MS026497 (gene) Bitter gourd (TR) v1

Overview


Name	MS026497
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	glutathione S-transferase U8-like
Location	scaffold607: 1382569 .. 1383115 (-)
RNA-Seq Expression	MS026497
Synteny	MS026497

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGAAGAACTGCAAGTGTTTGGTTTCTGGACAAGTCCTTTTAGTCGCAGAGTGGAGCTGGCTCTCAAACTCAAAGCCTTGGAATATGAATATGTTGAAGAAGATATATGGAACAAGAGCGATTTGCTTGTAAAATACAACCCAATTTACAAGAAGGTGCCTGTTCTCCTCCACCATGGAAAGCCCATCGCAGAGTCTCTTGTCATCCTCGAATACATTGAAGAAAATTGGAAGGCCAACCCCATCTTCCCCCACCATCCCCATCAAAGAGCTCTCGCCAGGTTCTGGGCTAAGTATATTGATGACAAGGTAACACCCTACCGTCCAATTTAATCTATACTTATAAGTAGAGTAAATTTAATCTTTTATCAATACTTGTAACAGATTGTGCCTGCTAAAAGTTGCTNAGTTGCTGACAAGCCACAAGTTTCCAAACATAACCAAATGGAGTGAAGAGCTGTTGAACCACAGCGTGGTGAAGGAGACTTTGCCTCCAAGAGCAGAGCTTGTTGCCTATTTGCAAGCTGTTTTTGGAGCCAACTAG

mRNA sequence

ATGGCGGAAGAACTGCAAGTGTTTGGTTTCTGGACAAGTCCTTTTAGTCGCAGAGTGGAGCTGGCTCTCAAACTCAAAGCCTTGGAATATGAATATGTTGAAGAAGATATATGGAACAAGAGCGATTTGCTTGTAAAATACAACCCAATTTACAAGAAGGTGCCTGTTCTCCTCCACCATGGAAAGCCCATCGCAGAGTCTCTTGTCATCCTCGAATACATTGAAGAAAATTGGAAGGCCAACCCCATCTTCCCCCACCATCCCCATCAAAGAGCTCTCGCCAGATTGTGCCTGCTAAAAGTTGCTNAGTTGCTGACAAGCCACAAGTTTCCAAACATAACCAAATGGAGTGAAGAGCTGTTGAACCACAGCGTGGTGAAGGAGACTTTGCCTCCAAGAGCAGAGCTTGTTGCCTATTTGCAAGCTGTTTTTGGAGCCAACTAG

Coding sequence (CDS)

ATGGCGGAAGAACTGCAAGTGTTTGGTTTCTGGACAAGTCCTTTTAGTCGCAGAGTGGAGCTGGCTCTCAAACTCAAAGCCTTGGAATATGAATATGTTGAAGAAGATATATGGAACAAGAGCGATTTGCTTGTAAAATACAACCCAATTTACAAGAAGGTGCCTGTTCTCCTCCACCATGGAAAGCCCATCGCAGAGTCTCTTGTCATCCTCGAATACATTGAAGAAAATTGGAAGGCCAACCCCATCTTCCCCCACCATCCCCATCAAAGAGCTCTCGCCAGATTGTGCCTGCTAAAAGTTGCTNAGTTGCTGACAAGCCACAAGTTTCCAAACATAACCAAATGGAGTGAAGAGCTGTTGAACCACAGCGTGGTGAAGGAGACTTTGCCTCCAAGAGCAGAGCTTGTTGCCTATTTGCAAGCTGTTTTTGGAGCCAACTAG

Protein sequence

MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHHGKPIAESLVILEYIEENWKANPIFPHHPHQRALARLCLLKVAXLLTSHKFPNITKWSEELLNHSVVKETLPPRAELVAYLQAVFGAN

Homology

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BLAST of MS026497 vs. NCBI nr
Match: XP_022151545.1 (probable glutathione S-transferase [Momordica charantia])

HSP 1 Score: 260.0 bits (663), Expect = 1.2e-65
Identity = 141/217 (64.98%), Postives = 144/217 (66.36%), Query Frame = 0


Query: 1   MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHH 60
           MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEED+WNKSDLLVKYNPIYKKVPVL+HH
Sbjct: 1   MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDJWNKSDLLVKYNPIYKKVPVLVHH 60

Query: 61  GKPIAESLVILEYIEENWKANPIFPHHPHQRALARL-----------CLLKVA------- 120
           GKPIAESLVILEYIEENWKANPIFPHHPHQRALAR             +LKVA       
Sbjct: 61  GKPIAESLVILEYIEENWKANPIFPHHPHQRALARFWAKYIDDKIVPAILKVARSKVEER 120

Query: 121 ----------------------------------------------------XLLTSHKF 148
                                                                LLTSHKF
Sbjct: 121 EKAVEEASEACEALEKELESKKLFGGDRIGLVDIVGIVVAYWVPAIEEGVGFELLTSHKF 180

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BLAST of MS026497 vs. NCBI nr
Match: KAG6603612.1 (Glutathione S-transferase U8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 193.4 bits (490), Expect = 1.4e-45
Identity = 101/191 (52.88%), Postives = 127/191 (66.49%), Query Frame = 0


Query: 1   MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDI-WNKSDLLVKYNPIYKKVPVLLH 60
           MAEELQVFGFW SPFSRRVELALKLK +EY+++EED+  NKS LL++YNPIYKKVPVL+H
Sbjct: 1   MAEELQVFGFWASPFSRRVELALKLKGVEYQHIEEDLPHNKSHLLLQYNPIYKKVPVLVH 60

Query: 61  HGKPIAESLVILEYIEENWKANPIFPHHPHQRALAR-----------LCLLKVA------ 120
           HGKPI+ESLVILEYI+E WK NP+ P +P++RALAR           + +LK A      
Sbjct: 61  HGKPISESLVILEYIDEIWKNNPLLPQNPYERALARFWAKYIEDKVVVAMLKAAKAKIED 120

Query: 121 --------------------------XLLTSHKFPNITKWSEELLNHSVVKETLPPRAEL 148
                                      +L S++FPN+ KWSEE++NH VVK+ LPP+ +L
Sbjct: 121 REKGWRKQGRRWNRLRRSLEARAVGIQILGSNRFPNVKKWSEEVVNHGVVKQILPPKDKL 180

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BLAST of MS026497 vs. NCBI nr
Match: XP_022151541.1 (probable glutathione S-transferase [Momordica charantia])

HSP 1 Score: 185.7 bits (470), Expect = 3.0e-43
Identity = 104/218 (47.71%), Postives = 125/218 (57.34%), Query Frame = 0


Query: 1   MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHH 60
           MAEE+QVFG W SPFSRRVELALKLKA+EY+Y+EED+  KSDLL+KYNP+ KKVPVL+HH
Sbjct: 1   MAEEIQVFGAWASPFSRRVELALKLKAVEYQYIEEDLTKKSDLLLKYNPLQKKVPVLVHH 60

Query: 61  GKPIAESLVILEYIEENWKAN-PIFPHHPHQRALAR------------------------ 120
           GKPIA+SLVILEYIEENWK N PIFP   H+RALAR                        
Sbjct: 61  GKPIADSLVILEYIEENWKPNYPIFPKDRHERALARFWVKYIDHKVGVASVRAARSGGEE 120

Query: 121 -----------------------------LCLLKVA-----------------XLLTSHK 148
                                        + L+ +A                  +L++ K
Sbjct: 121 REKAVETVQEALGALEKELKSKRFFGGHKIGLVDIAGIYVACWLPAFEQGLGFQILSTQK 180

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BLAST of MS026497 vs. NCBI nr
Match: XP_016900620.1 (PREDICTED: probable glutathione S-transferase [Cucumis melo] >KAA0045108.1 putative glutathione S-transferase [Cucumis melo var. makuwa] >TYK23630.1 putative glutathione S-transferase [Cucumis melo var. makuwa])

HSP 1 Score: 182.6 bits (462), Expect = 2.5e-42
Identity = 102/215 (47.44%), Postives = 125/215 (58.14%), Query Frame = 0


Query: 1   MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDI-WNKSDLLVKYNPIYKKVPVLLH 60
           MAE++QVFGFW SPFSRRVELALKLK +EY+Y EED+  NKSDLL+KYNPI+KKVPVLLH
Sbjct: 1   MAEQVQVFGFWESPFSRRVELALKLKGVEYQYFEEDLPHNKSDLLLKYNPIHKKVPVLLH 60

Query: 61  HGKPIAESLVILEYIEENWKAN-PIFPHHPHQRALAR-----------LCLLKVA----- 120
           HGKPIAESLVILEYI++ WK N PI PHHPHQRALAR           + ++K A     
Sbjct: 61  HGKPIAESLVILEYIDDVWKENYPILPHHPHQRALARFWAKFIDDKVVVAVVKAAGSRGE 120

Query: 121 -------------------------------------------------------XLLTS 143
                                                                   LLT+
Sbjct: 121 DREKAIEEAREALETLEKEIEKSNKLFGGDDIGFVDIVGTVIAGWIPAIEECFGFQLLTT 180

[Close]

BLAST of MS026497 vs. NCBI nr
Match: XP_022151543.1 (probable glutathione S-transferase [Momordica charantia])

HSP 1 Score: 182.6 bits (462), Expect = 2.5e-42
Identity = 98/210 (46.67%), Postives = 123/210 (58.57%), Query Frame = 0


Query: 6   QVFGFWTSPFSRRVELALKLKALEYEYVEEDI-WNKSDLLVKYNPIYKKVPVLLHHGKPI 65
           +VFGFW S +SRRVELALKLKA+EY+Y+EED+  NKSDLL++YNPIYKKVPVLLHHGKPI
Sbjct: 198 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 257

Query: 66  AESLVILEYIEENWKANPIFPHHPHQRALARL-----------CLLKVA----------- 125
           AESLVIL+YIE+ WKANP+ P HPHQRALAR             ++K A           
Sbjct: 258 AESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAV 317

Query: 126 ------------------------------------------------XLLTSHKFPNIT 145
                                                            +L +H FPN+T
Sbjct: 318 EEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLT 377

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BLAST of MS026497 vs. ExPASy Swiss-Prot
Match: Q03663 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 2.4e-32
Identity = 82/212 (38.68%), Postives = 109/212 (51.42%), Query Frame = 0


Query: 4   ELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHHGKP 63
           E+++ GFW SPFS RVE ALK+K ++YEY+EED  NKS LL++ NP+YKKVPVL+H+GKP
Sbjct: 3   EVKLLGFWYSPFSHRVEWALKIKGVKYEYIEEDRDNKSSLLLQSNPVYKKVPVLIHNGKP 62

Query: 64  IAESLVILEYIEENWKANPIFPHHPHQRALARLC-------------------------- 123
           I ES++ILEYI+E ++   I P  P+ RALAR                            
Sbjct: 63  IVESMIILEYIDETFEGPSILPKDPYDRALARFWAKFLDDKVAAVVNTFFRKGEEQEKGK 122

Query: 124 -----LLKVA--------------------------------------XLLTSHKFPNIT 147
                +LKV                                        L+ S KFPN +
Sbjct: 123 EEVYEMLKVLDNELKDKKFFAGDKFGFADIAANLVGFWLGVFEEGYGDVLVKSEKFPNFS 182

[Close]

BLAST of MS026497 vs. ExPASy Swiss-Prot
Match: P32110 (Probable glutathione S-transferase OS=Glycine max OX=3847 GN=HSP26-A PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 2.4e-32
Identity = 82/211 (38.86%), Postives = 108/211 (51.18%), Query Frame = 0


Query: 3   EELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHHGK 62
           E++++ G   SPF  RV++ALKLK +EY+++EE++ NKSDLL+KYNP++KKVPV +H+ +
Sbjct: 6   EDVKLLGIVGSPFVCRVQIALKLKGVEYKFLEENLGNKSDLLLKYNPVHKKVPVFVHNEQ 65

Query: 63  PIAESLVILEYIEENWKANPIFPHHPHQRALARLC------------------------- 122
           PIAESLVI+EYI+E WK NPI P  P+QRALAR                           
Sbjct: 66  PIAESLVIVEYIDETWKNNPILPSDPYQRALARFWSKFIDDKIVGAVSKSVFTVDEKERE 125

Query: 123 -----------------------------LLKVA-----------------XLLTSHKFP 143
                                        L+ +A                  L TS KFP
Sbjct: 126 KNVEETYEALQFLENELKDKKFFGGEEFGLVDIAAVFIAFWIPIFQEIAGLQLFTSEKFP 185

[Close]

BLAST of MS026497 vs. ExPASy Swiss-Prot
Match: Q9SR36 (Glutathione S-transferase U8 OS=Arabidopsis thaliana OX=3702 GN=GSTU8 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 4.2e-32
Identity = 77/216 (35.65%), Postives = 115/216 (53.24%), Query Frame = 0


Query: 3   EELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIW-NKSDLLVKYNPIYKKVPVLLHHG 62
           E +++ G W SPFS+RVE+ LKLK + YEY+EED++ N+S +L+KYNPI+KKVPVL+H+G
Sbjct: 5   EHVKLLGLWGSPFSKRVEMVLKLKGIPYEYIEEDVYGNRSPMLLKYNPIHKKVPVLIHNG 64

Query: 63  KPIAESLVILEYIEENWK-ANPIFPHHPHQRALARL------------------------ 122
           + IAESLVI+EYIE+ WK  + I P  P++RA+AR                         
Sbjct: 65  RSIAESLVIVEYIEDTWKTTHTILPQDPYERAMARFWAKYVDEKVMLAVKKACWGPESER 124

Query: 123 ------------CLLKV----------------------------------AXLLTSHKF 147
                       CL K                                     ++T+ +F
Sbjct: 125 EKEVKEAYEGLKCLEKELGDKLFFGGETIGFVDIAADFIGYWLGIFQEASGVTIMTAEEF 184

[Close]

BLAST of MS026497 vs. ExPASy Swiss-Prot
Match: Q03664 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 7.1e-32
Identity = 82/212 (38.68%), Postives = 110/212 (51.89%), Query Frame = 0


Query: 4   ELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHHGKP 63
           E+++ GFW SPF+ RVE ALKLK ++YEY+EED  NKS LL++ NP++KKVPVL+H+GKP
Sbjct: 3   EVKLLGFWYSPFTHRVEWALKLKGVKYEYIEEDRDNKSSLLLQSNPVHKKVPVLIHNGKP 62

Query: 64  IAESLVILEYIEENWKANPIFPHHPHQRALARLC-------------------------- 123
           I ES+VILEYI+E ++   I P  P+ RALAR                            
Sbjct: 63  IVESMVILEYIDETFEGPSILPKDPYDRALARFWSKFLGDKVAAVVNTFFRKGEEQEKGK 122

Query: 124 -----LLKV--------------------------------------AXLLTSHKFPNIT 147
                +LKV                                        L+TS KFPN +
Sbjct: 123 EEVYEMLKVLDNELKDKKFFVGDKFGFADIAANLVGFWLGVFEEGYGVVLVTSEKFPNFS 182

[Close]

BLAST of MS026497 vs. ExPASy Swiss-Prot
Match: Q03662 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 3.5e-31
Identity = 82/208 (39.42%), Postives = 109/208 (52.40%), Query Frame = 0


Query: 4   ELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHHGKP 63
           E+++ GFW SPFSRRVE ALK+K ++YEY+EED  NKS LL++ NPI+KKVPVL+H+GK 
Sbjct: 3   EVKLLGFWYSPFSRRVEWALKIKGVKYEYIEEDRDNKSSLLLQSNPIHKKVPVLIHNGKR 62

Query: 64  IAESLVILEYIEENWKANPIFPHHPHQRALAR---------------------------- 123
           I ES+VILEYI+E ++   I P  P+ RALAR                            
Sbjct: 63  IVESMVILEYIDETFEGPSILPKDPYDRALARFWAKFLDDKVPAVVKTFLRKGEEQEKDK 122

Query: 124 --LC-LLKV--------------------------------------AXLLTSHKFPNIT 143
             +C +LKV                                        L+TS KFPN  
Sbjct: 123 EEVCEMLKVLDNELKDKKFFVGDKFGFADIAANLVAFWLGVFEEASGVVLVTSEKFPNFC 182

[Close]

BLAST of MS026497 vs. ExPASy TrEMBL
Match: A0A6J1DBH4 (probable glutathione S-transferase OS=Momordica charantia OX=3673 GN=LOC111019459 PE=3 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 7.8e-66
Identity = 141/217 (64.98%), Postives = 144/217 (66.36%), Query Frame = 0


Query: 1   MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHH 60
           MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEED+WNKSDLLVKYNPIYKKVPVL+HH
Sbjct: 1   MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDLWNKSDLLVKYNPIYKKVPVLVHH 60

Query: 61  GKPIAESLVILEYIEENWKANPIFPHHPHQRALARL-----------CLLKVA------- 120
           GKPIAESLVILEYIEENWKANPIFPHHPHQRALAR             +LKVA       
Sbjct: 61  GKPIAESLVILEYIEENWKANPIFPHHPHQRALARFWAKYIDDKIVPAILKVARSKVEER 120

Query: 121 ----------------------------------------------------XLLTSHKF 148
                                                                LLTSHKF
Sbjct: 121 EKAVEEASEACEALEKELESKKLFGGDRIGLVDIVGIVVAYWVPAIEEGVGFELLTSHKF 180

[Close]

BLAST of MS026497 vs. ExPASy TrEMBL
Match: A0A6J1DEZ3 (probable glutathione S-transferase OS=Momordica charantia OX=3673 GN=LOC111019456 PE=3 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.4e-43
Identity = 104/218 (47.71%), Postives = 125/218 (57.34%), Query Frame = 0


Query: 1   MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHH 60
           MAEE+QVFG W SPFSRRVELALKLKA+EY+Y+EED+  KSDLL+KYNP+ KKVPVL+HH
Sbjct: 1   MAEEIQVFGAWASPFSRRVELALKLKAVEYQYIEEDLTKKSDLLLKYNPLQKKVPVLVHH 60

Query: 61  GKPIAESLVILEYIEENWKAN-PIFPHHPHQRALAR------------------------ 120
           GKPIA+SLVILEYIEENWK N PIFP   H+RALAR                        
Sbjct: 61  GKPIADSLVILEYIEENWKPNYPIFPKDRHERALARFWVKYIDHKVGVASVRAARSGGEE 120

Query: 121 -----------------------------LCLLKVA-----------------XLLTSHK 148
                                        + L+ +A                  +L++ K
Sbjct: 121 REKAVETVQEALGALEKELKSKRFFGGHKIGLVDIAGIYVACWLPAFEQGLGFQILSTQK 180

[Close]

BLAST of MS026497 vs. ExPASy TrEMBL
Match: A0A5A7TQE7 (Putative glutathione S-transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G001470 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.2e-42
Identity = 102/215 (47.44%), Postives = 125/215 (58.14%), Query Frame = 0


Query: 1   MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDI-WNKSDLLVKYNPIYKKVPVLLH 60
           MAE++QVFGFW SPFSRRVELALKLK +EY+Y EED+  NKSDLL+KYNPI+KKVPVLLH
Sbjct: 1   MAEQVQVFGFWESPFSRRVELALKLKGVEYQYFEEDLPHNKSDLLLKYNPIHKKVPVLLH 60

Query: 61  HGKPIAESLVILEYIEENWKAN-PIFPHHPHQRALAR-----------LCLLKVA----- 120
           HGKPIAESLVILEYI++ WK N PI PHHPHQRALAR           + ++K A     
Sbjct: 61  HGKPIAESLVILEYIDDVWKENYPILPHHPHQRALARFWAKFIDDKVVVAVVKAAGSRGE 120

Query: 121 -------------------------------------------------------XLLTS 143
                                                                   LLT+
Sbjct: 121 DREKAIEEAREALETLEKEIEKSNKLFGGDDIGFVDIVGTVIAGWIPAIEECFGFQLLTT 180

[Close]

BLAST of MS026497 vs. ExPASy TrEMBL
Match: A0A6J1DCG3 (probable glutathione S-transferase OS=Momordica charantia OX=3673 GN=LOC111019458 PE=4 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.2e-42
Identity = 98/210 (46.67%), Postives = 123/210 (58.57%), Query Frame = 0


Query: 6   QVFGFWTSPFSRRVELALKLKALEYEYVEEDI-WNKSDLLVKYNPIYKKVPVLLHHGKPI 65
           +VFGFW S +SRRVELALKLKA+EY+Y+EED+  NKSDLL++YNPIYKKVPVLLHHGKPI
Sbjct: 198 EVFGFWESAYSRRVELALKLKAVEYQYIEEDLPHNKSDLLLRYNPIYKKVPVLLHHGKPI 257

Query: 66  AESLVILEYIEENWKANPIFPHHPHQRALARL-----------CLLKVA----------- 125
           AESLVIL+YIE+ WKANP+ P HPHQRALAR             ++K A           
Sbjct: 258 AESLVILQYIEDTWKANPMLPQHPHQRALARFWAKYIDDKVVGAVVKAAVSKGEEREKAV 317

Query: 126 ------------------------------------------------XLLTSHKFPNIT 145
                                                            +L +H FPN+T
Sbjct: 318 EEAREALEELEKELKSKKFFGGHNIGFVDIVGTIVAHWVPAIEQSFGFEVLKAHNFPNLT 377

[Close]

BLAST of MS026497 vs. ExPASy TrEMBL
Match: A0A6J1DCG3 (probable glutathione S-transferase OS=Momordica charantia OX=3673 GN=LOC111019458 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 4.6e-42
Identity = 86/95 (90.53%), Postives = 89/95 (93.68%), Query Frame = 0


Query: 1  MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHH 60
          MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEED  NKSDLLVKYNPIYKKVPVLLHH
Sbjct: 1  MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDXRNKSDLLVKYNPIYKKVPVLLHH 60

Query: 61 GKPIAESLVILEYIEENWKANPIFPHHPHQRALAR 96
          GKPIAESLVILEYIE+ WK+NP+FP HPH  ALAR
Sbjct: 61 GKPIAESLVILEYIEDTWKSNPMFPQHPHGTALAR 95

HSP 2 Score: 182.6 bits (462), Expect = 1.2e-42
Identity = 102/215 (47.44%), Postives = 125/215 (58.14%), Query Frame = 0


Query: 1   MAEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDI-WNKSDLLVKYNPIYKKVPVLLH 60
           MAE++QVFGFW SPFSRRVELALKLK +EY+Y EED+  NKSDLL+KYNPI+KKVPVLLH
Sbjct: 1   MAEQVQVFGFWESPFSRRVELALKLKGVEYQYFEEDLPHNKSDLLLKYNPIHKKVPVLLH 60

Query: 61  HGKPIAESLVILEYIEENWKAN-PIFPHHPHQRALAR-----------LCLLKVA----- 120
           HGKPIAESLVILEYI++ WK N PI PHHPHQRALAR           + ++K A     
Sbjct: 61  HGKPIAESLVILEYIDDVWKENYPILPHHPHQRALARFWAKFIDDKVVVAVVKAAGSRGE 120

Query: 121 -------------------------------------------------------XLLTS 143
                                                                   LLT+
Sbjct: 121 DREKAIEEAREALETLEKEIEKSNKLFGGDDIGFVDIVGTVIAGWIPAIEECFGFQLLTT 180

[Close]

BLAST of MS026497 vs. TAIR 10
Match: AT3G09270.1 (glutathione S-transferase TAU 8 )

HSP 1 Score: 139.0 bits (349), Expect = 3.0e-33
Identity = 77/216 (35.65%), Postives = 115/216 (53.24%), Query Frame = 0


Query: 3   EELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIW-NKSDLLVKYNPIYKKVPVLLHHG 62
           E +++ G W SPFS+RVE+ LKLK + YEY+EED++ N+S +L+KYNPI+KKVPVL+H+G
Sbjct: 5   EHVKLLGLWGSPFSKRVEMVLKLKGIPYEYIEEDVYGNRSPMLLKYNPIHKKVPVLIHNG 64

Query: 63  KPIAESLVILEYIEENWK-ANPIFPHHPHQRALARL------------------------ 122
           + IAESLVI+EYIE+ WK  + I P  P++RA+AR                         
Sbjct: 65  RSIAESLVIVEYIEDTWKTTHTILPQDPYERAMARFWAKYVDEKVMLAVKKACWGPESER 124

Query: 123 ------------CLLKV----------------------------------AXLLTSHKF 147
                       CL K                                     ++T+ +F
Sbjct: 125 EKEVKEAYEGLKCLEKELGDKLFFGGETIGFVDIAADFIGYWLGIFQEASGVTIMTAEEF 184

[Close]

BLAST of MS026497 vs. TAIR 10
Match: AT2G29420.1 (glutathione S-transferase tau 7 )

HSP 1 Score: 131.7 bits (330), Expect = 4.7e-31
Identity = 55/94 (58.51%), Postives = 78/94 (82.98%), Query Frame = 0


Query: 2   AEELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHHG 61
           +EE+++ G W SPFSRR+E+AL LK + YE++E+DI NKS LL++ NP++K +PVL+H+G
Sbjct: 7   SEEVKLLGMWASPFSRRIEIALTLKGVSYEFLEQDITNKSSLLLQLNPVHKMIPVLVHNG 66

Query: 62  KPIAESLVILEYIEENWKANPIFPHHPHQRALAR 96
           KPI+ESLVILEYI+E W+ NPI P  P++R +AR
Sbjct: 67  KPISESLVILEYIDETWRDNPILPQDPYERTMAR 100

[Close]

BLAST of MS026497 vs. TAIR 10
Match: AT2G29440.1 (glutathione S-transferase tau 6 )

HSP 1 Score: 130.2 bits (326), Expect = 1.4e-30
Identity = 61/113 (53.98%), Postives = 82/113 (72.57%), Query Frame = 0


Query: 3   EELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHHGK 62
           EE+++ G W SPFSRR+E+ALKLK + YEY+EED+ NKS LL+  +PI+KK+PVL+H+GK
Sbjct: 5   EEVKLLGIWASPFSRRIEMALKLKGVPYEYLEEDLENKSSLLLALSPIHKKIPVLVHNGK 64

Query: 63  PIAESLVILEYIEENWKANPIFPHHPHQRALARLCLLKVAXLLTSHKFPNITK 116
            I ES VILEYI+E WK NPI P  P QR+ AR+    V   + +  F ++ K
Sbjct: 65  TIIESHVILEYIDETWKHNPILPQDPFQRSKARVLAKLVDEKIVNVGFASLAK 117

[Close]

BLAST of MS026497 vs. TAIR 10
Match: AT2G29450.1 (glutathione S-transferase tau 5 )

HSP 1 Score: 128.6 bits (322), Expect = 4.0e-30
Identity = 68/139 (48.92%), Postives = 92/139 (66.19%), Query Frame = 0


Query: 3   EELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHHGK 62
           EE+++ G W SPFSRRVE+ALKLK + YEYVEE + NKS LL+  NPI+KKVPVL+H+GK
Sbjct: 5   EEVKLLGIWASPFSRRVEMALKLKGIPYEYVEEILENKSPLLLALNPIHKKVPVLVHNGK 64

Query: 63  PIAESLVILEYIEENWKANPIFPHHPHQRALARLCLLKVAXLLTSHKFPNITKWSEELLN 122
            I ES VILEYI+E W  NPI P  P++R+ AR     V   + +  F ++ +  E+   
Sbjct: 65  TILESHVILEYIDETWPQNPILPQDPYERSKARFFAKLVDEQIMNVGFISMARADEK--G 124

Query: 123 HSVVKETLPPRAELVAYLQ 142
             V+ E +    EL+ YL+
Sbjct: 125 REVLAEQV---RELIMYLE 138

[Close]

BLAST of MS026497 vs. TAIR 10
Match: AT2G29480.1 (glutathione S-transferase tau 2 )

HSP 1 Score: 124.8 bits (312), Expect = 5.8e-29
Identity = 54/93 (58.06%), Postives = 75/93 (80.65%), Query Frame = 0


Query: 3  EELQVFGFWTSPFSRRVELALKLKALEYEYVEEDIWNKSDLLVKYNPIYKKVPVLLHHGK 62
          E +++ GFW SPFSRRVE+ALKLK + YEY+EED+  KS LL++ NP++KKVPVL+H+ K
Sbjct: 6  ESVKLLGFWISPFSRRVEMALKLKGVPYEYLEEDLPKKSTLLLELNPVHKKVPVLVHNDK 65

Query: 63 PIAESLVILEYIEENWKANPIFPHHPHQRALAR 96
           ++ES VILEYI++ W  NPI PH P+++A+ R
Sbjct: 66 LLSESHVILEYIDQTWNNNPILPHDPYEKAMVR 98

The following BLAST results are available for this feature:

BLAST of MS026497 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151545.1	1.2e-65	64.98	probable glutathione S-transferase [Momordica charantia]	[more]
KAG6603612.1	1.4e-45	52.88	Glutathione S-transferase U8, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022151541.1	3.0e-43	47.71	probable glutathione S-transferase [Momordica charantia]	[more]
XP_016900620.1	2.5e-42	47.44	PREDICTED: probable glutathione S-transferase [Cucumis melo] >KAA0045108.1 putat...	[more]
XP_022151543.1	2.5e-42	46.67	probable glutathione S-transferase [Momordica charantia]	[more]

Pages

BLAST of MS026497 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q03663	2.4e-32	38.68	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P32110	2.4e-32	38.86	Probable glutathione S-transferase OS=Glycine max OX=3847 GN=HSP26-A PE=2 SV=1	[more]
Q9SR36	4.2e-32	35.65	Glutathione S-transferase U8 OS=Arabidopsis thaliana OX=3702 GN=GSTU8 PE=2 SV=1	[more]
Q03664	7.1e-32	38.68	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q03662	3.5e-31	39.42	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]

BLAST of MS026497 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBH4	7.8e-66	64.98	probable glutathione S-transferase OS=Momordica charantia OX=3673 GN=LOC11101945...	[more]
A0A6J1DEZ3	1.4e-43	47.71	probable glutathione S-transferase OS=Momordica charantia OX=3673 GN=LOC11101945...	[more]
A0A5A7TQE7	1.2e-42	47.44	Putative glutathione S-transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1DCG3	1.2e-42	46.67	probable glutathione S-transferase OS=Momordica charantia OX=3673 GN=LOC11101945...	[more]
A0A6J1DCG3	4.6e-42	90.53	probable glutathione S-transferase OS=Momordica charantia OX=3673 GN=LOC11101945...	[more]

BLAST of MS026497 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09270.1	3.0e-33	35.65	glutathione S-transferase TAU 8	[more]
AT2G29420.1	4.7e-31	58.51	glutathione S-transferase tau 7	[more]
AT2G29440.1	1.4e-30	53.98	glutathione S-transferase tau 6	[more]
AT2G29450.1	4.0e-30	48.92	glutathione S-transferase tau 5	[more]
AT2G29480.1	5.8e-29	58.06	glutathione S-transferase tau 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.1050.10		coord: 92..145 e-value: 2.2E-8 score: 36.1
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 1..86 e-value: 2.2E-39 score: 135.3
None	No IPR available	PANTHER	PTHR11260	GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING	coord: 1..96
None	No IPR available	PANTHER	PTHR11260	GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING	coord: 100..143
None	No IPR available	PANTHER	PTHR11260:SF676	GLUTATHIONE S-TRANSFERASE U8	coord: 100..143
None	No IPR available	PANTHER	PTHR11260:SF676	GLUTATHIONE S-TRANSFERASE U8	coord: 1..96
None	No IPR available	CDD	cd03058	GST_N_Tau	coord: 5..78 e-value: 3.65074E-40 score: 127.779
IPR004045	Glutathione S-transferase, N-terminal	PFAM	PF02798	GST_N	coord: 4..75 e-value: 1.7E-17 score: 63.5
IPR004045	Glutathione S-transferase, N-terminal	PROSITE	PS50404	GST_NTER	coord: 3..82 score: 20.860605
IPR040079	Glutathione Transferase family	SFLD	SFLDS00019	Glutathione_Transferase_(cyto	coord: 6..102 e-value: 4.7E-21 score: 70.1
IPR036282	Glutathione S-transferase, C-terminal domain superfamily	SUPERFAMILY	47616	GST C-terminal domain-like	coord: 76..143
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 5..98

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS026497.1	MS026497.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006749	glutathione metabolic process
molecular_function	GO:0005515	protein binding