MS026484 (gene) Bitter gourd (TR) v1

Overview
NameMS026484
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionserine/threonine-protein kinase STN8, chloroplastic
Locationscaffold607: 304939 .. 308661 (-)
RNA-Seq ExpressionMS026484
SyntenyMS026484
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCTCTCCTCTCCCCACTTTCGATTTCCCTCCAAATCACTCCCCAAACCCTCTTTTTCTCTCCTCTCAAATCCCACGTCTCCCTCTTCGCCGGAACTTTCAGGACCAAGGCATTCTTCGGCGACATCTCCGACCGCCTGCTCGATAACTTGGACGGCCCTCTCCGGCCGGTGGATCAGCTTCCGGTTTTGCAATCTGGGTACCTTCAGTTTCAACGATTCTCGGGGGAATTGAGCGATTTGCAGAAATGGGGTTTCATTGTTTTTGCTGGGGTTTTGTGGGTTTATCTCACTGCTCGACCAGGGGTGCTTTTTGGCGCCATTGATGCCTATCTTCTGGCTCCTCTGCAGCTGGGGCTCGACAGTTTGACGGGGAGGAGAAGCTTGAAGAGCTCTGATTTTGTGATTGGGGGAAAATTGGGGGAGGGTTCGTTTGGAGTTGTTTATTCGGGTGCGTTTCTTCCCAAGAATGTGAAGATTGAGGAGCGAGCGAGTAGAGGTTTGGAGGTGGATGGGAAGGACAAGGTGATTCTTAAGAAGGTAACACCAGGCTGCGTTCTAGTAGTGTTTTTCTATGATGTGATGCAAAAGTGCTTTTTCAATAAGAAAATTTTTTCTGCTAGCACTTAAGAAGTACTTAATTTAAAGTGTGTTTTGTAGAGTTTCTTTAAGTGATGATGATTCTCAATGTGACTTTAAAATGATTCACACCTATAGTTTTAAGCATTTAAGCATTTAAGAGATGATTATGAGAAAAATGATTGAACTAAAAGATATGTCGTCACTATTTTTAGGAAAAGTACTTTAATTATAAAAATTTTAGCAAACACTTTGATGTTTAGCACTGTCAAACACATGAAGCCAAAAGTCAACAAAGGCTTTTCCCATTTTCAAAAATACTCGTGGGTTAGTTTAGTACAAACTTGTGCTTCTTGGCCAAGCACTTGACCTTAAATTATTCTAGGAGTGTTACTTTATAAAAGCCCTTTTGGGTGCTTTTACCATTTTTAAAGAACACTTGTATATTACTCTCAAACACCATGATTTTGAAAGAGAAGTGCTTGTTGCTTATTATTTGAGCAATAGAATGCTATCCCTGACTTCTGCTATTTCGATTTCCATGTGCTTTTTAGGTATGAAAAAGAACAAATGAAGATGCGTTTTTCTTGTTTTTGTTTTGTTTGGGGGTAATGGTGCAGATTAAGGTGGGAGTTAAAGGAGCAGAGAAGTTTGGGGAATATGAAGAGTGGTTTAACTACAGACTTTCAAGAGCAGCGCCTGAAACTTGTGCTGAGTTTCTTGGCAGTTTTGTAGCTGATAGAGAAAGCAGGCAATTCTCTGCAGGAGGGAAATGGCTCGTTTGGAAATTTGAGGTACTTTACACATCATTTACTGTAACAATTTAATCATTCCATATATTATCGTCTTGAATTATGACACAAGTATATGAAATGGAGAGGAAGTGCTGTTGTAAGCTTTTGAACTCGAGATCTCCTAATAACATTTTGTAAAAGTTCAAGCGACATCGAAAGTTTTTCAGGTAAAGACGTCCTGATAACATTAGATCTGAGGTAGAGAAAATAAGGAAAGGCCTTAGCTTGTCTTAGATTCAAATATTATATAAAAACTCAGGTTTAAGGGCTTGTTGGGTAATGTTATTGTTTATTGTTTATTGTTTATTCTTTTTTAATAAATAAAAATAGAACTGAATTTGATAATTTTTTCTTGTTTCCTGGTCTTTTTCTTGTAATTGAAAAAATAAACAACAACATGTTTGGTAATAATGTGTTGATTTCTGTTATCTTCCATTTGAAATAAAAAAATAAAAGTAAAAAGTTTTATAAGTATTTAATTTTTTAAAATTAAATTATGCTATATTTATTTTTTTTAAATATTTATTATGCATTTTATAAGTCAAGGTGAAGGTTTAATTTACACTATGAAAAAAATTAGAAACTATAACAAGAAACAAGAAACTCGATTTTGTTGTTGCTAAAATTTTTATACAATTTTGGAAAAGTTTCATGAAAATGAGAAACGAGAAACAGGAATAGTTATCAAACACTGTAGTTCTAAATTGGAAATAAGAAACAAGAAACGGGAAAAGTTACTAGTCGGGTCTAAAGTTCTCTCTTTAATTTGTTAGTTAAGATGTAAAATTTTACCACTTTTATGATAATTTTCGGCTCCACTTGACACTAAACATAGTAAAAGTTAAGTTTAAGTTATTGGGCTAGAGGAAATTTGTAATACATCTCAATCAATCTAAACTCTTAATTTTATTATTATGAATCAAGTTGGAGGCAACATTCAAAACCAATTTCTCTGATCCCATCTGAATATGACAGGGAGATCAAACTCTTGCTGATTACATGAAAGATAGAAGCTTCCCTGTAAACTTGGAAGCTCTTATGTTTGGACGTGTCTTACGAGGCGTAAACTCAGTCGAGAAGAACGCTTTGATAATCAAGCAAATCTTACGCCAAATCATTAAATCATTGAAGAAGATCCACGACACAGGTATTGTTCACAGGGATGTGAAGCCTGCAAACTTAGTAGTAACCAAGAAAGGGCAGATAAAGATTATTGATTTCGGGGCAGCCACTGACCTTCGGATCGGCAAGAACTACGTACCAAATCTTGCTTTGCTGGATCCCGACTACTGCCCTCCCGAGCTTTACGTGATGCCTGAAGAAACACCCAGCCCCCCTCCAGCTCCCATTGCTGCACTTTTTTCTCCCATAGTCTGGCAGGTATGCTGAGTTCCATGGATCTGTTCTTAAAAGCCTAAGTTTGGGAATCTGATGCCTTTTAGTAAAGTGATAAAAGAGTTTAATTATGAATATTTGAAGTAAGCTCAGTTTATATCTATTTATATAGAAGGTTAATTTACAAGTTTTAGTTCCTAAATGATAGGGTTGTGTCTATTACGTTATTGAACTTAAAAAAAAAAGGTTTTCTAATAGGTTCTAAACTTCTAAAAGTTTGCCTACTTGGCTCAATAGCTTTAAAAATTTTAAATAGATTTTTAACTTTCAATTTTATCTCTAAAAGGCCTCAATCACATTTTAACTGTATTATGTATTAGTTAAAGATTAGATGGTGGTTGAATTTTAGGCCAGGTCAAGTAAACTGAGGCATAATCATAAACTAATAGAGTTAGCATCTGCACAAAGTTCAAGAATCTATTAGAAACTTATTAAAGTTCAAAAGGGACTTATTTAAAATTTTTTAAAGTACAAAAACGGAGTAGATACAATACAACATACAGGGACCAAATTCATGATTTATGCTGTTAAAAGGGCTGTTGTGGAATTCCTTTGCATATTTTTGAATTATTTCAATTTGGAAACTGTTTTGGTTTTGCAGCTGAACAGCCCAGATTTGTTTGACATGTATTCTGCTGGTATTGTGCTTTTGCAAATGGCAGTACCAAACTTGAGAAGTAGTGCTGGGTTGAAGAATTTCAATTTGGAACTCAAGAATTATGGATATGACTTGAATAAATGGAGGGAGAAAACAAGAACAAAGCCCGATTTGACGATTCTCGATCTCGACTCGGGGAGGGGGTGGGATCTCGCAACGAAGCTTGTCTCGGAGAGAGGCTTCCTTAGAAGGGGGCGTTTATCGGCTGCCGCTGCTCTGAGGCATCCTTATTTCTTGTTGGGTGGTGATCAGGCAGCTGCAGTTATTTCAAAACTAAATTTGGTCAAGAAATGA

mRNA sequence

ATGGCCTCTCTCCTCTCCCCACTTTCGATTTCCCTCCAAATCACTCCCCAAACCCTCTTTTTCTCTCCTCTCAAATCCCACGTCTCCCTCTTCGCCGGAACTTTCAGGACCAAGGCATTCTTCGGCGACATCTCCGACCGCCTGCTCGATAACTTGGACGGCCCTCTCCGGCCGGTGGATCAGCTTCCGGTTTTGCAATCTGGGTACCTTCAGTTTCAACGATTCTCGGGGGAATTGAGCGATTTGCAGAAATGGGGTTTCATTGTTTTTGCTGGGGTTTTGTGGGTTTATCTCACTGCTCGACCAGGGGTGCTTTTTGGCGCCATTGATGCCTATCTTCTGGCTCCTCTGCAGCTGGGGCTCGACAGTTTGACGGGGAGGAGAAGCTTGAAGAGCTCTGATTTTGTGATTGGGGGAAAATTGGGGGAGGGTTCGTTTGGAGTTGTTTATTCGGGTGCGTTTCTTCCCAAGAATGTGAAGATTGAGGAGCGAGCGAGTAGAGGTTTGGAGGTGGATGGGAAGGACAAGGTGATTCTTAAGAAGATTAAGGTGGGAGTTAAAGGAGCAGAGAAGTTTGGGGAATATGAAGAGTGGTTTAACTACAGACTTTCAAGAGCAGCGCCTGAAACTTGTGCTGAGTTTCTTGGCAGTTTTGTAGCTGATAGAGAAAGCAGGCAATTCTCTGCAGGAGGGAAATGGCTCGTTTGGAAATTTGAGGGAGATCAAACTCTTGCTGATTACATGAAAGATAGAAGCTTCCCTGTAAACTTGGAAGCTCTTATGTTTGGACGTGTCTTACGAGGCGTAAACTCAGTCGAGAAGAACGCTTTGATAATCAAGCAAATCTTACGCCAAATCATTAAATCATTGAAGAAGATCCACGACACAGGTATTGTTCACAGGGATGTGAAGCCTGCAAACTTAGTAGTAACCAAGAAAGGGCAGATAAAGATTATTGATTTCGGGGCAGCCACTGACCTTCGGATCGGCAAGAACTACGTACCAAATCTTGCTTTGCTGGATCCCGACTACTGCCCTCCCGAGCTTTACGTGATGCCTGAAGAAACACCCAGCCCCCCTCCAGCTCCCATTGCTGCACTTTTTTCTCCCATAGTCTGGCAGCTGAACAGCCCAGATTTGTTTGACATGTATTCTGCTGGTATTGTGCTTTTGCAAATGGCAGTACCAAACTTGAGAAGTAGTGCTGGGTTGAAGAATTTCAATTTGGAACTCAAGAATTATGGATATGACTTGAATAAATGGAGGGAGAAAACAAGAACAAAGCCCGATTTGACGATTCTCGATCTCGACTCGGGGAGGGGGTGGGATCTCGCAACGAAGCTTGTCTCGGAGAGAGGCTTCCTTAGAAGGGGGCGTTTATCGGCTGCCGCTGCTCTGAGGCATCCTTATTTCTTGTTGGGTGGTGATCAGGCAGCTGCAGTTATTTCAAAACTAAATTTGGTCAAGAAATGA

Coding sequence (CDS)

ATGGCCTCTCTCCTCTCCCCACTTTCGATTTCCCTCCAAATCACTCCCCAAACCCTCTTTTTCTCTCCTCTCAAATCCCACGTCTCCCTCTTCGCCGGAACTTTCAGGACCAAGGCATTCTTCGGCGACATCTCCGACCGCCTGCTCGATAACTTGGACGGCCCTCTCCGGCCGGTGGATCAGCTTCCGGTTTTGCAATCTGGGTACCTTCAGTTTCAACGATTCTCGGGGGAATTGAGCGATTTGCAGAAATGGGGTTTCATTGTTTTTGCTGGGGTTTTGTGGGTTTATCTCACTGCTCGACCAGGGGTGCTTTTTGGCGCCATTGATGCCTATCTTCTGGCTCCTCTGCAGCTGGGGCTCGACAGTTTGACGGGGAGGAGAAGCTTGAAGAGCTCTGATTTTGTGATTGGGGGAAAATTGGGGGAGGGTTCGTTTGGAGTTGTTTATTCGGGTGCGTTTCTTCCCAAGAATGTGAAGATTGAGGAGCGAGCGAGTAGAGGTTTGGAGGTGGATGGGAAGGACAAGGTGATTCTTAAGAAGATTAAGGTGGGAGTTAAAGGAGCAGAGAAGTTTGGGGAATATGAAGAGTGGTTTAACTACAGACTTTCAAGAGCAGCGCCTGAAACTTGTGCTGAGTTTCTTGGCAGTTTTGTAGCTGATAGAGAAAGCAGGCAATTCTCTGCAGGAGGGAAATGGCTCGTTTGGAAATTTGAGGGAGATCAAACTCTTGCTGATTACATGAAAGATAGAAGCTTCCCTGTAAACTTGGAAGCTCTTATGTTTGGACGTGTCTTACGAGGCGTAAACTCAGTCGAGAAGAACGCTTTGATAATCAAGCAAATCTTACGCCAAATCATTAAATCATTGAAGAAGATCCACGACACAGGTATTGTTCACAGGGATGTGAAGCCTGCAAACTTAGTAGTAACCAAGAAAGGGCAGATAAAGATTATTGATTTCGGGGCAGCCACTGACCTTCGGATCGGCAAGAACTACGTACCAAATCTTGCTTTGCTGGATCCCGACTACTGCCCTCCCGAGCTTTACGTGATGCCTGAAGAAACACCCAGCCCCCCTCCAGCTCCCATTGCTGCACTTTTTTCTCCCATAGTCTGGCAGCTGAACAGCCCAGATTTGTTTGACATGTATTCTGCTGGTATTGTGCTTTTGCAAATGGCAGTACCAAACTTGAGAAGTAGTGCTGGGTTGAAGAATTTCAATTTGGAACTCAAGAATTATGGATATGACTTGAATAAATGGAGGGAGAAAACAAGAACAAAGCCCGATTTGACGATTCTCGATCTCGACTCGGGGAGGGGGTGGGATCTCGCAACGAAGCTTGTCTCGGAGAGAGGCTTCCTTAGAAGGGGGCGTTTATCGGCTGCCGCTGCTCTGAGGCATCCTTATTTCTTGTTGGGTGGTGATCAGGCAGCTGCAGTTATTTCAAAACTAAATTTGGTCAAGAAATGA

Protein sequence

MASLLSPLSISLQITPQTLFFSPLKSHVSLFAGTFRTKAFFGDISDRLLDNLDGPLRPVDQLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGAIDAYLLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRGLEVDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSAGGKWLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKSLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFNLELKNYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRHPYFLLGGDQAAAVISKLNLVKK
Homology
BLAST of MS026484 vs. NCBI nr
Match: XP_022144101.1 (serine/threonine-protein kinase STN8, chloroplastic [Momordica charantia])

HSP 1 Score: 970.3 bits (2507), Expect = 6.2e-279
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MASLLSPLSISLQITPQTLFFSPLKSHVSLFAGTFRTKAFFGDISDRLLDNLDGPLRPVD 60
           MASLLSPLSISLQITPQTLFFSPLKSHVSLFAGTFRTKAFFGDISDRLLDNLDGPLRPVD
Sbjct: 1   MASLLSPLSISLQITPQTLFFSPLKSHVSLFAGTFRTKAFFGDISDRLLDNLDGPLRPVD 60

Query: 61  QLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGAIDAYLLAPLQLG 120
           QLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGAIDAYLLAPLQLG
Sbjct: 61  QLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGAIDAYLLAPLQLG 120

Query: 121 LDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRGLEVDGKDKVILK 180
           LDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRGLEVDGKDKVILK
Sbjct: 121 LDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRGLEVDGKDKVILK 180

Query: 181 KIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSAGGKWLVWKFEG 240
           KIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSAGGKWLVWKFEG
Sbjct: 181 KIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSAGGKWLVWKFEG 240

Query: 241 DQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKSLKKIHDTGIVH 300
           DQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKSLKKIHDTGIVH
Sbjct: 241 DQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKSLKKIHDTGIVH 300

Query: 301 RDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPP 360
           RDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPP
Sbjct: 301 RDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPP 360

Query: 361 PAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFNLELKNYGYDLNK 420
           PAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFNLELKNYGYDLNK
Sbjct: 361 PAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFNLELKNYGYDLNK 420

Query: 421 WREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRHPYFLLGGDQAAA 480
           WREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRHPYFLLGGDQAAA
Sbjct: 421 WREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRHPYFLLGGDQAAA 480

Query: 481 VISKLNLVKK 491
           VISKLNLVKK
Sbjct: 481 VISKLNLVKK 490

BLAST of MS026484 vs. NCBI nr
Match: XP_022950141.1 (serine/threonine-protein kinase STN8, chloroplastic [Cucurbita moschata])

HSP 1 Score: 854.7 bits (2207), Expect = 3.8e-244
Identity = 437/502 (87.05%), Postives = 463/502 (92.23%), Query Frame = 0

Query: 1   MASLLSPLSISLQITPQTLFFSPLK----SHVSLFAGTFRTK--------AFFGDISDRL 60
           MASL+ P +ISLQ T QT  FSPLK    + + LFAGTFR K        +FFGDISD L
Sbjct: 1   MASLIFPFAISLQQTHQTR-FSPLKPILPTEIPLFAGTFRPKPPAGKCRASFFGDISDDL 60

Query: 61  LDNLDGPLRPVDQLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGA 120
           L+NLDGPLR VDQLPVLQSGY+QFQRFSGELSDLQKWGFIVFAG +WVYLTARPGVLFGA
Sbjct: 61  LNNLDGPLR-VDQLPVLQSGYVQFQRFSGELSDLQKWGFIVFAGFIWVYLTARPGVLFGA 120

Query: 121 IDAYLLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRG 180
           ID YLLAPLQLGL SL GRR+LKSSDFVIG KLGEGSFGVVYSGAFLPKNVKIE R S+ 
Sbjct: 121 IDTYLLAPLQLGLYSLIGRRNLKSSDFVIGSKLGEGSFGVVYSGAFLPKNVKIENRTSKV 180

Query: 181 LEVDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFS 240
           LE+DGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRE+RQFS
Sbjct: 181 LELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRENRQFS 240

Query: 241 AGGKWLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIK 300
           AGGKWLVWKFEGDQTL DYMKDRSFP+NLE LMF RVL+G+NS+E+NALIIKQILRQII 
Sbjct: 241 AGGKWLVWKFEGDQTLGDYMKDRSFPLNLETLMFRRVLQGMNSIERNALIIKQILRQIIT 300

Query: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE 360
           SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNY+PNLALLDPDYCPPE
Sbjct: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYIPNLALLDPDYCPPE 360

Query: 361 LYVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFN 420
           LYVMPEETPSPPPAPIAAL SPI+WQLNSPDLFDMYSAGIVL+QMAVP+LRSSAGLKNFN
Sbjct: 361 LYVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPSLRSSAGLKNFN 420

Query: 421 LELKNYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRH 480
           +E+KNYGYDLN+WRE+TRTKPDLTILDLDS RGWDLATKLVSERG LRRGRLSA+AALRH
Sbjct: 421 MEIKNYGYDLNEWRERTRTKPDLTILDLDSRRGWDLATKLVSERGSLRRGRLSASAALRH 480

Query: 481 PYFLLGGDQAAAVISKLNLVKK 491
           PYFLLG DQAAAVISKLNLVKK
Sbjct: 481 PYFLLGSDQAAAVISKLNLVKK 500

BLAST of MS026484 vs. NCBI nr
Match: XP_023545193.1 (serine/threonine-protein kinase STN8, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 854.7 bits (2207), Expect = 3.8e-244
Identity = 437/502 (87.05%), Postives = 463/502 (92.23%), Query Frame = 0

Query: 1   MASLLSPLSISLQITPQTLFFSPLK----SHVSLFAGTFRTK--------AFFGDISDRL 60
           MASL+ P +ISLQ T QT  FSPLK    + + LFAGTFR K        +FFGDISD L
Sbjct: 1   MASLIFPFAISLQQTHQTR-FSPLKPILPTEIPLFAGTFRPKPPAGKCRASFFGDISDDL 60

Query: 61  LDNLDGPLRPVDQLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGA 120
           L+NLDGPLR VDQLPVLQSGY+QFQRFSGELSDLQKWGFIVFAG +WVYLTARPGVLFGA
Sbjct: 61  LNNLDGPLR-VDQLPVLQSGYVQFQRFSGELSDLQKWGFIVFAGFIWVYLTARPGVLFGA 120

Query: 121 IDAYLLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRG 180
           ID YLLAPLQLGL SL GRR+LKSSDFVIG KLGEGSFGVVYSGAFLPKNVKIE R S+ 
Sbjct: 121 IDTYLLAPLQLGLYSLIGRRNLKSSDFVIGSKLGEGSFGVVYSGAFLPKNVKIENRTSKV 180

Query: 181 LEVDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFS 240
           LE+DGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRE+RQFS
Sbjct: 181 LELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRENRQFS 240

Query: 241 AGGKWLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIK 300
           AGGKWLVWKFEGDQTL DYMKDRSFP+NLE LMF RVL+G+NS+E+NALIIKQILRQII 
Sbjct: 241 AGGKWLVWKFEGDQTLGDYMKDRSFPLNLETLMFRRVLQGMNSIERNALIIKQILRQIIT 300

Query: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE 360
           SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE
Sbjct: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE 360

Query: 361 LYVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFN 420
           LYVMPEETPSPPPAPIAAL SPI+WQLNSPDLFDMYSAGIVL+QMAVP+LRSSAGLKNFN
Sbjct: 361 LYVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPSLRSSAGLKNFN 420

Query: 421 LELKNYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRH 480
           +E+KNYGYDLN+WRE+TRTKPDLTILDLDS RGWDLATKL+SERG LRRGRLSA+AALRH
Sbjct: 421 MEIKNYGYDLNEWRERTRTKPDLTILDLDSRRGWDLATKLISERGSLRRGRLSASAALRH 480

Query: 481 PYFLLGGDQAAAVISKLNLVKK 491
           PYFLLG DQAAAVISKLNLVKK
Sbjct: 481 PYFLLGSDQAAAVISKLNLVKK 500

BLAST of MS026484 vs. NCBI nr
Match: KAG6603530.1 (Serine/threonine-protein kinase STN8, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 853.2 bits (2203), Expect = 1.1e-243
Identity = 437/502 (87.05%), Postives = 462/502 (92.03%), Query Frame = 0

Query: 1   MASLLSPLSISLQITPQTLFFSPLK----SHVSLFAGTFRTK--------AFFGDISDRL 60
           MASL+ P +ISLQ T QT  FSPLK    + + LFAGTFR K        +FFGDISD L
Sbjct: 1   MASLIFPFAISLQQTHQTR-FSPLKPILPTEIPLFAGTFRPKPPSGKCRASFFGDISDDL 60

Query: 61  LDNLDGPLRPVDQLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGA 120
           L+NLDGPLR VDQLPVLQSGY+QFQRFSGELSDLQKWGFIVFAG +WVYLTARPGVLFGA
Sbjct: 61  LNNLDGPLR-VDQLPVLQSGYVQFQRFSGELSDLQKWGFIVFAGFIWVYLTARPGVLFGA 120

Query: 121 IDAYLLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRG 180
           ID YLLAPLQLGL SL GRR+LKSSDFVIG KLGEGSFGVVYSGAFLPKNVKIE R S+ 
Sbjct: 121 IDTYLLAPLQLGLYSLIGRRNLKSSDFVIGSKLGEGSFGVVYSGAFLPKNVKIENRTSKV 180

Query: 181 LEVDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFS 240
           LE+DGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRE+RQFS
Sbjct: 181 LELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRENRQFS 240

Query: 241 AGGKWLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIK 300
           AGGKWLVWKFEGDQTL DYMKDRSFP+NLE LMF RVL+G+NS+E+NALIIKQILRQII 
Sbjct: 241 AGGKWLVWKFEGDQTLGDYMKDRSFPLNLETLMFRRVLQGMNSIERNALIIKQILRQIIT 300

Query: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE 360
           SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE
Sbjct: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE 360

Query: 361 LYVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFN 420
           LYVMPEETPSPPPAPIAAL SPI+WQLNSPDLFDMYSAGIVL+QMAVP+LRSSAGLKNFN
Sbjct: 361 LYVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPSLRSSAGLKNFN 420

Query: 421 LELKNYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRH 480
           +E+KNYGYDLN+WRE+TRTKPDLTILDLDS RGWDLATKLVSERG LRRGRLSA+AALRH
Sbjct: 421 MEIKNYGYDLNEWRERTRTKPDLTILDLDSRRGWDLATKLVSERGSLRRGRLSASAALRH 480

Query: 481 PYFLLGGDQAAAVISKLNLVKK 491
           PYFLLG DQAAAVISKLNLV K
Sbjct: 481 PYFLLGSDQAAAVISKLNLVNK 500

BLAST of MS026484 vs. NCBI nr
Match: KAG7033713.1 (Serine/threonine-protein kinase STN8, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 853.2 bits (2203), Expect = 1.1e-243
Identity = 437/502 (87.05%), Postives = 462/502 (92.03%), Query Frame = 0

Query: 1   MASLLSPLSISLQITPQTLFFSPLK----SHVSLFAGTFRTK--------AFFGDISDRL 60
           MASL+ P +ISLQ T QT  FSPLK    + + LFAGTFR K        +FFGDISD L
Sbjct: 1   MASLIFPFAISLQQTHQTR-FSPLKPILPTEIPLFAGTFRPKPPAGKCRASFFGDISDDL 60

Query: 61  LDNLDGPLRPVDQLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGA 120
           L+NLDGPLR VDQLPVLQSGY+QFQRFSGELSDLQKWGFIVFAG +WVYLTARPGVLFGA
Sbjct: 61  LNNLDGPLR-VDQLPVLQSGYVQFQRFSGELSDLQKWGFIVFAGFIWVYLTARPGVLFGA 120

Query: 121 IDAYLLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRG 180
           ID YLLAPLQLGL SL GRR+LKSSDFVIG KLGEGSFGVVYSGAFLPKNVKIE R S+ 
Sbjct: 121 IDTYLLAPLQLGLYSLIGRRNLKSSDFVIGSKLGEGSFGVVYSGAFLPKNVKIENRTSKV 180

Query: 181 LEVDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFS 240
           LE+DGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRE+RQFS
Sbjct: 181 LELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRENRQFS 240

Query: 241 AGGKWLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIK 300
           AGGKWLVWKFEGDQTL DYMKDRSFP+NLE LMF RVL+G+NS+E+NALIIKQILRQII 
Sbjct: 241 AGGKWLVWKFEGDQTLGDYMKDRSFPLNLETLMFRRVLQGMNSIERNALIIKQILRQIIT 300

Query: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE 360
           SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE
Sbjct: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE 360

Query: 361 LYVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFN 420
           LYVMPEETPSPPPAPIAAL SPI+WQLNSPDLFDMYSAGIVL+QMAVP+LRSSAGLKNFN
Sbjct: 361 LYVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPSLRSSAGLKNFN 420

Query: 421 LELKNYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRH 480
           +E+KNYGYDLN+WRE+TRTKPDLTILDLDS RGWDLATKLVSERG LRRGRLSA+AALRH
Sbjct: 421 MEIKNYGYDLNEWRERTRTKPDLTILDLDSRRGWDLATKLVSERGSLRRGRLSASAALRH 480

Query: 481 PYFLLGGDQAAAVISKLNLVKK 491
           PYFLLG DQAAAVISKLNLV K
Sbjct: 481 PYFLLGSDQAAAVISKLNLVNK 500

BLAST of MS026484 vs. ExPASy Swiss-Prot
Match: Q9LZV4 (Serine/threonine-protein kinase STN8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=STN8 PE=2 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 1.5e-190
Identity = 332/440 (75.45%), Postives = 377/440 (85.68%), Query Frame = 0

Query: 58  PVDQLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGAIDAYLLAPL 117
           PVD L  L   +L FQ      SD QKWGF V AG++W YLTARPGVL GAIDAYLLAPL
Sbjct: 58  PVDSLSHLPP-FLDFQNSLATFSDTQKWGFFVSAGIVWFYLTARPGVLIGAIDAYLLAPL 117

Query: 118 QLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKN------VKIEERASRGLEV 177
           QLGLD+L GRR LK SDF++  KLGEGSFGVVY+G  LPKN      V++ +  ++ ++ 
Sbjct: 118 QLGLDTLIGRR-LKRSDFLVTEKLGEGSFGVVYAGVLLPKNSTLVDDVRVSKARAKAMDF 177

Query: 178 DG--KDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSA 237
            G  K +VILKK+KVGV+GAE+FGEYEEWFNYRLSRAAP+TCAEFLGSFVAD+ +  F+ 
Sbjct: 178 TGEFKQRVILKKVKVGVRGAEEFGEYEEWFNYRLSRAAPDTCAEFLGSFVADKTNTMFTK 237

Query: 238 GGKWLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKS 297
           GGKWLVW+FEGD+ LADYMKDRSFP NLE++MFGRVL+GV SV++ ALIIKQI+RQII S
Sbjct: 238 GGKWLVWRFEGDRDLADYMKDRSFPSNLESIMFGRVLQGVESVKRRALIIKQIMRQIITS 297

Query: 298 LKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 357
           L+KIH TGIVHRDVKPANLVVTKKGQIK+IDFGAA DLRIGKNY+P   LLDPDYCPPEL
Sbjct: 298 LRKIHGTGIVHRDVKPANLVVTKKGQIKLIDFGAAADLRIGKNYIPERTLLDPDYCPPEL 357

Query: 358 YVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFNL 417
           YV+PEETPSPPP PIAAL SPI+WQLNSPDLFDMYSAGIVLLQMAVP LRS+AGLKNFNL
Sbjct: 358 YVLPEETPSPPPEPIAALLSPILWQLNSPDLFDMYSAGIVLLQMAVPTLRSTAGLKNFNL 417

Query: 418 ELKNYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRHP 477
           E+K+  YDLN+WRE+TRT+PDL+ILDLDSGRGWDL TKL+SERG LRRGRLSAAAALRHP
Sbjct: 418 EIKSVEYDLNRWRERTRTRPDLSILDLDSGRGWDLVTKLISERGSLRRGRLSAAAALRHP 477

Query: 478 YFLLGGDQAAAVISKLNLVK 490
           YFLLGGDQAAAV+SKL+  K
Sbjct: 478 YFLLGGDQAAAVLSKLSFSK 495

BLAST of MS026484 vs. ExPASy Swiss-Prot
Match: Q9S713 (Serine/threonine-protein kinase STN7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=STN7 PE=1 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 3.7e-77
Identity = 167/398 (41.96%), Postives = 239/398 (60.05%), Query Frame = 0

Query: 97  YLTARPGVLFGAIDAYLLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLP 156
           YL A PGV  G  D ++LA ++        R + +  DFV+G KLGEGSFGVVY      
Sbjct: 101 YLWATPGVAPGFFDMFVLAFVE-----RLFRPTFRKDDFVVGKKLGEGSFGVVY------ 160

Query: 157 KNVKIEERASRGLEVDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLG 216
             V + ++ S     + + + +LKK       A ++G  E W N R+ RA   +CA+F+ 
Sbjct: 161 -KVSLSKKRS-----NEEGEYVLKK-------ATEYGAVEIWMNERVRRACGNSCADFVY 220

Query: 217 SFVADRESRQFSAGGKWLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNA 276
            F+ D+ S++      WL+WK+EG+ TLA  M+ + FP N+E ++ G+V      +E+  
Sbjct: 221 GFL-DKSSKK--GPEYWLLWKYEGESTLAGLMQSKEFPYNVETIILGKVQDLPKGLEREN 280

Query: 277 LIIKQILRQIIKSLKKIHDTGIVHRDVKPANLVVTKKGQ-IKIIDFGAATDLRIGKNYVP 336
            II+ I+RQ++ +L  +H TGI+HRDVKP N++ ++  +  KIID GAA DLR+G NY+P
Sbjct: 281 KIIQTIMRQLLFALDGLHSTGIIHRDVKPQNIIFSEGSRSFKIIDLGAAADLRVGINYIP 340

Query: 337 NLALLDPDYCPPELYVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAV 396
              LLDP Y  PE Y+M  +TPS P AP+AA  SP++WQ+N PD FD+YS G++ LQMA 
Sbjct: 341 KEFLLDPRYAAPEQYIMSTQTPSAPSAPVAAALSPVLWQMNLPDRFDIYSIGLIFLQMAF 400

Query: 397 PNLRSSAGLKNFNLELKNYGYDLNKWRE--KTRTKPDL----TILDLDSGRGWDLATKLV 456
           P+LRS + L  FN +LK   YDL  WR+  + R   DL     ++DLD G GW+L T +V
Sbjct: 401 PSLRSDSNLIQFNRQLKRCDYDLTAWRKLVEPRASADLRRGFELVDLDGGIGWELLTSMV 460

Query: 457 SERGFLRRGRLSAAAALRHPYFLLGGDQAAAVISKLNL 488
               +  R R+SA AAL HPYF   G  A +V+  L +
Sbjct: 461 R---YKARQRISAKAALAHPYFDRQGLLALSVMQNLRM 468

BLAST of MS026484 vs. ExPASy Swiss-Prot
Match: Q84V18 (Serine/threonine-protein kinase stt7, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN=STT7 PE=1 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.2e-72
Identity = 166/420 (39.52%), Postives = 225/420 (53.57%), Query Frame = 0

Query: 97  YLTARPGVLFGAIDAYLLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLP 156
           YL   PGVL GAID Y+ APL+         +++  +D V+G +LG G FG V+ G    
Sbjct: 106 YLFLPPGVLPGAIDYYIRAPLK-----RKQTKAIDKNDIVLGKRLGTGGFGTVFKG---- 165

Query: 157 KNVKIEERASRGLEVDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLG 216
                E +   G+    K  +I+KK       A++FGE E W N R+SR A    AEF+ 
Sbjct: 166 -----ELKEEGGV----KTSIIIKK-------AKEFGEAEVWMNERMSRVAGHHVAEFVT 225

Query: 217 SFVADRESRQFSAGGK----------------WLVWKFEGDQTLADYMKDRSFPVNLEAL 276
           +F         +A GK                WLVW +EGD TL+  M+ R +P NLE L
Sbjct: 226 AFDESLNVPLPAAAGKRAAPVQPTSPLDANSIWLVWVYEGDNTLSSLMERREWPYNLEPL 285

Query: 277 MFGRVLRGVNSVEKNALIIKQILRQIIKSLKKIHDTGIVHRDVKPANLVVTKKG-QIKII 336
           +FGR LR      +  + IK+  RQ+++++   H  GIVHRD+KPAN +V+++  +IK+I
Sbjct: 286 LFGRELRAPRGPVRELVTIKEAFRQLVQAVAACHSVGIVHRDIKPANCIVSERDKKIKLI 345

Query: 337 DFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPPPAPIAALFSPIVWQLNSPD 396
           D GAA DLRIG NYVPN  LLDP Y PP+ Y+M  +TP PPP P+AA  SPI+W +  PD
Sbjct: 346 DLGAAADLRIGINYVPNEYLLDPRYAPPQQYIMSTQTPKPPPKPVAAFLSPILWTMEKPD 405

Query: 397 LFDMYSAGIVLLQMAVPNLRSSAGLKNFNLELKNYGYDLNKWREKTRTK----------- 456
            FDMYS GI LLQM   +LR+   L  FN  L+   +DL  WR +   K           
Sbjct: 406 RFDMYSCGITLLQMVFGHLRNDNALIAFNKRLQELKWDLPAWRREEEAKLPSAKGALAES 465

Query: 457 --PDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRHPYFLLGGDQAAAVISKLN 487
                  LD D G GWDL  +L++   +    R SAAA L HP+      + A++   L+
Sbjct: 466 LEAGFEALDADGGAGWDLLMRLLA---YKPTDRPSAAAVLAHPWLTSAPGRTASLQHSLS 497

BLAST of MS026484 vs. ExPASy Swiss-Prot
Match: A1A5Q6 (Sperm motility kinase OS=Rattus norvegicus OX=10116 GN=Smok PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.2e-08
Identity = 46/172 (26.74%), Postives = 74/172 (43.02%), Query Frame = 0

Query: 281 QILRQIIKSLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALL 340
           ++ RQ++ +L+  H  GI+HRD+KP N+++  KG +K+ DFG +T + +G+        L
Sbjct: 121 RLFRQMVLALQYCHQRGIIHRDIKPENILLDHKGNVKLSDFGLSTKIVMGQKLTTLCGTL 180

Query: 341 DPDYCPPELYVMPEETPSPPPAPIAALFSPIVWQLNSPD--LFDMYSAGIVLLQMAVPNL 400
              YC PEL+                        LN  D    D++S G+VL  MA   L
Sbjct: 181 --PYCAPELF-----------------------NLNGYDGQAIDVWSLGVVLYYMATGCL 240

Query: 401 RSSAGLKNFNLELKNYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVS 451
                 + F  +         ++    R  PDL          WD+  KL++
Sbjct: 241 ----PFQGFTYQAIKQKILSGRYSVNFRLSPDL----------WDVIAKLLT 253

BLAST of MS026484 vs. ExPASy Swiss-Prot
Match: Q61RA2 (Serine/threonine-protein kinase chk-1 OS=Caenorhabditis briggsae OX=6238 GN=chk-1 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.8e-08
Identity = 49/144 (34.03%), Postives = 67/144 (46.53%), Query Frame = 0

Query: 284 RQIIKSLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLR-IGKNYVPNLALLDP 343
           RQ+I+ LK IHD  IVHRD+KP NL++T    +KI DFG AT  R  GK  + +L+    
Sbjct: 132 RQLIEGLKYIHDCDIVHRDIKPENLLLTTSHVLKISDFGMATLYRNEGKERLLDLSCGTI 191

Query: 344 DYCPPELYVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNL---R 403
            Y  PE+    +    P                      D++S+GIVL+ M    L   R
Sbjct: 192 PYAAPEVCAGGKYRGPP---------------------IDVWSSGIVLIAMLTGELPWDR 251

Query: 404 SSAGLKNFNLELKNYGYDLNKWRE 424
           +S     +   L N   D N WR+
Sbjct: 252 ASDSSYAYLQWLGNNNLDENPWRK 254

BLAST of MS026484 vs. ExPASy TrEMBL
Match: A0A6J1CSB0 (serine/threonine-protein kinase STN8, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111013876 PE=4 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 3.0e-279
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MASLLSPLSISLQITPQTLFFSPLKSHVSLFAGTFRTKAFFGDISDRLLDNLDGPLRPVD 60
           MASLLSPLSISLQITPQTLFFSPLKSHVSLFAGTFRTKAFFGDISDRLLDNLDGPLRPVD
Sbjct: 1   MASLLSPLSISLQITPQTLFFSPLKSHVSLFAGTFRTKAFFGDISDRLLDNLDGPLRPVD 60

Query: 61  QLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGAIDAYLLAPLQLG 120
           QLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGAIDAYLLAPLQLG
Sbjct: 61  QLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGAIDAYLLAPLQLG 120

Query: 121 LDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRGLEVDGKDKVILK 180
           LDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRGLEVDGKDKVILK
Sbjct: 121 LDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRGLEVDGKDKVILK 180

Query: 181 KIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSAGGKWLVWKFEG 240
           KIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSAGGKWLVWKFEG
Sbjct: 181 KIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSAGGKWLVWKFEG 240

Query: 241 DQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKSLKKIHDTGIVH 300
           DQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKSLKKIHDTGIVH
Sbjct: 241 DQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKSLKKIHDTGIVH 300

Query: 301 RDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPP 360
           RDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPP
Sbjct: 301 RDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVMPEETPSPP 360

Query: 361 PAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFNLELKNYGYDLNK 420
           PAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFNLELKNYGYDLNK
Sbjct: 361 PAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFNLELKNYGYDLNK 420

Query: 421 WREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRHPYFLLGGDQAAA 480
           WREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRHPYFLLGGDQAAA
Sbjct: 421 WREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRHPYFLLGGDQAAA 480

Query: 481 VISKLNLVKK 491
           VISKLNLVKK
Sbjct: 481 VISKLNLVKK 490

BLAST of MS026484 vs. ExPASy TrEMBL
Match: A0A6J1GDZ5 (serine/threonine-protein kinase STN8, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111453317 PE=4 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 1.8e-244
Identity = 437/502 (87.05%), Postives = 463/502 (92.23%), Query Frame = 0

Query: 1   MASLLSPLSISLQITPQTLFFSPLK----SHVSLFAGTFRTK--------AFFGDISDRL 60
           MASL+ P +ISLQ T QT  FSPLK    + + LFAGTFR K        +FFGDISD L
Sbjct: 1   MASLIFPFAISLQQTHQTR-FSPLKPILPTEIPLFAGTFRPKPPAGKCRASFFGDISDDL 60

Query: 61  LDNLDGPLRPVDQLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGA 120
           L+NLDGPLR VDQLPVLQSGY+QFQRFSGELSDLQKWGFIVFAG +WVYLTARPGVLFGA
Sbjct: 61  LNNLDGPLR-VDQLPVLQSGYVQFQRFSGELSDLQKWGFIVFAGFIWVYLTARPGVLFGA 120

Query: 121 IDAYLLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRG 180
           ID YLLAPLQLGL SL GRR+LKSSDFVIG KLGEGSFGVVYSGAFLPKNVKIE R S+ 
Sbjct: 121 IDTYLLAPLQLGLYSLIGRRNLKSSDFVIGSKLGEGSFGVVYSGAFLPKNVKIENRTSKV 180

Query: 181 LEVDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFS 240
           LE+DGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRE+RQFS
Sbjct: 181 LELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRENRQFS 240

Query: 241 AGGKWLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIK 300
           AGGKWLVWKFEGDQTL DYMKDRSFP+NLE LMF RVL+G+NS+E+NALIIKQILRQII 
Sbjct: 241 AGGKWLVWKFEGDQTLGDYMKDRSFPLNLETLMFRRVLQGMNSIERNALIIKQILRQIIT 300

Query: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE 360
           SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNY+PNLALLDPDYCPPE
Sbjct: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYIPNLALLDPDYCPPE 360

Query: 361 LYVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFN 420
           LYVMPEETPSPPPAPIAAL SPI+WQLNSPDLFDMYSAGIVL+QMAVP+LRSSAGLKNFN
Sbjct: 361 LYVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPSLRSSAGLKNFN 420

Query: 421 LELKNYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRH 480
           +E+KNYGYDLN+WRE+TRTKPDLTILDLDS RGWDLATKLVSERG LRRGRLSA+AALRH
Sbjct: 421 MEIKNYGYDLNEWRERTRTKPDLTILDLDSRRGWDLATKLVSERGSLRRGRLSASAALRH 480

Query: 481 PYFLLGGDQAAAVISKLNLVKK 491
           PYFLLG DQAAAVISKLNLVKK
Sbjct: 481 PYFLLGSDQAAAVISKLNLVKK 500

BLAST of MS026484 vs. ExPASy TrEMBL
Match: A0A6J1IS67 (serine/threonine-protein kinase STN8, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111478215 PE=4 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.3e-244
Identity = 436/502 (86.85%), Postives = 462/502 (92.03%), Query Frame = 0

Query: 1   MASLLSPLSISLQITPQTLFFSPLK----SHVSLFAGTFRTK--------AFFGDISDRL 60
           MASL+ P +ISLQ T QT  FSPLK    + + LFAGTFR K        +FFGDISD L
Sbjct: 1   MASLIFPFAISLQQTHQTR-FSPLKPILPTEIPLFAGTFRPKPSAGKCRASFFGDISDDL 60

Query: 61  LDNLDGPLRPVDQLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGA 120
           L+NLDGPLR +DQLPVLQSGY+QFQRFSGELSDLQKWGFIVFAG +WVYLTARPGVLFGA
Sbjct: 61  LNNLDGPLR-IDQLPVLQSGYVQFQRFSGELSDLQKWGFIVFAGFIWVYLTARPGVLFGA 120

Query: 121 IDAYLLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRG 180
           ID YLLAPLQLGL SL GRR+LKSSDFVIG KLGEGSFGVVYSGAFLPKNVKIE R S+ 
Sbjct: 121 IDTYLLAPLQLGLYSLIGRRNLKSSDFVIGSKLGEGSFGVVYSGAFLPKNVKIENRTSKV 180

Query: 181 LEVDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFS 240
           LE+DGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRE+RQFS
Sbjct: 181 LELDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRENRQFS 240

Query: 241 AGGKWLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIK 300
           AGGKWLVWKFEGDQTL DYMKDRSFP+NLE LMF RVL+G+NS E+NALIIKQILRQII 
Sbjct: 241 AGGKWLVWKFEGDQTLGDYMKDRSFPLNLETLMFRRVLQGMNSTERNALIIKQILRQIIT 300

Query: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE 360
           SLKKIHDTGIVHRDVKPANLVVTKKGQ+KIIDFGAATDLRIGKNYVPNLALLDPDYCPPE
Sbjct: 301 SLKKIHDTGIVHRDVKPANLVVTKKGQVKIIDFGAATDLRIGKNYVPNLALLDPDYCPPE 360

Query: 361 LYVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFN 420
           LYVMPEETPSPPPAPIAAL SPI+WQLNSPDLFDMYSAGIVL+QMAVP+LRSSAGLKNFN
Sbjct: 361 LYVMPEETPSPPPAPIAALLSPILWQLNSPDLFDMYSAGIVLMQMAVPSLRSSAGLKNFN 420

Query: 421 LELKNYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRH 480
           +E+KNYGYDLN+WRE+TRTKPDLTILDLDS RGWDLATKLVSERG LRRGRLSA+AALRH
Sbjct: 421 MEIKNYGYDLNEWRERTRTKPDLTILDLDSRRGWDLATKLVSERGSLRRGRLSASAALRH 480

Query: 481 PYFLLGGDQAAAVISKLNLVKK 491
           PYFLLG DQAAAVISKLNLVKK
Sbjct: 481 PYFLLGSDQAAAVISKLNLVKK 500

BLAST of MS026484 vs. ExPASy TrEMBL
Match: A0A5D3CEV2 (Serine/threonine-protein kinase STN8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00410 PE=4 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.6e-235
Identity = 423/498 (84.94%), Postives = 449/498 (90.16%), Query Frame = 0

Query: 1   MASLLSPLSISLQITPQTLFFSPLKSHVSLFAGTFRTK--------AFFGDISDRLLDNL 60
           MASL+ P +ISLQ T  +     L++ V L  GTF  K        +FFGDISD LLDNL
Sbjct: 1   MASLIFPFAISLQQTRLSPLKPTLQTQVPLLPGTFTPKPLAGKCRASFFGDISDNLLDNL 60

Query: 61  DGPLRPVDQLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGAIDAY 120
           DGPL+ VDQLPV QSGY+QFQRFSG LSDLQKWGF+VFAG +WVYLTARPGVL GAIDAY
Sbjct: 61  DGPLK-VDQLPVFQSGYVQFQRFSGGLSDLQKWGFVVFAGFIWVYLTARPGVLVGAIDAY 120

Query: 121 LLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRGLEVD 180
           LLAPLQLGLDSL GRRSLKSSDFVIGGKLGEGSFGVVY+GAFLPKNVK EER SR L  D
Sbjct: 121 LLAPLQLGLDSLLGRRSLKSSDFVIGGKLGEGSFGVVYAGAFLPKNVKNEERTSRAL--D 180

Query: 181 GKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSAGGK 240
           GK+KVILKKIK+GVKGAE+ GEYE WFNYRLSRAAPETCA+FLGSFVADR SRQFSAGGK
Sbjct: 181 GKEKVILKKIKLGVKGAEELGEYEVWFNYRLSRAAPETCADFLGSFVADRASRQFSAGGK 240

Query: 241 WLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKSLKK 300
           WLVWKFEGDQTL DYMKDRSFPVNLE++MFGRVLRG+NSVE+NALIIKQILRQII SLKK
Sbjct: 241 WLVWKFEGDQTLGDYMKDRSFPVNLESVMFGRVLRGMNSVERNALIIKQILRQIITSLKK 300

Query: 301 IHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVM 360
           IHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPN ALLDPDYCPPELYVM
Sbjct: 301 IHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNRALLDPDYCPPELYVM 360

Query: 361 PEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFNLELK 420
           PEETPSPPPAPIAAL SPI+WQLN+PDLFDMYSAGIVL+QMAVPNLRSSAGLKNFN+E+K
Sbjct: 361 PEETPSPPPAPIAALLSPILWQLNNPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNMEIK 420

Query: 421 NYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRHPYFL 480
           NYGYDLNKWREKTRTKPDLTILDLDSGRGWDL TKLV+E    RRGRLSA+AALRHPYFL
Sbjct: 421 NYGYDLNKWREKTRTKPDLTILDLDSGRGWDLVTKLVTE----RRGRLSASAALRHPYFL 480

Query: 481 LGGDQAAAVISKLNLVKK 491
           LG DQAAAVISKLNLVKK
Sbjct: 481 LGSDQAAAVISKLNLVKK 491

BLAST of MS026484 vs. ExPASy TrEMBL
Match: A0A1S3CIV9 (serine/threonine-protein kinase STN8, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501471 PE=4 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.6e-235
Identity = 423/498 (84.94%), Postives = 449/498 (90.16%), Query Frame = 0

Query: 1   MASLLSPLSISLQITPQTLFFSPLKSHVSLFAGTFRTK--------AFFGDISDRLLDNL 60
           MASL+ P +ISLQ T  +     L++ V L  GTF  K        +FFGDISD LLDNL
Sbjct: 1   MASLIFPFAISLQQTRLSPLKPTLQTQVPLLPGTFTPKPLAGKCRASFFGDISDNLLDNL 60

Query: 61  DGPLRPVDQLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGAIDAY 120
           DGPL+ VDQLPV QSGY+QFQRFSG LSDLQKWGF+VFAG +WVYLTARPGVL GAIDAY
Sbjct: 61  DGPLK-VDQLPVFQSGYVQFQRFSGGLSDLQKWGFVVFAGFIWVYLTARPGVLVGAIDAY 120

Query: 121 LLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRGLEVD 180
           LLAPLQLGLDSL GRRSLKSSDFVIGGKLGEGSFGVVY+GAFLPKNVK EER SR L  D
Sbjct: 121 LLAPLQLGLDSLLGRRSLKSSDFVIGGKLGEGSFGVVYAGAFLPKNVKNEERTSRAL--D 180

Query: 181 GKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSAGGK 240
           GK+KVILKKIK+GVKGAE+ GEYE WFNYRLSRAAPETCA+FLGSFVADR SRQFSAGGK
Sbjct: 181 GKEKVILKKIKLGVKGAEELGEYEVWFNYRLSRAAPETCADFLGSFVADRASRQFSAGGK 240

Query: 241 WLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKSLKK 300
           WLVWKFEGDQTL DYMKDRSFPVNLE++MFGRVLRG+NSVE+NALIIKQILRQII SLKK
Sbjct: 241 WLVWKFEGDQTLGDYMKDRSFPVNLESVMFGRVLRGMNSVERNALIIKQILRQIITSLKK 300

Query: 301 IHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPELYVM 360
           IHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPN ALLDPDYCPPELYVM
Sbjct: 301 IHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNRALLDPDYCPPELYVM 360

Query: 361 PEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFNLELK 420
           PEETPSPPPAPIAAL SPI+WQLN+PDLFDMYSAGIVL+QMAVPNLRSSAGLKNFN+E+K
Sbjct: 361 PEETPSPPPAPIAALLSPILWQLNNPDLFDMYSAGIVLMQMAVPNLRSSAGLKNFNMEIK 420

Query: 421 NYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRHPYFL 480
           NYGYDLNKWREKTRTKPDLTILDLDSGRGWDL TKLV+E    RRGRLSA+AALRHPYFL
Sbjct: 421 NYGYDLNKWREKTRTKPDLTILDLDSGRGWDLVTKLVTE----RRGRLSASAALRHPYFL 480

Query: 481 LGGDQAAAVISKLNLVKK 491
           LG DQAAAVISKLNLVKK
Sbjct: 481 LGSDQAAAVISKLNLVKK 491

BLAST of MS026484 vs. TAIR 10
Match: AT5G01920.1 (Protein kinase superfamily protein )

HSP 1 Score: 667.2 bits (1720), Expect = 1.0e-191
Identity = 332/440 (75.45%), Postives = 377/440 (85.68%), Query Frame = 0

Query: 58  PVDQLPVLQSGYLQFQRFSGELSDLQKWGFIVFAGVLWVYLTARPGVLFGAIDAYLLAPL 117
           PVD L  L   +L FQ      SD QKWGF V AG++W YLTARPGVL GAIDAYLLAPL
Sbjct: 58  PVDSLSHLPP-FLDFQNSLATFSDTQKWGFFVSAGIVWFYLTARPGVLIGAIDAYLLAPL 117

Query: 118 QLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLPKN------VKIEERASRGLEV 177
           QLGLD+L GRR LK SDF++  KLGEGSFGVVY+G  LPKN      V++ +  ++ ++ 
Sbjct: 118 QLGLDTLIGRR-LKRSDFLVTEKLGEGSFGVVYAGVLLPKNSTLVDDVRVSKARAKAMDF 177

Query: 178 DG--KDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSA 237
            G  K +VILKK+KVGV+GAE+FGEYEEWFNYRLSRAAP+TCAEFLGSFVAD+ +  F+ 
Sbjct: 178 TGEFKQRVILKKVKVGVRGAEEFGEYEEWFNYRLSRAAPDTCAEFLGSFVADKTNTMFTK 237

Query: 238 GGKWLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKS 297
           GGKWLVW+FEGD+ LADYMKDRSFP NLE++MFGRVL+GV SV++ ALIIKQI+RQII S
Sbjct: 238 GGKWLVWRFEGDRDLADYMKDRSFPSNLESIMFGRVLQGVESVKRRALIIKQIMRQIITS 297

Query: 298 LKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLDPDYCPPEL 357
           L+KIH TGIVHRDVKPANLVVTKKGQIK+IDFGAA DLRIGKNY+P   LLDPDYCPPEL
Sbjct: 298 LRKIHGTGIVHRDVKPANLVVTKKGQIKLIDFGAAADLRIGKNYIPERTLLDPDYCPPEL 357

Query: 358 YVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSSAGLKNFNL 417
           YV+PEETPSPPP PIAAL SPI+WQLNSPDLFDMYSAGIVLLQMAVP LRS+AGLKNFNL
Sbjct: 358 YVLPEETPSPPPEPIAALLSPILWQLNSPDLFDMYSAGIVLLQMAVPTLRSTAGLKNFNL 417

Query: 418 ELKNYGYDLNKWREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRGRLSAAAALRHP 477
           E+K+  YDLN+WRE+TRT+PDL+ILDLDSGRGWDL TKL+SERG LRRGRLSAAAALRHP
Sbjct: 418 EIKSVEYDLNRWRERTRTRPDLSILDLDSGRGWDLVTKLISERGSLRRGRLSAAAALRHP 477

Query: 478 YFLLGGDQAAAVISKLNLVK 490
           YFLLGGDQAAAV+SKL+  K
Sbjct: 478 YFLLGGDQAAAVLSKLSFSK 495

BLAST of MS026484 vs. TAIR 10
Match: AT1G68830.1 (STT7 homolog STN7 )

HSP 1 Score: 290.4 bits (742), Expect = 2.7e-78
Identity = 167/398 (41.96%), Postives = 239/398 (60.05%), Query Frame = 0

Query: 97  YLTARPGVLFGAIDAYLLAPLQLGLDSLTGRRSLKSSDFVIGGKLGEGSFGVVYSGAFLP 156
           YL A PGV  G  D ++LA ++        R + +  DFV+G KLGEGSFGVVY      
Sbjct: 101 YLWATPGVAPGFFDMFVLAFVE-----RLFRPTFRKDDFVVGKKLGEGSFGVVY------ 160

Query: 157 KNVKIEERASRGLEVDGKDKVILKKIKVGVKGAEKFGEYEEWFNYRLSRAAPETCAEFLG 216
             V + ++ S     + + + +LKK       A ++G  E W N R+ RA   +CA+F+ 
Sbjct: 161 -KVSLSKKRS-----NEEGEYVLKK-------ATEYGAVEIWMNERVRRACGNSCADFVY 220

Query: 217 SFVADRESRQFSAGGKWLVWKFEGDQTLADYMKDRSFPVNLEALMFGRVLRGVNSVEKNA 276
            F+ D+ S++      WL+WK+EG+ TLA  M+ + FP N+E ++ G+V      +E+  
Sbjct: 221 GFL-DKSSKK--GPEYWLLWKYEGESTLAGLMQSKEFPYNVETIILGKVQDLPKGLEREN 280

Query: 277 LIIKQILRQIIKSLKKIHDTGIVHRDVKPANLVVTKKGQ-IKIIDFGAATDLRIGKNYVP 336
            II+ I+RQ++ +L  +H TGI+HRDVKP N++ ++  +  KIID GAA DLR+G NY+P
Sbjct: 281 KIIQTIMRQLLFALDGLHSTGIIHRDVKPQNIIFSEGSRSFKIIDLGAAADLRVGINYIP 340

Query: 337 NLALLDPDYCPPELYVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAV 396
              LLDP Y  PE Y+M  +TPS P AP+AA  SP++WQ+N PD FD+YS G++ LQMA 
Sbjct: 341 KEFLLDPRYAAPEQYIMSTQTPSAPSAPVAAALSPVLWQMNLPDRFDIYSIGLIFLQMAF 400

Query: 397 PNLRSSAGLKNFNLELKNYGYDLNKWRE--KTRTKPDL----TILDLDSGRGWDLATKLV 456
           P+LRS + L  FN +LK   YDL  WR+  + R   DL     ++DLD G GW+L T +V
Sbjct: 401 PSLRSDSNLIQFNRQLKRCDYDLTAWRKLVEPRASADLRRGFELVDLDGGIGWELLTSMV 460

Query: 457 SERGFLRRGRLSAAAALRHPYFLLGGDQAAAVISKLNL 488
               +  R R+SA AAL HPYF   G  A +V+  L +
Sbjct: 461 R---YKARQRISAKAALAHPYFDRQGLLALSVMQNLRM 468

BLAST of MS026484 vs. TAIR 10
Match: AT3G07980.1 (mitogen-activated protein kinase kinase kinase 6 )

HSP 1 Score: 59.3 bits (142), Expect = 1.0e-08
Identity = 43/193 (22.28%), Postives = 87/193 (45.08%), Query Frame = 0

Query: 135 FVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRGLEVDGKDKVILKKIKVGVKGAEKFGE 194
           +++G ++G+G++G VY                 GL+++  D V +K++ +   G E    
Sbjct: 20  YMLGDEIGKGAYGRVYI----------------GLDLENGDFVAIKQVSLENIGQEDLNT 79

Query: 195 YEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSAGGKWLVWKFEGDQTLADYMKDRSFP 254
             +  +  L     +   ++LGS                ++ ++  + +LA+ +K   F 
Sbjct: 80  IMQEIDL-LKNLNHKNIVKYLGSLKTKTHLH--------IILEYVENGSLANIIKPNKFG 139

Query: 255 VNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKSLKKIHDTGIVHRDVKPANLVVTKKG 314
              E+L                  +   + Q+++ L  +H+ G++HRD+K AN++ TK+G
Sbjct: 140 PFPESL------------------VTVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKEG 169

Query: 315 QIKIIDFGAATDL 328
            +K+ DFG AT L
Sbjct: 200 LVKLADFGVATKL 169

BLAST of MS026484 vs. TAIR 10
Match: AT3G13530.1 (mitogen-activated protein kinase kinase kinase 7 )

HSP 1 Score: 56.6 bits (135), Expect = 6.5e-08
Identity = 42/193 (21.76%), Postives = 87/193 (45.08%), Query Frame = 0

Query: 135 FVIGGKLGEGSFGVVYSGAFLPKNVKIEERASRGLEVDGKDKVILKKIKVGVKGAEKFGE 194
           +++G ++G+G++G VY                +GL+++  D V +K++ +     E    
Sbjct: 20  YMLGDEIGKGAYGRVY----------------KGLDLENGDFVAIKQVSLENIVQEDLNT 79

Query: 195 YEEWFNYRLSRAAPETCAEFLGSFVADRESRQFSAGGKWLVWKFEGDQTLADYMKDRSFP 254
             +  +  L     +   ++LGS                ++ ++  + +LA+ +K   F 
Sbjct: 80  IMQEIDL-LKNLNHKNIVKYLGSSKTKTHLH--------IILEYVENGSLANIIKPNKFG 139

Query: 255 VNLEALMFGRVLRGVNSVEKNALIIKQILRQIIKSLKKIHDTGIVHRDVKPANLVVTKKG 314
              E+L                  +   + Q+++ L  +H+ G++HRD+K AN++ TK+G
Sbjct: 140 PFPESL------------------VAVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKEG 169

Query: 315 QIKIIDFGAATDL 328
            +K+ DFG AT L
Sbjct: 200 LVKLADFGVATKL 169

BLAST of MS026484 vs. TAIR 10
Match: AT1G12680.1 (phosphoenolpyruvate carboxylase-related kinase 2 )

HSP 1 Score: 56.2 bits (134), Expect = 8.4e-08
Identity = 52/199 (26.13%), Postives = 81/199 (40.70%), Query Frame = 0

Query: 282 ILRQIIKSLKKIHDTGIVHRDVKPANLVVTKKGQIKIIDFGAATDLRIGKNYVPNLALLD 341
           I + ++  +   H+ G+VHRD+KP N+++T  G+I++ DFG A  +RI K    +     
Sbjct: 204 IFKDLMLVINYCHEMGVVHRDIKPENILLTAAGKIQLADFGLA--MRIAKGQTLSGLAGS 263

Query: 342 PDYCPPELYVMPEETPSPPPAPIAALFSPIVWQLNSPDLFDMYSAGIVLLQMAVPNLRSS 401
           P Y  PE                       V   N  +  D++SAG++L  +    L   
Sbjct: 264 PAYVAPE-----------------------VLSENYSEKVDVWSAGVLLYALLSGVLPFK 323

Query: 402 AGLKNFNLE-LKNYGYDLNK--WREKTRTKPDLTILDLDSGRGWDLATKLVSERGFLRRG 461
               +   E +KN   D N   W   ++   DL            LA  L  E       
Sbjct: 324 GDSLDAIFEAIKNVKLDFNTGVWESVSKPARDL------------LARMLTREES----A 361

Query: 462 RLSAAAALRHPYFLLGGDQ 478
           R++A   LRHP+ L   D+
Sbjct: 384 RITADEVLRHPWILFYTDR 361

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144101.16.2e-279100.00serine/threonine-protein kinase STN8, chloroplastic [Momordica charantia][more]
XP_022950141.13.8e-24487.05serine/threonine-protein kinase STN8, chloroplastic [Cucurbita moschata][more]
XP_023545193.13.8e-24487.05serine/threonine-protein kinase STN8, chloroplastic [Cucurbita pepo subsp. pepo][more]
KAG6603530.11.1e-24387.05Serine/threonine-protein kinase STN8, chloroplastic, partial [Cucurbita argyrosp... [more]
KAG7033713.11.1e-24387.05Serine/threonine-protein kinase STN8, chloroplastic [Cucurbita argyrosperma subs... [more]
Match NameE-valueIdentityDescription
Q9LZV41.5e-19075.45Serine/threonine-protein kinase STN8, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q9S7133.7e-7741.96Serine/threonine-protein kinase STN7, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q84V181.2e-7239.52Serine/threonine-protein kinase stt7, chloroplastic OS=Chlamydomonas reinhardtii... [more]
A1A5Q62.2e-0826.74Sperm motility kinase OS=Rattus norvegicus OX=10116 GN=Smok PE=2 SV=1[more]
Q61RA22.8e-0834.03Serine/threonine-protein kinase chk-1 OS=Caenorhabditis briggsae OX=6238 GN=chk-... [more]
Match NameE-valueIdentityDescription
A0A6J1CSB03.0e-279100.00serine/threonine-protein kinase STN8, chloroplastic OS=Momordica charantia OX=36... [more]
A0A6J1GDZ51.8e-24487.05serine/threonine-protein kinase STN8, chloroplastic OS=Cucurbita moschata OX=366... [more]
A0A6J1IS675.3e-24486.85serine/threonine-protein kinase STN8, chloroplastic OS=Cucurbita maxima OX=3661 ... [more]
A0A5D3CEV21.6e-23584.94Serine/threonine-protein kinase STN8 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3CIV91.6e-23584.94serine/threonine-protein kinase STN8, chloroplastic OS=Cucumis melo OX=3656 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G01920.11.0e-19175.45Protein kinase superfamily protein [more]
AT1G68830.12.7e-7841.96STT7 homolog STN7 [more]
AT3G07980.11.0e-0822.28mitogen-activated protein kinase kinase kinase 6 [more]
AT3G13530.16.5e-0821.76mitogen-activated protein kinase kinase kinase 7 [more]
AT1G12680.18.4e-0826.13phosphoenolpyruvate carboxylase-related kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 135..471
e-value: 1.4E-10
score: 17.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 278..349
e-value: 7.5E-18
score: 64.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 135..471
score: 24.075161
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 120..238
e-value: 5.9E-22
score: 79.8
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 270..473
e-value: 3.6E-44
score: 152.9
NoneNo IPR availablePANTHERPTHR46699:SF1SERINE/THREONINE-PROTEIN KINASE STN8, CHLOROPLASTICcoord: 1..489
IPR044803Serine/threonine-protein kinase STT7-likePANTHERPTHR46699SERINE/THREONINE-PROTEIN KINASE STN8, CHLOROPLASTIC-RELATEDcoord: 1..489
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 298..310
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 131..472

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS026484.1MS026484.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0009579 thylakoid
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity