MS026271 (gene) Bitter gourd (TR) v1

Overview
NameMS026271
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCytochrome P450
Locationscaffold63: 1923011 .. 1923955 (-)
RNA-Seq ExpressionMS026271
SyntenyMS026271
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCGTCGGAAGCCGTTGCCGGAATCTTCGCCCTTCTCCTCTTTCTCTATGCCCTCTTCCGGGCATCTCCAATATCCGGTGCTCACCGGAAGAGACTCCCACCCCAACCCGCCGGCGCTTGGCCGCTGATCGGCCACCTCCATCTACTAAGTTCAACAGAACCGGCCCACATAATGTTCGCCAAAATGGCAGACGCCTACGGACCAATCTTCACGCTAAAATTGGGTATGAACAGAGTCTTGATAGTAAGCAGTTGGGAAGTAGCGAGAGAGTGCTTCACCACCAACGACAGAGTATTTTCCTCCCGTCCAAAAATGGTGGCCTCGAAGCTTCTGGGCTACGACCACGCCATGATGGGGTTAAGCCCCTACGGCCCACACTGGCGCCACATGCGCAAAATCGCCACGCTTCAACTCCTCACCAACCACCGCCTCGAGCAGCTACAACACATCAGAGTATCCGAGGTCCGAAGCTCTATTAAGAAGCTTTATGAGTTGTGTGTCGGTAAAAAATTGTTGTTGGTGGATATGAAGACGTGGTTCGGAGATGTGACGTTGAACGTAATATTTAGAATAGTGGTAGGAAAGAGATTCTCGACGGCCGTGGACGGCGGTGGTGATGAGGAGTTCAGGAAGGCGTTGAGAGATTTCTTTGAATGGTTTGGAGTGTTGGTTCCATCGGATGCGTTTCCATTTCTGAGTTGGTTGGATTTGGGAGGCCATGAAAAGGCCATGAAGAAGACGGGGGAAGTCATGGACCAGGTGTTTGATAAATGGCTCGACAAGCACCGGCAGAAGAGAAATTCCGGTGAAGCTGCGGCGGCGGAGGAGCATAGTTTCATGGACGTCATGCTTTCTTCTGTTAAAGATGGCACAGAAATTTCTAGCTACGATGCTGATACAATCACCAAATCTACATGTTTGGTATGTCTTTTTAATTAA

mRNA sequence

ATGGCCTCGTCGGAAGCCGTTGCCGGAATCTTCGCCCTTCTCCTCTTTCTCTATGCCCTCTTCCGGGCATCTCCAATATCCGGTGCTCACCGGAAGAGACTCCCACCCCAACCCGCCGGCGCTTGGCCGCTGATCGGCCACCTCCATCTACTAAGTTCAACAGAACCGGCCCACATAATGTTCGCCAAAATGGCAGACGCCTACGGACCAATCTTCACGCTAAAATTGGGTATGAACAGAGTCTTGATAGTAAGCAGTTGGGAAGTAGCGAGAGAGTGCTTCACCACCAACGACAGAGTATTTTCCTCCCGTCCAAAAATGGTGGCCTCGAAGCTTCTGGGCTACGACCACGCCATGATGGGGTTAAGCCCCTACGGCCCACACTGGCGCCACATGCGCAAAATCGCCACGCTTCAACTCCTCACCAACCACCGCCTCGAGCAGCTACAACACATCAGAGTATCCGAGGTCCGAAGCTCTATTAAGAAGCTTTATGAGTTGTGTGTCGGTAAAAAATTGTTGTTGGTGGATATGAAGACGTGGTTCGGAGATGTGACGTTGAACGTAATATTTAGAATAGTGGTAGGAAAGAGATTCTCGACGGCCGTGGACGGCGGTGGTGATGAGGAGTTCAGGAAGGCGTTGAGAGATTTCTTTGAATGGTTTGGAGTGTTGGTTCCATCGGATGCGTTTCCATTTCTGAGTTGGTTGGATTTGGGAGGCCATGAAAAGGCCATGAAGAAGACGGGGGAAGTCATGGACCAGGTGTTTGATAAATGGCTCGACAAGCACCGGCAGAAGAGAAATTCCGGTGAAGCTGCGGCGGCGGAGGAGCATAGTTTCATGGACGTCATGCTTTCTTCTGTTAAAGATGGCACAGAAATTTCTAGCTACGATGCTGATACAATCACCAAATCTACATGTTTGGTATGTCTTTTTAATTAA

Coding sequence (CDS)

ATGGCCTCGTCGGAAGCCGTTGCCGGAATCTTCGCCCTTCTCCTCTTTCTCTATGCCCTCTTCCGGGCATCTCCAATATCCGGTGCTCACCGGAAGAGACTCCCACCCCAACCCGCCGGCGCTTGGCCGCTGATCGGCCACCTCCATCTACTAAGTTCAACAGAACCGGCCCACATAATGTTCGCCAAAATGGCAGACGCCTACGGACCAATCTTCACGCTAAAATTGGGTATGAACAGAGTCTTGATAGTAAGCAGTTGGGAAGTAGCGAGAGAGTGCTTCACCACCAACGACAGAGTATTTTCCTCCCGTCCAAAAATGGTGGCCTCGAAGCTTCTGGGCTACGACCACGCCATGATGGGGTTAAGCCCCTACGGCCCACACTGGCGCCACATGCGCAAAATCGCCACGCTTCAACTCCTCACCAACCACCGCCTCGAGCAGCTACAACACATCAGAGTATCCGAGGTCCGAAGCTCTATTAAGAAGCTTTATGAGTTGTGTGTCGGTAAAAAATTGTTGTTGGTGGATATGAAGACGTGGTTCGGAGATGTGACGTTGAACGTAATATTTAGAATAGTGGTAGGAAAGAGATTCTCGACGGCCGTGGACGGCGGTGGTGATGAGGAGTTCAGGAAGGCGTTGAGAGATTTCTTTGAATGGTTTGGAGTGTTGGTTCCATCGGATGCGTTTCCATTTCTGAGTTGGTTGGATTTGGGAGGCCATGAAAAGGCCATGAAGAAGACGGGGGAAGTCATGGACCAGGTGTTTGATAAATGGCTCGACAAGCACCGGCAGAAGAGAAATTCCGGTGAAGCTGCGGCGGCGGAGGAGCATAGTTTCATGGACGTCATGCTTTCTTCTGTTAAAGATGGCACAGAAATTTCTAGCTACGATGCTGATACAATCACCAAATCTACATGTTTGGTATGTCTTTTTAATTAA

Protein sequence

MASSEAVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMFAKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCVGKKLLLVDMKTWFGDVTLNVIFRIVVGKRFSTAVDGGGDEEFRKALRDFFEWFGVLVPSDAFPFLSWLDLGGHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISSYDADTITKSTCLVCLFN
Homology
BLAST of MS026271 vs. NCBI nr
Match: XP_022982223.1 (cytochrome P450 CYP82D47-like [Cucurbita maxima])

HSP 1 Score: 486.1 bits (1250), Expect = 2.3e-133
Identity = 234/312 (75.00%), Postives = 272/312 (87.18%), Query Frame = 0

Query: 2   ASSEAVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMF 61
           +SS AVAGIF LL+FLYALF  S    ++ KRLPP+PAGAWP+IGHLHLLS+TEPAHI  
Sbjct: 10  SSSAAVAGIFTLLIFLYALFNISTKLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITL 69

Query: 62  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 121
           AKMAD YGPIFTLKLGM+R LIVSSWE+ARECFTT DR+F+SRPK+VASKLLGY++AMMG
Sbjct: 70  AKMADVYGPIFTLKLGMHRTLIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMG 129

Query: 122 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELC-VGKKLLLVDMKT 181
           LSPYG HWRH+RK+ATL+L TNHRL+QLQH+RVSEV SSIKKLYELC  GK+  LVDMKT
Sbjct: 130 LSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVESSIKKLYELCGNGKEKALVDMKT 189

Query: 182 WFGDVTLNVIFRIVVGKRFSTAVDGGGDEEFRKALRDFFEWFGVLVPSDAFPFLSWLDLG 241
           WFGD+TLN IFR+VVGKRFSTAV+GGG+EE+RKALR+FFEWFGV VPSD+FPFL WLDLG
Sbjct: 190 WFGDITLNTIFRVVVGKRFSTAVEGGGNEEYRKALREFFEWFGVFVPSDSFPFLKWLDLG 249

Query: 242 GHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISSYDA 301
           GHEKAMKKT EV+D+V DKW+++ R+ R S EA  A EH FMDVMLS VKD  E+SSY+A
Sbjct: 250 GHEKAMKKTAEVLDEVLDKWVEERRKMRASDEAKTA-EHDFMDVMLSGVKDYEELSSYEA 309

Query: 302 DTITKSTCLVCL 313
           +T+ KSTCL  +
Sbjct: 310 NTVIKSTCLALI 320

BLAST of MS026271 vs. NCBI nr
Match: XP_023523452.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 481.9 bits (1239), Expect = 4.3e-132
Identity = 231/312 (74.04%), Postives = 272/312 (87.18%), Query Frame = 0

Query: 2   ASSEAVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMF 61
           +SS AVAGIFALL+FLYALF       ++ KRLPP+PAGAWP+IGHLHLLS+TEPAHI  
Sbjct: 7   SSSAAVAGIFALLIFLYALFNIYKRLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITL 66

Query: 62  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 121
           AKMAD YGPIFTL+LGM++ LIVSSWE+ARECFTT DR+F+SRPK+VASKLLGY++AMMG
Sbjct: 67  AKMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMG 126

Query: 122 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELC-VGKKLLLVDMKT 181
           LSPYG HWRH+RK+ATL+L TNHRL+QLQH+RVSEV S+IKKLY LC  GK+  LVDMKT
Sbjct: 127 LSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVESAIKKLYALCGNGKEKALVDMKT 186

Query: 182 WFGDVTLNVIFRIVVGKRFSTAVDGGGDEEFRKALRDFFEWFGVLVPSDAFPFLSWLDLG 241
           WFGD+TLN IFR+VVGKRFSTAV+GGG++E+RKALR+FFEWFGV VPSD+FPFL WLDLG
Sbjct: 187 WFGDITLNTIFRVVVGKRFSTAVEGGGNDEYRKALREFFEWFGVFVPSDSFPFLKWLDLG 246

Query: 242 GHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISSYDA 301
           GHEKAMKKT EV+D VFDKW+++ R+ R S EA  A EH FMDVMLS+VKD  E+SSYDA
Sbjct: 247 GHEKAMKKTAEVLDDVFDKWVEERRKMRASDEAKTA-EHDFMDVMLSTVKDYDELSSYDA 306

Query: 302 DTITKSTCLVCL 313
           +T+ KSTCL  +
Sbjct: 307 NTVIKSTCLALI 317

BLAST of MS026271 vs. NCBI nr
Match: XP_022940489.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 481.5 bits (1238), Expect = 5.6e-132
Identity = 231/312 (74.04%), Postives = 272/312 (87.18%), Query Frame = 0

Query: 2   ASSEAVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMF 61
           +SS AVAGIFALL+FLY LF  S    ++ KRLPP+PAGAWP+IGHLHLLS+TEPAHI  
Sbjct: 7   SSSAAVAGIFALLIFLYVLFNISKRLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITL 66

Query: 62  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 121
           AKMAD YGPIFTL+LGM++ LIVSSWE+ARECFTT DR+F+SRPK+VASKLLGY++AMMG
Sbjct: 67  AKMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMG 126

Query: 122 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELC-VGKKLLLVDMKT 181
           LSPYG HWRH+RK+ATL+L TNHRL+QLQH+RVSEV SSIKKLYELC  GK+  LVDM T
Sbjct: 127 LSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVDSSIKKLYELCGNGKEKALVDMTT 186

Query: 182 WFGDVTLNVIFRIVVGKRFSTAVDGGGDEEFRKALRDFFEWFGVLVPSDAFPFLSWLDLG 241
           WFGD+TLN IFR+VVGKRFSTAV+GGG++E+RKALR+FFEWFGV VPSD+FPFL WLDLG
Sbjct: 187 WFGDITLNTIFRVVVGKRFSTAVEGGGNDEYRKALREFFEWFGVFVPSDSFPFLKWLDLG 246

Query: 242 GHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISSYDA 301
           GHEKAMKKT EV+D+VFDKW+++ R+ R S EA  A EH FMDVMLS+VKD  E+SSYDA
Sbjct: 247 GHEKAMKKTAEVLDEVFDKWVEERRKMRASDEAKTA-EHDFMDVMLSTVKDCDELSSYDA 306

Query: 302 DTITKSTCLVCL 313
           + + KSTCL  +
Sbjct: 307 NIVIKSTCLALI 317

BLAST of MS026271 vs. NCBI nr
Match: XP_023535456.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 466.5 bits (1199), Expect = 1.9e-127
Identity = 225/318 (70.75%), Postives = 266/318 (83.65%), Query Frame = 0

Query: 2   ASSEAVAGIFALLLFLYALFRA-SPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIM 61
           +SS+  AGIFAL++FLYAL +      GAHRKRLPP+  GA PLIGHLH L+  EPAHI 
Sbjct: 7   SSSDVFAGIFALIIFLYALSKIYGGTFGAHRKRLPPEAGGALPLIGHLHRLAGNEPAHIA 66

Query: 62  FAKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMM 121
           FAKMADAYGPIFT++LG+   LIVS+WE+ARECF+TNDR+F+SRPK+VASKLLGYD+AMM
Sbjct: 67  FAKMADAYGPIFTVRLGVYTNLIVSNWEIARECFSTNDRIFASRPKLVASKLLGYDYAMM 126

Query: 122 GLSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCV------GKKLL 181
           GLSPYGPHWRH+RK+ATL+LLTN RLEQLQHIRV EVRSSIKKLYE+CV        + +
Sbjct: 127 GLSPYGPHWRHVRKLATLELLTNQRLEQLQHIRVFEVRSSIKKLYEVCVKGRSNGSNEGV 186

Query: 182 LVDMKTWFGDVTLNVIFRIVVGKRFSTAVDGGGDEEFRKALRDFFEWFGVLVPSDAFPFL 241
           LV+MKTWFGD+TLN IFRIVVGKRFSTAV+G G+EE+R ALRDFFEWFGV VPSD+FPFL
Sbjct: 187 LVEMKTWFGDITLNTIFRIVVGKRFSTAVEGSGNEEYRTALRDFFEWFGVFVPSDSFPFL 246

Query: 242 SWLDLGGHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTE 301
            WLDLGGHEKAMK T +V+D+VFDKWL +HRQ +N  E    E H FMDVMLS+VKD  +
Sbjct: 247 KWLDLGGHEKAMKNTAKVLDEVFDKWLQEHRQNKNVSEGKMEEPHHFMDVMLSNVKDDGQ 306

Query: 302 ISSYDADTITKSTCLVCL 313
           +S+YDA T+TK+TCL  +
Sbjct: 307 LSNYDAHTVTKATCLALI 324

BLAST of MS026271 vs. NCBI nr
Match: XP_038897198.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 459.5 bits (1181), Expect = 2.3e-125
Identity = 225/319 (70.53%), Postives = 266/319 (83.39%), Query Frame = 0

Query: 5   EAVAGIFALLLFLYALFRASPISGA----HRKRLPPQPAGAWPLIGHLHLLSSTEPAHIM 64
           + VAGIFA+++FLYAL +   I G     HRKRLPP+ +GA PLIGHLHLL + EPAH  
Sbjct: 10  DTVAGIFAVIIFLYALLK---IYGGRVRPHRKRLPPEASGALPLIGHLHLLDANEPAHTT 69

Query: 65  FAKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMM 124
           FAKMADAYGPIFTL+LG    LIVS+W++ARECFTTNDR+F+SRPK+VASKLLGYD+AM+
Sbjct: 70  FAKMADAYGPIFTLRLGAYTNLIVSNWKIARECFTTNDRIFASRPKLVASKLLGYDYAML 129

Query: 125 GLSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCV-----GKKLLL 184
           GLSPYGPHWRH+RKIA L+LLTNHRLE+LQHIRVSEV++S+KKLYELCV      +K  L
Sbjct: 130 GLSPYGPHWRHVRKIAMLELLTNHRLEKLQHIRVSEVQTSMKKLYELCVRNKDNSEKKTL 189

Query: 185 VDMKTWFGDVTLNVIFRIVVGKRFSTAVDGGG--DEEFRKALRDFFEWFGVLVPSDAFPF 244
           V+MKTWFGD+TLN IFRIVVGKRFSTAVD  G  +EE+RKALRDFFEWFGV VPSD+FPF
Sbjct: 190 VEMKTWFGDITLNTIFRIVVGKRFSTAVDASGNDNEEYRKALRDFFEWFGVFVPSDSFPF 249

Query: 245 LSWLDLGGHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGT 304
           L WLDLGGHEKAMKKT +++D+VFDKWL +H+Q RN+      EEH FMDVMLS VKD  
Sbjct: 250 LKWLDLGGHEKAMKKTAKMLDEVFDKWLQEHQQNRNNIGEENVEEHDFMDVMLSRVKDDG 309

Query: 305 EISSYDADTITKSTCLVCL 313
           ++S YDADT+TK+TCL  +
Sbjct: 310 QLSKYDADTVTKATCLALI 325

BLAST of MS026271 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.2e-71
Identity = 136/312 (43.59%), Postives = 206/312 (66.03%), Query Frame = 0

Query: 6   AVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMFAKMA 65
           A+A I +L+     L+R +      R +  P  +GAWP++GHL LL+ ++  H +   +A
Sbjct: 14  AIASILSLIFLCLFLYRKN-----SRGKDAPVVSGAWPILGHLSLLNGSQTPHKVLGALA 73

Query: 66  DAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPY 125
           D YGP+FT+KLGM   L++S+WE+++E FTTND   SSRPK+VA +++ Y+ A +GL+PY
Sbjct: 74  DKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAPY 133

Query: 126 GPHWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCVG-----KKLLLVDMKT 185
           GP+WR +RKI T + L+N R+EQ  HIRVSEVR+SIK+L+++         +  LVD+K 
Sbjct: 134 GPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIKQ 193

Query: 186 WFGDVTLNVIFRIVVGKRFSTAVDGGGDEE---FRKALRDFFEWFGVLVPSDAFPFLSWL 245
           W   +T N++ R+VVGKR+   +   G ++   F K +R+F    G    +D  P L WL
Sbjct: 194 WLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCLRWL 253

Query: 246 DLGGHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISS 305
           DLGGHEKAMK   + +D++  +WL++HRQK+  GE   + +  FMDVM+S++ +G +I +
Sbjct: 254 DLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVES-DRDFMDVMISAL-NGAQIGA 313

Query: 306 YDADTITKSTCL 310
           +DADTI K+T L
Sbjct: 314 FDADTICKATSL 318

BLAST of MS026271 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-70
Identity = 140/312 (44.87%), Postives = 203/312 (65.06%), Query Frame = 0

Query: 9   GIFALL-LFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMFAKMADA 68
           G F LL  F Y +++       ++ + PPQPAGAWP+IGHLHLL+  +  H + +  AD 
Sbjct: 13  GFFVLLPCFFYFVWK----KPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSFADK 72

Query: 69  YGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGP 128
            GP+F ++LG+++ L+V++ E+A+ECFTTNDR F +RP  VA+K++GY++ M+G++PYGP
Sbjct: 73  NGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAPYGP 132

Query: 129 HWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCVGKKL--LLVDMKTWFGDV 188
           +WR MRKI  L+ L+N RL+ L+H+  SE+  S K+LY+L   + +   LVDMK W  D+
Sbjct: 133 YWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDFCLVDMKQWLADL 192

Query: 189 TLNVIFRIVVGKRFSTAVDGGGDEEFR-----KALRDFFEWFGVLVPSDAFPFLSWLDLG 248
           TLN+  ++VVGKRF  +      EE       K LR+ F   G  V SD  P+L WLDLG
Sbjct: 193 TLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYLRWLDLG 252

Query: 249 GHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISSYDA 308
           GHEK MK+T + +D +F  WLD+H++KR SG     ++  FMDVMLS +++       D 
Sbjct: 253 GHEKEMKRTVKELDILFKGWLDEHKRKRLSG-GKEDDDQDFMDVMLSILEESK--LGNDV 312

Query: 309 DTITKSTCLVCL 313
           DTI K+ CL  +
Sbjct: 313 DTINKTACLAII 317

BLAST of MS026271 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 4.1e-69
Identity = 137/306 (44.77%), Postives = 199/306 (65.03%), Query Frame = 0

Query: 14  LLFLYALFRASPISGAH-RKRLPPQPAGAWPLIGHLHLLSSTEPAHIMFAKMADAYGPIF 73
           L+FL  LF   P+   H   +  P   GAWP+ GHL LL  ++  H     +A+ +GP+F
Sbjct: 18  LIFLLCLFLYGPLKKVHGSSKEAPTVGGAWPIFGHLPLLIGSKSPHKALGALAEKHGPLF 77

Query: 74  TLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRHM 133
           T+KLG  + L+VS WE+ARECFTTND   S+RPK++ ++L+ Y++AM+ ++PYGP+WR +
Sbjct: 78  TIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLVAPYGPYWREL 137

Query: 134 RKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCVGKK----LLLVDMKTWFGDVTLN 193
           RKI   ++L++ R+EQLQ +RVSEV++SI +LY++   +K       V++K WF     N
Sbjct: 138 RKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVELKQWFAQPIFN 197

Query: 194 VIFRIVVGKRF--STAVDGGGDEEFRKALRDFFEWFGVLVPSDAFPFLSWLDLGGHEKAM 253
           ++ R+VVGKRF  +TA D    E+  KA+ +F    GV    DA P+L WLD GG+EKAM
Sbjct: 198 MVLRMVVGKRFLSATATDEKA-EKCVKAVDEFMRLAGVFTVGDAIPYLRWLDFGGYEKAM 257

Query: 254 KKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISSYDADTITKS 313
           K+T + +D +  +WL++HRQKR  GE     +  FM+VMLSS+ DG  I   DADT+ KS
Sbjct: 258 KETAKELDVMISEWLEEHRQKRALGEGVDGAQ-DFMNVMLSSL-DGKTIDGIDADTLIKS 317

BLAST of MS026271 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 261.5 bits (667), Expect = 1.2e-68
Identity = 145/325 (44.62%), Postives = 196/325 (60.31%), Query Frame = 0

Query: 12  ALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMFAKMADAYGPI 71
           +L+  L+ LFR S +S  H K  PP  +G+WPL+GHL L+ +T+  H     + D YGPI
Sbjct: 15  SLISLLFFLFRFSKVS--HTKE-PPIISGSWPLLGHLPLMRNTQTPHKTLGALVDKYGPI 74

Query: 72  FTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRH 131
           FT+KLG    L++S+WE+A+ECFT ND V SSRPK VA +L+ Y+ A +G +PYG +WR 
Sbjct: 75  FTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPYGAYWRQ 134

Query: 132 MRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKL-----------YELCVGKK-------- 191
           +RKI TL++L+N R+E L HIRVSEV++SIK+L           Y L    K        
Sbjct: 135 LRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKSSSTNDEP 194

Query: 192 ----LLLVDMKTWFGDVTLNVIFRIVVGKRFSTAVDGGGDEE---FRKALRDFFEWFGVL 251
                + V++K WF  +TLN++ R+VVGKR    VD    EE   F + +RDF    G  
Sbjct: 195 STTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENIRDFMRLIGTF 254

Query: 252 VPSDAFPFLSWLDLGGHEKAMKKTGEVMDQVFDKWLDKHRQKRNSG-EAAAAEEHSFMDV 310
              D  PFL WLDLGGHEK MKK  +  D + ++WL++HR+K+  G E     E  FMD 
Sbjct: 255 TVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDKVVGERDFMDA 314

BLAST of MS026271 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.8e-67
Identity = 132/291 (45.36%), Postives = 183/291 (62.89%), Query Frame = 0

Query: 36  PQPAGAWPLIGHLHLLSSTEP-AHIMFAKMADAYGPIFTLKLGMNRVLIVSSWEVARECF 95
           P P+GAWP+IGHLHLL   E   +    KMAD YGP  +L+LG N   +VSS+EVA++CF
Sbjct: 33  PAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCF 92

Query: 96  TTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRV 155
           T ND+  +SRP   A+K +GY+ A+ G +PY   WR MRKIAT++LL+N RL+ L+H+RV
Sbjct: 93  TVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRV 152

Query: 156 SEVRSSIKKLYELCV---GKKLLLVDMKTWFGDVTLNVIFRIVVGKRFSTAVDGGGD--- 215
           SE+   +K LY L     G K ++VD+K+W  D+TLN+I R+V GKR+     GGG    
Sbjct: 153 SEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIVRMVAGKRY---FGGGGSVSS 212

Query: 216 ------EEFRKALRDFFEWFGVLVPSDAFPFLSWLDLGGHEKAMKKTGEVMDQVFDKWLD 275
                  + +KA+  FF   G+   SDAFP LS+ DL GHEK MK+TG  +D + ++W++
Sbjct: 213 EDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIE 272

Query: 276 KHRQKRNSGEAAAAEEHSFMDVMLSSVKDG-TEISSYDADTITKSTCLVCL 313
            HRQ+R         +  F+DVM+S  + G      YDA+T  KSTCL  +
Sbjct: 273 NHRQQRKF-SGTKENDSDFIDVMMSLAEQGKLSHLQYDANTSIKSTCLALI 319

BLAST of MS026271 vs. ExPASy TrEMBL
Match: A0A6J1J4B0 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111481116 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.1e-133
Identity = 234/312 (75.00%), Postives = 272/312 (87.18%), Query Frame = 0

Query: 2   ASSEAVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMF 61
           +SS AVAGIF LL+FLYALF  S    ++ KRLPP+PAGAWP+IGHLHLLS+TEPAHI  
Sbjct: 10  SSSAAVAGIFTLLIFLYALFNISTKLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITL 69

Query: 62  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 121
           AKMAD YGPIFTLKLGM+R LIVSSWE+ARECFTT DR+F+SRPK+VASKLLGY++AMMG
Sbjct: 70  AKMADVYGPIFTLKLGMHRTLIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMG 129

Query: 122 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELC-VGKKLLLVDMKT 181
           LSPYG HWRH+RK+ATL+L TNHRL+QLQH+RVSEV SSIKKLYELC  GK+  LVDMKT
Sbjct: 130 LSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVESSIKKLYELCGNGKEKALVDMKT 189

Query: 182 WFGDVTLNVIFRIVVGKRFSTAVDGGGDEEFRKALRDFFEWFGVLVPSDAFPFLSWLDLG 241
           WFGD+TLN IFR+VVGKRFSTAV+GGG+EE+RKALR+FFEWFGV VPSD+FPFL WLDLG
Sbjct: 190 WFGDITLNTIFRVVVGKRFSTAVEGGGNEEYRKALREFFEWFGVFVPSDSFPFLKWLDLG 249

Query: 242 GHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISSYDA 301
           GHEKAMKKT EV+D+V DKW+++ R+ R S EA  A EH FMDVMLS VKD  E+SSY+A
Sbjct: 250 GHEKAMKKTAEVLDEVLDKWVEERRKMRASDEAKTA-EHDFMDVMLSGVKDYEELSSYEA 309

Query: 302 DTITKSTCLVCL 313
           +T+ KSTCL  +
Sbjct: 310 NTVIKSTCLALI 320

BLAST of MS026271 vs. ExPASy TrEMBL
Match: A0A6J1FKD2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111446070 PE=3 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.7e-132
Identity = 231/312 (74.04%), Postives = 272/312 (87.18%), Query Frame = 0

Query: 2   ASSEAVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMF 61
           +SS AVAGIFALL+FLY LF  S    ++ KRLPP+PAGAWP+IGHLHLLS+TEPAHI  
Sbjct: 7   SSSAAVAGIFALLIFLYVLFNISKRLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITL 66

Query: 62  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 121
           AKMAD YGPIFTL+LGM++ LIVSSWE+ARECFTT DR+F+SRPK+VASKLLGY++AMMG
Sbjct: 67  AKMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMG 126

Query: 122 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELC-VGKKLLLVDMKT 181
           LSPYG HWRH+RK+ATL+L TNHRL+QLQH+RVSEV SSIKKLYELC  GK+  LVDM T
Sbjct: 127 LSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVDSSIKKLYELCGNGKEKALVDMTT 186

Query: 182 WFGDVTLNVIFRIVVGKRFSTAVDGGGDEEFRKALRDFFEWFGVLVPSDAFPFLSWLDLG 241
           WFGD+TLN IFR+VVGKRFSTAV+GGG++E+RKALR+FFEWFGV VPSD+FPFL WLDLG
Sbjct: 187 WFGDITLNTIFRVVVGKRFSTAVEGGGNDEYRKALREFFEWFGVFVPSDSFPFLKWLDLG 246

Query: 242 GHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISSYDA 301
           GHEKAMKKT EV+D+VFDKW+++ R+ R S EA  A EH FMDVMLS+VKD  E+SSYDA
Sbjct: 247 GHEKAMKKTAEVLDEVFDKWVEERRKMRASDEAKTA-EHDFMDVMLSTVKDCDELSSYDA 306

Query: 302 DTITKSTCLVCL 313
           + + KSTCL  +
Sbjct: 307 NIVIKSTCLALI 317

BLAST of MS026271 vs. ExPASy TrEMBL
Match: A0A6J1F697 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442562 PE=3 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 7.9e-124
Identity = 221/319 (69.28%), Postives = 264/319 (82.76%), Query Frame = 0

Query: 2   ASSEAVAGIFALLLFLYALFRA-SPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIM 61
           +SS+  AGI AL++FLY+L +      GAHR RLPP+  GA PLIGHLH L+  EPAHI 
Sbjct: 7   SSSDVFAGILALIIFLYSLSKIYGGTFGAHRMRLPPEAGGALPLIGHLHRLAGNEPAHIS 66

Query: 62  FAKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMM 121
           FAKMADAYGPIFT++LG+   LIVS+WE+ARECF+T DR+F+SRPK+VASKLLGYD+AMM
Sbjct: 67  FAKMADAYGPIFTVRLGVYTNLIVSNWEIARECFSTKDRIFASRPKLVASKLLGYDYAMM 126

Query: 122 GLSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCV------GKKLL 181
           GLSPYGPHWRH+RK+ATL+LLTN RLEQLQHIRV EVRSSIKKLYE+CV        + +
Sbjct: 127 GLSPYGPHWRHVRKLATLELLTNQRLEQLQHIRVFEVRSSIKKLYEVCVKGRNNGSNEGV 186

Query: 182 LVDMKTWFGDVTLNVIFRIVVGKRFSTAVDGGGDEEFRKALRDFFEWFGVLVPSDAFPFL 241
           LV+MKTWFGD+TLN IFRIVVGKRFSTAV+G G+EE+R ALRDFFEWFGV VPSD+FPFL
Sbjct: 187 LVEMKTWFGDITLNTIFRIVVGKRFSTAVEGSGNEEYRTALRDFFEWFGVFVPSDSFPFL 246

Query: 242 SWLDLGGHEKAMKKTGEVMDQVFDKWLDKHRQ-KRNSGEAAAAEEHSFMDVMLSSVKDGT 301
            WLDLGGHEKAMK T +V+D+VFDKWL +HRQ K+N GE    +   FMDVMLS+VKD  
Sbjct: 247 KWLDLGGHEKAMKNTAKVLDEVFDKWLQEHRQKKKNLGEGKMEDPPDFMDVMLSNVKDDG 306

Query: 302 EISSYDADTITKSTCLVCL 313
           ++S+YDA T+TK+TCL  +
Sbjct: 307 QLSNYDAHTVTKATCLALI 325

BLAST of MS026271 vs. ExPASy TrEMBL
Match: A0A5D3BG48 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00680 PE=3 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 4.3e-122
Identity = 211/310 (68.06%), Postives = 258/310 (83.23%), Query Frame = 0

Query: 3   SSEAVAGIFALLLFLYALFRASP-ISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMF 62
           S+  V GIF+LLLFLY LF  S  + GAHRK+LPP+PAG WP+IGHLHLLS+TEPAHI  
Sbjct: 9   SATVVTGIFSLLLFLYTLFNISTRVVGAHRKKLPPEPAGHWPVIGHLHLLSATEPAHITL 68

Query: 63  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 122
           AK+AD YGPIFTLKLGM++ L+VSSWE+A+ECFTTNDR+F+SRPK+VASKLLGY++ M+G
Sbjct: 69  AKLADTYGPIFTLKLGMSKALVVSSWEIAKECFTTNDRIFASRPKLVASKLLGYNYNMLG 128

Query: 123 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCVGKKLLLVDMKTW 182
           LSPYGP+WRH+RK+ATL+LLTN RL Q QHIR  EV++SIKKLYE C+  K  LV+M TW
Sbjct: 129 LSPYGPYWRHIRKLATLELLTNRRLHQFQHIREFEVQTSIKKLYESCIRNKKALVEMNTW 188

Query: 183 FGDVTLNVIFRIVVGKRFSTAVDGGGD--EEFRKALRDFFEWFGVLVPSDAFPFLSWLDL 242
           FGD+TLN IFRIVVGK+FS A+DG  D  E +R ALR+FFEWFGV VPSD+FPFL W DL
Sbjct: 189 FGDITLNTIFRIVVGKKFSIAMDGSADRNEVYRTALREFFEWFGVFVPSDSFPFLKWFDL 248

Query: 243 GGHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISSYD 302
           GGHEKAMKKT +++D+VFDKWL +HRQ+R   E+    E+ FMDV+LS+VKD  +  +YD
Sbjct: 249 GGHEKAMKKTAKILDEVFDKWLQEHRQRRKFEES----ENDFMDVLLSNVKDAEQFPNYD 308

Query: 303 ADTITKSTCL 310
           ADT+ KS+CL
Sbjct: 309 ADTVIKSSCL 314

BLAST of MS026271 vs. ExPASy TrEMBL
Match: A0A1S3CMB9 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502574 PE=3 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 4.3e-122
Identity = 211/310 (68.06%), Postives = 258/310 (83.23%), Query Frame = 0

Query: 3   SSEAVAGIFALLLFLYALFRASP-ISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMF 62
           S+  V GIF+LLLFLY LF  S  + GAHRK+LPP+PAG WP+IGHLHLLS+TEPAHI  
Sbjct: 9   SATVVTGIFSLLLFLYTLFNISTRVVGAHRKKLPPEPAGHWPVIGHLHLLSATEPAHITL 68

Query: 63  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 122
           AK+AD YGPIFTLKLGM++ L+VSSWE+A+ECFTTNDR+F+SRPK+VASKLLGY++ M+G
Sbjct: 69  AKLADTYGPIFTLKLGMSKALVVSSWEIAKECFTTNDRIFASRPKLVASKLLGYNYNMLG 128

Query: 123 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCVGKKLLLVDMKTW 182
           LSPYGP+WRH+RK+ATL+LLTN RL Q QHIR  EV++SIKKLYE C+  K  LV+M TW
Sbjct: 129 LSPYGPYWRHIRKLATLELLTNRRLHQFQHIREFEVQTSIKKLYESCIRNKKALVEMNTW 188

Query: 183 FGDVTLNVIFRIVVGKRFSTAVDGGGD--EEFRKALRDFFEWFGVLVPSDAFPFLSWLDL 242
           FGD+TLN IFRIVVGK+FS A+DG  D  E +R ALR+FFEWFGV VPSD+FPFL W DL
Sbjct: 189 FGDITLNTIFRIVVGKKFSIAMDGSADRNEVYRTALREFFEWFGVFVPSDSFPFLKWFDL 248

Query: 243 GGHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISSYD 302
           GGHEKAMKKT +++D+VFDKWL +HRQ+R   E+    E+ FMDV+LS+VKD  +  +YD
Sbjct: 249 GGHEKAMKKTAKILDEVFDKWLQEHRQRRKFEES----ENDFMDVLLSNVKDAEQFPNYD 308

Query: 303 ADTITKSTCL 310
           ADT+ KS+CL
Sbjct: 309 ADTVIKSSCL 314

BLAST of MS026271 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 256.5 bits (654), Expect = 2.7e-68
Identity = 132/291 (45.36%), Postives = 183/291 (62.89%), Query Frame = 0

Query: 36  PQPAGAWPLIGHLHLLSSTEP-AHIMFAKMADAYGPIFTLKLGMNRVLIVSSWEVARECF 95
           P P+GAWP+IGHLHLL   E   +    KMAD YGP  +L+LG N   +VSS+EVA++CF
Sbjct: 33  PAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCF 92

Query: 96  TTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRHMRKIATLQLLTNHRLEQLQHIRV 155
           T ND+  +SRP   A+K +GY+ A+ G +PY   WR MRKIAT++LL+N RL+ L+H+RV
Sbjct: 93  TVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRV 152

Query: 156 SEVRSSIKKLYELCV---GKKLLLVDMKTWFGDVTLNVIFRIVVGKRFSTAVDGGGD--- 215
           SE+   +K LY L     G K ++VD+K+W  D+TLN+I R+V GKR+     GGG    
Sbjct: 153 SEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIVRMVAGKRY---FGGGGSVSS 212

Query: 216 ------EEFRKALRDFFEWFGVLVPSDAFPFLSWLDLGGHEKAMKKTGEVMDQVFDKWLD 275
                  + +KA+  FF   G+   SDAFP LS+ DL GHEK MK+TG  +D + ++W++
Sbjct: 213 EDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIE 272

Query: 276 KHRQKRNSGEAAAAEEHSFMDVMLSSVKDG-TEISSYDADTITKSTCLVCL 313
            HRQ+R         +  F+DVM+S  + G      YDA+T  KSTCL  +
Sbjct: 273 NHRQQRKF-SGTKENDSDFIDVMMSLAEQGKLSHLQYDANTSIKSTCLALI 319

BLAST of MS026271 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 251.1 bits (640), Expect = 1.1e-66
Identity = 138/315 (43.81%), Postives = 195/315 (61.90%), Query Frame = 0

Query: 13  LLLFLY-ALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEP-AHIMFAKMADAYGP 72
           +L+F++ ALF+ S     H K   P P+GAWP+IGHLHLLS  E   +    KMAD YGP
Sbjct: 12  ILVFVFIALFKKSK-KPKHVK--APAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGP 71

Query: 73  IFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWR 132
             +L+LG +   +VSS+EVA++CFT ND+  +SRP   A+K +GYD A+ G +PY   WR
Sbjct: 72  AMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWR 131

Query: 133 HMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCV---GKKLLLVDMKTWFGDVTL 192
            MRKIATL+LL+N RL+ L+H+RVSE+   ++ LY L V   G + ++VD+K+W  D++L
Sbjct: 132 EMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDLKSWLEDMSL 191

Query: 193 NVIFRIVVGKRFSTAVDGGGD---------EEFRKALRDFFEWFGVLVPSDAFPFLSWLD 252
           N++ R+V GKR+     GGG           + RK + +FF   G+   SDAFP L W D
Sbjct: 192 NMMVRMVAGKRYF----GGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFD 251

Query: 253 LGGHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGT-EISS 312
             GHEK MK+TG  +D + ++W++ HRQ+R         +  F+DVMLS  + G      
Sbjct: 252 FQGHEKEMKQTGRELDVILERWIENHRQQRKV-SGTKHNDSDFVDVMLSLAEQGKFSHLQ 311

BLAST of MS026271 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 213.4 bits (542), Expect = 2.6e-55
Identity = 126/316 (39.87%), Postives = 182/316 (57.59%), Query Frame = 0

Query: 12  ALLLFLY-ALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEP-AHIMFAKMADAYG 71
           ++L+F++ ALF+ S      +    P P+GAWP+IGHLHLL   E   +    KMAD YG
Sbjct: 11  SILVFVFIALFKKSK---KPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYG 70

Query: 72  PIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHW 131
           P  +L+LG +   + SS+EVA++CFT ND+  +S     A+K +GY             W
Sbjct: 71  PAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGY-----------VFW 130

Query: 132 RHMRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCV---GKKLLLVDMKTWFGDVT 191
             MRKIA ++LL+N RL+ L ++RVSE+   +K LY L V   G + ++VD+K+W  D+ 
Sbjct: 131 LEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVMVDLKSWLEDMI 190

Query: 192 LNVIFRIVVGKRFSTAVDGGGDE---------EFRKALRDFFEWFGVLVPSDAFPFLSWL 251
            N+I R+V GKR+     GGG E         ++RK +  FF   G+   SDAFP L WL
Sbjct: 191 ANMIMRMVAGKRY---FGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWL 250

Query: 252 DLGGHEKAMKKTGEVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDG-TEIS 311
           DL GHEK MK+T   +D + ++W++ HRQ+R         +  F+DVMLS  + G     
Sbjct: 251 DLQGHEKEMKQTRRELDVILERWIENHRQQRKV-SGTKHNDSDFVDVMLSLAEQGKLSHL 307

Query: 312 SYDADTITKSTCLVCL 313
            YDA+T  K+TCL  +
Sbjct: 311 QYDANTCIKTTCLALI 307

BLAST of MS026271 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 187.6 bits (475), Expect = 1.6e-47
Identity = 109/299 (36.45%), Postives = 169/299 (56.52%), Query Frame = 0

Query: 12  ALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMFAKMADAYGPI 71
           AL++F Y   R             P+P GA PL GHLHLL   +      A M+  +GPI
Sbjct: 16  ALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGPI 75

Query: 72  FTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRH 131
           F+LKLG  R+++ S  +  ++CFTTND   ++RP +   + +GY++A + L+PYG +WR 
Sbjct: 76  FSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRE 135

Query: 132 MRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCVGKKLLLVDMKTWFGDVTLNVIF 191
           +RKI T+ L +NH +E L HIR SEV + IK LY+   G  ++ +DM   F  +T N+I 
Sbjct: 136 LRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYKGNGGTSIVKIDM--LFEFLTFNIIL 195

Query: 192 RIVVGKRFSTAVDGGGDEEFRKALRDFFEWFGVL-VPSDAFPFLSWLDLGGHEKAMKKTG 251
           R +VGKR         +  +++AL+   E+  V+ +  D  P+L WLD   + + MK+  
Sbjct: 196 RKMVGKRIGFGEVNSDEWRYKEALK-HCEYLAVIPMIGDVIPWLGWLDFAKNSQ-MKRLF 255

Query: 252 EVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISSYDADTITKSTCL 310
           + +D V  KWL +H +KR+  E    +E + MD++L  + +   IS +  D I K+T L
Sbjct: 256 KELDSVNTKWLHEHLKKRSRNE--KDQERTIMDLLLDILPEDIVISGHVRDVIVKATIL 308

BLAST of MS026271 vs. TAIR 10
Match: AT3G25180.2 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 187.6 bits (475), Expect = 1.6e-47
Identity = 109/299 (36.45%), Postives = 169/299 (56.52%), Query Frame = 0

Query: 12  ALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIMFAKMADAYGPI 71
           AL++F Y   R             P+P GA PL GHLHLL   +      A M+  +GPI
Sbjct: 16  ALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGPI 75

Query: 72  FTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRH 131
           F+LKLG  R+++ S  +  ++CFTTND   ++RP +   + +GY++A + L+PYG +WR 
Sbjct: 76  FSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRE 135

Query: 132 MRKIATLQLLTNHRLEQLQHIRVSEVRSSIKKLYELCVGKKLLLVDMKTWFGDVTLNVIF 191
           +RKI T+ L +NH +E L HIR SEV + IK LY+   G  ++ +DM   F  +T N+I 
Sbjct: 136 LRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYKGNGGTSIVKIDM--LFEFLTFNIIL 195

Query: 192 RIVVGKRFSTAVDGGGDEEFRKALRDFFEWFGVL-VPSDAFPFLSWLDLGGHEKAMKKTG 251
           R +VGKR         +  +++AL+   E+  V+ +  D  P+L WLD   + + MK+  
Sbjct: 196 RKMVGKRIGFGEVNSDEWRYKEALK-HCEYLAVIPMIGDVIPWLGWLDFAKNSQ-MKRLF 255

Query: 252 EVMDQVFDKWLDKHRQKRNSGEAAAAEEHSFMDVMLSSVKDGTEISSYDADTITKSTCL 310
           + +D V  KWL +H +KR+  E    +E + MD++L  + +   IS +  D I K+T L
Sbjct: 256 KELDSVNTKWLHEHLKKRSRNE--KDQERTIMDLLLDILPEDIVISGHVRDVIVKATIL 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022982223.12.3e-13375.00cytochrome P450 CYP82D47-like [Cucurbita maxima][more]
XP_023523452.14.3e-13274.04cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo][more]
XP_022940489.15.6e-13274.04cytochrome P450 CYP82D47-like [Cucurbita moschata][more]
XP_023535456.11.9e-12770.75cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo][more]
XP_038897198.12.3e-12570.53cytochrome P450 CYP82D47-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O498581.2e-7143.59Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1[more]
A0A0N7F2971.3e-7044.87Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP... [more]
O498594.1e-6944.77Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1[more]
Q430681.2e-6844.62Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2[more]
Q9SZ463.8e-6745.36Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1J4B01.1e-13375.00cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111481116 PE=3 S... [more]
A0A6J1FKD22.7e-13274.04cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111446070 PE=3... [more]
A0A6J1F6977.9e-12469.28cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442562 PE=3... [more]
A0A5D3BG484.3e-12268.06Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3CMB94.3e-12268.06cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502574 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G31940.12.7e-6845.36cytochrome P450, family 82, subfamily C, polypeptide 4 [more]
AT4G31970.11.1e-6643.81cytochrome P450, family 82, subfamily C, polypeptide 2 [more]
AT4G31950.12.6e-5539.87cytochrome P450, family 82, subfamily C, polypeptide 3 [more]
AT3G25180.11.6e-4736.45cytochrome P450, family 82, subfamily G, polypeptide 1 [more]
AT3G25180.21.6e-4736.45cytochrome P450, family 82, subfamily G, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 65..84
score: 33.95
coord: 185..203
score: 30.32
coord: 89..110
score: 23.87
IPR001128Cytochrome P450PFAMPF00067p450coord: 36..306
e-value: 2.6E-31
score: 108.9
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..313
e-value: 9.1E-52
score: 178.1
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 30..296
NoneNo IPR availablePANTHERPTHR47947CYTOCHROME P450 82C3-RELATEDcoord: 32..312
NoneNo IPR availablePANTHERPTHR47947:SF26CYTOCHROME P450coord: 32..312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS026271.1MS026271.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen