MS025918 (gene) Bitter gourd (TR) v1

Overview
NameMS025918
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionGTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic
Locationscaffold391: 1094307 .. 1094831 (+)
RNA-Seq ExpressionMS025918
SyntenyMS025918
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAACTACGACGAAGCCGCTTCCCAATTCTTCCTCGTCTGCCCTGGATGTGGAGTTCACATGCAAGACACCAAATCCCAACCATCCTGGTTTCTTCGTCAAACCCTCCAGAAAAGATTCCAATTATCGATTGCTTACGCATCTCGTGCCCGTTGATCACGAGTCTGAATGCTCCGATTCCCTGAAGAGAGGCCTCGTAATCGAGTGTGGGAATAAGAAAACGGAAGATAATGCGATCGAGAAAGCCCAAATGCCTATCGTATGCTCGCGGTGCCACTCATTGAGGTTTCATGGGAAGGTGAAGGGTCCTACAGTAGAGAATTTGTTACCGGATTTCGATTTCAATCATACTATTGGTAGGAGGCTGGCGTCGACTACGAGGACCCGATCTATCGTGTTGATGGTCGTTGAGGTCATTTTCAAAGAAGGTAGCAAATTTGGTGTCGGAGACAATTTGAGGAGAATTCAGCTGTCTGGAAACAGGGGAAATCAGGTAATGTGCCTAGAGTGGTGCTTGTGGTGA

mRNA sequence

ATGGCAACTACGACGAAGCCGCTTCCCAATTCTTCCTCGTCTGCCCTGGATGTGGAGTTCACATGCAAGACACCAAATCCCAACCATCCTGGTTTCTTCGTCAAACCCTCCAGAAAAGATTCCAATTATCGATTGCTTACGCATCTCGTGCCCGTTGATCACGAGTCTGAATGCTCCGATTCCCTGAAGAGAGGCCTCGTAATCGAGTGTGGGAATAAGAAAACGGAAGATAATGCGATCGAGAAAGCCCAAATGCCTATCGTATGCTCGCGGTGCCACTCATTGAGGTTTCATGGGAAGGTGAAGGGTCCTACAGTAGAGAATTTGTTACCGGATTTCGATTTCAATCATACTATTGGTAGGAGGCTGGCGTCGACTACGAGGACCCGATCTATCGTGTTGATGGTCGTTGAGGTCATTTTCAAAGAAGGTAGCAAATTTGGTGTCGGAGACAATTTGAGGAGAATTCAGCTGTCTGGAAACAGGGGAAATCAGGTAATGTGCCTAGAGTGGTGCTTGTGGTGA

Coding sequence (CDS)

ATGGCAACTACGACGAAGCCGCTTCCCAATTCTTCCTCGTCTGCCCTGGATGTGGAGTTCACATGCAAGACACCAAATCCCAACCATCCTGGTTTCTTCGTCAAACCCTCCAGAAAAGATTCCAATTATCGATTGCTTACGCATCTCGTGCCCGTTGATCACGAGTCTGAATGCTCCGATTCCCTGAAGAGAGGCCTCGTAATCGAGTGTGGGAATAAGAAAACGGAAGATAATGCGATCGAGAAAGCCCAAATGCCTATCGTATGCTCGCGGTGCCACTCATTGAGGTTTCATGGGAAGGTGAAGGGTCCTACAGTAGAGAATTTGTTACCGGATTTCGATTTCAATCATACTATTGGTAGGAGGCTGGCGTCGACTACGAGGACCCGATCTATCGTGTTGATGGTCGTTGAGGTCATTTTCAAAGAAGGTAGCAAATTTGGTGTCGGAGACAATTTGAGGAGAATTCAGCTGTCTGGAAACAGGGGAAATCAGGTAATGTGCCTAGAGTGGTGCTTGTGGTGA

Protein sequence

MATTTKPLPNSSSSALDVEFTCKTPNPNHPGFFVKPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIECGNKKTEDNAIEKAQMPIVCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLASTTRTRSIVLMVVEVIFKEGSKFGVGDNLRRIQLSGNRGNQVMCLEWCLW
Homology
BLAST of MS025918 vs. NCBI nr
Match: XP_022150848.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 213.0 bits (541), Expect = 2.1e-51
Identity = 99/120 (82.50%), Postives = 110/120 (91.67%), Query Frame = 0

Query: 26  NPNHPGFFVKPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIECGNKKTEDNAIEKAQM 85
           NPNHPGFFV+PSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIE GN+KTE+N I+KAQ+
Sbjct: 5   NPNHPGFFVQPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIESGNQKTEENVIKKAQI 64

Query: 86  PIVCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLASTTRTRSIVLMVVEVIFKEGS 145
           P+VCSRCHSLRF+GKVK PTVENLLPDFDF+HTIGRRL STT  RS+VLMVV+    +GS
Sbjct: 65  PVVCSRCHSLRFYGKVKDPTVENLLPDFDFDHTIGRRLVSTTGPRSVVLMVVDAADFDGS 124

BLAST of MS025918 vs. NCBI nr
Match: XP_022150846.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform X1 [Momordica charantia] >XP_022150847.1 GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 213.0 bits (541), Expect = 2.1e-51
Identity = 99/120 (82.50%), Postives = 110/120 (91.67%), Query Frame = 0

Query: 26  NPNHPGFFVKPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIECGNKKTEDNAIEKAQM 85
           NPNHPGFFV+PSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIE GN+KTE+N I+KAQ+
Sbjct: 99  NPNHPGFFVQPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIESGNQKTEENVIKKAQI 158

Query: 86  PIVCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLASTTRTRSIVLMVVEVIFKEGS 145
           P+VCSRCHSLRF+GKVK PTVENLLPDFDF+HTIGRRL STT  RS+VLMVV+    +GS
Sbjct: 159 PVVCSRCHSLRFYGKVKDPTVENLLPDFDFDHTIGRRLVSTTGPRSVVLMVVDAADFDGS 218

BLAST of MS025918 vs. NCBI nr
Match: KAG6570359.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010240.1 GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 189.9 bits (481), Expect = 1.9e-44
Identity = 90/120 (75.00%), Postives = 102/120 (85.00%), Query Frame = 0

Query: 26  NPNHPGFFVKPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIECGNKKTEDNAIEKAQM 85
           NP HPGFFVKPSRKDSNYRLLTHLVPVD ESECSD LKRGLV+E  ++K+E+N  EKAQ 
Sbjct: 101 NPKHPGFFVKPSRKDSNYRLLTHLVPVDDESECSDLLKRGLVMEPEHQKSEENVNEKAQK 160

Query: 86  PIVCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLASTTRTRSIVLMVVEVIFKEGS 145
           P VCSRCHSLR +GKVK P +ENLLPDFDF+HTIGRRL STT TRS+VL+VV+    +GS
Sbjct: 161 PTVCSRCHSLRHYGKVKDPALENLLPDFDFDHTIGRRLVSTTGTRSVVLLVVDAADFDGS 220

BLAST of MS025918 vs. NCBI nr
Match: XP_023513264.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 189.9 bits (481), Expect = 1.9e-44
Identity = 90/120 (75.00%), Postives = 102/120 (85.00%), Query Frame = 0

Query: 26  NPNHPGFFVKPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIECGNKKTEDNAIEKAQM 85
           NP HPGFFVKPSRKDSNYRLLTHLVPVD ESECSD LKRGLV+E  ++K+E+N  EKAQ 
Sbjct: 101 NPKHPGFFVKPSRKDSNYRLLTHLVPVDDESECSDLLKRGLVMEPEHQKSEENVNEKAQK 160

Query: 86  PIVCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLASTTRTRSIVLMVVEVIFKEGS 145
           P VCSRCHSLR +GKVK P +ENLLPDFDF+HTIGRRL STT TRS+VL+VV+    +GS
Sbjct: 161 PTVCSRCHSLRHYGKVKDPALENLLPDFDFDHTIGRRLVSTTGTRSVVLLVVDAADFDGS 220

BLAST of MS025918 vs. NCBI nr
Match: XP_022986065.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 189.5 bits (480), Expect = 2.4e-44
Identity = 90/120 (75.00%), Postives = 102/120 (85.00%), Query Frame = 0

Query: 26  NPNHPGFFVKPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIECGNKKTEDNAIEKAQM 85
           NP HPGFFVKPSRKDSNYRLLTHLVPVD ESEC D LKRGLV+E  ++K+E+N  EKAQ 
Sbjct: 101 NPKHPGFFVKPSRKDSNYRLLTHLVPVDDESECPDLLKRGLVMEPEHQKSEENVNEKAQK 160

Query: 86  PIVCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLASTTRTRSIVLMVVEVIFKEGS 145
           P VCSRCHSLR +GKVK P +ENLLPDFDF+HTIGRRLASTT TRS+VL+VV+    +GS
Sbjct: 161 PTVCSRCHSLRHYGKVKDPALENLLPDFDFDHTIGRRLASTTGTRSVVLLVVDAADFDGS 220

BLAST of MS025918 vs. ExPASy Swiss-Prot
Match: Q8W4I6 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BPG2 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 7.7e-09
Identity = 29/60 (48.33%), Postives = 41/60 (68.33%), Query Frame = 0

Query: 88  VCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLAS--TTRTRSIVLMVVEVIFKEGS 146
           VC+RCHSLR +G+VK    ENLLPDFDF+  I  RL    +  + ++V+MVV+ +  +GS
Sbjct: 241 VCARCHSLRNYGQVKNQAAENLLPDFDFDRLISTRLIKPMSNSSTTVVVMVVDCVDFDGS 300

BLAST of MS025918 vs. ExPASy TrEMBL
Match: A0A6J1DBW1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018893 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 9.9e-52
Identity = 99/120 (82.50%), Postives = 110/120 (91.67%), Query Frame = 0

Query: 26  NPNHPGFFVKPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIECGNKKTEDNAIEKAQM 85
           NPNHPGFFV+PSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIE GN+KTE+N I+KAQ+
Sbjct: 99  NPNHPGFFVQPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIESGNQKTEENVIKKAQI 158

Query: 86  PIVCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLASTTRTRSIVLMVVEVIFKEGS 145
           P+VCSRCHSLRF+GKVK PTVENLLPDFDF+HTIGRRL STT  RS+VLMVV+    +GS
Sbjct: 159 PVVCSRCHSLRFYGKVKDPTVENLLPDFDFDHTIGRRLVSTTGPRSVVLMVVDAADFDGS 218

BLAST of MS025918 vs. ExPASy TrEMBL
Match: A0A6J1DAJ3 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018893 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 9.9e-52
Identity = 99/120 (82.50%), Postives = 110/120 (91.67%), Query Frame = 0

Query: 26  NPNHPGFFVKPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIECGNKKTEDNAIEKAQM 85
           NPNHPGFFV+PSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIE GN+KTE+N I+KAQ+
Sbjct: 5   NPNHPGFFVQPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIESGNQKTEENVIKKAQI 64

Query: 86  PIVCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLASTTRTRSIVLMVVEVIFKEGS 145
           P+VCSRCHSLRF+GKVK PTVENLLPDFDF+HTIGRRL STT  RS+VLMVV+    +GS
Sbjct: 65  PVVCSRCHSLRFYGKVKDPTVENLLPDFDFDHTIGRRLVSTTGPRSVVLMVVDAADFDGS 124

BLAST of MS025918 vs. ExPASy TrEMBL
Match: A0A6J1JD02 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483924 PE=4 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 1.2e-44
Identity = 90/120 (75.00%), Postives = 102/120 (85.00%), Query Frame = 0

Query: 26  NPNHPGFFVKPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIECGNKKTEDNAIEKAQM 85
           NP HPGFFVKPSRKDSNYRLLTHLVPVD ESEC D LKRGLV+E  ++K+E+N  EKAQ 
Sbjct: 101 NPKHPGFFVKPSRKDSNYRLLTHLVPVDDESECPDLLKRGLVMEPEHQKSEENVNEKAQK 160

Query: 86  PIVCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLASTTRTRSIVLMVVEVIFKEGS 145
           P VCSRCHSLR +GKVK P +ENLLPDFDF+HTIGRRLASTT TRS+VL+VV+    +GS
Sbjct: 161 PTVCSRCHSLRHYGKVKDPALENLLPDFDFDHTIGRRLASTTGTRSVVLLVVDAADFDGS 220

BLAST of MS025918 vs. ExPASy TrEMBL
Match: A0A6J1FW06 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448990 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.0e-43
Identity = 89/120 (74.17%), Postives = 101/120 (84.17%), Query Frame = 0

Query: 26  NPNHPGFFVKPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIECGNKKTEDNAIEKAQM 85
           N  HPGFFVKPSRKDSNYRLLTHLVPVD ESECSD LKRGLV+E  ++K+E+N  EKAQ 
Sbjct: 101 NRKHPGFFVKPSRKDSNYRLLTHLVPVDDESECSDLLKRGLVMEPEHQKSEENVNEKAQK 160

Query: 86  PIVCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLASTTRTRSIVLMVVEVIFKEGS 145
           P VCSRCHSLR +GKVK P +ENLLPDFDF+HTIGRRL STT TRS+VL+VV+    +GS
Sbjct: 161 PTVCSRCHSLRHYGKVKDPALENLLPDFDFDHTIGRRLVSTTGTRSVVLLVVDAADFDGS 220

BLAST of MS025918 vs. ExPASy TrEMBL
Match: A0A0A0KZ58 (G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G285700 PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.9e-43
Identity = 87/120 (72.50%), Postives = 100/120 (83.33%), Query Frame = 0

Query: 26  NPNHPGFFVKPSRKDSNYRLLTHLVPVDHESECSDSLKRGLVIECGNKKTEDNAIEKAQM 85
           NP HPGFF+KPS+KDSNYRLLTHLVPVD ESECS+ LKRGLVIE   +KTE++  EK Q 
Sbjct: 87  NPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECSEFLKRGLVIEPEKEKTEEDVNEKPQK 146

Query: 86  PIVCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLASTTRTRSIVLMVVEVIFKEGS 145
           P VCSRCHSLR +GKVK P+VENLLPDFDFNHT+G RL STT TRS+VL+VV+    +GS
Sbjct: 147 PTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTLGGRLVSTTGTRSVVLIVVDAADFDGS 206

BLAST of MS025918 vs. TAIR 10
Match: AT4G10620.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 126.3 bits (316), Expect = 2.4e-29
Identity = 64/124 (51.61%), Postives = 83/124 (66.94%), Query Frame = 0

Query: 26  NPNHPGFFVKPS-RKDSNYRLLTHLVPVDHESECSDSLKRGLVIE---CGNKKTEDNAIE 85
           NP HPGFF+KPS  K  N   L  L P+  E E  DS+KRG +IE     +    D+   
Sbjct: 97  NPKHPGFFIKPSTEKQRNDLNLRDLTPISQEPEFIDSIKRGFIIEPISSSDLNPRDDEPS 156

Query: 86  KAQMPIVCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLASTTRTRSIVLMVVEVIF 145
            ++ P+VC+RCHSLR +G+VK PTVENLLPDFDF+HT+GRRL S +  R++VLMVV+   
Sbjct: 157 DSR-PLVCARCHSLRHYGRVKDPTVENLLPDFDFDHTVGRRLGSASGARTVVLMVVDASD 216

BLAST of MS025918 vs. TAIR 10
Match: AT3G57180.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 5.5e-10
Identity = 29/60 (48.33%), Postives = 41/60 (68.33%), Query Frame = 0

Query: 88  VCSRCHSLRFHGKVKGPTVENLLPDFDFNHTIGRRLAS--TTRTRSIVLMVVEVIFKEGS 146
           VC+RCHSLR +G+VK    ENLLPDFDF+  I  RL    +  + ++V+MVV+ +  +GS
Sbjct: 241 VCARCHSLRNYGQVKNQAAENLLPDFDFDRLISTRLIKPMSNSSTTVVVMVVDCVDFDGS 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150848.12.1e-5182.50GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform... [more]
XP_022150846.12.1e-5182.50GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform... [more]
KAG6570359.11.9e-4475.00GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic, partia... [more]
XP_023513264.11.9e-4475.00GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Cucurb... [more]
XP_022986065.12.4e-4475.00GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Cucurb... [more]
Match NameE-valueIdentityDescription
Q8W4I67.7e-0948.33GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Arab... [more]
Match NameE-valueIdentityDescription
A0A6J1DBW19.9e-5282.50GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform... [more]
A0A6J1DAJ39.9e-5282.50GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform... [more]
A0A6J1JD021.2e-4475.00GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucu... [more]
A0A6J1FW061.0e-4374.17GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucu... [more]
A0A0A0KZ582.9e-4372.50G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G285700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G10620.12.4e-2951.61P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G57180.15.5e-1048.33P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR46434GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIALcoord: 23..139

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS025918.1MS025918.1mRNA