MS025592 (gene) Bitter gourd (TR) v1

Overview
NameMS025592
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionU-box domain-containing protein 52-like
Locationscaffold4: 1301391 .. 1303983 (+)
RNA-Seq ExpressionMS025592
SyntenyMS025592
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGTTCGGCGTCTCGGCCTGCTCCGACGATAAGTCCTCTCAGAGGTCAATTAGCGCAACAATGCAGCGCCAAATCTGATGCAACCGAATTCCATTCTGCAACTGGAACTGCTGCAAGAGGTAATTTCTATTTCTATTTCTATTTCTATTTCAAACCAAATTTCTCTGTTTCTGTGATCTTCAATTTTCTCTCCAGTTTCCATCGAAAAGCCACCCGTTGGCCTTGATTTTCCCTCTAAACCACCGCAGGACGAGCTCGATTTCATGAAGTCAGTATTCCCTCTTTAACTTTACGTAATTCTTCAGATATGGATTATGATGTTGTACAAGTTTCAGTTTGTTGTTGCGAAACTGCTAGTTTACCCAAAAGAGGAACAAAAATATTGTTGAAGAAGCAGAAAATAGTGGTGTTGTTCTCGTTAGTTTGGTAGGCTAGGGTAGATTGATGAGCAAAAAATGTTGTTGGAAGCATTGTTCTTCTTGTACATGTTTCTGTTTGTTAGTTTGGTAAACTTGGCTAGATTGATGAGCAAAATGTTGTTGGAATGGAAGCAGGTCGCCATTCACCAGGAAAGGATACAATTACAAGGCATCGTATGGAGACTTGTCGATGCATGATTCGGATATATCATTTGTTAGCTCGGGAAGGCCGAGTATTGATCGCATATTTCCTTCACTTTATGACAATCAAGATGTAGGGAGAGTTACCCCACGGATGTCGAGCAGTACAGATCTTGACTTGACTCACAGCTTCGAGTCGCTGCATATGGGACGGATGTCGCTCGATATGAGCTGTCCCTCCGAGTTCTCTTCCATCTCGCAAGATAGTGACAGACTCTCAATCTCATCACAGGCTATGGTAAGTTTTTTAGTCACTTTTGTAATTGTTTTGTAGTGAACTCTCCATTCAGTATTGTATCTCTTTTTCTAATGATGCAGGAAGATGTTGAGGCCGAGATGCGGCGGCTGAAGCTTGAACTGAAGCAGACCATGGAAATGTACAGTACAGCCTGCAAGGAAGCGCTTACAGCAAAACAAAAGGTAACTGGAATCGATTGATTAACGTGGTATGTTGATTGTCTTCGATGGCTGCAGCACAATATATCATATGGTTTCCTTTCCAATGCAGGCAGTTGAGCTACAGCGCTGGAAATTAGAAGAAGAGCGGAGATTAGAAGAGGCGAGATTAGCCGGGGAAGCTGCATTGGCACTAGCAGAACAGGAGAAGGCGAAGTCGAAAGCAGCCATCGAAGCTGCTGAAGCAGCTAGGAGGATTGCGGAATTGGAAGCACAGAAGAGAATGAAGGCTGAAATGAGAGCACTAAAAGAAGCTGAAGAGAAGAAAAAGGCAGTTGATGCATTGTCACAAAATGATATCAGGTATAGAAAGTATACGATCGAGGAGATCGAAGCGGCAACGGAGTTCTTTTCCGAATCCCGCAAGATCGGAGAAGGAGGTTATGGGCCTGTATATAAGTGTGATCTCGATCATACTCCTGTTGCGATTAAGGTTCTACGTCCTGATGCAACTCAAGGACGGTCACAGTTTAAACAAGAGGTAAGCGATCGAGCAGTTTAAAGAAGAGAAGAGAAGCCTTCAATTAATGAATTAGTTTGTTCTAACTTAAAGAAAACCATTGAACAATCACAGGTAGAAGTTCTTAGCTGCATTCGGCATCCGAACATGGTTCTCCTCCTTGGAGCTTGCCCTGAGTATGGCTGCTTGGTGTATGAGTTCATGGCGAATGGTAGCTTAGAGGACCGACTTGCACGACGAGGAAACACGCCTCCTCTTTCATGGCAGCTGAGGTTTAAAATTGCAGCTGAAATTGGAACGGCATTGTTGTTCCTACACCAGACAAAGCCAGAGCCTCTTGTGCACCGCGATCTCAAACCCGCCAACATCTTGCTTGACCGTAACTTCGTGAGCAAGATCAGTGATGTTGGCTTGGCCAGGCTTGTACCTCCATCAGTTGCCGATAACGTAACGCAATATCGAATGACATCAACTGCTGGAACATTTTGCTACATCGACCCCGAGTATCAACAGACGGGTATGCTCGGTGTGAAATCTGACATATACTCCCTAGGTATCATGTTTCTCCAACTGATAACTTCCAGGCCTCCAATGGGTTTGACTCACCATGTCGAAAGAGCTATTGAGAAGGGGACGTTCGCGAGTATTCTCGATCCAACCGTCTCCGATTGGCCTGTCGAAGAAGCTCTGTCTTTCGCCAAGATGTCACTCAAGTGTGCTGAGTTAAGACGTAAAGATAGGCCCGATCTCGGGAAGGCCATCCTGCCAGAGCTTAACAGATTGAGAACCATTGCAGAAGACACAATGCATCCAACACTGATGGGAGGAGACACAAGTCCCTCCCGTAGCCACAGCCAAGTTTCTCTACAGTTGGTAAGTTCAAAAATGACCAAAATTTTAGGTTCTAATCAGTCAAAAGAAACTATTTCTACTCTTTAATCCTGAGCACGTATTGGATAATAACTATTTTTAACACAACGATCGTTTTATTGCAGGGGTCAATGACTTCGTCCCATCAATCATTCTATAGCTCGAAAAATCATTCTGTTACTTGA

mRNA sequence

ATGCGTTCGGCGTCTCGGCCTGCTCCGACGATAAGTCCTCTCAGAGGTCAATTAGCGCAACAATGCAGCGCCAAATCTGATGCAACCGAATTCCATTCTGCAACTGGAACTGCTGCAAGAGTTTCCATCGAAAAGCCACCCGTTGGCCTTGATTTTCCCTCTAAACCACCGCAGGACGAGCTCGATTTCATGAAGTCGCCATTCACCAGGAAAGGATACAATTACAAGGCATCGTATGGAGACTTGTCGATGCATGATTCGGATATATCATTTGTTAGCTCGGGAAGGCCGAGTATTGATCGCATATTTCCTTCACTTTATGACAATCAAGATGTAGGGAGAGTTACCCCACGGATGTCGAGCAGTACAGATCTTGACTTGACTCACAGCTTCGAGTCGCTGCATATGGGACGGATGTCGCTCGATATGAGCTGTCCCTCCGAGTTCTCTTCCATCTCGCAAGATAGTGACAGACTCTCAATCTCATCACAGGCTATGGAAGATGTTGAGGCCGAGATGCGGCGGCTGAAGCTTGAACTGAAGCAGACCATGGAAATGTACAGTACAGCCTGCAAGGAAGCGCTTACAGCAAAACAAAAGGCAGTTGAGCTACAGCGCTGGAAATTAGAAGAAGAGCGGAGATTAGAAGAGGCGAGATTAGCCGGGGAAGCTGCATTGGCACTAGCAGAACAGGAGAAGGCGAAGTCGAAAGCAGCCATCGAAGCTGCTGAAGCAGCTAGGAGGATTGCGGAATTGGAAGCACAGAAGAGAATGAAGGCTGAAATGAGAGCACTAAAAGAAGCTGAAGAGAAGAAAAAGGCAGTTGATGCATTGTCACAAAATGATATCAGGTATAGAAAGTATACGATCGAGGAGATCGAAGCGGCAACGGAGTTCTTTTCCGAATCCCGCAAGATCGGAGAAGGAGGTTATGGGCCTGTATATAAGTGTGATCTCGATCATACTCCTGTTGCGATTAAGGTTCTACGTCCTGATGCAACTCAAGGACGGTCACAGTTTAAACAAGAGGTAGAAGTTCTTAGCTGCATTCGGCATCCGAACATGGTTCTCCTCCTTGGAGCTTGCCCTGAGTATGGCTGCTTGGTGTATGAGTTCATGGCGAATGGTAGCTTAGAGGACCGACTTGCACGACGAGGAAACACGCCTCCTCTTTCATGGCAGCTGAGGTTTAAAATTGCAGCTGAAATTGGAACGGCATTGTTGTTCCTACACCAGACAAAGCCAGAGCCTCTTGTGCACCGCGATCTCAAACCCGCCAACATCTTGCTTGACCGTAACTTCGTGAGCAAGATCAGTGATGTTGGCTTGGCCAGGCTTGTACCTCCATCAGTTGCCGATAACGTAACGCAATATCGAATGACATCAACTGCTGGAACATTTTGCTACATCGACCCCGAGTATCAACAGACGGGTATGCTCGGTGTGAAATCTGACATATACTCCCTAGGTATCATGTTTCTCCAACTGATAACTTCCAGGCCTCCAATGGGTTTGACTCACCATGTCGAAAGAGCTATTGAGAAGGGGACGTTCGCGAGTATTCTCGATCCAACCGTCTCCGATTGGCCTGTCGAAGAAGCTCTGTCTTTCGCCAAGATGTCACTCAAGTGTGCTGAGTTAAGACGTAAAGATAGGCCCGATCTCGGGAAGGCCATCCTGCCAGAGCTTAACAGATTGAGAACCATTGCAGAAGACACAATGCATCCAACACTGATGGGAGGAGACACAAGTCCCTCCCGTAGCCACAGCCAAGTTTCTCTACAGTTGGGGTCAATGACTTCGTCCCATCAATCATTCTATAGCTCGAAAAATCATTCTGTTACTTGA

Coding sequence (CDS)

ATGCGTTCGGCGTCTCGGCCTGCTCCGACGATAAGTCCTCTCAGAGGTCAATTAGCGCAACAATGCAGCGCCAAATCTGATGCAACCGAATTCCATTCTGCAACTGGAACTGCTGCAAGAGTTTCCATCGAAAAGCCACCCGTTGGCCTTGATTTTCCCTCTAAACCACCGCAGGACGAGCTCGATTTCATGAAGTCGCCATTCACCAGGAAAGGATACAATTACAAGGCATCGTATGGAGACTTGTCGATGCATGATTCGGATATATCATTTGTTAGCTCGGGAAGGCCGAGTATTGATCGCATATTTCCTTCACTTTATGACAATCAAGATGTAGGGAGAGTTACCCCACGGATGTCGAGCAGTACAGATCTTGACTTGACTCACAGCTTCGAGTCGCTGCATATGGGACGGATGTCGCTCGATATGAGCTGTCCCTCCGAGTTCTCTTCCATCTCGCAAGATAGTGACAGACTCTCAATCTCATCACAGGCTATGGAAGATGTTGAGGCCGAGATGCGGCGGCTGAAGCTTGAACTGAAGCAGACCATGGAAATGTACAGTACAGCCTGCAAGGAAGCGCTTACAGCAAAACAAAAGGCAGTTGAGCTACAGCGCTGGAAATTAGAAGAAGAGCGGAGATTAGAAGAGGCGAGATTAGCCGGGGAAGCTGCATTGGCACTAGCAGAACAGGAGAAGGCGAAGTCGAAAGCAGCCATCGAAGCTGCTGAAGCAGCTAGGAGGATTGCGGAATTGGAAGCACAGAAGAGAATGAAGGCTGAAATGAGAGCACTAAAAGAAGCTGAAGAGAAGAAAAAGGCAGTTGATGCATTGTCACAAAATGATATCAGGTATAGAAAGTATACGATCGAGGAGATCGAAGCGGCAACGGAGTTCTTTTCCGAATCCCGCAAGATCGGAGAAGGAGGTTATGGGCCTGTATATAAGTGTGATCTCGATCATACTCCTGTTGCGATTAAGGTTCTACGTCCTGATGCAACTCAAGGACGGTCACAGTTTAAACAAGAGGTAGAAGTTCTTAGCTGCATTCGGCATCCGAACATGGTTCTCCTCCTTGGAGCTTGCCCTGAGTATGGCTGCTTGGTGTATGAGTTCATGGCGAATGGTAGCTTAGAGGACCGACTTGCACGACGAGGAAACACGCCTCCTCTTTCATGGCAGCTGAGGTTTAAAATTGCAGCTGAAATTGGAACGGCATTGTTGTTCCTACACCAGACAAAGCCAGAGCCTCTTGTGCACCGCGATCTCAAACCCGCCAACATCTTGCTTGACCGTAACTTCGTGAGCAAGATCAGTGATGTTGGCTTGGCCAGGCTTGTACCTCCATCAGTTGCCGATAACGTAACGCAATATCGAATGACATCAACTGCTGGAACATTTTGCTACATCGACCCCGAGTATCAACAGACGGGTATGCTCGGTGTGAAATCTGACATATACTCCCTAGGTATCATGTTTCTCCAACTGATAACTTCCAGGCCTCCAATGGGTTTGACTCACCATGTCGAAAGAGCTATTGAGAAGGGGACGTTCGCGAGTATTCTCGATCCAACCGTCTCCGATTGGCCTGTCGAAGAAGCTCTGTCTTTCGCCAAGATGTCACTCAAGTGTGCTGAGTTAAGACGTAAAGATAGGCCCGATCTCGGGAAGGCCATCCTGCCAGAGCTTAACAGATTGAGAACCATTGCAGAAGACACAATGCATCCAACACTGATGGGAGGAGACACAAGTCCCTCCCGTAGCCACAGCCAAGTTTCTCTACAGTTGGGGTCAATGACTTCGTCCCATCAATCATTCTATAGCTCGAAAAATCATTCTGTTACTTGA

Protein sequence

MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGLDFPSKPPQDELDFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDNQDVGRVTPRMSSSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRYRKYTIEEIEAATEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRSHSQVSLQLGSMTSSHQSFYSSKNHSVT
Homology
BLAST of MS025592 vs. NCBI nr
Match: XP_022144401.1 (U-box domain-containing protein 52-like [Momordica charantia])

HSP 1 Score: 1184.9 bits (3064), Expect = 0.0e+00
Identity = 615/615 (100.00%), Postives = 615/615 (100.00%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGLDFPSKPPQDE 60
           MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGLDFPSKPPQDE
Sbjct: 162 MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGLDFPSKPPQDE 221

Query: 61  LDFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDNQDVGRVTPRMS 120
           LDFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDNQDVGRVTPRMS
Sbjct: 222 LDFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDNQDVGRVTPRMS 281

Query: 121 SSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAMEDVEAEMRRLKLEL 180
           SSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAMEDVEAEMRRLKLEL
Sbjct: 282 SSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAMEDVEAEMRRLKLEL 341

Query: 181 KQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSKAAI 240
           KQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSKAAI
Sbjct: 342 KQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSKAAI 401

Query: 241 EAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRYRKYTIEEIEAATEFF 300
           EAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRYRKYTIEEIEAATEFF
Sbjct: 402 EAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRYRKYTIEEIEAATEFF 461

Query: 301 SESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLG 360
           SESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLG
Sbjct: 462 SESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLG 521

Query: 361 ACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVH 420
           ACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVH
Sbjct: 522 ACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVH 581

Query: 421 RDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGML 480
           RDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGML
Sbjct: 582 RDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGML 641

Query: 481 GVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALSFAKM 540
           GVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALSFAKM
Sbjct: 642 GVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALSFAKM 701

Query: 541 SLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRSHSQVSLQLGSMT 600
           SLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRSHSQVSLQLGSMT
Sbjct: 702 SLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRSHSQVSLQLGSMT 761

Query: 601 SSHQSFYSSKNHSVT 616
           SSHQSFYSSKNHSVT
Sbjct: 762 SSHQSFYSSKNHSVT 776

BLAST of MS025592 vs. NCBI nr
Match: XP_008466285.1 (PREDICTED: U-box domain-containing protein 52 [Cucumis melo])

HSP 1 Score: 1028.1 bits (2657), Expect = 3.1e-296
Identity = 545/617 (88.33%), Postives = 578/617 (93.68%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGLDFPSKPPQDE 60
           MRSASRPAPT+  LRGQLAQQCS KSD  +F  +   +ARVS++KPP  LD P+K  QDE
Sbjct: 160 MRSASRPAPTVGALRGQLAQQCSTKSDTPDFPVSHSASARVSLDKPP--LDLPNK-SQDE 219

Query: 61  LDFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDNQD-VGRVT-PR 120
            DFM+SPFTRKGYNYK SYGDL M DSDISFVSSGRPSIDRIFP+LY++QD VGR T P+
Sbjct: 220 ADFMRSPFTRKGYNYKPSYGDLPMQDSDISFVSSGRPSIDRIFPTLYESQDMVGRATPPQ 279

Query: 121 MSSSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAMEDVEAEMRRLKL 180
           +SSSTDLDLT SFESL +GRMSLDM+ PSEFSSISQDSDRLSISSQ+MEDVEAEMRRLKL
Sbjct: 280 ISSSTDLDLTRSFESLQLGRMSLDMNFPSEFSSISQDSDRLSISSQSMEDVEAEMRRLKL 339

Query: 181 ELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSKA 240
           ELKQTMEMYSTACKEALTAKQKAVELQRWKLEEE+RLEEARLAGEAALALAEQEKAKSKA
Sbjct: 340 ELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRLEEARLAGEAALALAEQEKAKSKA 399

Query: 241 AIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQND-IRYRKYTIEEIEAAT 300
           A+EAAEAARRIAELEAQKRMKAEM+ALKEAEEKKKA DAL+Q D IRYRKYTIEEIEAAT
Sbjct: 400 AVEAAEAARRIAELEAQKRMKAEMKALKEAEEKKKAFDALAQTDNIRYRKYTIEEIEAAT 459

Query: 301 EFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVL 360
           EFFSESR+IGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVL
Sbjct: 460 EFFSESRRIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVL 519

Query: 361 LLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEP 420
           LLGACPEYGCLVYEFMANGSLEDRL RRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEP
Sbjct: 520 LLGACPEYGCLVYEFMANGSLEDRLLRRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEP 579

Query: 421 LVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQT 480
           LVHRDLKPANILLDRNFVSKISDVGLARLVPPS+AD+VTQYRMTSTAGTFCYIDPEYQQT
Sbjct: 580 LVHRDLKPANILLDRNFVSKISDVGLARLVPPSIADSVTQYRMTSTAGTFCYIDPEYQQT 639

Query: 481 GMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALSF 540
           GMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKG F +ILDP ++DWPVEEALSF
Sbjct: 640 GMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGMFETILDPAITDWPVEEALSF 699

Query: 541 AKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRSHSQVSLQLG 600
           AK+SLKCAELRRKDRPDLGKAILPELNRLR +AEDTMHPTLM GD SPSR++SQVSLQLG
Sbjct: 700 AKLSLKCAELRRKDRPDLGKAILPELNRLRAMAEDTMHPTLM-GDASPSRNNSQVSLQLG 759

Query: 601 SMTSSHQSFYSSKNHSV 615
           +M SS QS+ SS+N SV
Sbjct: 760 AMASSPQSYNSSRNPSV 772

BLAST of MS025592 vs. NCBI nr
Match: KAA0038736.1 (U-box domain-containing protein 52 [Cucumis melo var. makuwa])

HSP 1 Score: 1015.0 bits (2623), Expect = 2.7e-292
Identity = 545/629 (86.65%), Postives = 578/629 (91.89%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAAR-VSIEKPPVGLDFPSKPPQD 60
           MRSASRPAPT+  LRGQLAQQCS KSD  +F  +   +AR VS++KPP  LD P+K  QD
Sbjct: 120 MRSASRPAPTVGALRGQLAQQCSTKSDTPDFPVSHSASARAVSLDKPP--LDLPNK-SQD 179

Query: 61  ELDFM-----------KSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYD 120
           E DFM           +SPFTRKGYNYK SYGDL M DSDISFVSSGRPSIDRIFP+LY+
Sbjct: 180 EADFMRESDEKKCVGSRSPFTRKGYNYKPSYGDLPMQDSDISFVSSGRPSIDRIFPTLYE 239

Query: 121 NQD-VGRVT-PRMSSSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAM 180
           +QD VGR T P++SSSTDLDLT SFESL +GRMSLDM+ PSEFSSISQDSDRLSISSQ+M
Sbjct: 240 SQDMVGRATPPQISSSTDLDLTRSFESLQLGRMSLDMNFPSEFSSISQDSDRLSISSQSM 299

Query: 181 EDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAAL 240
           EDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEE+RLEEARLAGEAAL
Sbjct: 300 EDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRLEEARLAGEAAL 359

Query: 241 ALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQND-IRY 300
           ALAEQEKAKSKAA+EAAEAARRIAELEAQKRMKAEM+ALKEAEEKKKA DAL+Q D IRY
Sbjct: 360 ALAEQEKAKSKAAVEAAEAARRIAELEAQKRMKAEMKALKEAEEKKKAFDALAQTDNIRY 419

Query: 301 RKYTIEEIEAATEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVE 360
           RKYTIEEIEAATEFFSESR+IGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVE
Sbjct: 420 RKYTIEEIEAATEFFSESRRIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVE 479

Query: 361 VLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGT 420
           VLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL RRGNTPPLSWQLRFKIAAEIGT
Sbjct: 480 VLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLRRGNTPPLSWQLRFKIAAEIGT 539

Query: 421 ALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAG 480
           ALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPS+AD+VTQYRMTSTAG
Sbjct: 540 ALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSIADSVTQYRMTSTAG 599

Query: 481 TFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPT 540
           TFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKG F +ILDP 
Sbjct: 600 TFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGMFETILDPA 659

Query: 541 VSDWPVEEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSP 600
           ++DWPVEEALSFAK+SLKCAELRRKDRPDLGKAILPELNRLR +AEDTMHPTLM GD SP
Sbjct: 660 ITDWPVEEALSFAKLSLKCAELRRKDRPDLGKAILPELNRLRAMAEDTMHPTLM-GDASP 719

Query: 601 SRSHSQVSLQLGSMTSSHQSFYSSKNHSV 615
           SR++SQVSLQLG+M SS QS+ SS+N SV
Sbjct: 720 SRNNSQVSLQLGAMASSPQSYNSSRNPSV 744

BLAST of MS025592 vs. NCBI nr
Match: XP_011652539.1 (U-box domain-containing protein 52 [Cucumis sativus] >KGN60182.1 hypothetical protein Csa_001869 [Cucumis sativus])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.3e-289
Identity = 537/617 (87.03%), Postives = 571/617 (92.54%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQLAQQCSAK-SDATEFHSATGTAARVSIEKPPVGLDFPSKPPQD 60
           MRSASRPAPT+S LRG LAQQ S K SD  +F  +    ARVS++KPP  LD P+K  QD
Sbjct: 160 MRSASRPAPTVSALRGPLAQQSSKKLSDTPDFPVSHSATARVSLDKPP--LDLPNK-SQD 219

Query: 61  ELDFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDNQDV-GRVT-P 120
           E D+M+SPFTRKGYNYK SYGDL M DSDISFVSSGRPSIDRIFP+LY++QD+ GR T P
Sbjct: 220 EADYMRSPFTRKGYNYKPSYGDLPMQDSDISFVSSGRPSIDRIFPTLYESQDMGGRATPP 279

Query: 121 RMSSSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAMEDVEAEMRRLK 180
           ++SSSTDLDLT SFESL +GRMS+DM+ PSEFSSISQDSDRLSISSQ+MEDVEAEMRRLK
Sbjct: 280 QISSSTDLDLTRSFESLQLGRMSVDMNFPSEFSSISQDSDRLSISSQSMEDVEAEMRRLK 339

Query: 181 LELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSK 240
           LELKQTMEMYSTACKEALTAKQKAVELQRWKLEEE+RLEEARLAGEAALALAEQEKAKSK
Sbjct: 340 LELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRLEEARLAGEAALALAEQEKAKSK 399

Query: 241 AAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQND-IRYRKYTIEEIEAA 300
           AA+EAAEAARRIAELEAQKRMKAEM+ALKEAEEKKKA DAL+Q D IRYRKYTIEEIEAA
Sbjct: 400 AAVEAAEAARRIAELEAQKRMKAEMKALKEAEEKKKAYDALAQTDNIRYRKYTIEEIEAA 459

Query: 301 TEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMV 360
           TEFFSESR+IGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMV
Sbjct: 460 TEFFSESRRIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMV 519

Query: 361 LLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPE 420
           LLLGACPEYGCLVYEFMANGSLEDRL RRGNTP LSWQLRFKIAAEIGTALLFLHQTKPE
Sbjct: 520 LLLGACPEYGCLVYEFMANGSLEDRLLRRGNTPALSWQLRFKIAAEIGTALLFLHQTKPE 579

Query: 421 PLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQ 480
           PLVHRDLKPANILLDRNFVSKISDVGLARLVPPS+AD+VTQYRMTSTAGTFCYIDPEYQQ
Sbjct: 580 PLVHRDLKPANILLDRNFVSKISDVGLARLVPPSIADSVTQYRMTSTAGTFCYIDPEYQQ 639

Query: 481 TGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALS 540
           TGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVER+IEKG F +ILDP ++DWP+EEALS
Sbjct: 640 TGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERSIEKGMFDTILDPIITDWPIEEALS 699

Query: 541 FAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRSHSQVSLQL 600
           FAK+SLKCAELRRKDRPDLGK ILPELNRLR IAEDTMHPTLM GDTSPSRS SQVS  L
Sbjct: 700 FAKLSLKCAELRRKDRPDLGKVILPELNRLRAIAEDTMHPTLM-GDTSPSRSSSQVSFHL 759

Query: 601 GSMTSSHQSFYSSKNHS 614
           G+M SS QS+ SS+N S
Sbjct: 760 GAMASSPQSYNSSRNPS 772

BLAST of MS025592 vs. NCBI nr
Match: XP_023536596.1 (U-box domain-containing protein 35-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1000.3 bits (2585), Expect = 7.0e-288
Identity = 534/628 (85.03%), Postives = 562/628 (89.49%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGL---------- 60
           MRSASRPAP ISPLRGQLAQQ S KSD  E       +AR S ++PP+ L          
Sbjct: 162 MRSASRPAPVISPLRGQLAQQSSTKSDTPEIQMTHSASARASADRPPLDLPCNLPCNLPC 221

Query: 61  DFPSKPPQDELDFMKSPFTRKGYNYK-ASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDN 120
           + PSK  QDE DFM+SPFTRKGYNYK +SYGDL   +SDISFVSSGRPSIDRIFPSLYD+
Sbjct: 222 NLPSK-VQDETDFMRSPFTRKGYNYKPSSYGDLPTQESDISFVSSGRPSIDRIFPSLYDS 281

Query: 121 QDVG--RVTPRMSSSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAME 180
           QD G     P++SSSTD DL HSFESL MGRMS DM+  SEFSSISQDSDRLSISSQ+ME
Sbjct: 282 QDTGGRSTPPQVSSSTDFDLPHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSME 341

Query: 181 DVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALA 240
           DVEAEMRRLKLELKQTMEMYSTACKEALTAKQK VELQRWKLEEE+RLEEARLAGEAALA
Sbjct: 342 DVEAEMRRLKLELKQTMEMYSTACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALA 401

Query: 241 LAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRYRK 300
           LAEQEK KSKAAIEAAEAARRIAELE QKRMKAEM+ALKEAEEKKKAVDAL+ NDIRYRK
Sbjct: 402 LAEQEKLKSKAAIEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRK 461

Query: 301 YTIEEIEAATEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVL 360
           YTI+EIE ATEFF+ESRKIGEGGYGPVY+CDLDHTPVAIKVLRPDATQGRSQFKQEVEVL
Sbjct: 462 YTIDEIEVATEFFAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQEVEVL 521

Query: 361 SCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTAL 420
           SCIRHPNMVLLLGACPEYGCLVYE+MANGSLEDRL RRGNTPPLSWQLRFKIAAEIGTAL
Sbjct: 522 SCIRHPNMVLLLGACPEYGCLVYEYMANGSLEDRLLRRGNTPPLSWQLRFKIAAEIGTAL 581

Query: 421 LFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTF 480
           LFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPS+A++VTQYRMTSTAGTF
Sbjct: 582 LFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTF 641

Query: 481 CYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVS 540
           CYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIE GTF +ILDP V 
Sbjct: 642 CYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENGTFETILDPAVL 701

Query: 541 DWPVEEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSR 600
           DWP+EEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDT HPTLMGGD SPS 
Sbjct: 702 DWPIEEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTTHPTLMGGDISPSC 761

Query: 601 SHSQVSLQLGSMTSSHQSFYSSKNHSVT 616
           S+SQVSLQ G   SS QS+ SS+N S T
Sbjct: 762 SNSQVSLQFG---SSPQSYNSSRNPSTT 785

BLAST of MS025592 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 3.1e-105
Identity = 212/435 (48.74%), Postives = 281/435 (64.60%), Query Frame = 0

Query: 142 DMSCPSEFSSIS------QDSDR----LSISSQAMEDVEAEMRRLKLELKQTMEMYSTAC 201
           D +C    S++       +DSDR     S  S+ +E +E E+ RLK EL+ T+  Y  AC
Sbjct: 275 DKTCTKPQSNVENIVSEHRDSDRSPPETSRKSKKVE-IEEEVERLKNELQSTVFKYKQAC 334

Query: 202 KEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAE 261
           +E  + + K   L    L E +R+  A    E     A  EK +   A++  E A+ +  
Sbjct: 335 EELFSTQNKVKMLSTEYLNESKRVNNAVEKEELQRNTAALEKERYMKAVKEVETAKALLA 394

Query: 262 LEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRYRKYTIEEIEAATEFFSESRKIGEGGY 321
            E  +R  AE+ AL+   EKKK +D L   D RYRKYTIEEI  ATE FS  + IGEGGY
Sbjct: 395 REFCQRQIAEVNALRTYLEKKKVIDQLLGTDHRYRKYTIEEIVTATEGFSPEKVIGEGGY 454

Query: 322 GPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYE 381
           G VY+C LD TP A+KV+R D  + + +F +EVEVLS +RHP++VLLLGACPE GCLVYE
Sbjct: 455 GKVYQCSLDSTPAAVKVVRLDTPEKKQEFLKEVEVLSQLRHPHVVLLLGACPENGCLVYE 514

Query: 382 FMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVHRDLKPANILLD 441
           ++ NGSLE+ +  R N PPL W +RF++  E+   L FLH +KPEP+VHRDLKP NILL+
Sbjct: 515 YLENGSLEEYIFHRKNKPPLPWFIRFRVIFEVACGLAFLHSSKPEPIVHRDLKPGNILLN 574

Query: 442 RNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGI 501
           RN+VSKI+DVGLA+LV     DNVT YR +  AGT  YIDPEY +TG +  KSD+Y+ GI
Sbjct: 575 RNYVSKIADVGLAKLVTDVAPDNVTMYRNSVLAGTLHYIDPEYHRTGTIRPKSDLYAFGI 634

Query: 502 MFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALSFAKMSLKCAELRRKD 561
           + LQL+T+R P G+   VE A++KGT   +LD +V+DWP+ E    A++ LKCAE R +D
Sbjct: 635 IILQLLTARNPSGIVPAVENAVKKGTLTEMLDKSVTDWPLAETEELARIGLKCAEFRCRD 694

Query: 562 RPDLGKAILPELNRL 567
           RPDL   ++P L RL
Sbjct: 695 RPDLKSEVIPVLKRL 708

BLAST of MS025592 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 379.0 bits (972), Expect = 1.0e-103
Identity = 222/482 (46.06%), Postives = 293/482 (60.79%), Query Frame = 0

Query: 109 NQDVGRVTPRMS-SSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAME 168
           N++ G    R S S   +D THS  S     M                SD LS  S    
Sbjct: 302 NREYGNFGTRFSWSGMGVDTTHSRASQQASNM----------------SDALSEQSYTDN 361

Query: 169 DVEA--EMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAA 228
            V    E+ +L+ EL+   EMY+ A  E   A +K  EL + +LEE  +LEE +L    A
Sbjct: 362 QVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGELNQRRLEEAIKLEELKLKEYEA 421

Query: 229 LALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRY 288
             LAE+EK   + A   AE+ R  AE E  +R +AE ++ ++ +EK+K    L    ++Y
Sbjct: 422 RELAEKEKQNFEKARRDAESMRERAEREIAQRREAERKSARDTKEKEKLEGTLGSPQLQY 481

Query: 289 RKYTIEEIEAATEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVE 348
           + +  EEI AAT  FSE  KIG G YG VYKC+L HT   +KVL+    Q   QF+QE+E
Sbjct: 482 QHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTTAVVKVLQSAENQLSKQFQQELE 541

Query: 349 VLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGT 408
           +LS IRHP++VLLLGACPE G LVYE+M NGSLEDRL +  N+PPL W  RF+IA E+  
Sbjct: 542 ILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQVNNSPPLPWFERFRIAWEVAA 601

Query: 409 ALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVP-PSVADNVTQYRMTSTA 468
           AL+FLH++KP+P++HRDLKPANILLD NFVSK+ DVGL+ +V    ++   T Y+ TS  
Sbjct: 602 ALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTSPV 661

Query: 469 GTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKG-TFASILD 528
           GT CYIDPEYQ+TG +  KSDIYS G++ LQL+T++P + LTH VE A++    F  ILD
Sbjct: 662 GTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKPAIALTHFVESAMDSNDEFLKILD 721

Query: 529 PTVSDWPVEEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDT 586
               +WP+EE    A ++L C ELR KDRPDL   ILP L  L+ +AE   + +  G  T
Sbjct: 722 QKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILPALENLKKVAEKARN-SFSGVST 766

BLAST of MS025592 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.9e-102
Identity = 219/500 (43.80%), Postives = 307/500 (61.40%), Query Frame = 0

Query: 108 DNQDVGRVTPRMSSSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLS---ISSQ 167
           D++D  R   R SSS++    H   ++  G +      P  +S +S  +  +S   +S  
Sbjct: 301 DHED--RREMRSSSSSN---NHDLVNMDWGAV-----VPENYSWVSHTASHMSDGLLSVH 360

Query: 168 AMED----VEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARL 227
           ++ D    +  E+ +L+ ELK   EMY+ A  E + A +K  EL + + EE  +L E + 
Sbjct: 361 SITDNQVNLNFEIEKLRAELKHVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKE 420

Query: 228 AGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQ 287
             E A   A +EK + + A++ AE  + +   EA  R +AE +A ++A EK K   +L  
Sbjct: 421 KEEVAKDTASKEKQRYEEAMKEAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVS 480

Query: 288 NDIRYRKYTIEEIEAATEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQF 347
             ++Y+ YT EEI AAT  F+E+ KIG G YG VYKC+L HT  A+KVL    TQ   QF
Sbjct: 481 PGVQYQHYTWEEIAAATSDFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQF 540

Query: 348 KQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIA 407
            QE+E+LS IRHP++VLLLGACPE GCLVYE+M NGSL+DRL    +TPP+ W  RF+IA
Sbjct: 541 DQELEILSKIRHPHLVLLLGACPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIA 600

Query: 408 AEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRM 467
            E+ +AL+FLH++KP P++HRDLKP NILLD NFVSK+ DVGL+ +V      + T ++ 
Sbjct: 601 LEVASALVFLHKSKPRPIIHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQ 660

Query: 468 TSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAI-EKGTFA 527
           TS  GT CYIDPEYQ+TG++  KSD+YSLG++ LQLIT++P + +TH VE AI +   F 
Sbjct: 661 TSPVGTLCYIDPEYQRTGIISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFM 720

Query: 528 SILDPTVSDWPVEEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLM 587
           +ILD     WP+ +    A + L C E+RR+DRPDL   I+P L RLR +A+   +  L+
Sbjct: 721 AILDKKAGSWPISDTRELAALGLCCTEMRRRDRPDLKDQIIPALERLRKVADKAQN--LL 780

Query: 588 GGDTSPSRSHSQVSLQLGSM 600
               S   SH    L  G M
Sbjct: 781 SRTPSGPPSHFICPLLKGVM 788

BLAST of MS025592 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 370.5 bits (950), Expect = 3.6e-101
Identity = 212/434 (48.85%), Postives = 281/434 (64.75%), Query Frame = 0

Query: 148 EFSSISQDSDRLSISSQAMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRW 207
           E SS S  SD  S SSQ  +D E E  +LK+EL+    MY+ A  E + A +K  +L + 
Sbjct: 276 EASSSSTYSDPTSSSSQIHKDFELE--KLKIELRHIKGMYAVAQSEVIDASKKMQDLNQR 335

Query: 208 KLEEERRLEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMRALKE 267
           + EE  RL+   +  E A  + E E+ + + A   AE  R   E E ++R++AE RA + 
Sbjct: 336 RSEEATRLKNLTIREEEADEVVEMERERQEDAENEAELVRECIERETEERLEAEARAEEV 395

Query: 268 AEEKKKAVDALSQNDI---RYRKYTIEEIEAATEFFSESRKIGEGGYGPVYKCDLDHTPV 327
            +EK++  DAL    +   +Y K+  EEI  AT  FS+  KIG GGYG VY+C+L HT V
Sbjct: 396 RKEKQRLEDALEGGPLQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRCNLHHTTV 455

Query: 328 AIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLAR 387
           A+KVL  D +    QF QE+E+LS IRHP+++LLLGACPE G LVYE+M NGSLE+RL +
Sbjct: 456 AVKVLHSDKSSLTKQFHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGSLEERLMK 515

Query: 388 R------GNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKI 447
           R         PPL W  RF+IA EI +AL FLH  +P P+VHRDLKPANILLDRN VSKI
Sbjct: 516 RRPNVDTPQPPPLRWFERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLDRNNVSKI 575

Query: 448 SDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLIT 507
            DVGL+++V    +   T +  T   GTF YIDPEYQ+TG++  +SDIY+ GI+ LQL+T
Sbjct: 576 GDVGLSKMVNLDPSHASTVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGIILLQLVT 635

Query: 508 SRPPMGLTHHVERAI--EKGTFASILDPTVSDWPVEEALSFAKMSLKCAELRRKDRPDLG 567
           +R  MGL H +E+A+  + G F  ILD T  DWPV+EA     + L+CAE+R++DRPDLG
Sbjct: 636 ARSAMGLAHSIEKALRDQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRKRDRPDLG 695

Query: 568 KAILPELNRLRTIA 571
           K ILP L RL+ +A
Sbjct: 696 KEILPVLERLKEVA 707

BLAST of MS025592 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 5.7e-91
Identity = 193/426 (45.31%), Postives = 265/426 (62.21%), Query Frame = 0

Query: 167 EDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAAL 226
           +D   E+ +L+ EL+   EMY+ A  E L A +K  EL   K EE   LE          
Sbjct: 343 QDTLNEISKLRAELRHAHEMYAVAQVETLDASRKLNEL---KFEELTLLE---------- 402

Query: 227 ALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVD-ALSQNDIRY 286
               + K  +K   E  E  RR  E EA +R +AEM+A  EA+EK+K  + +L    ++Y
Sbjct: 403 ---HETKGIAKKETEKFEQKRR-EEREAAQRREAEMKATHEAKEKEKLEESSLVAPKLQY 462

Query: 287 RKYTIEEIEAATEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVE 346
           +++T EEI  AT  FSE  KIG G YG VYKC+L HT  A+KVL    +    QF QE+E
Sbjct: 463 QEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKVLHSAESSLSKQFDQELE 522

Query: 347 VLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGT 406
           +LS IRHP++VLLLGACP++G LVYE+M NGSLEDRL +  ++ P+ W +R +IA E+ +
Sbjct: 523 ILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDSQPIPWFVRLRIAWEVAS 582

Query: 407 ALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPS--VADNVTQYRMTST 466
           AL+FLH++KP P++HRDLKPANILL+ NFVSK+ DVGL+ ++  +  ++   T Y+ TS 
Sbjct: 583 ALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQAADPLSTKFTMYKQTSP 642

Query: 467 AGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGT---FAS 526
            GT CYIDPEYQ+TG +  KSD+Y+ G++ LQL+T +  M LT+ VE A+E         
Sbjct: 643 VGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAMALTYTVETAMENNNDDELIQ 702

Query: 527 ILDPTVSDWPVEEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMG 586
           ILD    +WP+EE    A ++L+C ELR KDRPDL   ILP L  L+ +A+   +     
Sbjct: 703 ILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLEDQILPVLESLKKVADKARNSLSAA 751

BLAST of MS025592 vs. ExPASy TrEMBL
Match: A0A6J1CTA9 (U-box domain-containing protein 52-like OS=Momordica charantia OX=3673 GN=LOC111014066 PE=4 SV=1)

HSP 1 Score: 1184.9 bits (3064), Expect = 0.0e+00
Identity = 615/615 (100.00%), Postives = 615/615 (100.00%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGLDFPSKPPQDE 60
           MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGLDFPSKPPQDE
Sbjct: 162 MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGLDFPSKPPQDE 221

Query: 61  LDFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDNQDVGRVTPRMS 120
           LDFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDNQDVGRVTPRMS
Sbjct: 222 LDFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDNQDVGRVTPRMS 281

Query: 121 SSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAMEDVEAEMRRLKLEL 180
           SSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAMEDVEAEMRRLKLEL
Sbjct: 282 SSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAMEDVEAEMRRLKLEL 341

Query: 181 KQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSKAAI 240
           KQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSKAAI
Sbjct: 342 KQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSKAAI 401

Query: 241 EAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRYRKYTIEEIEAATEFF 300
           EAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRYRKYTIEEIEAATEFF
Sbjct: 402 EAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRYRKYTIEEIEAATEFF 461

Query: 301 SESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLG 360
           SESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLG
Sbjct: 462 SESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLG 521

Query: 361 ACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVH 420
           ACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVH
Sbjct: 522 ACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVH 581

Query: 421 RDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGML 480
           RDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGML
Sbjct: 582 RDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGML 641

Query: 481 GVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALSFAKM 540
           GVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALSFAKM
Sbjct: 642 GVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALSFAKM 701

Query: 541 SLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRSHSQVSLQLGSMT 600
           SLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRSHSQVSLQLGSMT
Sbjct: 702 SLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRSHSQVSLQLGSMT 761

Query: 601 SSHQSFYSSKNHSVT 616
           SSHQSFYSSKNHSVT
Sbjct: 762 SSHQSFYSSKNHSVT 776

BLAST of MS025592 vs. ExPASy TrEMBL
Match: A0A1S3CQW0 (U-box domain-containing protein 52 OS=Cucumis melo OX=3656 GN=LOC103503739 PE=4 SV=1)

HSP 1 Score: 1028.1 bits (2657), Expect = 1.5e-296
Identity = 545/617 (88.33%), Postives = 578/617 (93.68%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGLDFPSKPPQDE 60
           MRSASRPAPT+  LRGQLAQQCS KSD  +F  +   +ARVS++KPP  LD P+K  QDE
Sbjct: 160 MRSASRPAPTVGALRGQLAQQCSTKSDTPDFPVSHSASARVSLDKPP--LDLPNK-SQDE 219

Query: 61  LDFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDNQD-VGRVT-PR 120
            DFM+SPFTRKGYNYK SYGDL M DSDISFVSSGRPSIDRIFP+LY++QD VGR T P+
Sbjct: 220 ADFMRSPFTRKGYNYKPSYGDLPMQDSDISFVSSGRPSIDRIFPTLYESQDMVGRATPPQ 279

Query: 121 MSSSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAMEDVEAEMRRLKL 180
           +SSSTDLDLT SFESL +GRMSLDM+ PSEFSSISQDSDRLSISSQ+MEDVEAEMRRLKL
Sbjct: 280 ISSSTDLDLTRSFESLQLGRMSLDMNFPSEFSSISQDSDRLSISSQSMEDVEAEMRRLKL 339

Query: 181 ELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSKA 240
           ELKQTMEMYSTACKEALTAKQKAVELQRWKLEEE+RLEEARLAGEAALALAEQEKAKSKA
Sbjct: 340 ELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRLEEARLAGEAALALAEQEKAKSKA 399

Query: 241 AIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQND-IRYRKYTIEEIEAAT 300
           A+EAAEAARRIAELEAQKRMKAEM+ALKEAEEKKKA DAL+Q D IRYRKYTIEEIEAAT
Sbjct: 400 AVEAAEAARRIAELEAQKRMKAEMKALKEAEEKKKAFDALAQTDNIRYRKYTIEEIEAAT 459

Query: 301 EFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVL 360
           EFFSESR+IGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVL
Sbjct: 460 EFFSESRRIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVL 519

Query: 361 LLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEP 420
           LLGACPEYGCLVYEFMANGSLEDRL RRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEP
Sbjct: 520 LLGACPEYGCLVYEFMANGSLEDRLLRRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEP 579

Query: 421 LVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQT 480
           LVHRDLKPANILLDRNFVSKISDVGLARLVPPS+AD+VTQYRMTSTAGTFCYIDPEYQQT
Sbjct: 580 LVHRDLKPANILLDRNFVSKISDVGLARLVPPSIADSVTQYRMTSTAGTFCYIDPEYQQT 639

Query: 481 GMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALSF 540
           GMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKG F +ILDP ++DWPVEEALSF
Sbjct: 640 GMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGMFETILDPAITDWPVEEALSF 699

Query: 541 AKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRSHSQVSLQLG 600
           AK+SLKCAELRRKDRPDLGKAILPELNRLR +AEDTMHPTLM GD SPSR++SQVSLQLG
Sbjct: 700 AKLSLKCAELRRKDRPDLGKAILPELNRLRAMAEDTMHPTLM-GDASPSRNNSQVSLQLG 759

Query: 601 SMTSSHQSFYSSKNHSV 615
           +M SS QS+ SS+N SV
Sbjct: 760 AMASSPQSYNSSRNPSV 772

BLAST of MS025592 vs. ExPASy TrEMBL
Match: A0A5A7T956 (U-box domain-containing protein 52 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G002830 PE=4 SV=1)

HSP 1 Score: 1015.0 bits (2623), Expect = 1.3e-292
Identity = 545/629 (86.65%), Postives = 578/629 (91.89%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAAR-VSIEKPPVGLDFPSKPPQD 60
           MRSASRPAPT+  LRGQLAQQCS KSD  +F  +   +AR VS++KPP  LD P+K  QD
Sbjct: 120 MRSASRPAPTVGALRGQLAQQCSTKSDTPDFPVSHSASARAVSLDKPP--LDLPNK-SQD 179

Query: 61  ELDFM-----------KSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYD 120
           E DFM           +SPFTRKGYNYK SYGDL M DSDISFVSSGRPSIDRIFP+LY+
Sbjct: 180 EADFMRESDEKKCVGSRSPFTRKGYNYKPSYGDLPMQDSDISFVSSGRPSIDRIFPTLYE 239

Query: 121 NQD-VGRVT-PRMSSSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAM 180
           +QD VGR T P++SSSTDLDLT SFESL +GRMSLDM+ PSEFSSISQDSDRLSISSQ+M
Sbjct: 240 SQDMVGRATPPQISSSTDLDLTRSFESLQLGRMSLDMNFPSEFSSISQDSDRLSISSQSM 299

Query: 181 EDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAAL 240
           EDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEE+RLEEARLAGEAAL
Sbjct: 300 EDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRLEEARLAGEAAL 359

Query: 241 ALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQND-IRY 300
           ALAEQEKAKSKAA+EAAEAARRIAELEAQKRMKAEM+ALKEAEEKKKA DAL+Q D IRY
Sbjct: 360 ALAEQEKAKSKAAVEAAEAARRIAELEAQKRMKAEMKALKEAEEKKKAFDALAQTDNIRY 419

Query: 301 RKYTIEEIEAATEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVE 360
           RKYTIEEIEAATEFFSESR+IGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVE
Sbjct: 420 RKYTIEEIEAATEFFSESRRIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVE 479

Query: 361 VLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGT 420
           VLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL RRGNTPPLSWQLRFKIAAEIGT
Sbjct: 480 VLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLRRGNTPPLSWQLRFKIAAEIGT 539

Query: 421 ALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAG 480
           ALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPS+AD+VTQYRMTSTAG
Sbjct: 540 ALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSIADSVTQYRMTSTAG 599

Query: 481 TFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPT 540
           TFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKG F +ILDP 
Sbjct: 600 TFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGMFETILDPA 659

Query: 541 VSDWPVEEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSP 600
           ++DWPVEEALSFAK+SLKCAELRRKDRPDLGKAILPELNRLR +AEDTMHPTLM GD SP
Sbjct: 660 ITDWPVEEALSFAKLSLKCAELRRKDRPDLGKAILPELNRLRAMAEDTMHPTLM-GDASP 719

Query: 601 SRSHSQVSLQLGSMTSSHQSFYSSKNHSV 615
           SR++SQVSLQLG+M SS QS+ SS+N SV
Sbjct: 720 SRNNSQVSLQLGAMASSPQSYNSSRNPSV 744

BLAST of MS025592 vs. ExPASy TrEMBL
Match: A0A0A0LHG8 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G882930 PE=4 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 6.2e-290
Identity = 537/617 (87.03%), Postives = 571/617 (92.54%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQLAQQCSAK-SDATEFHSATGTAARVSIEKPPVGLDFPSKPPQD 60
           MRSASRPAPT+S LRG LAQQ S K SD  +F  +    ARVS++KPP  LD P+K  QD
Sbjct: 160 MRSASRPAPTVSALRGPLAQQSSKKLSDTPDFPVSHSATARVSLDKPP--LDLPNK-SQD 219

Query: 61  ELDFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDNQDV-GRVT-P 120
           E D+M+SPFTRKGYNYK SYGDL M DSDISFVSSGRPSIDRIFP+LY++QD+ GR T P
Sbjct: 220 EADYMRSPFTRKGYNYKPSYGDLPMQDSDISFVSSGRPSIDRIFPTLYESQDMGGRATPP 279

Query: 121 RMSSSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAMEDVEAEMRRLK 180
           ++SSSTDLDLT SFESL +GRMS+DM+ PSEFSSISQDSDRLSISSQ+MEDVEAEMRRLK
Sbjct: 280 QISSSTDLDLTRSFESLQLGRMSVDMNFPSEFSSISQDSDRLSISSQSMEDVEAEMRRLK 339

Query: 181 LELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSK 240
           LELKQTMEMYSTACKEALTAKQKAVELQRWKLEEE+RLEEARLAGEAALALAEQEKAKSK
Sbjct: 340 LELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRLEEARLAGEAALALAEQEKAKSK 399

Query: 241 AAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQND-IRYRKYTIEEIEAA 300
           AA+EAAEAARRIAELEAQKRMKAEM+ALKEAEEKKKA DAL+Q D IRYRKYTIEEIEAA
Sbjct: 400 AAVEAAEAARRIAELEAQKRMKAEMKALKEAEEKKKAYDALAQTDNIRYRKYTIEEIEAA 459

Query: 301 TEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMV 360
           TEFFSESR+IGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMV
Sbjct: 460 TEFFSESRRIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMV 519

Query: 361 LLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPE 420
           LLLGACPEYGCLVYEFMANGSLEDRL RRGNTP LSWQLRFKIAAEIGTALLFLHQTKPE
Sbjct: 520 LLLGACPEYGCLVYEFMANGSLEDRLLRRGNTPALSWQLRFKIAAEIGTALLFLHQTKPE 579

Query: 421 PLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQ 480
           PLVHRDLKPANILLDRNFVSKISDVGLARLVPPS+AD+VTQYRMTSTAGTFCYIDPEYQQ
Sbjct: 580 PLVHRDLKPANILLDRNFVSKISDVGLARLVPPSIADSVTQYRMTSTAGTFCYIDPEYQQ 639

Query: 481 TGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALS 540
           TGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVER+IEKG F +ILDP ++DWP+EEALS
Sbjct: 640 TGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERSIEKGMFDTILDPIITDWPIEEALS 699

Query: 541 FAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRSHSQVSLQL 600
           FAK+SLKCAELRRKDRPDLGK ILPELNRLR IAEDTMHPTLM GDTSPSRS SQVS  L
Sbjct: 700 FAKLSLKCAELRRKDRPDLGKVILPELNRLRAIAEDTMHPTLM-GDTSPSRSSSQVSFHL 759

Query: 601 GSMTSSHQSFYSSKNHS 614
           G+M SS QS+ SS+N S
Sbjct: 760 GAMASSPQSYNSSRNPS 772

BLAST of MS025592 vs. ExPASy TrEMBL
Match: A0A6J1FEZ3 (U-box domain-containing protein 52-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443340 PE=4 SV=1)

HSP 1 Score: 996.1 bits (2574), Expect = 6.4e-287
Identity = 532/636 (83.65%), Postives = 563/636 (88.52%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGL---------- 60
           MRSASRPAP ISPLR QLAQQ S KSD  E       +AR S ++PP+ L          
Sbjct: 162 MRSASRPAPVISPLRSQLAQQSSTKSDTPEIQMTHSASARASADRPPLDLPCNLPCNLPC 221

Query: 61  --------DFPSKPPQDELDFMKSPFTRKGYNYK-ASYGDLSMHDSDISFVSSGRPSIDR 120
                   + PSK  QD+ DFM+SPFTRKGYNYK +SYGDL   +SDISFVSSGRPSIDR
Sbjct: 222 NLPCNLPCNLPSK-VQDDTDFMRSPFTRKGYNYKPSSYGDLPTQESDISFVSSGRPSIDR 281

Query: 121 IFPSLYDNQDVG--RVTPRMSSSTDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRL 180
           IFPSLYD+QD G     P++SSSTD DL HSFESL MGRMS DM+  SEFSSISQDSDRL
Sbjct: 282 IFPSLYDSQDTGGRSTPPQVSSSTDFDLPHSFESLQMGRMSADMNFSSEFSSISQDSDRL 341

Query: 181 SISSQAMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEAR 240
           SISSQ+MEDVEAEMRRL+LELKQTMEMYSTACKEALTAKQKAVELQRWKLEEE+RLEEAR
Sbjct: 342 SISSQSMEDVEAEMRRLRLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRLEEAR 401

Query: 241 LAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALS 300
           LAGEAALALAEQEK KSKAAIEAAEAARRIAELE QKRMKAEM+ALKEAEEKKKAVDAL+
Sbjct: 402 LAGEAALALAEQEKLKSKAAIEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALA 461

Query: 301 QNDIRYRKYTIEEIEAATEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQ 360
            NDIRYRKYTI+EIE ATEFF+ESRKIGEGGYGPVY+CDLDHTPVAIKVLRPDATQGRSQ
Sbjct: 462 NNDIRYRKYTIDEIEVATEFFAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQ 521

Query: 361 FKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKI 420
           FKQEVEVLSCIRHPNMVLLLGACPEYGCLVYE+MANGSLEDRL RRGNTPPLSWQLRFKI
Sbjct: 522 FKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEYMANGSLEDRLLRRGNTPPLSWQLRFKI 581

Query: 421 AAEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYR 480
           AAEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPS+A++VTQYR
Sbjct: 582 AAEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYR 641

Query: 481 MTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFA 540
           MTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIE GTF 
Sbjct: 642 MTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENGTFE 701

Query: 541 SILDPTVSDWPVEEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLM 600
           +ILDP V DWP++EALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDT HPTLM
Sbjct: 702 TILDPAVLDWPIQEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTTHPTLM 761

Query: 601 GGDTSPSRSHSQVSLQLGSMTSSHQSFYSSKNHSVT 616
           GGD SPSRS+SQVSLQ G   SS QS+ SS+N S T
Sbjct: 762 GGDISPSRSNSQVSLQFG---SSPQSYNSSRNPSTT 793

BLAST of MS025592 vs. TAIR 10
Match: AT2G24370.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 797.0 bits (2057), Expect = 1.1e-230
Identity = 427/620 (68.87%), Postives = 500/620 (80.65%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQL-------------AQQCSAKSDATEFHS----ATGTAARVSI 60
           MRSASR AP  +PLR  +                 S ++D   F S    +     R S+
Sbjct: 176 MRSASRSAPMTAPLRSSVQPPSLKPPQPMPSTSANSLRADRRSFESNQRRSVEDQQRRSM 235

Query: 61  EKPPVGLDFPSKPPQDELDFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIF 120
           E      D   +   D+ +  +SPFTR+G     SYGDL++ +SDISF+SSGRPSIDRIF
Sbjct: 236 E------DQQRRSIDDQSESFRSPFTRRGNG--RSYGDLTVPESDISFISSGRPSIDRIF 295

Query: 121 PSLYDNQDVGRVTPRMSSSTDLDLTHSF-ESLHMGRMSLDMSCPSEFSSISQDSDRLSIS 180
           PSLYDN D  R  PR+S+ +D+D + S  +S + GR S+DM+ P++F     +S+R S +
Sbjct: 296 PSLYDNNDPSRTPPRLSNFSDMDYSSSLDQSSNYGRRSVDMNSPTDF-----ESERFSSA 355

Query: 181 SQAMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAG 240
           SQ+++DVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA ELQRWKLEEER+LEEAR A 
Sbjct: 356 SQSIDDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKATELQRWKLEEERKLEEARNAE 415

Query: 241 EAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQND 300
           EAALA+AE+EKAKSKAA+EAAEAA+RIAELEA+KR+ AEM+ALKE+EEK KA+ AL+ +D
Sbjct: 416 EAALAIAEKEKAKSKAAMEAAEAAQRIAELEAKKRVNAEMKALKESEEKTKALTALANSD 475

Query: 301 IRYRKYTIEEIEAATEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQ 360
           +RYRKY+IE+IE ATEFF+E  KIGEGGYGPVYKC LDHTPVA+KVLRPDA QGRSQF+Q
Sbjct: 476 VRYRKYSIEDIELATEFFAEKYKIGEGGYGPVYKCYLDHTPVAVKVLRPDAAQGRSQFQQ 535

Query: 361 EVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAE 420
           EVEVLSCIRHPNMVLLLGACPE GCLVYEFMANGSLEDRL R GN+PPLSWQ+RF+IAAE
Sbjct: 536 EVEVLSCIRHPNMVLLLGACPECGCLVYEFMANGSLEDRLFRLGNSPPLSWQMRFRIAAE 595

Query: 421 IGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTS 480
           IGT LLFLHQ KPEPLVHRDLKP NILLDRNFVSKISDVGLARLVPP+VAD VTQYRMTS
Sbjct: 596 IGTGLLFLHQAKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPPTVADTVTQYRMTS 655

Query: 481 TAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASIL 540
           TAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLIT++PPMGLTH+VERA+EKGT   +L
Sbjct: 656 TAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITAKPPMGLTHYVERALEKGTLVDLL 715

Query: 541 DPTVSDWPVEEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGD 600
           DP VSDWP+E+   FAK++LKCAELRRKDRPDL K ILPELNRLRT+A+++ H  ++G  
Sbjct: 716 DPVVSDWPMEDTEEFAKLALKCAELRRKDRPDLAKVILPELNRLRTLADESSHSVVVGNS 775

Query: 601 TSPSRSHSQVSLQLGSMTSS 603
             PS + SQ SL+L  M+ +
Sbjct: 776 PLPSPTGSQSSLKLEHMSGA 782

BLAST of MS025592 vs. TAIR 10
Match: AT4G31230.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 747.7 bits (1929), Expect = 7.6e-216
Identity = 398/592 (67.23%), Postives = 474/592 (80.07%), Query Frame = 0

Query: 2   RSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGLDFPSKPPQDEL 61
           ++ASR AP++SPLR QL Q            +    A R S E          +   D+ 
Sbjct: 178 KTASRAAPSVSPLRIQLQQNGLKPHPPLPSATTNTRAERQSFESQ------HRRSLDDQS 237

Query: 62  DFMKSPFTRKGYNYKASYGDLSMHDSDISFVSSGRPSIDRIFPSLYDNQDVGRVTPRMSS 121
           D  + P+ ++G   + SYG+LS+ DS+ISF SSGRPSI+R  PSLYDN D  R  PR+S+
Sbjct: 238 DSFRPPYNKRGLTGR-SYGELSIPDSEISFNSSGRPSIERNSPSLYDNSDPNRTPPRLSN 297

Query: 122 STDLDLTHSFESLHMGRMSLDMSCPSEFSSISQDSDRLSISSQAMEDVEAEMRRLKLELK 181
            +D+D   SFES+  GR S+D+S P+ FS+ S +++R S +SQ  +DVEAEMRRLKLELK
Sbjct: 298 FSDVDYC-SFESMTFGRRSMDLSSPTAFSTGSFENERFSSASQGGDDVEAEMRRLKLELK 357

Query: 182 QTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALALAEQEKAKSKAAIE 241
           QTMEMYSTACKEALTAK KA ELQRWKL EER+ EEA+LA EAALA+AE+EKAKSKAA+E
Sbjct: 358 QTMEMYSTACKEALTAKHKATELQRWKLAEERKFEEAKLAEEAALAIAEKEKAKSKAAME 417

Query: 242 AAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRYRKYTIEEIEAATEFFS 301
           AAEAA+RIA++E++KR+ AE +ALKE+E + KAV+AL++ D+RYRKY+IEEIE ATEFF 
Sbjct: 418 AAEAAQRIADIESRKRVDAETKALKESEARTKAVNALAK-DVRYRKYSIEEIEDATEFFD 477

Query: 302 ESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGA 361
           +  KIGEG YGPVYKC LDHTPVA+K LRPDA QGRSQF++EVEVL  IRHPNMVLLLGA
Sbjct: 478 DKYKIGEGSYGPVYKCYLDHTPVAVKALRPDAAQGRSQFQKEVEVLCSIRHPNMVLLLGA 537

Query: 362 CPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVHR 421
           CPE GCLVYEFMANGSLEDRL R+G++P LSWQ RF+IAAEIGT LLFLHQTKPEPLVHR
Sbjct: 538 CPECGCLVYEFMANGSLEDRLFRQGDSPALSWQTRFRIAAEIGTVLLFLHQTKPEPLVHR 597

Query: 422 DLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLG 481
           DLKPANILLDRNFVSK++DVGLARLVPPSVA+ VTQY MTSTAGTFCYIDPEYQQTGMLG
Sbjct: 598 DLKPANILLDRNFVSKLADVGLARLVPPSVANTVTQYHMTSTAGTFCYIDPEYQQTGMLG 657

Query: 482 VKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSDWPVEEALSFAKMS 541
           VKSDIYSLGIMFLQLIT +PPMGLTH+VERA+EKG    +LDP VSDWPVE+   FAK++
Sbjct: 658 VKSDIYSLGIMFLQLITGKPPMGLTHYVERALEKGNLKDLLDPAVSDWPVEDTTEFAKLA 717

Query: 542 LKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRSHSQVS 594
           LKCAE+RRKDRPDL K ILPELNRLR +AE++    ++   + P+ + SQ S
Sbjct: 718 LKCAEIRRKDRPDLSKVILPELNRLRVLAEESTQAAVVINSSGPTPTESQTS 760

BLAST of MS025592 vs. TAIR 10
Match: AT1G78940.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 642.9 bits (1657), Expect = 2.6e-184
Identity = 360/606 (59.41%), Postives = 461/606 (76.07%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGLDFPSKPPQDE 60
           +R+ASRPAP  + +     QQC    +    H  T   A    + P      PS+ P+  
Sbjct: 92  VRNASRPAPYQNSM-----QQC----EIDNHHPHTPDKAPKYHDHPNSAGSTPSR-PRKS 151

Query: 61  LDFMKSPFT-RKGY-NYKASYGDLSM------HDSDISFVSSGRPSIDRIFPSLYDNQDV 120
           ++  +SP   RK Y ++  S  DLS        DSDISF+SSGRPS++R   SL D  + 
Sbjct: 152 VEADRSPLVKRKPYGDFYDSDSDLSFISPSSHRDSDISFISSGRPSVERSSFSL-DFPES 211

Query: 121 GRVTPRMSSSTDLDLTHSFESLHMGRMSLDMSCP---SEFSSISQDSDRL-SISSQAMED 180
            R T RMS+S++       +S+   R+ +  S P   +E S+ S++S R  S SSQ+++D
Sbjct: 212 AR-TSRMSTSSE-------QSIGSNRLGIKFSDPDFLNESSTFSEESGRTSSYSSQSLDD 271

Query: 181 VEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALAL 240
           VEAEM+RL+LELKQTM+MYSTACKEAL+A+Q+A ELQ+ + EEERRLEEA+ + EAA+++
Sbjct: 272 VEAEMKRLRLELKQTMDMYSTACKEALSARQQATELQKLRTEEERRLEEAKSSEEAAMSI 331

Query: 241 AEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRYRKY 300
            E+E+AK+KAA+EAAEAA+R+AE+E+++R+ AEM+ +KE+       D+ S+  +RYRKY
Sbjct: 332 VEKERAKAKAALEAAEAAKRLAEVESKRRLTAEMKTMKES-------DSFSRGFVRYRKY 391

Query: 301 TIEEIEAATEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLS 360
           T++EIE AT  F+ES+K+GEGGYGPV++  LDHT VA+KVLRPDA QGRSQF++EVEVLS
Sbjct: 392 TVDEIEEATSNFAESQKVGEGGYGPVFRGFLDHTSVAVKVLRPDAAQGRSQFQKEVEVLS 451

Query: 361 CIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALL 420
           CIRHPNMVLLLGACPE+G LVYE+MA GSLEDRL  RGNTPP++WQLRF+IAAEI T LL
Sbjct: 452 CIRHPNMVLLLGACPEFGILVYEYMAKGSLEDRLFMRGNTPPITWQLRFRIAAEIATGLL 511

Query: 421 FLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFC 480
           FLHQTKPEP+VHRDLKP N+LLD N+VSKISDVGLARLV P+VA+NVTQYR+TS AGTFC
Sbjct: 512 FLHQTKPEPIVHRDLKPGNVLLDYNYVSKISDVGLARLV-PAVAENVTQYRVTSAAGTFC 571

Query: 481 YIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSD 540
           YIDPEYQQTGMLGVKSD+YSLGIM LQ++T++ PMGL ++VE+AIE+GT   +LDP V D
Sbjct: 572 YIDPEYQQTGMLGVKSDVYSLGIMLLQILTAKQPMGLAYYVEQAIEEGTLKDMLDPAVPD 631

Query: 541 WPVEEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRS 595
           WP+EEALS AK+SL+CAELRRKDRPDLGK ILPELNRLR I E+++      G+   S +
Sbjct: 632 WPIEEALSLAKLSLQCAELRRKDRPDLGKEILPELNRLREIGEESLESVFYAGNQGKSPN 670

BLAST of MS025592 vs. TAIR 10
Match: AT1G78940.2 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 638.6 bits (1646), Expect = 5.0e-183
Identity = 358/603 (59.37%), Postives = 458/603 (75.95%), Query Frame = 0

Query: 1   MRSASRPAPTISPLRGQLAQQCSAKSDATEFHSATGTAARVSIEKPPVGLDFPSKPPQDE 60
           +R+ASRPAP  + +     QQC    +    H  T   A    + P      PS+ P+  
Sbjct: 163 VRNASRPAPYQNSM-----QQC----EIDNHHPHTPDKAPKYHDHPNSAGSTPSR-PRKS 222

Query: 61  LDFMKSPFT-RKGY-NYKASYGDLSM------HDSDISFVSSGRPSIDRIFPSLYDNQDV 120
           ++  +SP   RK Y ++  S  DLS        DSDISF+SSGRPS++R   SL D  + 
Sbjct: 223 VEADRSPLVKRKPYGDFYDSDSDLSFISPSSHRDSDISFISSGRPSVERSSFSL-DFPES 282

Query: 121 GRVTPRMSSSTDLDLTHSFESLHMGRMSLDMSCP---SEFSSISQDSDRL-SISSQAMED 180
            R T RMS+S++       +S+   R+ +  S P   +E S+ S++S R  S SSQ+++D
Sbjct: 283 AR-TSRMSTSSE-------QSIGSNRLGIKFSDPDFLNESSTFSEESGRTSSYSSQSLDD 342

Query: 181 VEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEERRLEEARLAGEAALAL 240
           VEAEM+RL+LELKQTM+MYSTACKEAL+A+Q+A ELQ+ + EEERRLEEA+ + EAA+++
Sbjct: 343 VEAEMKRLRLELKQTMDMYSTACKEALSARQQATELQKLRTEEERRLEEAKSSEEAAMSI 402

Query: 241 AEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMRALKEAEEKKKAVDALSQNDIRYRKY 300
            E+E+AK+KAA+EAAEAA+R+AE+E+++R+ AEM+ +KE+       D+ S+  +RYRKY
Sbjct: 403 VEKERAKAKAALEAAEAAKRLAEVESKRRLTAEMKTMKES-------DSFSRGFVRYRKY 462

Query: 301 TIEEIEAATEFFSESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLS 360
           T++EIE AT  F+ES+K+GEGGYGPV++  LDHT VA+KVLRPDA QGRSQF++EVEVLS
Sbjct: 463 TVDEIEEATSNFAESQKVGEGGYGPVFRGFLDHTSVAVKVLRPDAAQGRSQFQKEVEVLS 522

Query: 361 CIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLARRGNTPPLSWQLRFKIAAEIGTALL 420
           CIRHPNMVLLLGACPE+G LVYE+MA GSLEDRL  RGNTPP++WQLRF+IAAEI T LL
Sbjct: 523 CIRHPNMVLLLGACPEFGILVYEYMAKGSLEDRLFMRGNTPPITWQLRFRIAAEIATGLL 582

Query: 421 FLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFC 480
           FLHQTKPEP+VHRDLKP N+LLD N+VSKISDVGLARLV P+VA+NVTQYR+TS AGTFC
Sbjct: 583 FLHQTKPEPIVHRDLKPGNVLLDYNYVSKISDVGLARLV-PAVAENVTQYRVTSAAGTFC 642

Query: 481 YIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIEKGTFASILDPTVSD 540
           YIDPEYQQTGMLGVKSD+YSLGIM LQ++T++ PMGL ++VE+AIE+GT   +LDP V D
Sbjct: 643 YIDPEYQQTGMLGVKSDVYSLGIMLLQILTAKQPMGLAYYVEQAIEEGTLKDMLDPAVPD 702

Query: 541 WPVEEALSFAKMSLKCAELRRKDRPDLGKAILPELNRLRTIAEDTMHPTLMGGDTSPSRS 592
           WP+EEALS AK+SL+CAELRRKDRPDLGK ILPELNRLR I E+++      G+   S +
Sbjct: 703 WPIEEALSLAKLSLQCAELRRKDRPDLGKEILPELNRLREIGEESLESVFYAGNQGKSPN 738

BLAST of MS025592 vs. TAIR 10
Match: AT5G35380.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 631.7 bits (1628), Expect = 6.1e-181
Identity = 341/529 (64.46%), Postives = 419/529 (79.21%), Query Frame = 0

Query: 83  SMHDSDISFVSSGRPSIDRIFPSLYDNQDVGRVTPRMSSSTDLDLTHSFESLHMGRMSLD 142
           S+ DSDISFVSSGRPS+D++FPSLYD+ DV    PR+S +++        +    + S+D
Sbjct: 210 SITDSDISFVSSGRPSVDQMFPSLYDDVDV----PRLSVTSEYGENRLSFATTYSKQSID 269

Query: 143 MSCP-SEFSSISQDSDRLSISSQAMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 202
           +  P +  SS S +S R S S Q  +++E EMRRLK+ELK TMEMY++ACKEA++AK+ A
Sbjct: 270 LGSPYAPNSSTSFESGRQSFSLQGQDELETEMRRLKMELKHTMEMYNSACKEAISAKKAA 329

Query: 203 VELQRWKLEEERRLEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 262
            EL +WK E+E +LEE RL+ EAA+A+AE+EK KS+AA+EAA AA+++++LEA+KR    
Sbjct: 330 NELLKWKAEKEHKLEEVRLSKEAAMAMAEREKEKSRAAMEAAVAAQKLSDLEAEKR---- 389

Query: 263 MRALKEAEEKKKAVDALSQNDIRYRKYTIEEIEAATEFFSESRKIGEGGYGPVYKCDLDH 322
            + ++  +EKK+AV +L     RYRKYTIEEIE ATE FS SRK+GEGGYGPVYK  LD+
Sbjct: 390 -KHIETVDEKKRAVSSL-----RYRKYTIEEIEEATEDFSPSRKVGEGGYGPVYKGTLDY 449

Query: 323 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 382
           T VAIKVLRPDA QGRSQF++EVEVL+C+RHPNMVLLLGACPEYGCLVYE+MANGSL+D 
Sbjct: 450 TKVAIKVLRPDAAQGRSQFQREVEVLTCMRHPNMVLLLGACPEYGCLVYEYMANGSLDDC 509

Query: 383 LARRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 442
           L RRGN+P LSWQLRF+IA+EI T L FLHQ KPEPLVHRDLKP NILLD++FVSKISDV
Sbjct: 510 LFRRGNSPILSWQLRFRIASEIATGLHFLHQMKPEPLVHRDLKPGNILLDQHFVSKISDV 569

Query: 443 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 502
           GLARLVPPSVAD  TQYRMTSTAGTF YIDPEYQQTGMLG KSDIYS GIM LQ++T++P
Sbjct: 570 GLARLVPPSVADTATQYRMTSTAGTFFYIDPEYQQTGMLGTKSDIYSFGIMLLQILTAKP 629

Query: 503 PMGLTHHVERAIEKGTFASILDPTVSDWPVEEALSFAKMSLKCAELRRKDRPDLGKAILP 562
           PMGLTHHVE+AIEKGTFA +LDP V DWP EEAL+ AK++L+CA+LRRKDRPDLG  +LP
Sbjct: 630 PMGLTHHVEKAIEKGTFAEMLDPAVPDWPFEEALAAAKLALQCAKLRRKDRPDLGNIVLP 689

Query: 563 ELNRLRTIAEDTM-----HPTLM---GGDTSPSRSHSQVSLQLGSMTSS 603
           EL +LR +AE+++      P+ +   G  TS     S   LQ GS +SS
Sbjct: 690 ELKKLRDLAEESIKFGVRQPSPIRSSGSATSIQEIISDPQLQYGSDSSS 724

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144401.10.0e+00100.00U-box domain-containing protein 52-like [Momordica charantia][more]
XP_008466285.13.1e-29688.33PREDICTED: U-box domain-containing protein 52 [Cucumis melo][more]
KAA0038736.12.7e-29286.65U-box domain-containing protein 52 [Cucumis melo var. makuwa][more]
XP_011652539.11.3e-28987.03U-box domain-containing protein 52 [Cucumis sativus] >KGN60182.1 hypothetical pr... [more]
XP_023536596.17.0e-28885.03U-box domain-containing protein 35-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8S8S73.1e-10548.74U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3... [more]
Q9SW111.0e-10346.06U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2... [more]
Q9FKG61.9e-10243.80U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2... [more]
Q9FKG53.6e-10148.85U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2... [more]
Q9LU475.7e-9145.31Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Match NameE-valueIdentityDescription
A0A6J1CTA90.0e+00100.00U-box domain-containing protein 52-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A1S3CQW01.5e-29688.33U-box domain-containing protein 52 OS=Cucumis melo OX=3656 GN=LOC103503739 PE=4 ... [more]
A0A5A7T9561.3e-29286.65U-box domain-containing protein 52 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A0A0LHG86.2e-29087.03Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G882... [more]
A0A6J1FEZ36.4e-28783.65U-box domain-containing protein 52-like isoform X1 OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT2G24370.11.1e-23068.87Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT4G31230.17.6e-21667.23Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT1G78940.12.6e-18459.41Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT1G78940.25.0e-18359.37Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT5G35380.16.1e-18164.46Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 226..278
NoneNo IPR availableCOILSCoilCoilcoord: 192..212
NoneNo IPR availableCOILSCoilCoilcoord: 159..186
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 373..577
e-value: 3.1E-47
score: 162.6
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 277..372
e-value: 1.1E-25
score: 91.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 575..615
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 579..615
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..36
NoneNo IPR availablePANTHERPTHR45647OS02G0152300 PROTEINcoord: 1..611
NoneNo IPR availablePANTHERPTHR45647:SF101U-BOX DOMAIN-CONTAINING PROTEIN 34-LIKEcoord: 1..611
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 300..560
e-value: 8.9E-28
score: 108.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 301..553
e-value: 1.8E-40
score: 139.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 300..578
score: 36.089062
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 418..430
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 282..557

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS025592.1MS025592.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity