MS026424 (gene) Bitter gourd (TR) v1

Overview
NameMS026424
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionBerberine bridge enzyme-like
Locationscaffold402: 1230715 .. 1232337 (+)
RNA-Seq ExpressionMS026424
SyntenyMS026424
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTCTCCAATTACCTTGTTAGCTTATACTTTTGTTGTCTTGTTTGTATGGGCAGCTTCTGCTCACAACCATGACGATTTTCTTCATTGTCTTTCTCTTCATTCTCCTAATGGCTCATCAATTTCAAAGATTATTTACACTCCAACTAATCCTTCATACTCTTCAGTTTTGAACTTCTCAACTCATAACTTAAGATTCACAACTTCCGAAACCCCAAAGCCTCAAGTCATTGTCACACCATTGCAAGTTTCACAAGTTCAAGCATCCATCGTTTGTGCCAAAAACAATGGGTTTCAGATACGAACACGAAGTGGCGGCCACGACTATGAGGGTCTTTCTTATGTCTCTGACGTCTCCTTCATCGTCGTCGATCTAATAAACCTTCGGTCAATCAATATTGACGTGGAAAAGAAATTTGCATGGGTCCAATCTGGAGCCACCATTGGTGAACTCTATTATAGAATTGCAGAGAAAAGCAAAACTTTGGGGTTCCCAGCCGGGGTTTGCCCCACGGTCGGTGTTGGTGGGCATTTCAGCGGTGGTGGTTATGGTTTGATGCTACGGAAATTCGGCCTCGCTGCTGATAATGTGGTTGACGCTTACTTCATTGACGTCAACGGTAAACTACACGACCGAAAATCAATGGGAGAGGATGTTTTTTGGGCTCTTAGAGGAGGAGGTGGAGCAAGCTTTGGAATTGTTCTTGCATGGAAAATAAAGCTTCTCCCCGTTCCACCGGTGGTGACTGTTTTCACAGTTACAAGAAATTTGGAACAAAACGCCACCGACCTTGTCCACCGGTGGCAATACGTGGCCGACAAACAATATGACAAACTTTTCATCAGAATTATATTAAGAGGCGTGAACTCAACTGATCAATATGGGGAAAAAAGGACAATTGAAGCAGCTTTCAACTCTTTATTCCTCGGAAAAGTCGAAAAGCTTCTTTCAATAATGCACAAGACTTTCCCAGAGTTGGGACTGGCAAAAGAAGATTGCATACAAATGAGTTGGATTGAGTCCATTCTCTATTTTGCCGGCTTTCCTAGAGGGGAGCCATTGGAGGTTCTTCTCGATAGAAGGCCATTGGTCCCCAAAAGATTCTTCAAAGCCAAATCAGACTATGTAAAGGAGCCAATCCCCAAAGCTGGGCTTGAGGGCATATGGAAATTATTCAACGAAGAAGAAGCTGAAGCAGCAGAGATGATTCTGAGCCCTTACGGTGGAAAAATGAATGAGATTTCTGAATCAGAGATTCCCTTTCCGCATAGAGCTGGAAATCTTTACAAGATTCAACATTTGGTGTATTGGGAAGAAGAAGGCGAAGATGTTGCACAAAGACATATAAATTGGATTCGTAAGCTTTACAGTTATGTGGCACCTTTCGTCTCGAAGAATCCAAGAGCGGCATATATCAATTATAGAGACTTAGATATTGGGACGAACAACAAGAATGGGAACACGAGCTATGGCGAAGCAAGCATTTGGGGAGTAAAATATTTCAAGGGCAATTTTAAGAGGCTGGTGAGTGTGAAAACTAAGATCGATCCTTCTAACTTCTTCAGAAATGAGCAGAGCATTCCTTCTCTTTTGTCATGGTGGAAGAAGAGGGGACTTTAG

mRNA sequence

ATGAAGTCTCCAATTACCTTGTTAGCTTATACTTTTGTTGTCTTGTTTGTATGGGCAGCTTCTGCTCACAACCATGACGATTTTCTTCATTGTCTTTCTCTTCATTCTCCTAATGGCTCATCAATTTCAAAGATTATTTACACTCCAACTAATCCTTCATACTCTTCAGTTTTGAACTTCTCAACTCATAACTTAAGATTCACAACTTCCGAAACCCCAAAGCCTCAAGTCATTGTCACACCATTGCAAGTTTCACAAGTTCAAGCATCCATCGTTTGTGCCAAAAACAATGGGTTTCAGATACGAACACGAAGTGGCGGCCACGACTATGAGGGTCTTTCTTATGTCTCTGACGTCTCCTTCATCGTCGTCGATCTAATAAACCTTCGGTCAATCAATATTGACGTGGAAAAGAAATTTGCATGGGTCCAATCTGGAGCCACCATTGGTGAACTCTATTATAGAATTGCAGAGAAAAGCAAAACTTTGGGGTTCCCAGCCGGGGTTTGCCCCACGGTCGGTGTTGGTGGGCATTTCAGCGGTGGTGGTTATGGTTTGATGCTACGGAAATTCGGCCTCGCTGCTGATAATGTGGTTGACGCTTACTTCATTGACGTCAACGGTAAACTACACGACCGAAAATCAATGGGAGAGGATGTTTTTTGGGCTCTTAGAGGAGGAGGTGGAGCAAGCTTTGGAATTGTTCTTGCATGGAAAATAAAGCTTCTCCCCGTTCCACCGGTGGTGACTGTTTTCACAGTTACAAGAAATTTGGAACAAAACGCCACCGACCTTGTCCACCGGTGGCAATACGTGGCCGACAAACAATATGACAAACTTTTCATCAGAATTATATTAAGAGGCGTGAACTCAACTGATCAATATGGGGAAAAAAGGACAATTGAAGCAGCTTTCAACTCTTTATTCCTCGGAAAAGTCGAAAAGCTTCTTTCAATAATGCACAAGACTTTCCCAGAGTTGGGACTGGCAAAAGAAGATTGCATACAAATGAGTTGGATTGAGTCCATTCTCTATTTTGCCGGCTTTCCTAGAGGGGAGCCATTGGAGGTTCTTCTCGATAGAAGGCCATTGGTCCCCAAAAGATTCTTCAAAGCCAAATCAGACTATGTAAAGGAGCCAATCCCCAAAGCTGGGCTTGAGGGCATATGGAAATTATTCAACGAAGAAGAAGCTGAAGCAGCAGAGATGATTCTGAGCCCTTACGGTGGAAAAATGAATGAGATTTCTGAATCAGAGATTCCCTTTCCGCATAGAGCTGGAAATCTTTACAAGATTCAACATTTGGTGTATTGGGAAGAAGAAGGCGAAGATGTTGCACAAAGACATATAAATTGGATTCGTAAGCTTTACAGTTATGTGGCACCTTTCGTCTCGAAGAATCCAAGAGCGGCATATATCAATTATAGAGACTTAGATATTGGGACGAACAACAAGAATGGGAACACGAGCTATGGCGAAGCAAGCATTTGGGGAGTAAAATATTTCAAGGGCAATTTTAAGAGGCTGGTGAGTGTGAAAACTAAGATCGATCCTTCTAACTTCTTCAGAAATGAGCAGAGCATTCCTTCTCTTTTGTCATGGTGGAAGAAGAGGGGACTTTAG

Coding sequence (CDS)

ATGAAGTCTCCAATTACCTTGTTAGCTTATACTTTTGTTGTCTTGTTTGTATGGGCAGCTTCTGCTCACAACCATGACGATTTTCTTCATTGTCTTTCTCTTCATTCTCCTAATGGCTCATCAATTTCAAAGATTATTTACACTCCAACTAATCCTTCATACTCTTCAGTTTTGAACTTCTCAACTCATAACTTAAGATTCACAACTTCCGAAACCCCAAAGCCTCAAGTCATTGTCACACCATTGCAAGTTTCACAAGTTCAAGCATCCATCGTTTGTGCCAAAAACAATGGGTTTCAGATACGAACACGAAGTGGCGGCCACGACTATGAGGGTCTTTCTTATGTCTCTGACGTCTCCTTCATCGTCGTCGATCTAATAAACCTTCGGTCAATCAATATTGACGTGGAAAAGAAATTTGCATGGGTCCAATCTGGAGCCACCATTGGTGAACTCTATTATAGAATTGCAGAGAAAAGCAAAACTTTGGGGTTCCCAGCCGGGGTTTGCCCCACGGTCGGTGTTGGTGGGCATTTCAGCGGTGGTGGTTATGGTTTGATGCTACGGAAATTCGGCCTCGCTGCTGATAATGTGGTTGACGCTTACTTCATTGACGTCAACGGTAAACTACACGACCGAAAATCAATGGGAGAGGATGTTTTTTGGGCTCTTAGAGGAGGAGGTGGAGCAAGCTTTGGAATTGTTCTTGCATGGAAAATAAAGCTTCTCCCCGTTCCACCGGTGGTGACTGTTTTCACAGTTACAAGAAATTTGGAACAAAACGCCACCGACCTTGTCCACCGGTGGCAATACGTGGCCGACAAACAATATGACAAACTTTTCATCAGAATTATATTAAGAGGCGTGAACTCAACTGATCAATATGGGGAAAAAAGGACAATTGAAGCAGCTTTCAACTCTTTATTCCTCGGAAAAGTCGAAAAGCTTCTTTCAATAATGCACAAGACTTTCCCAGAGTTGGGACTGGCAAAAGAAGATTGCATACAAATGAGTTGGATTGAGTCCATTCTCTATTTTGCCGGCTTTCCTAGAGGGGAGCCATTGGAGGTTCTTCTCGATAGAAGGCCATTGGTCCCCAAAAGATTCTTCAAAGCCAAATCAGACTATGTAAAGGAGCCAATCCCCAAAGCTGGGCTTGAGGGCATATGGAAATTATTCAACGAAGAAGAAGCTGAAGCAGCAGAGATGATTCTGAGCCCTTACGGTGGAAAAATGAATGAGATTTCTGAATCAGAGATTCCCTTTCCGCATAGAGCTGGAAATCTTTACAAGATTCAACATTTGGTGTATTGGGAAGAAGAAGGCGAAGATGTTGCACAAAGACATATAAATTGGATTCGTAAGCTTTACAGTTATGTGGCACCTTTCGTCTCGAAGAATCCAAGAGCGGCATATATCAATTATAGAGACTTAGATATTGGGACGAACAACAAGAATGGGAACACGAGCTATGGCGAAGCAAGCATTTGGGGAGTAAAATATTTCAAGGGCAATTTTAAGAGGCTGGTGAGTGTGAAAACTAAGATCGATCCTTCTAACTTCTTCAGAAATGAGCAGAGCATTCCTTCTCTTTTGTCATGGTGGAAGAAGAGGGGACTTTAG

Protein sequence

MKSPITLLAYTFVVLFVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLNFSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVSFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWKIKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGEKRTIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVLLDRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISESEIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDIGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWKKRGL
Homology
BLAST of MS026424 vs. NCBI nr
Match: XP_022152432.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 529/540 (97.96%), Postives = 533/540 (98.70%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVLFVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLNF 60
           MKSPITLLAYTFVVLFV AASAHNH+DFLHCLSLHSPNGSSIS IIYTPTNPSYSSVLNF
Sbjct: 1   MKSPITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNF 60

Query: 61  STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS 120
           STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS
Sbjct: 61  STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS 120

Query: 121 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 180
           FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS
Sbjct: 121 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 180

Query: 181 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWKI 240
           GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWA+RGGGGASFGIVLAWKI
Sbjct: 181 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKI 240

Query: 241 KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGEKRT 300
           KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQ DKLFIRIILRGVNSTDQYGEKRT
Sbjct: 241 KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRT 300

Query: 301 IEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVLLD 360
           IEAAFNSLFLGKVEKLLSIMHKTFP LGLAKEDCIQMSWIESILYFAGFPRGE LEVLLD
Sbjct: 301 IEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLD 360

Query: 361 RRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISESEI 420
           RRPLVPKRFFKAKSDYV EPIPK GLEGIWKLFNEE+AEAAEMILSPYGGKMNEISESEI
Sbjct: 361 RRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEI 420

Query: 421 PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI 480
           PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI
Sbjct: 421 PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI 480

Query: 481 GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWKKRGL 540
           GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWW+KRGL
Sbjct: 481 GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEKRGL 540

BLAST of MS026424 vs. NCBI nr
Match: XP_004135722.1 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653302.1 hypothetical protein Csa_023170 [Cucumis sativus])

HSP 1 Score: 959.5 bits (2479), Expect = 1.2e-275
Identity = 460/542 (84.87%), Postives = 500/542 (92.25%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLN 60
           MKS IT  A+ F+VL F WAASAHNH+ FLHCLS HSPN SSISKIIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFAHIFIVLTFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRFT+ +TPKPQVIVTP  VSQ+QASI+CAKN GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SF+VVDLINLRSI++D E   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNV+DAYFIDVNGKLHDRKSMGEDVFWA+RG GGAS+GIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTD-QYGEK 300
           IKLLPVPP+VTVFT+ R LEQNATD++HRWQYV+ KQ DKLFIRIIL G+N+T+ Q+G K
Sbjct: 241 IKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVL 360
           RTIEAAFNSLFLGK+E+L+ IM KTFPELGL KEDCI+MSWIES+LYFAGF RG+PL VL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISES 420
           LDRRPL PKRFFKAKSDYV EPIPKAGLEGIW+ FNEEEAEAA +ILSPYGG M++ISES
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYW+EEGED+A+RHINWIRKLYSY+APFVSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNNKNG-NTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWKK 540
           DIGTN KNG NTSY EAS+WG+KYFKGNFKRLVSVKTK+DPSNFF+NEQSIPSL  WW K
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGIKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

BLAST of MS026424 vs. NCBI nr
Match: XP_038895082.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 957.2 bits (2473), Expect = 6.0e-275
Identity = 466/542 (85.98%), Postives = 500/542 (92.25%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLN 60
           MKS IT  A  F+VL F WAASA NH+ FLHCLSLH PN SSISKIIYTPTNPSYSSVLN
Sbjct: 1   MKSQITSFACIFIVLSFAWAASADNHEAFLHCLSLHYPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRFT+ +TPKPQVI+TP QV QVQASI+CAKN GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPQVIITPFQVFQVQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            F+VVDLINLRSI++DVE   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFVVVDLINLRSISVDVENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNV+DAYFIDV+GKLHDRKSMGEDVFWA+RGGGGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVDGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTD-QYGEK 300
           IKLLPVPP VTVFTVTRNLEQNAT+L+++WQY++ KQ + LFIRIILRG+N+T+ Q G K
Sbjct: 241 IKLLPVPPTVTVFTVTRNLEQNATNLINQWQYISSKQDENLFIRIILRGINTTNSQDGNK 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVL 360
           RTIEAAFNSLFLGK+E+LL IM +TFPELGL KEDCI+MSWIESILYFAGF RG+PL VL
Sbjct: 301 RTIEAAFNSLFLGKIEQLLQIMQETFPELGLTKEDCIEMSWIESILYFAGFSRGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISES 420
           LDRRPL PKRFFKAKSDYV EPIPKAGLEGIW+LFNEEEAEAAEMILSPYGG M++ISES
Sbjct: 361 LDRRPLFPKRFFKAKSDYVNEPIPKAGLEGIWELFNEEEAEAAEMILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           EIPFPHR GNLYKIQHLVYWEEEGED+AQRHINWIRKLYSY+APFVSKNPR AYINYRDL
Sbjct: 421 EIPFPHREGNLYKIQHLVYWEEEGEDIAQRHINWIRKLYSYMAPFVSKNPRLAYINYRDL 480

Query: 481 DIGTNNKNG-NTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWKK 540
           DIG N KNG NTSY EAS+WGVKYFKGNFKRLVSVKTKIDPSNFF+NEQSIPSLL WWKK
Sbjct: 481 DIGMNEKNGRNTSYSEASVWGVKYFKGNFKRLVSVKTKIDPSNFFKNEQSIPSLLPWWKK 540

BLAST of MS026424 vs. NCBI nr
Match: XP_008445653.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >KAA0047121.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK24891.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 955.3 bits (2468), Expect = 2.3e-274
Identity = 460/542 (84.87%), Postives = 499/542 (92.07%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLN 60
           MKS IT   + F+VL F WAASAHNH+ FLHCLS HSPN SSISKIIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFLHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRFT+ +TPKP +IVTP  VSQ+QASI+CAK  GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPHIIVTPFLVSQIQASIICAKKTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SF+VVDLINLRSI++D E   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNV+DAYFIDVNGKLHDRKSMGEDVFWA+RGGGGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTD-QYGEK 300
           IKLLPVP +VTVFT+TR LEQNATDL+HRWQYV+ KQ DKLFIRIIL G+N+T+ Q+G K
Sbjct: 241 IKLLPVPSIVTVFTITRTLEQNATDLIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVL 360
           RTIEAAFNSLFLGK+E+L+ IM KTFPELGL KEDCI+MSWIES+LYFAGF RG+PL VL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMEKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISES 420
           LDRRPL PKRFFKAKSDYV EPIPKAGLEGIW+LF+EEEAEAA +ILSPYGG M++ISES
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWELFDEEEAEAAVLILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYW+EEGED+A+RHINWIRKLYSY+APFVSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNNKNG-NTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWKK 540
           DIGTN KNG NTSY EAS+WGVKYFKGNFKRLVSVKTK+DPSNFF+NEQSIPSL  WW K
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

BLAST of MS026424 vs. NCBI nr
Match: XP_022954564.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 954.9 bits (2467), Expect = 3.0e-274
Identity = 459/541 (84.84%), Postives = 495/541 (91.50%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLN 60
           MK PITL  YTFVVL F WAASAHNH+DFLHCLSLHSPN SSISKIIYTP + SYSSVLN
Sbjct: 1   MKPPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKIIYTPNDSSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRF T +TPKPQVIVTPLQV++++ASI+CAK  GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFATPQTPKPQVIVTPLQVTEIKASIICAKTTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            FIVVDLINLRSIN+D E   AWVQSGATIGELYYRIA+KSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFIVVDLINLRSINVDAENNVAWVQSGATIGELYYRIAKKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLA DNV+DAYFIDVNG LHDRKSMGEDVFWALRGGGGASFGIV+AWK
Sbjct: 181 SGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTD-QYGEK 300
           IKLL VPP+VTVFTVTRNLEQNAT+L+HRWQY+A KQ D+LFIRII+RGVNST+ Q+G +
Sbjct: 241 IKLLQVPPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQHGSQ 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVL 360
           RT+EAAFNS+FLGK E+LL IM  TFPELGL KEDCI+MSWIESILYFAGFP G+PL VL
Sbjct: 301 RTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISES 420
           LDR PL PKRFFKAKSDYV EPIPKA LEG W+LFNEEEAEAAEMILSPYGG M+EISES
Sbjct: 361 LDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           E PFPHRAGNLYKIQHLVYWEEEGED+A+RHINWIRKLYS+V P+VSKNPRAAYINYRDL
Sbjct: 421 ETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYRDL 480

Query: 481 DIGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWKKR 540
           DIG NNKNG TSY EAS+WGVKYFKGNFKRLVS+KTK+DPSNFF NEQSIPS+L  WKKR
Sbjct: 481 DIGMNNKNGKTSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPSWKKR 540

BLAST of MS026424 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 6.6e-160
Identity = 273/511 (53.42%), Postives = 371/511 (72.60%), Query Frame = 0

Query: 19  AASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLNFSTHNLRFTTSETPKPQVI 78
           +A+  N   FL CLSL   + + +SK+I+TP + S+SSVL  S  N RF+  + PKP +I
Sbjct: 28  SANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLI 87

Query: 79  VTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVSFIVVDLINLRSINIDVEK 138
           +TP+Q S VQ+++ CA+  G  IRTRSGGHDYEGLSYV+   F+++DL NLRSI +DV+ 
Sbjct: 88  LTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDN 147

Query: 139 KFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADNV 198
           +  WVQ+GATIGELYY I +K++TL FPAGVCPTVGVGGHFSGGGYG +LRK GLAAD+V
Sbjct: 148 RSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHV 207

Query: 199 VDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWKIKLLPVPPVVTVFTVTRNL 258
           +DA  +D  G++ +R+ MGED FWA+RGGGG+SF +VL+WKI L+ VP  VTVF VT+  
Sbjct: 208 IDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFS 267

Query: 259 EQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGEKRTIEAAFNSLFLGKVEKLLS 318
           EQ+A  ++HRWQ+VADK  D LFIR++L+      +Y  K  + A+F  L+LG V+ LL 
Sbjct: 268 EQSALKIIHRWQFVADKVSDDLFIRVMLQ------RY--KNMVRASFPGLYLGSVKNLLK 327

Query: 319 IMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVLLDRRPLVPKRFFKAKSDYVK 378
           +++K FPELGL ++DC +MSWIES+++FA     EP+ VL  R        FKAKSD+V+
Sbjct: 328 MVNKEFPELGLEEDDCTEMSWIESVIWFAELGE-EPINVLTKRTR--ASLAFKAKSDFVQ 387

Query: 379 EPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISESEIPFPHRAGNLYKIQHLVYW 438
           EP+PK  +  +W+   E EAE A++I +P+GGKM+EI++ E PFPHR GN+Y+IQ+L YW
Sbjct: 388 EPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW 447

Query: 439 EEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDIGTNNKNGNTSYGEASIWG 498
                DV ++++ W+ ++Y  ++ FV+K+PR AYIN RDLD+G       + Y E   WG
Sbjct: 448 R---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWG 507

Query: 499 VKYFKGNFKRLVSVKTKIDPSNFFRNEQSIP 530
           VKYFK NF+RLV VKT +DPS+FF +EQSIP
Sbjct: 508 VKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of MS026424 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 6.2e-158
Identity = 273/533 (51.22%), Postives = 368/533 (69.04%), Query Frame = 0

Query: 5   ITLLAYTFVVLFVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTN--PSYSSVLNFST 64
           I +L ++   +   ++ A   D F++C+  ++     + K  + PT     +S VL  + 
Sbjct: 12  ILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTA 71

Query: 65  HNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVS--DVS 124
            NLRF     PKP  I +P+  S VQASI+C+K     +R RSGGHDYEGLSYVS  D  
Sbjct: 72  QNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKP 131

Query: 125 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 184
           FI++DL  +R +NI+++   AWVQSGAT+GELYYRIAEKSK  GFPAG+C ++G+GGH +
Sbjct: 132 FILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHIT 191

Query: 185 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWKI 244
           GG YG M+RK+GL ADNV+DA  +D NGKL DR +MGED FWA+RGG G SFGI+LAWKI
Sbjct: 192 GGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKI 251

Query: 245 KLLPVPPVVTVFTVTRNLEQN-ATDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGEKR 304
           KL+PVP  VTVFTVT+ L+Q+    ++ +WQ VADK  ++LFIR++     +    G  +
Sbjct: 252 KLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT----GGNK 311

Query: 305 TIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVLL 364
           T+  ++N+LFLG    L+++M K+FPELGL  +DCI+MSW+ESI Y +GFP   P  VLL
Sbjct: 312 TVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLL 371

Query: 365 DRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISESE 424
             +   PK  FKAKSD+VK PIP++GL+GI+K   +E+     MI +PYGG M +I ES+
Sbjct: 372 QGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQ 431

Query: 425 IPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLD 484
           IPFPHR G L+K+Q++  W  + +    RHINWIR LYSY+ P+VS NPR AY+NYRDLD
Sbjct: 432 IPFPHRKGVLFKVQYVTSW-LDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLD 491

Query: 485 IGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLL 533
           +G N K+  T   +A +WG  YFK NF RL+ +K K+DP NFFR+EQSIP ++
Sbjct: 492 LGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPMM 537

BLAST of MS026424 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.4e-157
Identity = 276/533 (51.78%), Postives = 372/533 (69.79%), Query Frame = 0

Query: 2   KSPITLLAYTFVVLFVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPS--YSSVLN 61
           K   TL   T +++ V  +S+    DF+ CL  +S     I+   ++P   +  +   L 
Sbjct: 7   KRNATLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELE 66

Query: 62  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVS-- 121
            +  NLR+ T   PKP  I  PL  + VQA++VCAK     +R RSGGHDYEGLS+V+  
Sbjct: 67  STAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAED 126

Query: 122 DVSFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGG 181
           +  F++VDL  LR +++D++   AW  +GATIGE+YYRI EKS+T GFPAG+C ++G+GG
Sbjct: 127 ETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGG 186

Query: 182 HFSGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLA 241
           H  GG YG M+RKFGL ADNV+DA  +D NG++ DR +MGEDVFWA+RGGGG SFG++LA
Sbjct: 187 HLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILA 246

Query: 242 WKIKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGE 301
           WKIKL+PVP  VTVFTVT+ LEQ+ T ++++W+ +ADK  D LFIR+I+   + T + G 
Sbjct: 247 WKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPG- 306

Query: 302 KRTIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEV 361
            RTI  ++ + FLG   +LL +M K+FPELGL K+DC +MSWI+S++Y AGFP     E 
Sbjct: 307 NRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEA 366

Query: 362 LLDRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISE 421
           LL  + L  K  FKAKSD+VKEPIP  GLEG+W+ F EE++     I +PYGG M+ ISE
Sbjct: 367 LLAGKSLF-KNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRISE 426

Query: 422 SEIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRD 481
           SEIPFPHR G L+KIQ L  W ++G+   +RH+ WIR++YSY+  +VSKNPR AY+NYRD
Sbjct: 427 SEIPFPHRNGTLFKIQWLSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRD 486

Query: 482 LDIGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPS 531
           LD+GTN   G T   +A  WG KY+KGNF+RLV +K + DP NFFR+EQS+P+
Sbjct: 487 LDLGTN--EGET---DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of MS026424 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 6.9e-157
Identity = 270/532 (50.75%), Postives = 366/532 (68.80%), Query Frame = 0

Query: 5   ITLLAYTFVVLFVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTN--PSYSSVLNFST 64
           +++L ++F  +   ++SA   D F++C+  ++     + K ++TP      ++ VL  + 
Sbjct: 15  VSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLESTA 74

Query: 65  HNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS-- 124
            NL+F     PKP  I  P+  SQVQASI+C+K  G   R RSGGHD+E LSYVS +   
Sbjct: 75  QNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKP 134

Query: 125 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 184
           FI++DL  L+ IN+D+E   AWVQ GAT+GELYYRIAEKSK  GFPAG+C +VG+GG+ +
Sbjct: 135 FILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMT 194

Query: 185 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWKI 244
           GGGYG ++RK+GLA DNV+D   +D NGKL DR +MGED+FWA+RGGGGASFGIVLAWKI
Sbjct: 195 GGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKI 254

Query: 245 KLLPVPPVVTVFTVTRNLEQNA-TDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGEKR 304
           KL+PVP  VTVFTVT+ LEQ+A    + +WQ ++ K  +++ IR++LR   +       +
Sbjct: 255 KLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGNDG----NK 314

Query: 305 TIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVLL 364
           T+   +   FLG+   LL +M K FPELGL ++DC +MSWIE+ L+  GFP G P+E+LL
Sbjct: 315 TVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILL 374

Query: 365 DRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISESE 424
             +  + K +FKA SD+VKEPIP  GL+GI+K     E     +  +PYGG M++I ES 
Sbjct: 375 QLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRL--IEGNTTFLNWTPYGGMMSKIPESA 434

Query: 425 IPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLD 484
           IPFPHR G L+KI +   W  E +  + R INWI+++Y+Y+AP+VS NPR AY+NYRDLD
Sbjct: 435 IPFPHRNGTLFKILYYANW-LENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLD 494

Query: 485 IGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSL 532
            G N  N   ++ EA IWG KYFKGNF RLV +KTK+DP NFFR+EQSIP +
Sbjct: 495 FGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539

BLAST of MS026424 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 2.0e-156
Identity = 270/516 (52.33%), Postives = 369/516 (71.51%), Query Frame = 0

Query: 19  AASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLNFSTHNLRFTTSETPKPQVI 78
           +A   NH++FL CLS H  N    S+II+T  +PSY S+LN S  N RF   ETPKP  I
Sbjct: 28  SAQRTNHENFLKCLS-HRINEDD-SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSI 87

Query: 79  VTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVS-DVSFIVVDLINLRSINIDVE 138
           +TP+Q + VQ++I CA+ +G  IRTRSGGHDYEGLSY++    F+V+DL NLRSI +DV+
Sbjct: 88  ITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVD 147

Query: 139 KKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADN 198
            +  WVQSGATIGELYY I + SK+L FPAG+ PTVG+GG F GGGYG ++RK+GL+ADN
Sbjct: 148 NRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADN 207

Query: 199 VVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWKIKLLPVPPVVTVFTVTRN 258
           V+DA+ +D NG   DR+ MGED FWA+RGGGG+SF +VL+WKI+LL VP VVTVF V + 
Sbjct: 208 VIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKT 267

Query: 259 LEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGEKRTIEAAFNSLFLGKVEKLL 318
            E+ A  ++++WQY+ADK  + LFIR +L+         ++  + A+F  L+LG V  LL
Sbjct: 268 SEKEAVSIINKWQYIADKVPNDLFIRAMLQ---------KETEVYASFPGLYLGPVSDLL 327

Query: 319 SIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVLLDRRPLVPKRFFKAKSDYV 378
           ++M   FPELGL   +C +MSWIES+L+F    +GE +E+L  R+     R FK K D++
Sbjct: 328 ALMKDKFPELGLEIGNCREMSWIESVLWFI---KGESMEILAKRKR--TSRSFKGKDDFI 387

Query: 379 KEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISESEIPFPHRAGNLYKIQHLVY 438
           +EPIPK  ++ +W+ F   EA  A++IL+P+GGKM+EI+++EIPFPHR GNLY+IQ+L Y
Sbjct: 388 EEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAY 447

Query: 439 WEEE---GEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDIGTN-NKNGNTSYGE 498
           W EE    +   ++++ W+  +Y ++ P+VSK+PR AY+N+RD+D+G     N  T Y E
Sbjct: 448 WSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEE 507

Query: 499 ASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIP 530
           A +WGVKYFK NF RLV VKT +DP +FF +EQSIP
Sbjct: 508 AKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MS026424 vs. ExPASy TrEMBL
Match: A0A6J1DG91 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020167 PE=3 SV=1)

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 529/540 (97.96%), Postives = 533/540 (98.70%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVLFVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLNF 60
           MKSPITLLAYTFVVLFV AASAHNH+DFLHCLSLHSPNGSSIS IIYTPTNPSYSSVLNF
Sbjct: 1   MKSPITLLAYTFVVLFVSAASAHNHEDFLHCLSLHSPNGSSISNIIYTPTNPSYSSVLNF 60

Query: 61  STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS 120
           STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS
Sbjct: 61  STHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS 120

Query: 121 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 180
           FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS
Sbjct: 121 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 180

Query: 181 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWKI 240
           GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWA+RGGGGASFGIVLAWKI
Sbjct: 181 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKI 240

Query: 241 KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGEKRT 300
           KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQ DKLFIRIILRGVNSTDQYGEKRT
Sbjct: 241 KLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTDQYGEKRT 300

Query: 301 IEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVLLD 360
           IEAAFNSLFLGKVEKLLSIMHKTFP LGLAKEDCIQMSWIESILYFAGFPRGE LEVLLD
Sbjct: 301 IEAAFNSLFLGKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLD 360

Query: 361 RRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISESEI 420
           RRPLVPKRFFKAKSDYV EPIPK GLEGIWKLFNEE+AEAAEMILSPYGGKMNEISESEI
Sbjct: 361 RRPLVPKRFFKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEI 420

Query: 421 PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI 480
           PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI
Sbjct: 421 PFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDI 480

Query: 481 GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWKKRGL 540
           GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWW+KRGL
Sbjct: 481 GTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWEKRGL 540

BLAST of MS026424 vs. ExPASy TrEMBL
Match: A0A5A7U0J8 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5482G00070 PE=3 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 1.1e-274
Identity = 460/542 (84.87%), Postives = 499/542 (92.07%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLN 60
           MKS IT   + F+VL F WAASAHNH+ FLHCLS HSPN SSISKIIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFLHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRFT+ +TPKP +IVTP  VSQ+QASI+CAK  GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPHIIVTPFLVSQIQASIICAKKTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SF+VVDLINLRSI++D E   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNV+DAYFIDVNGKLHDRKSMGEDVFWA+RGGGGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTD-QYGEK 300
           IKLLPVP +VTVFT+TR LEQNATDL+HRWQYV+ KQ DKLFIRIIL G+N+T+ Q+G K
Sbjct: 241 IKLLPVPSIVTVFTITRTLEQNATDLIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVL 360
           RTIEAAFNSLFLGK+E+L+ IM KTFPELGL KEDCI+MSWIES+LYFAGF RG+PL VL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMEKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISES 420
           LDRRPL PKRFFKAKSDYV EPIPKAGLEGIW+LF+EEEAEAA +ILSPYGG M++ISES
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWELFDEEEAEAAVLILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYW+EEGED+A+RHINWIRKLYSY+APFVSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNNKNG-NTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWKK 540
           DIGTN KNG NTSY EAS+WGVKYFKGNFKRLVSVKTK+DPSNFF+NEQSIPSL  WW K
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

BLAST of MS026424 vs. ExPASy TrEMBL
Match: A0A1S3BE31 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488607 PE=3 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 1.1e-274
Identity = 460/542 (84.87%), Postives = 499/542 (92.07%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLN 60
           MKS IT   + F+VL F WAASAHNH+ FLHCLS HSPN SSISKIIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFLHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRFT+ +TPKP +IVTP  VSQ+QASI+CAK  GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPHIIVTPFLVSQIQASIICAKKTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
           SF+VVDLINLRSI++D E   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLAADNV+DAYFIDVNGKLHDRKSMGEDVFWA+RGGGGASFGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTD-QYGEK 300
           IKLLPVP +VTVFT+TR LEQNATDL+HRWQYV+ KQ DKLFIRIIL G+N+T+ Q+G K
Sbjct: 241 IKLLPVPSIVTVFTITRTLEQNATDLIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVL 360
           RTIEAAFNSLFLGK+E+L+ IM KTFPELGL KEDCI+MSWIES+LYFAGF RG+PL VL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMEKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISES 420
           LDRRPL PKRFFKAKSDYV EPIPKAGLEGIW+LF+EEEAEAA +ILSPYGG M++ISES
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWELFDEEEAEAAVLILSPYGGIMDKISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           EIPFPHRAGNLYKIQHLVYW+EEGED+A+RHINWIRKLYSY+APFVSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 481 DIGTNNKNG-NTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWKK 540
           DIGTN KNG NTSY EAS+WGVKYFKGNFKRLVSVKTK+DPSNFF+NEQSIPSL  WW K
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

BLAST of MS026424 vs. ExPASy TrEMBL
Match: A0A6J1GTC1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456799 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.4e-274
Identity = 459/541 (84.84%), Postives = 495/541 (91.50%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLN 60
           MK PITL  YTFVVL F WAASAHNH+DFLHCLSLHSPN SSISKIIYTP + SYSSVLN
Sbjct: 1   MKPPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKIIYTPNDSSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRF T +TPKPQVIVTPLQV++++ASI+CAK  GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFATPQTPKPQVIVTPLQVTEIKASIICAKTTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            FIVVDLINLRSIN+D E   AWVQSGATIGELYYRIA+KSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFIVVDLINLRSINVDAENNVAWVQSGATIGELYYRIAKKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLA DNV+DAYFIDVNG LHDRKSMGEDVFWALRGGGGASFGIV+AWK
Sbjct: 181 SGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTD-QYGEK 300
           IKLL VPP+VTVFTVTRNLEQNAT+L+HRWQY+A KQ D+LFIRII+RGVNST+ Q+G +
Sbjct: 241 IKLLQVPPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQHGSQ 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVL 360
           RT+EAAFNS+FLGK E+LL IM  TFPELGL KEDCI+MSWIESILYFAGFP G+PL VL
Sbjct: 301 RTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISES 420
           LDR PL PKRFFKAKSDYV EPIPKA LEG W+LFNEEEAEAAEMILSPYGG M+EISES
Sbjct: 361 LDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           E PFPHRAGNLYKIQHLVYWEEEGED+A+RHINWIRKLYS+V P+VSKNPRAAYINYRDL
Sbjct: 421 ETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYRDL 480

Query: 481 DIGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWKKR 540
           DIG NNKNG TSY EAS+WGVKYFKGNFKRLVS+KTK+DPSNFF NEQSIPS+L  WKKR
Sbjct: 481 DIGMNNKNGKTSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPSWKKR 540

BLAST of MS026424 vs. ExPASy TrEMBL
Match: A0A6J1K043 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490403 PE=3 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.7e-273
Identity = 457/540 (84.63%), Postives = 490/540 (90.74%), Query Frame = 0

Query: 1   MKSPITLLAYTFVVL-FVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLN 60
           MKSPITL  YTFVVL F WAASAHNH+DFLHCLSLHSPN SSISK IYTP + SYSSVLN
Sbjct: 1   MKSPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKTIYTPNDSSYSSVLN 60

Query: 61  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FS HNLRF T  TPKPQVIVTPLQVS+++ASI+CAK  GFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFATPRTPKPQVIVTPLQVSEIKASIICAKTTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 SFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180
            FI+VDLINLRSIN+D +   AWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 PFIIVDLINLRSINVDAKNNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 181 SGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWK 240
           SGGGYGLMLRKFGLA DNV+DAYFIDVNG LHDRKSMGEDVFWALRGGGGASFGIV+AWK
Sbjct: 181 SGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWK 240

Query: 241 IKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTD-QYGEK 300
           IKLL VPP+VTVFTV R LEQNAT+L+HRWQY+A KQ D+LFIRII+RGVNST+ Q G +
Sbjct: 241 IKLLQVPPIVTVFTVPRTLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQQGSQ 300

Query: 301 RTIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVL 360
           RT+EAAFNS+FLGK E+LL IM  TFPELGL KEDCI+MSWIESILYFAGFP G+PL VL
Sbjct: 301 RTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVL 360

Query: 361 LDRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISES 420
           LDR PL PKRFFKAKSDYV EPIPKA LEG W+LF EEEAEAAEMILSPYGG M+EISES
Sbjct: 361 LDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFKEEEAEAAEMILSPYGGIMDEISES 420

Query: 421 EIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDL 480
           E PFPHRAGNLYKIQHLVYWEEEGED+A+RHINWIRKLYSYVAP+VS NPRAAYINYRDL
Sbjct: 421 ETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSYVAPYVSNNPRAAYINYRDL 480

Query: 481 DIGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLLSWWKKR 539
           DIG NNKNG TSY EAS+WGVKYFKGNFKRLVS+KTK+DPSNFF NEQSIPS+L WWKKR
Sbjct: 481 DIGMNNKNGKTSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPWWKKR 540

BLAST of MS026424 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 565.5 bits (1456), Expect = 4.7e-161
Identity = 273/511 (53.42%), Postives = 371/511 (72.60%), Query Frame = 0

Query: 19  AASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLNFSTHNLRFTTSETPKPQVI 78
           +A+  N   FL CLSL   + + +SK+I+TP + S+SSVL  S  N RF+  + PKP +I
Sbjct: 28  SANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLI 87

Query: 79  VTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVSFIVVDLINLRSINIDVEK 138
           +TP+Q S VQ+++ CA+  G  IRTRSGGHDYEGLSYV+   F+++DL NLRSI +DV+ 
Sbjct: 88  LTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDN 147

Query: 139 KFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADNV 198
           +  WVQ+GATIGELYY I +K++TL FPAGVCPTVGVGGHFSGGGYG +LRK GLAAD+V
Sbjct: 148 RSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHV 207

Query: 199 VDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWKIKLLPVPPVVTVFTVTRNL 258
           +DA  +D  G++ +R+ MGED FWA+RGGGG+SF +VL+WKI L+ VP  VTVF VT+  
Sbjct: 208 IDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFS 267

Query: 259 EQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGEKRTIEAAFNSLFLGKVEKLLS 318
           EQ+A  ++HRWQ+VADK  D LFIR++L+      +Y  K  + A+F  L+LG V+ LL 
Sbjct: 268 EQSALKIIHRWQFVADKVSDDLFIRVMLQ------RY--KNMVRASFPGLYLGSVKNLLK 327

Query: 319 IMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVLLDRRPLVPKRFFKAKSDYVK 378
           +++K FPELGL ++DC +MSWIES+++FA     EP+ VL  R        FKAKSD+V+
Sbjct: 328 MVNKEFPELGLEEDDCTEMSWIESVIWFAELGE-EPINVLTKRTR--ASLAFKAKSDFVQ 387

Query: 379 EPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISESEIPFPHRAGNLYKIQHLVYW 438
           EP+PK  +  +W+   E EAE A++I +P+GGKM+EI++ E PFPHR GN+Y+IQ+L YW
Sbjct: 388 EPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW 447

Query: 439 EEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDIGTNNKNGNTSYGEASIWG 498
                DV ++++ W+ ++Y  ++ FV+K+PR AYIN RDLD+G       + Y E   WG
Sbjct: 448 R---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWG 507

Query: 499 VKYFKGNFKRLVSVKTKIDPSNFFRNEQSIP 530
           VKYFK NF+RLV VKT +DPS+FF +EQSIP
Sbjct: 508 VKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of MS026424 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 558.9 bits (1439), Expect = 4.4e-159
Identity = 273/533 (51.22%), Postives = 368/533 (69.04%), Query Frame = 0

Query: 5   ITLLAYTFVVLFVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTN--PSYSSVLNFST 64
           I +L ++   +   ++ A   D F++C+  ++     + K  + PT     +S VL  + 
Sbjct: 12  ILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTA 71

Query: 65  HNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVS--DVS 124
            NLRF     PKP  I +P+  S VQASI+C+K     +R RSGGHDYEGLSYVS  D  
Sbjct: 72  QNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKP 131

Query: 125 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 184
           FI++DL  +R +NI+++   AWVQSGAT+GELYYRIAEKSK  GFPAG+C ++G+GGH +
Sbjct: 132 FILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHIT 191

Query: 185 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWKI 244
           GG YG M+RK+GL ADNV+DA  +D NGKL DR +MGED FWA+RGG G SFGI+LAWKI
Sbjct: 192 GGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKI 251

Query: 245 KLLPVPPVVTVFTVTRNLEQN-ATDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGEKR 304
           KL+PVP  VTVFTVT+ L+Q+    ++ +WQ VADK  ++LFIR++     +    G  +
Sbjct: 252 KLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT----GGNK 311

Query: 305 TIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVLL 364
           T+  ++N+LFLG    L+++M K+FPELGL  +DCI+MSW+ESI Y +GFP   P  VLL
Sbjct: 312 TVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLL 371

Query: 365 DRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISESE 424
             +   PK  FKAKSD+VK PIP++GL+GI+K   +E+     MI +PYGG M +I ES+
Sbjct: 372 QGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQ 431

Query: 425 IPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLD 484
           IPFPHR G L+K+Q++  W  + +    RHINWIR LYSY+ P+VS NPR AY+NYRDLD
Sbjct: 432 IPFPHRKGVLFKVQYVTSW-LDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLD 491

Query: 485 IGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSLL 533
           +G N K+  T   +A +WG  YFK NF RL+ +K K+DP NFFR+EQSIP ++
Sbjct: 492 LGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPMM 537

BLAST of MS026424 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 557.8 bits (1436), Expect = 9.8e-159
Identity = 276/533 (51.78%), Postives = 372/533 (69.79%), Query Frame = 0

Query: 2   KSPITLLAYTFVVLFVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPS--YSSVLN 61
           K   TL   T +++ V  +S+    DF+ CL  +S     I+   ++P   +  +   L 
Sbjct: 7   KRNATLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELE 66

Query: 62  FSTHNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVS-- 121
            +  NLR+ T   PKP  I  PL  + VQA++VCAK     +R RSGGHDYEGLS+V+  
Sbjct: 67  STAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAED 126

Query: 122 DVSFIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGG 181
           +  F++VDL  LR +++D++   AW  +GATIGE+YYRI EKS+T GFPAG+C ++G+GG
Sbjct: 127 ETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGG 186

Query: 182 HFSGGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLA 241
           H  GG YG M+RKFGL ADNV+DA  +D NG++ DR +MGEDVFWA+RGGGG SFG++LA
Sbjct: 187 HLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILA 246

Query: 242 WKIKLLPVPPVVTVFTVTRNLEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGE 301
           WKIKL+PVP  VTVFTVT+ LEQ+ T ++++W+ +ADK  D LFIR+I+   + T + G 
Sbjct: 247 WKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPG- 306

Query: 302 KRTIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEV 361
            RTI  ++ + FLG   +LL +M K+FPELGL K+DC +MSWI+S++Y AGFP     E 
Sbjct: 307 NRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEA 366

Query: 362 LLDRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISE 421
           LL  + L  K  FKAKSD+VKEPIP  GLEG+W+ F EE++     I +PYGG M+ ISE
Sbjct: 367 LLAGKSLF-KNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRISE 426

Query: 422 SEIPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRD 481
           SEIPFPHR G L+KIQ L  W ++G+   +RH+ WIR++YSY+  +VSKNPR AY+NYRD
Sbjct: 427 SEIPFPHRNGTLFKIQWLSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRD 486

Query: 482 LDIGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPS 531
           LD+GTN   G T   +A  WG KY+KGNF+RLV +K + DP NFFR+EQS+P+
Sbjct: 487 LDLGTN--EGET---DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of MS026424 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 555.4 bits (1430), Expect = 4.9e-158
Identity = 270/532 (50.75%), Postives = 366/532 (68.80%), Query Frame = 0

Query: 5   ITLLAYTFVVLFVWAASAHNHDDFLHCLSLHSPNGSSISKIIYTPTN--PSYSSVLNFST 64
           +++L ++F  +   ++SA   D F++C+  ++     + K ++TP      ++ VL  + 
Sbjct: 15  VSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLESTA 74

Query: 65  HNLRFTTSETPKPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVS-- 124
            NL+F     PKP  I  P+  SQVQASI+C+K  G   R RSGGHD+E LSYVS +   
Sbjct: 75  QNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKP 134

Query: 125 FIVVDLINLRSINIDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 184
           FI++DL  L+ IN+D+E   AWVQ GAT+GELYYRIAEKSK  GFPAG+C +VG+GG+ +
Sbjct: 135 FILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMT 194

Query: 185 GGGYGLMLRKFGLAADNVVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWKI 244
           GGGYG ++RK+GLA DNV+D   +D NGKL DR +MGED+FWA+RGGGGASFGIVLAWKI
Sbjct: 195 GGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKI 254

Query: 245 KLLPVPPVVTVFTVTRNLEQNA-TDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGEKR 304
           KL+PVP  VTVFTVT+ LEQ+A    + +WQ ++ K  +++ IR++LR   +       +
Sbjct: 255 KLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGNDG----NK 314

Query: 305 TIEAAFNSLFLGKVEKLLSIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVLL 364
           T+   +   FLG+   LL +M K FPELGL ++DC +MSWIE+ L+  GFP G P+E+LL
Sbjct: 315 TVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILL 374

Query: 365 DRRPLVPKRFFKAKSDYVKEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISESE 424
             +  + K +FKA SD+VKEPIP  GL+GI+K     E     +  +PYGG M++I ES 
Sbjct: 375 QLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRL--IEGNTTFLNWTPYGGMMSKIPESA 434

Query: 425 IPFPHRAGNLYKIQHLVYWEEEGEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLD 484
           IPFPHR G L+KI +   W  E +  + R INWI+++Y+Y+AP+VS NPR AY+NYRDLD
Sbjct: 435 IPFPHRNGTLFKILYYANW-LENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLD 494

Query: 485 IGTNNKNGNTSYGEASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIPSL 532
            G N  N   ++ EA IWG KYFKGNF RLV +KTK+DP NFFR+EQSIP +
Sbjct: 495 FGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539

BLAST of MS026424 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 553.9 bits (1426), Expect = 1.4e-157
Identity = 270/516 (52.33%), Postives = 369/516 (71.51%), Query Frame = 0

Query: 19  AASAHNHDDFLHCLSLHSPNGSSISKIIYTPTNPSYSSVLNFSTHNLRFTTSETPKPQVI 78
           +A   NH++FL CLS H  N    S+II+T  +PSY S+LN S  N RF   ETPKP  I
Sbjct: 28  SAQRTNHENFLKCLS-HRINEDD-SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSI 87

Query: 79  VTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVS-DVSFIVVDLINLRSINIDVE 138
           +TP+Q + VQ++I CA+ +G  IRTRSGGHDYEGLSY++    F+V+DL NLRSI +DV+
Sbjct: 88  ITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVD 147

Query: 139 KKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADN 198
            +  WVQSGATIGELYY I + SK+L FPAG+ PTVG+GG F GGGYG ++RK+GL+ADN
Sbjct: 148 NRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADN 207

Query: 199 VVDAYFIDVNGKLHDRKSMGEDVFWALRGGGGASFGIVLAWKIKLLPVPPVVTVFTVTRN 258
           V+DA+ +D NG   DR+ MGED FWA+RGGGG+SF +VL+WKI+LL VP VVTVF V + 
Sbjct: 208 VIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKT 267

Query: 259 LEQNATDLVHRWQYVADKQYDKLFIRIILRGVNSTDQYGEKRTIEAAFNSLFLGKVEKLL 318
            E+ A  ++++WQY+ADK  + LFIR +L+         ++  + A+F  L+LG V  LL
Sbjct: 268 SEKEAVSIINKWQYIADKVPNDLFIRAMLQ---------KETEVYASFPGLYLGPVSDLL 327

Query: 319 SIMHKTFPELGLAKEDCIQMSWIESILYFAGFPRGEPLEVLLDRRPLVPKRFFKAKSDYV 378
           ++M   FPELGL   +C +MSWIES+L+F    +GE +E+L  R+     R FK K D++
Sbjct: 328 ALMKDKFPELGLEIGNCREMSWIESVLWFI---KGESMEILAKRKR--TSRSFKGKDDFI 387

Query: 379 KEPIPKAGLEGIWKLFNEEEAEAAEMILSPYGGKMNEISESEIPFPHRAGNLYKIQHLVY 438
           +EPIPK  ++ +W+ F   EA  A++IL+P+GGKM+EI+++EIPFPHR GNLY+IQ+L Y
Sbjct: 388 EEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAY 447

Query: 439 WEEE---GEDVAQRHINWIRKLYSYVAPFVSKNPRAAYINYRDLDIGTN-NKNGNTSYGE 498
           W EE    +   ++++ W+  +Y ++ P+VSK+PR AY+N+RD+D+G     N  T Y E
Sbjct: 448 WSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEE 507

Query: 499 ASIWGVKYFKGNFKRLVSVKTKIDPSNFFRNEQSIP 530
           A +WGVKYFK NF RLV VKT +DP +FF +EQSIP
Sbjct: 508 AKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152432.10.0e+0097.96berberine bridge enzyme-like 15 [Momordica charantia][more]
XP_004135722.11.2e-27584.87berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653302.1 hypothetical pro... [more]
XP_038895082.16.0e-27585.98berberine bridge enzyme-like 18 [Benincasa hispida][more]
XP_008445653.12.3e-27484.87PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >KAA0047121.... [more]
XP_022954564.13.0e-27484.84berberine bridge enzyme-like 18 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SVG56.6e-16053.42Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=... [more]
Q9FKU86.2e-15851.22Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=... [more]
O647431.4e-15751.78Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV... [more]
Q9FKV06.9e-15750.75Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=... [more]
Q9FI252.0e-15652.33Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1DG910.0e+0097.96berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020167 P... [more]
A0A5A7U0J81.1e-27484.87Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BE311.1e-27484.87tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348860... [more]
A0A6J1GTC11.4e-27484.84berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456799 PE... [more]
A0A6J1K0432.7e-27384.63berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490403 PE=3... [more]
Match NameE-valueIdentityDescription
AT4G20820.14.7e-16153.42FAD-binding Berberine family protein [more]
AT5G44400.14.4e-15951.22FAD-binding Berberine family protein [more]
AT2G34790.19.8e-15951.78FAD-binding Berberine family protein [more]
AT5G44380.14.9e-15850.75FAD-binding Berberine family protein [more]
AT5G44410.11.4e-15752.33FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 471..529
e-value: 8.8E-23
score: 80.2
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 133..529
e-value: 4.2E-172
score: 574.8
NoneNo IPR availableGENE3D3.40.462.20coord: 248..467
e-value: 4.2E-172
score: 574.8
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 22..531
NoneNo IPR availablePANTHERPTHR32448:SF113BERBERINE BRIDGE ENZYME-LIKE 18coord: 22..531
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 75..211
e-value: 1.1E-26
score: 93.3
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 22..129
e-value: 1.6E-43
score: 148.9
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 71..245
score: 17.493464
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 16..246

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS026424.1MS026424.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity