKRMRREKKKAQIYDSKYGN 413

Query: 301 YHEDNNHDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSNRSNGDFWTTINADIID 360
            HE+NNHD+E+E DYDD+YDDGFESLF++ED DFE+E+  + S  SNGDFWTT N DI +
Sbjct: 414 DHENNNHDHEMENDYDDNYDDGFESLFQYEDLDFEDENSGVPSIWSNGDFWTTNNVDISN 473

Query: 361 DADGGHIEPW 371
           D DG HIEPW
Sbjct: 474 DTDGSHIEPW 483

BLAST of MS026778 vs. TAIR 10
Match: AT5G62990.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 421.4 bits (1082), Expect = 7.5e-118
Identity = 230/381 (60.37%), Postives = 287/381 (75.33%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           MI RYP+IFELF+ P P  P+NATK   QLCVRLT AA+SLA Q+ +LK  IS+ LA KL
Sbjct: 116 MIRRYPTIFELFTTPTPHLPMNATKPLSQLCVRLTSAASSLAMQELNLKSEISDKLATKL 175

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           QKLLMLSSH R+LLSKLVH+APD   PPNFRSRLCND+PDKF+TVDTSYGRALELVS D 
Sbjct: 176 QKLLMLSSHRRLLLSKLVHIAPDFGFPPNFRSRLCNDYPDKFKTVDTSYGRALELVSSDP 235

Query: 121 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 180
           ELA  +P P+V  R LIVDRPLKF  L LR+GLNLKR HQ FLIKFR+ PDVCPYK  + 
Sbjct: 236 ELANQMPSPEV-DRGLIVDRPLKFKRLNLRRGLNLKRRHQGFLIKFRESPDVCPYKMSSD 295

Query: 181 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 240
            LA ESIE+EKRACAVVRE+LG+ VEKRTLIDHLTHFRK+F LPNKLR +IVRHPELFYV
Sbjct: 296 YLASESIEAEKRACAVVREVLGLTVEKRTLIDHLTHFRKEFSLPNKLRDLIVRHPELFYV 355

Query: 241 SLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRK--AQIYDSKY 300
           S+KG RDSVFLVE ++D G L++KDE +VI+ + + L++EGKR+RRE+R+  A + D   
Sbjct: 356 SIKGMRDSVFLVEAYNDNGDLLDKDERLVIRERLIDLIQEGKRIRRERRRKGATLGDKSV 415

Query: 301 GNYHEDNNHDYEVETDYDDDYDDGFESLFEFED------SDFENESDDLLSNRSNGDFWT 360
               +D+    + ++D DD+Y+DGFE+LF+ ED       D E++ D+   N  + ++W+
Sbjct: 416 EEKRKDDETIDDYDSDLDDEYEDGFENLFDSEDLGVEYHFDEEDDDDEAGVNGESVEYWS 475

Query: 361 ---TINADIIDDADGGHIEPW 371
              + +    D+A+   +E W
Sbjct: 476 RKLSSSGSSSDEANSA-VESW 494

BLAST of MS026778 vs. TAIR 10
Match: AT4G01037.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 198.4 bits (503), Expect = 1.0e-50
Identity = 136/370 (36.76%), Postives = 195/370 (52.70%), Query Frame = 0

Query: 1   MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 60
           ++ +YP +FE+             +    L  ++T  A  L   +  ++  + + L  KL
Sbjct: 135 LLRKYPGVFEI-----------VEEGAYSLRFKMTSEAERLYLDEMRIRNELEDVLVVKL 194

Query: 61  QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDS 120
           +KL+M+S   RILL K+ HL  DL +P  FR  +C  +P  FR V T  G ALEL  WD 
Sbjct: 195 RKLVMMSIDKRILLEKISHLKTDLGLPLEFRDTICQRYPQYFRVVPTPRGPALELTHWDP 254

Query: 121 ELAKPLP--------CPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDV 180
           ELA              +   R LI+DRP KFN +KL +GLNL ++    + +FRD+  +
Sbjct: 255 ELAVSAAELSEDDNRTRESEERNLIIDRPPKFNRVKLPRGLNLSKSETRKISQFRDMQYI 314

Query: 181 CPYKTPATELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIV 240
            PYK   + L   ++E EK AC V+ E+L +  EKRTL+DHLTHFR++F    +LRGM++
Sbjct: 315 SPYK-DFSHLRSGTLEKEKHACGVIHELLSLTTEKRTLVDHLTHFREEFRFSQQLRGMLI 374

Query: 241 RHPELFYVSLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLV-------REGKRMR 300
           RHP+LFYVSLKG+RDSVFL E + +   L++KD   ++K +   LV       R G R  
Sbjct: 375 RHPDLFYVSLKGERDSVFLREAYRN-SELIDKDPLTLVKEKMRALVSVPRFPRRGGPRKD 434

Query: 301 REKRKAQIYDSKYGNYHEDNNHDYEVETDY-----------DDD----YDDGFESLFEFE 341
            E R+ +I  S      E+   D E  +D            DDD     D+G E +    
Sbjct: 435 EEGREVEIDGSDADGEEEEEESDAEEWSDVDGYLEGEDGGNDDDGDWTDDEGEEDVPPNF 491

BLAST of MS026778 vs. TAIR 10
Match: AT3G63090.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 103.6 bits (257), Expect = 3.5e-22
Identity = 85/290 (29.31%), Postives = 142/290 (48.97%), Query Frame = 0

Query: 6   PSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLM 65
           PS+FE++     P      K  P   +R TP   +   ++  +       L  KL +LLM
Sbjct: 98  PSLFEIYYDRIKP------KSDPVQFLRPTPRLRAFLDEEQRIYSENEPLLVSKLCQLLM 157

Query: 66  LSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFR-TVDTSYGRA-LELVSWDSELA 125
           ++    I   KLVH+  D   P +F  +L   +P+ FR T     G++ LELVSW+ + A
Sbjct: 158 MAKDKVISADKLVHVKRDFGFPNDFLMKLVQKYPNYFRLTGLPEEGKSFLELVSWNPDFA 217

Query: 126 KPLPCPQVPSRELIVDRPLKFNL-LKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATEL 185
           K     +     L     ++ N  +KL  G  L++  +E+   + +   + PY+   + L
Sbjct: 218 KSKIELRAEDETLKTGVRIRPNYNVKLPSGFFLRKEMREWTRDWLEQDYISPYE-DVSHL 277

Query: 186 AKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSL 245
            + S E EKR   VV E+L + + KR  +  L  F  +F   N    +  RH  +FY+SL
Sbjct: 278 DQASKEMEKRTVGVVHELLSLSLLKRVPVPTLGKFCDEFRFSNAFSSVFTRHSGIFYLSL 337

Query: 246 KGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLVREGKRMRREKRKAQ 293
           KG   +  L E + D   LV++D  + IK+++++L+ EG + R+++ K Q
Sbjct: 338 KGGIKTAVLREAYKD-DELVDRDPLLAIKDKFLRLLEEGWQERKDRLKLQ 379

BLAST of MS026778 vs. TAIR 10
Match: AT5G45790.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 103.6 bits (257), Expect = 3.5e-22
Identity = 90/324 (27.78%), Postives = 156/324 (48.15%), Query Frame = 0

Query: 2   IHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQ 61
           I +YP  FE+F+ P+             LC ++T     L  ++ ++         ++++
Sbjct: 40  IGKYPHAFEIFTHPFS----------KNLCCKITEKFKVLIDEEENVVRECEVDAVKRVK 99

Query: 62  KLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSE 121
           KLL+LS H  + +  L  +  +L +P +FR  +   +  +FR VD      LELV  D E
Sbjct: 100 KLLLLSKHGVLRVHALRLIRKELGLPEDFRDSILAKYSSEFRLVDL---ETLELVDRDDE 159

Query: 122 ---LAKPLPCPQVPSRELIVDRPLKFNL---LKLRKGLNLKRAHQEFLIKFRDLPDVCPY 181
              +AK     +V  RE  + +  + N    + L  G  +++  +E L  ++ +P V PY
Sbjct: 160 SLCVAKVEEWREVEYREKWLSK-FETNYAFPIHLPTGFKIEKGFREELKNWQRVPYVKPY 219

Query: 182 KTPATELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHP 241
                E+++     EKR  AV+ E+L + VEK   ++ L HFRKD  +   +R +I++HP
Sbjct: 220 --DRKEISRGLERFEKRVVAVIHELLSLTVEKMVEVERLAHFRKDLGIEVNVREVILKHP 279

Query: 242 ELFYVSLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLV---REGKRMRREKRKAQ 301
            +FYVS KG   ++FL E +  KG L+E +    ++ + + LV       RM  ++    
Sbjct: 280 GIFYVSTKGSSQTLFLREAY-SKGCLIEPNPIYNVRRKMLDLVLLKTRYSRMLLQREDET 339

Query: 302 IYDSKYGNYHEDNNHDYEVETDYD 317
             + K  +    +N D+E   D D
Sbjct: 340 HREEKSRDVLISSNEDWEGGRDGD 346

BLAST of MS026778 vs. TAIR 10
Match: AT5G45790.2 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 103.6 bits (257), Expect = 3.5e-22
Identity = 90/324 (27.78%), Postives = 156/324 (48.15%), Query Frame = 0

Query: 2   IHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQ 61
           I +YP  FE+F+ P+             LC ++T     L  ++ ++         ++++
Sbjct: 125 IGKYPHAFEIFTHPFS----------KNLCCKITEKFKVLIDEEENVVRECEVDAVKRVK 184

Query: 62  KLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSE 121
           KLL+LS H  + +  L  +  +L +P +FR  +   +  +FR VD      LELV  D E
Sbjct: 185 KLLLLSKHGVLRVHALRLIRKELGLPEDFRDSILAKYSSEFRLVDL---ETLELVDRDDE 244

Query: 122 ---LAKPLPCPQVPSRELIVDRPLKFNL---LKLRKGLNLKRAHQEFLIKFRDLPDVCPY 181
              +AK     +V  RE  + +  + N    + L  G  +++  +E L  ++ +P V PY
Sbjct: 245 SLCVAKVEEWREVEYREKWLSK-FETNYAFPIHLPTGFKIEKGFREELKNWQRVPYVKPY 304

Query: 182 KTPATELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHP 241
                E+++     EKR  AV+ E+L + VEK   ++ L HFRKD  +   +R +I++HP
Sbjct: 305 --DRKEISRGLERFEKRVVAVIHELLSLTVEKMVEVERLAHFRKDLGIEVNVREVILKHP 364

Query: 242 ELFYVSLKGQRDSVFLVEGFDDKGVLVEKDETMVIKNQWMKLV---REGKRMRREKRKAQ 301
            +FYVS KG   ++FL E +  KG L+E +    ++ + + LV       RM  ++    
Sbjct: 365 GIFYVSTKGSSQTLFLREAY-SKGCLIEPNPIYNVRRKMLDLVLLKTRYSRMLLQREDET 424

Query: 302 IYDSKYGNYHEDNNHDYEVETDYD 317
             + K  +    +N D+E   D D
Sbjct: 425 HREEKSRDVLISSNEDWEGGRDGD 431

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145187.11.1e-20699.19protein ROOT PRIMORDIUM DEFECTIVE 1 [Momordica charantia] >XP_022145188.1 protei... [more]
XP_023527715.11.8e-18589.22protein WHAT'S THIS FACTOR 1 [Cucurbita pepo subsp. pepo][more]
XP_038904265.11.5e-18488.11protein WHAT'S THIS FACTOR 1, chloroplastic [Benincasa hispida] >XP_038904305.1 ... [more]
XP_004152293.17.4e-18487.57protein WHAT'S THIS FACTOR 1, chloroplastic [Cucumis sativus] >XP_031739932.1 pr... [more]
XP_022955645.11.7e-18388.14protein WHAT'S THIS FACTOR 1 [Cucurbita moschata] >XP_022955646.1 protein WHAT'S... [more]
Match NameE-valueIdentityDescription
B6TTV81.6e-5138.80Protein WHAT'S THIS FACTOR 1, chloroplastic OS=Zea mays OX=4577 GN=WTF1 PE=1 SV=... [more]
Q65XL51.0e-5036.72Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Oryza sativa subsp. japon... [more]
A0MFS51.5e-4936.76Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q9ZUZ66.8e-1525.94Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=W... [more]
Q689D68.4e-1324.69Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=RPD1 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1CVW25.1e-20799.19protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Momordica charantia OX=3673 GN=LOC1110146... [more]
A0A0A0KU863.6e-18487.57PORR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015830 PE=4 S... [more]
A0A6J1GVP18.0e-18488.14protein WHAT'S THIS FACTOR 1 OS=Cucurbita moschata OX=3662 GN=LOC111457587 PE=4 ... [more]
A0A6J1IUA53.0e-18388.14protein WHAT'S THIS FACTOR 1 OS=Cucurbita maxima OX=3661 GN=LOC111479376 PE=4 SV... [more]
A0A5A7TP083.0e-18387.03Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
Match NameE-valueIdentityDescription
AT5G62990.17.5e-11860.37Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT4G01037.11.0e-5036.76Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT3G63090.13.5e-2229.31Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT5G45790.13.5e-2227.78Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT5G45790.23.5e-2227.78Ubiquitin carboxyl-terminal hydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021099Plant organelle RNA recognition domainPFAMPF11955PORRcoord: 1..283
e-value: 2.6E-81
score: 273.1
IPR045040PORR familyPANTHERPTHR31476PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTICcoord: 1..334
NoneNo IPR availablePANTHERPTHR31476:SF2UBIQUITIN CARBOXYL-TERMINAL HYDROLASE FAMILY PROTEINcoord: 1..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS026778.1MS026778.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding