MS025645 (gene) Bitter gourd (TR) v1

BLAST of MS025645 vs. NCBI nr
Match: XP_038896981.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 357.8 bits (917), Expect = 8.7e-95
Identity = 178/273 (65.20%), Postives = 215/273 (78.75%), Query Frame = 0

Query: 6   LAGIFIGALLFLYALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEPLQKTL 65
           +AGIF   +LFLYALF  +     I + R KLPP  VGG WP+IGHLH+L+ +EP   T 
Sbjct: 13  IAGIF-SVVLFLYALF--IISTRVIGAHRNKLPPEPVGG-WPLIGHLHLLNAAEPAHITF 72

Query: 66  AKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYAMFA 125
           AK+AD YGPIF LKLGM + L++SSWEIA ECFTTND +FASRPKL+AS+LLGYDYAMF 
Sbjct: 73  AKLADTYGPIFTLKLGMKKALIVSSWEIANECFTTNDKIFASRPKLVASKLLGYDYAMFG 132

Query: 126 FSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWV---GGGGRKLLV 185
           FS Y  +WRHVRK+  ++LL+NHRLD LQHIR  EVH  IKK YEL +       +K LV
Sbjct: 133 FSPYGPHWRHVRKLATVELLTNHRLDQLQHIRAFEVHNSIKKLYELCIINKNNNEQKTLV 192

Query: 186 EMRTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHEQFRKALRNFFELFGVFVPSDSLPF 245
           EM+T F DI L+ IFR+VVGKRFSTA DG DN D+E++RKALR+FFE FGVFVPSDS PF
Sbjct: 193 EMKTWFGDIILNTIFRIVVGKRFSTAVDGTDN-DNEEYRKALRDFFEWFGVFVPSDSFPF 252

Query: 246 VKWLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR 276
           +KWLDLGGH+KAMKKTAK+LD++ DKWL+EH++
Sbjct: 253 LKWLDLGGHEKAMKKTAKILDNVFDKWLKEHQQ 280

BLAST of MS025645 vs. NCBI nr
Match: XP_038905619.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 356.3 bits (913), Expect = 2.5e-94
Identity = 176/269 (65.43%), Postives = 209/269 (77.70%), Query Frame = 0

Query: 8   GIFIGALLFLYALFRPLYGGLFISS-QRKKLPPPEVGGAWPVIGHLHMLSPSEPLQKTLA 67
           G+ I  LLFLYALF  L   L  +S QRKKL PPEVGGAWPVIGHLH+L  SEP  K L 
Sbjct: 10  GMLISFLLFLYALFWVLSRRLLSNSQQRKKLHPPEVGGAWPVIGHLHLLGGSEPTYKILE 69

Query: 68  KMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYAMFAF 127
           KMADAYGPIF LK+G HR +V+S+WEIA+ECFTTND VFASRPK++A++ +GYD  MFAF
Sbjct: 70  KMADAYGPIFTLKMGSHRAVVVSNWEIAKECFTTNDRVFASRPKVVAAKHMGYDNTMFAF 129

Query: 128 SSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRKLLVEMRT 187
           S Y   WRH+RKI   ++LSNHRLDLLQHIRRSEV   I K +ELWV  GG K+LVEM+ 
Sbjct: 130 SQYGPLWRHIRKITTHEILSNHRLDLLQHIRRSEVQSSINKLHELWVVKGGEKVLVEMKN 189

Query: 188 SFADITLDVIFRLVVGKRFSTAFDGG--DNTDHEQFRKALRNFFELFGVFVPSDSLPFVK 247
            F ++TL++IFR+VVGK FSTA++G   D  +  QF+ ALR+F ELF  FVPSDS PF+ 
Sbjct: 190 WFGELTLNIIFRMVVGKPFSTAYEGSGDDGAEGVQFQVALRDFLELFMAFVPSDSFPFLS 249

Query: 248 WLDLGGHKKAMKKTAKVLDDMMDKWLEEH 274
           WLDLGG++KAMKKTAKVLD  +DKWL EH
Sbjct: 250 WLDLGGYEKAMKKTAKVLDKTLDKWLREH 278

BLAST of MS025645 vs. NCBI nr
Match: XP_022982223.1 (cytochrome P450 CYP82D47-like [Cucurbita maxima])

HSP 1 Score: 355.1 bits (910), Expect = 5.6e-94
Identity = 180/271 (66.42%), Postives = 213/271 (78.60%), Query Frame = 0

Query: 5   SLAGIFIGALLFLYALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEPLQKT 64
           ++AGIF   L+FLYALF        +SS  K+L PPE  GAWPVIGHLH+LS +EP   T
Sbjct: 14  AVAGIFT-LLIFLYALFNI---STKLSSNPKRL-PPEPAGAWPVIGHLHLLSTTEPAHIT 73

Query: 65  LAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYAMF 124
           LAKMAD YGPIF LKLGMHRTL++SSWEIARECFTT D +FASRPKL+AS+LLGY+YAM 
Sbjct: 74  LAKMADVYGPIFTLKLGMHRTLIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMM 133

Query: 125 AFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRKLLVEM 184
             S Y  +WRHVRK+  L+L +NHRL  LQH+R SEV   IKK YEL  G G  K LV+M
Sbjct: 134 GLSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVESSIKKLYEL-CGNGKEKALVDM 193

Query: 185 RTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHEQFRKALRNFFELFGVFVPSDSLPFVK 244
           +T F DITL+ IFR+VVGKRFSTA +GG N   E++RKALR FFE FGVFVPSDS PF+K
Sbjct: 194 KTWFGDITLNTIFRVVVGKRFSTAVEGGGN---EEYRKALREFFEWFGVFVPSDSFPFLK 253

Query: 245 WLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR 276
           WLDLGGH+KAMKKTA+VLD+++DKW+EE R+
Sbjct: 254 WLDLGGHEKAMKKTAEVLDEVLDKWVEERRK 275

BLAST of MS025645 vs. NCBI nr
Match: XP_023523452.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 350.1 bits (897), Expect = 1.8e-92
Identity = 178/271 (65.68%), Postives = 212/271 (78.23%), Query Frame = 0

Query: 5   SLAGIFIGALLFLYALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEPLQKT 64
           ++AGIF   L+FLYALF  +Y  L  SS  K+L PPE  GAWPVIGHLH+LS +EP   T
Sbjct: 11  AVAGIF-ALLIFLYALFN-IYKRL--SSNPKRL-PPEPAGAWPVIGHLHLLSTTEPAHIT 70

Query: 65  LAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYAMF 124
           LAKMAD YGPIF L+LGMH+ L++SSWEIARECFTT D +FASRPKL+AS+LLGY+YAM 
Sbjct: 71  LAKMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMM 130

Query: 125 AFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRKLLVEM 184
             S Y  +WRHVRK+  L+L +NHRL  LQH+R SEV   IKK Y L  G G  K LV+M
Sbjct: 131 GLSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVESAIKKLYAL-CGNGKEKALVDM 190

Query: 185 RTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHEQFRKALRNFFELFGVFVPSDSLPFVK 244
           +T F DITL+ IFR+VVGKRFSTA +GG N   +++RKALR FFE FGVFVPSDS PF+K
Sbjct: 191 KTWFGDITLNTIFRVVVGKRFSTAVEGGGN---DEYRKALREFFEWFGVFVPSDSFPFLK 250

Query: 245 WLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR 276
           WLDLGGH+KAMKKTA+VLDD+ DKW+EE R+
Sbjct: 251 WLDLGGHEKAMKKTAEVLDDVFDKWVEERRK 272

BLAST of MS025645 vs. NCBI nr
Match: XP_008464765.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo] >KAA0032725.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa] >TYJ98756.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa])

HSP 1 Score: 349.0 bits (894), Expect = 4.0e-92
Identity = 171/281 (60.85%), Postives = 223/281 (79.36%), Query Frame = 0

Query: 3   PYSLAGIFIGALLFLYALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEPLQ 62
           P ++ GIF   ++FLYALF+   GG  + ++ +K  PPEVGGA P+IGHLH+L+ +EP  
Sbjct: 9   PDTVTGIF-ALIIFLYALFKIYRGG--VGARHRKRLPPEVGGALPLIGHLHLLAKNEPAH 68

Query: 63  KTLAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYA 122
           KT AKMADAYGPIF L+LG++  L++S+WEIARECFTTND +FASRPKL+AS+LLGYDYA
Sbjct: 69  KTFAKMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYA 128

Query: 123 MFAFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRKLLV 182
           MF  S Y  +WRHVRK+ +L+LLSN+RL+ LQHIR SEV   +K  YEL +    +  LV
Sbjct: 129 MFGLSPYGPHWRHVRKLAMLELLSNYRLEKLQHIRVSEVQTSMKNLYELCL-KNKKNALV 188

Query: 183 EMRTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHEQFRKALRNFFELFGVFVPSDSLPF 242
           EM+T F DITL+ I R+VVGK+FSTA D  +N ++E++RKALR+FFE FGVFVPSDS PF
Sbjct: 189 EMKTWFGDITLNTISRIVVGKKFSTAVDASNN-ENEEYRKALRDFFEWFGVFVPSDSFPF 248

Query: 243 VKWLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR--GEIPVK 282
           +KWLDLGGH+KAMKKTAKVLD++ DKW+++H+   GE+ ++
Sbjct: 249 LKWLDLGGHEKAMKKTAKVLDEVFDKWIQDHKNNLGEVKME 284

BLAST of MS025645 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 236.5 bits (602), Expect = 3.8e-61
Identity = 120/279 (43.01%), Postives = 179/279 (64.16%), Query Frame = 0

Query: 5   SLAGIFIGALLFLY-ALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEP-LQ 64
           SL  +F+  L+F++ ALF+        S + K +  P   GAWP+IGHLH+LS  E  L 
Sbjct: 4   SLFSLFVPILVFVFIALFKK-------SKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLY 63

Query: 65  KTLAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYA 124
           +TL KMAD YGP  +L+LG   T V+SS+E+A++CFT ND   ASRP   A++ +GYD A
Sbjct: 64  RTLGKMADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCA 123

Query: 125 MFAFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWV-GGGGRKLL 184
           +F F+ Y  +WR +RKI  L+LLSN RL +L+H+R SE+   ++  Y LWV  GG   ++
Sbjct: 124 VFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVM 183

Query: 185 VEMRTSFADITLDVIFRLVVGKRFSTAFDGG-----DNTDHEQFRKALRNFFELFGVFVP 244
           V++++   D++L+++ R+V GKR+   F GG     D  +  Q RK + NFF L G+F  
Sbjct: 184 VDLKSWLEDMSLNMMVRMVAGKRY---FGGGSLSPEDAEEARQCRKGVANFFHLVGIFTV 243

Query: 245 SDSLPFVKWLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR 276
           SD+ P + W D  GH+K MK+T + LD ++++W+E HR+
Sbjct: 244 SDAFPKLGWFDFQGHEKEMKQTGRELDVILERWIENHRQ 272

BLAST of MS025645 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.5e-60
Identity = 118/280 (42.14%), Postives = 179/280 (63.93%), Query Frame = 0

Query: 5   SLAGIFIGALLFLY-ALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEP-LQ 64
           SL  +F+  L+F++ ALF+        S + K +  P   GAWP+IGHLH+L   E  L 
Sbjct: 4   SLFSLFVPILVFVFIALFKK-------SKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLY 63

Query: 65  KTLAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYA 124
           +TL KMAD YGP  +L+LG +   V+SS+E+A++CFT ND   ASRP   A++ +GY++A
Sbjct: 64  RTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFA 123

Query: 125 MFAFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRK-LL 184
           +F F+ Y  +WR +RKI  ++LLSN RL +L+H+R SE+   +K  Y LW   GG K ++
Sbjct: 124 VFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVM 183

Query: 185 VEMRTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHE------QFRKALRNFFELFGVFV 244
           V++++   D+TL++I R+V GKR+   F GG +   E      Q +KA+  FF L G+F 
Sbjct: 184 VDLKSWLEDMTLNMIVRMVAGKRY---FGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFT 243

Query: 245 PSDSLPFVKWLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR 276
            SD+ P + + DL GH+K MK+T   LD ++++W+E HR+
Sbjct: 244 VSDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQ 273

BLAST of MS025645 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.9e-60
Identity = 116/272 (42.65%), Postives = 170/272 (62.50%), Query Frame = 0

Query: 6   LAGIFIGALLFLYALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEPLQKTL 65
           L G F+    F Y +++            K   PP+  GAWP+IGHLH+L+  +   K L
Sbjct: 11  LLGFFVLLPCFFYFVWK--------KPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKIL 70

Query: 66  AKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYAMFA 125
           +  AD  GP+F ++LG+H+ LV+++ EIA+ECFTTND  F +RP  +A++++GY+Y M  
Sbjct: 71  SSFADKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLG 130

Query: 126 FSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRKLLVEMR 185
            + Y  YWR +RKI++L+ LSN RL  L+H+  SE+    K+ Y+LW        LV+M+
Sbjct: 131 VAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDFCLVDMK 190

Query: 186 TSFADITLDVIFRLVVGKRF--STAFDGGDNTDHEQFRKALRNFFELFGVFVPSDSLPFV 245
              AD+TL++  ++VVGKRF  S +    + T+     K LRN F L G FV SD LP++
Sbjct: 191 QWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYL 250

Query: 246 KWLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR 276
           +WLDLGGH+K MK+T K LD +   WL+EH+R
Sbjct: 251 RWLDLGGHEKEMKRTVKELDILFKGWLDEHKR 274

BLAST of MS025645 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 231.1 bits (588), Expect = 1.6e-59
Identity = 117/286 (40.91%), Postives = 169/286 (59.09%), Query Frame = 0

Query: 10  FIGALLFLYALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEPLQKTLAKMA 69
           FI  +  L+ LFR        S       PP + G+WP++GHL ++  ++   KTL  + 
Sbjct: 13  FISLISLLFFLFR-------FSKVSHTKEPPIISGSWPLLGHLPLMRNTQTPHKTLGALV 72

Query: 70  DAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYAMFAFSSY 129
           D YGPIF +KLG    LV+S+WE+A+ECFT ND+V +SRPK +A EL+ Y+ A   ++ Y
Sbjct: 73  DKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPY 132

Query: 130 DKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRK---------- 189
             YWR +RKIV L++LSN R++LL HIR SEV   IK+   +W      +          
Sbjct: 133 GAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKSS 192

Query: 190 -----------LLVEMRTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHEQFRKALRNFF 249
                      + VE++  FA +TL+++ R+VVGKR     D  +  + ++F + +R+F 
Sbjct: 193 STNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENIRDFM 252

Query: 250 ELFGVFVPSDSLPFVKWLDLGGHKKAMKKTAKVLDDMMDKWLEEHR 275
            L G F   D +PF+KWLDLGGH+K MKK AK  D M+++WLEEHR
Sbjct: 253 RLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHR 291

BLAST of MS025645 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 8.0e-59
Identity = 105/242 (43.39%), Postives = 157/242 (64.88%), Query Frame = 0

Query: 40  PEVGGAWPVIGHLHMLSPSEPLQKTLAKMADAYGPIFALKLGMHRTLVISSWEIARECFT 99
           P V GAWP++GHL +L+ S+   K L  +AD YGP+F +KLGM   LV+S+WE+++E FT
Sbjct: 39  PVVSGAWPILGHLSLLNGSQTPHKVLGALADKYGPLFTIKLGMKPALVLSNWEMSKELFT 98

Query: 100 TNDVVFASRPKLLASELLGYDYAMFAFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRS 159
           TND+  +SRPKL+A E++ Y+ A    + Y  YWR +RKIV  + LSN R++   HIR S
Sbjct: 99  TNDLAVSSRPKLVAVEVMSYNQAFVGLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVS 158

Query: 160 EVHGWIKKQYELWVGGG---GRKLLVEMRTSFADITLDVIFRLVVGKRFSTAFDGGDNTD 219
           EV   IK+ +++W  G     R  LV+++   A +T +++ R+VVGKR+           
Sbjct: 159 EVRTSIKELFDIWSNGNKNESRYTLVDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDK 218

Query: 220 HEQFRKALRNFFELFGVFVPSDSLPFVKWLDLGGHKKAMKKTAKVLDDMMDKWLEEHRRG 279
            ++F K +R F  L G F  +D +P ++WLDLGGH+KAMK  AK +D ++ +WLEEHR+ 
Sbjct: 219 AQRFMKNIREFMNLMGTFTVADGVPCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQK 278

BLAST of MS025645 vs. ExPASy TrEMBL
Match: A0A6J1J4B0 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111481116 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 2.7e-94
Identity = 180/271 (66.42%), Postives = 213/271 (78.60%), Query Frame = 0

Query: 5   SLAGIFIGALLFLYALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEPLQKT 64
           ++AGIF   L+FLYALF        +SS  K+L PPE  GAWPVIGHLH+LS +EP   T
Sbjct: 14  AVAGIFT-LLIFLYALFNI---STKLSSNPKRL-PPEPAGAWPVIGHLHLLSTTEPAHIT 73

Query: 65  LAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYAMF 124
           LAKMAD YGPIF LKLGMHRTL++SSWEIARECFTT D +FASRPKL+AS+LLGY+YAM 
Sbjct: 74  LAKMADVYGPIFTLKLGMHRTLIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMM 133

Query: 125 AFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRKLLVEM 184
             S Y  +WRHVRK+  L+L +NHRL  LQH+R SEV   IKK YEL  G G  K LV+M
Sbjct: 134 GLSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVESSIKKLYEL-CGNGKEKALVDM 193

Query: 185 RTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHEQFRKALRNFFELFGVFVPSDSLPFVK 244
           +T F DITL+ IFR+VVGKRFSTA +GG N   E++RKALR FFE FGVFVPSDS PF+K
Sbjct: 194 KTWFGDITLNTIFRVVVGKRFSTAVEGGGN---EEYRKALREFFEWFGVFVPSDSFPFLK 253

Query: 245 WLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR 276
           WLDLGGH+KAMKKTA+VLD+++DKW+EE R+
Sbjct: 254 WLDLGGHEKAMKKTAEVLDEVLDKWVEERRK 275

BLAST of MS025645 vs. ExPASy TrEMBL
Match: A0A5A7SU10 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00630 PE=3 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.9e-92
Identity = 171/281 (60.85%), Postives = 223/281 (79.36%), Query Frame = 0

Query: 3   PYSLAGIFIGALLFLYALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEPLQ 62
           P ++ GIF   ++FLYALF+   GG  + ++ +K  PPEVGGA P+IGHLH+L+ +EP  
Sbjct: 9   PDTVTGIF-ALIIFLYALFKIYRGG--VGARHRKRLPPEVGGALPLIGHLHLLAKNEPAH 68

Query: 63  KTLAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYA 122
           KT AKMADAYGPIF L+LG++  L++S+WEIARECFTTND +FASRPKL+AS+LLGYDYA
Sbjct: 69  KTFAKMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYA 128

Query: 123 MFAFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRKLLV 182
           MF  S Y  +WRHVRK+ +L+LLSN+RL+ LQHIR SEV   +K  YEL +    +  LV
Sbjct: 129 MFGLSPYGPHWRHVRKLAMLELLSNYRLEKLQHIRVSEVQTSMKNLYELCL-KNKKNALV 188

Query: 183 EMRTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHEQFRKALRNFFELFGVFVPSDSLPF 242
           EM+T F DITL+ I R+VVGK+FSTA D  +N ++E++RKALR+FFE FGVFVPSDS PF
Sbjct: 189 EMKTWFGDITLNTISRIVVGKKFSTAVDASNN-ENEEYRKALRDFFEWFGVFVPSDSFPF 248

Query: 243 VKWLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR--GEIPVK 282
           +KWLDLGGH+KAMKKTAKVLD++ DKW+++H+   GE+ ++
Sbjct: 249 LKWLDLGGHEKAMKKTAKVLDEVFDKWIQDHKNNLGEVKME 284

BLAST of MS025645 vs. ExPASy TrEMBL
Match: A0A1S3CM78 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502573 PE=3 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.9e-92
Identity = 171/281 (60.85%), Postives = 223/281 (79.36%), Query Frame = 0

Query: 3   PYSLAGIFIGALLFLYALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEPLQ 62
           P ++ GIF   ++FLYALF+   GG  + ++ +K  PPEVGGA P+IGHLH+L+ +EP  
Sbjct: 9   PDTVTGIF-ALIIFLYALFKIYRGG--VGARHRKRLPPEVGGALPLIGHLHLLAKNEPAH 68

Query: 63  KTLAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYA 122
           KT AKMADAYGPIF L+LG++  L++S+WEIARECFTTND +FASRPKL+AS+LLGYDYA
Sbjct: 69  KTFAKMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYA 128

Query: 123 MFAFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRKLLV 182
           MF  S Y  +WRHVRK+ +L+LLSN+RL+ LQHIR SEV   +K  YEL +    +  LV
Sbjct: 129 MFGLSPYGPHWRHVRKLAMLELLSNYRLEKLQHIRVSEVQTSMKNLYELCL-KNKKNALV 188

Query: 183 EMRTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHEQFRKALRNFFELFGVFVPSDSLPF 242
           EM+T F DITL+ I R+VVGK+FSTA D  +N ++E++RKALR+FFE FGVFVPSDS PF
Sbjct: 189 EMKTWFGDITLNTISRIVVGKKFSTAVDASNN-ENEEYRKALRDFFEWFGVFVPSDSFPF 248

Query: 243 VKWLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR--GEIPVK 282
           +KWLDLGGH+KAMKKTAKVLD++ DKW+++H+   GE+ ++
Sbjct: 249 LKWLDLGGHEKAMKKTAKVLDEVFDKWIQDHKNNLGEVKME 284

BLAST of MS025645 vs. ExPASy TrEMBL
Match: A0A6J1FKD2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111446070 PE=3 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 1.3e-91
Identity = 175/271 (64.58%), Postives = 209/271 (77.12%), Query Frame = 0

Query: 5   SLAGIFIGALLFLYALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEPLQKT 64
           ++AGIF   L+FLY LF        +SS  K+L PPE  GAWPVIGHLH+LS +EP   T
Sbjct: 11  AVAGIF-ALLIFLYVLFNI---SKRLSSNPKRL-PPEPAGAWPVIGHLHLLSTTEPAHIT 70

Query: 65  LAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYAMF 124
           LAKMAD YGPIF L+LGMH+ L++SSWEIARECFTT D +FASRPKL+AS+LLGY+YAM 
Sbjct: 71  LAKMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMM 130

Query: 125 AFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRKLLVEM 184
             S Y  +WRHVRK+  L+L +NHRL  LQH+R SEV   IKK YEL  G G  K LV+M
Sbjct: 131 GLSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVDSSIKKLYEL-CGNGKEKALVDM 190

Query: 185 RTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHEQFRKALRNFFELFGVFVPSDSLPFVK 244
            T F DITL+ IFR+VVGKRFSTA +GG N   +++RKALR FFE FGVFVPSDS PF+K
Sbjct: 191 TTWFGDITLNTIFRVVVGKRFSTAVEGGGN---DEYRKALREFFEWFGVFVPSDSFPFLK 250

Query: 245 WLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR 276
           WLDLGGH+KAMKKTA+VLD++ DKW+EE R+
Sbjct: 251 WLDLGGHEKAMKKTAEVLDEVFDKWVEERRK 272

BLAST of MS025645 vs. ExPASy TrEMBL
Match: A0A0A0LG04 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852630 PE=3 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 5.9e-89
Identity = 168/276 (60.87%), Postives = 214/276 (77.54%), Query Frame = 0

Query: 3   PYSLAGIFIGALLFLYALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEPLQ 62
           P  +A IF   ++FLYALF+ +Y G   +  RK+L PPEVGGA P+IGHLH+L  +EP  
Sbjct: 15  PDVVARIF-ALIIFLYALFK-IYRGRARAHHRKRL-PPEVGGALPLIGHLHLLDKNEPAH 74

Query: 63  KTLAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYA 122
            T AKMADAYGPIF L+LG++  L++S+WEIARECFTTND +FASRPKL+AS+LLGYDYA
Sbjct: 75  ITFAKMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYA 134

Query: 123 MFAFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWV---GGGGRK 182
           M   S Y  +WRHVRK+ +L+LL++HRL  LQHIR SEV   IK  YEL +       + 
Sbjct: 135 MLGLSPYGPHWRHVRKLTMLELLTSHRLQKLQHIRVSEVQTSIKNLYELCLKNKKNNEKN 194

Query: 183 LLVEMRTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHEQFRKALRNFFELFGVFVPSDS 242
            LVEM+T F DITL+ I R+VVGK+FSTA D  +  ++E++RKALR+FFE FGVFVPSDS
Sbjct: 195 ALVEMKTWFGDITLNTISRIVVGKQFSTAVDVSNTNENEEYRKALRDFFEWFGVFVPSDS 254

Query: 243 LPFVKWLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR 276
            PF+KWLDLGGH+KAMKKTA+VLD++ DKW++EH++
Sbjct: 255 FPFLKWLDLGGHEKAMKKTAQVLDEVFDKWIQEHQQ 287

BLAST of MS025645 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 236.5 bits (602), Expect = 2.7e-62
Identity = 120/279 (43.01%), Postives = 179/279 (64.16%), Query Frame = 0

Query: 5   SLAGIFIGALLFLY-ALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEP-LQ 64
           SL  +F+  L+F++ ALF+        S + K +  P   GAWP+IGHLH+LS  E  L 
Sbjct: 4   SLFSLFVPILVFVFIALFKK-------SKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLY 63

Query: 65  KTLAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYA 124
           +TL KMAD YGP  +L+LG   T V+SS+E+A++CFT ND   ASRP   A++ +GYD A
Sbjct: 64  RTLGKMADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCA 123

Query: 125 MFAFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWV-GGGGRKLL 184
           +F F+ Y  +WR +RKI  L+LLSN RL +L+H+R SE+   ++  Y LWV  GG   ++
Sbjct: 124 VFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVM 183

Query: 185 VEMRTSFADITLDVIFRLVVGKRFSTAFDGG-----DNTDHEQFRKALRNFFELFGVFVP 244
           V++++   D++L+++ R+V GKR+   F GG     D  +  Q RK + NFF L G+F  
Sbjct: 184 VDLKSWLEDMSLNMMVRMVAGKRY---FGGGSLSPEDAEEARQCRKGVANFFHLVGIFTV 243

Query: 245 SDSLPFVKWLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR 276
           SD+ P + W D  GH+K MK+T + LD ++++W+E HR+
Sbjct: 244 SDAFPKLGWFDFQGHEKEMKQTGRELDVILERWIENHRQ 272

BLAST of MS025645 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 234.6 bits (597), Expect = 1.0e-61
Identity = 118/280 (42.14%), Postives = 179/280 (63.93%), Query Frame = 0

Query: 5   SLAGIFIGALLFLY-ALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEP-LQ 64
           SL  +F+  L+F++ ALF+        S + K +  P   GAWP+IGHLH+L   E  L 
Sbjct: 4   SLFSLFVPILVFVFIALFKK-------SKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLY 63

Query: 65  KTLAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYA 124
           +TL KMAD YGP  +L+LG +   V+SS+E+A++CFT ND   ASRP   A++ +GY++A
Sbjct: 64  RTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFA 123

Query: 125 MFAFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRK-LL 184
           +F F+ Y  +WR +RKI  ++LLSN RL +L+H+R SE+   +K  Y LW   GG K ++
Sbjct: 124 VFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVM 183

Query: 185 VEMRTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHE------QFRKALRNFFELFGVFV 244
           V++++   D+TL++I R+V GKR+   F GG +   E      Q +KA+  FF L G+F 
Sbjct: 184 VDLKSWLEDMTLNMIVRMVAGKRY---FGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFT 243

Query: 245 PSDSLPFVKWLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR 276
            SD+ P + + DL GH+K MK+T   LD ++++W+E HR+
Sbjct: 244 VSDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQ 273

BLAST of MS025645 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 200.3 bits (508), Expect = 2.2e-51
Identity = 111/280 (39.64%), Postives = 170/280 (60.71%), Query Frame = 0

Query: 5   SLAGIFIGALLFLY-ALFRPLYGGLFISSQRKKLPPPEVGGAWPVIGHLHMLSPSEP-LQ 64
           SL  +F+  L+F++ ALF+        S + K +  P   GAWP+IGHLH+L   E  L 
Sbjct: 4   SLFSLFVSILVFVFIALFKK-------SKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLY 63

Query: 65  KTLAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYA 124
           +TL KMAD YGP  +L+LG   T V SS+E+A++CFT ND   AS     A++ +GY   
Sbjct: 64  RTLGKMADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGY--- 123

Query: 125 MFAFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWV-GGGGRKLL 184
                    +W  +RKI +++LLSN RL +L ++R SE+   +K  Y LWV  GG   ++
Sbjct: 124 --------VFWLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVM 183

Query: 185 VEMRTSFADITLDVIFRLVVGKRFSTAFDGG--DNTDH----EQFRKALRNFFELFGVFV 244
           V++++   D+  ++I R+V GKR+   F GG  ++++H     Q+RK +  FF L G+F 
Sbjct: 184 VDLKSWLEDMIANMIMRMVAGKRY---FGGGGAESSEHTEEARQWRKGIAKFFHLVGIFT 243

Query: 245 PSDSLPFVKWLDLGGHKKAMKKTAKVLDDMMDKWLEEHRR 276
            SD+ P + WLDL GH+K MK+T + LD ++++W+E HR+
Sbjct: 244 VSDAFPKLGWLDLQGHEKEMKQTRRELDVILERWIENHRQ 261

BLAST of MS025645 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 174.1 bits (440), Expect = 1.7e-43
Identity = 99/270 (36.67%), Postives = 154/270 (57.04%), Query Frame = 0

Query: 9   IFIGALLFLYALFRPLYGGLFISSQRKKLPP-----PEVGGAWPVIGHLHMLSPSEPLQK 68
           +F    L L++L   ++G +F+  Q  +        PE  GA P+ GHLH+L   + L K
Sbjct: 4   LFSTLQLSLFSLALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCK 63

Query: 69  TLAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYAM 128
            LA M+  +GPIF+LKLG +R +V S  +  ++CFTTND+  A+RP +     +GY+ A 
Sbjct: 64  KLAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNAS 123

Query: 129 FAFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRKLLVE 188
              + Y  YWR +RKIV + L SNH +++L HIR SEV+  IK  Y+    G G   +V+
Sbjct: 124 LTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYK----GNGGTSIVK 183

Query: 189 MRTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHEQFRKALRNFFELFGVFVPSDSLPFV 248
           +   F  +T ++I R +VGKR      G  N+D  ++++AL++   L  + +  D +P++
Sbjct: 184 IDMLFEFLTFNIILRKMVGKRIGF---GEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWL 243

Query: 249 KWLDLGGHKKAMKKTAKVLDDMMDKWLEEH 274
            WLD   + + MK+  K LD +  KWL EH
Sbjct: 244 GWLDFAKNSQ-MKRLFKELDSVNTKWLHEH 265

BLAST of MS025645 vs. TAIR 10
Match: AT3G25180.2 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 174.1 bits (440), Expect = 1.7e-43
Identity = 99/270 (36.67%), Postives = 154/270 (57.04%), Query Frame = 0

Query: 9   IFIGALLFLYALFRPLYGGLFISSQRKKLPP-----PEVGGAWPVIGHLHMLSPSEPLQK 68
           +F    L L++L   ++G +F+  Q  +        PE  GA P+ GHLH+L   + L K
Sbjct: 4   LFSTLQLSLFSLALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCK 63

Query: 69  TLAKMADAYGPIFALKLGMHRTLVISSWEIARECFTTNDVVFASRPKLLASELLGYDYAM 128
            LA M+  +GPIF+LKLG +R +V S  +  ++CFTTND+  A+RP +     +GY+ A 
Sbjct: 64  KLAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNAS 123

Query: 129 FAFSSYDKYWRHVRKIVVLQLLSNHRLDLLQHIRRSEVHGWIKKQYELWVGGGGRKLLVE 188
              + Y  YWR +RKIV + L SNH +++L HIR SEV+  IK  Y+    G G   +V+
Sbjct: 124 LTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYK----GNGGTSIVK 183

Query: 189 MRTSFADITLDVIFRLVVGKRFSTAFDGGDNTDHEQFRKALRNFFELFGVFVPSDSLPFV 248
           +   F  +T ++I R +VGKR      G  N+D  ++++AL++   L  + +  D +P++
Sbjct: 184 IDMLFEFLTFNIILRKMVGKRIGF---GEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWL 243

Query: 249 KWLDLGGHKKAMKKTAKVLDDMMDKWLEEH 274
            WLD   + + MK+  K LD +  KWL EH
Sbjct: 244 GWLDFAKNSQ-MKRLFKELDSVNTKWLHEH 265