MS025995 (gene) Bitter gourd (TR) v1

BLAST of MS025995 vs. NCBI nr
Match: XP_022142274.1 (pyruvate decarboxylase 1 [Momordica charantia])

HSP 1 Score: 523.5 bits (1347), Expect = 1.3e-144
Identity = 298/477 (62.47%), Postives = 298/477 (62.47%), Query Frame = 0

Query: 1   MEAANSIG--SASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNL 60
           MEAANSIG  SASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNL
Sbjct: 1   MEAANSIGSASASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNL 60

Query: 61  TLLDHLISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSE 120
           TLLDHLISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSE
Sbjct: 61  TLLDHLISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSE 120

Query: 121 NLPVICIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDT 180
           NLPVICIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDT
Sbjct: 121 NLPVICIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDT 180

Query: 181 AISTALKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNK 240
           AISTALKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNK
Sbjct: 181 AISTALKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNK 240

Query: 241 AVKPVIVGGPKLRVAKAQRA---------------------------------------- 299
           AVKPVIVGGPKLRVAKAQRA                                        
Sbjct: 241 AVKPVIVGGPKLRVAKAQRAFVELADASGYPIAVMPSGKGLVPEHHPQFIGTYWGAVSTS 300

BLAST of MS025995 vs. NCBI nr
Match: KZN07132.1 (hypothetical protein DCAR_007969 [Daucus carota subsp. sativus])

HSP 1 Score: 487.3 bits (1253), Expect = 1.0e-133
Identity = 246/333 (73.87%), Postives = 270/333 (81.08%), Query Frame = 0

Query: 1   MEAANSIGSASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTL 60
           M++AN +G+ S         P P+     +GTLGRH+ARRLV+IGV DVFSVPGDFNLTL
Sbjct: 1   MDSANQVGATS---------PAPITTGKFTGTLGRHIARRLVQIGVKDVFSVPGDFNLTL 60

Query: 61  LDHLISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENL 120
           LDHLI++PELNL+GCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENL
Sbjct: 61  LDHLINEPELNLVGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENL 120

Query: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAI 180
           PVICIVGGPNSNDYGTNRILHHTIGLPDF+QEL+CF+TVTC QA+VN+L+DAHELID AI
Sbjct: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCAQAVVNNLEDAHELIDNAI 180

Query: 181 STALKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAV 240
           STALKESKPVYISISCNLPGI HPTF+ DPVP+C+APK+SNQ GLEAAVEATA  LNKAV
Sbjct: 181 STALKESKPVYISISCNLPGIPHPTFSIDPVPYCIAPKVSNQLGLEAAVEATAELLNKAV 240

Query: 241 KPVIVGGPKLRVAKAQRAF-----------------------------------EFQMQY 299
           KPVIVGGPKLRV KAQ+AF                                   EFQMQY
Sbjct: 241 KPVIVGGPKLRVGKAQKAFEEFANASGGDTAVIAETGDSWFNCQKLRLPENCGYEFQMQY 300

BLAST of MS025995 vs. NCBI nr
Match: KAE7995812.1 (hypothetical protein FH972_000579 [Carpinus fangiana])

HSP 1 Score: 482.3 bits (1240), Expect = 3.2e-132
Identity = 255/369 (69.11%), Postives = 273/369 (73.98%), Query Frame = 0

Query: 1   MEAANSIGSASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTL 60
           MEAAN +G  S +HP   P P PV P AS GTLGRHLARRLVEIGV DVFSVPGDFNL L
Sbjct: 1   MEAANHVG--SGAHP--CPAPEPVQPIASIGTLGRHLARRLVEIGVRDVFSVPGDFNLAL 60

Query: 61  LDHLISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENL 120
           LDHLI++PELNL+GCCNELNAGYAADGYARAKGVGACVVTFTVGGLSV+NAIAGAYS+NL
Sbjct: 61  LDHLIAEPELNLVGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVINAIAGAYSDNL 120

Query: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAI 180
           PVICIVGGPNSNDYGTNRILHHTIGLPDFTQEL+CF+TVTC QA+VN+LDDAHE IDTAI
Sbjct: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFTQELRCFQTVTCAQAVVNNLDDAHEQIDTAI 180

Query: 181 STALKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAV 240
           STALKESKPVYISISCNLPGI H TFAR+PVPF LAP  SNQ GLEAAVEAT  FL+KAV
Sbjct: 181 STALKESKPVYISISCNLPGIPHSTFAREPVPFSLAPNASNQLGLEAAVEATVEFLHKAV 240

Query: 241 KPVIVGGPKLRVAKAQRAF----------------------------------------- 299
           KPV+VGGPKLRVAKAQ+AF                                         
Sbjct: 241 KPVLVGGPKLRVAKAQKAFVELADASGHAYSYYVLCVLPERDLLVKCLIWVGTYGLVYIC 300

BLAST of MS025995 vs. NCBI nr
Match: THG15477.1 (hypothetical protein TEA_020710 [Camellia sinensis var. sinensis])

HSP 1 Score: 477.2 bits (1227), Expect = 1.0e-130
Identity = 230/290 (79.31%), Postives = 259/290 (89.31%), Query Frame = 0

Query: 27  NASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTLLDHLISDPELNLIGCCNELNAGYAAD 86
           NA+  TLGRHLARRLV+IGV DVFSVPGDFNLTLLDHL+++P LNLIGCCNELNAGYAAD
Sbjct: 39  NAADSTLGRHLARRLVQIGVSDVFSVPGDFNLTLLDHLLAEPGLNLIGCCNELNAGYAAD 98

Query: 87  GYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGL 146
           G+AR++GVGACVVTFTVGGLSV+NAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGL
Sbjct: 99  GFARSRGVGACVVTFTVGGLSVINAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGL 158

Query: 147 PDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAISTALKESKPVYISISCNLPGIAHPTF 206
           PDF+QEL+CF+TVTC+QA+VN+L+DAHELID AISTALKESKPVYIS+SCNLP I HPTF
Sbjct: 159 PDFSQELRCFQTVTCYQAVVNNLEDAHELIDNAISTALKESKPVYISVSCNLPAIPHPTF 218

Query: 207 ARDPVPFCLAPKISNQCGLEAAVEATANFLNKAVKPVIVGGPKLRVAKAQRAFEFQMQYG 266
           + +PVPF ++P++SNQ GLEAAVEA   FLNKAVKPV+V GPKLRVAKA  AF FQMQYG
Sbjct: 219 SCEPVPFSISPRLSNQMGLEAAVEAATEFLNKAVKPVLVAGPKLRVAKASDAF-FQMQYG 278

Query: 267 SIGWSVGATLGYAQATKNKRIIACIGDGSFQV----------CKPNTFLF 307
           SIGW+VGATLGYAQA +NKR+IACIGDGSFQV          C+  T +F
Sbjct: 279 SIGWAVGATLGYAQAARNKRVIACIGDGSFQVTAQDVSTMLRCEQRTIIF 327

BLAST of MS025995 vs. NCBI nr
Match: KAF3778921.1 (Pyruvate decarboxylase 4 [Nymphaea thermarum])

HSP 1 Score: 472.6 bits (1215), Expect = 2.5e-129
Identity = 238/318 (74.84%), Postives = 264/318 (83.02%), Query Frame = 0

Query: 4   ANSIGSASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTLLDH 63
           +N +G+   S+ + A +   V P +S  TLGRHLARRLV++GV DVFSVPGDFNLTLLDH
Sbjct: 15  SNDVGAPPCSN-SVAVVQNSVCPVSSDSTLGRHLARRLVQVGVSDVFSVPGDFNLTLLDH 74

Query: 64  LISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVI 123
           LI++P LN+IGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVI
Sbjct: 75  LIAEPGLNVIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVI 134

Query: 124 CIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAISTA 183
           CIVGGPNSNDYGTNRILHHTIGLPDFTQEL+CF+TVTCFQA+VN+LDDAHELIDTA+STA
Sbjct: 135 CIVGGPNSNDYGTNRILHHTIGLPDFTQELRCFQTVTCFQAVVNNLDDAHELIDTAVSTA 194

Query: 184 LKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAVKPV 243
           LKESKPVYISI CNL  I HPTF+R+PVPF LAPK+SNQ GLEAAVEAT  FLNKAVKPV
Sbjct: 195 LKESKPVYISIGCNLSHIPHPTFSREPVPFFLAPKLSNQMGLEAAVEATVEFLNKAVKPV 254

Query: 244 IVGGPKLRVAKAQR-----------------------AFEFQMQYGSIGWSVGATLGYAQ 299
           +VGGPKLR   +                          +EFQMQYGSIGWSVGATLGYAQ
Sbjct: 255 MVGGPKLREMLSSDTAVISETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQ 314

BLAST of MS025995 vs. ExPASy Swiss-Prot
Match: P51850 (Pyruvate decarboxylase 1 OS=Pisum sativum OX=3888 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 9.7e-124
Identity = 216/259 (83.40%), Postives = 234/259 (90.35%), Query Frame = 0

Query: 1   MEAANSIGSASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTL 60
           ME      + S   PTSAP  +P+ P++  GT+GRHLARRLVEIGV DVFSVPGDFNLTL
Sbjct: 1   METETETPNGSTPCPTSAPSAIPLRPSSCDGTMGRHLARRLVEIGVRDVFSVPGDFNLTL 60

Query: 61  LDHLISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENL 120
           LDHLI++PELNL+GCCNELNAGYAADGY RAKGVGACVVTFTVGGLS+LNAIAGAYSENL
Sbjct: 61  LDHLIAEPELNLVGCCNELNAGYAADGYGRAKGVGACVVTFTVGGLSILNAIAGAYSENL 120

Query: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAI 180
           PVICIVGGPNSNDYGTNRILHHTIGLPDF+QELQCF+T+TCFQA+VN+LDDAHELIDTAI
Sbjct: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFSQELQCFQTITCFQAVVNNLDDAHELIDTAI 180

Query: 181 STALKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAV 240
           STALKESKPVYISI CNLP I HPTFARDPVPF LAP++SNQ GLEAAVE  A FLNKAV
Sbjct: 181 STALKESKPVYISIGCNLPAIPHPTFARDPVPFFLAPRVSNQAGLEAAVEEAAAFLNKAV 240

Query: 241 KPVIVGGPKLRVAKAQRAF 260
           KPVIVGGPKLRVAKAQ+AF
Sbjct: 241 KPVIVGGPKLRVAKAQKAF 259

BLAST of MS025995 vs. ExPASy Swiss-Prot
Match: A2XFI3 (Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=2)

HSP 1 Score: 407.1 bits (1045), Expect = 1.7e-112
Identity = 195/245 (79.59%), Postives = 222/245 (90.61%), Query Frame = 0

Query: 15  PTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTLLDHLISDPELNLIG 74
           P ++  P PV  +A   +LGRHLARRLV++GV DVF+VPGDFNLTLLDHLI++P L L+G
Sbjct: 30  PMTSARPAPV--SAGEASLGRHLARRLVQVGVSDVFAVPGDFNLTLLDHLIAEPGLRLVG 89

Query: 75  CCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDY 134
           CCNELNAGYAADGYARA+GVGAC VTFTVGGLSVLNAIAGAYSENLPVICI GGPNSNDY
Sbjct: 90  CCNELNAGYAADGYARARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIAGGPNSNDY 149

Query: 135 GTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAISTALKESKPVYISI 194
           GTNRILHHTIGLPDF+QEL+CF+TVTC QA+V +L+DAHE IDTAI+TAL+ESKPVY+SI
Sbjct: 150 GTNRILHHTIGLPDFSQELRCFQTVTCHQAVVTNLEDAHEQIDTAIATALRESKPVYLSI 209

Query: 195 SCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAVKPVIVGGPKLRVAK 254
           SCNLPG+ HPTF+RDPVPF LAP++SN+ GLEAAVEAT  FLNKAVKPV+VGGPKLRVAK
Sbjct: 210 SCNLPGLPHPTFSRDPVPFFLAPRLSNKMGLEAAVEATVEFLNKAVKPVLVGGPKLRVAK 269

Query: 255 AQRAF 260
           A +AF
Sbjct: 270 AGKAF 272

HSP 2 Score: 78.6 bits (192), Expect = 1.4e-13
Identity = 37/58 (63.79%), Postives = 42/58 (72.41%), Query Frame = 0

Query: 259 FEFQMQYGSIGWSVGATLGYAQATKNKRIIACIGDGSFQV----------CKPNTFLF 307
           +EFQMQYGSIGWSVGA LGYAQ  K+KR+IACIGDGSFQV          C  N+ +F
Sbjct: 449 YEFQMQYGSIGWSVGALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIF 506

BLAST of MS025995 vs. ExPASy Swiss-Prot
Match: Q10MW3 (Pyruvate decarboxylase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC2 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 1.7e-112
Identity = 195/245 (79.59%), Postives = 222/245 (90.61%), Query Frame = 0

Query: 15  PTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTLLDHLISDPELNLIG 74
           P ++  P PV  +A   +LGRHLARRLV++GV DVF+VPGDFNLTLLDHLI++P L L+G
Sbjct: 30  PMTSARPAPV--SAGEASLGRHLARRLVQVGVSDVFAVPGDFNLTLLDHLIAEPGLRLVG 89

Query: 75  CCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDY 134
           CCNELNAGYAADGYARA+GVGAC VTFTVGGLSVLNAIAGAYSENLPVICI GGPNSNDY
Sbjct: 90  CCNELNAGYAADGYARARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIAGGPNSNDY 149

Query: 135 GTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAISTALKESKPVYISI 194
           GTNRILHHTIGLPDF+QEL+CF+TVTC QA+V +L+DAHE IDTAI+TAL+ESKPVY+SI
Sbjct: 150 GTNRILHHTIGLPDFSQELRCFQTVTCHQAVVTNLEDAHEQIDTAIATALRESKPVYLSI 209

Query: 195 SCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAVKPVIVGGPKLRVAK 254
           SCNLPG+ HPTF+RDPVPF LAP++SN+ GLEAAVEAT  FLNKAVKPV+VGGPKLRVAK
Sbjct: 210 SCNLPGLPHPTFSRDPVPFFLAPRLSNKMGLEAAVEATVEFLNKAVKPVLVGGPKLRVAK 269

Query: 255 AQRAF 260
           A +AF
Sbjct: 270 AGKAF 272

HSP 2 Score: 78.6 bits (192), Expect = 1.4e-13
Identity = 37/58 (63.79%), Postives = 42/58 (72.41%), Query Frame = 0

Query: 259 FEFQMQYGSIGWSVGATLGYAQATKNKRIIACIGDGSFQV----------CKPNTFLF 307
           +EFQMQYGSIGWSVGA LGYAQ  K+KR+IACIGDGSFQV          C  N+ +F
Sbjct: 449 YEFQMQYGSIGWSVGALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIF 506

BLAST of MS025995 vs. ExPASy Swiss-Prot
Match: O82647 (Pyruvate decarboxylase 1 OS=Arabidopsis thaliana OX=3702 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 5.5e-111
Identity = 199/254 (78.35%), Postives = 224/254 (88.19%), Query Frame = 0

Query: 6   SIGSASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTLLDHLI 65
           ++ +   S P+SA     +  N    TLGRHLARRLV+ GV DVFSVPGDFNLTLLDHL+
Sbjct: 25  TVATIHNSVPSSA-----ITINYCDATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLM 84

Query: 66  SDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVICI 125
           ++P+LNLIGCCNELNAGYAADGYAR++GVGACVVTFTVGGLSVLNAIAGAYSENLP+ICI
Sbjct: 85  AEPDLNLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICI 144

Query: 126 VGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAISTALK 185
           VGGPNSNDYGTNRILHHTIGLPDF+QEL+CF+TVTC+QA+VN+LDDAHE ID AISTALK
Sbjct: 145 VGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALK 204

Query: 186 ESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAVKPVIV 245
           ESKPVYIS+SCNL  I H TF+RDPVPF LAP++SN+ GLEAAVEAT  FLNKAVKPV+V
Sbjct: 205 ESKPVYISVSCNLAAIPHHTFSRDPVPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMV 264

Query: 246 GGPKLRVAKAQRAF 260
           GGPKLRVAKA  AF
Sbjct: 265 GGPKLRVAKACDAF 273

HSP 2 Score: 74.3 bits (181), Expect = 2.6e-12
Identity = 36/50 (72.00%), Postives = 42/50 (84.00%), Query Frame = 0

Query: 249 KLRVAKAQRAFEFQMQYGSIGWSVGATLGYAQATKNKRIIACIGDGSFQV 299
           KL++ K    +EFQMQYGSIGWSVGATLGYAQA+  KR++A IGDGSFQV
Sbjct: 441 KLKLPKG-CGYEFQMQYGSIGWSVGATLGYAQASPEKRVLAFIGDGSFQV 489

BLAST of MS025995 vs. ExPASy Swiss-Prot
Match: P51846 (Pyruvate decarboxylase 2 OS=Nicotiana tabacum OX=4097 GN=PDC2 PE=2 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 5.5e-111
Identity = 192/231 (83.12%), Postives = 214/231 (92.64%), Query Frame = 0

Query: 29  SSGTLGRHLARRLVEIGVGDVFSVPGDFNLTLLDHLISDPELNLIGCCNELNAGYAADGY 88
           +  TLGRHLARRLVEIG+ DVFSVPGDFNLTLLDHLI++P L  IGCCNELNAGYAADGY
Sbjct: 24  TDATLGRHLARRLVEIGIQDVFSVPGDFNLTLLDHLIAEPRLKNIGCCNELNAGYAADGY 83

Query: 89  ARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPD 148
           ARA+GVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPN+NDYGTNRILHHTIGLPD
Sbjct: 84  ARARGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNTNDYGTNRILHHTIGLPD 143

Query: 149 FTQELQCFRTVTCFQAIVNDLDDAHELIDTAISTALKESKPVYISISCNLPGIAHPTFAR 208
           F+QEL+CF+TVTC+QA+VN+LDDAHE ID AISTALKESKPVYISISCNLP I HPTF+R
Sbjct: 144 FSQELRCFQTVTCYQAVVNNLDDAHEQIDRAISTALKESKPVYISISCNLPAIPHPTFSR 203

Query: 209 DPVPFCLAPKISNQCGLEAAVEATANFLNKAVKPVIVGGPKLRVAKAQRAF 260
           DP+PF L+P++SN+ GLEAAV+A   FL+KAVKPV++GGPKLRVAKA  AF
Sbjct: 204 DPIPFSLSPRLSNKRGLEAAVDAAVTFLSKAVKPVMIGGPKLRVAKACDAF 254

HSP 2 Score: 76.6 bits (187), Expect = 5.3e-13
Identity = 33/40 (82.50%), Postives = 37/40 (92.50%), Query Frame = 0

Query: 259 FEFQMQYGSIGWSVGATLGYAQATKNKRIIACIGDGSFQV 299
           +EFQMQYGSIGWSVGATLGYAQ+   KR+I+CIGDGSFQV
Sbjct: 457 YEFQMQYGSIGWSVGATLGYAQSVPKKRVISCIGDGSFQV 496

BLAST of MS025995 vs. ExPASy TrEMBL
Match: A0A6J1CL45 (Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111012435 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 6.1e-145
Identity = 298/477 (62.47%), Postives = 298/477 (62.47%), Query Frame = 0

Query: 1   MEAANSIG--SASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNL 60
           MEAANSIG  SASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNL
Sbjct: 1   MEAANSIGSASASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNL 60

Query: 61  TLLDHLISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSE 120
           TLLDHLISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSE
Sbjct: 61  TLLDHLISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSE 120

Query: 121 NLPVICIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDT 180
           NLPVICIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDT
Sbjct: 121 NLPVICIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDT 180

Query: 181 AISTALKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNK 240
           AISTALKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNK
Sbjct: 181 AISTALKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNK 240

Query: 241 AVKPVIVGGPKLRVAKAQRA---------------------------------------- 299
           AVKPVIVGGPKLRVAKAQRA                                        
Sbjct: 241 AVKPVIVGGPKLRVAKAQRAFVELADASGYPIAVMPSGKGLVPEHHPQFIGTYWGAVSTS 300

BLAST of MS025995 vs. ExPASy TrEMBL
Match: A0A166EYH6 (Pyruvate decarboxylase OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_007969 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 4.8e-134
Identity = 246/333 (73.87%), Postives = 270/333 (81.08%), Query Frame = 0

Query: 1   MEAANSIGSASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTL 60
           M++AN +G+ S         P P+     +GTLGRH+ARRLV+IGV DVFSVPGDFNLTL
Sbjct: 1   MDSANQVGATS---------PAPITTGKFTGTLGRHIARRLVQIGVKDVFSVPGDFNLTL 60

Query: 61  LDHLISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENL 120
           LDHLI++PELNL+GCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENL
Sbjct: 61  LDHLINEPELNLVGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENL 120

Query: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAI 180
           PVICIVGGPNSNDYGTNRILHHTIGLPDF+QEL+CF+TVTC QA+VN+L+DAHELID AI
Sbjct: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCAQAVVNNLEDAHELIDNAI 180

Query: 181 STALKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAV 240
           STALKESKPVYISISCNLPGI HPTF+ DPVP+C+APK+SNQ GLEAAVEATA  LNKAV
Sbjct: 181 STALKESKPVYISISCNLPGIPHPTFSIDPVPYCIAPKVSNQLGLEAAVEATAELLNKAV 240

Query: 241 KPVIVGGPKLRVAKAQRAF-----------------------------------EFQMQY 299
           KPVIVGGPKLRV KAQ+AF                                   EFQMQY
Sbjct: 241 KPVIVGGPKLRVGKAQKAFEEFANASGGDTAVIAETGDSWFNCQKLRLPENCGYEFQMQY 300

BLAST of MS025995 vs. ExPASy TrEMBL
Match: A0A5N6Q986 (Pyruvate decarboxylase OS=Carpinus fangiana OX=176857 GN=FH972_000579 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.6e-132
Identity = 255/369 (69.11%), Postives = 273/369 (73.98%), Query Frame = 0

Query: 1   MEAANSIGSASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTL 60
           MEAAN +G  S +HP   P P PV P AS GTLGRHLARRLVEIGV DVFSVPGDFNL L
Sbjct: 1   MEAANHVG--SGAHP--CPAPEPVQPIASIGTLGRHLARRLVEIGVRDVFSVPGDFNLAL 60

Query: 61  LDHLISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENL 120
           LDHLI++PELNL+GCCNELNAGYAADGYARAKGVGACVVTFTVGGLSV+NAIAGAYS+NL
Sbjct: 61  LDHLIAEPELNLVGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVINAIAGAYSDNL 120

Query: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAI 180
           PVICIVGGPNSNDYGTNRILHHTIGLPDFTQEL+CF+TVTC QA+VN+LDDAHE IDTAI
Sbjct: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFTQELRCFQTVTCAQAVVNNLDDAHEQIDTAI 180

Query: 181 STALKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAV 240
           STALKESKPVYISISCNLPGI H TFAR+PVPF LAP  SNQ GLEAAVEAT  FL+KAV
Sbjct: 181 STALKESKPVYISISCNLPGIPHSTFAREPVPFSLAPNASNQLGLEAAVEATVEFLHKAV 240

Query: 241 KPVIVGGPKLRVAKAQRAF----------------------------------------- 299
           KPV+VGGPKLRVAKAQ+AF                                         
Sbjct: 241 KPVLVGGPKLRVAKAQKAFVELADASGHAYSYYVLCVLPERDLLVKCLIWVGTYGLVYIC 300

BLAST of MS025995 vs. ExPASy TrEMBL
Match: A0A4V3WP93 (Pyruvate decarboxylase OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_020710 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 5.0e-131
Identity = 230/290 (79.31%), Postives = 259/290 (89.31%), Query Frame = 0

Query: 27  NASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTLLDHLISDPELNLIGCCNELNAGYAAD 86
           NA+  TLGRHLARRLV+IGV DVFSVPGDFNLTLLDHL+++P LNLIGCCNELNAGYAAD
Sbjct: 39  NAADSTLGRHLARRLVQIGVSDVFSVPGDFNLTLLDHLLAEPGLNLIGCCNELNAGYAAD 98

Query: 87  GYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGL 146
           G+AR++GVGACVVTFTVGGLSV+NAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGL
Sbjct: 99  GFARSRGVGACVVTFTVGGLSVINAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGL 158

Query: 147 PDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAISTALKESKPVYISISCNLPGIAHPTF 206
           PDF+QEL+CF+TVTC+QA+VN+L+DAHELID AISTALKESKPVYIS+SCNLP I HPTF
Sbjct: 159 PDFSQELRCFQTVTCYQAVVNNLEDAHELIDNAISTALKESKPVYISVSCNLPAIPHPTF 218

Query: 207 ARDPVPFCLAPKISNQCGLEAAVEATANFLNKAVKPVIVGGPKLRVAKAQRAFEFQMQYG 266
           + +PVPF ++P++SNQ GLEAAVEA   FLNKAVKPV+V GPKLRVAKA  AF FQMQYG
Sbjct: 219 SCEPVPFSISPRLSNQMGLEAAVEAATEFLNKAVKPVLVAGPKLRVAKASDAF-FQMQYG 278

Query: 267 SIGWSVGATLGYAQATKNKRIIACIGDGSFQV----------CKPNTFLF 307
           SIGW+VGATLGYAQA +NKR+IACIGDGSFQV          C+  T +F
Sbjct: 279 SIGWAVGATLGYAQAARNKRVIACIGDGSFQVTAQDVSTMLRCEQRTIIF 327

BLAST of MS025995 vs. ExPASy TrEMBL
Match: A0A6J1IQV4 (Pyruvate decarboxylase OS=Cucurbita maxima OX=3661 GN=LOC111478567 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.2e-129
Identity = 273/475 (57.47%), Postives = 282/475 (59.37%), Query Frame = 0

Query: 1   MEAANSIGSASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTL 60
           M+AANSIG  SA HPTS  +P+P APN SSGTLG HLARRLVEIGV DVFSVPGDFNLTL
Sbjct: 1   MDAANSIG--SAVHPTS--VPVPAAPNVSSGTLGSHLARRLVEIGVSDVFSVPGDFNLTL 60

Query: 61  LDHLISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENL 120
           LDH +S+P LNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENL
Sbjct: 61  LDHFVSEPGLNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENL 120

Query: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAI 180
           PVICIVGGPNSNDYGTNRILHHTIGLPDFTQEL+CF+TVTC+QAIVNDLDDAHELIDTAI
Sbjct: 121 PVICIVGGPNSNDYGTNRILHHTIGLPDFTQELRCFQTVTCYQAIVNDLDDAHELIDTAI 180

Query: 181 STALKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAV 240
           STALKESKPVYISISCNLPGI HPTFARDPVPF LAPKISNQ GLEAAVEATANFLN AV
Sbjct: 181 STALKESKPVYISISCNLPGIPHPTFARDPVPFILAPKISNQWGLEAAVEATANFLNNAV 240

Query: 241 KPVIVGGPKLRVAKAQRAF----------------------------------------- 299
           KPVIVGGPKLR AKAQRAF                                         
Sbjct: 241 KPVIVGGPKLRAAKAQRAFLELADASGYPIAVMPSGKGMVPEHHPQFIGTYWGAVSSSFC 300

BLAST of MS025995 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 402.1 bits (1032), Expect = 3.9e-112
Identity = 199/254 (78.35%), Postives = 224/254 (88.19%), Query Frame = 0

Query: 6   SIGSASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTLLDHLI 65
           ++ +   S P+SA     +  N    TLGRHLARRLV+ GV DVFSVPGDFNLTLLDHL+
Sbjct: 25  TVATIHNSVPSSA-----ITINYCDATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLM 84

Query: 66  SDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVICI 125
           ++P+LNLIGCCNELNAGYAADGYAR++GVGACVVTFTVGGLSVLNAIAGAYSENLP+ICI
Sbjct: 85  AEPDLNLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICI 144

Query: 126 VGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAISTALK 185
           VGGPNSNDYGTNRILHHTIGLPDF+QEL+CF+TVTC+QA+VN+LDDAHE ID AISTALK
Sbjct: 145 VGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALK 204

Query: 186 ESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAVKPVIV 245
           ESKPVYIS+SCNL  I H TF+RDPVPF LAP++SN+ GLEAAVEAT  FLNKAVKPV+V
Sbjct: 205 ESKPVYISVSCNLAAIPHHTFSRDPVPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMV 264

Query: 246 GGPKLRVAKAQRAF 260
           GGPKLRVAKA  AF
Sbjct: 265 GGPKLRVAKACDAF 273

HSP 2 Score: 74.3 bits (181), Expect = 1.9e-13
Identity = 36/50 (72.00%), Postives = 42/50 (84.00%), Query Frame = 0

Query: 249 KLRVAKAQRAFEFQMQYGSIGWSVGATLGYAQATKNKRIIACIGDGSFQV 299
           KL++ K    +EFQMQYGSIGWSVGATLGYAQA+  KR++A IGDGSFQV
Sbjct: 441 KLKLPKG-CGYEFQMQYGSIGWSVGATLGYAQASPEKRVLAFIGDGSFQV 489

BLAST of MS025995 vs. TAIR 10
Match: AT5G54960.1 (pyruvate decarboxylase-2 )

HSP 1 Score: 400.2 bits (1027), Expect = 1.5e-111
Identity = 235/468 (50.21%), Postives = 256/468 (54.70%), Query Frame = 0

Query: 8   GSASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTLLDHLISD 67
           G  S    TS      V+P     TLGR+LARRLVEIGV DVFSVPGDFNLTLLDHLI++
Sbjct: 24  GGVSTVQNTSPLHSTTVSP--CDATLGRYLARRLVEIGVTDVFSVPGDFNLTLLDHLIAE 83

Query: 68  PELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVG 127
           P L LIGCCNELNAGYAADGYAR++GVGACVVTFTVGGLSVLNAIAGAYSENLP+ICIVG
Sbjct: 84  PNLKLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVG 143

Query: 128 GPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAISTALKES 187
           GPNSNDYGTNRILHHTIGLPDFTQEL+CF+ VTCFQA++N+L++AHELIDTAISTALKES
Sbjct: 144 GPNSNDYGTNRILHHTIGLPDFTQELRCFQAVTCFQAVINNLEEAHELIDTAISTALKES 203

Query: 188 KPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAVKPVIVGG 247
           KPVYISISCNLP I  PTF+R PVPF L  K+SNQ GL+AAVEA A FLNKAVKPV+VGG
Sbjct: 204 KPVYISISCNLPAIPLPTFSRHPVPFMLPMKVSNQIGLDAAVEAAAEFLNKAVKPVLVGG 263

Query: 248 PKLRVAKAQRAF------------------------------------------------ 299
           PK+RVAKA  AF                                                
Sbjct: 264 PKMRVAKAADAFVELADASGYGLAVMPSAKGQVPEHHKHFIGTYWGAVSTAFCAEIVESA 323

BLAST of MS025995 vs. TAIR 10
Match: AT5G01320.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 399.8 bits (1026), Expect = 1.9e-111
Identity = 199/251 (79.28%), Postives = 220/251 (87.65%), Query Frame = 0

Query: 9   SASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTLLDHLISDP 68
           +A A+   SAP+        S  TLGRHL+RRLV+ GV DVFSVPGDFNLTLLDHLI++P
Sbjct: 24  NAVATIQDSAPI-----TTTSESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEP 83

Query: 69  ELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGG 128
           ELN IGCCNELNAGYAADGYAR++GVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGG
Sbjct: 84  ELNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGG 143

Query: 129 PNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAISTALKESK 188
           PNSND+GTNRILHHTIGLPDF+QEL+CF+TVTC+QA+VN+L+DAHE ID AI+TALKESK
Sbjct: 144 PNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALKESK 203

Query: 189 PVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAVKPVIVGGP 248
           PVYISISCNL    HPTFARDPVPF L P++SN  GLEAAVEAT  FLNKAVKPV+VGGP
Sbjct: 204 PVYISISCNLAATPHPTFARDPVPFDLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGP 263

Query: 249 KLRVAKAQRAF 260
           KLRVAKA  AF
Sbjct: 264 KLRVAKASEAF 269

HSP 2 Score: 74.7 bits (182), Expect = 1.4e-13
Identity = 36/50 (72.00%), Postives = 42/50 (84.00%), Query Frame = 0

Query: 249 KLRVAKAQRAFEFQMQYGSIGWSVGATLGYAQATKNKRIIACIGDGSFQV 299
           KL++ K    +EFQMQYGSIGWSVGATLGYAQAT  KR+++ IGDGSFQV
Sbjct: 437 KLKLPKG-CGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQV 485

BLAST of MS025995 vs. TAIR 10
Match: AT5G01330.1 (pyruvate decarboxylase-3 )

HSP 1 Score: 386.7 bits (992), Expect = 1.7e-107
Identity = 192/256 (75.00%), Postives = 222/256 (86.72%), Query Frame = 0

Query: 4   ANSIGSASASHPTSAPLPLPVAPNASSGTLGRHLARRLVEIGVGDVFSVPGDFNLTLLDH 63
           +N + +   S PT+A +       +S+ TLGRHL+RRLV+ GV D+F+VPGDFNL+LLD 
Sbjct: 8   SNGVATIQDSAPTAATI-----LGSSAATLGRHLSRRLVQAGVTDIFTVPGDFNLSLLDQ 67

Query: 64  LISDPELNLIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVI 123
           LI++PELN IGCCNELNAGYAADGYAR++GVGACVVTFTVGGLSVLNAIAGAYSENLPVI
Sbjct: 68  LIANPELNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVI 127

Query: 124 CIVGGPNSNDYGTNRILHHTIGLPDFTQELQCFRTVTCFQAIVNDLDDAHELIDTAISTA 183
           CIVGGPNSND+GTNRILHHTIGLPDF+QEL+CF+TVTC+QA+VN L+DAHE ID AI+TA
Sbjct: 128 CIVGGPNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNHLEDAHEQIDKAIATA 187

Query: 184 LKESKPVYISISCNLPGIAHPTFARDPVPFCLAPKISNQCGLEAAVEATANFLNKAVKPV 243
           L+ESKPVYISISCNL  I HPTFA  PVPF L P++SN+  LEAAVEAT  FLNKAVKPV
Sbjct: 188 LRESKPVYISISCNLAAIPHPTFASYPVPFDLTPRLSNKDCLEAAVEATLEFLNKAVKPV 247

Query: 244 IVGGPKLRVAKAQRAF 260
           +VGGPKLRVAKA+ AF
Sbjct: 248 MVGGPKLRVAKARDAF 258

HSP 2 Score: 74.7 bits (182), Expect = 1.4e-13
Identity = 36/50 (72.00%), Postives = 42/50 (84.00%), Query Frame = 0

Query: 249 KLRVAKAQRAFEFQMQYGSIGWSVGATLGYAQATKNKRIIACIGDGSFQV 299
           KL++ K    +EFQMQYGSIGWSVGATLGYAQAT  KR+++ IGDGSFQV
Sbjct: 426 KLKLPKG-CGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQV 474