MS026369 (gene) Bitter gourd (TR) v1

Overview
NameMS026369
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionATP-dependent 6-phosphofructokinase
Locationscaffold459: 807295 .. 810444 (+)
RNA-Seq ExpressionMS026369
SyntenyMS026369
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGCTTCTTCTACTTCAATCTCCTCCTCTTTCCTTTCCCTTTTCTCTCCCAGATTCAGCGGCATCTGCTGGAACTGGTTCGACAAATCTACACCCTCTAATCACCGTCGTTGTGGATCGTCTTCGTCGTCGACAATGGACGATCACGATCATTCCAAGAAAGAAATCTTCTCGGGCGACTTCCGATATGTTCTGGAAGATGTCCCTCACCTGATCGATTATCTCCCTAACCTCTCGGTAACTATCACTCTCCCTATTTGTTGGCAGAGAAAGCACAGTAAAAAGAGAGAAAAAAAAAAAGATAGCAGTGATGAAATAAAATTCGTCAGTTTATATTACGGTTGATTAAAAAAAAAAAAAAAACCTAACAAAACCAACTAATCGTATTCGTACGTTGCCCTGTCTTAAATTGAGAGTTTTATCGATAAATTTAAATATCTAATAGATTTTTAAACTTTTAATTCTTGAATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTGACAAAATGGTAATAGTAATTTCGTGATAAAATGAGNAAATTCATTTACTTATTTTCATCATAGGTTTAAAATGTAACTTTTATTTTGTTTTGTTTACAGGCATATCCAAATCCCCTGCTGAACAATAATTCTGCCTATTCTGCTACTGAGTATGTCTCATTATTTTGGCTAATGCACATTTTATTCTCGTTTGGAAATAATATTGATTATCATTCAAATACTAATGAAAAAGTGATGTAACCTTTGATTCAGGCAATATTTTGTTAACATGGATGATGTTGTTGCTCAAAAGGTAAGTAATTTGTTTGAGTTCAAATTTAAATTTAAATTCAACTTCATTGTTATTGTTATATATTGAACCGTTCTGATTTCTGTGGAGTTTCATTTATTCCGTTCAGATTGTTGTTGAGAAGAACTCTCTAAGTGGAGCATATTTCCGACTTGCTGGACCTCGCCAAAAGGTTCACTAAATGTTCATTTTCTTTTCTTCTTCTACCTTTTTCCCCTGCAATTACTTTGACATCGTCATTTTAACTATTTTAGATATTAATTAACACATCTAATCTTTTTCAGGTGTTTTTTGAACCCGAAGACGTTCGTGCATGTATTGTAACCTGTGGTGGCTTGTGCCCAGGTCTTAACACAGTGATTAGAGAAATAGTATGTGGATTAAACCACATGTATGGCGTCCACAAAATTGTTGGAATTGAGGTAATTACATACTCTTTAATTATTATTCTTAATATCATTGCTACACATAGGCATGTTTGAAAGTGATTTTTAAACAAGTAATTTTACCAAAAGTGATTTAACTATAAGTGATTTTGAAAGGACGTGAAAAAAAAAATCACATTCCAAATGATTAAACTAGATCCCTTTCATGCAAACATACCCAACAAATTGACATAATCATGCATAAAATTGATTATTTGACTCAATACAGGGAGGATACAAAGGTTTCTACGGTGAGAATACAATTTCATTAACACCAAAATCAGTGAATAATATCCACAAACGTGGTGGCACAATTCTTGGAACATCAAGAGGTGGTCACAATACGTCCAACATTGTTGACAGCATTCAAGATAGAGGCATCAATCAGGTGAAAAATCTGATAAAAAGTTAAAAATTTTCATGATATCTTAACTTGGAAGTCATATGTAAAATTTGTAAAGTATGGTTTTTTCTAGTTTCTTAAATTTGCATTCTGGTTTTCAGGTCTATATACTTGGAGGGGATGGAACTCAAAAAGGAGCTGCTGCTATTTATGAGGTAATGGAAACTGTTTCTAAAACTATGGGTATGAATAATGCCTAAAGATTGAAGAGATGTTGAAATCTTATATGAATTATGTTTCTCATTTCTCTAGGAAATTAGAAGGCGTGGCCTCAAAGTTGCAGTGGTTGGAATTCCAAAGACTATTGATAATGATATTCCGGTAAATACAATTAAAATTTTATTTGTTGATAATTTATGAGTTATGCATTAACAATCAAATCTATATTTGAAAATTTTCACTGAGAAGCATCGTGTTCATCTTCTTTCAGGTAATAGACAAGTCATTTGGTTTTGACACTGCCGTTGAAGAAGCTCAACGTGCTATTGATGCTGCTCATGTTGAAGCAGAGAGTAACGAGAATGGTATAGGCTTGGTAAAGTTGATGGGTCGCAACAGTGGTAAGTTTCATCATTTCGTAGCTAGTTCTGTATGAGAATTTTCAACTCATAATACATATTTTTTTAACCAATATATATTTCTCAATATCAAATCACATAGGGTTCATAGCAATGTATGCGTGCCTTGCCAGTCGAGACGTTGACTGCTGCCTGATACCAGAGTCGCCCTTCTATCTCGAAGGAGAGGGTGGTCTTTTCGAGTACATTGAACATTGCTTGGAAAAACAAGGCCATATGGTTATAGTTATAGCAGAAGGTGCCGGTCAAGAACTTGTTTCCAAAAGCACACAAAAAGCCGATCAACTAGATGCTTCTGGAAATAAGCTACACCAAGATGTTGGCTCGTGGATATCCCAACAGATAAAAGTTTGTCCTCTCCCCTCAACTTTACGTAAAATATTTCACTACTTTGAAAATACTTTAACTAAAAATGTTCTTGTTTGATCTAGAATCATTTCGAAGAAAAAGGGACCATTCCAATCAATCTCAAATATATAGGTTTAACTTTCTCTCCATTGCTAAAATTTTCTACTTTAACACATGTAATAGCATGTAACGTTTTTGTTTATCGTCTTGGCAGATCCTACGTATATGATTCGTGCAGTTCCTAGCATTTCATCAGACAACATCTTCTGTACCCTTCTTGCTCATAGTGCCATTCATGGCGCAATGGCAGGATACACTGGTTTCACAGTAGGCCCGGTGAATGGACAACATGTTTACATACCATTTCACGTAAGTCTAAAATCAAAATCTTTTGAAAATTTGTCAAGCAGCATTATGAAATGAGGTTCGTCACTGTTTGAATGTAGACCAAATTTATGTTGCTTTTATATGTGCAGCGGATAATCGAGAGACAGAACAAAGTTGCTATTACTGATCGGATGTGGGCGAGGCTATTGTGTTCTACCAACCAGCCAAGTTTCTTGGATCCTAAACACATCACTAAAGCTTCTTACTAG

mRNA sequence

ATGGACGCTTCTTCTACTTCAATCTCCTCCTCTTTCCTTTCCCTTTTCTCTCCCAGATTCAGCGGCATCTGCTGGAACTGGTTCGACAAATCTACACCCTCTAATCACCGTCGTTGTGGATCGTCTTCGTCGTCGACAATGGACGATCACGATCATTCCAAGAAAGAAATCTTCTCGGGCGACTTCCGATATGTTCTGGAAGATGTCCCTCACCTGATCGATTATCTCCCTAACCTCTCGGCATATCCAAATCCCCTGCTGAACAATAATTCTGCCTATTCTGCTACTGAGCAATATTTTGTTAACATGGATGATGTTGTTGCTCAAAAGATTGTTGTTGAGAAGAACTCTCTAAGTGGAGCATATTTCCGACTTGCTGGACCTCGCCAAAAGGTGTTTTTTGAACCCGAAGACGTTCGTGCATGTATTGTAACCTGTGGTGGCTTGTGCCCAGGTCTTAACACAGTGATTAGAGAAATAGTATGTGGATTAAACCACATGTATGGCGTCCACAAAATTGTTGGAATTGAGGGAGGATACAAAGGTTTCTACGGTGAGAATACAATTTCATTAACACCAAAATCAGTGAATAATATCCACAAACGTGGTGGCACAATTCTTGGAACATCAAGAGGTGGTCACAATACGTCCAACATTGTTGACAGCATTCAAGATAGAGGCATCAATCAGGTCTATATACTTGGAGGGGATGGAACTCAAAAAGGAGCTGCTGCTATTTATGAGGAAATTAGAAGGCGTGGCCTCAAAGTTGCAGTGGTTGGAATTCCAAAGACTATTGATAATGATATTCCGGTAATAGACAAGTCATTTGGTTTTGACACTGCCGTTGAAGAAGCTCAACGTGCTATTGATGCTGCTCATGTTGAAGCAGAGAGTAACGAGAATGGTATAGGCTTGGTAAAGTTGATGGGTCGCAACAGTGGGTTCATAGCAATGTATGCGTGCCTTGCCAGTCGAGACGTTGACTGCTGCCTGATACCAGAGTCGCCCTTCTATCTCGAAGGAGAGGGTGGTCTTTTCGAGTACATTGAACATTGCTTGGAAAAACAAGGCCATATGGTTATAGTTATAGCAGAAGATCCTACGTATATGATTCGTGCAGTTCCTAGCATTTCATCAGACAACATCTTCTGTACCCTTCTTGCTCATAGTGCCATTCATGGCGCAATGGCAGGATACACTGGTTTCACAGTAGGCCCGGTGAATGGACAACATGTTTACATACCATTTCACCGGATAATCGAGAGACAGAACAAAGTTGCTATTACTGATCGGATGTGGGCGAGGCTATTGTGTTCTACCAACCAGCCAAGTTTCTTGGATCCTAAACACATCACTAAAGCTTCTTACTAG

Coding sequence (CDS)

ATGGACGCTTCTTCTACTTCAATCTCCTCCTCTTTCCTTTCCCTTTTCTCTCCCAGATTCAGCGGCATCTGCTGGAACTGGTTCGACAAATCTACACCCTCTAATCACCGTCGTTGTGGATCGTCTTCGTCGTCGACAATGGACGATCACGATCATTCCAAGAAAGAAATCTTCTCGGGCGACTTCCGATATGTTCTGGAAGATGTCCCTCACCTGATCGATTATCTCCCTAACCTCTCGGCATATCCAAATCCCCTGCTGAACAATAATTCTGCCTATTCTGCTACTGAGCAATATTTTGTTAACATGGATGATGTTGTTGCTCAAAAGATTGTTGTTGAGAAGAACTCTCTAAGTGGAGCATATTTCCGACTTGCTGGACCTCGCCAAAAGGTGTTTTTTGAACCCGAAGACGTTCGTGCATGTATTGTAACCTGTGGTGGCTTGTGCCCAGGTCTTAACACAGTGATTAGAGAAATAGTATGTGGATTAAACCACATGTATGGCGTCCACAAAATTGTTGGAATTGAGGGAGGATACAAAGGTTTCTACGGTGAGAATACAATTTCATTAACACCAAAATCAGTGAATAATATCCACAAACGTGGTGGCACAATTCTTGGAACATCAAGAGGTGGTCACAATACGTCCAACATTGTTGACAGCATTCAAGATAGAGGCATCAATCAGGTCTATATACTTGGAGGGGATGGAACTCAAAAAGGAGCTGCTGCTATTTATGAGGAAATTAGAAGGCGTGGCCTCAAAGTTGCAGTGGTTGGAATTCCAAAGACTATTGATAATGATATTCCGGTAATAGACAAGTCATTTGGTTTTGACACTGCCGTTGAAGAAGCTCAACGTGCTATTGATGCTGCTCATGTTGAAGCAGAGAGTAACGAGAATGGTATAGGCTTGGTAAAGTTGATGGGTCGCAACAGTGGGTTCATAGCAATGTATGCGTGCCTTGCCAGTCGAGACGTTGACTGCTGCCTGATACCAGAGTCGCCCTTCTATCTCGAAGGAGAGGGTGGTCTTTTCGAGTACATTGAACATTGCTTGGAAAAACAAGGCCATATGGTTATAGTTATAGCAGAAGATCCTACGTATATGATTCGTGCAGTTCCTAGCATTTCATCAGACAACATCTTCTGTACCCTTCTTGCTCATAGTGCCATTCATGGCGCAATGGCAGGATACACTGGTTTCACAGTAGGCCCGGTGAATGGACAACATGTTTACATACCATTTCACCGGATAATCGAGAGACAGAACAAAGTTGCTATTACTGATCGGATGTGGGCGAGGCTATTGTGTTCTACCAACCAGCCAAGTTTCTTGGATCCTAAACACATCACTAAAGCTTCTTACTAG

Protein sequence

MDASSTSISSSFLSLFSPRFSGICWNWFDKSTPSNHRRCGSSSSSTMDDHDHSKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIVVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHKIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVYILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDAAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLEKQGHMVIVIAEDPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRIIERQNKVAITDRMWARLLCSTNQPSFLDPKHITKASY
Homology
BLAST of MS026369 vs. NCBI nr
Match: XP_022153587.1 (ATP-dependent 6-phosphofructokinase 3-like [Momordica charantia])

HSP 1 Score: 815.5 bits (2105), Expect = 2.4e-232
Identity = 410/464 (88.36%), Postives = 410/464 (88.36%), Query Frame = 0

Query: 47  MDDHDHSKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDV 106
           MDDHDHSKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDV
Sbjct: 1   MDDHDHSKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDV 60

Query: 107 VAQKIVVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNH 166
           VAQKIVVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIV GLNH
Sbjct: 61  VAQKIVVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVGGLNH 120

Query: 167 MYGVHKIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDR 226
           MYGVHKIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDR
Sbjct: 121 MYGVHKIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDR 180

Query: 227 GINQVYILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEA 286
           GINQVYILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEA
Sbjct: 181 GINQVYILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEA 240

Query: 287 QRAIDAAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGL 346
           QRAIDAAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGL
Sbjct: 241 QRAIDAAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGL 300

Query: 347 FEYIEHCLEKQGHMVIVIAE---------------------------------------- 406
           FEYIEHCLEKQGHMVIVIAE                                        
Sbjct: 301 FEYIEHCLEKQGHMVIVIAEGAGQELVSKSTQKADQLDASGNKLHQDVGSWISQQIKNHF 360

Query: 407 -------------DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHV 458
                        DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHV
Sbjct: 361 EEKGTIPINLKYIDPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHV 420

BLAST of MS026369 vs. NCBI nr
Match: XP_022153589.1 (ATP-dependent 6-phosphofructokinase 6-like isoform X2 [Momordica charantia])

HSP 1 Score: 692.6 bits (1786), Expect = 2.3e-195
Identity = 361/506 (71.34%), Postives = 390/506 (77.08%), Query Frame = 0

Query: 1   MDASSTSISSSFLSLFSPRFSGICWNWFDKSTPSNHRRCGSSSSSTMDDHDHSKKEIFSG 60
           MD+S +S SSS  S F   FS           P +HRR  +  SS     DH+ ++   G
Sbjct: 1   MDSSVSSFSSSSTS-FDFNFSA--------PGPDHHRRFQNGESSPSSAMDHNGRDA-PG 60

Query: 61  DFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIVVEKNSLSG 120
           DFRYVLEDVPHL DYLP+L AYPNP L +N AYS  +QYFV++DD VAQ +VV KN+  G
Sbjct: 61  DFRYVLEDVPHLTDYLPDLPAYPNP-LQDNPAYSVVKQYFVDVDDAVAQSVVVHKNTPRG 120

Query: 121 AYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHKIVGIEGGY 180
            +FR  GPRQKVFFE ++V ACIVTCGGLCPGLNTVIREIVCGL HMYGVHK+VGIEGGY
Sbjct: 121 THFRRTGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVHKVVGIEGGY 180

Query: 181 KGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVYILGGDGTQ 240
           KGFY  NTISLTPKSVNNIHKRGGTILGTSRGGHNT+ IVDSIQ RGINQVYILGGDGTQ
Sbjct: 181 KGFYARNTISLTPKSVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQVYILGGDGTQ 240

Query: 241 KGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDAAHVEAESN 300
           KGAAAI+EEIRRRGLKVAV GIPKTIDNDIPVIDKSFGFDTAVEEAQRAI+AAHVE+ESN
Sbjct: 241 KGAAAIFEEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVESESN 300

Query: 301 ENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLEKQGHM 360
           EN IGLVKLMGRNSGFIAMYA LASRDVDCCLIPESPFYLEGEGGLFEYIEHCL++QGHM
Sbjct: 301 ENCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLKEQGHM 360

Query: 361 VIVIAE-----------------------------------------------------D 420
           VIVIAE                                                     D
Sbjct: 361 VIVIAEGAGQELISKSMHNMDNQDASGNKLHQDVGLWISQRIKDYFKEMRKIAIDLKYID 420

Query: 421 PTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRIIERQNKV 454
           PTYMIRAVPSI+SDNIFC+LLA SAIHGAMAGYTGFTVGPVNG+H YIPFHRIIERQNKV
Sbjct: 421 PTYMIRAVPSIASDNIFCSLLAQSAIHGAMAGYTGFTVGPVNGRHAYIPFHRIIERQNKV 480

BLAST of MS026369 vs. NCBI nr
Match: XP_022153588.1 (ATP-dependent 6-phosphofructokinase 6-like isoform X1 [Momordica charantia])

HSP 1 Score: 679.1 bits (1751), Expect = 2.7e-191
Identity = 361/531 (67.98%), Postives = 391/531 (73.63%), Query Frame = 0

Query: 1   MDASSTSISSSFLSLFSPRFSGICWNWFDKSTPSNHRRCGSSSSSTMDDHDHSKKEIFSG 60
           MD+S +S SSS  S F   FS           P +HRR  +  SS     DH+ ++   G
Sbjct: 1   MDSSVSSFSSSSTS-FDFNFSA--------PGPDHHRRFQNGESSPSSAMDHNGRDA-PG 60

Query: 61  DFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQ----------- 120
           DFRYVLEDVPHL DYLP+L AYPNP L +N AYS  +QYFV++DD VAQ           
Sbjct: 61  DFRYVLEDVPHLTDYLPDLPAYPNP-LQDNPAYSVVKQYFVDVDDAVAQSVSNLCLNSKV 120

Query: 121 --------------KIVVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNT 180
                         ++VV KN+  G +FR  GPRQKVFFE ++V ACIVTCGGLCPGLNT
Sbjct: 121 LDGIVLNFVGFCSKQVVVHKNTPRGTHFRRTGPRQKVFFESDEVHACIVTCGGLCPGLNT 180

Query: 181 VIREIVCGLNHMYGVHKIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHN 240
           VIREIVCGL HMYGVHK+VGIEGGYKGFY  NTISLTPKSVNNIHKRGGTILGTSRGGHN
Sbjct: 181 VIREIVCGLYHMYGVHKVVGIEGGYKGFYARNTISLTPKSVNNIHKRGGTILGTSRGGHN 240

Query: 241 TSNIVDSIQDRGINQVYILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDK 300
           T+ IVDSIQ RGINQVYILGGDGTQKGAAAI+EEIRRRGLKVAV GIPKTIDNDIPVIDK
Sbjct: 241 TTKIVDSIQHRGINQVYILGGDGTQKGAAAIFEEIRRRGLKVAVAGIPKTIDNDIPVIDK 300

Query: 301 SFGFDTAVEEAQRAIDAAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPE 360
           SFGFDTAVEEAQRAI+AAHVE+ESNEN IGLVKLMGRNSGFIAMYA LASRDVDCCLIPE
Sbjct: 301 SFGFDTAVEEAQRAINAAHVESESNENCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPE 360

Query: 361 SPFYLEGEGGLFEYIEHCLEKQGHMVIVIAE----------------------------- 420
           SPFYLEGEGGLFEYIEHCL++QGHMVIVIAE                             
Sbjct: 361 SPFYLEGEGGLFEYIEHCLKEQGHMVIVIAEGAGQELISKSMHNMDNQDASGNKLHQDVG 420

Query: 421 ------------------------DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTG 454
                                   DPTYMIRAVPSI+SDNIFC+LLA SAIHGAMAGYTG
Sbjct: 421 LWISQRIKDYFKEMRKIAIDLKYIDPTYMIRAVPSIASDNIFCSLLAQSAIHGAMAGYTG 480

BLAST of MS026369 vs. NCBI nr
Match: KAA0050858.1 (ATP-dependent 6-phosphofructokinase 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 674.1 bits (1738), Expect = 8.5e-190
Identity = 338/447 (75.62%), Postives = 360/447 (80.54%), Query Frame = 0

Query: 60  GDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIVVEKNSLS 119
           GD+ YVLEDVPHL DYLP+L A  NP L ++ AYSA +QYFVN+DD VAQ +VV  NS  
Sbjct: 19  GDYHYVLEDVPHLTDYLPDLPANLNP-LQDHPAYSAVKQYFVNVDDAVAQNVVVHNNSPR 78

Query: 120 GAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHKIVGIEGG 179
           G +FR AGPRQKVFFE ++V ACIVTCGGLCPGLNTVIREIVCGL HMYGVHK+VGIEGG
Sbjct: 79  GTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVHKVVGIEGG 138

Query: 180 YKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVYILGGDGT 239
           Y+GFY  NTI LTPKSVNNIHKRGGTILGTSRGGHNT+ IVDSIQ RGINQVYILGGDGT
Sbjct: 139 YRGFYARNTIPLTPKSVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQVYILGGDGT 198

Query: 240 QKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDAAHVEAES 299
           QKGAAAI+EEIRRRGLKVAV GIPKTIDNDIPVIDKSFGFDTAVEEAQRAI AAHVE+ES
Sbjct: 199 QKGAAAIFEEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESES 258

Query: 300 NENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLEKQGH 359
           NENGIGLVKLMGRNSGFIAMYA LASRDVDCCLIPESPFYLEGEGGLFEYIEHC+++ GH
Sbjct: 259 NENGIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIKEHGH 318

Query: 360 MVIVIAE----------------------------------------------------- 419
           MVIVIAE                                                     
Sbjct: 319 MVIVIAEGAGQELMSESIHKLDQQDASGNKLHQDVGLWISQQIKDFFKEKRKMVINLKYI 378

Query: 420 DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRIIERQNK 454
           DPTYMIRAVPSI+SDNIFCTLLA SAIHGAMAGYTGFTVGPVNG+H YIPFHRIIERQNK
Sbjct: 379 DPTYMIRAVPSIASDNIFCTLLAQSAIHGAMAGYTGFTVGPVNGRHAYIPFHRIIERQNK 438

BLAST of MS026369 vs. NCBI nr
Match: TYK08494.1 (ATP-dependent 6-phosphofructokinase 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 674.1 bits (1738), Expect = 8.5e-190
Identity = 338/447 (75.62%), Postives = 360/447 (80.54%), Query Frame = 0

Query: 60  GDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIVVEKNSLS 119
           GD+ YVLEDVPHL DYLP+L A  NP L ++ AYSA +QYFVN+DD VAQ +VV  NS  
Sbjct: 19  GDYHYVLEDVPHLTDYLPDLPANLNP-LQDHPAYSAVKQYFVNVDDAVAQNVVVHNNSPR 78

Query: 120 GAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHKIVGIEGG 179
           G +FR AGPRQKVFFE ++V ACIVTCGGLCPGLNTVIREIVCGL HMYGVHK+VGIEGG
Sbjct: 79  GTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVHKVVGIEGG 138

Query: 180 YKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVYILGGDGT 239
           Y+GFY  NTI LTPKSVNNIHKRGGTILGTSRGGHNT+ IVDSIQ RGINQVYILGGDGT
Sbjct: 139 YRGFYARNTIPLTPKSVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQVYILGGDGT 198

Query: 240 QKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDAAHVEAES 299
           QKGAAAI+EEIRRRGLKVAV GIPKTIDNDIPVIDKSFGFDTAVEEAQRAI AAHVE+ES
Sbjct: 199 QKGAAAIFEEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESES 258

Query: 300 NENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLEKQGH 359
           NENGIGLVKLMGRNSGFIAMYA LASRDVDCCLIPESPFYLEGEGGLFEYIEHC+++ GH
Sbjct: 259 NENGIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIKEHGH 318

Query: 360 MVIVIAE----------------------------------------------------- 419
           MVIVIAE                                                     
Sbjct: 319 MVIVIAEGAGQELMSESIHKLDQQDASGNKLHQDVGLWISQQIKDFFKEKRKMVINLKYI 378

Query: 420 DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRIIERQNK 454
           DPTYMIRAVPSI+SDNIFCTLLA SAIHGAMAGYTGFTVGPVNG+H YIPFHRIIERQNK
Sbjct: 379 DPTYMIRAVPSIASDNIFCTLLAQSAIHGAMAGYTGFTVGPVNGRHAYIPFHRIIERQNK 438

BLAST of MS026369 vs. ExPASy Swiss-Prot
Match: Q94AA4 (ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE=1 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 2.9e-172
Identity = 308/458 (67.25%), Postives = 349/458 (76.20%), Query Frame = 0

Query: 47  MDDHDHSKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDV 106
           M   + SK +I +G   YVLEDVPHL DYLP L  YPNP L +N AYS  +QYFV+ DD 
Sbjct: 1   MSTVESSKPKIINGSCGYVLEDVPHLSDYLPGLPTYPNP-LQDNPAYSVVKQYFVDADDS 60

Query: 107 VAQKIVVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNH 166
           V QKIVV K+   G +FR AGPRQKV+FE ++V ACIVTCGGLCPGLNTVIREIV  L++
Sbjct: 61  VPQKIVVHKDGPRGIHFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSY 120

Query: 167 MYGVHKIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDR 226
           MYGV +I+GI+GGY+GFY +NT+SL  K VN+IHKRGGTILGTSRGGH+T+ IVDSIQDR
Sbjct: 121 MYGVKRILGIDGGYRGFYAKNTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDR 180

Query: 227 GINQVYILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEA 286
           GINQVYI+GGDGTQ+GA+ I+EEIRRRGLKVAV+GIPKTIDNDIPVIDKSFGFDTAVEEA
Sbjct: 181 GINQVYIIGGDGTQRGASVIFEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEA 240

Query: 287 QRAIDAAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGL 346
           QRAI+AAHVEAES ENGIG+VKLMGR SGFIAMYA LASRDVDCCLIPESPFYLEGEGGL
Sbjct: 241 QRAINAAHVEAESIENGIGVVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGL 300

Query: 347 FEYIEHCLEKQGHMVIVIAE---------------------------------------- 406
           FEYIE  L++ GHMV+VIAE                                        
Sbjct: 301 FEYIEKRLKESGHMVLVIAEGAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHF 360

Query: 407 ------------DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVY 453
                       DPTYMIRAVPS +SDN++CTLLA SA+HGAMAGYTG+  G VNG+  Y
Sbjct: 361 NQKKMVMNLKYIDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTY 420

BLAST of MS026369 vs. ExPASy Swiss-Prot
Match: Q9C5J7 (ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE=1 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.1e-170
Identity = 301/449 (67.04%), Postives = 345/449 (76.84%), Query Frame = 0

Query: 53  SKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIV 112
           +K +I +G   Y+L+DVPHLIDYLP+L  YPNP L +N AYS  +QYFV+ DD V +K+V
Sbjct: 7   NKPKIVNGPGGYILQDVPHLIDYLPDLPTYPNP-LQDNPAYSVVKQYFVHADDSVPEKVV 66

Query: 113 VEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHK 172
           V K+   G +FR AGPRQKV+FE ++V ACIVTCGGLCPGLNTVIRE+V  L++MYGV +
Sbjct: 67  VHKDGPRGVHFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKR 126

Query: 173 IVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVY 232
           I+GI+GGY+GFY +NTI L  K VN+IHKRGGTI+GTSRGGH+T+ IVDSIQDRGINQVY
Sbjct: 127 ILGIDGGYRGFYAKNTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVY 186

Query: 233 ILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDA 292
           I+GGDGTQ+GA+ I+EEIRRR LKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAI+A
Sbjct: 187 IIGGDGTQRGASVIFEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINA 246

Query: 293 AHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEH 352
           AHVEAESNENGIG VKLMGR SG+IAMYA LASRDVDCCLIPESPFYLEGEGGLFE+IE 
Sbjct: 247 AHVEAESNENGIGFVKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIER 306

Query: 353 CLEKQGHMVIVIAE---------------------------------------------- 412
            L+  GHMVIV+AE                                              
Sbjct: 307 RLKDHGHMVIVLAEGAGQDLMCKSMESTPMDASGNKLLKDVGLWLSQSIKDHFKKNKMVM 366

Query: 413 -----DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRII 451
                DPTYMIRAVPS +SDN++CTLLA SA+HGAMAGYTG+T G VNG+  YIPF+RI 
Sbjct: 367 NLKYIDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRIT 426

BLAST of MS026369 vs. ExPASy Swiss-Prot
Match: Q9M076 (ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE=1 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.1e-168
Identity = 297/444 (66.89%), Postives = 344/444 (77.48%), Query Frame = 0

Query: 56  EIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIVVEK 115
           ++  G   YVLEDVPHL DY+ +L  YPNP L +N+AYS   QYFV+ DD V +KIVV K
Sbjct: 11  KLVEGPAGYVLEDVPHLSDYILDLPTYPNP-LQSNAAYSVVRQYFVDEDDTVQEKIVVHK 70

Query: 116 NSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHKIVG 175
           +S  G +FR AGPRQKV+F+P DVRACIVTCGGLCPGLNTVIREIVCGL+ MYGV +++G
Sbjct: 71  DSPRGTHFRRAGPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMYGVTEVIG 130

Query: 176 IEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVYILG 235
           ++ G++GFY +NT++LTPK+V++IHKRGGTILGTSRGGH+TS IVD+IQDR INQVYI+G
Sbjct: 131 VDCGFRGFYSKNTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDREINQVYIIG 190

Query: 236 GDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDAAHV 295
           GDGTQKGA AIY+EIRRRGLKVAV GIPKTIDNDIPVIDKSFGFDTAVEEAQRAI+AAHV
Sbjct: 191 GDGTQKGANAIYKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHV 250

Query: 296 EAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLE 355
           EA S ENGIG+VKLMGR SGFIAMYA LASRDVDCCLIPESPFYLEG+GGL+E+I   L 
Sbjct: 251 EATSVENGIGIVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGLYEFIAKRLR 310

Query: 356 KQGHMVIVIAE------------------------------------------------- 415
           + GHMVIVIAE                                                 
Sbjct: 311 ENGHMVIVIAEGAGQDLVAESIEQQDASGNKLLKDVGLWMSLKIKEYFAKHNVMDITLKY 370

Query: 416 -DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRIIERQN 450
            DPTYMIRA+P+ +SDN++ TLLA SA+HGAMAGYTGF  G VNG+H YIPF+RI ERQN
Sbjct: 371 IDPTYMIRAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHTYIPFNRITERQN 430

BLAST of MS026369 vs. ExPASy Swiss-Prot
Match: Q9M0F9 (ATP-dependent 6-phosphofructokinase 1 OS=Arabidopsis thaliana OX=3702 GN=PFK1 PE=1 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 8.9e-166
Identity = 296/448 (66.07%), Postives = 341/448 (76.12%), Query Frame = 0

Query: 52  HSKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKI 111
           +S ++I +G   Y+LEDVPH  D  P+   YPNP L +N+AYS  +QYFV+ DD V QKI
Sbjct: 7   NSDRKIVTGPAGYILEDVPHFSDDFPDHPTYPNP-LQDNAAYSVVKQYFVDEDDTVPQKI 66

Query: 112 VVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVH 171
           VV  +S  G +FR AGPRQ+V+FE +DV ACIVTCGGLCPGLNTVIREIVCGL++MYGV 
Sbjct: 67  VVHPDSPRGTHFRRAGPRQRVYFESDDVLACIVTCGGLCPGLNTVIREIVCGLSYMYGVK 126

Query: 172 KIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQV 231
           +I+GI+GGY+GFY  NTI L  K+VN+IH+ GGTILGTSRGGHNT+ IVDSIQDRGINQV
Sbjct: 127 RILGIDGGYRGFYARNTIHLDLKTVNDIHRSGGTILGTSRGGHNTTKIVDSIQDRGINQV 186

Query: 232 YILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAID 291
           YI+GGDG+QKGAAAI+EEIR+R LKVAV GIPKTIDNDIP+ID+SFGFDTAVEEAQRAI+
Sbjct: 187 YIIGGDGSQKGAAAIFEEIRKRKLKVAVAGIPKTIDNDIPIIDRSFGFDTAVEEAQRAIN 246

Query: 292 AAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIE 351
           AAHVEA S ENGIGLVKLMGR SGFIAM+A LASRDVDCCLIPESPF+LEG GGLFE+I+
Sbjct: 247 AAHVEATSFENGIGLVKLMGRYSGFIAMHATLASRDVDCCLIPESPFFLEGSGGLFEFID 306

Query: 352 HCLEKQGHMVIVIAE--------------------------------------------- 411
             L++ GHMVIVIAE                                             
Sbjct: 307 KRLKESGHMVIVIAEGAGQDLLSESMKESTTLKDASGNKLLQDIGLWISQRIKDHFAKKM 366

Query: 412 -------DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHR 448
                  DPTYMIRAVPS +SDN+ CTLLA SA+HG MAGY GFTVG VNG+H YIPF+R
Sbjct: 367 TLTLKYIDPTYMIRAVPSNASDNVCCTLLAQSAVHGVMAGYNGFTVGLVNGRHTYIPFNR 426

BLAST of MS026369 vs. ExPASy Swiss-Prot
Match: Q9FKG3 (ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PFK4 PE=1 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 6.2e-159
Identity = 280/440 (63.64%), Postives = 333/440 (75.68%), Query Frame = 0

Query: 61  DFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIVVEKNSLSG 120
           D  +VLEDVPHL  +LP+L +YPNP L  + AY+  ++ FV+ +DVVAQ IVV+K S  G
Sbjct: 66  DDGFVLEDVPHLTKFLPDLPSYPNP-LKESQAYAIVKRTFVSSEDVVAQNIVVQKGSKRG 125

Query: 121 AYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHKIVGIEGGY 180
            +FR AGPR++V+F  ++V+ACIVTCGGLCPG+NTVIREIVCGLN+MYGV+ I+GI+GGY
Sbjct: 126 VHFRRAGPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGGY 185

Query: 181 KGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVYILGGDGTQ 240
           +GFY +NT++LTPK VN+IHKRGGT L TSRGGH+T+ IVD+IQDRGINQVYI+GG GTQ
Sbjct: 186 RGFYSKNTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQ 245

Query: 241 KGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDAAHVEAESN 300
           KGA  IYEE+ RRGL+VAV GIPKTIDNDI VIDKSFGFDTAVEEAQRAI+AAHVE ES 
Sbjct: 246 KGAEKIYEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESV 305

Query: 301 ENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLEKQGHM 360
           ENG+G+VKLMGR SGFIAM A LA+RDVDCCLIPESPF+LEG+GGLFE+IE  L++  HM
Sbjct: 306 ENGVGIVKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRHM 365

Query: 361 VIVIAE-----------------------------------------------------D 420
           VIVIAE                                                     D
Sbjct: 366 VIVIAEGAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRKMMINMKYID 425

Query: 421 PTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRIIERQNKV 448
           PTYMIRA+PS +SDN++CTLLA SA+HGAMAGY+GFTVGPVN +H YIP  ++ E  N V
Sbjct: 426 PTYMIRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTNTV 485

BLAST of MS026369 vs. ExPASy TrEMBL
Match: A0A6J1DJC0 (ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC111021058 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.1e-232
Identity = 410/464 (88.36%), Postives = 410/464 (88.36%), Query Frame = 0

Query: 47  MDDHDHSKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDV 106
           MDDHDHSKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDV
Sbjct: 1   MDDHDHSKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDV 60

Query: 107 VAQKIVVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNH 166
           VAQKIVVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIV GLNH
Sbjct: 61  VAQKIVVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVGGLNH 120

Query: 167 MYGVHKIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDR 226
           MYGVHKIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDR
Sbjct: 121 MYGVHKIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDR 180

Query: 227 GINQVYILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEA 286
           GINQVYILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEA
Sbjct: 181 GINQVYILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEA 240

Query: 287 QRAIDAAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGL 346
           QRAIDAAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGL
Sbjct: 241 QRAIDAAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGL 300

Query: 347 FEYIEHCLEKQGHMVIVIAE---------------------------------------- 406
           FEYIEHCLEKQGHMVIVIAE                                        
Sbjct: 301 FEYIEHCLEKQGHMVIVIAEGAGQELVSKSTQKADQLDASGNKLHQDVGSWISQQIKNHF 360

Query: 407 -------------DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHV 458
                        DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHV
Sbjct: 361 EEKGTIPINLKYIDPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHV 420

BLAST of MS026369 vs. ExPASy TrEMBL
Match: A0A6J1DJI3 (ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC111021059 PE=3 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 1.1e-195
Identity = 361/506 (71.34%), Postives = 390/506 (77.08%), Query Frame = 0

Query: 1   MDASSTSISSSFLSLFSPRFSGICWNWFDKSTPSNHRRCGSSSSSTMDDHDHSKKEIFSG 60
           MD+S +S SSS  S F   FS           P +HRR  +  SS     DH+ ++   G
Sbjct: 1   MDSSVSSFSSSSTS-FDFNFSA--------PGPDHHRRFQNGESSPSSAMDHNGRDA-PG 60

Query: 61  DFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIVVEKNSLSG 120
           DFRYVLEDVPHL DYLP+L AYPNP L +N AYS  +QYFV++DD VAQ +VV KN+  G
Sbjct: 61  DFRYVLEDVPHLTDYLPDLPAYPNP-LQDNPAYSVVKQYFVDVDDAVAQSVVVHKNTPRG 120

Query: 121 AYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHKIVGIEGGY 180
            +FR  GPRQKVFFE ++V ACIVTCGGLCPGLNTVIREIVCGL HMYGVHK+VGIEGGY
Sbjct: 121 THFRRTGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVHKVVGIEGGY 180

Query: 181 KGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVYILGGDGTQ 240
           KGFY  NTISLTPKSVNNIHKRGGTILGTSRGGHNT+ IVDSIQ RGINQVYILGGDGTQ
Sbjct: 181 KGFYARNTISLTPKSVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQVYILGGDGTQ 240

Query: 241 KGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDAAHVEAESN 300
           KGAAAI+EEIRRRGLKVAV GIPKTIDNDIPVIDKSFGFDTAVEEAQRAI+AAHVE+ESN
Sbjct: 241 KGAAAIFEEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVESESN 300

Query: 301 ENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLEKQGHM 360
           EN IGLVKLMGRNSGFIAMYA LASRDVDCCLIPESPFYLEGEGGLFEYIEHCL++QGHM
Sbjct: 301 ENCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLKEQGHM 360

Query: 361 VIVIAE-----------------------------------------------------D 420
           VIVIAE                                                     D
Sbjct: 361 VIVIAEGAGQELISKSMHNMDNQDASGNKLHQDVGLWISQRIKDYFKEMRKIAIDLKYID 420

Query: 421 PTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRIIERQNKV 454
           PTYMIRAVPSI+SDNIFC+LLA SAIHGAMAGYTGFTVGPVNG+H YIPFHRIIERQNKV
Sbjct: 421 PTYMIRAVPSIASDNIFCSLLAQSAIHGAMAGYTGFTVGPVNGRHAYIPFHRIIERQNKV 480

BLAST of MS026369 vs. ExPASy TrEMBL
Match: A0A6J1DHV8 (ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC111021059 PE=3 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.3e-191
Identity = 361/531 (67.98%), Postives = 391/531 (73.63%), Query Frame = 0

Query: 1   MDASSTSISSSFLSLFSPRFSGICWNWFDKSTPSNHRRCGSSSSSTMDDHDHSKKEIFSG 60
           MD+S +S SSS  S F   FS           P +HRR  +  SS     DH+ ++   G
Sbjct: 1   MDSSVSSFSSSSTS-FDFNFSA--------PGPDHHRRFQNGESSPSSAMDHNGRDA-PG 60

Query: 61  DFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQ----------- 120
           DFRYVLEDVPHL DYLP+L AYPNP L +N AYS  +QYFV++DD VAQ           
Sbjct: 61  DFRYVLEDVPHLTDYLPDLPAYPNP-LQDNPAYSVVKQYFVDVDDAVAQSVSNLCLNSKV 120

Query: 121 --------------KIVVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNT 180
                         ++VV KN+  G +FR  GPRQKVFFE ++V ACIVTCGGLCPGLNT
Sbjct: 121 LDGIVLNFVGFCSKQVVVHKNTPRGTHFRRTGPRQKVFFESDEVHACIVTCGGLCPGLNT 180

Query: 181 VIREIVCGLNHMYGVHKIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHN 240
           VIREIVCGL HMYGVHK+VGIEGGYKGFY  NTISLTPKSVNNIHKRGGTILGTSRGGHN
Sbjct: 181 VIREIVCGLYHMYGVHKVVGIEGGYKGFYARNTISLTPKSVNNIHKRGGTILGTSRGGHN 240

Query: 241 TSNIVDSIQDRGINQVYILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDK 300
           T+ IVDSIQ RGINQVYILGGDGTQKGAAAI+EEIRRRGLKVAV GIPKTIDNDIPVIDK
Sbjct: 241 TTKIVDSIQHRGINQVYILGGDGTQKGAAAIFEEIRRRGLKVAVAGIPKTIDNDIPVIDK 300

Query: 301 SFGFDTAVEEAQRAIDAAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPE 360
           SFGFDTAVEEAQRAI+AAHVE+ESNEN IGLVKLMGRNSGFIAMYA LASRDVDCCLIPE
Sbjct: 301 SFGFDTAVEEAQRAINAAHVESESNENCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPE 360

Query: 361 SPFYLEGEGGLFEYIEHCLEKQGHMVIVIAE----------------------------- 420
           SPFYLEGEGGLFEYIEHCL++QGHMVIVIAE                             
Sbjct: 361 SPFYLEGEGGLFEYIEHCLKEQGHMVIVIAEGAGQELISKSMHNMDNQDASGNKLHQDVG 420

Query: 421 ------------------------DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTG 454
                                   DPTYMIRAVPSI+SDNIFC+LLA SAIHGAMAGYTG
Sbjct: 421 LWISQRIKDYFKEMRKIAIDLKYIDPTYMIRAVPSIASDNIFCSLLAQSAIHGAMAGYTG 480

BLAST of MS026369 vs. ExPASy TrEMBL
Match: A0A5A7U6H3 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PFK PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 4.1e-190
Identity = 338/447 (75.62%), Postives = 360/447 (80.54%), Query Frame = 0

Query: 60  GDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIVVEKNSLS 119
           GD+ YVLEDVPHL DYLP+L A  NP L ++ AYSA +QYFVN+DD VAQ +VV  NS  
Sbjct: 19  GDYHYVLEDVPHLTDYLPDLPANLNP-LQDHPAYSAVKQYFVNVDDAVAQNVVVHNNSPR 78

Query: 120 GAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHKIVGIEGG 179
           G +FR AGPRQKVFFE ++V ACIVTCGGLCPGLNTVIREIVCGL HMYGVHK+VGIEGG
Sbjct: 79  GTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVHKVVGIEGG 138

Query: 180 YKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVYILGGDGT 239
           Y+GFY  NTI LTPKSVNNIHKRGGTILGTSRGGHNT+ IVDSIQ RGINQVYILGGDGT
Sbjct: 139 YRGFYARNTIPLTPKSVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQVYILGGDGT 198

Query: 240 QKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDAAHVEAES 299
           QKGAAAI+EEIRRRGLKVAV GIPKTIDNDIPVIDKSFGFDTAVEEAQRAI AAHVE+ES
Sbjct: 199 QKGAAAIFEEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESES 258

Query: 300 NENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLEKQGH 359
           NENGIGLVKLMGRNSGFIAMYA LASRDVDCCLIPESPFYLEGEGGLFEYIEHC+++ GH
Sbjct: 259 NENGIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIKEHGH 318

Query: 360 MVIVIAE----------------------------------------------------- 419
           MVIVIAE                                                     
Sbjct: 319 MVIVIAEGAGQELMSESIHKLDQQDASGNKLHQDVGLWISQQIKDFFKEKRKMVINLKYI 378

Query: 420 DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRIIERQNK 454
           DPTYMIRAVPSI+SDNIFCTLLA SAIHGAMAGYTGFTVGPVNG+H YIPFHRIIERQNK
Sbjct: 379 DPTYMIRAVPSIASDNIFCTLLAQSAIHGAMAGYTGFTVGPVNGRHAYIPFHRIIERQNK 438

BLAST of MS026369 vs. ExPASy TrEMBL
Match: A0A5D3CB91 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PFK PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 4.1e-190
Identity = 338/447 (75.62%), Postives = 360/447 (80.54%), Query Frame = 0

Query: 60  GDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIVVEKNSLS 119
           GD+ YVLEDVPHL DYLP+L A  NP L ++ AYSA +QYFVN+DD VAQ +VV  NS  
Sbjct: 19  GDYHYVLEDVPHLTDYLPDLPANLNP-LQDHPAYSAVKQYFVNVDDAVAQNVVVHNNSPR 78

Query: 120 GAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHKIVGIEGG 179
           G +FR AGPRQKVFFE ++V ACIVTCGGLCPGLNTVIREIVCGL HMYGVHK+VGIEGG
Sbjct: 79  GTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVHKVVGIEGG 138

Query: 180 YKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVYILGGDGT 239
           Y+GFY  NTI LTPKSVNNIHKRGGTILGTSRGGHNT+ IVDSIQ RGINQVYILGGDGT
Sbjct: 139 YRGFYARNTIPLTPKSVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQVYILGGDGT 198

Query: 240 QKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDAAHVEAES 299
           QKGAAAI+EEIRRRGLKVAV GIPKTIDNDIPVIDKSFGFDTAVEEAQRAI AAHVE+ES
Sbjct: 199 QKGAAAIFEEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESES 258

Query: 300 NENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLEKQGH 359
           NENGIGLVKLMGRNSGFIAMYA LASRDVDCCLIPESPFYLEGEGGLFEYIEHC+++ GH
Sbjct: 259 NENGIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIKEHGH 318

Query: 360 MVIVIAE----------------------------------------------------- 419
           MVIVIAE                                                     
Sbjct: 319 MVIVIAEGAGQELMSESIHKLDQQDASGNKLHQDVGLWISQQIKDFFKEKRKMVINLKYI 378

Query: 420 DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRIIERQNK 454
           DPTYMIRAVPSI+SDNIFCTLLA SAIHGAMAGYTGFTVGPVNG+H YIPFHRIIERQNK
Sbjct: 379 DPTYMIRAVPSIASDNIFCTLLAQSAIHGAMAGYTGFTVGPVNGRHAYIPFHRIIERQNK 438

BLAST of MS026369 vs. TAIR 10
Match: AT4G26270.1 (phosphofructokinase 3 )

HSP 1 Score: 606.3 bits (1562), Expect = 2.0e-173
Identity = 308/458 (67.25%), Postives = 349/458 (76.20%), Query Frame = 0

Query: 47  MDDHDHSKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDV 106
           M   + SK +I +G   YVLEDVPHL DYLP L  YPNP L +N AYS  +QYFV+ DD 
Sbjct: 1   MSTVESSKPKIINGSCGYVLEDVPHLSDYLPGLPTYPNP-LQDNPAYSVVKQYFVDADDS 60

Query: 107 VAQKIVVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNH 166
           V QKIVV K+   G +FR AGPRQKV+FE ++V ACIVTCGGLCPGLNTVIREIV  L++
Sbjct: 61  VPQKIVVHKDGPRGIHFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSY 120

Query: 167 MYGVHKIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDR 226
           MYGV +I+GI+GGY+GFY +NT+SL  K VN+IHKRGGTILGTSRGGH+T+ IVDSIQDR
Sbjct: 121 MYGVKRILGIDGGYRGFYAKNTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDR 180

Query: 227 GINQVYILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEA 286
           GINQVYI+GGDGTQ+GA+ I+EEIRRRGLKVAV+GIPKTIDNDIPVIDKSFGFDTAVEEA
Sbjct: 181 GINQVYIIGGDGTQRGASVIFEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEA 240

Query: 287 QRAIDAAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGL 346
           QRAI+AAHVEAES ENGIG+VKLMGR SGFIAMYA LASRDVDCCLIPESPFYLEGEGGL
Sbjct: 241 QRAINAAHVEAESIENGIGVVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGL 300

Query: 347 FEYIEHCLEKQGHMVIVIAE---------------------------------------- 406
           FEYIE  L++ GHMV+VIAE                                        
Sbjct: 301 FEYIEKRLKESGHMVLVIAEGAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHF 360

Query: 407 ------------DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVY 453
                       DPTYMIRAVPS +SDN++CTLLA SA+HGAMAGYTG+  G VNG+  Y
Sbjct: 361 NQKKMVMNLKYIDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTY 420

BLAST of MS026369 vs. TAIR 10
Match: AT5G56630.1 (phosphofructokinase 7 )

HSP 1 Score: 600.1 bits (1546), Expect = 1.5e-171
Identity = 301/449 (67.04%), Postives = 345/449 (76.84%), Query Frame = 0

Query: 53  SKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIV 112
           +K +I +G   Y+L+DVPHLIDYLP+L  YPNP L +N AYS  +QYFV+ DD V +K+V
Sbjct: 7   NKPKIVNGPGGYILQDVPHLIDYLPDLPTYPNP-LQDNPAYSVVKQYFVHADDSVPEKVV 66

Query: 113 VEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHK 172
           V K+   G +FR AGPRQKV+FE ++V ACIVTCGGLCPGLNTVIRE+V  L++MYGV +
Sbjct: 67  VHKDGPRGVHFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKR 126

Query: 173 IVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVY 232
           I+GI+GGY+GFY +NTI L  K VN+IHKRGGTI+GTSRGGH+T+ IVDSIQDRGINQVY
Sbjct: 127 ILGIDGGYRGFYAKNTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVY 186

Query: 233 ILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDA 292
           I+GGDGTQ+GA+ I+EEIRRR LKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAI+A
Sbjct: 187 IIGGDGTQRGASVIFEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINA 246

Query: 293 AHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEH 352
           AHVEAESNENGIG VKLMGR SG+IAMYA LASRDVDCCLIPESPFYLEGEGGLFE+IE 
Sbjct: 247 AHVEAESNENGIGFVKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIER 306

Query: 353 CLEKQGHMVIVIAE---------------------------------------------- 412
            L+  GHMVIV+AE                                              
Sbjct: 307 RLKDHGHMVIVLAEGAGQDLMCKSMESTPMDASGNKLLKDVGLWLSQSIKDHFKKNKMVM 366

Query: 413 -----DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRII 451
                DPTYMIRAVPS +SDN++CTLLA SA+HGAMAGYTG+T G VNG+  YIPF+RI 
Sbjct: 367 NLKYIDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRIT 426

BLAST of MS026369 vs. TAIR 10
Match: AT4G32840.1 (phosphofructokinase 6 )

HSP 1 Score: 594.3 bits (1531), Expect = 8.0e-170
Identity = 297/444 (66.89%), Postives = 344/444 (77.48%), Query Frame = 0

Query: 56  EIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIVVEK 115
           ++  G   YVLEDVPHL DY+ +L  YPNP L +N+AYS   QYFV+ DD V +KIVV K
Sbjct: 11  KLVEGPAGYVLEDVPHLSDYILDLPTYPNP-LQSNAAYSVVRQYFVDEDDTVQEKIVVHK 70

Query: 116 NSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHKIVG 175
           +S  G +FR AGPRQKV+F+P DVRACIVTCGGLCPGLNTVIREIVCGL+ MYGV +++G
Sbjct: 71  DSPRGTHFRRAGPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMYGVTEVIG 130

Query: 176 IEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVYILG 235
           ++ G++GFY +NT++LTPK+V++IHKRGGTILGTSRGGH+TS IVD+IQDR INQVYI+G
Sbjct: 131 VDCGFRGFYSKNTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDREINQVYIIG 190

Query: 236 GDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDAAHV 295
           GDGTQKGA AIY+EIRRRGLKVAV GIPKTIDNDIPVIDKSFGFDTAVEEAQRAI+AAHV
Sbjct: 191 GDGTQKGANAIYKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHV 250

Query: 296 EAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLE 355
           EA S ENGIG+VKLMGR SGFIAMYA LASRDVDCCLIPESPFYLEG+GGL+E+I   L 
Sbjct: 251 EATSVENGIGIVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGLYEFIAKRLR 310

Query: 356 KQGHMVIVIAE------------------------------------------------- 415
           + GHMVIVIAE                                                 
Sbjct: 311 ENGHMVIVIAEGAGQDLVAESIEQQDASGNKLLKDVGLWMSLKIKEYFAKHNVMDITLKY 370

Query: 416 -DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRIIERQN 450
            DPTYMIRA+P+ +SDN++ TLLA SA+HGAMAGYTGF  G VNG+H YIPF+RI ERQN
Sbjct: 371 IDPTYMIRAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHTYIPFNRITERQN 430

BLAST of MS026369 vs. TAIR 10
Match: AT4G29220.1 (phosphofructokinase 1 )

HSP 1 Score: 584.7 bits (1506), Expect = 6.4e-167
Identity = 296/448 (66.07%), Postives = 341/448 (76.12%), Query Frame = 0

Query: 52  HSKKEIFSGDFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKI 111
           +S ++I +G   Y+LEDVPH  D  P+   YPNP L +N+AYS  +QYFV+ DD V QKI
Sbjct: 7   NSDRKIVTGPAGYILEDVPHFSDDFPDHPTYPNP-LQDNAAYSVVKQYFVDEDDTVPQKI 66

Query: 112 VVEKNSLSGAYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVH 171
           VV  +S  G +FR AGPRQ+V+FE +DV ACIVTCGGLCPGLNTVIREIVCGL++MYGV 
Sbjct: 67  VVHPDSPRGTHFRRAGPRQRVYFESDDVLACIVTCGGLCPGLNTVIREIVCGLSYMYGVK 126

Query: 172 KIVGIEGGYKGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQV 231
           +I+GI+GGY+GFY  NTI L  K+VN+IH+ GGTILGTSRGGHNT+ IVDSIQDRGINQV
Sbjct: 127 RILGIDGGYRGFYARNTIHLDLKTVNDIHRSGGTILGTSRGGHNTTKIVDSIQDRGINQV 186

Query: 232 YILGGDGTQKGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAID 291
           YI+GGDG+QKGAAAI+EEIR+R LKVAV GIPKTIDNDIP+ID+SFGFDTAVEEAQRAI+
Sbjct: 187 YIIGGDGSQKGAAAIFEEIRKRKLKVAVAGIPKTIDNDIPIIDRSFGFDTAVEEAQRAIN 246

Query: 292 AAHVEAESNENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIE 351
           AAHVEA S ENGIGLVKLMGR SGFIAM+A LASRDVDCCLIPESPF+LEG GGLFE+I+
Sbjct: 247 AAHVEATSFENGIGLVKLMGRYSGFIAMHATLASRDVDCCLIPESPFFLEGSGGLFEFID 306

Query: 352 HCLEKQGHMVIVIAE--------------------------------------------- 411
             L++ GHMVIVIAE                                             
Sbjct: 307 KRLKESGHMVIVIAEGAGQDLLSESMKESTTLKDASGNKLLQDIGLWISQRIKDHFAKKM 366

Query: 412 -------DPTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHR 448
                  DPTYMIRAVPS +SDN+ CTLLA SA+HG MAGY GFTVG VNG+H YIPF+R
Sbjct: 367 TLTLKYIDPTYMIRAVPSNASDNVCCTLLAQSAVHGVMAGYNGFTVGLVNGRHTYIPFNR 426

BLAST of MS026369 vs. TAIR 10
Match: AT5G61580.1 (phosphofructokinase 4 )

HSP 1 Score: 562.0 bits (1447), Expect = 4.4e-160
Identity = 280/440 (63.64%), Postives = 333/440 (75.68%), Query Frame = 0

Query: 61  DFRYVLEDVPHLIDYLPNLSAYPNPLLNNNSAYSATEQYFVNMDDVVAQKIVVEKNSLSG 120
           D  +VLEDVPHL  +LP+L +YPNP L  + AY+  ++ FV+ +DVVAQ IVV+K S  G
Sbjct: 66  DDGFVLEDVPHLTKFLPDLPSYPNP-LKESQAYAIVKRTFVSSEDVVAQNIVVQKGSKRG 125

Query: 121 AYFRLAGPRQKVFFEPEDVRACIVTCGGLCPGLNTVIREIVCGLNHMYGVHKIVGIEGGY 180
            +FR AGPR++V+F  ++V+ACIVTCGGLCPG+NTVIREIVCGLN+MYGV+ I+GI+GGY
Sbjct: 126 VHFRRAGPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGGY 185

Query: 181 KGFYGENTISLTPKSVNNIHKRGGTILGTSRGGHNTSNIVDSIQDRGINQVYILGGDGTQ 240
           +GFY +NT++LTPK VN+IHKRGGT L TSRGGH+T+ IVD+IQDRGINQVYI+GG GTQ
Sbjct: 186 RGFYSKNTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQ 245

Query: 241 KGAAAIYEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIDAAHVEAESN 300
           KGA  IYEE+ RRGL+VAV GIPKTIDNDI VIDKSFGFDTAVEEAQRAI+AAHVE ES 
Sbjct: 246 KGAEKIYEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESV 305

Query: 301 ENGIGLVKLMGRNSGFIAMYACLASRDVDCCLIPESPFYLEGEGGLFEYIEHCLEKQGHM 360
           ENG+G+VKLMGR SGFIAM A LA+RDVDCCLIPESPF+LEG+GGLFE+IE  L++  HM
Sbjct: 306 ENGVGIVKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRHM 365

Query: 361 VIVIAE-----------------------------------------------------D 420
           VIVIAE                                                     D
Sbjct: 366 VIVIAEGAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRKMMINMKYID 425

Query: 421 PTYMIRAVPSISSDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGQHVYIPFHRIIERQNKV 448
           PTYMIRA+PS +SDN++CTLLA SA+HGAMAGY+GFTVGPVN +H YIP  ++ E  N V
Sbjct: 426 PTYMIRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTNTV 485

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153587.12.4e-23288.36ATP-dependent 6-phosphofructokinase 3-like [Momordica charantia][more]
XP_022153589.12.3e-19571.34ATP-dependent 6-phosphofructokinase 6-like isoform X2 [Momordica charantia][more]
XP_022153588.12.7e-19167.98ATP-dependent 6-phosphofructokinase 6-like isoform X1 [Momordica charantia][more]
KAA0050858.18.5e-19075.62ATP-dependent 6-phosphofructokinase 6-like [Cucumis melo var. makuwa][more]
TYK08494.18.5e-19075.62ATP-dependent 6-phosphofructokinase 6-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q94AA42.9e-17267.25ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE... [more]
Q9C5J72.1e-17067.04ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE... [more]
Q9M0761.1e-16866.89ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE... [more]
Q9M0F98.9e-16666.07ATP-dependent 6-phosphofructokinase 1 OS=Arabidopsis thaliana OX=3702 GN=PFK1 PE... [more]
Q9FKG36.2e-15963.64ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1DJC01.1e-23288.36ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC1110210... [more]
A0A6J1DJI31.1e-19571.34ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC1110210... [more]
A0A6J1DHV81.3e-19167.98ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC1110210... [more]
A0A5A7U6H34.1e-19075.62ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PF... [more]
A0A5D3CB914.1e-19075.62ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PF... [more]
Match NameE-valueIdentityDescription
AT4G26270.12.0e-17367.25phosphofructokinase 3 [more]
AT5G56630.11.5e-17167.04phosphofructokinase 7 [more]
AT4G32840.18.0e-17066.89phosphofructokinase 6 [more]
AT4G29220.16.4e-16766.07phosphofructokinase 1 [more]
AT5G61580.14.4e-16063.64phosphofructokinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022953ATP-dependent 6-phosphofructokinasePRINTSPR00476PHFRCTKINASEcoord: 278..296
score: 34.14
coord: 260..277
score: 45.87
coord: 143..162
score: 42.31
coord: 227..243
score: 43.89
coord: 356..368
score: 25.64
coord: 299..315
score: 29.41
IPR012004Pyrophosphate-dependent phosphofructokinase TP0108-typePIRSFPIRSF000534ATP_PFK_TP0108coord: 58..366
e-value: 1.8E-146
score: 486.1
coord: 367..451
e-value: 7.5E-32
score: 108.3
IPR000023Phosphofructokinase domainPFAMPF00365PFKcoord: 142..368
e-value: 2.9E-59
score: 200.7
NoneNo IPR availableGENE3D3.40.50.450coord: 135..325
e-value: 1.1E-61
score: 209.4
NoneNo IPR availablePANTHERPTHR45770:SF15ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASEcoord: 367..456
coord: 20..366
NoneNo IPR availablePANTHERPTHR45770FAMILY NOT NAMEDcoord: 367..456
coord: 20..366
IPR035966Phosphofructokinase superfamilySUPERFAMILY53784Phosphofructokinasecoord: 75..447

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS026369.1MS026369.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006002 fructose 6-phosphate metabolic process
biological_process GO:0006096 glycolytic process
molecular_function GO:0003872 6-phosphofructokinase activity
molecular_function GO:0005524 ATP binding