MS026429 (gene) Bitter gourd (TR) v1

Overview


Name	MS026429
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	cysteine proteinase inhibitor 1-like
Location	scaffold402: 1416425 .. 1416841 (+)
RNA-Seq Expression	MS026429
Synteny	MS026429

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAAGTGTGTTTTGTGGTTGGCTTCTGTCCTCCTGCTGGTGGCGGCGGCAGGTTTGGCAAATGGTGCTGCTGAAGACCAATTATCTGAGCCCGGCGGACGTTACCCAATAGATGACATAGATGACCCAAAATACCAAACCCTGGCCAAACTGGTTGTGGAAATCCACAATTGGCAAGCCAACTCTCACCTGCACTTCCAAGAAGCGGTGTGTGGATGGTCACAAATCACCAAAGGAACACTCTATCAGTTTTGCCTAAGGCTCATTGATAAAGACAATGACAATGCAAAGGTTCGCTACATAACCCAGGTGGTTGTAGACCCGACCGACAACATCTCCATCGTCTTCTTCAACCTCGTCCCCAATGACCTTGAGTGTTGCTATTTCTGGGACCAGAAGCTAAAGCTTAATTAA

mRNA sequence

ATGGGAAAGTGTGTTTTGTGGTTGGCTTCTGTCCTCCTGCTGGTGGCGGCGGCAGGTTTGGCAAATGGTGCTGCTGAAGACCAATTATCTGAGCCCGGCGGACGTTACCCAATAGATGACATAGATGACCCAAAATACCAAACCCTGGCCAAACTGGTTGTGGAAATCCACAATTGGCAAGCCAACTCTCACCTGCACTTCCAAGAAGCGGTGTGTGGATGGTCACAAATCACCAAAGGAACACTCTATCAGTTTTGCCTAAGGCTCATTGATAAAGACAATGACAATGCAAAGGTTCGCTACATAACCCAGGTGGTTGTAGACCCGACCGACAACATCTCCATCGTCTTCTTCAACCTCGTCCCCAATGACCTTGAGTGTTGCTATTTCTGGGACCAGAAGCTAAAGCTTAATTAA

Coding sequence (CDS)

ATGGGAAAGTGTGTTTTGTGGTTGGCTTCTGTCCTCCTGCTGGTGGCGGCGGCAGGTTTGGCAAATGGTGCTGCTGAAGACCAATTATCTGAGCCCGGCGGACGTTACCCAATAGATGACATAGATGACCCAAAATACCAAACCCTGGCCAAACTGGTTGTGGAAATCCACAATTGGCAAGCCAACTCTCACCTGCACTTCCAAGAAGCGGTGTGTGGATGGTCACAAATCACCAAAGGAACACTCTATCAGTTTTGCCTAAGGCTCATTGATAAAGACAATGACAATGCAAAGGTTCGCTACATAACCCAGGTGGTTGTAGACCCGACCGACAACATCTCCATCGTCTTCTTCAACCTCGTCCCCAATGACCTTGAGTGTTGCTATTTCTGGGACCAGAAGCTAAAGCTTAATTAA

Protein sequence

MGKCVLWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQANSHLHFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVDPTDNISIVFFNLVPNDLECCYFWDQKLKLN

Homology

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BLAST of MS026429 vs. NCBI nr
Match: XP_022142678.1 (cystatin-1-like [Momordica charantia])

HSP 1 Score: 161.8 bits (408), Expect = 4.3e-36
Identity = 82/129 (63.57%), Postives = 95/129 (73.64%), Query Frame = 0


Query: 1   MGKCVLWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQ 60
           MGK VLWLAS+LLLV AA       E+QLSEPGG  P+ DI DPKYQ+LAK+ VEIHNWQ
Sbjct: 1   MGKSVLWLASLLLLVVAA----SGVEEQLSEPGGLKPV-DISDPKYQSLAKITVEIHNWQ 60

Query: 61  ANSHLHFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVDPTDNISIVFFNL 120
             S+L F E VCGWS+I +G  YQFCLRLIDKDN+NA   Y   V +DP+ NI I  F+L
Sbjct: 61  VGSNLQFLELVCGWSRIVRGINYQFCLRLIDKDNNNAVFHYKIDVTLDPSANIFINDFHL 120

Query: 121 VPNDLECCY 130
           VP DLECC+
Sbjct: 121 VPIDLECCF 124

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BLAST of MS026429 vs. NCBI nr
Match: XP_023520258.1 (cysteine proteinase inhibitor 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 65.1 bits (157), Expect = 5.5e-07
Identity = 43/118 (36.44%), Postives = 65/118 (55.08%), Query Frame = 0


Query: 5   VLWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQANSH 64
           VL+L + LLL   + L+  AA  +L   G   PI++IDDPK Q+L +  V  HN QA + 
Sbjct: 4   VLFLVASLLLYVLSLLSLAAATQRLDLAGSYKPIENIDDPKIQSLGEFAVNEHNKQAKTQ 63

Query: 65  LHFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVDPTDNISIVFFNLVP 123
           + FQ+ + G  QI  GT Y+  LRL   +  +++  Y T V  D  +  +++ F  VP
Sbjct: 64  IQFQKVIGGEMQIVAGTNYK--LRLTALEGTSSRT-YGTLVFTDLNNKNNLINFYKVP 118

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BLAST of MS026429 vs. NCBI nr
Match: XP_022975330.1 (cysteine proteinase inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 64.7 bits (156), Expect = 7.2e-07
Identity = 44/123 (35.77%), Postives = 66/123 (53.66%), Query Frame = 0


Query: 1   MGKCVLWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQ 60
           M K + ++AS+LL V +   +   A  +L   G   PI +ID+PK Q+L +  V  HN Q
Sbjct: 1   MAKALFFVASLLLYVLSQS-SLAVATQRLDLVGSYKPIKNIDEPKIQSLGEFAVNEHNKQ 60

Query: 61  ANSHLHFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVDPTDNISIVFFNL 120
           A + LHFQ+ + G  QI  GT Y   LRL   +  +++  Y T V  D  +  +++ F  
Sbjct: 61  AKTQLHFQKVISGEMQIVAGTNYN--LRLTALEGTDSRT-YGTLVFTDLNNKNNLINFFD 119

Query: 121 VPN 124
           VPN
Sbjct: 121 VPN 119

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BLAST of MS026429 vs. NCBI nr
Match: KAG6583736.1 (Cysteine proteinase inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 63.2 bits (152), Expect = 2.1e-06
Identity = 43/123 (34.96%), Postives = 65/123 (52.85%), Query Frame = 0


Query: 1   MGKCVLWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQ 60
           M K +  +AS+LL + +   +  AA  +L   G   PI++IDDPK Q+L +  V  HN +
Sbjct: 1   MAKALFLVASLLLYILSLS-SLAAATQRLDLVGNYKPIENIDDPKIQSLGEFAVNEHNKK 60

Query: 61  ANSHLHFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVDPTDNISIVFFNL 120
           A + LHFQ  + G  QI  GT Y   LRL   +  +++  Y T V  D  +  +++ F  
Sbjct: 61  AKTQLHFQRVISGEMQIVAGTNYN--LRLTALEGTSSRT-YGTLVFTDLNNKNNLINFFS 119

Query: 121 VPN 124
           V N
Sbjct: 121 VSN 119

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BLAST of MS026429 vs. NCBI nr
Match: XP_023520817.1 (cysteine proteinase inhibitor 1-like [Cucurbita pepo subsp. pepo] >XP_023521297.1 cysteine proteinase inhibitor 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 62.8 bits (151), Expect = 2.7e-06
Identity = 41/117 (35.04%), Postives = 61/117 (52.14%), Query Frame = 0


Query: 6   LWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQANSHL 65
           L+LA+ LLL   +  +  AA ++L   G   PI++IDDPK Q+L +  V  HN QA + L
Sbjct: 5   LFLAASLLLYVLSLSSLAAATNRLDLVGSYKPIENIDDPKIQSLGEFTVNEHNKQAKTQL 64

Query: 66  HFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVDPTDNISIVFFNLVP 123
            FQ+ + G  QI  GT Y   L  ++  +      Y T V  D  +  +++ F  VP
Sbjct: 65  QFQKVISGEMQIVAGTNYNLRLTALEGTSSGT---YGTLVFTDLNNKNNLINFYDVP 118

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BLAST of MS026429 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.4e-05
Identity = 36/103 (34.95%), Postives = 50/103 (48.54%), Query Frame = 0


Query: 6   LWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQANSHL 65
           L L   LLL  +A +  G    +L   GG  PI+ ++  + Q +A+  V  HN QAN  L
Sbjct: 6   LSLLLFLLLALSAAVVGGR---KLVAAGGWRPIESLNSAEVQDVAQFAVSEHNKQANDEL 65

Query: 66  HFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVD 109
            +Q  V G++Q+  GT Y    RL+    D A V     VV D
Sbjct: 66  QYQSVVRGYTQVVAGTNY----RLVIAAKDGAVVGNYEAVVWD 101

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BLAST of MS026429 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 3.1e-05
Identity = 35/103 (33.98%), Postives = 51/103 (49.51%), Query Frame = 0


Query: 6   LWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQANSHL 65
           L L  +LLL  +A +  G    +    GG  PI++++  + Q +A+  V  HN QAN  L
Sbjct: 6   LSLLLLLLLALSAAVVGGR---KRVAAGGWRPIENLNSAEVQDVAQFAVSEHNKQANDEL 65

Query: 66  HFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVD 109
            +Q  V G++Q+  GT Y    RL+    D A V     VV D
Sbjct: 66  QYQSVVRGYTQVVAGTNY----RLVIAAKDGAVVGNYEAVVWD 101

[Close]

BLAST of MS026429 vs. ExPASy TrEMBL
Match: A0A6J1CNV2 (cystatin-1-like OS=Momordica charantia OX=3673 GN=LOC111012734 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 2.1e-36
Identity = 82/129 (63.57%), Postives = 95/129 (73.64%), Query Frame = 0


Query: 1   MGKCVLWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQ 60
           MGK VLWLAS+LLLV AA       E+QLSEPGG  P+ DI DPKYQ+LAK+ VEIHNWQ
Sbjct: 1   MGKSVLWLASLLLLVVAA----SGVEEQLSEPGGLKPV-DISDPKYQSLAKITVEIHNWQ 60

Query: 61  ANSHLHFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVDPTDNISIVFFNL 120
             S+L F E VCGWS+I +G  YQFCLRLIDKDN+NA   Y   V +DP+ NI I  F+L
Sbjct: 61  VGSNLQFLELVCGWSRIVRGINYQFCLRLIDKDNNNAVFHYKIDVTLDPSANIFINDFHL 120

Query: 121 VPNDLECCY 130
           VP DLECC+
Sbjct: 121 VPIDLECCF 124

[Close]

BLAST of MS026429 vs. ExPASy TrEMBL
Match: A0A6J1IGE9 (cysteine proteinase inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111474507 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 3.5e-07
Identity = 44/123 (35.77%), Postives = 66/123 (53.66%), Query Frame = 0


Query: 1   MGKCVLWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQ 60
           M K + ++AS+LL V +   +   A  +L   G   PI +ID+PK Q+L +  V  HN Q
Sbjct: 1   MAKALFFVASLLLYVLSQS-SLAVATQRLDLVGSYKPIKNIDEPKIQSLGEFAVNEHNKQ 60

Query: 61  ANSHLHFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVDPTDNISIVFFNL 120
           A + LHFQ+ + G  QI  GT Y   LRL   +  +++  Y T V  D  +  +++ F  
Sbjct: 61  AKTQLHFQKVISGEMQIVAGTNYN--LRLTALEGTDSRT-YGTLVFTDLNNKNNLINFFD 119

Query: 121 VPN 124
           VPN
Sbjct: 121 VPN 119

[Close]

BLAST of MS026429 vs. ExPASy TrEMBL
Match: A0A6J1IJX9 (cysteine proteinase inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111475533 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.9e-06
Identity = 43/123 (34.96%), Postives = 65/123 (52.85%), Query Frame = 0


Query: 1   MGKCVLWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQ 60
           M K + ++AS+LL V +   +   A  +L   G   PI +ID+PK Q+L +  V  HN Q
Sbjct: 1   MAKALFFVASLLLYVLSQS-SLAVATQRLDLVGSYKPIKNIDEPKIQSLGEFAVNEHNKQ 60

Query: 61  ANSHLHFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVDPTDNISIVFFNL 120
           A + LHFQ+ + G  QI  GT Y   LRL   +  +++  Y T V  D  +  +++ F  
Sbjct: 61  AKTQLHFQKVISGEMQIVAGTNYN--LRLTALEGTDSRT-YGTLVFTDLNNKNNLINFFG 119

Query: 121 VPN 124
           V N
Sbjct: 121 VSN 119

[Close]

BLAST of MS026429 vs. ExPASy TrEMBL
Match: A0A6J1IC30 (cysteine proteinase inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471626 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 5.0e-06
Identity = 42/119 (35.29%), Postives = 61/119 (51.26%), Query Frame = 0


Query: 1   MGKCVLWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQ 60
           M K +  + S+ L V +   +  AA  +L   G   PI DIDDPK Q+L +  V  HN Q
Sbjct: 1   MAKALFLVISLFLYVLSLS-SLAAATQRLDLAGSYKPIKDIDDPKIQSLGEFAVNEHNKQ 60

Query: 61  ANSHLHFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVDPTDNISIVFFN 120
           A + L FQ+ + G  QI  GT Y   LRL   +  +++  Y T V  D  +  +++ FN
Sbjct: 61  AKTQLQFQKVISGEMQIVAGTNYN--LRLTALEGTSSRT-YGTLVFTDLNNKNNLINFN 115

[Close]

BLAST of MS026429 vs. ExPASy TrEMBL
Match: A0A6J1EMY2 (cysteine proteinase inhibitor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434041 PE=4 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 5.5e-05
Identity = 40/118 (33.90%), Postives = 60/118 (50.85%), Query Frame = 0


Query: 1   MGKCVLWLASVLLLVAAAGLANGAAEDQLSEPGGRYPIDDIDDPKYQTLAKLVVEIHNWQ 60
           M K +  +AS+LL V +   +  AA  +L   G   PI +IDDPK Q+L +  V  HN Q
Sbjct: 1   MTKALFLVASLLLYVLSLS-SLVAATQRLDLAGSYKPIKNIDDPKIQSLGEFAVNEHNKQ 60

Query: 61  ANSHLHFQEAVCGWSQITKGTLYQFCLRLIDKDNDNAKVRYITQVVVDPTDNISIVFF 119
           A + L FQ+ + G  QI  GT Y+  L  ++  +      Y T V  D  +  +++ F
Sbjct: 61  AKTQLKFQKVISGEMQIVAGTNYKLQLTALEGTSSGT---YGTLVFTDLNNKNNLINF 114

The following BLAST results are available for this feature:

BLAST of MS026429 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142678.1	4.3e-36	63.57	cystatin-1-like [Momordica charantia]	[more]
XP_023520258.1	5.5e-07	36.44	cysteine proteinase inhibitor 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022975330.1	7.2e-07	35.77	cysteine proteinase inhibitor 1-like [Cucurbita maxima]	[more]
KAG6583736.1	2.1e-06	34.96	Cysteine proteinase inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023520817.1	2.7e-06	35.04	cysteine proteinase inhibitor 1-like [Cucurbita pepo subsp. pepo] >XP_023521297....	[more]

BLAST of MS026429 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6TPK4	1.4e-05	34.95	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
P86472	3.1e-05	33.98	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]

BLAST of MS026429 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CNV2	2.1e-36	63.57	cystatin-1-like OS=Momordica charantia OX=3673 GN=LOC111012734 PE=4 SV=1	[more]
A0A6J1IGE9	3.5e-07	35.77	cysteine proteinase inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111474507...	[more]
A0A6J1IJX9	2.9e-06	34.96	cysteine proteinase inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111475533...	[more]
A0A6J1IC30	5.0e-06	35.29	cysteine proteinase inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471626...	[more]
A0A6J1EMY2	5.5e-05	33.90	cysteine proteinase inhibitor 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114340...	[more]

BLAST of MS026429 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 38..112 e-value: 4.2E-10 score: 39.8
None	No IPR available	GENE3D	3.10.450.10		coord: 31..123 e-value: 5.8E-13 score: 50.9
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 32..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS026429.1	MS026429.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity