MS026400 (gene) Bitter gourd (TR) v1

BLAST of MS026400 vs. NCBI nr
Match: XP_022142587.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 392.9 bits (1008), Expect = 2.2e-105
Identity = 196/245 (80.00%), Postives = 222/245 (90.61%), Query Frame = 0

Query: 1   MKYSSPLIPLALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPD-SNFSISKVVYTPINS 60
           M Y SPLI L+LAF  LSSSS W  A  +K+E+FLQCLL+HS + SN+SISKV++TPINS
Sbjct: 1   MNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINS 60

Query: 61  SYSSILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEG 120
           SYSS+L+FSIRNLRFSTVETPKPLLI+TPSHVSHIQAAVICSK++G QIRTRSGGHDFEG
Sbjct: 61  SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG 120

Query: 121 LSYVAHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTM 180
           LSYVAHLPFIIVDL NL+SISIDIKDNTAW+QSGAT+GELY SIA+K+ TLAF A +   
Sbjct: 121 LSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPT 180

Query: 181 VGVGGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSF 240
           VGVGG FSGGGYG+LLRKYGLA DNV+DAYL+DA+GKF++RESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVA 245
           GIVVA
Sbjct: 241 GIVVA 245

BLAST of MS026400 vs. NCBI nr
Match: XP_022142425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 392.5 bits (1007), Expect = 2.8e-105
Identity = 194/242 (80.17%), Postives = 215/242 (88.84%), Query Frame = 0

Query: 3   YSSPLIPLALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSSYS 62
           YSSPLIPL LAF  LSSS  W  A ADK+E+FLQCLLNHSPD N+SISKV++TPINSSYS
Sbjct: 5   YSSPLIPLFLAFIFLSSSRFWTLAVADKHESFLQCLLNHSPDGNYSISKVIHTPINSSYS 64

Query: 63  SILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGLSY 122
           S+L+FSIRNLRFST ETPKPLLIITPSHVSHIQAAV+CSK +G QIRTRSGGHDFE LSY
Sbjct: 65  SVLDFSIRNLRFSTAETPKPLLIITPSHVSHIQAAVVCSKRYGFQIRTRSGGHDFERLSY 124

Query: 123 VAHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTMVGV 182
           VAHL FIIVDL NL+SIS+D+K+N AW+ SGAT+GELY SIA+K+ T AF A V   VGV
Sbjct: 125 VAHLSFIIVDLINLRSISVDVKNNNAWVHSGATLGELYYSIAQKSRTSAFPAGVCPTVGV 184

Query: 183 GGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 242
           GG FSGGGYG+LLRKYGLA DNV+DAYL+DANGKF+DRESMGEDLFWAIRGGGGGSFGIV
Sbjct: 185 GGHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIV 244

Query: 243 VA 245
           VA
Sbjct: 245 VA 246

BLAST of MS026400 vs. NCBI nr
Match: XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 390.2 bits (1001), Expect = 1.4e-104
Identity = 191/246 (77.64%), Postives = 217/246 (88.21%), Query Frame = 0

Query: 1   MKYSSPLIPL--ALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPIN 60
           MKYSSPLIPL  +L F  LSSSS W  AS DK+E+FL CLL+HSPDSN+SISKVVYTP+N
Sbjct: 1   MKYSSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLN 60

Query: 61  SSYSSILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFE 120
           SSYSS+L+FSIRNLRFST +TPKPL IITP HVSH+QAAV+CS+THGLQIRTRSGGHDFE
Sbjct: 61  SSYSSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFE 120

Query: 121 GLSYVAHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVST 180
           GLS V+HLPFII+DL NL+SI+ID  +NTAW+QSGAT+GELY SIAEK+ TLAF A    
Sbjct: 121 GLSCVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACP 180

Query: 181 MVGVGGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGS 240
            VG+GG FSGGGYG++LRK+GLA DNV+DAYL+DANGKF DRE MGEDLFWAIRGGGGGS
Sbjct: 181 TVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGS 240

Query: 241 FGIVVA 245
           FGIVVA
Sbjct: 241 FGIVVA 246

BLAST of MS026400 vs. NCBI nr
Match: XP_022152518.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 388.7 bits (997), Expect = 4.1e-104
Identity = 196/244 (80.33%), Postives = 221/244 (90.57%), Query Frame = 0

Query: 1   MKYSSPLIPLALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSS 60
           MKY SPL  L+LAF LLSSSSSW  ASA+K+EAFLQCL +HS DS+ SISKV+YTPIN S
Sbjct: 1   MKY-SPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSS-SISKVIYTPINPS 60

Query: 61  YSSILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGL 120
           YSS+LNFS+RNLRFS+ ETPKPLLIITPS+VSH+QAAVICSKTHGLQIR RSGGHD EGL
Sbjct: 61  YSSVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGL 120

Query: 121 SYVAHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTMV 180
           SYVAHLPFII+DL NLKSI+++I+++TAWIQSGAT+GELY +IA+K+ TLAF A V   V
Sbjct: 121 SYVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTV 180

Query: 181 GVGGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFG 240
           GVGG FSGGGYG++LRKYGLA DNV+DAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVA 245
           IVVA
Sbjct: 241 IVVA 242

BLAST of MS026400 vs. NCBI nr
Match: XP_038895508.1 (berberine bridge enzyme-like 28 [Benincasa hispida])

HSP 1 Score: 373.6 bits (958), Expect = 1.4e-99
Identity = 186/244 (76.23%), Postives = 213/244 (87.30%), Query Frame = 0

Query: 1   MKYSSPLIPLALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSS 60
           MKY+SPLI L+L   ++SSS  W  AS  KY+ FLQCLL HS D N+ ISKVVY+PINSS
Sbjct: 1   MKYTSPLILLSLVLIVVSSSPLWASASEQKYQHFLQCLLEHSLD-NYPISKVVYSPINSS 60

Query: 61  YSSILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGL 120
           YSS+L+FSIRNLRFS  +TPKPLLIITPSHVSHIQAAVICSK+HGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTMV 180
           SYVA+LPFIIVDL NL+S++ID++++TAW+QSGAT+GELY  I EK+ TLAF A V   V
Sbjct: 121 SYVAYLPFIIVDLINLRSVTIDVENSTAWVQSGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GVGGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFG 240
           GVGG FSGGGYG +LRK+GLA DNV+DAYL+DANGK +DRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGFMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVA 245
           IVVA
Sbjct: 241 IVVA 243

BLAST of MS026400 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 5.8e-69
Identity = 133/246 (54.07%), Postives = 177/246 (71.95%), Query Frame = 0

Query: 3   YSSPLIPLALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSSYS 62
           +SS LI    +  LLS     V A+     +FLQCL     DSN  +SKV++TP ++S+S
Sbjct: 7   FSSVLIFFTTSTLLLSIPHP-VSANRSNQSSFLQCLSLQLNDSNI-VSKVIHTPNDTSFS 66

Query: 63  SILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGLSY 122
           S+L  SI+N RFS  + PKP+LI+TP   S +Q+AV C++  G+ IRTRSGGHD+EGLSY
Sbjct: 67  SVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSY 126

Query: 123 VAHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTMVGV 182
           V H PF+I+DLRNL+SI++D+ + + W+Q+GAT+GELY  I +K  TLAF A V   VGV
Sbjct: 127 VTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGV 186

Query: 183 GGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 242
           GG FSGGGYG LLRK+GLA D+V+DA ++DA G+  +R  MGED FWAIRGGGG SF +V
Sbjct: 187 GGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVV 246

Query: 243 VACEAG 249
           ++ + G
Sbjct: 247 LSWKIG 250

BLAST of MS026400 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 5.8e-69
Identity = 126/217 (58.06%), Postives = 164/217 (75.58%), Query Frame = 0

Query: 28  ADKYEAFLQCLLNHSPDSNFSISKVVYTPINSSYSSILNFSIRNLRFSTVETPKPLLIIT 87
           A+  E FL+C   H P +N +  K+VYT  +  Y SILN +I+NLRF +  TPKPL+I+T
Sbjct: 28  ANPRENFLKCFSKHIP-NNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVT 87

Query: 88  PSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGLSYVAHLPFIIVDLRNLKSISIDIKDNT 147
           PS+ SHIQA ++CSK  GLQIRTRSGGHD EG+SY++ +PF++VDLRN+ SI ID+   T
Sbjct: 88  PSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQT 147

Query: 148 AWIQSGATVGELYNSIAEKTPTLAFAASVSTMVGVGGQFSGGGYGMLLRKYGLATDNVVD 207
           AW+++GAT+GE+Y  I EK   L+F       VGVGG FSGGGYG L+R YGLA DN++D
Sbjct: 148 AWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIID 207

Query: 208 AYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVA 245
           A+L++ +GK  DR+SMGEDLFWAIRGGGG +FGI+ A
Sbjct: 208 AHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAA 243

BLAST of MS026400 vs. ExPASy Swiss-Prot
Match: A0A2S1XB67 (O-acetylstemmadenine oxidase OS=Catharanthus roseus OX=4058 GN=ASO PE=1 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 7.5e-69
Identity = 134/241 (55.60%), Postives = 179/241 (74.27%), Query Frame = 0

Query: 8   IPLALAFF----LLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSSYSS 67
           +P+ L+ F    LLSSS   +       EAFL C+ N     + SI  +++ P NSSY S
Sbjct: 5   VPIVLSIFCFLLLLSSSHGSIP------EAFLNCISNKF-SLDVSILNILHVPSNSSYDS 64

Query: 68  ILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGLSYV 127
           +L  +I+N RF  +++PKPL IITP   SH+Q+AVIC+K  GLQIR RSGG D+EGLSY 
Sbjct: 65  VLKSTIQNPRF--LKSPKPLAIITPVLHSHVQSAVICTKQAGLQIRIRSGGADYEGLSYR 124

Query: 128 AHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTMVGVG 187
           + +PFI++DL+NL+SIS+DI+DN+AW++SGAT+GE Y+ IA+ +P  AF A VS+ VG+G
Sbjct: 125 SEVPFILLDLQNLRSISVDIEDNSAWVESGATIGEFYHEIAQNSPVHAFPAGVSSSVGIG 184

Query: 188 GQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVV 245
           G  S GG+G LLRKYGLA DN++DA ++DA G+  DRESMGEDLFWAIRGGGG SFG++V
Sbjct: 185 GHLSSGGFGTLLRKYGLAADNIIDAKIVDARGRILDRESMGEDLFWAIRGGGGASFGVIV 236

BLAST of MS026400 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 2.9e-68
Identity = 124/217 (57.14%), Postives = 163/217 (75.12%), Query Frame = 0

Query: 28  ADKYEAFLQCLLNHSPDSNFSISKVVYTPINSSYSSILNFSIRNLRFSTVETPKPLLIIT 87
           A+  E FL+C   + P +N +  K+VYT  N  Y S+LN +I NLRF++  TPKPL+I+T
Sbjct: 28  ANPRENFLKCFSQYIP-NNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVT 87

Query: 88  PSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGLSYVAHLPFIIVDLRNLKSISIDIKDNT 147
           PSHVSHIQ  ++CSK  GLQIRTRSGGHD EG+SY++ +PF+IVDLRN++SI ID+   T
Sbjct: 88  PSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQT 147

Query: 148 AWIQSGATVGELYNSIAEKTPTLAFAASVSTMVGVGGQFSGGGYGMLLRKYGLATDNVVD 207
           AW+++GAT+GE+Y  + EK   L+ AA     V  GG F GGGYG L+R YGLA DN++D
Sbjct: 148 AWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIID 207

Query: 208 AYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVA 245
           A+L++ +GK  DR+SMGEDLFWA+RGGG  SFGI+VA
Sbjct: 208 AHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVA 243

BLAST of MS026400 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 8.3e-68
Identity = 128/233 (54.94%), Postives = 171/233 (73.39%), Query Frame = 0

Query: 12  LAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSSYSSILNFSIRN 71
           + FF LS +     + A+  E FL+CL  + P +N + +K+VYT  +  Y SILN +++N
Sbjct: 14  IIFFFLSFNIQI--SIANPQENFLKCLSQYIP-TNVTNAKLVYTQHDQFYMSILNSTVQN 73

Query: 72  LRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGLSYVAHLPFIIV 131
           LRF++  TPKPL+I TP +VSHIQ  ++CSK  GLQIRTRSGGHD EG+SY++ +PF+IV
Sbjct: 74  LRFTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIV 133

Query: 132 DLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTMVGVGGQFSGGGY 191
           DLRN+ S+ ID+   TAW++SGAT+GE+Y  I E    L+F A     VG GG FSGGGY
Sbjct: 134 DLRNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGY 193

Query: 192 GMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVA 245
           G L+R YGLA DN++DA+L++ +GK  DR+SMGEDLFWAIRGGGG +FGI+ A
Sbjct: 194 GALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAA 243

BLAST of MS026400 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 1.0e-105
Identity = 196/245 (80.00%), Postives = 222/245 (90.61%), Query Frame = 0

Query: 1   MKYSSPLIPLALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPD-SNFSISKVVYTPINS 60
           M Y SPLI L+LAF  LSSSS W  A  +K+E+FLQCLL+HS + SN+SISKV++TPINS
Sbjct: 1   MNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINS 60

Query: 61  SYSSILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEG 120
           SYSS+L+FSIRNLRFSTVETPKPLLI+TPSHVSHIQAAVICSK++G QIRTRSGGHDFEG
Sbjct: 61  SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG 120

Query: 121 LSYVAHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTM 180
           LSYVAHLPFIIVDL NL+SISIDIKDNTAW+QSGAT+GELY SIA+K+ TLAF A +   
Sbjct: 121 LSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPT 180

Query: 181 VGVGGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSF 240
           VGVGG FSGGGYG+LLRKYGLA DNV+DAYL+DA+GKF++RESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVA 245
           GIVVA
Sbjct: 241 GIVVA 245

BLAST of MS026400 vs. ExPASy TrEMBL
Match: A0A6J1CKW8 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.4e-105
Identity = 194/242 (80.17%), Postives = 215/242 (88.84%), Query Frame = 0

Query: 3   YSSPLIPLALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSSYS 62
           YSSPLIPL LAF  LSSS  W  A ADK+E+FLQCLLNHSPD N+SISKV++TPINSSYS
Sbjct: 5   YSSPLIPLFLAFIFLSSSRFWTLAVADKHESFLQCLLNHSPDGNYSISKVIHTPINSSYS 64

Query: 63  SILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGLSY 122
           S+L+FSIRNLRFST ETPKPLLIITPSHVSHIQAAV+CSK +G QIRTRSGGHDFE LSY
Sbjct: 65  SVLDFSIRNLRFSTAETPKPLLIITPSHVSHIQAAVVCSKRYGFQIRTRSGGHDFERLSY 124

Query: 123 VAHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTMVGV 182
           VAHL FIIVDL NL+SIS+D+K+N AW+ SGAT+GELY SIA+K+ T AF A V   VGV
Sbjct: 125 VAHLSFIIVDLINLRSISVDVKNNNAWVHSGATLGELYYSIAQKSRTSAFPAGVCPTVGV 184

Query: 183 GGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 242
           GG FSGGGYG+LLRKYGLA DNV+DAYL+DANGKF+DRESMGEDLFWAIRGGGGGSFGIV
Sbjct: 185 GGHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIV 244

Query: 243 VA 245
           VA
Sbjct: 245 VA 246

BLAST of MS026400 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 6.8e-105
Identity = 191/246 (77.64%), Postives = 217/246 (88.21%), Query Frame = 0

Query: 1   MKYSSPLIPL--ALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPIN 60
           MKYSSPLIPL  +L F  LSSSS W  AS DK+E+FL CLL+HSPDSN+SISKVVYTP+N
Sbjct: 1   MKYSSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLN 60

Query: 61  SSYSSILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFE 120
           SSYSS+L+FSIRNLRFST +TPKPL IITP HVSH+QAAV+CS+THGLQIRTRSGGHDFE
Sbjct: 61  SSYSSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFE 120

Query: 121 GLSYVAHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVST 180
           GLS V+HLPFII+DL NL+SI+ID  +NTAW+QSGAT+GELY SIAEK+ TLAF A    
Sbjct: 121 GLSCVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACP 180

Query: 181 MVGVGGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGS 240
            VG+GG FSGGGYG++LRK+GLA DNV+DAYL+DANGKF DRE MGEDLFWAIRGGGGGS
Sbjct: 181 TVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGS 240

Query: 241 FGIVVA 245
           FGIVVA
Sbjct: 241 FGIVVA 246

BLAST of MS026400 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 2.0e-104
Identity = 196/244 (80.33%), Postives = 221/244 (90.57%), Query Frame = 0

Query: 1   MKYSSPLIPLALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSS 60
           MKY SPL  L+LAF LLSSSSSW  ASA+K+EAFLQCL +HS DS+ SISKV+YTPIN S
Sbjct: 1   MKY-SPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSS-SISKVIYTPINPS 60

Query: 61  YSSILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGL 120
           YSS+LNFS+RNLRFS+ ETPKPLLIITPS+VSH+QAAVICSKTHGLQIR RSGGHD EGL
Sbjct: 61  YSSVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGL 120

Query: 121 SYVAHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTMV 180
           SYVAHLPFII+DL NLKSI+++I+++TAWIQSGAT+GELY +IA+K+ TLAF A V   V
Sbjct: 121 SYVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTV 180

Query: 181 GVGGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFG 240
           GVGG FSGGGYG++LRKYGLA DNV+DAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVA 245
           IVVA
Sbjct: 241 IVVA 242

BLAST of MS026400 vs. ExPASy TrEMBL
Match: A0A5D3C1A1 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00110 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.8e-98
Identity = 185/244 (75.82%), Postives = 209/244 (85.66%), Query Frame = 0

Query: 1   MKYSSPLIPLALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSS 60
           MKYSSPLIP +L   L+SSSSSWV AS  K++ FLQCL  HS    + ISKVV+TPINSS
Sbjct: 1   MKYSSPLIPFSLILILVSSSSSWVSASPHKHQNFLQCLSEHS-SKRYPISKVVHTPINSS 60

Query: 61  YSSILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGL 120
           YSS+L+FSIRNL FS  ETPKPLLIITPSHVSHIQAAVICSK+HGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTMV 180
           SYVA+ PFIIVDL NL+SI ID++ NTAW++SGAT+GELY  I EK+ TLAF   V   V
Sbjct: 121 SYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTV 180

Query: 181 GVGGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFG 240
           GVGG FSGGGYG++LRK+GLA DNV+DAYL+DANGK +DR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVA 245
           IVVA
Sbjct: 241 IVVA 243

BLAST of MS026400 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 262.3 bits (669), Expect = 4.1e-70
Identity = 133/246 (54.07%), Postives = 177/246 (71.95%), Query Frame = 0

Query: 3   YSSPLIPLALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSSYS 62
           +SS LI    +  LLS     V A+     +FLQCL     DSN  +SKV++TP ++S+S
Sbjct: 7   FSSVLIFFTTSTLLLSIPHP-VSANRSNQSSFLQCLSLQLNDSNI-VSKVIHTPNDTSFS 66

Query: 63  SILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGLSY 122
           S+L  SI+N RFS  + PKP+LI+TP   S +Q+AV C++  G+ IRTRSGGHD+EGLSY
Sbjct: 67  SVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSY 126

Query: 123 VAHLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTMVGV 182
           V H PF+I+DLRNL+SI++D+ + + W+Q+GAT+GELY  I +K  TLAF A V   VGV
Sbjct: 127 VTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGV 186

Query: 183 GGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 242
           GG FSGGGYG LLRK+GLA D+V+DA ++DA G+  +R  MGED FWAIRGGGG SF +V
Sbjct: 187 GGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVV 246

Query: 243 VACEAG 249
           ++ + G
Sbjct: 247 LSWKIG 250

BLAST of MS026400 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 255.8 bits (652), Expect = 3.8e-68
Identity = 127/242 (52.48%), Postives = 175/242 (72.31%), Query Frame = 0

Query: 7   LIPLALAFFLLSSSSSW---VGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSSYSS 66
           L+ L + F LL+ S S    + A    +E FL+CL   S   N   S++++T  + SY S
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCL---SHRINEDDSRIIHTSKDPSYFS 66

Query: 67  ILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGLSYV 126
           ILN SI+N RF  +ETPKP+ IITP   + +Q+ + C++ HG+ IRTRSGGHD+EGLSY+
Sbjct: 67  ILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYM 126

Query: 127 A-HLPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTMVGV 186
           A   PF+++DLRNL+SI++D+ + T W+QSGAT+GELY  I + + +LAF A +   VG+
Sbjct: 127 AKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGI 186

Query: 187 GGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 245
           GGQF GGGYG L+RKYGL+ DNV+DA+++DANG F DR+ MGED FWAIRGGGG SF +V
Sbjct: 187 GGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVV 245

BLAST of MS026400 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 253.8 bits (647), Expect = 1.5e-67
Identity = 127/246 (51.63%), Postives = 169/246 (68.70%), Query Frame = 0

Query: 1   MKYSSPLIPLALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSS 60
           MKY+  L+   + F   SSSSS         E F QCL ++S D    IS  ++   N S
Sbjct: 1   MKYALILVLFFVVFIWQSSSSS------ANSETFTQCLTSNS-DPKHPISPAIFFSGNGS 60

Query: 61  YSSILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGL 120
           YSS+L  +IRNLRF+T  TPKP LII  +H SH+QAA+ C K H LQ++ RSGGHD++GL
Sbjct: 61  YSSVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGL 120

Query: 121 SYVAH--LPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVST 180
           SYV +   PF ++D+ NL+S+ +D+   TAW+Q+GA +GE+Y  I EK+ TLA+ A +  
Sbjct: 121 SYVTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICP 180

Query: 181 MVGVGGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGS 240
            VGVGG  SGGGYG ++RKYGL  DN +DA ++D NGK  DR+ MGEDL+WAI GGGGGS
Sbjct: 181 TVGVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGS 239

Query: 241 FGIVVA 245
           +G+V+A
Sbjct: 241 YGVVLA 239

BLAST of MS026400 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 251.9 bits (642), Expect = 5.5e-67
Identity = 129/245 (52.65%), Postives = 177/245 (72.24%), Query Frame = 0

Query: 1   MKYSSPLIPLALAFFLLSSSSSWVGASADKYEAFLQCLLNHSPDSNFSISKVVYTPINSS 60
           M++SS L  + L  F L + S  V A    +E FL+C L++  + N    KV++T  +SS
Sbjct: 1   MEFSSFLFTILL--FSL-NISPLVSAHGSNHEDFLKC-LSYRMNDNTVEPKVIHTSKDSS 60

Query: 61  YSSILNFSIRNLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGL 120
           + SIL+ SI+N RFS  ETPKP+ IITP   S +Q  + C++ HG+ +RTRS GH +EGL
Sbjct: 61  FFSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGL 120

Query: 121 SYVAH-LPFIIVDLRNLKSISIDIKDNTAWIQSGATVGELYNSIAEKTPTLAFAASVSTM 180
           SY+A+  PF ++DLRNL+SIS+D+ + T W+Q+GAT GELY  I + T +LAF A +   
Sbjct: 121 SYIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPT 180

Query: 181 VGVGGQFSGGGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSF 240
           VGVGGQFSGGGYG LLRKYGLA DN++DA ++DA+G+  DR++MGED FWAIRGGGG SF
Sbjct: 181 VGVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSF 240

Query: 241 GIVVA 245
           G++++
Sbjct: 241 GVILS 241

BLAST of MS026400 vs. TAIR 10
Match: AT1G30740.1 (FAD-binding Berberine family protein )

HSP 1 Score: 240.0 bits (611), Expect = 2.2e-63
Identity = 125/236 (52.97%), Postives = 166/236 (70.34%), Query Frame = 0

Query: 12  LAFFLLSSSSSWVGA-SADKYEAFLQCLLNHSPDSNFSISKVVYTPINSSYSSILNFSIR 71
           L FF  S S S   A S   YE F+QC  N +  S+  +S VV    + S++  L   IR
Sbjct: 6   LLFFAASYSMSLSSADSVTIYEDFVQCFKNVTTISDIDLSDVVLPRTSISFTPTLRAYIR 65

Query: 72  NLRFSTVETPKPLLIITPSHVSHIQAAVICSKTHGLQIRTRSGGHDFEGLSYVAHLPFII 131
           N RF+T   PKP +II P   SH+QAAVIC+KT  LQ++ RSGGHD++GLSYV+ + F++
Sbjct: 66  NARFNTSSMPKPSIIIVPRVDSHVQAAVICAKTLNLQLKIRSGGHDYDGLSYVSAVTFLV 125

Query: 132 VDLRNLKSISIDIKD--NTAWIQSGATVGELYNSIAEKTPTLAFAASVSTMVGVGGQFSG 191
           +DL N ++I++D+ D   +AW+Q+GAT+GELY  I EK+   AF A V   VGVGG  SG
Sbjct: 126 LDLSNFRNITVDLNDGGGSAWVQTGATLGELYYRIWEKSEVHAFPAGVCPTVGVGGHVSG 185

Query: 192 GGYGMLLRKYGLATDNVVDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVA 245
           GGYG ++RK+GL  D+VVDA ++DANG+ +DR+SM EDLFWAIRGGGGGSFG+V+A
Sbjct: 186 GGYGHMIRKFGLTIDHVVDATIVDANGQIHDRKSMEEDLFWAIRGGGGGSFGVVLA 241