MS025941 (gene) Bitter gourd (TR) v1

Overview
NameMS025941
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncytochrome P450 CYP736A12-like
Locationscaffold35: 112398 .. 113987 (+)
RNA-Seq ExpressionMS025941
SyntenyMS025941
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTTGGATTTGGGCCACCATTGCACTTCTTCTTCTTCTTGCTAATTACCTCTTACAGCCATGGCTGTTCAACAGCAAGAAATTGCCTCCTGGCCCAAAAGGGTTTCCCATTCTGGGAAGCCTTAATCTTTTGGGAAAATTCCCTCACAGAGATCTTCAAAAATTGTCCCAAAAATATGGTCCAATCATGCACATGCGGCTGGGACTTGTAAACACCATTGTTGTCTCTTCCCCACAAGCTGCTGAGCTTTTCCTCAAAACCCATGACCTTGTTTTTGCAAGCAGGCCACTTACTATGGTCTCTAAGTTCCTCTCTTATGGGCAAAAGAATCTGGTTTTTGCTCAATATGGATCTTACTGGCGTAATGTGCGCAAAATGTGTACACTTGAATTGCTAAGTAACCTCAAGATCAATTCTTTCAAGTCCATGAGAAAGGAAGAGCTTGGATTGCTGATTGATCGTGTTCGAGAGAAGGCTAATGATGGTGCTGTGGTTGATCTTAGTTCTGAAGTATCGTCTCTAAGCACAGATATGACTTGTTTGATGGTGTTTGGAAGGAAATTTTCGGATGAAGAGTTTGATGATAGGGGTTTCAAGACTATGATTCAAGAGGTGATGCAATTGGTTGCTGCTCCTAATTTGGGAGATTTTAATATTCCTTTTGTTGCAATGTTTGATCTTCAAGGGCTGACTCGTCGGATGAAAAATGTTAGCAAAGTGTTTGATGAGTTCTTTGAAAGGATTATTGATGAGCATCTTAAGCCAATGGGTGCAAAAAGAAGTAAGGATTTTGTAGATGTCATGCTAGATCTAATGGGGTCTGAAGAAACTGAGTACCAAATTGATCGGTCTAGCATCAAAGCAATAATGTTGGTAAGTGAACCAAAAATGATATTTTTATGAAAGCTATAAAAGAGATTGTGACGTGTTTATAAATGGTTAATTGTACTTTTACAGGATATACTTGGTGCTGCAATGGACACTTCAGCTACGGTAATTGGATGGGCGCTACCTGAACTCATAAAGAACCCACAGGTAATGAAGAAAGTGCAAGATGAATTGGAAAAAGTAGTGGGCTTGGATAGGATGGTGGAAGAATCAGACTTGGTAAATTTAGAATATCTGGACATGGTCATTAAGGAAACTCTTAGGTTGTATCCGCCTGTTCCGTTGTTAGTTCCACATGAATCTCTCGAGGATTGTGTTGTCAATGACTTTCATATTCCAAAGAAATCGCGCGTAATTGTGAATGCATGGGCAATTGGACGAAATCCTAGTACTTGGACTGATCCAGAGAAGTTCTACCCTGAGAGGTTTGTTGATAGCCAAGTGGACGTGAGAGGGAGAGATTTTCAATTAATTCCATTTGGAGCTGGTCGAAGAGGTTGTCCTGGAATACAACTTGGTCTAACTGTGGTTCGTTTGATACTAGCCCAACTCATGCATTGTTTTGATTGGAAGCTTCCAAATGATATGTTGCCAGCTCAATTGGATATGACAGAAGAATTTGGTTTAACTTGTCCTAGAGCTCAAGATCTTATAGTTACTCCTATTTATCGTATTCATTCTGATGCCAAATTCAATTAA

mRNA sequence

ATGCCTTGGATTTGGGCCACCATTGCACTTCTTCTTCTTCTTGCTAATTACCTCTTACAGCCATGGCTGTTCAACAGCAAGAAATTGCCTCCTGGCCCAAAAGGGTTTCCCATTCTGGGAAGCCTTAATCTTTTGGGAAAATTCCCTCACAGAGATCTTCAAAAATTGTCCCAAAAATATGGTCCAATCATGCACATGCGGCTGGGACTTGTAAACACCATTGTTGTCTCTTCCCCACAAGCTGCTGAGCTTTTCCTCAAAACCCATGACCTTGTTTTTGCAAGCAGGCCACTTACTATGGTCTCTAAGTTCCTCTCTTATGGGCAAAAGAATCTGGTTTTTGCTCAATATGGATCTTACTGGCGTAATGTGCGCAAAATGTGTACACTTGAATTGCTAAGTAACCTCAAGATCAATTCTTTCAAGTCCATGAGAAAGGAAGAGCTTGGATTGCTGATTGATCGTGTTCGAGAGAAGGCTAATGATGGTGCTGTGGTTGATCTTAGTTCTGAAGTATCGTCTCTAAGCACAGATATGACTTGTTTGATGGTGTTTGGAAGGAAATTTTCGGATGAAGAGTTTGATGATAGGGGTTTCAAGACTATGATTCAAGAGGTGATGCAATTGGTTGCTGCTCCTAATTTGGGAGATTTTAATATTCCTTTTGTTGCAATGTTTGATCTTCAAGGGCTGACTCGTCGGATGAAAAATGTTAGCAAAGTGTTTGATGAGTTCTTTGAAAGGATTATTGATGAGCATCTTAAGCCAATGGGTGCAAAAAGAAGTAAGGATTTTGTAGATGTCATGCTAGATCTAATGGGGTCTGAAGAAACTGAGTACCAAATTGATCGGTCTAGCATCAAAGCAATAATGTTGGATATACTTGGTGCTGCAATGGACACTTCAGCTACGCTTCCAAATGATATGTTGCCAGCTCAATTGGATATGACAGAAGAATTTGGTTTAACTTGTCCTAGAGCTCAAGATCTTATAGTTACTCCTATTTATCGTATTCATTCTGATGCCAAATTCAATTAA

Coding sequence (CDS)

ATGCCTTGGATTTGGGCCACCATTGCACTTCTTCTTCTTCTTGCTAATTACCTCTTACAGCCATGGCTGTTCAACAGCAAGAAATTGCCTCCTGGCCCAAAAGGGTTTCCCATTCTGGGAAGCCTTAATCTTTTGGGAAAATTCCCTCACAGAGATCTTCAAAAATTGTCCCAAAAATATGGTCCAATCATGCACATGCGGCTGGGACTTGTAAACACCATTGTTGTCTCTTCCCCACAAGCTGCTGAGCTTTTCCTCAAAACCCATGACCTTGTTTTTGCAAGCAGGCCACTTACTATGGTCTCTAAGTTCCTCTCTTATGGGCAAAAGAATCTGGTTTTTGCTCAATATGGATCTTACTGGCGTAATGTGCGCAAAATGTGTACACTTGAATTGCTAAGTAACCTCAAGATCAATTCTTTCAAGTCCATGAGAAAGGAAGAGCTTGGATTGCTGATTGATCGTGTTCGAGAGAAGGCTAATGATGGTGCTGTGGTTGATCTTAGTTCTGAAGTATCGTCTCTAAGCACAGATATGACTTGTTTGATGGTGTTTGGAAGGAAATTTTCGGATGAAGAGTTTGATGATAGGGGTTTCAAGACTATGATTCAAGAGGTGATGCAATTGGTTGCTGCTCCTAATTTGGGAGATTTTAATATTCCTTTTGTTGCAATGTTTGATCTTCAAGGGCTGACTCGTCGGATGAAAAATGTTAGCAAAGTGTTTGATGAGTTCTTTGAAAGGATTATTGATGAGCATCTTAAGCCAATGGGTGCAAAAAGAAGTAAGGATTTTGTAGATGTCATGCTAGATCTAATGGGGTCTGAAGAAACTGAGTACCAAATTGATCGGTCTAGCATCAAAGCAATAATGTTGGATATACTTGGTGCTGCAATGGACACTTCAGCTACGCTTCCAAATGATATGTTGCCAGCTCAATTGGATATGACAGAAGAATTTGGTTTAACTTGTCCTAGAGCTCAAGATCTTATAGTTACTCCTATTTATCGTATTCATTCTGATGCCAAATTCAATTAA

Protein sequence

MPWIWATIALLLLLANYLLQPWLFNSKKLPPGPKGFPILGSLNLLGKFPHRDLQKLSQKYGPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDMTCLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMKNVSKVFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDILGAAMDTSATLPNDMLPAQLDMTEEFGLTCPRAQDLIVTPIYRIHSDAKFN
Homology
BLAST of MS025941 vs. NCBI nr
Match: XP_022132668.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 613.2 bits (1580), Expect = 1.4e-171
Identity = 341/501 (68.06%), Postives = 341/501 (68.06%), Query Frame = 0

Query: 1   MPWIWATIALLLLLANYLLQPWLFNSKKLPPGPKGFPILGSLNLLGKFPHRDLQKLSQKY 60
           MPWIWATIALLLLLANYLLQPWLFNSKKLPPGPKGFPILG LNLLGKFPHRDLQKLSQKY
Sbjct: 1   MPWIWATIALLLLLANYLLQPWLFNSKKLPPGPKGFPILGCLNLLGKFPHRDLQKLSQKY 60

Query: 61  GPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSY 120
           GPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSY
Sbjct: 61  GPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSY 120

Query: 121 WRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDMT 180
           WRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDMT
Sbjct: 121 WRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDMT 180

Query: 181 CLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMKNVSK 240
           CLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVA FDLQGLTRRMKNVSK
Sbjct: 181 CLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVATFDLQGLTRRMKNVSK 240

Query: 241 VFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDILGAAMD 300
           VFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDILGAAMD
Sbjct: 241 VFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDILGAAMD 300

Query: 301 TSAT-------------------------------------------------------- 346
           TSAT                                                        
Sbjct: 301 TSATVIGWALPELIKNPQVMKKVQDELEKVVGLDRMVEESDLVNLEYLDMVIKETLRLYP 360

BLAST of MS025941 vs. NCBI nr
Match: XP_022950766.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 501.5 bits (1290), Expect = 5.7e-138
Identity = 257/317 (81.07%), Postives = 285/317 (89.91%), Query Frame = 0

Query: 1   MPWIWATIALLLLLANYLLQPWLFN----SKKLPPGPKGFPILGSLNLLGKFPHRDLQKL 60
           MPWIWAT+A LLLL++YL Q W FN     KKLPPGPKGFPI+GSLNLL +FPHRDL +L
Sbjct: 1   MPWIWATLA-LLLLSHYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHRL 60

Query: 61  SQKYGPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ 120
           SQK+GPIMHM+LGLVNTIVVSSP+AAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ
Sbjct: 61  SQKHGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ 120

Query: 121 YGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLS 180
           YGSYWRNVRKMCTLELLS  KINSFKSMRKEE+GL+I+ +++ ANDG  VDLSS+V SLS
Sbjct: 121 YGSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIECLKKAANDGVAVDLSSKVVSLS 180

Query: 181 TDMTCLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMK 240
           TDMTCLMVFGRKF DEE DDRGFK MIQEVMQLVAAPNLGDF IPF+A FDLQGLTRRMK
Sbjct: 181 TDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDF-IPFIATFDLQGLTRRMK 240

Query: 241 NVSKVFDEFFERIIDEHL--KPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDI 300
           NVS+VFD FFERIIDEHL  K +G KR+KDF+DVMLDL+GSE+TEY++DRSS+KAI+LDI
Sbjct: 241 NVSQVFDGFFERIIDEHLMSKSLGEKRTKDFLDVMLDLIGSEDTEYRVDRSSVKAIILDI 300

Query: 301 LGAAMDTSATLPNDMLP 312
           LGAAMDTSAT+    LP
Sbjct: 301 LGAAMDTSATVIGWALP 315

BLAST of MS025941 vs. NCBI nr
Match: XP_023543390.1 (cytochrome P450 CYP736A12-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 500.0 bits (1286), Expect = 1.7e-137
Identity = 257/317 (81.07%), Postives = 284/317 (89.59%), Query Frame = 0

Query: 1   MPWIWATIALLLLLANYLLQPWLFN----SKKLPPGPKGFPILGSLNLLGKFPHRDLQKL 60
           MPWIWAT+A LLLL++YL Q W FN     KKLPPGPKGFPI+GSLNLL +FPHRDL +L
Sbjct: 1   MPWIWATLA-LLLLSHYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHRL 60

Query: 61  SQKYGPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ 120
           SQK+GPIMHM+LGLVNTIVVSSP+AAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ
Sbjct: 61  SQKHGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ 120

Query: 121 YGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLS 180
           YGSYWRNVRKMCTLELLS  KINSFKSMRKEE+GL+I+ +++ ANDG  VDLSS+V SLS
Sbjct: 121 YGSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIECLKKAANDGVAVDLSSKVVSLS 180

Query: 181 TDMTCLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMK 240
           TDMTCLMVFGRKF DEE DDRGFK MIQEVMQLVAAPNLGDF IPF++ FDLQGLTRRMK
Sbjct: 181 TDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDF-IPFISTFDLQGLTRRMK 240

Query: 241 NVSKVFDEFFERIIDEHL--KPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDI 300
           NVS+VFD FFERIIDEHL  K  G KR+KDF+DVMLDL+GSE+TEY+IDRSS+KAI+LDI
Sbjct: 241 NVSQVFDGFFERIIDEHLKSKSFGEKRTKDFLDVMLDLIGSEDTEYRIDRSSVKAIILDI 300

Query: 301 LGAAMDTSATLPNDMLP 312
           LGAAMDTSAT+    LP
Sbjct: 301 LGAAMDTSATVIGWALP 315

BLAST of MS025941 vs. NCBI nr
Match: XP_023543389.1 (cytochrome P450 CYP736A12-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 500.0 bits (1286), Expect = 1.7e-137
Identity = 257/317 (81.07%), Postives = 284/317 (89.59%), Query Frame = 0

Query: 1   MPWIWATIALLLLLANYLLQPWLFN----SKKLPPGPKGFPILGSLNLLGKFPHRDLQKL 60
           MPWIWAT+A LLLL++YL Q W FN     KKLPPGPKGFPI+GSLNLL +FPHRDL +L
Sbjct: 1   MPWIWATLA-LLLLSHYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHRL 60

Query: 61  SQKYGPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ 120
           SQK+GPIMHM+LGLVNTIVVSSP+AAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ
Sbjct: 61  SQKHGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ 120

Query: 121 YGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLS 180
           YGSYWRNVRKMCTLELLS  KINSFKSMRKEE+GL+I+ +++ ANDG  VDLSS+V SLS
Sbjct: 121 YGSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIECLKKAANDGVAVDLSSKVVSLS 180

Query: 181 TDMTCLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMK 240
           TDMTCLMVFGRKF DEE DDRGFK MIQEVMQLVAAPNLGDF IPF++ FDLQGLTRRMK
Sbjct: 181 TDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDF-IPFISTFDLQGLTRRMK 240

Query: 241 NVSKVFDEFFERIIDEHL--KPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDI 300
           NVS+VFD FFERIIDEHL  K  G KR+KDF+DVMLDL+GSE+TEY+IDRSS+KAI+LDI
Sbjct: 241 NVSQVFDGFFERIIDEHLKSKSFGEKRTKDFLDVMLDLIGSEDTEYRIDRSSVKAIILDI 300

Query: 301 LGAAMDTSATLPNDMLP 312
           LGAAMDTSAT+    LP
Sbjct: 301 LGAAMDTSATVIGWALP 315

BLAST of MS025941 vs. NCBI nr
Match: XP_004143494.1 (cytochrome P450 CYP736A12 [Cucumis sativus])

HSP 1 Score: 496.1 bits (1276), Expect = 2.4e-136
Identity = 252/311 (81.03%), Postives = 280/311 (90.03%), Query Frame = 0

Query: 1   MPWIWAT-IALLLLLANYLLQPWLF----NSKKLPPGPKGFPILGSLNLLGKFPHRDLQK 60
           M WIWAT IALL LL+NY+ + WLF      KKLPPGPKGFPI GSLNLL +FPHRDL +
Sbjct: 1   MAWIWATIIALLFLLSNYIFETWLFKKKSKGKKLPPGPKGFPIFGSLNLLKEFPHRDLHR 60

Query: 61  LSQKYGPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LSQKYGPIMH++LGLVNTI+VSSP AAELFLKTHDL+FASRPLTMVSKFLSYGQKNLVFA
Sbjct: 61  LSQKYGPIMHIKLGLVNTIIVSSPPAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFA 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSL 180
           QYGSYWRNVRKMCTLELLSN KINSFKSMRKEE+GLLI+ ++E ANDG  V++SS+V SL
Sbjct: 121 QYGSYWRNVRKMCTLELLSNHKINSFKSMRKEEVGLLIEYLKEAANDGVSVNISSKVVSL 180

Query: 181 STDMTCLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRM 240
           STDMTCLMVFGRKF DEE DDRGFK MIQEVMQLVAAPNLGD  IPF+AMFDLQGLTRRM
Sbjct: 181 STDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDL-IPFIAMFDLQGLTRRM 240

Query: 241 KNVSKVFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEET-EYQIDRSSIKAIMLDI 300
           KN++KVFD FFERIIDEHLK MG K++KDF+DVMLDLM SE+T EY+IDRSS+KAI+LD+
Sbjct: 241 KNINKVFDRFFERIIDEHLKSMGEKKTKDFLDVMLDLMKSEDTHEYRIDRSSVKAIILDV 300

Query: 301 LGAAMDTSATL 306
           LGAAMDTSAT+
Sbjct: 301 LGAAMDTSATV 310

BLAST of MS025941 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.3e-108
Identity = 197/306 (64.38%), Postives = 253/306 (82.68%), Query Frame = 0

Query: 1   MPWIWATIALLLLLANYLLQPWLFNSKK-LPPGPKGFPILGSLNLLGKFPHRDLQKLSQK 60
           M WIWATI LL L+  ++LQ W  N KK LPPGP+GFPI GSL+LLG+FP++DL +L++K
Sbjct: 1   MVWIWATIGLLALV--HILQAWWKNKKKRLPPGPRGFPIFGSLHLLGEFPNKDLHRLARK 60

Query: 61  YGPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGS 120
           YG IM+MRLGL+ TIV+SSP+AAELFLKTHDLVFASRP    SK +S+GQKNL+F++YG+
Sbjct: 61  YGDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSEYGA 120

Query: 121 YWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKAND-GAVVDLSSEVSSLSTD 180
           YWR+ RKMCT+ELLSN KINSFKSMR+EE+ L ++ +R  AN+ G  VDLS +VSSLS D
Sbjct: 121 YWRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSLSVD 180

Query: 181 MTCLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMKNV 240
           M+C MV G+K+ DEEFD+RGFK++++E +QL +APNLGD+ I F+A  DLQG T+RMK+V
Sbjct: 181 MSCRMVLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDY-IRFIAPLDLQGFTKRMKSV 240

Query: 241 SKVFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDILGAA 300
           +K FD  FE+II+EHL+P   +R+ DFVDVM+  MGSEE+EY+I+R  IKAIMLD+L A+
Sbjct: 241 NKAFDNLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEESEYRIERPHIKAIMLDMLVAS 300

Query: 301 MDTSAT 305
           MDTSAT
Sbjct: 301 MDTSAT 303


HSP 2 Score: 55.5 bits (132), Expect = 1.4e-06
Identity = 24/38 (63.16%), Postives = 29/38 (76.32%), Query Frame = 0

Query: 305 LPNDMLPAQLDMTEEFGLTCPRAQDLIVTPIYRIHSDA 343
           LPN+MLP +LDMTEEFGLT PRA+ L+  P YR+   A
Sbjct: 460 LPNNMLPEELDMTEEFGLTVPRAKHLLAIPSYRLRKSA 497

BLAST of MS025941 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 3.8e-68
Identity = 141/283 (49.82%), Postives = 187/283 (66.08%), Query Frame = 0

Query: 28  KLPPGPKGFPILGSLNLLGKFPHRDLQKLSQKYGPIMHMRLGLVNTIVVSSPQAAELFLK 87
           KLPPGP+G PI+GSL+ LG  PHR LQ L++KYGPIM MRLG V TIVVSSPQAAELFLK
Sbjct: 32  KLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPIMSMRLGSVPTIVVSSPQAAELFLK 91

Query: 88  THDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVRKMCTLELLSNLKINSFKSMRKE 147
           THD +FASRP    ++++SYG   + F  YG +WRN+RK   LELL+  KINSF  MR+E
Sbjct: 92  THDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRNIRKFVVLELLTPAKINSFVGMRRE 151

Query: 148 ELGLLIDRVREKANDGAVVDLSSEVSSLSTDMTCLMVFGRKFSDEEFDDRGFKTMIQEVM 207
           ELG ++  ++E +    VVDLS++V+++  +MT  ++ GR   D+ +D +G   ++ E +
Sbjct: 152 ELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRLLLGRT-KDDRYDLKG---IMNEAL 211

Query: 208 QLVAAPNLGDFNIPFVAMFDLQGLTRRMKNVSKVFDEFFERIIDEH-LKPMGAKRSKDFV 267
            L    N+ DF +PF+   D+QGLTR+ K+  K  D+  E IIDEH         S DF+
Sbjct: 212 TLAGRFNIADF-VPFLGPLDIQGLTRQFKDTGKRLDKILEFIIDEHEQNSSNGNASGDFI 271

Query: 268 DVMLDLMGS-----EETEYQIDRSSIKAIMLDILGAAMDTSAT 305
           D ML L        +E    IDRS IKAIM+DI+ AA+DTS T
Sbjct: 272 DDMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIISAAIDTSDT 309


HSP 2 Score: 44.3 bits (103), Expect = 3.3e-03
Identity = 19/34 (55.88%), Postives = 23/34 (67.65%), Query Frame = 0

Query: 305 LPNDMLPAQLDMTEEFGLTCPRAQDLIVTPIYRI 339
           LPN   P  LDMTE+FGLT PR + L+  P YR+
Sbjct: 467 LPNGTTPDNLDMTEKFGLTTPRVKHLLAVPKYRL 500

BLAST of MS025941 vs. ExPASy Swiss-Prot
Match: Q50EK4 (Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.0e-56
Identity = 118/312 (37.82%), Postives = 193/312 (61.86%), Query Frame = 0

Query: 9   ALLLLLANYLLQPWLFN----SKKLPPGPKGFPILGSLNLLGKFPHRDLQKLSQKYGPIM 68
           A+L+    +    W+ +    +++LPPGP  +PI+G+ + +    HR L+ L++KYGPI+
Sbjct: 18  AILIATFIFFFSCWILHQSQRNERLPPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGPIL 77

Query: 69  HMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNV 128
            +R G V T+VVSS + A+ FLKTHDL+FASRP T V K+  Y  K++ F+ YG +WR +
Sbjct: 78  FLRFGSVPTVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKM 137

Query: 129 RKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAV-VDLSSEVSSLSTDMTCLM 188
           RK+C LELL++ +I SFK +R+EEL  +I  + E++  G + V++S  +S+   ++   +
Sbjct: 138 RKICVLELLTSKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSKAISTSLANILWRI 197

Query: 189 VFGRKFSDEEF--DDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMKNVSKV 248
           +  +KFSD +   D +GF  ++ EV   V + N+GDF IP++   DLQG+ R +K  +  
Sbjct: 198 LARKKFSDNDLGADGKGFADLVVEVSIAVGSLNIGDF-IPYLDCLDLQGIKRALKKANAR 257

Query: 249 FDEFFERIIDEHLKPMGAKRS--------KDFVDVMLDLMGSEETEYQIDRSSIKAIMLD 306
           FD F E++IDEH+     +          KD +DV+L++  ++ T  ++ R  IKAI  +
Sbjct: 258 FDAFAEKMIDEHINASTIRNGEADAGCHVKDIIDVLLEMAKNDNTGAKVTREIIKAITYE 317

BLAST of MS025941 vs. ExPASy Swiss-Prot
Match: A0A068Q721 (Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.1e-54
Identity = 113/297 (38.05%), Postives = 183/297 (61.62%), Query Frame = 0

Query: 9   ALLLLLANYLLQPWLFNSK-KLPPGPKGFPILGSLNLLGKFPHRDLQKLSQKYGPIMHMR 68
           +LL+LL  ++ +      + KLPP P   P++G+L+ LG  PH  L+ L++KYGPI++++
Sbjct: 27  SLLILLLKFIYKDKSRKRRVKLPPSPPKLPVIGNLHQLGNKPHLSLRCLAEKYGPIIYLQ 86

Query: 69  LGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVRKM 128
           LG + T+VVSS + A+  LKTHDL  +SRP    +K L Y   ++VF+ YG+YWR++RK+
Sbjct: 87  LGEIPTVVVSSARLAKEVLKTHDLALSSRPQIFSAKHLFYNCTDVVFSPYGAYWRHIRKI 146

Query: 129 CTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDMTCLMVFGR 188
           C LELLS  ++ SF  +R+EE+  L+ RV E        +L+  +   + D+ C + FGR
Sbjct: 147 CILELLSAKRVQSFSHVREEEVARLVRRVAEFY--PGTTNLTKMLGLYANDVLCRVAFGR 206

Query: 189 KFSD-EEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMKNVSKVFDEFF 248
            FS+  ++D  GF+ M++E  +L+   ++GDF      +  L G+  R++   + FDE F
Sbjct: 207 GFSEGGDYDRHGFQKMLEEYQELLGGFSIGDFFPSMEFIHSLTGMKSRLQETFRRFDELF 266

Query: 249 ERIIDEHLKPMGAKRS-KDFVDVMLDLMGSEETEYQIDRSSIKAIMLDILGAAMDTS 303
           ++++ +HL P   K   KD VDV+LD+   E TE  +   ++KAI+LD+  A  DT+
Sbjct: 267 DQMVTDHLSPKREKEEHKDLVDVLLDIQKKESTEMPLTMDNVKAIILDMFAAGTDTT 321

BLAST of MS025941 vs. ExPASy Swiss-Prot
Match: P37117 (Cytochrome P450 71A4 OS=Solanum melongena OX=4111 GN=CYP71A4 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 5.9e-53
Identity = 123/339 (36.28%), Postives = 198/339 (58.41%), Query Frame = 0

Query: 1   MPWIWATIALLLLLANYLLQPWLFNSKK-----LPPGPKGFPILGSLNLLGKFPHRDLQK 60
           +P +W ++ +LLLL  +LL    F + K     LPP P+  PI+G+L+ LG  PHR L+K
Sbjct: 3   VPCLWYSLLILLLLFIFLLIHHCFTTSKTQNMFLPPSPRKLPIIGNLHQLGSHPHRSLRK 62

Query: 61  LSQKYGPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LSQKYGP+M + LG    IV SS  AA   LKTHD V+A+RP   ++  L YG K++ F+
Sbjct: 63  LSQKYGPVMLLHLGSKPVIVASSVDAARDILKTHDHVWATRPKYSIADSLLYGSKDVGFS 122

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSL 180
            +G YW  VR +  L LLSN ++ S++ +R+EE   +I+++R+   D +V++L   +  L
Sbjct: 123 PFGEYWWQVRSIVVLHLLSNKRVQSYRDVREEETANMIEKIRQGC-DASVINLGEHLCFL 182

Query: 181 STDMTCLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFD-LQGLTRR 240
           + ++T  +  GR + DE       K ++++ +QL+   N+GD+ IP++   + + GL  +
Sbjct: 183 TNNITSRVALGRTY-DERESGIDAKDILEQFLQLLDTFNVGDY-IPWLKWVNKITGLDTK 242

Query: 241 MKNVSKVFDEFFERIIDEHL------KPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIK 300
           ++ ++K  D F + +I+EH+      +      +KDFVDV+L++   +ET++ + R S+K
Sbjct: 243 VEKIAKKLDTFLDSVIEEHIIRNKKEEYAITDEAKDFVDVLLEIQNGKETDFPLQRDSLK 302

Query: 301 AIMLDILGAAMDTSATLPNDMLPAQLDMTEEFGLTCPRA 328
           AI+LD   A  DT  T         LD T    L  PRA
Sbjct: 303 AILLDAFAAGTDTIYT--------NLDWTMADVLRQPRA 330

BLAST of MS025941 vs. ExPASy TrEMBL
Match: A0A6J1BWX1 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005474 PE=3 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 6.5e-172
Identity = 341/501 (68.06%), Postives = 341/501 (68.06%), Query Frame = 0

Query: 1   MPWIWATIALLLLLANYLLQPWLFNSKKLPPGPKGFPILGSLNLLGKFPHRDLQKLSQKY 60
           MPWIWATIALLLLLANYLLQPWLFNSKKLPPGPKGFPILG LNLLGKFPHRDLQKLSQKY
Sbjct: 1   MPWIWATIALLLLLANYLLQPWLFNSKKLPPGPKGFPILGCLNLLGKFPHRDLQKLSQKY 60

Query: 61  GPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSY 120
           GPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSY
Sbjct: 61  GPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSY 120

Query: 121 WRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDMT 180
           WRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDMT
Sbjct: 121 WRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDMT 180

Query: 181 CLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMKNVSK 240
           CLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVA FDLQGLTRRMKNVSK
Sbjct: 181 CLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVATFDLQGLTRRMKNVSK 240

Query: 241 VFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDILGAAMD 300
           VFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDILGAAMD
Sbjct: 241 VFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDILGAAMD 300

Query: 301 TSAT-------------------------------------------------------- 346
           TSAT                                                        
Sbjct: 301 TSATVIGWALPELIKNPQVMKKVQDELEKVVGLDRMVEESDLVNLEYLDMVIKETLRLYP 360

BLAST of MS025941 vs. ExPASy TrEMBL
Match: A0A6J1GFR1 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453773 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.8e-138
Identity = 257/317 (81.07%), Postives = 285/317 (89.91%), Query Frame = 0

Query: 1   MPWIWATIALLLLLANYLLQPWLFN----SKKLPPGPKGFPILGSLNLLGKFPHRDLQKL 60
           MPWIWAT+A LLLL++YL Q W FN     KKLPPGPKGFPI+GSLNLL +FPHRDL +L
Sbjct: 1   MPWIWATLA-LLLLSHYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHRL 60

Query: 61  SQKYGPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ 120
           SQK+GPIMHM+LGLVNTIVVSSP+AAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ
Sbjct: 61  SQKHGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ 120

Query: 121 YGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLS 180
           YGSYWRNVRKMCTLELLS  KINSFKSMRKEE+GL+I+ +++ ANDG  VDLSS+V SLS
Sbjct: 121 YGSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIECLKKAANDGVAVDLSSKVVSLS 180

Query: 181 TDMTCLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMK 240
           TDMTCLMVFGRKF DEE DDRGFK MIQEVMQLVAAPNLGDF IPF+A FDLQGLTRRMK
Sbjct: 181 TDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDF-IPFIATFDLQGLTRRMK 240

Query: 241 NVSKVFDEFFERIIDEHL--KPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDI 300
           NVS+VFD FFERIIDEHL  K +G KR+KDF+DVMLDL+GSE+TEY++DRSS+KAI+LDI
Sbjct: 241 NVSQVFDGFFERIIDEHLMSKSLGEKRTKDFLDVMLDLIGSEDTEYRVDRSSVKAIILDI 300

Query: 301 LGAAMDTSATLPNDMLP 312
           LGAAMDTSAT+    LP
Sbjct: 301 LGAAMDTSATVIGWALP 315

BLAST of MS025941 vs. ExPASy TrEMBL
Match: A0A6J1IMS2 (cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478444 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 8.4e-135
Identity = 252/317 (79.50%), Postives = 280/317 (88.33%), Query Frame = 0

Query: 1   MPWIWATIALLLLLANYLLQPWLFN----SKKLPPGPKGFPILGSLNLLGKFPHRDLQKL 60
           M WIWAT+A LLLL++YL Q W FN     KKLPPGPKGFPI+GSLNLL +FPHRDL +L
Sbjct: 1   MAWIWATLA-LLLLSHYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHRL 60

Query: 61  SQKYGPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ 120
           SQKYGPIMHM+LGLVNT VVSS +AAELFLKTHDLVFASRPLTMVSKFLSYGQKNL+FAQ
Sbjct: 61  SQKYGPIMHMKLGLVNTTVVSSAEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLIFAQ 120

Query: 121 YGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLS 180
           YGSYWRNVRKMCTLELLS  KINSFKSMRKEE+GL+I+ +++ ANDG  VDLSS++ SLS
Sbjct: 121 YGSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIEYLKKAANDGVAVDLSSKIVSLS 180

Query: 181 TDMTCLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMK 240
           TDMTCLMVFGRKF DEE DDRGFK MIQEVMQLVAAPNLGDF IPF+A FDLQGLTRRMK
Sbjct: 181 TDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDF-IPFIATFDLQGLTRRMK 240

Query: 241 NVSKVFDEFFERIIDEHLK--PMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDI 300
            VS+VFD FFERIIDEHLK   +G KR+KDF+D MLDL+GSE+TEY+IDRSS+KAI+LDI
Sbjct: 241 IVSQVFDGFFERIIDEHLKSRSLGEKRTKDFLDFMLDLIGSEDTEYRIDRSSVKAIILDI 300

Query: 301 LGAAMDTSATLPNDMLP 312
           LGAAMDTSAT+    LP
Sbjct: 301 LGAAMDTSATVIGWALP 315

BLAST of MS025941 vs. ExPASy TrEMBL
Match: A0A6J1IMS2 (cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478444 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 4.3e-06
Identity = 28/35 (80.00%), Postives = 30/35 (85.71%), Query Frame = 0

Query: 305 LPNDMLPAQLDMTEEFGLTCPRAQDLIVTPIYRIH 340
           L NDM   +LDMTEEFGLTCPRAQDLIV PIYR+H
Sbjct: 465 LSNDMSVDELDMTEEFGLTCPRAQDLIVVPIYRLH 499


HSP 2 Score: 427.9 bits (1099), Expect = 3.9e-116
Identity = 214/305 (70.16%), Postives = 260/305 (85.25%), Query Frame = 0

Query: 1   MPWIWATIALLLLLANYLLQPWLFNSK-KLPPGPKGFPILGSLNLLGKFPHRDLQKLSQK 60
           M WI   +A L  LA  +L+PWLF S+ KLPPGP+GFP+ GSL+LLGK PHRDL+ LSQK
Sbjct: 1   MAWILLAVA-LAGLALLILKPWLFRSQNKLPPGPRGFPVFGSLHLLGKLPHRDLRDLSQK 60

Query: 61  YGPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGS 120
           YG IM+MRLG+V TI+V+SPQAAELFLKTHDLVFASRP    SK++SYGQKNL FAQYGS
Sbjct: 61  YGSIMYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQYGS 120

Query: 121 YWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDM 180
           YWRN+RKMCTLELLS+ K+NSF+SMR+EE+GLL+  +R+ A D  V++LSS+++SL+TDM
Sbjct: 121 YWRNMRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLNTDM 180

Query: 181 TCLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMKNVS 240
           TCLMVFG+K++D EFD+RGFK++IQE MQLVA PNLGDF IP +A  DLQGL RR K +S
Sbjct: 181 TCLMVFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDF-IPCIAALDLQGLNRRSKAIS 240

Query: 241 KVFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDILGAAM 300
           K+FD FFE+IIDEHL+     ++KDFVDVMLD+MGS+ETEYQIDRSSIKAI+LD+L AAM
Sbjct: 241 KIFDGFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVAAM 300

Query: 301 DTSAT 305
           DTSAT
Sbjct: 301 DTSAT 303

BLAST of MS025941 vs. ExPASy TrEMBL
Match: A0A6J1BYU0 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 3.5e-08
Identity = 30/36 (83.33%), Postives = 34/36 (94.44%), Query Frame = 0

Query: 305 LPNDMLPAQLDMTEEFGLTCPRAQDLIVTPIYRIHS 341
           LPN MLPA+LDMTEEFGLTCPRAQDL+VTPIYR+ +
Sbjct: 460 LPNGMLPAELDMTEEFGLTCPRAQDLMVTPIYRLQN 495


HSP 2 Score: 422.2 bits (1084), Expect = 2.1e-114
Identity = 213/305 (69.84%), Postives = 252/305 (82.62%), Query Frame = 0

Query: 1   MPWIWATIALLLLLANYLLQPWLFNSKKLPPGPKGFPILGSLNLLGKFPHRDLQKLSQKY 60
           M WIWA   LLL LA  L +PWLF  K LPPGPKGF I GSL+LLGK PHRDLQKLSQKY
Sbjct: 1   MAWIWAISPLLLALA-LLFKPWLFKPKNLPPGPKGFSIFGSLHLLGKLPHRDLQKLSQKY 60

Query: 61  GPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSY 120
           GPIMHM+LGLV TI+VSSP AAELFLKTHDLVFASRPL+  SK ++Y QKNLVFA YGSY
Sbjct: 61  GPIMHMKLGLVPTIIVSSPSAAELFLKTHDLVFASRPLSEASKQMNYCQKNLVFAPYGSY 120

Query: 121 WRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDMT 180
           WRN+RKMCTLELLS+ KINSFKSMRK EL LLID +RE A++G  V+LSS+V+SL+TDMT
Sbjct: 121 WRNMRKMCTLELLSSRKINSFKSMRKRELALLIDHLREAADNGVAVNLSSKVTSLTTDMT 180

Query: 181 CLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMKNVSK 240
           CLMVFG K+ D++FD+RGFK ++QE  QL A PNLGDF  PF+A  DLQ L ++MK+V K
Sbjct: 181 CLMVFGMKYEDKDFDERGFKAVVQEGSQLAATPNLGDF-FPFIAGLDLQRLNKQMKSVHK 240

Query: 241 VFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDILGAAMD 300
             D+F ERI+++HL+    +++KDFVDVMLD+M  +ETEYQIDR +IKAIMLD+L AAMD
Sbjct: 241 ALDDFLERIVNDHLESKDEEKTKDFVDVMLDVMSFQETEYQIDRPAIKAIMLDMLTAAMD 300

Query: 301 TSATL 306
           TSAT+
Sbjct: 301 TSATV 303

BLAST of MS025941 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 203.8 bits (517), Expect = 2.3e-52
Identity = 113/303 (37.29%), Postives = 185/303 (61.06%), Query Frame = 0

Query: 8   IALLLLLANYLLQPWLFNSKKLPPGPKGFPILGSLNLLGKFPHRDLQKLSQKYGPIMHMR 67
           ++LL ++++  L+    +   LPP P   PI+G+L+ L   PHR   KLS KYGP++ +R
Sbjct: 10  VSLLTIVSSIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYGPLVFLR 69

Query: 68  LGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVRKM 127
           LG V  +V+SS +AAE  LKT+DL   SRP T+ S  LSYG K++ FA YG YWR VRK+
Sbjct: 70  LGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWREVRKL 129

Query: 128 CTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDMTCLMVFGR 187
             +EL S+ K+ SF+ +R+EE+  ++ +V E A   + VDLS    SL+  + C +  G+
Sbjct: 130 AVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIICRVALGQ 189

Query: 188 KFSDEEF--DDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFDLQGLTRRMKNVSKVF--- 247
            F++  F  D    + ++ E  + +      DF    +  F +  L +R K ++KVF   
Sbjct: 190 NFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRF-VDWLFQRHKKINKVFKEL 249

Query: 248 DEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEE--TEYQIDRSSIKAIMLDILGAAMD 304
           D F++ +ID+HLKP G ++++D V ++LD++  +E    ++++  ++KAI++D+  A +D
Sbjct: 250 DAFYQHVIDDHLKPEG-RKNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVMDVFLAGID 309

BLAST of MS025941 vs. TAIR 10
Match: AT5G24960.1 (cytochrome P450, family 71, subfamily A, polypeptide 14 )

HSP 1 Score: 196.8 bits (499), Expect = 2.8e-50
Identity = 112/301 (37.21%), Postives = 181/301 (60.13%), Query Frame = 0

Query: 6   ATIALLLLLANYLLQPWLFNSKKLPPGPKGFPILGSLNLLGKFPHRDLQKLSQKYGPIMH 65
           ATI  LLLL  +L + +      LPP P   P++G+L+ L   PHR L+ LS +YGP+M 
Sbjct: 11  ATILALLLLKQFLNRTYT-AKVNLPPSPWRVPVIGNLHQLSLHPHRSLRSLSHRYGPLML 70

Query: 66  MRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVR 125
           +  G V  +VVSS   A   +KTHDL  A+RP   V + +  G + +VF+ YG YWR ++
Sbjct: 71  LHFGRVPVLVVSSSDVAHDLMKTHDLKVANRPQLKVVEKIFNGGREMVFSPYGEYWRQIK 130

Query: 126 KMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDMTCLMVF 185
            +C + LL+  K+ SF+ +R+EE+  +++RV + ++D + ++LS  + +L++D+T  +  
Sbjct: 131 SVCIVNLLNKKKVQSFEKVREEEISEMMERVEKASSDSSPLNLSELLLTLTSDVTSRVSL 190

Query: 186 GRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMFD-LQGLTRRMKNVSKVFDE 245
           GRK+S EE     FK  ++++ +LV    +G++ IP +A  D L+G+  + + VSK F +
Sbjct: 191 GRKYSKEE-SMSDFKIQMRKITELVGGFPVGEY-IPCLAWIDKLRGVDEKAEEVSKAFGD 250

Query: 246 FFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDILGAAMDTSAT 305
             E+++ EHL     K + DFVDV+L L   E    QI RS IK ++LD+  A  +T+  
Sbjct: 251 LMEKVLQEHLDATD-KPTLDFVDVLLSLERHERNGVQIRRSDIKFLILDMFLAGTETTYA 307

BLAST of MS025941 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 195.3 bits (495), Expect = 8.2e-50
Identity = 101/276 (36.59%), Postives = 173/276 (62.68%), Query Frame = 0

Query: 31  PGPKGFPILGSLNLLGKFPHRDLQKLSQKYGPIMHMRLGLVNTIVVSSPQAAELFLKTHD 90
           P P G P++G+L+ LG+ PHR L  LS +YGP+M +  G V  +VVSS + A   LKTHD
Sbjct: 33  PSPPGLPLIGNLHQLGRHPHRSLCSLSHRYGPLMLLHFGRVPVLVVSSAELARDVLKTHD 92

Query: 91  LVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELG 150
            VFASRP + + + L Y + ++  A YG YWR ++ +C L L SN  + SF+ +R+EE+ 
Sbjct: 93  RVFASRPRSKIFEKLLYDKHDVASAPYGEYWRQMKSVCVLHLFSNKMVRSFREVREEEIS 152

Query: 151 LLIDRVREKANDGAVVDLSSEVSSLSTDMTCLMVFGRKFSDEEFDDRGFKTMIQEVMQLV 210
           L+++++R+  +    V+LS  + SL+ D+ C +  GRK+  E      FK +++ + +L+
Sbjct: 153 LMMEKIRKSIS--LPVNLSKILVSLTNDVICKVALGRKYGGE----TDFKELMERLNKLL 212

Query: 211 AAPNLGDFNIPFVAMFD-LQGLTRRMKNVSKVFDEFFERIIDEHLKPMGAKRSKDFVDVM 270
              ++G + +P++A  D ++GL  +++  +   D+FFER++ +H+   G +   DFVDV+
Sbjct: 213 GTFSVGSY-VPWLAWIDWIRGLDCQLEKTANDVDKFFERVVQDHVD--GNRDMTDFVDVL 272

Query: 271 LDLMGSEETEYQIDRSSIKAIMLDILGAAMDTSATL 306
           L +   +   ++I+R SIKAI++++     DTS+TL
Sbjct: 273 LAIQRDKTVGFEINRVSIKAIVMNVFVGGTDTSSTL 299

BLAST of MS025941 vs. TAIR 10
Match: AT5G42590.1 (cytochrome P450, family 71, subfamily A, polypeptide 16 )

HSP 1 Score: 193.7 bits (491), Expect = 2.4e-49
Identity = 105/300 (35.00%), Postives = 179/300 (59.67%), Query Frame = 0

Query: 8   IALLLLLANYLLQPWLFNSKKLPPGPKGFPILGSLNLLGKFPHRDLQKLSQKYGPIMHMR 67
           + +LL   + L +P    +  LPP P   P++G+L+ L   PHR L  LS ++GP+M +R
Sbjct: 15  LTILLFFKSLLKRP----NSNLPPSPWRLPVIGNLHQLSLHPHRALSSLSARHGPLMLLR 74

Query: 68  LGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVRKM 127
            G V  ++VSS   A   +KTHDL FA+RP+T  +  +S G ++LVFA YG YWRNV+ +
Sbjct: 75  FGRVPVLIVSSADVAHDVMKTHDLKFANRPITKSAHKISNGGRDLVFAPYGEYWRNVKSL 134

Query: 128 CTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLSTDMTCLMVFGR 187
           CT+ LLSN  + S +  R+EE+ LL++ + E +   + V+LS  ++++ +D+   +V G+
Sbjct: 135 CTIHLLSNKMVQSSEKRREEEITLLMETLEEASLSSSSVNLSKLITNMVSDIMGKVVLGK 194

Query: 188 KFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVAMF-DLQGLTRRMKNVSKVFDEFF 247
           K+S EE      KT+ +  +  V    +G++ IP +A    + G   +++ ++K F +F 
Sbjct: 195 KYSGEE-GTIDVKTITKSFLDAVGLSPVGEY-IPSLAWIGKITGSDGKLEKITKQFGDFI 254

Query: 248 ERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDI-LGAAMDTSATL 306
           E+++ EH      K + DFVD++L +   E  + Q+D+S +K I+ ++ LG+   TSA +
Sbjct: 255 EKVLQEHEDTTADKETPDFVDMLLTIQRDETAQCQLDKSDLKVIIFEMFLGSTTTTSAVI 308

BLAST of MS025941 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 193.0 bits (489), Expect = 4.1e-49
Identity = 102/277 (36.82%), Postives = 171/277 (61.73%), Query Frame = 0

Query: 30  PPGPKGFPILGSLNLLGKFPHRDLQKLSQKYGPIMHMRLGLVNTIVVSSPQAAELFLKTH 89
           P  P   P++G+L+ LG+ PHR L  LS +YGP+M +R GLV  +VVSS   A   LKT+
Sbjct: 33  PASPPRLPLIGNLHQLGRHPHRSLCSLSNRYGPLMLLRFGLVPVLVVSSADVARDILKTY 92

Query: 90  DLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVRKMCTLELLSNLKINSFKSMRKEEL 149
           D VFASRP + + + + Y  +++  A YG YWR ++ +C L LL+N  + SF+++R+EE+
Sbjct: 93  DRVFASRPRSKIFEKIFYEARDVALAPYGEYWRQMKSVCVLHLLTNKMVRSFRNVRQEEI 152

Query: 150 GLLIDRVREKANDGAVVDLSSEVSSLSTDMTCLMVFGRKFSDEEFDDRGFKTMIQEVMQL 209
            L+++++++ ++    V+LS  + SL+ D+   +  GRK+SDE      FK +++ + +L
Sbjct: 153 SLMMEKIQKSSS--LQVNLSELLGSLTNDVISRVALGRKYSDE----TDFKELMKRLTKL 212

Query: 210 VAAPNLGDFNIPFVAMFD-LQGLTRRMKNVSKVFDEFFERIIDEHLKPMGAKRSKDFVDV 269
           +    +G + +P++A  D + GL  ++K      DEF E+++ +H    G  +  DFVDV
Sbjct: 213 LGEFCVGTY-VPWLAWIDWISGLDGQLKKTGNDLDEFLEKVVQDH--EDGDAQRTDFVDV 272

Query: 270 MLDLMGSEETEYQIDRSSIKAIMLDILGAAMDTSATL 306
           +L +   +   ++IDR SIKAI+LD++    DTS  L
Sbjct: 273 LLRIQREKSVGFEIDRLSIKAIILDVVVGGTDTSYAL 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132668.11.4e-17168.06cytochrome P450 CYP736A12-like [Momordica charantia][more]
XP_022950766.15.7e-13881.07cytochrome P450 CYP736A12-like [Cucurbita moschata][more]
XP_023543390.11.7e-13781.07cytochrome P450 CYP736A12-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023543389.11.7e-13781.07cytochrome P450 CYP736A12-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_004143494.12.4e-13681.03cytochrome P450 CYP736A12 [Cucumis sativus][more]

Pages

Match NameE-valueIdentityDescription
A0A068Q5V61.3e-10864.38Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1[more]
H2DH183.8e-6849.82Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
Q50EK42.0e-5637.82Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1[more]
A0A068Q7213.1e-5438.05Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1[more]
P371175.9e-5336.28Cytochrome P450 71A4 OS=Solanum melongena OX=4111 GN=CYP71A4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BWX16.5e-17268.06cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005474 PE... [more]
A0A6J1GFR12.8e-13881.07cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453773 PE=... [more]
A0A6J1IMS28.4e-13579.50cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478444 PE=3 ... [more]
A0A6J1IMS24.3e-0680.00cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478444 PE=3 ... [more]
A0A6J1BYU03.5e-0883.33cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE... [more]

Pages

Match NameE-valueIdentityDescription
AT1G13080.12.3e-5237.29cytochrome P450, family 71, subfamily B, polypeptide 2 [more]
AT5G24960.12.8e-5037.21cytochrome P450, family 71, subfamily A, polypeptide 14 [more]
AT3G48270.18.2e-5036.59cytochrome P450, family 71, subfamily A, polypeptide 26 [more]
AT5G42590.12.4e-4935.00cytochrome P450, family 71, subfamily A, polypeptide 16 [more]
AT3G48310.14.1e-4936.82cytochrome P450, family 71, subfamily A, polypeptide 22 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 57..76
score: 32.5
coord: 175..193
score: 20.18
coord: 286..303
score: 27.53
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..306
e-value: 9.6E-59
score: 201.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 30..305
IPR001128Cytochrome P450PFAMPF00067p450coord: 30..304
e-value: 1.4E-41
score: 142.7
NoneNo IPR availablePANTHERPTHR47943CYTOCHROME P450 93A3-LIKEcoord: 305..339
coord: 1..304
NoneNo IPR availablePANTHERPTHR47943:SF2CYTOCHROME P450 FAMILY 71 PROTEINcoord: 305..339
coord: 1..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS025941.1MS025941.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen