MS025881 (gene) Bitter gourd (TR) v1

Overview
NameMS025881
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionhydrolase family protein / HAD-superfamily protein
Locationscaffold779: 139870 .. 144453 (-)
RNA-Seq ExpressionMS025881
SyntenyMS025881
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAATCCTTACGGTCTTCAGAGTCTCTCAAGCCAAAATTGGAGTGCCATTTTCGTTTCAATCTCAGGATTCTGCTTCTCGTTCATTCTCCCGGATTCAATCTCCACCTGAACGGTTTGCCGTCTTAACCTTACTCATAATTTTCCGTATTTTGGTTGCTTTATTCTTGATTGGCTACTCAGAAATCGTTGTAAAGGACTGTAGTATGTTTTCAAGAAAGTTCGCGGGCAGTAACAGAGATTCGCATCTTTAGTTTCGCGATTGAAACTCTGCGAGAATAGGATATTAGGAACTACATTTCAATTCCATTTTCTTTTAGGAGACTACGTACTATGCTATTTTGATCAGAAAGATGATATTTATCATTTATTATTGCTGTTCGTAGTTTCGTTTATTTATTTACTGGCAACCAAGAAGTGAAAAGAGCTTATGATGATCACAGTTCTTCTTTCGGGATTGCATTTGACATCGATGGGGTTGTTCTACGTGGCCACACTCCTATTGGAGGGTCTCCCAAAGCTTTGAGAAGGCTGTATGCGGATTCTACATCCTCTGGTATCGATCTATCCACCGAAGTGAAATTTTCAAACTATAAAAACCAGAAAATCATCAAGGTGCTGCCCTTTTCTTGGTTTCTACAAAAAATAACGTTTTCTTCAGGTGGAAACACATGAATAGCAGTTTATTTTTATTTTTCTCAACAAATCGCCTGGAAGTTGATTTTGGGGACTGCCTAATCAAGAGTGGAACAACAAGAGTTGCCATCTACTTGATTACTAAATTGATGTTATGTAACATAGTCATTTTTTCTTGTTTTGGTGTATATTGTTTTGCTTTATTGCTTTTTGGGCATGGTTCGGTAAATTGACTCTGTATCTAATACAAATGATGCTGCTGTTGTGTAGGTCCTTTGAAAGTTCCTTTTCTTTTCTTGACCAATGGTGAGTTTCTCGTCCTTTCTTCATGGTATTCAATGTTGTCTTATAGTTAATTTGTTGAAACGAACTCACAATGTATGTATACCACTGTTCAACTATTCCGTGAAATGGATTAGCCAGCAATATGAGCCAATGGTTGAGTGCCTATATATGAGGGAAAAAAGCCTTATTATTGCATTTTTGCCAGCTTTTGTAATTGCATCTGATGCACAAAAAATTGTTTGACAACTTATTCTTGCGAATACTTACCATGGTTATTAGCACAACATTTTCCCCCAACCTCACCAAAAGATATGCTAGTTTCTGATGCTATTGTAAGTGTACAGCTATTTCATGACAGTTATAGAGTTCCTCGTGATCGTTTTTACTTGCTGAGCATTATATCCAATTATACATTGTGAAATAATTATAAAAGATAGTTATTTAGCATATAAAAGATGACAATATTGTTATTCATTTTCTTGTGTAGGAGGAGGTACCCCAGAGTCCAGACGCTCCATTGAATTGAGTGACCTTTTAGGAGTGAATATATTACCTTCTCAGGTAGTTATTTCTCACATTACGATCCTCCCTTTTATACTGAATGAGATGCTGCTTTCTCAGCAATTTATTGATTTTGTCGGCTTTGTCGTAGCTCTCATTTTTTTATATCTTGATGCAGGTTGTACAGGGCCACTCAAGTTTTAAAAGTTTGTTGAGTAGGTACCTTCATATAAATTTGGAGGGTACTTTTTCCTTAACATATCTTTTGAAGTAGGAAAAGAAGAAAAAGGAAAAAAAATTAGTACAATGAAACTCACTATACTTCATATAAGGTTCTGCAGAAGATAAAACTAGCTTATGCAGTTGCTACTTAAAAAAGTTTTCAGAGATTTCTTCGAACAGCTTTCTCCTCTGTATATTATTAAGCGATAGGAATTTTTGTCCCCCTACTAAAAAAGAAAGGGAAAGGCCATTTGAAGGTTGTTTTTAGGTTAGCATGAATATAGTGAAAGTGAGCTAAAACTGAATCTTGGAATGACATCATGGATAAATTTTTTTAGATTACTGAAGCTTTGAAATAGATACCTTAGGGACCTGGGCAATAAGTTTGGTGAGGTCTTCTTATTTACCATGCTAAACTCACAGATTTTAGCTTATCTTATATTGTCAACGATATTTTGCATCATGTTTCTTATTATCGTCGGCATATGCTTTAAATTTTGTACTTAACTGCTTTTGTTTACCTCAGGTTTGAGAATGAACTTATCGTTGCAACTGGAAAGGGTGAGCCAGCTCTTGTTATGTCTGAGTATGGTTTCAAGTAAGCTCTTAGCCATATGTCTTGGATTATCTTTCGTCCAATGCCTTCAGAGCTAGTTGGATCACATATGATAATATTTTATTCCTCTACAGAAAAGTTTTCTCCATTGATGAGTATGCTTCCTTCTTTGAGAATATTGATCCTGTATCCCAATACAAGAACTGGACAACCAAACAAGATTTTGATTTGACTAGCAACCCTGTGAAGTTGATGAGGAGAAGGCAGAGTGTTTTCTCTGAAAGAGTGAAAGCGGCTTTTGTTGTCAGTGATCCTGTTGATTGGGGCAGGGATATCCAAGTACTCATTCTCTCTCTTTTATTCTTTGAGTTTCTGAGCTGGTAGTTGTATAGTGGGTTGTAAATTTAGCTGTTAAGGCTACTTGTTCCAAACTTCCATTAGGTAAATTTTACTTCATGTCTATAAATTTGAGTTACTAACATTTGTCCAGCGACAAATAATTATTATAGGTTTTGAAATTACTTGGCTCTTCAATAGTAACATCTGTCTAGGACTTTGTATAATTTACTGGGTAATAAATTAAAAAATTTTCTCCAGTTCGATACCTCCGTAGACTTCTAATTCATTTCACTTGTAGATAAATTGTGAATCAATCGATGAAAATTTAGATTGCTAATCATTGTACACGAATATAATATATTTTATTGTAAAATATTGTTGAAGATCTATCATTTATTATTGAAATTGTGGAAATTTGATACTGCCGCCCACCTGAAAAGTTCAGATTCTTAATTAAAGATTGTACATCTTTCAATCCATCCAAAAAAAAAAAAAAAAATCTTCAATAGTTTGTTGAACTTTTATGGTAGTTGAAATTGCAAGATGTTAATACCTTGTGTTACCAAAACAGAATAAAATATTGAACTATTGCAATGCTTCCCACAGTAGTAGTCTTCGCCCCATAACCTTTAACCATTTCTGATTTGCTTTCTTAGTAAATTTGAAGCTTGGTTTTCCTTTTTCCCTTTTGAGTTTCCTTGACCAGGTTTACTACTAAGATTCAGATTCAAAGAAATTAGCTTGAAATCGAACTTTGAATTCAGCCCCCTTGCTTGATGCCGACCTCTTGATAATAGCTATTCTTGCAAAAGCTTCAAACTCATTGATGAATGAATTAGGTTCTTTGTGATATTCTAAGATCTGGAGGCCTTCCAGGACAGGAGAATGGGAATCAGGTGCCTCTATATTTTGCTGCAGATGATTTTGAATATCAGGTTAGTTTCAAAGATTATGTTATGTATTACTTATGCAAATAATGCTTATCGATTGAAATAACCGCCAGCACAGGGTTACATCTCCTAATGGTGCCGTGCAATTGTTTGTCATTTCTCTTACTCTGCATCTACCTAGCCTACCATTCTCTCCATTAGTTTTTAAGGCTACAAATGCCATTGCCAGTAAAATCGAGAATTTGTCGATTGGATTTTTTGTGCAGGCTGCATTTCCGTTTAAGCGGCTTGGGATAGGTGCCTTTAAAATTGCACTGGAAAGTATATTTAATAGGTAAAGTTGCTATCTTGTGTCAAAATTTTCTCCATTTGCTGCTTAAAACTCAGGAAACATGGCAATATTTTGGCCCATGAATTGCCCCTTGGTTCCTAGGTGTTCGAGATTCTCTCTCTTTTCATTCACTTACAAGCTGTGTTAGAAGTGCTACTAATTTTTATGGTTTAAAAGCTAATTCCCTCGTGTTAGAATTATTACCACTCCAAGTATTGATTAAAACTGTTTGATCTTGAGTCGCAGGATTCACCATAGACCTCTCGAATATGTAACTCACGGGAAACCAAATCCTCTTGTGTTTAAGAATGTTGAAGTTCTGCTGAGACAAATTCTCTCATCTCACTGTGATGATCATTTTGTGAACAATCGAGATATGGAATTTACTCGTTTTAAAACACTTTATATGATCGGCGACAACCCCGCGGTAGACATCAAAGGTGCACGAGAGGTTCGTCATTTTAGCACTTGTAGTTTCATCACCTTATTGTCATTGCGAATAAATATTTCGCATTGTGAAAACCAGATTTTAATATCCAAAATCGACTTGTGATTTCAGGCAGGACGACCTTGGTTTTCTATTTTAACTAGGACAGGAGTGTTCAGGGGCGAAGAAAATCACGCCGAGTTTCCTGCCGATCTTGTAAGTAGAAAGTTGATGGATAAAAATGCATACAGAACTTACAAGTATTACAATGTAATCACATTAATAATGTCTGTCCTATTACCTTCTATATTTGAGTTTCTTTTTCGATTCTTGCAGGTCGTTGATACCATTGAAGAAGCAGTGGACTATATTTTCGAAACTGAGTGCATTTCTTAA

mRNA sequence

ATGAAAATCCTTACGGTCTTCAGAGTCTCTCAAGCCAAAATTGGAGTGCCATTTTCGTTTCAATCTCAGGATTCTGCTTCTCGTTCATTCTCCCGGATTCAATCTCCACCTGAACGTTTCGTTTATTTATTTACTGGCAACCAAGAAGTGAAAAGAGCTTATGATGATCACAGTTCTTCTTTCGGGATTGCATTTGACATCGATGGGGTTGTTCTACGTGGCCACACTCCTATTGGAGGGTCTCCCAAAGCTTTGAGAAGGCTGTATGCGGATTCTACATCCTCTGGAGGAGGTACCCCAGAGTCCAGACGCTCCATTGAATTGAGTGACCTTTTAGGAGTGAATATATTACCTTCTCAGGTTGTACAGGGCCACTCAAGTTTTAAAAGTTTGTTGAGTAGGTTTGAGAATGAACTTATCGTTGCAACTGGAAAGGGTGAGCCAGCTCTTGTTATGTCTGAGTATGGTTTCAAAAAAGTTTTCTCCATTGATGAGTATGCTTCCTTCTTTGAGAATATTGATCCTGTATCCCAATACAAGAACTGGACAACCAAACAAGATTTTGATTTGACTAGCAACCCTGTGAAGTTGATGAGGAGAAGGCAGAGTGTTTTCTCTGAAAGAGTGAAAGCGGCTTTTGTTGTCAGTGATCCTGTTGATTGGGGCAGGGATATCCAAGTTCTTTGTGATATTCTAAGATCTGGAGGCCTTCCAGGACAGGAGAATGGGAATCAGGTGCCTCTATATTTTGCTGCAGATGATTTTGAATATCAGCACAGGGTTACATCTCCTAATGGTGCCGTGCAATTGTTTGTCATTTCTCTTACTCTGCATCTACCTAGCCTACCATTCTCTCCATTAGTTTTTAAGGCTACAAATGCCATTGCCAGTAAAATCGAGAATTTGTCGATTGGATTTTTTGTGCAGGCTGCATTTCCGTTTAAGCGGCTTGGGATAGGTGCCTTTAAAATTGCACTGGAAAGTATATTTAATAGGATTCACCATAGACCTCTCGAATATGTAACTCACGGGAAACCAAATCCTCTTGTGTTTAAGAATGTTGAAGTTCTGCTGAGACAAATTCTCTCATCTCACTGTGATGATCATTTTGTGAACAATCGAGATATGGAATTTACTCGTTTTAAAACACTTTATATGATCGGCGACAACCCCGCGGTAGACATCAAAGGTGCACGAGAGGCAGGACGACCTTGGTTTTCTATTTTAACTAGGACAGGAGTGTTCAGGGGCGAAGAAAATCACGCCGAGTTTCCTGCCGATCTTGTCGTTGATACCATTGAAGAAGCAGTGGACTATATTTTCGAAACTGAGTGCATTTCTTAA

Coding sequence (CDS)

ATGAAAATCCTTACGGTCTTCAGAGTCTCTCAAGCCAAAATTGGAGTGCCATTTTCGTTTCAATCTCAGGATTCTGCTTCTCGTTCATTCTCCCGGATTCAATCTCCACCTGAACGTTTCGTTTATTTATTTACTGGCAACCAAGAAGTGAAAAGAGCTTATGATGATCACAGTTCTTCTTTCGGGATTGCATTTGACATCGATGGGGTTGTTCTACGTGGCCACACTCCTATTGGAGGGTCTCCCAAAGCTTTGAGAAGGCTGTATGCGGATTCTACATCCTCTGGAGGAGGTACCCCAGAGTCCAGACGCTCCATTGAATTGAGTGACCTTTTAGGAGTGAATATATTACCTTCTCAGGTTGTACAGGGCCACTCAAGTTTTAAAAGTTTGTTGAGTAGGTTTGAGAATGAACTTATCGTTGCAACTGGAAAGGGTGAGCCAGCTCTTGTTATGTCTGAGTATGGTTTCAAAAAAGTTTTCTCCATTGATGAGTATGCTTCCTTCTTTGAGAATATTGATCCTGTATCCCAATACAAGAACTGGACAACCAAACAAGATTTTGATTTGACTAGCAACCCTGTGAAGTTGATGAGGAGAAGGCAGAGTGTTTTCTCTGAAAGAGTGAAAGCGGCTTTTGTTGTCAGTGATCCTGTTGATTGGGGCAGGGATATCCAAGTTCTTTGTGATATTCTAAGATCTGGAGGCCTTCCAGGACAGGAGAATGGGAATCAGGTGCCTCTATATTTTGCTGCAGATGATTTTGAATATCAGCACAGGGTTACATCTCCTAATGGTGCCGTGCAATTGTTTGTCATTTCTCTTACTCTGCATCTACCTAGCCTACCATTCTCTCCATTAGTTTTTAAGGCTACAAATGCCATTGCCAGTAAAATCGAGAATTTGTCGATTGGATTTTTTGTGCAGGCTGCATTTCCGTTTAAGCGGCTTGGGATAGGTGCCTTTAAAATTGCACTGGAAAGTATATTTAATAGGATTCACCATAGACCTCTCGAATATGTAACTCACGGGAAACCAAATCCTCTTGTGTTTAAGAATGTTGAAGTTCTGCTGAGACAAATTCTCTCATCTCACTGTGATGATCATTTTGTGAACAATCGAGATATGGAATTTACTCGTTTTAAAACACTTTATATGATCGGCGACAACCCCGCGGTAGACATCAAAGGTGCACGAGAGGCAGGACGACCTTGGTTTTCTATTTTAACTAGGACAGGAGTGTTCAGGGGCGAAGAAAATCACGCCGAGTTTCCTGCCGATCTTGTCGTTGATACCATTGAAGAAGCAGTGGACTATATTTTCGAAACTGAGTGCATTTCTTAA

Protein sequence

MKILTVFRVSQAKIGVPFSFQSQDSASRSFSRIQSPPERFVYLFTGNQEVKRAYDDHSSSFGIAFDIDGVVLRGHTPIGGSPKALRRLYADSTSSGGGTPESRRSIELSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQVLCDILRSGGLPGQENGNQVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFSPLVFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVTHGKPNPLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGAREAGRPWFSILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFETECIS
Homology
BLAST of MS025881 vs. NCBI nr
Match: XP_022993080.1 (uncharacterized protein YKR070W-like [Cucurbita maxima])

HSP 1 Score: 609.0 bits (1569), Expect = 3.3e-170
Identity = 326/460 (70.87%), Postives = 348/460 (75.65%), Query Frame = 0

Query: 1   MKILTVFRVSQAKIGVPFSFQSQDSAS-RSFSRIQSPPERFVYLFTGNQEVKRAYDDHSS 60
           MKILTVFRVSQ  I    SFQSQ SA   SFS IQS  ER                   S
Sbjct: 24  MKILTVFRVSQGNIR---SFQSQASAGFYSFSPIQSRLER-------------------S 83

Query: 61  SFGIAFDIDGVVLRGHTPIGGSPKALRRLYADSTSS------------GGGTPESRRSIE 120
           SFGIAFDIDGVVLRGH PIGGS KAL+RLYADSTSS            GGGTPESRR+IE
Sbjct: 84  SFGIAFDIDGVVLRGHRPIGGSSKALKRLYADSTSSGTLKVPFLFLTNGGGTPESRRAIE 143

Query: 121 LSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDEYA 180
           LS+LLGVN+LPSQVVQGHSSF+SLL+ FENELI+ATGKG+PALVMSEYGFKKVFS+DEYA
Sbjct: 144 LSELLGVNVLPSQVVQGHSSFRSLLNSFENELIIATGKGQPALVMSEYGFKKVFSLDEYA 203

Query: 181 SFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQV 240
           SFFENIDPVSQYKNWT++Q FDLT NP KLM R+QSV SERVKAAFVVSDPVDWGRDIQV
Sbjct: 204 SFFENIDPVSQYKNWTSQQAFDLTCNPEKLM-RKQSVLSERVKAAFVVSDPVDWGRDIQV 263

Query: 241 LCDILRSGGLPGQENGNQVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFSPL 300
           LCD+LRSGGLPGQENGNQVPLYFAADDFEY                              
Sbjct: 264 LCDVLRSGGLPGQENGNQVPLYFAADDFEY------------------------------ 323

Query: 301 VFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVTHGKPN 360
                                QAAFPFKRLGIGAFKIALESIFNRIHHRPLEYV++GKPN
Sbjct: 324 ---------------------QAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVSYGKPN 383

Query: 361 PLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGAREAGRPWFS 420
           PLVFKNVE +LRQILSSHCDDH VN  D+EF  F+TLYMIGDNP VDIKGAREAGRPWFS
Sbjct: 384 PLVFKNVETVLRQILSSHCDDHLVNKEDIEFNGFRTLYMIGDNPVVDIKGAREAGRPWFS 409

Query: 421 ILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFETECIS 448
           ILTRTGVFRGEENH EFPADLVV+T+EEAV+YI E+ECIS
Sbjct: 444 ILTRTGVFRGEENHVEFPADLVVNTVEEAVEYILESECIS 409

BLAST of MS025881 vs. NCBI nr
Match: XP_023549962.1 (uncharacterized protein YKR070W-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023549963.1 uncharacterized protein YKR070W-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 605.9 bits (1561), Expect = 2.8e-169
Identity = 326/460 (70.87%), Postives = 347/460 (75.43%), Query Frame = 0

Query: 1   MKILTVFRVSQAKIGVPFSFQSQDSAS-RSFSRIQSPPERFVYLFTGNQEVKRAYDDHSS 60
           MKILTVFRVSQ  I    SFQS  SA   SFS IQS  ER                   S
Sbjct: 1   MKILTVFRVSQGTIR---SFQSLASAGFYSFSPIQSRLER-------------------S 60

Query: 61  SFGIAFDIDGVVLRGHTPIGGSPKALRRLYADSTSS------------GGGTPESRRSIE 120
           SFGIAFDIDGVVLRGH PIGGS KAL+RLYADSTSS            GGGTPESRR+IE
Sbjct: 61  SFGIAFDIDGVVLRGHRPIGGSSKALKRLYADSTSSGTLKVPFLFLTNGGGTPESRRAIE 120

Query: 121 LSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDEYA 180
           LS+LLGVN+LPSQVVQGHSSF+SLL+ FENELI+ATGKG+PALVMSEYGFKKVFS+DEYA
Sbjct: 121 LSELLGVNVLPSQVVQGHSSFRSLLNSFENELIIATGKGQPALVMSEYGFKKVFSLDEYA 180

Query: 181 SFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQV 240
           SFFENIDPVSQYKNWT++Q FDLT NP KLM R+QSV SERVKAAFVVSDPVDWGRDIQV
Sbjct: 181 SFFENIDPVSQYKNWTSQQAFDLTCNPEKLM-RKQSVLSERVKAAFVVSDPVDWGRDIQV 240

Query: 241 LCDILRSGGLPGQENGNQVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFSPL 300
           LCD+LRSGGLPGQENGNQVPLYFAADDFEY                              
Sbjct: 241 LCDVLRSGGLPGQENGNQVPLYFAADDFEY------------------------------ 300

Query: 301 VFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVTHGKPN 360
                                QAAFPFKRLGIGAFKIALESIFNRIHHRPLEYV++GKPN
Sbjct: 301 ---------------------QAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVSYGKPN 360

Query: 361 PLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGAREAGRPWFS 420
           PLVFKNVE +LRQILSSHCDDHFVN  D+EF  FKTLYMIGDNP VDIKGAREAGRPWFS
Sbjct: 361 PLVFKNVETVLRQILSSHCDDHFVNKEDIEFNSFKTLYMIGDNPVVDIKGAREAGRPWFS 386

Query: 421 ILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFETECIS 448
           ILTRTGVFRGEEN  EFPADLVV+T+EEAV+YI E+ECIS
Sbjct: 421 ILTRTGVFRGEENQDEFPADLVVNTVEEAVEYILESECIS 386

BLAST of MS025881 vs. NCBI nr
Match: XP_022938837.1 (uncharacterized protein YKR070W-like [Cucurbita moschata])

HSP 1 Score: 604.0 bits (1556), Expect = 1.1e-168
Identity = 323/460 (70.22%), Postives = 346/460 (75.22%), Query Frame = 0

Query: 1   MKILTVFRVSQAKIGVPFSFQSQDSAS-RSFSRIQSPPERFVYLFTGNQEVKRAYDDHSS 60
           MKILTVFRVSQ  I    SFQSQ SA   SFS IQS  ER                   S
Sbjct: 24  MKILTVFRVSQGNIR---SFQSQASAGFYSFSPIQSRLER-------------------S 83

Query: 61  SFGIAFDIDGVVLRGHTPIGGSPKALRRLYADSTSS------------GGGTPESRRSIE 120
           SFGIAFDIDGVVLRGH PIGGS KAL+RLYADSTSS            GGGTPES+R+IE
Sbjct: 84  SFGIAFDIDGVVLRGHRPIGGSSKALKRLYADSTSSGTLKVPFLFLTNGGGTPESKRAIE 143

Query: 121 LSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDEYA 180
           LS+LLGVN+LPSQVVQGHSSF+SLL+ FENELI+ATGKG+PALVMSEYGFKKVFS+DEYA
Sbjct: 144 LSELLGVNVLPSQVVQGHSSFRSLLNSFENELIIATGKGQPALVMSEYGFKKVFSLDEYA 203

Query: 181 SFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQV 240
           SFFENIDPVSQYKNWT++Q FD T NP KLM R+QSV SERVKAAFVVSDPVDWGRDIQV
Sbjct: 204 SFFENIDPVSQYKNWTSQQAFDSTCNPEKLM-RKQSVLSERVKAAFVVSDPVDWGRDIQV 263

Query: 241 LCDILRSGGLPGQENGNQVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFSPL 300
           LCD+LRSGGLPGQENGNQVPLYFAADDFEY                              
Sbjct: 264 LCDVLRSGGLPGQENGNQVPLYFAADDFEY------------------------------ 323

Query: 301 VFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVTHGKPN 360
                                QAAFP KRLGIGAFKIALESIFNRIHHRPLEYV++GKPN
Sbjct: 324 ---------------------QAAFPLKRLGIGAFKIALESIFNRIHHRPLEYVSYGKPN 383

Query: 361 PLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGAREAGRPWFS 420
           PLVFKNVE +LRQILSSHCDDHFVN  D+EF  F+T YMIGDNP VDIKGAREAGRPWFS
Sbjct: 384 PLVFKNVETVLRQILSSHCDDHFVNKEDVEFNGFRTFYMIGDNPVVDIKGAREAGRPWFS 409

Query: 421 ILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFETECIS 448
           ILTRTGVFRGEENH EFPADLVV+T+EEAV+YI E+ECIS
Sbjct: 444 ILTRTGVFRGEENHVEFPADLVVNTVEEAVEYILESECIS 409

BLAST of MS025881 vs. NCBI nr
Match: KAG6578738.1 (hypothetical protein SDJN03_23186, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 603.2 bits (1554), Expect = 1.8e-168
Identity = 322/460 (70.00%), Postives = 347/460 (75.43%), Query Frame = 0

Query: 1   MKILTVFRVSQAKIGVPFSFQSQDSAS-RSFSRIQSPPERFVYLFTGNQEVKRAYDDHSS 60
           MKILTVFRVSQ  I    SFQSQ SA   SFS IQS  ER                   S
Sbjct: 24  MKILTVFRVSQGNIR---SFQSQASAGFYSFSPIQSRLER-------------------S 83

Query: 61  SFGIAFDIDGVVLRGHTPIGGSPKALRRLYADSTSS------------GGGTPESRRSIE 120
           SFGIAFDIDGVVLRGH PIGGS KAL+RLYADSTSS            GGGTPES+R+IE
Sbjct: 84  SFGIAFDIDGVVLRGHRPIGGSSKALKRLYADSTSSGTLKVPFLFLTNGGGTPESKRAIE 143

Query: 121 LSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDEYA 180
           LS+LLGVN+LPSQVVQGHSSF+SLL+ FENELI+ATGKG+PALVMSEYGFKKVFS+DEYA
Sbjct: 144 LSELLGVNVLPSQVVQGHSSFRSLLNSFENELIIATGKGQPALVMSEYGFKKVFSLDEYA 203

Query: 181 SFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQV 240
           SFFENIDPVSQYKNWT++Q FD T NP KLM R+QSV SERVKAAFVVSDPVDWGRDIQV
Sbjct: 204 SFFENIDPVSQYKNWTSQQAFDSTCNPEKLM-RKQSVLSERVKAAFVVSDPVDWGRDIQV 263

Query: 241 LCDILRSGGLPGQENGNQVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFSPL 300
           LCD+LRSGGLPGQENGNQVPLYF+ADDFEY                              
Sbjct: 264 LCDVLRSGGLPGQENGNQVPLYFSADDFEY------------------------------ 323

Query: 301 VFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVTHGKPN 360
                                QAAFP KRLGIGAFKIALESIFNRIHH+PLEYV++GKPN
Sbjct: 324 ---------------------QAAFPLKRLGIGAFKIALESIFNRIHHKPLEYVSYGKPN 383

Query: 361 PLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGAREAGRPWFS 420
           PLVFKNVE +LRQILSSHCDDHFVN  D+EF  F+TLYMIGDNP VDIKGAREAGRPWFS
Sbjct: 384 PLVFKNVETVLRQILSSHCDDHFVNKEDVEFNGFRTLYMIGDNPVVDIKGAREAGRPWFS 409

Query: 421 ILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFETECIS 448
           ILTRTGVFRGEENH EFPADLVV+T+EEAV+YI E+ECIS
Sbjct: 444 ILTRTGVFRGEENHVEFPADLVVNTVEEAVEYILESECIS 409

BLAST of MS025881 vs. NCBI nr
Match: KAG7016268.1 (hypothetical protein SDJN02_21374 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 602.8 bits (1553), Expect = 2.4e-168
Identity = 322/460 (70.00%), Postives = 346/460 (75.22%), Query Frame = 0

Query: 1   MKILTVFRVSQAKIGVPFSFQSQDSAS-RSFSRIQSPPERFVYLFTGNQEVKRAYDDHSS 60
           MKILTVFRVSQ  I    SFQSQ SA   SFS IQS  ER                   S
Sbjct: 24  MKILTVFRVSQGNIR---SFQSQASAGFYSFSPIQSRLER-------------------S 83

Query: 61  SFGIAFDIDGVVLRGHTPIGGSPKALRRLYADSTSS------------GGGTPESRRSIE 120
           SFGIAFDIDGVVLRGH PIGGS KAL+RLYADSTSS            GGGTPES+R+IE
Sbjct: 84  SFGIAFDIDGVVLRGHRPIGGSSKALKRLYADSTSSGTLKVPFLFLTNGGGTPESKRAIE 143

Query: 121 LSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDEYA 180
           LS+LLGVN+LPSQVVQGHSSF+SLL+ FENELI+ATGKG+PALVMSEYGFKKVFS+DEYA
Sbjct: 144 LSELLGVNVLPSQVVQGHSSFRSLLNSFENELIIATGKGQPALVMSEYGFKKVFSLDEYA 203

Query: 181 SFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQV 240
           SFFENIDPVSQYKNWT++Q FD T NP KLM R+Q V SERVKAAFVVSDPVDWGRDIQV
Sbjct: 204 SFFENIDPVSQYKNWTSQQAFDSTCNPEKLM-RKQGVLSERVKAAFVVSDPVDWGRDIQV 263

Query: 241 LCDILRSGGLPGQENGNQVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFSPL 300
           LCD+LRSGGLPGQENGNQVPLYF+ADDFEY                              
Sbjct: 264 LCDVLRSGGLPGQENGNQVPLYFSADDFEY------------------------------ 323

Query: 301 VFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVTHGKPN 360
                                QAAFP KRLGIGAFKIALESIFNRIHHRPLEYV++GKPN
Sbjct: 324 ---------------------QAAFPLKRLGIGAFKIALESIFNRIHHRPLEYVSYGKPN 383

Query: 361 PLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGAREAGRPWFS 420
           PLVFKNVE +LRQILSSHCDDHFVN  D+EF  F+TLYMIGDNP VDIKGAREAGRPWFS
Sbjct: 384 PLVFKNVETVLRQILSSHCDDHFVNKEDVEFNGFRTLYMIGDNPVVDIKGAREAGRPWFS 409

Query: 421 ILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFETECIS 448
           ILTRTGVFRGEENH EFPADLVV+T+EEAV+YI E+ECIS
Sbjct: 444 ILTRTGVFRGEENHVEFPADLVVNTVEEAVEYILESECIS 409

BLAST of MS025881 vs. ExPASy Swiss-Prot
Match: P36151 (Uncharacterized protein YKR070W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YKR070W PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.2e-28
Identity = 102/411 (24.82%), Postives = 172/411 (41.85%), Query Frame = 0

Query: 51  KRAYDDHSSSFGIAFDIDGVVLRGHTPIGGSPKALRRLYADS-----TSSGGGTPESRRS 110
           KR +   S     AFDIDGV+ RG  PI G+  AL+ L  +       ++GGG  E  R+
Sbjct: 4   KRFFQTTSKKIAFAFDIDGVLFRGKKPIAGASDALKLLNRNKIPYILLTNGGGFSERART 63

Query: 111 IELSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDE 170
             +S  L V++ P Q++Q H+ +KSL++++    I+A G      V   YGF+ V    +
Sbjct: 64  EFISSKLDVDVSPLQIIQSHTPYKSLVNKYSR--ILAVGTPSVRGVAEGYGFQDVVHQTD 123

Query: 171 YASFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDI 230
              +  +I P S   +             ++  R    + +++  A  V +DP DW  DI
Sbjct: 124 IVRYNRDIAPFSGLSD----------EQVMEYSRDIPDLTTKKFDAVLVFNDPHDWAADI 183

Query: 231 QVLCDILRS-----GGLPGQENGN-QVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHL 290
           Q++ D + S       L  +++G   +P+YF+  D  + +                    
Sbjct: 184 QIISDAINSENGMLNTLRNEKSGKPSIPIYFSNQDLLWAN-------------------- 243

Query: 291 PSLPFSPLVFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLE 350
                                           +   R G GAF++ +  ++  ++  PL+
Sbjct: 244 -------------------------------PYKLNRFGQGAFRLLVRRLYLELNGEPLQ 303

Query: 351 YVTHGKPNPLVF---KNVEVLLRQILSSHCDDHFVNNRDMEFTR-----FKTLYMIGDNP 410
             T GKP  L +    +V +   + LS            +  T+     F  ++M+GDNP
Sbjct: 304 DYTLGKPTKLTYDFAHHVLIDWEKRLSGKIGQSVKQKLPLLGTKPSTSPFHAVFMVGDNP 349

Query: 411 AVDIKGAREAGRPWFSILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFE 443
           A DI GA+  G  W S L +TGV+   ++  E    L+V+ + +AV    E
Sbjct: 364 ASDIIGAQNYG--WNSCLVKTGVYNEGDDLKECKPTLIVNDVFDAVTKTLE 349

BLAST of MS025881 vs. ExPASy Swiss-Prot
Match: O13899 (Uncharacterized CDP-alcohol phosphatidyltransferase class-I family protein C22A12.08c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC22A12.08c PE=3 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 4.4e-24
Identity = 103/387 (26.61%), Postives = 164/387 (42.38%), Query Frame = 0

Query: 64  AFD-IDGVVLRGHTPIGGSPKALRRLYADS-----TSSGGGTPESRRSIELSDLLGVNIL 123
           AFD IDGV++RG   +    K L+ L  ++      ++GGG  ES R+  LS  L V++ 
Sbjct: 41  AFDSIDGVLIRGGRGLKEGTKTLKFLQKNNIPFILLTNGGGMHESVRAQRLSKTLSVSLT 100

Query: 124 PSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFS-IDEYASFFENIDPV 183
                Q H+ F++L  ++++ L++            +YGFK V + +D  A       P 
Sbjct: 101 EDDFCQSHTPFRALADKYKHVLVLGGKDNSVRETAEKYGFKSVINELDVIAKLGTPFWPF 160

Query: 184 SQYKNWTTK--QDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQVLCDILRS 243
           + +     K  +DFD+T                 ++A F   DPV  G D+Q++ ++ +S
Sbjct: 161 TSFNEEDIKDAKDFDVT---------------RPIEAVFTYVDPVRLGLDLQLVMELGQS 220

Query: 244 -GGLPG--QENGNQVP-LYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFSPLVFK 303
             G+ G   +  N+ P +YF+  D  +      PN                         
Sbjct: 221 KNGVLGTVSKTANEGPDIYFSNADLIW------PN------------------------- 280

Query: 304 ATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVTHGKPNPLV 363
                                +P  RLG GAF I  ES+F  +  + L    +GKP+ L 
Sbjct: 281 --------------------EYPLPRLGQGAFAICCESVFKELTGKDLRNTKYGKPHKLT 340

Query: 364 FKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGAREAGRPWFSILT 423
           +   + +L   +  H      N         K ++M+GDNP  DI+GA   G  W SIL 
Sbjct: 341 YDYAKNIL---MKKHKTLGITNPP-------KEIFMVGDNPESDIRGANNYG--WTSILV 348

Query: 424 RTGVFRGEENHAEFPADLVVDTIEEAV 438
           RTG+F+G +N  ++ A  V D + E V
Sbjct: 401 RTGIFQG-DNSPKYSAKHVSDNVWEGV 348

BLAST of MS025881 vs. ExPASy Swiss-Prot
Match: Q91WM2 (Haloacid dehalogenase-like hydrolase domain-containing 5 OS=Mus musculus OX=10090 GN=Hdhd5 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.6e-21
Identity = 95/353 (26.91%), Postives = 145/353 (41.08%), Query Frame = 0

Query: 56  DHSSSFGIAFDIDGVVLRGHTPIGGSPKALRRLYADS---------TSSGGGTPESRRSI 115
           + S +FG+ FDIDGV++RGH  I  + +A  +L              ++ G   +  ++ 
Sbjct: 42  ERSPTFGLLFDIDGVLVRGHRVIPAALEAFSKLVNSQGQLRVPVVFVTNAGNILQHNKAQ 101

Query: 116 ELSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDEY 175
           ELSDLL   + P QV+  HS  K  L     +++V +G+G         GF+ V +IDE 
Sbjct: 102 ELSDLLRCKVDPDQVILSHSPMKLFLQYHSKQMLV-SGQGPLVENARALGFQNVVTIDEL 161

Query: 176 ASFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQ 235
              F  +D V            DL   P K MR R       ++   ++ +PV W  ++Q
Sbjct: 162 RLAFPELDMV------------DLQRRP-KTMRLRSDF--PAIEGVLLLGEPVRWETNLQ 221

Query: 236 VLCDILRSGGLPGQENGNQVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFSP 295
           ++ D+L S G PG                      T+P             HLP L    
Sbjct: 222 LIMDVLLSNGHPG------------------TGLATAPYP-----------HLPVL---- 281

Query: 296 LVFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYV-THGK 355
                         N+ + +  +A  P  R G G F + LE+I+ +I    L+Y    GK
Sbjct: 282 ------------ASNMDLLWMAEAKMP--RFGHGTFLLCLETIYRKITGNELKYEGLMGK 321

Query: 356 PNPLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGA 399
           P+ L ++  E ++RQ             R       + LY IGDNP  D+ GA
Sbjct: 342 PSILTYQYAEDVIRQ----------QAERRGWAAPIRKLYAIGDNPMSDVYGA 321

BLAST of MS025881 vs. ExPASy Swiss-Prot
Match: Q9BXW7 (Haloacid dehalogenase-like hydrolase domain-containing 5 OS=Homo sapiens OX=9606 GN=HDHD5 PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 2.9e-20
Identity = 97/387 (25.06%), Postives = 151/387 (39.02%), Query Frame = 0

Query: 26  ASRSFSRIQSPPERFVYLFTGNQEVKRAYDDHSSSFGIAFDIDGVVLRGHTPIGGSPKAL 85
           A+R+ + +Q  P R  Y     Q           +FG   DIDGV++RGH  I  + KA 
Sbjct: 20  AARAAAGLQGRPARRCYAVGPAQS--------PPTFGFLLDIDGVLVRGHRVIPAALKAF 79

Query: 86  RRLYADS---------TSSGGGTPESRRSIELSDLLGVNILPSQVVQGHSSFKSLLSRFE 145
           RRL              ++ G   +  ++ ELS LLG  +   QV+  HS  K L S + 
Sbjct: 80  RRLVNSQGQLRVPVVFVTNAGNILQHSKAQELSALLGCEVDADQVILSHSPMK-LFSEYH 139

Query: 146 NELIVATGKGEPALVMSEYGFKKVFSIDEYASFFENIDPVSQYKNWTT----KQDFDLTS 205
            + ++ +G+G         GF+ V ++DE    F  +D V   +   T    + DF    
Sbjct: 140 EKRMLVSGQGPVMENAQGLGFRNVVTVDELRMAFPLLDMVDLERRLKTTPLPRNDF---- 199

Query: 206 NPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQVLCDILRSGGLPGQENGNQVPLYFAA 265
                          R++   ++ +PV W   +Q++ D+L S G PG   G   P Y   
Sbjct: 200 --------------PRIEGVLLLGEPVRWETSLQLIMDVLLSNGSPGA--GLATPPY--- 259

Query: 266 DDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFSPLVFKATNAIASKIENLSIGFFVQAAF 325
                                    HLP L                  N+ + +  +A  
Sbjct: 260 ------------------------PHLPVL----------------ASNMDLLWMAEAKM 319

Query: 326 PFKRLGIGAFKIALESIFNRIHHRPLEYV-THGKPNPLVFKNVEVLLRQILSSHCDDHFV 385
           P  R G G F + LE+I+ ++  + L Y    GKP+ L ++  E L+R+           
Sbjct: 320 P--RFGHGTFLLCLETIYQKVTGKELRYEGLMGKPSILTYQYAEDLIRR----------Q 322

Query: 386 NNRDMEFTRFKTLYMIGDNPAVDIKGA 399
             R       + LY +GDNP  D+ GA
Sbjct: 380 AERRGWAAPIRKLYAVGDNPMSDVYGA 322

BLAST of MS025881 vs. ExPASy TrEMBL
Match: A0A6J1JXI7 (uncharacterized protein YKR070W-like OS=Cucurbita maxima OX=3661 GN=LOC111489204 PE=4 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.6e-170
Identity = 326/460 (70.87%), Postives = 348/460 (75.65%), Query Frame = 0

Query: 1   MKILTVFRVSQAKIGVPFSFQSQDSAS-RSFSRIQSPPERFVYLFTGNQEVKRAYDDHSS 60
           MKILTVFRVSQ  I    SFQSQ SA   SFS IQS  ER                   S
Sbjct: 24  MKILTVFRVSQGNIR---SFQSQASAGFYSFSPIQSRLER-------------------S 83

Query: 61  SFGIAFDIDGVVLRGHTPIGGSPKALRRLYADSTSS------------GGGTPESRRSIE 120
           SFGIAFDIDGVVLRGH PIGGS KAL+RLYADSTSS            GGGTPESRR+IE
Sbjct: 84  SFGIAFDIDGVVLRGHRPIGGSSKALKRLYADSTSSGTLKVPFLFLTNGGGTPESRRAIE 143

Query: 121 LSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDEYA 180
           LS+LLGVN+LPSQVVQGHSSF+SLL+ FENELI+ATGKG+PALVMSEYGFKKVFS+DEYA
Sbjct: 144 LSELLGVNVLPSQVVQGHSSFRSLLNSFENELIIATGKGQPALVMSEYGFKKVFSLDEYA 203

Query: 181 SFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQV 240
           SFFENIDPVSQYKNWT++Q FDLT NP KLM R+QSV SERVKAAFVVSDPVDWGRDIQV
Sbjct: 204 SFFENIDPVSQYKNWTSQQAFDLTCNPEKLM-RKQSVLSERVKAAFVVSDPVDWGRDIQV 263

Query: 241 LCDILRSGGLPGQENGNQVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFSPL 300
           LCD+LRSGGLPGQENGNQVPLYFAADDFEY                              
Sbjct: 264 LCDVLRSGGLPGQENGNQVPLYFAADDFEY------------------------------ 323

Query: 301 VFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVTHGKPN 360
                                QAAFPFKRLGIGAFKIALESIFNRIHHRPLEYV++GKPN
Sbjct: 324 ---------------------QAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVSYGKPN 383

Query: 361 PLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGAREAGRPWFS 420
           PLVFKNVE +LRQILSSHCDDH VN  D+EF  F+TLYMIGDNP VDIKGAREAGRPWFS
Sbjct: 384 PLVFKNVETVLRQILSSHCDDHLVNKEDIEFNGFRTLYMIGDNPVVDIKGAREAGRPWFS 409

Query: 421 ILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFETECIS 448
           ILTRTGVFRGEENH EFPADLVV+T+EEAV+YI E+ECIS
Sbjct: 444 ILTRTGVFRGEENHVEFPADLVVNTVEEAVEYILESECIS 409

BLAST of MS025881 vs. ExPASy TrEMBL
Match: A0A6J1FF73 (uncharacterized protein YKR070W-like OS=Cucurbita moschata OX=3662 GN=LOC111444927 PE=4 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 5.1e-169
Identity = 323/460 (70.22%), Postives = 346/460 (75.22%), Query Frame = 0

Query: 1   MKILTVFRVSQAKIGVPFSFQSQDSAS-RSFSRIQSPPERFVYLFTGNQEVKRAYDDHSS 60
           MKILTVFRVSQ  I    SFQSQ SA   SFS IQS  ER                   S
Sbjct: 24  MKILTVFRVSQGNIR---SFQSQASAGFYSFSPIQSRLER-------------------S 83

Query: 61  SFGIAFDIDGVVLRGHTPIGGSPKALRRLYADSTSS------------GGGTPESRRSIE 120
           SFGIAFDIDGVVLRGH PIGGS KAL+RLYADSTSS            GGGTPES+R+IE
Sbjct: 84  SFGIAFDIDGVVLRGHRPIGGSSKALKRLYADSTSSGTLKVPFLFLTNGGGTPESKRAIE 143

Query: 121 LSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDEYA 180
           LS+LLGVN+LPSQVVQGHSSF+SLL+ FENELI+ATGKG+PALVMSEYGFKKVFS+DEYA
Sbjct: 144 LSELLGVNVLPSQVVQGHSSFRSLLNSFENELIIATGKGQPALVMSEYGFKKVFSLDEYA 203

Query: 181 SFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQV 240
           SFFENIDPVSQYKNWT++Q FD T NP KLM R+QSV SERVKAAFVVSDPVDWGRDIQV
Sbjct: 204 SFFENIDPVSQYKNWTSQQAFDSTCNPEKLM-RKQSVLSERVKAAFVVSDPVDWGRDIQV 263

Query: 241 LCDILRSGGLPGQENGNQVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFSPL 300
           LCD+LRSGGLPGQENGNQVPLYFAADDFEY                              
Sbjct: 264 LCDVLRSGGLPGQENGNQVPLYFAADDFEY------------------------------ 323

Query: 301 VFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVTHGKPN 360
                                QAAFP KRLGIGAFKIALESIFNRIHHRPLEYV++GKPN
Sbjct: 324 ---------------------QAAFPLKRLGIGAFKIALESIFNRIHHRPLEYVSYGKPN 383

Query: 361 PLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGAREAGRPWFS 420
           PLVFKNVE +LRQILSSHCDDHFVN  D+EF  F+T YMIGDNP VDIKGAREAGRPWFS
Sbjct: 384 PLVFKNVETVLRQILSSHCDDHFVNKEDVEFNGFRTFYMIGDNPVVDIKGAREAGRPWFS 409

Query: 421 ILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFETECIS 448
           ILTRTGVFRGEENH EFPADLVV+T+EEAV+YI E+ECIS
Sbjct: 444 ILTRTGVFRGEENHVEFPADLVVNTVEEAVEYILESECIS 409

BLAST of MS025881 vs. ExPASy TrEMBL
Match: A0A5D3C5R2 (Hydrolase family protein / HAD-superfamily protein isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G002260 PE=4 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 5.3e-150
Identity = 282/402 (70.15%), Postives = 304/402 (75.62%), Query Frame = 0

Query: 60  SFGIAFDIDGVVLRGHTPIGGSPKALRRLYADST------------SSGGGTPESRRSIE 119
           SFGIAFDIDGV+LRG  PIGGS KALRRLY DST            ++GGGTPESRR+IE
Sbjct: 200 SFGIAFDIDGVLLRGQHPIGGSTKALRRLYVDSTFSGTLKVPFLFLTNGGGTPESRRAIE 259

Query: 120 LSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDEYA 179
           LS+LLGVN+LPSQVVQGHSSFKSLL+RFENELI+ATGKG+P LVMSEYGFKKVFSI EYA
Sbjct: 260 LSELLGVNVLPSQVVQGHSSFKSLLNRFENELIIATGKGQPDLVMSEYGFKKVFSIGEYA 319

Query: 180 SFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQV 239
           SFFENIDPVSQYK+WT+KQ FD   +P   M RRQSV SERVKAAFVVSDPVDWGRDIQV
Sbjct: 320 SFFENIDPVSQYKSWTSKQAFDSNCDPHGSM-RRQSVLSERVKAAFVVSDPVDWGRDIQV 379

Query: 240 LCDILRSGGLPGQENG--NQVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFS 299
           LCD+LRSGGLPG +NG  NQVPLYFAADD EY                            
Sbjct: 380 LCDVLRSGGLPGHQNGNLNQVPLYFAADDLEY---------------------------- 439

Query: 300 PLVFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVTHGK 359
                                  Q AFPFKRLGIGAFKIALESIF RIHH PLEYV +GK
Sbjct: 440 -----------------------QGAFPFKRLGIGAFKIALESIFERIHHIPLEYVCYGK 499

Query: 360 PNPLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGAREAGRPW 419
           PNPLVFKNVE + RQIL SHCDDHFVN  D+E   FKTLYMIGDNP VDIKGAREAGRPW
Sbjct: 500 PNPLVFKNVEAVFRQILLSHCDDHFVNKGDIEVNGFKTLYMIGDNPVVDIKGAREAGRPW 549

Query: 420 FSILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFETECIS 448
           FSILTRTGVF+G+ENH EFPADLVV+T+EEAVDYIF +E IS
Sbjct: 560 FSILTRTGVFKGKENHVEFPADLVVNTVEEAVDYIFASEHIS 549

BLAST of MS025881 vs. ExPASy TrEMBL
Match: A0A1S3CK22 (uncharacterized protein YKR070W-like OS=Cucumis melo OX=3656 GN=LOC103501676 PE=4 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 5.3e-150
Identity = 282/402 (70.15%), Postives = 304/402 (75.62%), Query Frame = 0

Query: 60  SFGIAFDIDGVVLRGHTPIGGSPKALRRLYADST------------SSGGGTPESRRSIE 119
           SFGIAFDIDGV+LRG  PIGGS KALRRLY DST            ++GGGTPESRR+IE
Sbjct: 30  SFGIAFDIDGVLLRGQHPIGGSTKALRRLYVDSTFSGTLKVPFLFLTNGGGTPESRRAIE 89

Query: 120 LSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDEYA 179
           LS+LLGVN+LPSQVVQGHSSFKSLL+RFENELI+ATGKG+P LVMSEYGFKKVFSI EYA
Sbjct: 90  LSELLGVNVLPSQVVQGHSSFKSLLNRFENELIIATGKGQPDLVMSEYGFKKVFSIGEYA 149

Query: 180 SFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDIQV 239
           SFFENIDPVSQYK+WT+KQ FD   +P   M RRQSV SERVKAAFVVSDPVDWGRDIQV
Sbjct: 150 SFFENIDPVSQYKSWTSKQAFDSNCDPHGSM-RRQSVLSERVKAAFVVSDPVDWGRDIQV 209

Query: 240 LCDILRSGGLPGQENG--NQVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFS 299
           LCD+LRSGGLPG +NG  NQVPLYFAADD EY                            
Sbjct: 210 LCDVLRSGGLPGHQNGNLNQVPLYFAADDLEY---------------------------- 269

Query: 300 PLVFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVTHGK 359
                                  Q AFPFKRLGIGAFKIALESIF RIHH PLEYV +GK
Sbjct: 270 -----------------------QGAFPFKRLGIGAFKIALESIFERIHHIPLEYVCYGK 329

Query: 360 PNPLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGAREAGRPW 419
           PNPLVFKNVE + RQIL SHCDDHFVN  D+E   FKTLYMIGDNP VDIKGAREAGRPW
Sbjct: 330 PNPLVFKNVEAVFRQILLSHCDDHFVNKGDIEVNGFKTLYMIGDNPVVDIKGAREAGRPW 379

Query: 420 FSILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFETECIS 448
           FSILTRTGVF+G+ENH EFPADLVV+T+EEAVDYIF +E IS
Sbjct: 390 FSILTRTGVFKGKENHVEFPADLVVNTVEEAVDYIFASEHIS 379

BLAST of MS025881 vs. ExPASy TrEMBL
Match: A0A0A0KU31 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G497050 PE=4 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.9e-148
Identity = 281/419 (67.06%), Postives = 307/419 (73.27%), Query Frame = 0

Query: 43  LFTGNQEVKRAYDDHSSSFGIAFDIDGVVLRGHTPIGGSPKALRRLYADST--------- 102
           L T N  ++        SFGIAFDIDGV+LRG  PIGGS KALRRLY DST         
Sbjct: 15  LSTTNSRIQCRLLQPRPSFGIAFDIDGVLLRGQHPIGGSAKALRRLYVDSTFSGTLKVPF 74

Query: 103 ---SSGGGTPESRRSIELSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPAL 162
              ++GGGTPESRR+IELS+LLGVN+LPSQVVQGHSSFKSLL+ FENELI+ATGKG+P L
Sbjct: 75  LFLTNGGGTPESRRAIELSELLGVNVLPSQVVQGHSSFKSLLNSFENELIIATGKGQPDL 134

Query: 163 VMSEYGFKKVFSIDEYASFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVK 222
           VMSEYGFKKVFSI EYASFFENIDPVS YK+WT+KQ F+   NP +LM RRQSV SERVK
Sbjct: 135 VMSEYGFKKVFSIGEYASFFENIDPVSHYKSWTSKQAFNSNCNPHELM-RRQSVLSERVK 194

Query: 223 AAFVVSDPVDWGRDIQVLCDILRSGGLPGQENG--NQVPLYFAADDFEYQHRVTSPNGAV 282
           AAFVVSDPVDWGRDIQVLCD+LRSGGLPG +NG  NQVPLYFAADD EY           
Sbjct: 195 AAFVVSDPVDWGRDIQVLCDVLRSGGLPGYQNGNLNQVPLYFAADDLEY----------- 254

Query: 283 QLFVISLTLHLPSLPFSPLVFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALES 342
                                                   Q AFP KRLGIGAFKIALES
Sbjct: 255 ----------------------------------------QGAFPLKRLGIGAFKIALES 314

Query: 343 IFNRIHHRPLEYVTHGKPNPLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIG 402
           IFNRIHH PLEYV +GKPNPLVFKNVE + +QIL SHCDDHFVN  D+E   FK LYMIG
Sbjct: 315 IFNRIHHIPLEYVCYGKPNPLVFKNVEAVFKQILLSHCDDHFVNKGDIEVNGFKKLYMIG 374

Query: 403 DNPAVDIKGAREAGRPWFSILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFETECIS 448
           DNP VDIKGAREAGRPWFSILTRTGVF+ +ENH EFPADLVV+T+EEAVDYIF +E IS
Sbjct: 375 DNPVVDIKGAREAGRPWFSILTRTGVFKDKENHIEFPADLVVNTVEEAVDYIFASEHIS 381

BLAST of MS025881 vs. TAIR 10
Match: AT3G45740.1 (hydrolase family protein / HAD-superfamily protein )

HSP 1 Score: 411.8 bits (1057), Expect = 7.2e-115
Identity = 215/399 (53.88%), Postives = 266/399 (66.67%), Query Frame = 0

Query: 55  DDHSSSFGIAFDIDGVVLRGHTPIGGSPKALRRLYADS---------TSSGGGTPESRRS 114
           + + SSFGIAFDIDGV+L G +P+GGSP ALRRLY DS          ++GGG PES+R+
Sbjct: 33  ESNRSSFGIAFDIDGVILLGSSPVGGSPSALRRLYDDSGALKIPFLFLTNGGGLPESKRA 92

Query: 115 IELSDLLGVNILPSQVVQGHSSFKSLLSRFENELIVATGKGEPALVMSEYGFKKVFSIDE 174
            E+S LLGV + P QV+Q HS F+ L++RFENEL+VA GKGEPA VMS YGFK V S+DE
Sbjct: 93  SEMSHLLGVQVSPLQVIQAHSPFRKLVNRFENELVVAAGKGEPAAVMSNYGFKNVISMDE 152

Query: 175 YASFFENIDPVSQYKNWTTKQDFDLTSNPVKLMRRRQSVFSERVKAAFVVSDPVDWGRDI 234
           YAS+F+NIDP++ YK    +QD        K +R R+ V S+RV+AAF+VSDPVDW RDI
Sbjct: 153 YASYFDNIDPLAPYKKLMFRQDGH------KELRSREDVLSQRVQAAFIVSDPVDWSRDI 212

Query: 235 QVLCDILRSGGLPGQENGNQVPLYFAADDFEYQHRVTSPNGAVQLFVISLTLHLPSLPFS 294
           QVLCDILR+GGLPG+E G Q  LY A DD +Y                            
Sbjct: 213 QVLCDILRTGGLPGKEIGPQPHLYIANDDLDY---------------------------- 272

Query: 295 PLVFKATNAIASKIENLSIGFFVQAAFPFKRLGIGAFKIALESIFNRIHHRPLEYVTHGK 354
                                  Q  FP +RLG+GAF+IALESIFNRIH +PLEY + GK
Sbjct: 273 -----------------------QTEFPTERLGMGAFRIALESIFNRIHEKPLEYTSFGK 332

Query: 355 PNPLVFKNVEVLLRQILSSHCDDHFVNNRDMEFTRFKTLYMIGDNPAVDIKGAREAGRPW 414
           PNP VFKN E +L++I +S     + +N+      FKTLYMIGDNP +DI+GAR+AG PW
Sbjct: 333 PNPFVFKNAEDVLKEIATS----PYSSNQGSH--HFKTLYMIGDNPKIDIRGARQAGTPW 368

Query: 415 FSILTRTGVFRGEENHAEFPADLVVDTIEEAVDYIFETE 445
           FSILTRTGVF+G++NH+EFPADLVVDT+EEAVD+I   E
Sbjct: 393 FSILTRTGVFKGKDNHSEFPADLVVDTVEEAVDFILTRE 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022993080.13.3e-17070.87uncharacterized protein YKR070W-like [Cucurbita maxima][more]
XP_023549962.12.8e-16970.87uncharacterized protein YKR070W-like isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
XP_022938837.11.1e-16870.22uncharacterized protein YKR070W-like [Cucurbita moschata][more]
KAG6578738.11.8e-16870.00hypothetical protein SDJN03_23186, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7016268.12.4e-16870.00hypothetical protein SDJN02_21374 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
P361512.2e-2824.82Uncharacterized protein YKR070W OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
O138994.4e-2426.61Uncharacterized CDP-alcohol phosphatidyltransferase class-I family protein C22A1... [more]
Q91WM21.6e-2126.91Haloacid dehalogenase-like hydrolase domain-containing 5 OS=Mus musculus OX=1009... [more]
Q9BXW72.9e-2025.06Haloacid dehalogenase-like hydrolase domain-containing 5 OS=Homo sapiens OX=9606... [more]
Match NameE-valueIdentityDescription
A0A6J1JXI71.6e-17070.87uncharacterized protein YKR070W-like OS=Cucurbita maxima OX=3661 GN=LOC111489204... [more]
A0A6J1FF735.1e-16970.22uncharacterized protein YKR070W-like OS=Cucurbita moschata OX=3662 GN=LOC1114449... [more]
A0A5D3C5R25.3e-15070.15Hydrolase family protein / HAD-superfamily protein isoform 2 OS=Cucumis melo var... [more]
A0A1S3CK225.3e-15070.15uncharacterized protein YKR070W-like OS=Cucumis melo OX=3656 GN=LOC103501676 PE=... [more]
A0A0A0KU312.9e-14867.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G497050 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G45740.17.2e-11553.88hydrolase family protein / HAD-superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF13242Hydrolase_likecoord: 344..436
e-value: 4.7E-14
score: 52.2
NoneNo IPR availablePANTHERPTHR14269CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATEDcoord: 43..276
NoneNo IPR availablePANTHERPTHR14269:SF41HYDROLASE FAMILY PROTEIN / HAD-SUPERFAMILY PROTEINcoord: 43..276
NoneNo IPR availablePANTHERPTHR14269:SF41HYDROLASE FAMILY PROTEIN / HAD-SUPERFAMILY PROTEINcoord: 309..446
NoneNo IPR availablePANTHERPTHR14269CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATEDcoord: 309..446
IPR006353HAD-superfamily hydrolase, subfamily IIA, CECR5TIGRFAMTIGR01456TIGR01456coord: 61..264
e-value: 7.8E-58
score: 193.8
IPR006357HAD-superfamily hydrolase, subfamily IIAPFAMPF13344Hydrolase_6coord: 63..158
e-value: 2.8E-9
score: 37.0
IPR006357HAD-superfamily hydrolase, subfamily IIATIGRFAMTIGR01460TIGR01460coord: 63..258
e-value: 8.2E-33
score: 112.1
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 124..343
e-value: 5.7E-43
score: 148.4
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 313..437
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 63..239

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS025881.1MS025881.1mRNA