MS025919 (gene) Bitter gourd (TR) v1

Overview


Name	MS025919
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic
Location	scaffold391: 1094890 .. 1096228 (+)
RNA-Seq Expression	MS025919
Synteny	MS025919

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAGCGAGGGAAGAAGGAAGATAACGAGCTTGCACATGGTAAGCGCTATTAGAGATTGGGGATTGCAAAATTTGGTGGAGGATGTTATTAGTTTGGCTGGGCCAAGAGGAAATGCGTGGGCAATTGGAGCACAAAATGCTGGGAAAAGCACGTTGATTAATTCAATAGGAATGCACGTTGGTGGGAAGATCACGCAATTGACTGAAGCACCAGTGCCTGGAACAACTTTGGGGATAATCAGAGTGGAGGGCATTCGTCCGGGTCAAGCCAAGTTGTTCGATACGCCTGGGCTTTTGAATCCCCACCAGATTACGACAAGATTGACAAGGGAAGAGCAGAAACTTGTTCACATTAGCAAGGAATTGAAGCCGAGGACTTACAGGATAAAGGTAAATCTAAATCAGATTTAGATGCTGGTTAGCTATATATTCCTTGAACTATAAATTCATGATCTTTGAAATGTATTATATGGATTGAAGTGAAATTTTTATTGGCTAATATGCAAAATAGCATATACATTAATTTTAACTTCAAGCATAAGTTGAGATCATTAGTATTAAGTAATGTGCTAGAGATTTCATTCTTTTTGTCCGTTTTTTAGGCTGGTCATACAATTCATGTTGCGGGTGTCATGAGGCTAGATGTGGAAGAAACATTTGTAGATTCAATTTATGTTACAGTGTGGGCTCTCCTTATCTACTATTACACATGGGGAAAATAGAAAATGCAAGCAAGATACAAGAGGATGATTTTGGCAATCAGTTACAGTATTATTTTCACTATAACCATTTGCATCATGTTGCTGAAATTTGAGTGCTGTCCCAGAATTGGTTTATATAAGCGTCTTCCGAGTTTCATTGTCAATGGCTTCCTAAATGACATAGTCTTAAGAATGTGCATATGGCTTCTATTATTTATCTCATATCGATATTGTTTGATACTTATGATCCTGTTTTTTATTATTCGATACTTATGATCATGTTATGAATAGTTAATCGGTGGTTTAACTGCCCTTCTCGTTTTTCATTTTGCAACATTTGGGGTGCCATAGTGTTTTCATATTCTTTTTTGGAAAGCCTTGATCTCATATTTGTGGAAAGTTGGCTTCGGAATTTCCTTACTCTCTTACATTTATGTAGTTGTTTAGGGTTTCTGTTATAGTAAATGTAATTGTTAAGGAATCCTCAATGTTTCATGCTTCAGCCACCTATAGGTAAAGATCGAGTTGCAGAGCTTGGAAAATGGGTGAGAAGAGAATTTCGTGTTTGTGGTAGTAGTTGGGATTCAAGTTGGGTAGACGTTGCTGTTGCTGGCCTCGGGTGGTTTGCTATTTGA

mRNA sequence

ATGGAGAGCGAGGGAAGAAGGAAGATAACGAGCTTGCACATGGTAAGCGCTATTAGAGATTGGGGATTGCAAAATTTGGTGGAGGATGTTATTAGTTTGGCTGGGCCAAGAGGAAATGCGTGGGCAATTGGAGCACAAAATGCTGGGAAAAGCACGTTGATTAATTCAATAGGAATGCACGTTGGTGGGAAGATCACGCAATTGACTGAAGCACCAGTGCCTGGAACAACTTTGGGGATAATCAGAGTGGAGGGCATTCGTCCGGGTCAAGCCAAGTTGTTCGATACGCCTGGGCTTTTGAATCCCCACCAGATTACGACAAGATTGACAAGGGAAGAGCAGAAACTTGTTCACATTAGCAAGGAATTGAAGCCGAGGACTTACAGGATAAAGCCACCTATAGGTAAAGATCGAGTTGCAGAGCTTGGAAAATGGGTGAGAAGAGAATTTCGTGTTTGTGGTAGTAGTTGGGATTCAAGTTGGGTAGACGTTGCTGTTGCTGGCCTCGGGTGGTTTGCTATTTGA

Coding sequence (CDS)

ATGGAGAGCGAGGGAAGAAGGAAGATAACGAGCTTGCACATGGTAAGCGCTATTAGAGATTGGGGATTGCAAAATTTGGTGGAGGATGTTATTAGTTTGGCTGGGCCAAGAGGAAATGCGTGGGCAATTGGAGCACAAAATGCTGGGAAAAGCACGTTGATTAATTCAATAGGAATGCACGTTGGTGGGAAGATCACGCAATTGACTGAAGCACCAGTGCCTGGAACAACTTTGGGGATAATCAGAGTGGAGGGCATTCGTCCGGGTCAAGCCAAGTTGTTCGATACGCCTGGGCTTTTGAATCCCCACCAGATTACGACAAGATTGACAAGGGAAGAGCAGAAACTTGTTCACATTAGCAAGGAATTGAAGCCGAGGACTTACAGGATAAAGCCACCTATAGGTAAAGATCGAGTTGCAGAGCTTGGAAAATGGGTGAGAAGAGAATTTCGTGTTTGTGGTAGTAGTTGGGATTCAAGTTGGGTAGACGTTGCTGTTGCTGGCCTCGGGTGGTTTGCTATTTGA

Protein sequence

MESEGRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGKITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKPPIGKDRVAELGKWVRREFRVCGSSWDSSWVDVAVAGLGWFAI

Homology

[Close]

BLAST of MS025919 vs. NCBI nr
Match: XP_022150848.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 295.0 bits (754), Expect = 4.1e-76
Identity = 156/226 (69.03%), Postives = 160/226 (70.80%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  KITSLHMVSA+RDWGL+NLVEDV+SL GPRGN WAIGAQNAGKSTLINSIGMHVGGK
Sbjct: 186 GMNKITSLHMVSAVRDWGLKNLVEDVVSLVGPRGNVWAIGAQNAGKSTLINSIGMHVGGK 245

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           ITQLTEA VPGTTLGIIRVEGI PGQAKLFDTPGLLNPHQITTRLTREEQKLVHI+KELK
Sbjct: 246 ITQLTEAAVPGTTLGIIRVEGILPGQAKLFDTPGLLNPHQITTRLTREEQKLVHINKELK 305

Query: 125 PRTYRIK----------------------------------------------------- 175
           PRTYRIK                                                     
Sbjct: 306 PRTYRIKAGHTVHVAGLMRLDVEETTVDSIYVTVWASPYLPLHMGKTENASKIQEDHFGN 365

[Close]

BLAST of MS025919 vs. NCBI nr
Match: XP_022150846.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform X1 [Momordica charantia] >XP_022150847.1 GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 295.0 bits (754), Expect = 4.1e-76
Identity = 156/226 (69.03%), Postives = 160/226 (70.80%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  KITSLHMVSA+RDWGL+NLVEDV+SL GPRGN WAIGAQNAGKSTLINSIGMHVGGK
Sbjct: 280 GMNKITSLHMVSAVRDWGLKNLVEDVVSLVGPRGNVWAIGAQNAGKSTLINSIGMHVGGK 339

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           ITQLTEA VPGTTLGIIRVEGI PGQAKLFDTPGLLNPHQITTRLTREEQKLVHI+KELK
Sbjct: 340 ITQLTEAAVPGTTLGIIRVEGILPGQAKLFDTPGLLNPHQITTRLTREEQKLVHINKELK 399

Query: 125 PRTYRIK----------------------------------------------------- 175
           PRTYRIK                                                     
Sbjct: 400 PRTYRIKAGHTVHVAGLMRLDVEETTVDSIYVTVWASPYLPLHMGKTENASKIQEDHFGN 459

[Close]

BLAST of MS025919 vs. NCBI nr
Match: XP_038901740.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 293.1 bits (749), Expect = 1.6e-75
Identity = 155/225 (68.89%), Postives = 159/225 (70.67%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  KITSLHMVSA+RDWGL+NLVEDVI L G RGN WAIGAQNAGKSTLINSIG HVGGK
Sbjct: 273 GINKITSLHMVSAVRDWGLKNLVEDVIDLVGARGNVWAIGAQNAGKSTLINSIGKHVGGK 332

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           ITQLTEAPVPGTTLGIIR+EGI PGQA+LFDTPGLLNPHQITTRLTREEQKLVHISKELK
Sbjct: 333 ITQLTEAPVPGTTLGIIRMEGIFPGQARLFDTPGLLNPHQITTRLTREEQKLVHISKELK 392

Query: 125 PRTYRIK----------------------------------------------------- 175
           PRTYRIK                                                     
Sbjct: 393 PRTYRIKVGHTIHVAGLMRLDVEETSVDTIYVTVWASPYLPLHMGKTENASKIQDHFGNQ 452

[Close]

BLAST of MS025919 vs. NCBI nr
Match: XP_023513264.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 292.7 bits (748), Expect = 2.0e-75
Identity = 154/226 (68.14%), Postives = 159/226 (70.35%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  KITSLHMVSA+RDWGL++LVEDVI L GPRGN WA+GAQNAGKSTLINSIG HVGGK
Sbjct: 282 GINKITSLHMVSAVRDWGLKSLVEDVIDLVGPRGNVWAVGAQNAGKSTLINSIGKHVGGK 341

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           ITQLTEAPVPGTTLGIIRVEG+ PGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK
Sbjct: 342 ITQLTEAPVPGTTLGIIRVEGMFPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 401

Query: 125 PRTYRIK----------------------------------------------------- 175
           PRTYRIK                                                     
Sbjct: 402 PRTYRIKAGHTIHVAGLMRLDVEETSVDTIYVTVWASPYLPLHMGKTENASKILEDHFGN 461

[Close]

BLAST of MS025919 vs. NCBI nr
Match: KAG6570359.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010240.1 GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 292.0 bits (746), Expect = 3.5e-75
Identity = 154/226 (68.14%), Postives = 159/226 (70.35%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  KITSLHMVSAIRDWGL++LVEDVI L GPRGN WA+GAQNAGKSTLINSIG HVGGK
Sbjct: 282 GINKITSLHMVSAIRDWGLKSLVEDVIDLVGPRGNVWAVGAQNAGKSTLINSIGKHVGGK 341

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           ITQLTEAPVPGTTLGIIRVEG+ PGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK
Sbjct: 342 ITQLTEAPVPGTTLGIIRVEGMFPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 401

Query: 125 PRTYRIK----------------------------------------------------- 175
           PRTYRIK                                                     
Sbjct: 402 PRTYRIKAGHTIHVAGLMRLDLEETSVDTIYVTVWASPYLPLHMGKTENASKILEDHFGN 461

[Close]

BLAST of MS025919 vs. ExPASy Swiss-Prot
Match: Q8W4I6 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BPG2 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.0e-45
Identity = 94/226 (41.59%), Postives = 134/226 (59.29%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  K++ ++MVSA +D G++NL+  +  LAGPRGN W IGAQNAGKSTLIN++    G K
Sbjct: 360 GAPKLSGVYMVSARKDIGVKNLLAYIKELAGPRGNVWVIGAQNAGKSTLINALSKKDGAK 419

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           +T+LTEAPVPGTTLGI+++ GI   +AK++DTPGLL+P+ ++ RL  EE+K+V I KE++
Sbjct: 420 VTRLTEAPVPGTTLGILKIGGILSAKAKMYDTPGLLHPYLMSLRLNSEERKMVEIRKEVQ 479

Query: 125 PRTYRIK----------------------------------------------------- 175
           PR+YR+K                                                     
Sbjct: 480 PRSYRVKAGQSVHIGGLVRLDLVSASVETIYITIWASHSVSLHLGKTENAEEIFKGHSGL 539

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BLAST of MS025919 vs. ExPASy Swiss-Prot
Match: P54453 (Uncharacterized protein YqeH OS=Bacillus subtilis (strain 168) OX=224308 GN=yqeH PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.5e-12
Identity = 42/123 (34.15%), Postives = 69/123 (56.10%), Query Frame = 0


Query: 8   KITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGKITQ 67
           K   + +VSA R  G++ ++ D I       + + +G  N GKST IN I   V G+   
Sbjct: 131 KPVDVFLVSAGRGQGIREVI-DAIEHYRNGKDVYVVGCTNVGKSTFINRIIKEVSGEEDI 190

Query: 68  LTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRT 127
           +T +  PGTTL  I +       + L+DTPG++N HQ+   + +++ K++   KELKPRT
Sbjct: 191 ITTSQFPGTTLDAIEIP--LDDGSSLYDTPGIINNHQMAHYVNKKDLKILSPKKELKPRT 250

Query: 128 YRI 131
           +++
Sbjct: 251 FQL 250

[Close]

BLAST of MS025919 vs. ExPASy Swiss-Prot
Match: Q8NC60 (Nitric oxide-associated protein 1 OS=Homo sapiens OX=9606 GN=NOA1 PE=1 SV=2)

HSP 1 Score: 47.8 bits (112), Expect = 1.5e-04
Identity = 33/125 (26.40%), Postives = 61/125 (48.80%), Query Frame = 0


Query: 6   RRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSI------GM 65
           R  +  + ++SA   +G++ L+  +      RG+ + +GA NAGKSTL N++        
Sbjct: 307 RTVVRDVRLISAKTGYGVEELISALQRSWRYRGDVYLVGATNAGKSTLFNTLLESDYCTA 366

Query: 66  HVGGKITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLL--NPHQITTRLTREEQKLV 123
                I + T +P PGTTL +++     P   ++F     L  +  Q    L+ +EQ  +
Sbjct: 367 KGSEAIDRATISPWPGTTLNLLKFPICNPTPYRMFKRHQRLKKDSTQAEEDLSEQEQNQL 426

[Close]

BLAST of MS025919 vs. ExPASy Swiss-Prot
Match: Q32LB9 (Nitric oxide-associated protein 1 OS=Bos taurus OX=9913 GN=NOA1 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 4.4e-04
Identity = 32/130 (24.62%), Postives = 67/130 (51.54%), Query Frame = 0


Query: 1   MESEGRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSI--- 60
           + S+    +  + ++SA   +G++ L+  +      RG+ + +G+ NAGKSTL N++   
Sbjct: 303 LPSKSSTVLRDVRLISAKTGYGVEELISALQRSWRYRGDVYLVGSTNAGKSTLFNTLLES 362

Query: 61  --GMHVGGK-ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLL--NPHQITTRLTRE 120
              +  G + I + T +P PGTTL +++     P   ++F     L  +  +    L+++
Sbjct: 363 DYCIAKGAEAIDRATISPWPGTTLNLLKFPICNPTPYRMFQRQKRLKRDATEAEEDLSQQ 422

Query: 121 EQKLVHISKE 123
           EQ  +++ K+
Sbjct: 423 EQNKLNLLKK 432

[Close]

BLAST of MS025919 vs. ExPASy Swiss-Prot
Match: Q9JJG9 (Nitric oxide-associated protein 1 OS=Mus musculus OX=10090 GN=Noa1 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 7.4e-04
Identity = 26/98 (26.53%), Postives = 48/98 (48.98%), Query Frame = 0


Query: 3   SEGRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSI----- 62
           S  R  +  + ++SA   +G++ ++  +      RG+ + +G  NAGKSTL N++     
Sbjct: 306 SRSRTVVKDVRLISAKTGYGVEEMISALQRSWRYRGDVYLVGTTNAGKSTLFNTLLESDY 365

Query: 63  -GMHVGGKITQLTEAPVPGTTLGIIRVEGIRPGQAKLF 95
                   I + T +P PGTTL +++     P   ++F
Sbjct: 366 CTAKGSEAIDRATISPWPGTTLNLLKFPICNPTPYRMF 403

[Close]

BLAST of MS025919 vs. ExPASy TrEMBL
Match: A0A803PZS7 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.2e-77
Identity = 138/170 (81.18%), Postives = 157/170 (92.35%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  K+TS+H+VSA+RDWGL+NLV+DVI+LAG RGN WAIGAQNAGKSTLINS+G HVGGK
Sbjct: 286 GSNKLTSVHLVSAVRDWGLKNLVDDVIALAGQRGNVWAIGAQNAGKSTLINSLGKHVGGK 345

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           ++ LTEAPVPGTTLGI+RVEG+ P QAKLFDTPGLL+PHQI TRLTREEQKLV+ISKELK
Sbjct: 346 VSHLTEAPVPGTTLGIVRVEGVLPRQAKLFDTPGLLHPHQIATRLTREEQKLVYISKELK 405

Query: 125 PRTYRIKPPIGKDRVAELGKWVRREFRVCGSSWDSSWVDVAVAGLGWFAI 175
           PRTYRIKPPIGKDRV ELG+WVR+EF VCG+SWDSS VD++ AGLGWFAI
Sbjct: 406 PRTYRIKPPIGKDRVKELGQWVRKEFHVCGNSWDSSSVDISAAGLGWFAI 455

[Close]

BLAST of MS025919 vs. ExPASy TrEMBL
Match: A0A6J1DBW1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018893 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.0e-76
Identity = 156/226 (69.03%), Postives = 160/226 (70.80%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  KITSLHMVSA+RDWGL+NLVEDV+SL GPRGN WAIGAQNAGKSTLINSIGMHVGGK
Sbjct: 280 GMNKITSLHMVSAVRDWGLKNLVEDVVSLVGPRGNVWAIGAQNAGKSTLINSIGMHVGGK 339

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           ITQLTEA VPGTTLGIIRVEGI PGQAKLFDTPGLLNPHQITTRLTREEQKLVHI+KELK
Sbjct: 340 ITQLTEAAVPGTTLGIIRVEGILPGQAKLFDTPGLLNPHQITTRLTREEQKLVHINKELK 399

Query: 125 PRTYRIK----------------------------------------------------- 175
           PRTYRIK                                                     
Sbjct: 400 PRTYRIKAGHTVHVAGLMRLDVEETTVDSIYVTVWASPYLPLHMGKTENASKIQEDHFGN 459

[Close]

BLAST of MS025919 vs. ExPASy TrEMBL
Match: A0A6J1DAJ3 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018893 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.0e-76
Identity = 156/226 (69.03%), Postives = 160/226 (70.80%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  KITSLHMVSA+RDWGL+NLVEDV+SL GPRGN WAIGAQNAGKSTLINSIGMHVGGK
Sbjct: 186 GMNKITSLHMVSAVRDWGLKNLVEDVVSLVGPRGNVWAIGAQNAGKSTLINSIGMHVGGK 245

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           ITQLTEA VPGTTLGIIRVEGI PGQAKLFDTPGLLNPHQITTRLTREEQKLVHI+KELK
Sbjct: 246 ITQLTEAAVPGTTLGIIRVEGILPGQAKLFDTPGLLNPHQITTRLTREEQKLVHINKELK 305

Query: 125 PRTYRIK----------------------------------------------------- 175
           PRTYRIK                                                     
Sbjct: 306 PRTYRIKAGHTVHVAGLMRLDVEETTVDSIYVTVWASPYLPLHMGKTENASKIQEDHFGN 365

[Close]

BLAST of MS025919 vs. ExPASy TrEMBL
Match: A0A6J1FW06 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448990 PE=4 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 2.2e-75
Identity = 153/226 (67.70%), Postives = 159/226 (70.35%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  KITSLHMVSA+RDWGL++LVEDVI L GPRGN WA+GAQNAGKSTLINSIG HVGGK
Sbjct: 282 GINKITSLHMVSAVRDWGLKSLVEDVIDLVGPRGNVWAVGAQNAGKSTLINSIGKHVGGK 341

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           ITQLTEAPVPGTTLGIIRVEG+ PGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK
Sbjct: 342 ITQLTEAPVPGTTLGIIRVEGMFPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 401

Query: 125 PRTYRIK----------------------------------------------------- 175
           PRTYRIK                                                     
Sbjct: 402 PRTYRIKAGHTIHVAGLMRLDVEETSVDTIYVTVWASPYLPLHMGKTENASKILEDHFGN 461

[Close]

BLAST of MS025919 vs. ExPASy TrEMBL
Match: A0A0A0KZ58 (G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G285700 PE=4 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.9e-75
Identity = 155/226 (68.58%), Postives = 159/226 (70.35%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  KITSLHMVS+IRDWG++NLVEDVI LAG RGN WAIGAQNAGKSTLINSIG HVGGK
Sbjct: 268 GINKITSLHMVSSIRDWGIKNLVEDVIELAGARGNVWAIGAQNAGKSTLINSIGKHVGGK 327

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           ITQLTEAPVPGTTLGIIRVEGI P QAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK
Sbjct: 328 ITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 387

Query: 125 PRTYRIK----------------------------------------------------- 175
           PRTYRIK                                                     
Sbjct: 388 PRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVTVWASPYLPLHMGKTENATKMQEDHFGI 447

[Close]

BLAST of MS025919 vs. TAIR 10
Match: AT4G10620.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 249.6 bits (636), Expect = 1.8e-66
Identity = 127/226 (56.19%), Postives = 152/226 (67.26%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  KIT LH VS +++WG+++LVEDV ++AG RG+ WA+G+QNAGKSTLIN++G  VGGK
Sbjct: 281 GLSKITKLHFVSPVKNWGIKDLVEDVAAMAGKRGHVWAVGSQNAGKSTLINAVGKVVGGK 340

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           +  LTEAPVPGTTLGIIR+EG+ P +AKLFDTPGLLNPHQITTRLTREEQ+LVHISKELK
Sbjct: 341 VWHLTEAPVPGTTLGIIRIEGVLPFEAKLFDTPGLLNPHQITTRLTREEQRLVHISKELK 400

Query: 125 PRTYRIK----------------------------------------------------- 175
           PRTYRIK                                                     
Sbjct: 401 PRTYRIKEGYTVHIGGLMRLDIDEASVDSLYVTVWASPYVPLHMGKKENAYKTLEDHFGC 460

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BLAST of MS025919 vs. TAIR 10
Match: AT3G57180.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 184.5 bits (467), Expect = 7.3e-47
Identity = 94/226 (41.59%), Postives = 134/226 (59.29%), Query Frame = 0


Query: 5   GRRKITSLHMVSAIRDWGLQNLVEDVISLAGPRGNAWAIGAQNAGKSTLINSIGMHVGGK 64
           G  K++ ++MVSA +D G++NL+  +  LAGPRGN W IGAQNAGKSTLIN++    G K
Sbjct: 360 GAPKLSGVYMVSARKDIGVKNLLAYIKELAGPRGNVWVIGAQNAGKSTLINALSKKDGAK 419

Query: 65  ITQLTEAPVPGTTLGIIRVEGIRPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELK 124
           +T+LTEAPVPGTTLGI+++ GI   +AK++DTPGLL+P+ ++ RL  EE+K+V I KE++
Sbjct: 420 VTRLTEAPVPGTTLGILKIGGILSAKAKMYDTPGLLHPYLMSLRLNSEERKMVEIRKEVQ 479

Query: 125 PRTYRIK----------------------------------------------------- 175
           PR+YR+K                                                     
Sbjct: 480 PRSYRVKAGQSVHIGGLVRLDLVSASVETIYITIWASHSVSLHLGKTENAEEIFKGHSGL 539

The following BLAST results are available for this feature:

BLAST of MS025919 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150848.1	4.1e-76	69.03	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform...	[more]
XP_022150846.1	4.1e-76	69.03	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform...	[more]
XP_038901740.1	1.6e-75	68.89	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Beninc...	[more]
XP_023513264.1	2.0e-75	68.14	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Cucurb...	[more]
KAG6570359.1	3.5e-75	68.14	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic, partia...	[more]

BLAST of MS025919 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8W4I6	1.0e-45	41.59	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Arab...	[more]
P54453	2.5e-12	34.15	Uncharacterized protein YqeH OS=Bacillus subtilis (strain 168) OX=224308 GN=yqeH...	[more]
Q8NC60	1.5e-04	26.40	Nitric oxide-associated protein 1 OS=Homo sapiens OX=9606 GN=NOA1 PE=1 SV=2	[more]
Q32LB9	4.4e-04	24.62	Nitric oxide-associated protein 1 OS=Bos taurus OX=9913 GN=NOA1 PE=2 SV=1	[more]
Q9JJG9	7.4e-04	26.53	Nitric oxide-associated protein 1 OS=Mus musculus OX=10090 GN=Noa1 PE=1 SV=1	[more]

BLAST of MS025919 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A803PZS7	5.2e-77	81.18	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A6J1DBW1	2.0e-76	69.03	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform...	[more]
A0A6J1DAJ3	2.0e-76	69.03	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform...	[more]
A0A6J1FW06	2.2e-75	67.70	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucu...	[more]
A0A0A0KZ58	2.9e-75	68.58	G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G285700 PE=4 SV=1	[more]

BLAST of MS025919 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10620.1	1.8e-66	56.19	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT3G57180.1	7.3e-47	41.59	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006073	GTP binding domain	PFAM	PF01926	MMR_HSR1	coord: 43..115 e-value: 1.7E-9 score: 37.7
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 1..104 e-value: 1.1E-16 score: 63.1
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 7..107
None	No IPR available	PANTHER	PTHR46434:SF3	GTP-BINDING PROTEIN BRASSINAZOLE INSENSITIVE PALE GREEN 2, CHLOROPLASTIC	coord: 3..131 coord: 129..174
None	No IPR available	PANTHER	PTHR46434	GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL	coord: 129..174
None	No IPR available	PANTHER	PTHR46434	GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL	coord: 3..131

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS025919.1	MS025919.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005525	GTP binding