MS026415 (gene) Bitter gourd (TR) v1

Overview


Name	MS026415
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Berberine bridge enzyme-like
Location	scaffold402: 1152753 .. 1153196 (-)
RNA-Seq Expression	MS026415
Synteny	MS026415

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTCTCCAGTTACCTCCTTGGCTTATATTTTTGTTGTCTGGTATTTTGCATCGGTAACTTCTGCTCACAACCATGAAGATTTTCTTCAATGTATTTCTTCTCATTTCACCGACCATACTTCAATTTCAAAGATTATTTTTACTCTAACAGATCCTTCATACCCTTCGATTTTAACATTTTCAATCCAGAACCTAAGATTCTTGGGTGCCAAAACCCCAAAGTCTCAAGTAATCGTGACACCATCGCAAGTTTCCCACATCCAGGCATCTATTATTTGTGCAAAAAACAACGGCTTTCAGATACGAACACGAAGTGGTGGCCATGACTACGAAGGTCTTTCCTATGTTTCAGACGTTCCTTTCATCGTCGTTGAGCTGAGGAACCTGCGGTCGATTGATATTGATGTAGAAAAAGAAGTTGCATGGGTGGCCAAATCCTGA

mRNA sequence

ATGAAGTCTCCAGTTACCTCCTTGGCTTATATTTTTGTTGTCTGGTATTTTGCATCGGTAACTTCTGCTCACAACCATGAAGATTTTCTTCAATGTATTTCTTCTCATTTCACCGACCATACTTCAATTTCAAAGATTATTTTTACTCTAACAGATCCTTCATACCCTTCGATTTTAACATTTTCAATCCAGAACCTAAGATTCTTGGGTGCCAAAACCCCAAAGTCTCAAGTAATCGTGACACCATCGCAAGTTTCCCACATCCAGGCATCTATTATTTGTGCAAAAAACAACGGCTTTCAGATACGAACACGAAGTGGTGGCCATGACTACGAAGGTCTTTCCTATGTTTCAGACGTTCCTTTCATCGTCGTTGAGCTGAGGAACCTGCGGTCGATTGATATTGATGTAGAAAAAGAAGTTGCATGGGTGGCCAAATCCTGA

Coding sequence (CDS)

ATGAAGTCTCCAGTTACCTCCTTGGCTTATATTTTTGTTGTCTGGTATTTTGCATCGGTAACTTCTGCTCACAACCATGAAGATTTTCTTCAATGTATTTCTTCTCATTTCACCGACCATACTTCAATTTCAAAGATTATTTTTACTCTAACAGATCCTTCATACCCTTCGATTTTAACATTTTCAATCCAGAACCTAAGATTCTTGGGTGCCAAAACCCCAAAGTCTCAAGTAATCGTGACACCATCGCAAGTTTCCCACATCCAGGCATCTATTATTTGTGCAAAAAACAACGGCTTTCAGATACGAACACGAAGTGGTGGCCATGACTACGAAGGTCTTTCCTATGTTTCAGACGTTCCTTTCATCGTCGTTGAGCTGAGGAACCTGCGGTCGATTGATATTGATGTAGAAAAAGAAGTTGCATGGGTGGCCAAATCCTGA

Protein sequence

MKSPVTSLAYIFVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILTFSIQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDVPFIVVELRNLRSIDIDVEKEVAWVAKS

Homology

[Close]

BLAST of MS026415 vs. NCBI nr
Match: XP_022152442.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 220.3 bits (560), Expect = 1.1e-53
Identity = 110/144 (76.39%), Postives = 123/144 (85.42%), Query Frame = 0


Query: 1   MKSPVTSLAYIFVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILT 60
           MKSP++SLAYIFVV  F+ V SA+NHEDFL C+S HF + +SISKII+T TDP Y S+L 
Sbjct: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60

Query: 61  FSIQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FSIQNLRFLG KT K QVIVTP Q SHIQASIICAK NGFQIR RSGGHDYEGLSYVSDV
Sbjct: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120

Query: 121 PFIVVELRNLRSIDIDVEKEVAWV 145
           PF+VV++ NLRSID+DVEK VAWV
Sbjct: 121 PFVVVDMINLRSIDVDVEKRVAWV 144

[Close]

BLAST of MS026415 vs. NCBI nr
Match: XP_022152425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 219.2 bits (557), Expect = 2.4e-53
Identity = 108/144 (75.00%), Postives = 124/144 (86.11%), Query Frame = 0


Query: 1   MKSPVTSLAYIFVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILT 60
           MKSP+ SLAYIFVV  FA V SA N+EDFL C+S HF + +SISKII+T T PSY  +L 
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSSSISKIIYTPTHPSYSLVLN 60

Query: 61  FSIQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FSIQNLRFLGAKTPK Q+IVTP + SH+QASIICAKNNG QIRTRSGGHDYEGLSYVS+V
Sbjct: 61  FSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVSNV 120

Query: 121 PFIVVELRNLRSIDIDVEKEVAWV 145
           PF+VV+L NLRSID+DVE++VAWV
Sbjct: 121 PFVVVDLTNLRSIDVDVEQKVAWV 144

[Close]

BLAST of MS026415 vs. NCBI nr
Match: XP_022152520.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 213.0 bits (541), Expect = 1.7e-51
Identity = 105/144 (72.92%), Postives = 122/144 (84.72%), Query Frame = 0


Query: 1   MKSPVTSLAYIFVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILT 60
           MKSP+TSLAYIF ++  A V SA NHEDFL C+S H  + + IS II+T TDPSY S+L 
Sbjct: 1   MKSPITSLAYIF-IFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60

Query: 61  FSIQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FSIQN RFLGAKTPK Q+IVTP + SHIQAS+ICAKNNGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 PFIVVELRNLRSIDIDVEKEVAWV 145
           PF++V+L N+RSID+DVE+ VAWV
Sbjct: 121 PFVLVDLTNIRSIDVDVEQNVAWV 143

[Close]

BLAST of MS026415 vs. NCBI nr
Match: XP_022152423.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 212.6 bits (540), Expect = 2.3e-51
Identity = 110/145 (75.86%), Postives = 124/145 (85.52%), Query Frame = 0


Query: 1   MKSPVTSLAYIFVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILT 60
           MKSP+TSLAYIFVV  F  V SA N+EDFL+C+S H  +++SISKI++T  D SY SIL 
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 60

Query: 61  FSIQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSD- 120
           FSIQNLRF  AKTPK QVIVTP QVSHIQASIICAK+NGFQIRTRSGGHDYEGLSYVSD 
Sbjct: 61  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 120

Query: 121 VPFIVVELRNLRSIDIDVEKEVAWV 145
           VPF+V++L NLRSID+DVEK VAWV
Sbjct: 121 VPFVVIDLTNLRSIDVDVEKNVAWV 145

[Close]

BLAST of MS026415 vs. NCBI nr
Match: XP_022152426.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 211.5 bits (537), Expect = 5.0e-51
Identity = 104/144 (72.22%), Postives = 123/144 (85.42%), Query Frame = 0


Query: 1   MKSPVTSLAYIFVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILT 60
           MKSP+TSLAYIFVV +F+ V SA+N E+F+ C+S H  + +SI  II+T TDPSY SIL 
Sbjct: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60

Query: 61  FSIQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           +SI N RF+GAKTPK QVIVTP QVSHIQASIICA+ NGFQIRTRSGGHDYEG+SYVSDV
Sbjct: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120

Query: 121 PFIVVELRNLRSIDIDVEKEVAWV 145
           PF++V+  NLRSIDIDVE++VAWV
Sbjct: 121 PFVLVDFVNLRSIDIDVERKVAWV 144

[Close]

BLAST of MS026415 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 7.6e-34
Identity = 70/133 (52.63%), Postives = 94/133 (70.68%), Query Frame = 0


Query: 12  FVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILTFSIQNLRFLGA 71
           F + +   ++ A+  E+FL+C S H  ++ +  K+++T  D  Y SIL  +IQNLRF+  
Sbjct: 17  FFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISD 76

Query: 72  KTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDVPFIVVELRNLR 131
            TPK  VIVTPS  SHIQA+I+C+K  G QIRTRSGGHD EG+SY+S VPF+VV+LRN+ 
Sbjct: 77  TTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMH 136

Query: 132 SIDIDVEKEVAWV 145
           SI IDV  + AWV
Sbjct: 137 SIKIDVHSQTAWV 149

[Close]

BLAST of MS026415 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 4.9e-33
Identity = 71/135 (52.59%), Postives = 95/135 (70.37%), Query Frame = 0


Query: 11  IFVVWYFASVTS-AHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILTFSIQNLRFL 70
           IF  + F   TS A+  E+FL+C S +  ++ +  K+++T  +P Y S+L  +I NLRF 
Sbjct: 15  IFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFT 74

Query: 71  GAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDVPFIVVELRN 130
              TPK  VIVTPS VSHIQ +I+C+K  G QIRTRSGGHD EG+SY+S VPF++V+LRN
Sbjct: 75  SDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRN 134

Query: 131 LRSIDIDVEKEVAWV 145
           +RSI IDV  + AWV
Sbjct: 135 MRSIKIDVHSQTAWV 149

[Close]

BLAST of MS026415 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.1e-32
Identity = 68/136 (50.00%), Postives = 95/136 (69.85%), Query Frame = 0


Query: 13  VVWYFAS----VTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILTFSIQNLRF 72
           ++++F S    ++ A+  E+FL+C S +  ++ +  K I+T  D  Y S+L  +IQNLRF
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRF 73

Query: 73  LGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDVPFIVVELR 132
               TPK  VIVTPS VSHIQASI+C+K  G QIRTRSGGHD EGLSY+S VPF +V+LR
Sbjct: 74  TSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLR 133

Query: 133 NLRSIDIDVEKEVAWV 145
           N+ ++ +D+  + AWV
Sbjct: 134 NMHTVKVDIHSQTAWV 149

[Close]

BLAST of MS026415 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 3.2e-32
Identity = 66/136 (48.53%), Postives = 95/136 (69.85%), Query Frame = 0


Query: 13  VVWYFAS----VTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILTFSIQNLRF 72
           ++++F S    ++ A+  E+FL+C S +   + + +K+++T  D  Y SIL  +IQNLRF
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRF 73

Query: 73  LGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDVPFIVVELR 132
               TPK  VI+TP  VSHIQ +I+C+K  G QIRTRSGGHD EG+SY+S VPF++V+LR
Sbjct: 74  TSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLR 133

Query: 133 NLRSIDIDVEKEVAWV 145
           N+ S+ IDV  + AWV
Sbjct: 134 NMHSVKIDVHSQTAWV 149

[Close]

BLAST of MS026415 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 5.5e-32
Identity = 65/136 (47.79%), Postives = 95/136 (69.85%), Query Frame = 0


Query: 13  VVWYFAS----VTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILTFSIQNLRF 72
           ++++F S    ++ A+  E+FL+C+S +   + + +K+++T  D  Y SIL  ++QNLRF
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRF 73

Query: 73  LGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDVPFIVVELR 132
               TPK  VI TP  VSHIQ +I+C+K  G QIRTRSGGHD EG+SY+S VPF++V+LR
Sbjct: 74  TSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLR 133

Query: 133 NLRSIDIDVEKEVAWV 145
           N+ S+ IDV  + AWV
Sbjct: 134 NMHSVKIDVHSQTAWV 149

[Close]

BLAST of MS026415 vs. ExPASy TrEMBL
Match: A0A6J1DG99 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020173 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 5.3e-54
Identity = 110/144 (76.39%), Postives = 123/144 (85.42%), Query Frame = 0


Query: 1   MKSPVTSLAYIFVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILT 60
           MKSP++SLAYIFVV  F+ V SA+NHEDFL C+S HF + +SISKII+T TDP Y S+L 
Sbjct: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDSSISKIIYTPTDPLYFSVLN 60

Query: 61  FSIQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FSIQNLRFLG KT K QVIVTP Q SHIQASIICAK NGFQIR RSGGHDYEGLSYVSDV
Sbjct: 61  FSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSDV 120

Query: 121 PFIVVELRNLRSIDIDVEKEVAWV 145
           PF+VV++ NLRSID+DVEK VAWV
Sbjct: 121 PFVVVDMINLRSIDVDVEKRVAWV 144

[Close]

BLAST of MS026415 vs. ExPASy TrEMBL
Match: A0A6J1DDW6 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 PE=3 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.2e-53
Identity = 108/144 (75.00%), Postives = 124/144 (86.11%), Query Frame = 0


Query: 1   MKSPVTSLAYIFVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILT 60
           MKSP+ SLAYIFVV  FA V SA N+EDFL C+S HF + +SISKII+T T PSY  +L 
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSSSISKIIYTPTHPSYSLVLN 60

Query: 61  FSIQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FSIQNLRFLGAKTPK Q+IVTP + SH+QASIICAKNNG QIRTRSGGHDYEGLSYVS+V
Sbjct: 61  FSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVSNV 120

Query: 121 PFIVVELRNLRSIDIDVEKEVAWV 145
           PF+VV+L NLRSID+DVE++VAWV
Sbjct: 121 PFVVVDLTNLRSIDVDVEQKVAWV 144

[Close]

BLAST of MS026415 vs. ExPASy TrEMBL
Match: A0A6J1DE62 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020226 PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 8.4e-52
Identity = 105/144 (72.92%), Postives = 122/144 (84.72%), Query Frame = 0


Query: 1   MKSPVTSLAYIFVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILT 60
           MKSP+TSLAYIF ++  A V SA NHEDFL C+S H  + + IS II+T TDPSY S+L 
Sbjct: 1   MKSPITSLAYIF-IFLSAWVASACNHEDFLDCLSIHSPNSSPISNIIYTPTDPSYSSVLN 60

Query: 61  FSIQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           FSIQN RFLGAKTPK Q+IVTP + SHIQAS+ICAKNNGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSDV 120

Query: 121 PFIVVELRNLRSIDIDVEKEVAWV 145
           PF++V+L N+RSID+DVE+ VAWV
Sbjct: 121 PFVLVDLTNIRSIDVDVEQNVAWV 143

[Close]

BLAST of MS026415 vs. ExPASy TrEMBL
Match: A0A6J1DET9 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020159 PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.1e-51
Identity = 110/145 (75.86%), Postives = 124/145 (85.52%), Query Frame = 0


Query: 1   MKSPVTSLAYIFVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILT 60
           MKSP+TSLAYIFVV  F  V SA N+EDFL+C+S H  +++SISKI++T  D SY SIL 
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 60

Query: 61  FSIQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSD- 120
           FSIQNLRF  AKTPK QVIVTP QVSHIQASIICAK+NGFQIRTRSGGHDYEGLSYVSD 
Sbjct: 61  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSDN 120

Query: 121 VPFIVVELRNLRSIDIDVEKEVAWV 145
           VPF+V++L NLRSID+DVEK VAWV
Sbjct: 121 VPFVVIDLTNLRSIDVDVEKNVAWV 145

[Close]

BLAST of MS026415 vs. ExPASy TrEMBL
Match: A0A6J1DHP3 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020162 PE=3 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.4e-51
Identity = 104/144 (72.22%), Postives = 123/144 (85.42%), Query Frame = 0


Query: 1   MKSPVTSLAYIFVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILT 60
           MKSP+TSLAYIFVV +F+ V SA+N E+F+ C+S H  + +SI  II+T TDPSY SIL 
Sbjct: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTSSILNIIYTSTDPSYSSILN 60

Query: 61  FSIQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDV 120
           +SI N RF+GAKTPK QVIVTP QVSHIQASIICA+ NGFQIRTRSGGHDYEG+SYVSDV
Sbjct: 61  YSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSDV 120

Query: 121 PFIVVELRNLRSIDIDVEKEVAWV 145
           PF++V+  NLRSIDIDVE++VAWV
Sbjct: 121 PFVLVDFVNLRSIDIDVERKVAWV 144

[Close]

BLAST of MS026415 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 129.8 bits (325), Expect = 1.8e-30
Identity = 61/120 (50.83%), Postives = 80/120 (66.67%), Query Frame = 0


Query: 25  NHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILTFSIQNLRFLGAKTPKSQVIVTPSQ 84
           N   FLQC+S    D   +SK+I T  D S+ S+L  SIQN RF     PK  +I+TP Q
Sbjct: 33  NQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQ 92

Query: 85  VSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVSDVPFIVVELRNLRSIDIDVEKEVAWV 144
            S +Q+++ CA+  G  IRTRSGGHDYEGLSYV+  PF++++LRNLRSI +DV+    WV
Sbjct: 93  PSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWV 152

[Close]

BLAST of MS026415 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 127.9 bits (320), Expect = 6.8e-30
Identity = 67/131 (51.15%), Postives = 91/131 (69.47%), Query Frame = 0


Query: 16  YFASVTSAH-NHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILTFSIQNLRFLGAKTP 75
           +F S+++   NHE+FL+C+S    +    S+II T  DPSY SIL  SIQN RF   +TP
Sbjct: 23  HFPSISAQRTNHENFLKCLSHRINEDD--SRIIHTSKDPSYFSILNSSIQNPRFFVLETP 82

Query: 76  KSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVS-DVPFIVVELRNLRSI 135
           K   I+TP Q + +Q++I CA+ +G  IRTRSGGHDYEGLSY++   PF+V++LRNLRSI
Sbjct: 83  KPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSI 142

Query: 136 DIDVEKEVAWV 145
            +DV+    WV
Sbjct: 143 TLDVDNRTGWV 151

[Close]

BLAST of MS026415 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 123.2 bits (308), Expect = 1.7e-28
Identity = 67/143 (46.85%), Postives = 93/143 (65.03%), Query Frame = 0


Query: 9   AYIFVVWYF----ASVTSAH--NHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILTFS 68
           +++F +  F    + + SAH  NHEDFL+C+S    D+T   K+I T  D S+ SIL  S
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 69  IQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYVS-DVP 128
           IQN RF  ++TPK   I+TP + S +Q  I CA+ +G  +RTRS GH YEGLSY++ + P
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 129 FIVVELRNLRSIDIDVEKEVAWV 145
           F V++LRNLRSI +DV+    WV
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWV 147

[Close]

BLAST of MS026415 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 120.2 bits (300), Expect = 1.4e-27
Identity = 63/139 (45.32%), Postives = 89/139 (64.03%), Query Frame = 0


Query: 8   LAYIFVVWYFASVTSAHNHEDFLQCISSHFTDHTSISKIIFTLTDPSYPSILTFSIQNLR 67
           L   FVV+ + S +S+ N E F QC++S+      IS  IF   + SY S+L  +I+NLR
Sbjct: 7   LVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLR 66

Query: 68  FLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLSYV--SDVPFIVV 127
           F    TPK  +I+  +  SH+QA+I C K +  Q++ RSGGHDY+GLSYV  S  PF V+
Sbjct: 67  FNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVL 126

Query: 128 ELRNLRSIDIDVEKEVAWV 145
           ++ NLRS+D+DV  + AWV
Sbjct: 127 DMFNLRSVDVDVASKTAWV 145

[Close]

BLAST of MS026415 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 111.3 bits (277), Expect = 6.6e-25
Identity = 55/150 (36.67%), Postives = 91/150 (60.67%), Query Frame = 0


Query: 6   TSLAYIFVVWYFASV---------TSAHNHEDFLQCISSHF-TDHTSISKIIFTLTDPSY 65
           T ++  F V++  S+         +S   +E F+QC S    +    I+  +F+ T+PS+
Sbjct: 6   TFVSVFFFVFFLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSF 65

Query: 66  PSILTFSIQNLRFLGAKTPKSQVIVTPSQVSHIQASIICAKNNGFQIRTRSGGHDYEGLS 125
            S+L   I+N RF  + TPK  +IVTP    H+ A++ C+K+  F ++ RSGGHDYEGLS
Sbjct: 66  SSVLRAYIRNGRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLS 125

Query: 126 YVSDVPFIVVELRNLRSIDIDVEKEVAWVA 146
           Y+SD PF ++++ NLR + +D+  + AW++
Sbjct: 126 YISDKPFFILDMSNLRDVSVDIADQSAWIS 155

The following BLAST results are available for this feature:

BLAST of MS026415 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152442.1	1.1e-53	76.39	berberine bridge enzyme-like 18 [Momordica charantia]	[more]
XP_022152425.1	2.4e-53	75.00	berberine bridge enzyme-like 18 [Momordica charantia]	[more]
XP_022152520.1	1.7e-51	72.92	berberine bridge enzyme-like 18 [Momordica charantia]	[more]
XP_022152423.1	2.3e-51	75.86	berberine bridge enzyme-like 15 [Momordica charantia]	[more]
XP_022152426.1	5.0e-51	72.22	berberine bridge enzyme-like 18 [Momordica charantia]	[more]

BLAST of MS026415 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GTB6	7.6e-34	52.63	Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV...	[more]
A6P6V9	4.9e-33	52.59	Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1	[more]
Q33DQ2	1.1e-32	50.00	Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1	[more]
A6P6W0	3.2e-32	48.53	Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=...	[more]
A6P6W1	5.5e-32	47.79	Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=...	[more]

BLAST of MS026415 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DG99	5.3e-54	76.39	berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020173 P...	[more]
A0A6J1DDW6	1.2e-53	75.00	berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 P...	[more]
A0A6J1DE62	8.4e-52	72.92	berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020226 P...	[more]
A0A6J1DET9	1.1e-51	75.86	berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020159 P...	[more]
A0A6J1DHP3	2.4e-51	72.22	berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020162 P...	[more]

BLAST of MS026415 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20820.1	1.8e-30	50.83	FAD-binding Berberine family protein	[more]
AT5G44410.1	6.8e-30	51.15	FAD-binding Berberine family protein	[more]
AT5G44440.1	1.7e-28	46.85	FAD-binding Berberine family protein	[more]
AT1G30700.1	1.4e-27	45.32	FAD-binding Berberine family protein	[more]
AT4G20840.1	6.6e-25	36.67	FAD-binding Berberine family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016167	FAD-binding, type PCMH, subdomain 1	GENE3D	3.30.43.10		coord: 23..130 e-value: 1.5E-41 score: 142.5
IPR006094	FAD linked oxidase, N-terminal	PFAM	PF01565	FAD_binding_4	coord: 77..144 e-value: 1.6E-8 score: 34.4
None	No IPR available	PANTHER	PTHR32448:SF76	CANNABIDIOLIC ACID SYNTHASE-LIKE 1	coord: 11..145
None	No IPR available	PANTHER	PTHR32448	OS08G0158400 PROTEIN	coord: 11..145
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 16..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS026415.1	MS026415.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016491	oxidoreductase activity