MS025910 (gene) Bitter gourd (TR) v1

Overview
NameMS025910
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionprotein TRIGALACTOSYLDIACYLGLYCEROL 5, chloroplastic
Locationscaffold391: 928812 .. 929213 (-)
RNA-Seq ExpressionMS025910
SyntenyMS025910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACGCCGATTCCAGATCGCCATGGAACTGGAAAGGAATTTCAAACAATTATCAGAGGAAGGACCAAAACAAATTCTCTCTCCCCAACATTTTCGACGCCAATCGCCGGAAGCCCAATGAACCAATACTAGTCTTAGAGGCCGGCGGAGGAGCGGGGGTAGGCTGCGGCGTCGGCCTTGGTTTCGGTCTGGTCGGCGCCGGCTGGATTGGCCTCGGCGGCAACCTTCACCTGGTCTTCGGGCTCGATCTCGGCTGCGGCGTTGGCCTAGGGCTTGCAATCGGCCAAGGGATTGGCTACGGCTTTAGCTCCGAATCTTTACATTCTTATTTCTCGGATGATAACTCTGATTCCAAACATAGGCCTCTCATTCCAAACCTAAAATATCTAAAAAGAATTTAA

mRNA sequence

ATGAACGCCGATTCCAGATCGCCATGGAACTGGAAAGGAATTTCAAACAATTATCAGAGGAAGGACCAAAACAAATTCTCTCTCCCCAACATTTTCGACGCCAATCGCCGGAAGCCCAATGAACCAATACTAGTCTTAGAGGCCGGCGGAGGAGCGGGGGTAGGCTGCGGCGTCGGCCTTGGTTTCGGTCTGGTCGGCGCCGGCTGGATTGGCCTCGGCGGCAACCTTCACCTGGTCTTCGGGCTCGATCTCGGCTGCGGCGTTGGCCTAGGGCTTGCAATCGGCCAAGGGATTGGCTACGGCTTTAGCTCCGAATCTTTACATTCTTATTTCTCGGATGATAACTCTGATTCCAAACATAGGCCTCTCATTCCAAACCTAAAATATCTAAAAAGAATTTAA

Coding sequence (CDS)

ATGAACGCCGATTCCAGATCGCCATGGAACTGGAAAGGAATTTCAAACAATTATCAGAGGAAGGACCAAAACAAATTCTCTCTCCCCAACATTTTCGACGCCAATCGCCGGAAGCCCAATGAACCAATACTAGTCTTAGAGGCCGGCGGAGGAGCGGGGGTAGGCTGCGGCGTCGGCCTTGGTTTCGGTCTGGTCGGCGCCGGCTGGATTGGCCTCGGCGGCAACCTTCACCTGGTCTTCGGGCTCGATCTCGGCTGCGGCGTTGGCCTAGGGCTTGCAATCGGCCAAGGGATTGGCTACGGCTTTAGCTCCGAATCTTTACATTCTTATTTCTCGGATGATAACTCTGATTCCAAACATAGGCCTCTCATTCCAAACCTAAAATATCTAAAAAGAATTTAA

Protein sequence

MNADSRSPWNWKGISNNYQRKDQNKFSLPNIFDANRRKPNEPILVLEAGGGAGVGCGVGLGFGLVGAGWIGLGGNLHLVFGLDLGCGVGLGLAIGQGIGYGFSSESLHSYFSDDNSDSKHRPLIPNLKYLKRI
Homology
BLAST of MS025910 vs. NCBI nr
Match: KAG6578355.1 (hypothetical protein SDJN03_22803, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 142.1 bits (357), Expect = 3.4e-30
Identity = 83/130 (63.85%), Postives = 95/130 (73.08%), Query Frame = 0

Query: 1   MNADSRSPWNWK-GISNNYQRK-DQNKFSLPNIFDANRRKPNE-PILVLEAGGGAGVGCG 60
           M +DS SPW W   I+NN+  K D + FSLPNIFDANRR   + P LVL+AGGGAGVGCG
Sbjct: 1   MKSDSNSPWKWNTAITNNFHTKADHHHFSLPNIFDANRRNAKQPPPLVLDAGGGAGVGCG 60

Query: 61  VGLGFGLVGAGWIGLGG-----NLHLVFGLDLGCGVGLGLAIGQGIGYGFSSESLHSYFS 120
           VGLGFGLVG   IG GG     +LHLVFGL LGCGVGLGL IG+GIGYG S +SL SYFS
Sbjct: 61  VGLGFGLVGG--IGHGGASPWNHLHLVFGLGLGCGVGLGLGIGKGIGYGISFDSLDSYFS 120

Query: 121 DDNSDSKHRP 123
           D   + + +P
Sbjct: 121 DHKPNPRQKP 128

BLAST of MS025910 vs. NCBI nr
Match: GAV90122.1 (hypothetical protein CFOL_v3_33531 [Cephalotus follicularis])

HSP 1 Score: 110.5 bits (275), Expect = 1.1e-20
Identity = 64/126 (50.79%), Postives = 81/126 (64.29%), Query Frame = 0

Query: 3   ADSRSPWNWKGISNNYQRKDQNKFSLPNIFDANRRKPNEPILVLEAGGGAGVGCGVGLGF 62
           +DS S WNW G +N  +RKD N F +       R+K     +V   GGG G+GCG G+GF
Sbjct: 10  SDSTSLWNWNG-NNKKKRKDGNWFPVAEFLGETRQKME--AIVDGPGGGIGIGCGFGVGF 69

Query: 63  GLVGAGWIGLGGNLHLVFGLDLGCGVGLGLAIGQGIGYGFSSESLHSYFSDDNSDSKHRP 122
           G+VG   IG GG++  VFG+ LGCGVG+G   GQGIGYGFS +SL SYF +   DSK RP
Sbjct: 70  GMVGG--IGFGGHVKPVFGVGLGCGVGVGFGYGQGIGYGFSIKSLESYFFEQRPDSKKRP 129

Query: 123 LIPNLK 129
           LI  ++
Sbjct: 130 LIEKVR 130

BLAST of MS025910 vs. NCBI nr
Match: KAA0051071.1 (fibroin heavy chain [Cucumis melo var. makuwa] >TYK02741.1 fibroin heavy chain [Cucumis melo var. makuwa])

HSP 1 Score: 107.5 bits (267), Expect = 9.3e-20
Identity = 72/125 (57.60%), Postives = 77/125 (61.60%), Query Frame = 0

Query: 5   SRSPWNWKGISNNYQRKDQNKFSLPNIFDANRRKPNE---PILVLEAGGGAGVGCGVGLG 64
           S SPWN             N  +    FD +RR   E     LVLEAGGGAGVGCG+G G
Sbjct: 6   SSSPWN-------------NFHTTIFSFDHDRRNTKELPALALVLEAGGGAGVGCGLGFG 65

Query: 65  FGLVGAGWIGLGG-----NLHLVFGLDLGCGVGLGLAIGQGIGYGFSSESLHSYFSDDNS 122
           FGLVG   IG GG     +LHLVFGL  GCGVGLGL IGQG GYGFS ESL SYFS  NS
Sbjct: 66  FGLVGG--IGHGGASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGFSFESLDSYFSHLNS 115

BLAST of MS025910 vs. NCBI nr
Match: XP_010108896.1 (keratin, type II cytoskeletal 5 [Morus notabilis] >EXC20536.1 hypothetical protein L484_027090 [Morus notabilis])

HSP 1 Score: 104.8 bits (260), Expect = 6.0e-19
Identity = 63/125 (50.40%), Postives = 77/125 (61.60%), Query Frame = 0

Query: 4   DSRSPWNWKGISNNYQRKDQNKFSLPNIFDANRRKPNEPILVLEAGGGAGVGCGVGLGFG 63
           DS + WNW     N +R  + +F +    D +R++  E   VL  G GAG+GCGVGLGFG
Sbjct: 11  DSNALWNWNESKLNKKRNSEKRFPVAEFLDRSRQRAQES--VLGPGAGAGIGCGVGLGFG 70

Query: 64  LVGAGWIGLGG----NLHLVFGLDLGCGVGLGLAIGQGIGYGFSSESLHSYFSDDNSDSK 123
           LVG    G GG    +L LV G+ LGCGVGLG+  GQG GYG+S ESL S  SD +SDS 
Sbjct: 71  LVGG--FGFGGPQWNHLKLVLGIGLGCGVGLGIGFGQGFGYGYSLESLESDLSDHDSDSN 130

Query: 124 HRPLI 125
            R LI
Sbjct: 131 KRGLI 131

BLAST of MS025910 vs. NCBI nr
Match: XP_034209523.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 5, chloroplastic [Prunus dulcis] >VVA33668.1 PREDICTED: keratin type II cytoskeletal [Prunus dulcis])

HSP 1 Score: 103.6 bits (257), Expect = 1.3e-18
Identity = 58/123 (47.15%), Postives = 84/123 (68.29%), Query Frame = 0

Query: 1   MNADSRSPWNWKGISNNYQRKDQNKFSLPNIFDANRRKPNEPILVLEAGGGAGVGCGVGL 60
           +NAD+ S W WK    N +++++N+FS+  IF ++++   E  ++L  G GAG+GCG G+
Sbjct: 7   INADTDSFWTWKPSPFNEKQQNENRFSIAEIFQSSQKA--ERKVLLGPGAGAGIGCGAGV 66

Query: 61  GFGLVGAGWIGLG----GNLHLVFGLDLGCGVGLGLAIGQGIGYGFSSESLHSYFSDDNS 120
           GFGLVG   +G G      + LVFG+ LGCG+G+G   GQG+GYG SS+SL SY S ++S
Sbjct: 67  GFGLVGG--LGYGAWPFNQVKLVFGVGLGCGIGVGFGFGQGVGYGVSSDSLESYVSKESS 125

BLAST of MS025910 vs. ExPASy TrEMBL
Match: A0A1Q3DCD5 (Uncharacterized protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_33531 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 5.3e-21
Identity = 64/126 (50.79%), Postives = 81/126 (64.29%), Query Frame = 0

Query: 3   ADSRSPWNWKGISNNYQRKDQNKFSLPNIFDANRRKPNEPILVLEAGGGAGVGCGVGLGF 62
           +DS S WNW G +N  +RKD N F +       R+K     +V   GGG G+GCG G+GF
Sbjct: 10  SDSTSLWNWNG-NNKKKRKDGNWFPVAEFLGETRQKME--AIVDGPGGGIGIGCGFGVGF 69

Query: 63  GLVGAGWIGLGGNLHLVFGLDLGCGVGLGLAIGQGIGYGFSSESLHSYFSDDNSDSKHRP 122
           G+VG   IG GG++  VFG+ LGCGVG+G   GQGIGYGFS +SL SYF +   DSK RP
Sbjct: 70  GMVGG--IGFGGHVKPVFGVGLGCGVGVGFGYGQGIGYGFSIKSLESYFFEQRPDSKKRP 129

Query: 123 LIPNLK 129
           LI  ++
Sbjct: 130 LIEKVR 130

BLAST of MS025910 vs. ExPASy TrEMBL
Match: A0A5A7UC28 (Fibroin heavy chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1154G00440 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 4.5e-20
Identity = 72/125 (57.60%), Postives = 77/125 (61.60%), Query Frame = 0

Query: 5   SRSPWNWKGISNNYQRKDQNKFSLPNIFDANRRKPNE---PILVLEAGGGAGVGCGVGLG 64
           S SPWN             N  +    FD +RR   E     LVLEAGGGAGVGCG+G G
Sbjct: 6   SSSPWN-------------NFHTTIFSFDHDRRNTKELPALALVLEAGGGAGVGCGLGFG 65

Query: 65  FGLVGAGWIGLGG-----NLHLVFGLDLGCGVGLGLAIGQGIGYGFSSESLHSYFSDDNS 122
           FGLVG   IG GG     +LHLVFGL  GCGVGLGL IGQG GYGFS ESL SYFS  NS
Sbjct: 66  FGLVGG--IGHGGASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGFSFESLDSYFSHLNS 115

BLAST of MS025910 vs. ExPASy TrEMBL
Match: W9S8Y9 (Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_027090 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.9e-19
Identity = 63/125 (50.40%), Postives = 77/125 (61.60%), Query Frame = 0

Query: 4   DSRSPWNWKGISNNYQRKDQNKFSLPNIFDANRRKPNEPILVLEAGGGAGVGCGVGLGFG 63
           DS + WNW     N +R  + +F +    D +R++  E   VL  G GAG+GCGVGLGFG
Sbjct: 11  DSNALWNWNESKLNKKRNSEKRFPVAEFLDRSRQRAQES--VLGPGAGAGIGCGVGLGFG 70

Query: 64  LVGAGWIGLGG----NLHLVFGLDLGCGVGLGLAIGQGIGYGFSSESLHSYFSDDNSDSK 123
           LVG    G GG    +L LV G+ LGCGVGLG+  GQG GYG+S ESL S  SD +SDS 
Sbjct: 71  LVGG--FGFGGPQWNHLKLVLGIGLGCGVGLGIGFGQGFGYGYSLESLESDLSDHDSDSN 130

Query: 124 HRPLI 125
            R LI
Sbjct: 131 KRGLI 131

BLAST of MS025910 vs. ExPASy TrEMBL
Match: A0A2N9FV55 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS19064 PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 6.5e-19
Identity = 65/130 (50.00%), Postives = 84/130 (64.62%), Query Frame = 0

Query: 1   MNADSRSPWN-WKGISNNYQRKDQNKFSLPNIFDANRRKPNEPILVLEAGGGAGVGCGVG 60
           + ADS S WN W G ++N ++ ++ +F +      NR K  E  +VL  GGG G+GCGVG
Sbjct: 8   IKADSNSFWNIWNGNNSNKKKNEKKQFPVSKFLHNNRMKAEE--IVLGPGGGVGIGCGVG 67

Query: 61  LGFGLVGAGWIGLGG----NLHLVFGLDLGCGVGLGLAIGQGIGYGFSSESLHSYFS-DD 120
           +GFGLVG   IG GG    +L LVFG+ +GCGVG+G   GQGIG GFS +SL SY S   
Sbjct: 68  MGFGLVGG--IGYGGWPWNHLQLVFGVGMGCGVGVGFGYGQGIGNGFSLDSLESYLSKQS 127

Query: 121 NSDSKHRPLI 125
           +SDSK R +I
Sbjct: 128 SSDSKKRVVI 133

BLAST of MS025910 vs. ExPASy TrEMBL
Match: A0A5N6QWW9 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_007887 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.4e-18
Identity = 61/128 (47.66%), Postives = 79/128 (61.72%), Query Frame = 0

Query: 1   MNADSRSPWNWKGISNNYQRKDQNKFSLPNIFDANRRKPNEPILVLEAGGGAGVGCGVGL 60
           + ADS S W W G  +N ++ ++ +F +   F   R    E  +VL  G G G+GCGVG+
Sbjct: 8   IKADSDSIWKWNGNLSNKKKNEKKQFPVAKFFQNGREAAEE--VVLGPGVGVGIGCGVGM 67

Query: 61  GFGLVGAGWIGLGG----NLHLVFGLDLGCGVGLGLAIGQGIGYGFSSESLHSYFSDDNS 120
           GFGLVG   IG GG    +L +VFG  +GCGVGLG   GQGIGYGFS ESL SY S  +S
Sbjct: 68  GFGLVGG--IGYGGWPWNHLQVVFGAGIGCGVGLGFGYGQGIGYGFSLESLESYTSKRSS 127

Query: 121 DSKHRPLI 125
           DS  + ++
Sbjct: 128 DSNKKKIL 131

BLAST of MS025910 vs. TAIR 10
Match: AT1G66820.1 (glycine-rich protein )

HSP 1 Score: 45.4 bits (106), Expect = 4.0e-05
Identity = 27/66 (40.91%), Postives = 37/66 (56.06%), Query Frame = 0

Query: 49  GGGAGVGCGVGLGFGLVGAGWIGLGGNL---HLVFGLDLGCGVGLGLAIGQGIGYGFSSE 108
           G G G+GCG G+G GL G   IG    L   ++V G  +GCG+G G   G G+G G+S +
Sbjct: 42  GIGGGIGCGAGIGIGLSGGLGIGASEGLDHSNVVLGFGIGCGIGFGFGYGFGVGGGYSFD 101

Query: 109 SLHSYF 112
            +   F
Sbjct: 102 DIKERF 107

BLAST of MS025910 vs. TAIR 10
Match: AT4G10330.1 (glycine-rich protein )

HSP 1 Score: 44.3 bits (103), Expect = 9.0e-05
Identity = 28/63 (44.44%), Postives = 37/63 (58.73%), Query Frame = 0

Query: 51  GAGVGCGVGLGFGLVGAGWIGLG-GNLHLVFGLDLGCGVGLGLAIGQGIGYGFSSESLHS 110
           G GVGCG G G GL+G   +G G G   L FGL  G G G+G+  G G+G G + +   S
Sbjct: 34  GLGVGCGFGFGAGLIGG--VGFGPGVPGLQFGLGFGAGCGIGVGFGYGVGRGAAYDHSRS 93

Query: 111 YFS 113
           Y++
Sbjct: 94  YYN 94

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6578355.13.4e-3063.85hypothetical protein SDJN03_22803, partial [Cucurbita argyrosperma subsp. sorori... [more]
GAV90122.11.1e-2050.79hypothetical protein CFOL_v3_33531 [Cephalotus follicularis][more]
KAA0051071.19.3e-2057.60fibroin heavy chain [Cucumis melo var. makuwa] >TYK02741.1 fibroin heavy chain [... [more]
XP_010108896.16.0e-1950.40keratin, type II cytoskeletal 5 [Morus notabilis] >EXC20536.1 hypothetical prote... [more]
XP_034209523.11.3e-1847.15protein TRIGALACTOSYLDIACYLGLYCEROL 5, chloroplastic [Prunus dulcis] >VVA33668.1... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1Q3DCD55.3e-2150.79Uncharacterized protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_33531 PE=4... [more]
A0A5A7UC284.5e-2057.60Fibroin heavy chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1154... [more]
W9S8Y92.9e-1950.40Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_027090 PE=4 SV=1[more]
A0A2N9FV556.5e-1950.00Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS19064 PE=4 SV=1[more]
A0A5N6QWW91.4e-1847.66Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_007887 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G66820.14.0e-0540.91glycine-rich protein [more]
AT4G10330.19.0e-0544.44glycine-rich protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34201GLYCINE-RICH PROTEINcoord: 3..121
NoneNo IPR availablePANTHERPTHR34201:SF6GLYCINE-RICH PROTEINcoord: 3..121

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS025910.1MS025910.1mRNA