MS026285 (gene) Bitter gourd (TR) v1

BLAST of MS026285 vs. NCBI nr
Match: XP_022159381.1 (subtilisin-like protease SBT3.4, partial [Momordica charantia])

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 588/603 (97.51%), Postives = 590/603 (97.84%), Query Frame = 0

Query: 99  MAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGEDVIIGVLDSGIWPESE 158
           MAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGE VIIGVLDSGIWPESE
Sbjct: 1   MAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGEHVIIGVLDSGIWPESE 60

Query: 159 AFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGLIADGGQQALANEYLSAR 218
           AFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKG IAD GQ ALANEY SAR
Sbjct: 61  AFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGXIADXGQXALANEYXSAR 120

Query: 219 DFQGHGTHVASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLWSEGAGSGADILMA 278
           DF GHGTH ASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLWSEGAGSGADILMA
Sbjct: 121 DFXGHGTHXASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLWSEGAGSGADILMA 180

Query: 279 MDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQT 338
           MDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQT
Sbjct: 181 MDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQT 240

Query: 339 IVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCK 398
           IVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCK
Sbjct: 241 IVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCK 300

Query: 399 DILGNDTSLSGNIVLCFNKLVDVETTQKAVVAVRQANGNGIIVAGQHDDILFPYGADFPC 458
           DILGNDTSLSGNIVLCFNKLVD+E TQKAVVAVRQANGNGIIVAGQHDDILFP GADFPC
Sbjct: 301 DILGNDTSLSGNIVLCFNKLVDLEMTQKAVVAVRQANGNGIIVAGQHDDILFPCGADFPC 360

Query: 459 IVLDPDVGTKFFHYLLGTSNPMVRIGRATTIVGKPISTNVAFFSSRGPNSASPAILKQPD 518
           IVLDPDVGTKFFHY L TSNPMVRIGRA TIVGKPISTNVAFFSSRGPNSASPAILK PD
Sbjct: 361 IVLDPDVGTKFFHYXLDTSNPMVRIGRAXTIVGKPISTNVAFFSSRGPNSASPAILK-PD 420

Query: 519 IAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALS 578
           IAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSA+S
Sbjct: 421 IAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSAJS 480

Query: 579 TTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGM 638
           TTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGM
Sbjct: 481 TTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGM 540

Query: 639 GYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAV 698
           GYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAV
Sbjct: 541 GYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAV 600

Query: 699 IRA 702
           IRA
Sbjct: 601 IRA 602

BLAST of MS026285 vs. NCBI nr
Match: XP_022984814.1 (subtilisin-like protease SBT3.9 [Cucurbita maxima])

HSP 1 Score: 966.8 bits (2498), Expect = 1.1e-277
Identity = 498/760 (65.53%), Postives = 595/760 (78.29%), Query Frame = 0

Query: 6   FIAAFVSFYALFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVLGS 65
           FI A V+ YA+FS      MAEADDQN KVHIVYLGE  ++D KLTT++HHELL +VLGS
Sbjct: 9   FILAVVTIYAVFS-----DMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLESVLGS 68

Query: 66  KEKSWESMVYSYRHGFSGFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLS 125
           KEKS ESMVYSYRHGFSGFAAKLTNSQAQKLA M  V RV P+SLYKM+TTRSWDFLGLS
Sbjct: 69  KEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWDFLGLS 128

Query: 126 TSPSQSSNLLHRGKMGEDVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFNST 185
           +SPS+SSNL HR KMG++VIIGV+D+G WPESE+F D+GMGP+PSRWKG C+SGE+FNS+
Sbjct: 129 SSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDFNSS 188

Query: 186 NCNRKIIGARWYNKGLIADGGQQALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGLG 245
           +CN+K+IGARW+   LIAD G++A+  +YLSARD +GHGTH ASTA G+FV NV+Y G G
Sbjct: 189 HCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVRNVSYFGNG 248

Query: 246 AGTVRGGAPRACLAIYKVLWSEG-AGSGADILMAMDDAIHDGVDVLSLSLRFGGVPISPE 305
            GT+RGGAP A LAIYKVLWS+   GSGADIL  +D+AIHDGVDVLS+S+    +P+ P+
Sbjct: 249 RGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIG-KSIPLFPD 308

Query: 306 FSEDSVVAIGAFHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPV-T 365
            ++ + VA+G+FHA A+GISVVCAGGN+G  +QT+ N APW+FTVAASTIDRAFL  + T
Sbjct: 309 VNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLVSITT 368

Query: 366 LGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCKDILGNDTSLSGNIV-LCFNKLVDVE 425
           LGDN T+LGQ F     DIVG LV    R    C  ILG D  +SGN+V LCF  L    
Sbjct: 369 LGDNATYLGQMFL--RKDIVGMLVAMDGR----CAGILGGDIPISGNVVLLCFTDLAKTA 428

Query: 426 TTQKAVVAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPMVR 485
               AV+  +QA   G+I AGQ  D L P   D PCI +D  VGTK F YLL   + ++R
Sbjct: 429 PASNAVMPGKQAKVVGVIYAGQQTDNLVP--CDIPCIHVDTHVGTKLFTYLLNDDDALIR 488

Query: 486 IGRATTIVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKGFA 545
           +    TI+GKPIS+ +A+FSSRGPNS SP ILK PDIAAPG+NI+AA+ P +   DKGFA
Sbjct: 489 LRATRTIIGKPISSRIAYFSSRGPNSFSPGILK-PDIAAPGANIIAAVPPNHKGGDKGFA 548

Query: 546 FQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKLAD 605
             SGTSMA PHISGIV L+KSLHPTWSPAAI+SAL TTARV+ PSG PI AE SPPK+AD
Sbjct: 549 TMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSPPKVAD 608

Query: 606 PFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSV 665
           PFDYGGG+V+ NAA DPGLIYDL   DY+ YYLC MGY  +DIS L++QKT CPS+R+S+
Sbjct: 609 PFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASI 668

Query: 666 LDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTVKK 725
           LDLNLP+ITVPAL NSTTVTRTVTNVGN ++VY+AVI+AP G+KV V+P +L FN  VKK
Sbjct: 669 LDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK 728

Query: 726 ISFKVTISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTD 763
           ISFKV  S+++ RNYGY FGSL W+DG+H VK PLSVR D
Sbjct: 729 ISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 753

BLAST of MS026285 vs. NCBI nr
Match: XP_023552781.1 (subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 959.5 bits (2479), Expect = 1.7e-275
Identity = 498/759 (65.61%), Postives = 596/759 (78.52%), Query Frame = 0

Query: 7   IAAFVSFYALFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVLGSK 66
           + A V+ Y +FS     +MAEADDQN KVHIVYLGE  ++D KLTT++HHELL +VLGSK
Sbjct: 12  VVAVVTIYGVFS-----AMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLESVLGSK 71

Query: 67  EKSWESMVYSYRHGFSGFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLST 126
           EKS ES+VYSYRHGFSGFAAKLTNSQAQKLA M  V RV P+ LYKM+TTRSWDFLGLS+
Sbjct: 72  EKSLESIVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRTTRSWDFLGLSS 131

Query: 127 SPSQSSNLLHRGKMGEDVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFNSTN 186
           SPS SSNLLHR KMG++VIIGV+D+G WPESE+F D+GMGP+PSRWKG CESGE+FNS++
Sbjct: 132 SPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICESGEDFNSSH 191

Query: 187 CNRKIIGARWYNKGLIADGGQQALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGLGA 246
           CN+K+IGARW+   L+AD G++A+  +YLSARD QGHGTH ASTA G+FV NV+  G G 
Sbjct: 192 CNKKVIGARWFASALVADHGEEAVFMDYLSARDNQGHGTHTASTAGGAFVRNVSLFGNGR 251

Query: 247 GTVRGGAPRACLAIYKVLWSEG-AGSGADILMAMDDAIHDGVDVLSLSLRFGGVPISPEF 306
           GT+RGGAP A LAIYKVLWS+   GSGADIL  +D+AIHDGVDVLS+S+    +P+  + 
Sbjct: 252 GTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIG-KSIPLFSDV 311

Query: 307 SEDSVVAIGAFHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPV-TL 366
           +E + VA+G+FHA A+GISVVCAGGN+G  +QT+ N APW+FTVAASTIDRAFLA + TL
Sbjct: 312 NELNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLASITTL 371

Query: 367 GDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCKDILGNDTSLSGNIV-LCFNKLVDVET 426
           GDN T+LGQ+F   + DIVG L+    R    C  +LG++  +SGN+V LCF  L     
Sbjct: 372 GDNATYLGQTFL--KKDIVGMLLAMDGR----CAGLLGSNIHISGNVVLLCFTDLAKKAG 431

Query: 427 TQKAVVAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPMVRI 486
               V+  +QA   GII AGQH+DIL P   D PCI +D  VGTK F Y L      +R+
Sbjct: 432 ASNVVMPGKQAKVVGIIYAGQHNDILGP--CDTPCIHVDTHVGTKLFTYYLNDEKAFIRL 491

Query: 487 GRATTIVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKGFAF 546
               TI GKPIS+ +A+FSSRGPNS SP ILK PDIAAPGSNI+AA+ P +  SDKGFA 
Sbjct: 492 RATRTITGKPISSRIAYFSSRGPNSISPGILK-PDIAAPGSNIIAAVPPNHKGSDKGFAA 551

Query: 547 QSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKLADP 606
            SGTSMAAPHISGIVAL+KSL PTWSPAAI+SAL TTARV+ PSG PI AE SPPK+ADP
Sbjct: 552 MSGTSMAAPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKVADP 611

Query: 607 FDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSVL 666
           FDYGGG+V+ NAA DPGLIYDL   DY+ YYLC MGY  ++IS L++QKT CPS+R+SVL
Sbjct: 612 FDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEAEISHLSQQKTVCPSKRASVL 671

Query: 667 DLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTVKKI 726
           DLNLP+ITVPAL NSTTVTRTVTNVGN ++VY+AVI+AP G+KV V+P +LAFN  VKKI
Sbjct: 672 DLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLAFNSNVKKI 731

Query: 727 SFKVTISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTD 763
           SFKV  S+S+ RNYGY FGSL W+DG+H VK PLSVR D
Sbjct: 732 SFKVKFSSSLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 755

BLAST of MS026285 vs. NCBI nr
Match: XP_022931496.1 (subtilisin-like protease SBT3.7 isoform X4 [Cucurbita moschata])

HSP 1 Score: 958.0 bits (2475), Expect = 5.0e-275
Identity = 496/762 (65.09%), Postives = 594/762 (77.95%), Query Frame = 0

Query: 4   SHFIAAFVSFYALFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVL 63
           S F+ A V+ YA+FS     +MAEAD+QN KV+I YLGE  +ED KLTT++HHELL +V+
Sbjct: 7   SSFMHAVVTIYAVFS-----AMAEADNQNSKVYIAYLGERPYEDVKLTTDSHHELLESVM 66

Query: 64  GSKEKSWESMVYSYRHGFSGFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLG 123
           GSKEKS ESMVYSY+HGFSGFAAKLTNSQAQKLA M  V RV P+SLYKM TTRSWDFLG
Sbjct: 67  GSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWDFLG 126

Query: 124 LSTSPSQSSNLLHRGKMGEDVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFN 183
           LS+SPS SSNLLHR KMG++VIIGV+DSG WPESE+F D+GMGP+PSRWKG C+ GE+FN
Sbjct: 127 LSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGEDFN 186

Query: 184 STNCNRKIIGARWYNKGLIADGGQQALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQG 243
           S +CN+K+IGARW+ + LIAD G++A+  +YLSARD +GHGTH ASTA G+FV NV+Y G
Sbjct: 187 SFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFG 246

Query: 244 LGAGTVRGGAPRACLAIYKVLWSE-GAGSGADILMAMDDAIHDGVDVLSLSLRFGGVPIS 303
              GT+RGGAP A LAIYKV+WS+   GS ADIL  +D+AIHDGVDVLS+S+  G +P+ 
Sbjct: 247 NRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIG-GSIPLP 306

Query: 304 PEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPV 363
           P+ SE + VAIG+FHA ARG+SVVCAGGN+G  +QT+VN APW+FTVAASTIDRAFL  +
Sbjct: 307 PDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLTSI 366

Query: 364 -TLGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCKDILGNDTSLSGNIV-LCFNKLVD 423
            TLGDN T+LGQ+F   + D+VGKLV   RR    C   LG+D    GN+V LCF  L  
Sbjct: 367 TTLGDNATYLGQTFL--KKDVVGKLVVMDRR----CAGHLGSDIPTGGNVVLLCFIDLAM 426

Query: 424 VETTQKAVVAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPM 483
                  V+ V+QA   G+I AGQH DIL P   D PCI +D  VGTK   Y+L     +
Sbjct: 427 KAAASNPVMPVKQAKVVGVIYAGQHTDILGP--CDVPCIHVDTHVGTKLLTYILSDEKAL 486

Query: 484 VRIGRATTIVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKG 543
           +R+    TIVGKPIS+ +A+FSSRGPNS SP ILK PDIAAPGSNI+AA+ P +   DKG
Sbjct: 487 IRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILK-PDIAAPGSNIIAAVPPNHKGGDKG 546

Query: 544 FAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKL 603
           FA  SGTSMA PHISGIVAL+KSL PTWSPAAI+SAL TTARV+ PSG PI AE SPPK+
Sbjct: 547 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKV 606

Query: 604 ADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRS 663
           ADPFDYGGG+V+ NAA DPGLIYDL   DY+ YY+C MGY  ++IS L++QK  CPS+R+
Sbjct: 607 ADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRA 666

Query: 664 SVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTV 723
           SVLDLNLP+ITVPAL NSTTVTRTVTNVGN ++VY+AVI+AP G+KV V P +L FN  V
Sbjct: 667 SVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNV 726

Query: 724 KKISFKVTISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTD 763
           KKISFKV  S+++ RNYGY FGSL W+DG+H VK PLSVR D
Sbjct: 727 KKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 753

BLAST of MS026285 vs. NCBI nr
Match: XP_022931497.1 (subtilisin-like protease SBT3.7 isoform X5 [Cucurbita moschata])

HSP 1 Score: 957.6 bits (2474), Expect = 6.5e-275
Identity = 497/762 (65.22%), Postives = 593/762 (77.82%), Query Frame = 0

Query: 4   SHFIAAFVSFYALFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVL 63
           S F+ A V+ YA+FS     +MAEAD+QN KVHIVYLGE  ++D KLTT +HHELL +VL
Sbjct: 7   SSFMHAVVTIYAVFS-----AMAEADNQNSKVHIVYLGERPYDDVKLTTHSHHELLESVL 66

Query: 64  GSKEKSWESMVYSYRHGFSGFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLG 123
            SKEKS ESMVYSY+HGFSGFAAKLTNSQAQKLA M  V RV P+SLYKM TTRSWDFLG
Sbjct: 67  RSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWDFLG 126

Query: 124 LSTSPSQSSNLLHRGKMGEDVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFN 183
           LS+SPS SSNLLHR KMG++VIIGV+DSG WPESE+F D+GMGP+PSRWKG C+ GE+FN
Sbjct: 127 LSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGEDFN 186

Query: 184 STNCNRKIIGARWYNKGLIADGGQQALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQG 243
           S +CN+K+IGARW+ + LIAD G++A+  +YLSARD +GHGTH ASTA G+FV NV+Y G
Sbjct: 187 SFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFG 246

Query: 244 LGAGTVRGGAPRACLAIYKVLWSE-GAGSGADILMAMDDAIHDGVDVLSLSLRFGGVPIS 303
              GT+RGGAP A LAIYKV+WS+   GS ADIL  +D+AIHDGVDVLS+S+  G +P+ 
Sbjct: 247 NRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIG-GSIPLP 306

Query: 304 PEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPV 363
           P+ SE + VAIG+FHA ARG+SVVCAGGN+G  +QT+VN APW+FTVAASTIDRAFL  +
Sbjct: 307 PDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLTSI 366

Query: 364 -TLGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCKDILGNDTSLSGNIV-LCFNKLVD 423
            TLGDN T+LGQ+F   + D+VGKLV   RR    C   LG+D    GN+V LCF  L  
Sbjct: 367 TTLGDNATYLGQTFL--KKDVVGKLVVMDRR----CAGHLGSDIPTGGNVVLLCFIDLAM 426

Query: 424 VETTQKAVVAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPM 483
                  V+ V+QA   G+I AGQH DIL P   D PCI +D  VGTK   Y+L     +
Sbjct: 427 KAAASNPVMPVKQAKVVGVIYAGQHTDILGP--CDVPCIHVDTHVGTKLLTYILSDEKAL 486

Query: 484 VRIGRATTIVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKG 543
           +R+    TIVGKPIS+ +A+FSSRGPNS SP ILK PDIAAPGSNI+AA+ P +   DKG
Sbjct: 487 IRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILK-PDIAAPGSNIIAAVPPNHKGGDKG 546

Query: 544 FAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKL 603
           FA  SGTSMA PHISGIVAL+KSL PTWSPAAI+SAL TTARV+ PSG PI AE SPPK+
Sbjct: 547 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKV 606

Query: 604 ADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRS 663
           ADPFDYGGG+V+ NAA DPGLIYDL   DY+ YY+C MGY  ++IS L++QK  CPS+R+
Sbjct: 607 ADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRA 666

Query: 664 SVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTV 723
           SVLDLNLP+ITVPAL NSTTVTRTVTNVGN ++VY+AVI+AP G+KV V P +L FN  V
Sbjct: 667 SVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNV 726

Query: 724 KKISFKVTISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTD 763
           KKISFKV  S+++ RNYGY FGSL W+DG+H VK PLSVR D
Sbjct: 727 KKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 753

BLAST of MS026285 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 1.2e-220
Identity = 402/756 (53.17%), Postives = 516/756 (68.25%), Query Frame = 0

Query: 23  KSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVLGSKEKSWESMVYSYRHGFS 82
           +S        RKVHIVYLGE QH+D +  TE+HH +L ++LGSKE + +SMVYSYRHGFS
Sbjct: 22  RSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFS 81

Query: 83  GFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGE 142
           GFAAKLT SQA+K+A++  VV V+P+S YK+ TTR+WD+LGLS +  +S  LLH   MGE
Sbjct: 82  GFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS--LLHETNMGE 141

Query: 143 DVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGLI 202
            +IIGV+D+G+WPESE F D G GPVPS WKG CE+GE FNS+NCN+K+IGA+++  G +
Sbjct: 142 QIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFL 201

Query: 203 ADGGQQALAN--EYLSARDFQGHGTHVASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAI 262
           A+       N  +++S RD  GHGTHV++ A GSFVPN++Y+GL  GTVRGGAPRA +A+
Sbjct: 202 AENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAM 261

Query: 263 YKVLW-----SEGAGSGADILMAMDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGA 322
           YK  W          S ADIL AMD+A+HDGVDVLS+SL    VP+  E      +  GA
Sbjct: 262 YKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLG-SSVPLYGETDIRDGITTGA 321

Query: 323 FHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSF 382
           FHA  +GI+VVC+GGN GPD  T+ NTAPWI TVAA+T+DR+F  P+TLG+N   LGQ+ 
Sbjct: 322 FHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQAM 381

Query: 383 FAGENDIVGKLVC------STRRRLCSCKDILGN-DTSLSGNIVLCFNKLVDVETTQKAV 442
           + G       LV       S      +C+++L N + ++ G +VLCF           A 
Sbjct: 382 YTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPYGGAVLSAA 441

Query: 443 VAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPMVRIGRATT 502
             V++A G G+I+A      + P   DFPC+ +D ++GT    Y   + +P+V+I  + T
Sbjct: 442 RYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKT 501

Query: 503 IVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKGFAFQSGTS 562
           +VG+P+ T VA FSSRGPNS +PAILK PDIAAPG +ILAA +    FSD+GF   SGTS
Sbjct: 502 LVGQPVGTKVATFSSRGPNSIAPAILK-PDIAAPGVSILAA-TTNTTFSDQGFIMLSGTS 561

Query: 563 MAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKLADPFDYGG 622
           MAAP ISG+ ALLK+LH  WSPAAIRSA+ TTA    P G  I AE SPPKLADPFDYGG
Sbjct: 562 MAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGG 621

Query: 623 GLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSVLDLNLP 682
           GLVNP  +A+PGL+YD+ + DYV  Y+C +GY  + ISQL  + T C + + SVLD NLP
Sbjct: 622 GLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLP 681

Query: 683 SITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTVKKISFKVT 742
           SIT+P L++  T+TRTVTNVG  +SVYR  +  P G +V V P  L FN T KK+ FKV 
Sbjct: 682 SITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVK 741

Query: 743 ISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTDLL 765
           +S +   N GY FGSL WSD LH+V IPLSVRT +L
Sbjct: 742 VSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 771

BLAST of MS026285 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 6.2e-220
Identity = 404/745 (54.23%), Postives = 512/745 (68.72%), Query Frame = 0

Query: 34  KVHIVYLGETQHEDSKLTTETHHELLATVLGSKEKSWESMVYSYRHGFSGFAAKLTNSQA 93
           KVHIVYLGE QH+D +  TE+HH +L ++LGSKE +  SMV+SYRHGFSGFAAKLT SQA
Sbjct: 29  KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQA 88

Query: 94  QKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGEDVIIGVLDSGI 153
           +KLA++  VV V P+S Y++ TTR+WD+LGLS   +   NLL+   MGE+VIIG++DSG+
Sbjct: 89  KKLADLPEVVHVTPDSFYQLDTTRTWDYLGLSV--ANPKNLLNDTNMGEEVIIGIVDSGV 148

Query: 154 WPESEAFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGLIA--DGGQQALA 213
           WPESE F D G+GPVPS WKG C SGE F S+ CN+K+IGA+++  G +A  +      +
Sbjct: 149 WPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQCNKKLIGAKYFINGFLATHESFNSTES 208

Query: 214 NEYLSARDFQGHGTHVASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLW-----S 273
            +++S RD  GHGTHVA+ A GS+VP+++Y+GL  GTVRGGAPRA +A+YK  W      
Sbjct: 209 LDFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLDRFD 268

Query: 274 EGAGSGADILMAMDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVV 333
               S ADIL AMD+A+HDGVDVLSLS+ +   P  PE    +V+A GAFHA  +GI+VV
Sbjct: 269 INTCSSADILKAMDEAMHDGVDVLSLSIGY-RFPYFPETDVRAVIATGAFHAVLKGITVV 328

Query: 334 CAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSFFAGENDIVGKL 393
           C+GGN GP  QT+ NTAPWI TVAA+T+DR+F  P+TLG+N   LGQ+ + G       L
Sbjct: 329 CSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILGQAMYTGPELGFTSL 388

Query: 394 VC------STRRRLCSCKDILGNDT-SLSGNIVLCFNKLVDVETTQKAVVAVRQANGNGI 453
           V       S       C+ +  N   +++G +VLCF       T   AV  V++A G G+
Sbjct: 389 VYPENPGNSNESFSGDCELLFFNSNHTMAGKVVLCFTTSTRYITVSSAVSYVKEAGGLGV 448

Query: 454 IVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPMVRIGRATTIVGKPISTNVA 513
           IVA    D L P   DFPC+ +D ++GT    Y+  T  P+V+I  + T+VG+P+ T VA
Sbjct: 449 IVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQPSKTLVGQPVGTKVA 508

Query: 514 FFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVA 573
            FSSRGPNS  PAILK PDIAAPG +ILAA +    F+D+GF F SGTSMAAP ISG+VA
Sbjct: 509 DFSSRGPNSIEPAILK-PDIAAPGVSILAATTTNKTFNDRGFIFLSGTSMAAPTISGVVA 568

Query: 574 LLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADP 633
           LLK+LH  WSPAAIRSA+ TTA    P G  I AE SP KLADPFDYGGGLVNP  AA P
Sbjct: 569 LLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKLADPFDYGGGLVNPEKAAKP 628

Query: 634 GLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNST 693
           GL+YDL + DYV  Y+C +GY  + ISQL  + T C + + SVLD NLPSIT+P L++  
Sbjct: 629 GLVYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSNPKPSVLDFNLPSITIPNLKDEV 688

Query: 694 TVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTVKKISFKVTISASIHRNYGY 753
           T+TRT+TNVG   SVY+ VI  P G +V V P  L FN T K++SFKV +S +   N GY
Sbjct: 689 TLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKVKVSTTHKINTGY 748

Query: 754 LFGSLIWSDGLHHVKIPLSVRTDLL 765
            FGSL WSD LH+V IPLSVRT +L
Sbjct: 749 FFGSLTWSDSLHNVTIPLSVRTQIL 768

BLAST of MS026285 vs. ExPASy Swiss-Prot
Match: Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 764.6 bits (1973), Expect = 1.1e-219
Identity = 400/744 (53.76%), Postives = 517/744 (69.49%), Query Frame = 0

Query: 34  KVHIVYLGETQHEDSKLTTETHHELLATVLGSKEKSWESMVYSYRHGFSGFAAKLTNSQA 93
           KVHIVYLGE QH+D +  TE+HH +L ++LGSKE++  SMV+S+RHGFSGFAAKLT SQA
Sbjct: 33  KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQA 92

Query: 94  QKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGEDVIIGVLDSGI 153
           +K+A++  VV V+P+  YK  TTR+WD+LGL  SP+   NLL++  MGE +IIG++DSG+
Sbjct: 93  KKIADLPEVVHVIPDRFYKPATTRTWDYLGL--SPTNPKNLLNQTNMGEQMIIGIIDSGV 152

Query: 154 WPESEAFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGLIA--DGGQQALA 213
           WPESE F D  +GPVPS WKG CESGE+FNS++CN+K+IGA+++    +A  +    + +
Sbjct: 153 WPESEVFNDNEIGPVPSHWKGGCESGEDFNSSHCNKKLIGAKYFINAFLATHESFNSSES 212

Query: 214 NEYLSARDFQGHGTHVASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLW----SE 273
            +++S R + GHGTHVA+ A GS+VPN +Y+GL  GTVRGGAPRA +A+YK  W      
Sbjct: 213 LDFISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLDI 272

Query: 274 GAGSGADILMAMDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVVC 333
            A S ADIL AMD+AIHDGVDVLSLSL F   P+ PE      +A GAFHA  +GI+VVC
Sbjct: 273 AACSSADILKAMDEAIHDGVDVLSLSLGF--EPLYPETDVRDGIATGAFHAVLKGITVVC 332

Query: 334 AGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSFFAGENDIVGKLV 393
           A GN GP  QT+ NTAPWI TVAA+T+DR+F+ P+TLG+N   LGQ+ + G       LV
Sbjct: 333 AAGNAGPAAQTVGNTAPWILTVAATTLDRSFVTPMTLGNNKVILGQAIYTGTEVGFTSLV 392

Query: 394 C------STRRRLCSCKDILGN-DTSLSGNIVLCFNKLVDVETTQKAVVAVRQANGNGII 453
                  S      +C+ +L N + +++G +VLCF +     +  +A   V++A G G+I
Sbjct: 393 YPENPGNSNESFSGTCERLLINSNRTMAGKVVLCFTESPYSISVTRAAHYVKRAGGLGVI 452

Query: 454 VAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPMVRIGRATTIVGKPISTNVAF 513
           +AGQ  ++L P   DFPC+ +D ++GT    Y+    +P+V+I  + T++G+P+ T VA 
Sbjct: 453 IAGQPGNVLRPCLDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVGTKVAS 512

Query: 514 FSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVAL 573
           FSSRGPN  S AILK PDIAAPG +ILAA +    F+D+GF F SGTSMA P ISGIVAL
Sbjct: 513 FSSRGPNPISAAILK-PDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISGIVAL 572

Query: 574 LKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPG 633
           LK+LHP WSPAAIRSA+ TTA    P G  I AE SP K ADPFDYGGGLVNP  A  PG
Sbjct: 573 LKALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKATKPG 632

Query: 634 LIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNSTT 693
           L+YDL + DYV  Y+C +GY  + ISQL  + T C   + SVLD NLPSIT+P L+   T
Sbjct: 633 LVYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKEEVT 692

Query: 694 VTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTVKKISFKVTISASIHRNYGYL 753
           + RT+TNVG   SVYR  +  P GT+V V P  L FN T K++SFKV++S +   N GY 
Sbjct: 693 LPRTLTNVGPLESVYRVAVEPPLGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINTGYY 752

Query: 754 FGSLIWSDGLHHVKIPLSVRTDLL 765
           FGSL WSD LH+V IPLSVRT LL
Sbjct: 753 FGSLTWSDSLHNVTIPLSVRTQLL 770

BLAST of MS026285 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 4.4e-218
Identity = 407/767 (53.06%), Postives = 524/767 (68.32%), Query Frame = 0

Query: 12  SFYALFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVLGSKEKSWE 71
           S   + S+    ++A A  ++ KVHIVYLGE QH+D K  TE+HH++L+++LGSK+ + E
Sbjct: 7   SVLVVLSLIIVLNVARASAKS-KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHE 66

Query: 72  SMVYSYRHGFSGFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQS 131
           SMVYSYRHGFSGFAAKLT SQA+K+A+   V+ V+P+S Y++ TTR WD+LG   S   S
Sbjct: 67  SMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYELATTRIWDYLG--PSADNS 126

Query: 132 SNLLHRGKMGEDVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKI 191
            NL+    MG+  IIGV+D+G+WPESE+F D G+GPVPS WKG CE GE F STNCNRK+
Sbjct: 127 KNLVSDTNMGDQTIIGVIDTGVWPESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKL 186

Query: 192 IGARWYNKGLIADGGQQALAN-EYLSARDFQGHGTHVASTAAGSFVPNVNYQGLGAGTVR 251
           IGA+++  G +A+    A  + +Y+SARDF GHGTHVAS A GSFVPNV+Y+GLG GT+R
Sbjct: 187 IGAKYFINGFLAENQFNATESPDYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLR 246

Query: 252 GGAPRACLAIYKVLW--SEGAG---SGADILMAMDDAIHDGVDVLSLSLRFGGVPISPEF 311
           GGAPRA +A+YK  W  +E  G   S +DI+ A+D+AIHDGVDVLS+SL  G VP++ E 
Sbjct: 247 GGAPRARIAMYKACWYINELDGVTCSFSDIMKAIDEAIHDGVDVLSISLG-GRVPLNSET 306

Query: 312 SEDSVVAIGAFHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPVTLG 371
                +A GAFHA A+GI VVCAGGN GP  QT+VNTAPWI TVAA+T+DR+F  P+ LG
Sbjct: 307 DLRDGIATGAFHAVAKGIVVVCAGGNAGPSSQTVVNTAPWILTVAATTLDRSFATPIILG 366

Query: 372 DNTTHLGQSFFAGENDIVGKLV--------CSTRRRLCSCKDILGNDTSLSGNIVLCFNK 431
           +N   LGQ+ + G       LV          T   +C   ++  N T ++G +VLCF  
Sbjct: 367 NNQVILGQAMYIGPELGFTSLVYPEDPGNSIDTFSGVCESLNLNSNRT-MAGKVVLCFTT 426

Query: 432 LVDVETTQKAVVAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTS 491
             D      A   V+ A G G+I+A      L P   DFPC+ +D ++GT    Y+  T 
Sbjct: 427 ARDFTVVSTAASIVKAAGGLGLIIARNPGYNLAPCSDDFPCVAIDNELGTDILFYIRYTG 486

Query: 492 NPMVRIGRATTIVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFS 551
           +P+V+I  + T+VG+P+ T VA FSSRGPNS SPAILK PDIAAPG +ILAA SP +  +
Sbjct: 487 SPVVKIQPSRTLVGEPVGTKVATFSSRGPNSISPAILK-PDIAAPGVSILAATSPNDTLN 546

Query: 552 DKGFAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSP 611
             GF  +SGTSMAAP ISG++ALLKSLHP WSPAA RSA+ TTA    P G  I AESS 
Sbjct: 547 AGGFVMRSGTSMAAPVISGVIALLKSLHPDWSPAAFRSAIVTTAWRTDPFGEQIAAESSS 606

Query: 612 PKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPS 671
            K+ DPFDYGGGLVNP  AA+PGLI D+   DYV  YLC  GY +S IS+L  + T C +
Sbjct: 607 LKVPDPFDYGGGLVNPEKAAEPGLILDMDSQDYV-LYLCSAGYNDSSISRLVGKVTVCSN 666

Query: 672 QRSSVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFN 731
            + SVLD+NLPSIT+P L++  T+TRTVTNVG   SVY+ ++  P G +V V P  L FN
Sbjct: 667 PKPSVLDINLPSITIPNLKDEVTLTRTVTNVGPVDSVYKVLVEPPLGIQVVVTPETLVFN 726

Query: 732 PTVKKISFKVTISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTDLL 765
              K +SF V +S +   N G+ FGSL W+D +H+V IP+SVRT +L
Sbjct: 727 SKTKSVSFTVIVSTTHKINTGFYFGSLTWTDSIHNVVIPVSVRTQIL 766

BLAST of MS026285 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 7.1e-216
Identity = 398/764 (52.09%), Postives = 522/764 (68.32%), Query Frame = 0

Query: 16  LFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVLGSKEKSWESMVY 75
           + S+    ++  A D++ KVHIVYLGE QH+D +  +E+HH++L+++LGSK  + ESMVY
Sbjct: 11  VLSLVIVLNVVRASDES-KVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVY 70

Query: 76  SYRHGFSGFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLL 135
           SYRHGFSGFAAKLT SQA+KLA+   VV V+ +S Y++ TTR+WD+LGLS   +  +NLL
Sbjct: 71  SYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLSV--ANPNNLL 130

Query: 136 HRGKMGEDVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGAR 195
           +   MG+ VIIG +D+G+WPESE+F D G+GP+PS WKG CESGE+F STNCNRK+IGA+
Sbjct: 131 NDTNMGDQVIIGFIDTGVWPESESFNDNGVGPIPSHWKGGCESGEKFISTNCNRKLIGAK 190

Query: 196 WYNKGLIA--DGGQQALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGLGAGTVRGGA 255
           ++  G +A  +G     + +Y+SARDF GHGTH AS A GSFVPN++Y+GL  G +RGGA
Sbjct: 191 YFINGFLAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGGA 250

Query: 256 PRACLAIYKVLW-----SEGAGSGADILMAMDDAIHDGVDVLSLSLRFGGVPISPEFSED 315
           PRA +AIYK  W        A S +DIL AMD+++HDGVDVLSLSL    +P+ PE    
Sbjct: 251 PRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLG-AQIPLYPETDLR 310

Query: 316 SVVAIGAFHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPVTLGDNT 375
             +A GAFHA A+GI VVCAGGN GP  QT++NTAPWI TVAA+T+DR+F  P+TLG+  
Sbjct: 311 DRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGNRK 370

Query: 376 THLGQSFFAGENDIVGKLVC--------STRRRLCSCKDILGNDTSLSGNIVLCFNKLVD 435
             LGQ+ + G+      LV          T   +C   ++  N T ++G +VLCF     
Sbjct: 371 VILGQALYTGQELGFTSLVYPENAGFTNETFSGVCERLNLNPNRT-MAGKVVLCFTTNTL 430

Query: 436 VETTQKAVVAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPM 495
                +A   V+ A G G+I+A      L P   DFPC+ +D ++GT    Y+  T +P+
Sbjct: 431 FTAVSRAASYVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRSTRSPV 490

Query: 496 VRIGRATTIVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKG 555
           V+I  + T+VG+P+ T VA FSSRGPNS SPAILK PDI APG +ILAA SP +  S  G
Sbjct: 491 VKIQPSRTLVGQPVGTKVATFSSRGPNSISPAILK-PDIGAPGVSILAATSPDSNSSVGG 550

Query: 556 FAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKL 615
           F   +GTSMAAP ++G+VALLK+LHP WSPAA RSA+ TTA    P G  I AE S  K+
Sbjct: 551 FDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKV 610

Query: 616 ADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRS 675
           ADPFDYGGG+VNP  AADPGLIYD+   DY+  YLC  GY +S I+QL    T C + ++
Sbjct: 611 ADPFDYGGGIVNPEKAADPGLIYDMGPRDYI-LYLCSAGYNDSSITQLVGNVTVCSTPKT 670

Query: 676 SVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTV 735
           SVLD+NLPSIT+P L++  T+TRTVTNVG   SVY+ V+  P G +V V P  L FN   
Sbjct: 671 SVLDVNLPSITIPDLKDEVTLTRTVTNVGTVDSVYKVVVEPPLGIQVVVAPETLVFNSKT 730

Query: 736 KKISFKVTISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTDLL 765
           K +SF V +S +   N G+ FG+LIW+D +H+V IP+SVRT +L
Sbjct: 731 KNVSFTVRVSTTHKINTGFYFGNLIWTDSMHNVTIPVSVRTQIL 767

BLAST of MS026285 vs. ExPASy TrEMBL
Match: A0A6J1E3R4 (subtilisin-like protease SBT3.4 OS=Momordica charantia OX=3673 GN=LOC111025797 PE=3 SV=1)

HSP 1 Score: 1166.0 bits (3015), Expect = 0.0e+00
Identity = 589/603 (97.68%), Postives = 590/603 (97.84%), Query Frame = 0

Query: 99  MAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGEDVIIGVLDSGIWPESE 158
           MAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGE VIIGVLDSGIWPESE
Sbjct: 1   MAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGEHVIIGVLDSGIWPESE 60

Query: 159 AFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGLIADGGQQALANEYLSAR 218
           AFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKG IAD GQ ALANEY SAR
Sbjct: 61  AFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGXIADXGQXALANEYXSAR 120

Query: 219 DFQGHGTHVASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLWSEGAGSGADILMA 278
           DF GHGTH ASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLWSEGAGSGADILMA
Sbjct: 121 DFXGHGTHXASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLWSEGAGSGADILMA 180

Query: 279 MDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQT 338
           MDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQT
Sbjct: 181 MDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQT 240

Query: 339 IVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCK 398
           IVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCK
Sbjct: 241 IVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCK 300

Query: 399 DILGNDTSLSGNIVLCFNKLVDVETTQKAVVAVRQANGNGIIVAGQHDDILFPYGADFPC 458
           DILGNDTSLSGNIVLCFNKLVD+E TQKAVVAVRQANGNGIIVAGQHDDILFP GADFPC
Sbjct: 301 DILGNDTSLSGNIVLCFNKLVDLEMTQKAVVAVRQANGNGIIVAGQHDDILFPCGADFPC 360

Query: 459 IVLDPDVGTKFFHYLLGTSNPMVRIGRATTIVGKPISTNVAFFSSRGPNSASPAILKQPD 518
           IVLDPDVGTKFFHY L TSNPMVRIGRA TIVGKPISTNVAFFSSRGPNSASPAILK PD
Sbjct: 361 IVLDPDVGTKFFHYXLDTSNPMVRIGRAXTIVGKPISTNVAFFSSRGPNSASPAILK-PD 420

Query: 519 IAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALS 578
           IAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALS
Sbjct: 421 IAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALS 480

Query: 579 TTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGM 638
           TTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGM
Sbjct: 481 TTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGM 540

Query: 639 GYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAV 698
           GYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAV
Sbjct: 541 GYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAV 600

Query: 699 IRA 702
           IRA
Sbjct: 601 IRA 602

BLAST of MS026285 vs. ExPASy TrEMBL
Match: A0A6J1JBL4 (subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 5.2e-278
Identity = 498/760 (65.53%), Postives = 595/760 (78.29%), Query Frame = 0

Query: 6   FIAAFVSFYALFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVLGS 65
           FI A V+ YA+FS      MAEADDQN KVHIVYLGE  ++D KLTT++HHELL +VLGS
Sbjct: 9   FILAVVTIYAVFS-----DMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLESVLGS 68

Query: 66  KEKSWESMVYSYRHGFSGFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLS 125
           KEKS ESMVYSYRHGFSGFAAKLTNSQAQKLA M  V RV P+SLYKM+TTRSWDFLGLS
Sbjct: 69  KEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWDFLGLS 128

Query: 126 TSPSQSSNLLHRGKMGEDVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFNST 185
           +SPS+SSNL HR KMG++VIIGV+D+G WPESE+F D+GMGP+PSRWKG C+SGE+FNS+
Sbjct: 129 SSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDFNSS 188

Query: 186 NCNRKIIGARWYNKGLIADGGQQALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGLG 245
           +CN+K+IGARW+   LIAD G++A+  +YLSARD +GHGTH ASTA G+FV NV+Y G G
Sbjct: 189 HCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVRNVSYFGNG 248

Query: 246 AGTVRGGAPRACLAIYKVLWSEG-AGSGADILMAMDDAIHDGVDVLSLSLRFGGVPISPE 305
            GT+RGGAP A LAIYKVLWS+   GSGADIL  +D+AIHDGVDVLS+S+    +P+ P+
Sbjct: 249 RGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIG-KSIPLFPD 308

Query: 306 FSEDSVVAIGAFHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPV-T 365
            ++ + VA+G+FHA A+GISVVCAGGN+G  +QT+ N APW+FTVAASTIDRAFL  + T
Sbjct: 309 VNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLVSITT 368

Query: 366 LGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCKDILGNDTSLSGNIV-LCFNKLVDVE 425
           LGDN T+LGQ F     DIVG LV    R    C  ILG D  +SGN+V LCF  L    
Sbjct: 369 LGDNATYLGQMFL--RKDIVGMLVAMDGR----CAGILGGDIPISGNVVLLCFTDLAKTA 428

Query: 426 TTQKAVVAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPMVR 485
               AV+  +QA   G+I AGQ  D L P   D PCI +D  VGTK F YLL   + ++R
Sbjct: 429 PASNAVMPGKQAKVVGVIYAGQQTDNLVP--CDIPCIHVDTHVGTKLFTYLLNDDDALIR 488

Query: 486 IGRATTIVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKGFA 545
           +    TI+GKPIS+ +A+FSSRGPNS SP ILK PDIAAPG+NI+AA+ P +   DKGFA
Sbjct: 489 LRATRTIIGKPISSRIAYFSSRGPNSFSPGILK-PDIAAPGANIIAAVPPNHKGGDKGFA 548

Query: 546 FQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKLAD 605
             SGTSMA PHISGIV L+KSLHPTWSPAAI+SAL TTARV+ PSG PI AE SPPK+AD
Sbjct: 549 TMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSPPKVAD 608

Query: 606 PFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSV 665
           PFDYGGG+V+ NAA DPGLIYDL   DY+ YYLC MGY  +DIS L++QKT CPS+R+S+
Sbjct: 609 PFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASI 668

Query: 666 LDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTVKK 725
           LDLNLP+ITVPAL NSTTVTRTVTNVGN ++VY+AVI+AP G+KV V+P +L FN  VKK
Sbjct: 669 LDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK 728

Query: 726 ISFKVTISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTD 763
           ISFKV  S+++ RNYGY FGSL W+DG+H VK PLSVR D
Sbjct: 729 ISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 753

BLAST of MS026285 vs. ExPASy TrEMBL
Match: A0A6J1ETT0 (subtilisin-like protease SBT3.7 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 2.4e-275
Identity = 496/762 (65.09%), Postives = 594/762 (77.95%), Query Frame = 0

Query: 4   SHFIAAFVSFYALFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVL 63
           S F+ A V+ YA+FS     +MAEAD+QN KV+I YLGE  +ED KLTT++HHELL +V+
Sbjct: 7   SSFMHAVVTIYAVFS-----AMAEADNQNSKVYIAYLGERPYEDVKLTTDSHHELLESVM 66

Query: 64  GSKEKSWESMVYSYRHGFSGFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLG 123
           GSKEKS ESMVYSY+HGFSGFAAKLTNSQAQKLA M  V RV P+SLYKM TTRSWDFLG
Sbjct: 67  GSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWDFLG 126

Query: 124 LSTSPSQSSNLLHRGKMGEDVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFN 183
           LS+SPS SSNLLHR KMG++VIIGV+DSG WPESE+F D+GMGP+PSRWKG C+ GE+FN
Sbjct: 127 LSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGEDFN 186

Query: 184 STNCNRKIIGARWYNKGLIADGGQQALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQG 243
           S +CN+K+IGARW+ + LIAD G++A+  +YLSARD +GHGTH ASTA G+FV NV+Y G
Sbjct: 187 SFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFG 246

Query: 244 LGAGTVRGGAPRACLAIYKVLWSE-GAGSGADILMAMDDAIHDGVDVLSLSLRFGGVPIS 303
              GT+RGGAP A LAIYKV+WS+   GS ADIL  +D+AIHDGVDVLS+S+  G +P+ 
Sbjct: 247 NRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIG-GSIPLP 306

Query: 304 PEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPV 363
           P+ SE + VAIG+FHA ARG+SVVCAGGN+G  +QT+VN APW+FTVAASTIDRAFL  +
Sbjct: 307 PDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLTSI 366

Query: 364 -TLGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCKDILGNDTSLSGNIV-LCFNKLVD 423
            TLGDN T+LGQ+F   + D+VGKLV   RR    C   LG+D    GN+V LCF  L  
Sbjct: 367 TTLGDNATYLGQTFL--KKDVVGKLVVMDRR----CAGHLGSDIPTGGNVVLLCFIDLAM 426

Query: 424 VETTQKAVVAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPM 483
                  V+ V+QA   G+I AGQH DIL P   D PCI +D  VGTK   Y+L     +
Sbjct: 427 KAAASNPVMPVKQAKVVGVIYAGQHTDILGP--CDVPCIHVDTHVGTKLLTYILSDEKAL 486

Query: 484 VRIGRATTIVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKG 543
           +R+    TIVGKPIS+ +A+FSSRGPNS SP ILK PDIAAPGSNI+AA+ P +   DKG
Sbjct: 487 IRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILK-PDIAAPGSNIIAAVPPNHKGGDKG 546

Query: 544 FAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKL 603
           FA  SGTSMA PHISGIVAL+KSL PTWSPAAI+SAL TTARV+ PSG PI AE SPPK+
Sbjct: 547 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKV 606

Query: 604 ADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRS 663
           ADPFDYGGG+V+ NAA DPGLIYDL   DY+ YY+C MGY  ++IS L++QK  CPS+R+
Sbjct: 607 ADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRA 666

Query: 664 SVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTV 723
           SVLDLNLP+ITVPAL NSTTVTRTVTNVGN ++VY+AVI+AP G+KV V P +L FN  V
Sbjct: 667 SVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNV 726

Query: 724 KKISFKVTISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTD 763
           KKISFKV  S+++ RNYGY FGSL W+DG+H VK PLSVR D
Sbjct: 727 KKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 753

BLAST of MS026285 vs. ExPASy TrEMBL
Match: A0A6J1ETU3 (subtilisin-like protease SBT3.7 isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 3.1e-275
Identity = 497/762 (65.22%), Postives = 593/762 (77.82%), Query Frame = 0

Query: 4   SHFIAAFVSFYALFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVL 63
           S F+ A V+ YA+FS     +MAEAD+QN KVHIVYLGE  ++D KLTT +HHELL +VL
Sbjct: 7   SSFMHAVVTIYAVFS-----AMAEADNQNSKVHIVYLGERPYDDVKLTTHSHHELLESVL 66

Query: 64  GSKEKSWESMVYSYRHGFSGFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLG 123
            SKEKS ESMVYSY+HGFSGFAAKLTNSQAQKLA M  V RV P+SLYKM TTRSWDFLG
Sbjct: 67  RSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWDFLG 126

Query: 124 LSTSPSQSSNLLHRGKMGEDVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFN 183
           LS+SPS SSNLLHR KMG++VIIGV+DSG WPESE+F D+GMGP+PSRWKG C+ GE+FN
Sbjct: 127 LSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGEDFN 186

Query: 184 STNCNRKIIGARWYNKGLIADGGQQALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQG 243
           S +CN+K+IGARW+ + LIAD G++A+  +YLSARD +GHGTH ASTA G+FV NV+Y G
Sbjct: 187 SFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFG 246

Query: 244 LGAGTVRGGAPRACLAIYKVLWSE-GAGSGADILMAMDDAIHDGVDVLSLSLRFGGVPIS 303
              GT+RGGAP A LAIYKV+WS+   GS ADIL  +D+AIHDGVDVLS+S+  G +P+ 
Sbjct: 247 NRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIG-GSIPLP 306

Query: 304 PEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPV 363
           P+ SE + VAIG+FHA ARG+SVVCAGGN+G  +QT+VN APW+FTVAASTIDRAFL  +
Sbjct: 307 PDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLTSI 366

Query: 364 -TLGDNTTHLGQSFFAGENDIVGKLVCSTRRRLCSCKDILGNDTSLSGNIV-LCFNKLVD 423
            TLGDN T+LGQ+F   + D+VGKLV   RR    C   LG+D    GN+V LCF  L  
Sbjct: 367 TTLGDNATYLGQTFL--KKDVVGKLVVMDRR----CAGHLGSDIPTGGNVVLLCFIDLAM 426

Query: 424 VETTQKAVVAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPM 483
                  V+ V+QA   G+I AGQH DIL P   D PCI +D  VGTK   Y+L     +
Sbjct: 427 KAAASNPVMPVKQAKVVGVIYAGQHTDILGP--CDVPCIHVDTHVGTKLLTYILSDEKAL 486

Query: 484 VRIGRATTIVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKG 543
           +R+    TIVGKPIS+ +A+FSSRGPNS SP ILK PDIAAPGSNI+AA+ P +   DKG
Sbjct: 487 IRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILK-PDIAAPGSNIIAAVPPNHKGGDKG 546

Query: 544 FAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKL 603
           FA  SGTSMA PHISGIVAL+KSL PTWSPAAI+SAL TTARV+ PSG PI AE SPPK+
Sbjct: 547 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKV 606

Query: 604 ADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRS 663
           ADPFDYGGG+V+ NAA DPGLIYDL   DY+ YY+C MGY  ++IS L++QK  CPS+R+
Sbjct: 607 ADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRA 666

Query: 664 SVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTV 723
           SVLDLNLP+ITVPAL NSTTVTRTVTNVGN ++VY+AVI+AP G+KV V P +L FN  V
Sbjct: 667 SVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNV 726

Query: 724 KKISFKVTISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTD 763
           KKISFKV  S+++ RNYGY FGSL W+DG+H VK PLSVR D
Sbjct: 727 KKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 753

BLAST of MS026285 vs. ExPASy TrEMBL
Match: A0A6J1EZK4 (subtilisin-like protease SBT3.7 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 9.1e-275
Identity = 495/757 (65.39%), Postives = 589/757 (77.81%), Query Frame = 0

Query: 9   AFVSFYALFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVLGSKEK 68
           A V+ YA+FS     +MAE DDQN KVHIVYLGE  ++D KLTT +HHELL +VL SKEK
Sbjct: 14  AIVTIYAVFS-----AMAEVDDQNSKVHIVYLGERPYDDVKLTTHSHHELLESVLRSKEK 73

Query: 69  SWESMVYSYRHGFSGFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLSTSP 128
           S ESMVYSY+HGFSGFAAKLTNSQAQKLA M  V RV P+SLYKM TTRSWDFLGLS+SP
Sbjct: 74  SLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWDFLGLSSSP 133

Query: 129 SQSSNLLHRGKMGEDVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFNSTNCN 188
           S SSNLLHR KMG++VIIGV+DSG WPESE+F D+GMGP+PSRWKG C+ GE+FNS +CN
Sbjct: 134 SASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGEDFNSFHCN 193

Query: 189 RKIIGARWYNKGLIADGGQQALANEYLSARDFQGHGTHVASTAAGSFVPNVNYQGLGAGT 248
           +K+IGARW+ + LIAD G++A+  +YLSARD +GHGTH ASTA G+FV NV+Y G   GT
Sbjct: 194 KKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNRRGT 253

Query: 249 VRGGAPRACLAIYKVLWSE-GAGSGADILMAMDDAIHDGVDVLSLSLRFGGVPISPEFSE 308
           +RGGAP A LAIYKV+WS+   GS ADIL  +D+AIHDGVDVLS+S+  G +P+ P+ SE
Sbjct: 254 LRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIG-GSIPLPPDVSE 313

Query: 309 DSVVAIGAFHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPV-TLGD 368
            + VAIG+FHA ARG+SVVCAGGN+G  +QT+VN APW+FTVAASTIDRAFL  + TLGD
Sbjct: 314 WNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLTSITTLGD 373

Query: 369 NTTHLGQSFFAGENDIVGKLVCSTRRRLCSCKDILGNDTSLSGNIV-LCFNKLVDVETTQ 428
           N T+LGQ+F   + D+VGKLV   RR    C   LG+D    GN+V LCF  L       
Sbjct: 374 NATYLGQTFL--KKDVVGKLVVMDRR----CAGHLGSDIPTGGNVVLLCFIDLAMKAAAS 433

Query: 429 KAVVAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPMVRIGR 488
             V+ V+QA   G+I AGQH DIL P   D PCI +D  VGTK   Y+L     ++R+  
Sbjct: 434 NPVMPVKQAKVVGVIYAGQHTDILGP--CDVPCIHVDTHVGTKLLTYILSDEKALIRLKA 493

Query: 489 ATTIVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKGFAFQS 548
             TIVGKPIS+ +A+FSSRGPNS SP ILK PDIAAPGSNI+AA+ P +   DKGFA  S
Sbjct: 494 TRTIVGKPISSRIAYFSSRGPNSVSPGILK-PDIAAPGSNIIAAVPPNHKGGDKGFAAMS 553

Query: 549 GTSMAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKLADPFD 608
           GTSMA PHISGIVAL+KSL PTWSPAAI+SAL TTARV+ PSG PI AE SPPK+ADPFD
Sbjct: 554 GTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKVADPFD 613

Query: 609 YGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSVLDL 668
           YGGG+V+ NAA DPGLIYDL   DY+ YY+C MGY  ++IS L++QK  CPS+R+SVLDL
Sbjct: 614 YGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRASVLDL 673

Query: 669 NLPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTVKKISF 728
           NLP+ITVPAL NSTTVTRTVTNVGN ++VY+AVI+AP G+KV V P +L FN  VKKISF
Sbjct: 674 NLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNVKKISF 733

Query: 729 KVTISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTD 763
           KV  S+++ RNYGY FGSL W+DG+H VK PLSVR D
Sbjct: 734 KVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFD 755

BLAST of MS026285 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 767.7 bits (1981), Expect = 8.8e-222
Identity = 402/756 (53.17%), Postives = 516/756 (68.25%), Query Frame = 0

Query: 23  KSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVLGSKEKSWESMVYSYRHGFS 82
           +S        RKVHIVYLGE QH+D +  TE+HH +L ++LGSKE + +SMVYSYRHGFS
Sbjct: 22  RSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFS 81

Query: 83  GFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGE 142
           GFAAKLT SQA+K+A++  VV V+P+S YK+ TTR+WD+LGLS +  +S  LLH   MGE
Sbjct: 82  GFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS--LLHETNMGE 141

Query: 143 DVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGLI 202
            +IIGV+D+G+WPESE F D G GPVPS WKG CE+GE FNS+NCN+K+IGA+++  G +
Sbjct: 142 QIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFL 201

Query: 203 ADGGQQALAN--EYLSARDFQGHGTHVASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAI 262
           A+       N  +++S RD  GHGTHV++ A GSFVPN++Y+GL  GTVRGGAPRA +A+
Sbjct: 202 AENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAM 261

Query: 263 YKVLW-----SEGAGSGADILMAMDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGA 322
           YK  W          S ADIL AMD+A+HDGVDVLS+SL    VP+  E      +  GA
Sbjct: 262 YKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLG-SSVPLYGETDIRDGITTGA 321

Query: 323 FHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSF 382
           FHA  +GI+VVC+GGN GPD  T+ NTAPWI TVAA+T+DR+F  P+TLG+N   LGQ+ 
Sbjct: 322 FHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQAM 381

Query: 383 FAGENDIVGKLVC------STRRRLCSCKDILGN-DTSLSGNIVLCFNKLVDVETTQKAV 442
           + G       LV       S      +C+++L N + ++ G +VLCF           A 
Sbjct: 382 YTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPYGGAVLSAA 441

Query: 443 VAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPMVRIGRATT 502
             V++A G G+I+A      + P   DFPC+ +D ++GT    Y   + +P+V+I  + T
Sbjct: 442 RYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKT 501

Query: 503 IVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKGFAFQSGTS 562
           +VG+P+ T VA FSSRGPNS +PAILK PDIAAPG +ILAA +    FSD+GF   SGTS
Sbjct: 502 LVGQPVGTKVATFSSRGPNSIAPAILK-PDIAAPGVSILAA-TTNTTFSDQGFIMLSGTS 561

Query: 563 MAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKLADPFDYGG 622
           MAAP ISG+ ALLK+LH  WSPAAIRSA+ TTA    P G  I AE SPPKLADPFDYGG
Sbjct: 562 MAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGG 621

Query: 623 GLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSVLDLNLP 682
           GLVNP  +A+PGL+YD+ + DYV  Y+C +GY  + ISQL  + T C + + SVLD NLP
Sbjct: 622 GLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLP 681

Query: 683 SITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTVKKISFKVT 742
           SIT+P L++  T+TRTVTNVG  +SVYR  +  P G +V V P  L FN T KK+ FKV 
Sbjct: 682 SITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVK 741

Query: 743 ISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTDLL 765
           +S +   N GY FGSL WSD LH+V IPLSVRT +L
Sbjct: 742 VSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 771

BLAST of MS026285 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 767.7 bits (1981), Expect = 8.8e-222
Identity = 402/756 (53.17%), Postives = 516/756 (68.25%), Query Frame = 0

Query: 23  KSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVLGSKEKSWESMVYSYRHGFS 82
           +S        RKVHIVYLGE QH+D +  TE+HH +L ++LGSKE + +SMVYSYRHGFS
Sbjct: 38  RSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFS 97

Query: 83  GFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGE 142
           GFAAKLT SQA+K+A++  VV V+P+S YK+ TTR+WD+LGLS +  +S  LLH   MGE
Sbjct: 98  GFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS--LLHETNMGE 157

Query: 143 DVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGLI 202
            +IIGV+D+G+WPESE F D G GPVPS WKG CE+GE FNS+NCN+K+IGA+++  G +
Sbjct: 158 QIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFL 217

Query: 203 ADGGQQALAN--EYLSARDFQGHGTHVASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAI 262
           A+       N  +++S RD  GHGTHV++ A GSFVPN++Y+GL  GTVRGGAPRA +A+
Sbjct: 218 AENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAM 277

Query: 263 YKVLW-----SEGAGSGADILMAMDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGA 322
           YK  W          S ADIL AMD+A+HDGVDVLS+SL    VP+  E      +  GA
Sbjct: 278 YKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLG-SSVPLYGETDIRDGITTGA 337

Query: 323 FHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSF 382
           FHA  +GI+VVC+GGN GPD  T+ NTAPWI TVAA+T+DR+F  P+TLG+N   LGQ+ 
Sbjct: 338 FHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQAM 397

Query: 383 FAGENDIVGKLVC------STRRRLCSCKDILGN-DTSLSGNIVLCFNKLVDVETTQKAV 442
           + G       LV       S      +C+++L N + ++ G +VLCF           A 
Sbjct: 398 YTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPYGGAVLSAA 457

Query: 443 VAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPMVRIGRATT 502
             V++A G G+I+A      + P   DFPC+ +D ++GT    Y   + +P+V+I  + T
Sbjct: 458 RYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKT 517

Query: 503 IVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKGFAFQSGTS 562
           +VG+P+ T VA FSSRGPNS +PAILK PDIAAPG +ILAA +    FSD+GF   SGTS
Sbjct: 518 LVGQPVGTKVATFSSRGPNSIAPAILK-PDIAAPGVSILAA-TTNTTFSDQGFIMLSGTS 577

Query: 563 MAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKLADPFDYGG 622
           MAAP ISG+ ALLK+LH  WSPAAIRSA+ TTA    P G  I AE SPPKLADPFDYGG
Sbjct: 578 MAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGG 637

Query: 623 GLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSVLDLNLP 682
           GLVNP  +A+PGL+YD+ + DYV  Y+C +GY  + ISQL  + T C + + SVLD NLP
Sbjct: 638 GLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLP 697

Query: 683 SITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTVKKISFKVT 742
           SIT+P L++  T+TRTVTNVG  +SVYR  +  P G +V V P  L FN T KK+ FKV 
Sbjct: 698 SITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVK 757

Query: 743 ISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTDLL 765
           +S +   N GY FGSL WSD LH+V IPLSVRT +L
Sbjct: 758 VSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 787

BLAST of MS026285 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 765.4 bits (1975), Expect = 4.4e-221
Identity = 404/745 (54.23%), Postives = 512/745 (68.72%), Query Frame = 0

Query: 34  KVHIVYLGETQHEDSKLTTETHHELLATVLGSKEKSWESMVYSYRHGFSGFAAKLTNSQA 93
           KVHIVYLGE QH+D +  TE+HH +L ++LGSKE +  SMV+SYRHGFSGFAAKLT SQA
Sbjct: 29  KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQA 88

Query: 94  QKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGEDVIIGVLDSGI 153
           +KLA++  VV V P+S Y++ TTR+WD+LGLS   +   NLL+   MGE+VIIG++DSG+
Sbjct: 89  KKLADLPEVVHVTPDSFYQLDTTRTWDYLGLSV--ANPKNLLNDTNMGEEVIIGIVDSGV 148

Query: 154 WPESEAFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGLIA--DGGQQALA 213
           WPESE F D G+GPVPS WKG C SGE F S+ CN+K+IGA+++  G +A  +      +
Sbjct: 149 WPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQCNKKLIGAKYFINGFLATHESFNSTES 208

Query: 214 NEYLSARDFQGHGTHVASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLW-----S 273
            +++S RD  GHGTHVA+ A GS+VP+++Y+GL  GTVRGGAPRA +A+YK  W      
Sbjct: 209 LDFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLDRFD 268

Query: 274 EGAGSGADILMAMDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVV 333
               S ADIL AMD+A+HDGVDVLSLS+ +   P  PE    +V+A GAFHA  +GI+VV
Sbjct: 269 INTCSSADILKAMDEAMHDGVDVLSLSIGY-RFPYFPETDVRAVIATGAFHAVLKGITVV 328

Query: 334 CAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSFFAGENDIVGKL 393
           C+GGN GP  QT+ NTAPWI TVAA+T+DR+F  P+TLG+N   LGQ+ + G       L
Sbjct: 329 CSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILGQAMYTGPELGFTSL 388

Query: 394 VC------STRRRLCSCKDILGNDT-SLSGNIVLCFNKLVDVETTQKAVVAVRQANGNGI 453
           V       S       C+ +  N   +++G +VLCF       T   AV  V++A G G+
Sbjct: 389 VYPENPGNSNESFSGDCELLFFNSNHTMAGKVVLCFTTSTRYITVSSAVSYVKEAGGLGV 448

Query: 454 IVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPMVRIGRATTIVGKPISTNVA 513
           IVA    D L P   DFPC+ +D ++GT    Y+  T  P+V+I  + T+VG+P+ T VA
Sbjct: 449 IVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQPSKTLVGQPVGTKVA 508

Query: 514 FFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVA 573
            FSSRGPNS  PAILK PDIAAPG +ILAA +    F+D+GF F SGTSMAAP ISG+VA
Sbjct: 509 DFSSRGPNSIEPAILK-PDIAAPGVSILAATTTNKTFNDRGFIFLSGTSMAAPTISGVVA 568

Query: 574 LLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADP 633
           LLK+LH  WSPAAIRSA+ TTA    P G  I AE SP KLADPFDYGGGLVNP  AA P
Sbjct: 569 LLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKLADPFDYGGGLVNPEKAAKP 628

Query: 634 GLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNST 693
           GL+YDL + DYV  Y+C +GY  + ISQL  + T C + + SVLD NLPSIT+P L++  
Sbjct: 629 GLVYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSNPKPSVLDFNLPSITIPNLKDEV 688

Query: 694 TVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTVKKISFKVTISASIHRNYGY 753
           T+TRT+TNVG   SVY+ VI  P G +V V P  L FN T K++SFKV +S +   N GY
Sbjct: 689 TLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKVKVSTTHKINTGY 748

Query: 754 LFGSLIWSDGLHHVKIPLSVRTDLL 765
            FGSL WSD LH+V IPLSVRT +L
Sbjct: 749 FFGSLTWSDSLHNVTIPLSVRTQIL 768

BLAST of MS026285 vs. TAIR 10
Match: AT4G10510.1 (Subtilase family protein )

HSP 1 Score: 762.7 bits (1968), Expect = 2.8e-220
Identity = 399/743 (53.70%), Postives = 516/743 (69.45%), Query Frame = 0

Query: 35  VHIVYLGETQHEDSKLTTETHHELLATVLGSKEKSWESMVYSYRHGFSGFAAKLTNSQAQ 94
           VHIVYLGE QH+D +  TE+HH +L ++LGSKE++  SMV+S+RHGFSGFAAKLT SQA+
Sbjct: 22  VHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQAK 81

Query: 95  KLAEMAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGEDVIIGVLDSGIW 154
           K+A++  VV V+P+  YK  TTR+WD+LGL  SP+   NLL++  MGE +IIG++DSG+W
Sbjct: 82  KIADLPEVVHVIPDRFYKPATTRTWDYLGL--SPTNPKNLLNQTNMGEQMIIGIIDSGVW 141

Query: 155 PESEAFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGLIA--DGGQQALAN 214
           PESE F D  +GPVPS WKG CESGE+FNS++CN+K+IGA+++    +A  +    + + 
Sbjct: 142 PESEVFNDNEIGPVPSHWKGGCESGEDFNSSHCNKKLIGAKYFINAFLATHESFNSSESL 201

Query: 215 EYLSARDFQGHGTHVASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLW----SEG 274
           +++S R + GHGTHVA+ A GS+VPN +Y+GL  GTVRGGAPRA +A+YK  W       
Sbjct: 202 DFISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLDIA 261

Query: 275 AGSGADILMAMDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVVCA 334
           A S ADIL AMD+AIHDGVDVLSLSL F   P+ PE      +A GAFHA  +GI+VVCA
Sbjct: 262 ACSSADILKAMDEAIHDGVDVLSLSLGF--EPLYPETDVRDGIATGAFHAVLKGITVVCA 321

Query: 335 GGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQSFFAGENDIVGKLVC 394
            GN GP  QT+ NTAPWI TVAA+T+DR+F+ P+TLG+N   LGQ+ + G       LV 
Sbjct: 322 AGNAGPAAQTVGNTAPWILTVAATTLDRSFVTPMTLGNNKVILGQAIYTGTEVGFTSLVY 381

Query: 395 ------STRRRLCSCKDILGN-DTSLSGNIVLCFNKLVDVETTQKAVVAVRQANGNGIIV 454
                 S      +C+ +L N + +++G +VLCF +     +  +A   V++A G G+I+
Sbjct: 382 PENPGNSNESFSGTCERLLINSNRTMAGKVVLCFTESPYSISVTRAAHYVKRAGGLGVII 441

Query: 455 AGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTSNPMVRIGRATTIVGKPISTNVAFF 514
           AGQ  ++L P   DFPC+ +D ++GT    Y+    +P+V+I  + T++G+P+ T VA F
Sbjct: 442 AGQPGNVLRPCLDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVGTKVASF 501

Query: 515 SSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALL 574
           SSRGPN  S AILK PDIAAPG +ILAA +    F+D+GF F SGTSMA P ISGIVALL
Sbjct: 502 SSRGPNPISAAILK-PDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISGIVALL 561

Query: 575 KSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPGL 634
           K+LHP WSPAAIRSA+ TTA    P G  I AE SP K ADPFDYGGGLVNP  A  PGL
Sbjct: 562 KALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKATKPGL 621

Query: 635 IYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNSTTV 694
           +YDL + DYV  Y+C +GY  + ISQL  + T C   + SVLD NLPSIT+P L+   T+
Sbjct: 622 VYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKEEVTL 681

Query: 695 TRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFNPTVKKISFKVTISASIHRNYGYLF 754
            RT+TNVG   SVYR  +  P GT+V V P  L FN T K++SFKV++S +   N GY F
Sbjct: 682 PRTLTNVGPLESVYRVAVEPPLGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINTGYYF 741

Query: 755 GSLIWSDGLHHVKIPLSVRTDLL 765
           GSL WSD LH+V IPLSVRT LL
Sbjct: 742 GSLTWSDSLHNVTIPLSVRTQLL 758

BLAST of MS026285 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 759.2 bits (1959), Expect = 3.1e-219
Identity = 407/767 (53.06%), Postives = 524/767 (68.32%), Query Frame = 0

Query: 12  SFYALFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLATVLGSKEKSWE 71
           S   + S+    ++A A  ++ KVHIVYLGE QH+D K  TE+HH++L+++LGSK+ + E
Sbjct: 7   SVLVVLSLIIVLNVARASAKS-KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHE 66

Query: 72  SMVYSYRHGFSGFAAKLTNSQAQKLAEMAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQS 131
           SMVYSYRHGFSGFAAKLT SQA+K+A+   V+ V+P+S Y++ TTR WD+LG   S   S
Sbjct: 67  SMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYELATTRIWDYLG--PSADNS 126

Query: 132 SNLLHRGKMGEDVIIGVLDSGIWPESEAFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKI 191
            NL+    MG+  IIGV+D+G+WPESE+F D G+GPVPS WKG CE GE F STNCNRK+
Sbjct: 127 KNLVSDTNMGDQTIIGVIDTGVWPESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKL 186

Query: 192 IGARWYNKGLIADGGQQALAN-EYLSARDFQGHGTHVASTAAGSFVPNVNYQGLGAGTVR 251
           IGA+++  G +A+    A  + +Y+SARDF GHGTHVAS A GSFVPNV+Y+GLG GT+R
Sbjct: 187 IGAKYFINGFLAENQFNATESPDYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLR 246

Query: 252 GGAPRACLAIYKVLW--SEGAG---SGADILMAMDDAIHDGVDVLSLSLRFGGVPISPEF 311
           GGAPRA +A+YK  W  +E  G   S +DI+ A+D+AIHDGVDVLS+SL  G VP++ E 
Sbjct: 247 GGAPRARIAMYKACWYINELDGVTCSFSDIMKAIDEAIHDGVDVLSISLG-GRVPLNSET 306

Query: 312 SEDSVVAIGAFHATARGISVVCAGGNDGPDKQTIVNTAPWIFTVAASTIDRAFLAPVTLG 371
                +A GAFHA A+GI VVCAGGN GP  QT+VNTAPWI TVAA+T+DR+F  P+ LG
Sbjct: 307 DLRDGIATGAFHAVAKGIVVVCAGGNAGPSSQTVVNTAPWILTVAATTLDRSFATPIILG 366

Query: 372 DNTTHLGQSFFAGENDIVGKLV--------CSTRRRLCSCKDILGNDTSLSGNIVLCFNK 431
           +N   LGQ+ + G       LV          T   +C   ++  N T ++G +VLCF  
Sbjct: 367 NNQVILGQAMYIGPELGFTSLVYPEDPGNSIDTFSGVCESLNLNSNRT-MAGKVVLCFTT 426

Query: 432 LVDVETTQKAVVAVRQANGNGIIVAGQHDDILFPYGADFPCIVLDPDVGTKFFHYLLGTS 491
             D      A   V+ A G G+I+A      L P   DFPC+ +D ++GT    Y+  T 
Sbjct: 427 ARDFTVVSTAASIVKAAGGLGLIIARNPGYNLAPCSDDFPCVAIDNELGTDILFYIRYTG 486

Query: 492 NPMVRIGRATTIVGKPISTNVAFFSSRGPNSASPAILKQPDIAAPGSNILAAISPRNPFS 551
           +P+V+I  + T+VG+P+ T VA FSSRGPNS SPAILK PDIAAPG +ILAA SP +  +
Sbjct: 487 SPVVKIQPSRTLVGEPVGTKVATFSSRGPNSISPAILK-PDIAAPGVSILAATSPNDTLN 546

Query: 552 DKGFAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALSTTARVKGPSGAPILAESSP 611
             GF  +SGTSMAAP ISG++ALLKSLHP WSPAA RSA+ TTA    P G  I AESS 
Sbjct: 547 AGGFVMRSGTSMAAPVISGVIALLKSLHPDWSPAAFRSAIVTTAWRTDPFGEQIAAESSS 606

Query: 612 PKLADPFDYGGGLVNPNAAADPGLIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPS 671
            K+ DPFDYGGGLVNP  AA+PGLI D+   DYV  YLC  GY +S IS+L  + T C +
Sbjct: 607 LKVPDPFDYGGGLVNPEKAAEPGLILDMDSQDYV-LYLCSAGYNDSSISRLVGKVTVCSN 666

Query: 672 QRSSVLDLNLPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPYGTKVNVEPSMLAFN 731
            + SVLD+NLPSIT+P L++  T+TRTVTNVG   SVY+ ++  P G +V V P  L FN
Sbjct: 667 PKPSVLDINLPSITIPNLKDEVTLTRTVTNVGPVDSVYKVLVEPPLGIQVVVTPETLVFN 726

Query: 732 PTVKKISFKVTISASIHRNYGYLFGSLIWSDGLHHVKIPLSVRTDLL 765
              K +SF V +S +   N G+ FGSL W+D +H+V IP+SVRT +L
Sbjct: 727 SKTKSVSFTVIVSTTHKINTGFYFGSLTWTDSIHNVVIPVSVRTQIL 766