MS025478 (gene) Bitter gourd (TR) v1

BLAST of MS025478 vs. NCBI nr
Match: XP_038875327.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 6-like [Benincasa hispida])

HSP 1 Score: 553.5 bits (1425), Expect = 1.4e-153
Identity = 260/371 (70.08%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 12  NGYDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPT-SPIESPAAAMA--AASESD 71
           N YDRQT+LKAF  TKLGVKGLVDSG+T+IP+IF HDD + S I SPAAA+A   A E++
Sbjct: 8   NAYDRQTDLKAFHNTKLGVKGLVDSGVTQIPRIFSHDDQSPSLISSPAAAVAEKLAGETE 67

Query: 72  NSNLGIPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRF 131
             NL IP+IDL+ T +N   R K I++IR A ENWGFFQI+NHG+P  V  EM+NG RRF
Sbjct: 68  AKNLAIPVIDLQQTHKN---RAKIINEIREASENWGFFQILNHGVPLPVMKEMMNGIRRF 127

Query: 132 HEEDDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLACR 191
           HE+D E+K+++YSRDF  RK+LFNTNFDL++GVSTNWRDTL+ VVAP G EPE+IP   R
Sbjct: 128 HEQDGEMKKDIYSRDF-QRKILFNTNFDLFKGVSTNWRDTLTVVVAPHGVEPEEIPAVSR 187

Query: 192 EITAEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGT 251
           E   ++S+RV ELG  L+E  SE LGV PNRL+E+GCG+GMVMF HYYPPCP+PELTWGT
Sbjct: 188 EAILDFSRRVKELGDILLEFLSEGLGVAPNRLKELGCGEGMVMFCHYYPPCPQPELTWGT 247

Query: 252 TDHSDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHR 311
           TDH+D  FLTVLLQD  GGLQVR GDRWVDV P++GA VVN+GDF+QL+SNDRFKSVNHR
Sbjct: 248 TDHTDGCFLTVLLQDDFGGLQVRHGDRWVDVHPIEGAFVVNIGDFMQLMSNDRFKSVNHR 307

Query: 312 VMAKKIGPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQGL 371
           V+A K GPRISVA FFRCNLPPENG V+GP+KEL S++NP IYRET+IKDYVAHYY++GL
Sbjct: 308 VLANKGGPRISVASFFRCNLPPENGLVFGPLKELRSEENPDIYRETSIKDYVAHYYQKGL 367

Query: 372 NGISGLEHFKL 380
           NGIS LEHFKL
Sbjct: 368 NGISALEHFKL 374

BLAST of MS025478 vs. NCBI nr
Match: XP_022138845.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like [Momordica charantia])

HSP 1 Score: 552.7 bits (1423), Expect = 2.4e-153
Identity = 263/388 (67.78%), Postives = 312/388 (80.41%), Query Frame = 0

Query: 1   MMKSCNGETNP--------NGYDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTS 60
           M+ + NGE  P          YDRQ +LKA D +KLGVKGLVD G TK+P+IFIH +  S
Sbjct: 1   MVDNRNGEKIPVEILFSDDGNYDRQRDLKALDGSKLGVKGLVDLGFTKVPRIFIHQNRNS 60

Query: 61  PIESPAAAMAAASESDNSNLGIPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHG 120
               P         SD+SN  IPIIDL++ AQNPQSR   IDQIR ACENWGFFQ++NHG
Sbjct: 61  SDPPP---------SDDSNFTIPIIDLQAAAQNPQSRAAVIDQIRDACENWGFFQVLNHG 120

Query: 121 IPSSVTAEMINGTRRFHEEDDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCV 180
           +PS V AEM++G RRFHE+D E KRNLYSRD+  +KVL+NTNFDL++  +TNWRDT++CV
Sbjct: 121 VPSEVAAEMLDGIRRFHEQDGEQKRNLYSRDY-TKKVLYNTNFDLFQAAATNWRDTITCV 180

Query: 181 VAPRGAEPEDIPLACREITAEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMF 240
           +APRGA PE++PL CR+    YS RV+ELG+ L+EL SE LGVGP+RL+EMGCG+G+V+F
Sbjct: 181 IAPRGARPEEVPLICRDSVLNYSARVMELGRNLLELVSEGLGVGPDRLKEMGCGEGLVLF 240

Query: 241 SHYYPPCPEPELTWGTTDHSDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGD 300
            HYYP CPEPELT GT+DHSDSSFLTVLLQDQIGGLQVRRG+RWVDV PVDGALVVN+GD
Sbjct: 241 GHYYPACPEPELTLGTSDHSDSSFLTVLLQDQIGGLQVRRGERWVDVPPVDGALVVNLGD 300

Query: 301 FLQLISNDRFKSVNHRVMAKKIGPRISVACFFRCNLPPENG-RVYGPIKELISDQNPAIY 360
            LQLISN RFKSV HRV+AKK GPRISVACFFR N PPE+  +VYGPIKELIS++NP +Y
Sbjct: 301 MLQLISNGRFKSVKHRVIAKKEGPRISVACFFRYNCPPESELKVYGPIKELISEENPPLY 360

Query: 361 RETTIKDYVAHYYEQGLNGISGLEHFKL 380
           RETTIK++VAHYY +GLNG+S LEHFKL
Sbjct: 361 RETTIKNFVAHYYSKGLNGVSALEHFKL 378

BLAST of MS025478 vs. NCBI nr
Match: XP_008460628.1 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase homolog 6 [Cucumis melo] >KAA0065679.1 1-aminocyclopropane-1-carboxylate oxidase-like protein 6 [Cucumis melo var. makuwa] >TYK08223.1 1-aminocyclopropane-1-carboxylate oxidase-like protein 6 [Cucumis melo var. makuwa])

HSP 1 Score: 541.6 bits (1394), Expect = 5.5e-150
Identity = 253/372 (68.01%), Postives = 309/372 (83.06%), Query Frame = 0

Query: 12  NGYDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPT--SPIESPAAAMA--AASES 71
           N YDRQ++L+AF  TKLGVKGL D+G++++P+IF HD+ +  S I SPAAA A   A E+
Sbjct: 9   NSYDRQSDLEAFHSTKLGVKGLADAGVSQLPRIFCHDNQSAASLISSPAAAAAEKLAGET 68

Query: 72  DNSNLGIPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRR 131
           +  NL IP+IDL+ + +N   R + I++I+ AC+NWGFFQI+NHG+P SV  EM+NG RR
Sbjct: 69  EAKNLTIPVIDLQDSHKN---RAQIINEIKDACKNWGFFQILNHGVPLSVMKEMMNGIRR 128

Query: 132 FHEEDDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLAC 191
           FHE+ DE+K++LYSRDF  RK+LFNTNFDL++GVSTNWRDTL+ VVAPRGAE E+IP   
Sbjct: 129 FHEQGDEMKKDLYSRDF-QRKILFNTNFDLFKGVSTNWRDTLTVVVAPRGAEAEEIPEVS 188

Query: 192 REITAEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWG 251
           RE   E+S+RV ELG  L+E   E LGVG NRL+EMGCGDGMVMF HYYP CP+PELTWG
Sbjct: 189 REAVVEFSRRVKELGDILLEFLGEGLGVGSNRLKEMGCGDGMVMFCHYYPACPQPELTWG 248

Query: 252 TTDHSDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNH 311
           TTDH+DSSFLTVLLQD++GGLQVR GDRWVDV P++GA VVN+GDF+Q++SNDRF SVNH
Sbjct: 249 TTDHTDSSFLTVLLQDELGGLQVRHGDRWVDVHPIEGAFVVNIGDFMQMVSNDRFISVNH 308

Query: 312 RVMAKKIGPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQG 371
           RV+A K GPRISVA FFRCNLPPENG V+GP+KEL S++NP IY+ET+IKDYVAHYY +G
Sbjct: 309 RVLANKKGPRISVASFFRCNLPPENGLVFGPLKELRSEENPDIYKETSIKDYVAHYYNKG 368

Query: 372 LNGISGLEHFKL 380
           LNGIS LEHFKL
Sbjct: 369 LNGISALEHFKL 376

BLAST of MS025478 vs. NCBI nr
Match: XP_023513998.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 537.7 bits (1384), Expect = 8.0e-149
Identity = 258/369 (69.92%), Postives = 298/369 (80.76%), Query Frame = 0

Query: 12  NGYDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSN 71
           + YDRQ ELKA DE+KLGVKGLVDSGLT +PKIF+H +  S       +  AA  SD+S 
Sbjct: 18  HAYDRQRELKALDESKLGVKGLVDSGLTNLPKIFLHQNQKS-------SSTAALPSDDSR 77

Query: 72  LGIPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEE 131
             IPIIDL STAQNPQSR   IDQIR A ENWGFFQI+NHGIPS+VTAEM  G RRFHE+
Sbjct: 78  FTIPIIDLHSTAQNPQSRSIIIDQIRDASENWGFFQILNHGIPSTVTAEMQAGIRRFHEQ 137

Query: 132 DDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLACREIT 191
           DDE K+  YSRD+  + VL+NTNFDLY+   TNWRDTL+C +APRGAEP+DIPL CR+I 
Sbjct: 138 DDEQKKQFYSRDY-TKTVLYNTNFDLYQAPVTNWRDTLTCAIAPRGAEPKDIPLICRDII 197

Query: 192 AEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDH 251
             Y+KRVLELG+ L EL SEALG+G NRL E+GC +G+V+F HYYP CPEPEL  GT+DH
Sbjct: 198 LNYTKRVLELGKTLFELVSEALGLGSNRLNEIGCSEGLVLFGHYYPACPEPELAIGTSDH 257

Query: 252 SDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMA 311
           SDSSFLTVLLQDQIGGLQVR  DRWVDV P+DGALV+N+GD LQL+SN R KS+NHRVMA
Sbjct: 258 SDSSFLTVLLQDQIGGLQVRHDDRWVDVRPIDGALVINIGDMLQLVSNGRLKSLNHRVMA 317

Query: 312 KKIGPRISVACFFRCNLPPENGR-VYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNG 371
           K  GPRISVACFFR   PPE+ R VYGPIKELIS++ PA+YRETTIK++VAHYY +GLNG
Sbjct: 318 KSEGPRISVACFFRYQFPPESERKVYGPIKELISEEKPAVYRETTIKNFVAHYYTKGLNG 377

Query: 372 ISGLEHFKL 380
           +S LE FKL
Sbjct: 378 VSALEQFKL 378

BLAST of MS025478 vs. NCBI nr
Match: XP_023000510.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 3-like [Cucurbita maxima] >XP_023000511.1 1-aminocyclopropane-1-carboxylate oxidase homolog 3-like [Cucurbita maxima])

HSP 1 Score: 531.6 bits (1368), Expect = 5.7e-147
Identity = 254/369 (68.83%), Postives = 296/369 (80.22%), Query Frame = 0

Query: 12  NGYDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSN 71
           + YDRQ ELKA DE+KLGVKGLVDSGLT +PKIF+H +  S       +  AA  SD+S 
Sbjct: 18  HAYDRQRELKALDESKLGVKGLVDSGLTNLPKIFLHQNQKS-------SSTAALPSDDSR 77

Query: 72  LGIPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEE 131
             IPIIDL STAQNPQ R   IDQIR A ENWGFFQI+NHG+PS+VTAEM+ G RRFHE+
Sbjct: 78  FTIPIIDLHSTAQNPQLRSIIIDQIRDASENWGFFQILNHGVPSTVTAEMLAGIRRFHEQ 137

Query: 132 DDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLACREIT 191
           D E K   YSRD+  + VL+NTNFDLY+   TNWRDTL+C +APRGAE +D+PL CR+I 
Sbjct: 138 DGEQKTQFYSRDY-TKTVLYNTNFDLYKAPVTNWRDTLTCAIAPRGAESKDMPLICRDII 197

Query: 192 AEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDH 251
             Y+KRVLELG+ L EL SEALG+GPNRL E+GCG+G+V+F HYYP CPEPEL  GT+DH
Sbjct: 198 LNYTKRVLELGKTLFELVSEALGLGPNRLNEIGCGEGLVLFGHYYPACPEPELAIGTSDH 257

Query: 252 SDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMA 311
           SDSSFLTVLLQDQIGGLQVR  DRWVDV P+DGALV+N+GD LQL+SN R KSVNHRV+A
Sbjct: 258 SDSSFLTVLLQDQIGGLQVRHDDRWVDVRPIDGALVINIGDMLQLVSNGRLKSVNHRVLA 317

Query: 312 KKIGPRISVACFFRCNLPPENGR-VYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNG 371
           K  G RISVACFFR   PPE+ R VYGPIKELIS++ PA+YRETTIK++VAHYY +GLNG
Sbjct: 318 KSEGARISVACFFRYQFPPESERKVYGPIKELISEEKPAVYRETTIKNFVAHYYTKGLNG 377

Query: 372 ISGLEHFKL 380
           +S LE FKL
Sbjct: 378 VSALEQFKL 378

BLAST of MS025478 vs. ExPASy Swiss-Prot
Match: Q84MB3 (1-aminocyclopropane-1-carboxylate oxidase homolog 1 OS=Arabidopsis thaliana OX=3702 GN=At1g06620 PE=2 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 1.6e-112
Identity = 194/365 (53.15%), Postives = 251/365 (68.77%), Query Frame = 0

Query: 15  DRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSNLGI 74
           DR T LKAFDETK GVKGL+D+G+T+IP IF          +P A + +     +S+  I
Sbjct: 12  DRSTLLKAFDETKTGVKGLIDAGITEIPSIF---------RAPPATLTSPKPPSSSDFSI 71

Query: 75  PIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEEDDE 134
           P IDL+    +  +R   +++I  A E WGFFQ+INHGIP  V  +MI+G R FHE+D E
Sbjct: 72  PTIDLKGGGTDSITRRSLVEKIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTE 131

Query: 135 IKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLACREITAEY 194
           +K+  YSRD  A K+++++NFDL+   + NWRDTL C  AP    PED+P  C E+  EY
Sbjct: 132 VKKGFYSRD-PASKMVYSSNFDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEY 191

Query: 195 SKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDHSDS 254
           SK V++LG+ L EL SEALG+  N L++M C + +++  HYYPPCP+P+LT G T HSD+
Sbjct: 192 SKEVMKLGKLLFELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDN 251

Query: 255 SFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMAKKI 314
           SFLT+LLQD IGGLQV     WVDV PV GALVVNVGD LQLI+ND+F SV HRV+A   
Sbjct: 252 SFLTILLQDHIGGLQVLHDQYWVDVPPVPGALVVNVGDLLQLITNDKFISVEHRVLANVA 311

Query: 315 GPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNGISGL 374
           GPRISVACFF   L   N RVYGPIKE++S++NP  YR+TTI +Y   Y  +G +G SGL
Sbjct: 312 GPRISVACFFSSYL-MANPRVYGPIKEILSEENPPNYRDTTITEYAKFYRSKGFDGTSGL 365

Query: 375 EHFKL 380
            + K+
Sbjct: 372 LYLKI 365

BLAST of MS025478 vs. ExPASy Swiss-Prot
Match: Q9LTH7 (1-aminocyclopropane-1-carboxylate oxidase homolog 12 OS=Arabidopsis thaliana OX=3702 GN=At5g59540 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 9.0e-111
Identity = 193/367 (52.59%), Postives = 255/367 (69.48%), Query Frame = 0

Query: 14  YDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSNLG 73
           +D   E KAFDETK GVKGLVD+ +T++P+IF H       + P+A++        S+L 
Sbjct: 9   FDPYIERKAFDETKQGVKGLVDAKITEVPRIFHHRQDILTNKKPSASV--------SDLE 68

Query: 74  IPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEEDD 133
           IPIID  S   +  SR   +++++ A ENWGFFQ+INH IP +V  E+ +G RRFHEED 
Sbjct: 69  IPIIDFASVHADTASREAIVEKVKYAVENWGFFQVINHSIPLNVLEEIKDGVRRFHEEDP 128

Query: 134 EIKRNLYSRDFVARKVLFNTNFDLY-RGVSTNWRDTLSCVVAPRGAEPEDIPLACREITA 193
           E+K++ +SRD   +K ++N+NFDLY    S NWRD+ SC +AP    PE+IP  CR+   
Sbjct: 129 EVKKSFFSRDAGNKKFVYNSNFDLYSSSPSVNWRDSFSCYIAPDPPAPEEIPETCRDAMF 188

Query: 194 EYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDHS 253
           EYSK VL  G  L EL SEALG+    L  M C   ++M  HYYPPCP+P+LT G T HS
Sbjct: 189 EYSKHVLSFGGLLFELLSEALGLKSQTLESMDCVKTLLMICHYYPPCPQPDLTLGITKHS 248

Query: 254 DSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMAK 313
           D+SFLT+LLQD IGGLQ+   D WVDVSP+ GALVVN+GDFLQLI+ND+F SV HRV+A 
Sbjct: 249 DNSFLTLLLQDNIGGLQILHQDSWVDVSPIHGALVVNIGDFLQLITNDKFVSVEHRVLAN 308

Query: 314 KIGPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNGIS 373
           + GPRISVA FF  ++ P N RVYGP+KEL+S++NP  YR+ TIK+Y   ++E+GL+G S
Sbjct: 309 RQGPRISVASFFSSSMRP-NSRVYGPMKELVSEENPPKYRDITIKEYSKIFFEKGLDGTS 366

Query: 374 GLEHFKL 380
            L + ++
Sbjct: 369 HLSNIRI 366

BLAST of MS025478 vs. ExPASy Swiss-Prot
Match: Q8H1S4 (1-aminocyclopropane-1-carboxylate oxidase homolog 3 OS=Arabidopsis thaliana OX=3702 GN=At1g06650 PE=2 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 2.0e-110
Identity = 196/369 (53.12%), Postives = 259/369 (70.19%), Query Frame = 0

Query: 14  YDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSNL- 73
           +DR +ELKAFDETK GVKGLVDSG++++P+IF H  PT  + +P         SD  +L 
Sbjct: 10  FDRASELKAFDETKTGVKGLVDSGVSQVPRIFHH--PTVKLSTPKPL-----PSDLLHLK 69

Query: 74  GIPIIDLES-TAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEE 133
            IP IDL     Q+   R   I++I+ A   WGFFQ+INHG+   +  +M  G R FHE+
Sbjct: 70  TIPTIDLGGRDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQ 129

Query: 134 DDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLACREIT 193
             E+++  YSRDF +R+ L+ +NFDL+   + NWRDT SC +AP   +P+D+P  CR+I 
Sbjct: 130 SQEVRKEFYSRDF-SRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIM 189

Query: 194 AEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDH 253
            EYSK+V+ LG+FL EL SEALG+ PN L +M C  G++M SHYYPPCPEP+LT GT+ H
Sbjct: 190 MEYSKQVMNLGKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQH 249

Query: 254 SDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMA 313
           SD+SFLTVLL DQI GLQVRR   W DV  V GAL++N+GD LQLI+ND+F S+ HRV+A
Sbjct: 250 SDNSFLTVLLPDQIEGLQVRREGHWFDVPHVSGALIINIGDLLQLITNDKFISLEHRVLA 309

Query: 314 KK-IGPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNG 373
            +    R+SVACFF   + P N R+YGPI+EL+S++NP  YRETTIKDY  ++  +GL+G
Sbjct: 310 NRATRARVSVACFFTTGVRP-NPRMYGPIRELVSEENPPKYRETTIKDYATYFNAKGLDG 369

Query: 374 ISGLEHFKL 380
            S L HFK+
Sbjct: 370 TSALLHFKI 369

BLAST of MS025478 vs. ExPASy Swiss-Prot
Match: Q9LTH8 (1-aminocyclopropane-1-carboxylate oxidase homolog 11 OS=Arabidopsis thaliana OX=3702 GN=At5g59530 PE=2 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 2.7e-107
Identity = 190/368 (51.63%), Postives = 253/368 (68.75%), Query Frame = 0

Query: 14  YDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSNLG 73
           +DR  E KAFD TK GVKGL+D+ +T+IP+IF     T P +  +           S+L 
Sbjct: 8   FDRYIERKAFDNTKEGVKGLIDAKITEIPRIFHVPQDTLPDKKRSV----------SDLE 67

Query: 74  IPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEEDD 133
           IP ID  S   +  SR   +++++ A ENWGFFQ+INHG+P +V  E+ +G RRFHEE+D
Sbjct: 68  IPTIDFASVNVDTPSREAIVEKVKYAVENWGFFQVINHGVPLNVLEEIKDGVRRFHEEED 127

Query: 134 -EIKRNLYSRDFVARKVLFNTNFDLY-RGVSTNWRDTLSCVVAPRGAEPEDIPLACREIT 193
            E+K++ YS DF   K  +++NFDLY    S  WRD++SC +AP    PE++P  CR+  
Sbjct: 128 PEVKKSYYSLDFTKNKFAYSSNFDLYSSSPSLTWRDSISCYMAPDPPTPEELPETCRDAM 187

Query: 194 AEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDH 253
            EYSK VL LG  L EL SEALG+    L+ M C   ++M  HYYPPCP+P+LT G + H
Sbjct: 188 IEYSKHVLSLGDLLFELLSEALGLKSEILKSMDCLKSLLMICHYYPPCPQPDLTLGISKH 247

Query: 254 SDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMA 313
           SD+SFLTVLLQD IGGLQ+   D WVDVSP+ GALVVNVGDFLQLI+ND+F SV HRV+A
Sbjct: 248 SDNSFLTVLLQDNIGGLQILHQDSWVDVSPLPGALVVNVGDFLQLITNDKFISVEHRVLA 307

Query: 314 KKIGPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNGI 373
              GPRISVA FF  ++  EN  VYGP+KEL+S++NP  YR+TT+++Y   Y+++GL+G 
Sbjct: 308 NTRGPRISVASFFSSSI-RENSTVYGPMKELVSEENPPKYRDTTLREYSEGYFKKGLDGT 364

Query: 374 SGLEHFKL 380
           S L +F++
Sbjct: 368 SHLSNFRI 364

BLAST of MS025478 vs. ExPASy Swiss-Prot
Match: O04847 (Deacetoxyvindoline 4-hydroxylase OS=Catharanthus roseus OX=4058 GN=D4H PE=1 SV=2)

HSP 1 Score: 385.6 bits (989), Expect = 6.7e-106
Identity = 178/364 (48.90%), Postives = 250/364 (68.68%), Query Frame = 0

Query: 19  ELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSNLGIPIID 78
           ELKAFDETK GVKG+VD+G+TKIP+IF        I+ P      +     S++ IP+I+
Sbjct: 44  ELKAFDETKAGVKGIVDTGITKIPRIF--------IDQPKNLDRISVCRGKSDIKIPVIN 103

Query: 79  LESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEEDDEIKRN 138
           L   + N + R + +++I  A E +GFFQI+NHGIP  V  +M++G R+FHE+DD+IKR 
Sbjct: 104 LNGLSSNSEIRREIVEKIGEASEKYGFFQIVNHGIPQDVMDKMVDGVRKFHEQDDQIKRQ 163

Query: 139 LYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLACREITAEYSKRV 198
            YSRD   +  L+++N+ L  G++ NWRDT+ C++     +P++ P  CR+I  +YS  V
Sbjct: 164 YYSRDRFNKNFLYSSNYVLIPGIACNWRDTMECIMNSNQPDPQEFPDVCRDILMKYSNYV 223

Query: 199 LELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDHSDSSFLT 258
             LG  L EL SEALG+ PN L EM C +G+++  HYYP CP+PELT+GT+ HSDS FLT
Sbjct: 224 RNLGLILFELLSEALGLKPNHLEEMDCAEGLILLGHYYPACPQPELTFGTSKHSDSGFLT 283

Query: 259 VLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMAKKIGPRI 318
           +L+QDQIGGLQ+   ++W+DV  + GALV+N+ D LQLI+ND+FKSV HRV+A K+GPRI
Sbjct: 284 ILMQDQIGGLQILLENQWIDVPFIPGALVINIADLLQLITNDKFKSVEHRVLANKVGPRI 343

Query: 319 SVACFFRCNLPPENG---RVYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNGISGLE 378
           SVA  F      + G   R+YGPIKELIS++NP IY+E T+KD++   + +  +  S L 
Sbjct: 344 SVAVAFGIKTQTQEGVSPRLYGPIKELISEENPPIYKEVTVKDFITIRFAKRFDDSSSLS 399

Query: 379 HFKL 380
            F+L
Sbjct: 404 PFRL 399

BLAST of MS025478 vs. ExPASy TrEMBL
Match: A0A6J1CAN0 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like OS=Momordica charantia OX=3673 GN=LOC111009916 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 1.2e-153
Identity = 263/388 (67.78%), Postives = 312/388 (80.41%), Query Frame = 0

Query: 1   MMKSCNGETNP--------NGYDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTS 60
           M+ + NGE  P          YDRQ +LKA D +KLGVKGLVD G TK+P+IFIH +  S
Sbjct: 1   MVDNRNGEKIPVEILFSDDGNYDRQRDLKALDGSKLGVKGLVDLGFTKVPRIFIHQNRNS 60

Query: 61  PIESPAAAMAAASESDNSNLGIPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHG 120
               P         SD+SN  IPIIDL++ AQNPQSR   IDQIR ACENWGFFQ++NHG
Sbjct: 61  SDPPP---------SDDSNFTIPIIDLQAAAQNPQSRAAVIDQIRDACENWGFFQVLNHG 120

Query: 121 IPSSVTAEMINGTRRFHEEDDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCV 180
           +PS V AEM++G RRFHE+D E KRNLYSRD+  +KVL+NTNFDL++  +TNWRDT++CV
Sbjct: 121 VPSEVAAEMLDGIRRFHEQDGEQKRNLYSRDY-TKKVLYNTNFDLFQAAATNWRDTITCV 180

Query: 181 VAPRGAEPEDIPLACREITAEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMF 240
           +APRGA PE++PL CR+    YS RV+ELG+ L+EL SE LGVGP+RL+EMGCG+G+V+F
Sbjct: 181 IAPRGARPEEVPLICRDSVLNYSARVMELGRNLLELVSEGLGVGPDRLKEMGCGEGLVLF 240

Query: 241 SHYYPPCPEPELTWGTTDHSDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGD 300
            HYYP CPEPELT GT+DHSDSSFLTVLLQDQIGGLQVRRG+RWVDV PVDGALVVN+GD
Sbjct: 241 GHYYPACPEPELTLGTSDHSDSSFLTVLLQDQIGGLQVRRGERWVDVPPVDGALVVNLGD 300

Query: 301 FLQLISNDRFKSVNHRVMAKKIGPRISVACFFRCNLPPENG-RVYGPIKELISDQNPAIY 360
            LQLISN RFKSV HRV+AKK GPRISVACFFR N PPE+  +VYGPIKELIS++NP +Y
Sbjct: 301 MLQLISNGRFKSVKHRVIAKKEGPRISVACFFRYNCPPESELKVYGPIKELISEENPPLY 360

Query: 361 RETTIKDYVAHYYEQGLNGISGLEHFKL 380
           RETTIK++VAHYY +GLNG+S LEHFKL
Sbjct: 361 RETTIKNFVAHYYSKGLNGVSALEHFKL 378

BLAST of MS025478 vs. ExPASy TrEMBL
Match: A0A5A7VJN4 (1-aminocyclopropane-1-carboxylate oxidase-like protein 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00470 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.7e-150
Identity = 253/372 (68.01%), Postives = 309/372 (83.06%), Query Frame = 0

Query: 12  NGYDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPT--SPIESPAAAMA--AASES 71
           N YDRQ++L+AF  TKLGVKGL D+G++++P+IF HD+ +  S I SPAAA A   A E+
Sbjct: 9   NSYDRQSDLEAFHSTKLGVKGLADAGVSQLPRIFCHDNQSAASLISSPAAAAAEKLAGET 68

Query: 72  DNSNLGIPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRR 131
           +  NL IP+IDL+ + +N   R + I++I+ AC+NWGFFQI+NHG+P SV  EM+NG RR
Sbjct: 69  EAKNLTIPVIDLQDSHKN---RAQIINEIKDACKNWGFFQILNHGVPLSVMKEMMNGIRR 128

Query: 132 FHEEDDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLAC 191
           FHE+ DE+K++LYSRDF  RK+LFNTNFDL++GVSTNWRDTL+ VVAPRGAE E+IP   
Sbjct: 129 FHEQGDEMKKDLYSRDF-QRKILFNTNFDLFKGVSTNWRDTLTVVVAPRGAEAEEIPEVS 188

Query: 192 REITAEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWG 251
           RE   E+S+RV ELG  L+E   E LGVG NRL+EMGCGDGMVMF HYYP CP+PELTWG
Sbjct: 189 REAVVEFSRRVKELGDILLEFLGEGLGVGSNRLKEMGCGDGMVMFCHYYPACPQPELTWG 248

Query: 252 TTDHSDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNH 311
           TTDH+DSSFLTVLLQD++GGLQVR GDRWVDV P++GA VVN+GDF+Q++SNDRF SVNH
Sbjct: 249 TTDHTDSSFLTVLLQDELGGLQVRHGDRWVDVHPIEGAFVVNIGDFMQMVSNDRFISVNH 308

Query: 312 RVMAKKIGPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQG 371
           RV+A K GPRISVA FFRCNLPPENG V+GP+KEL S++NP IY+ET+IKDYVAHYY +G
Sbjct: 309 RVLANKKGPRISVASFFRCNLPPENGLVFGPLKELRSEENPDIYKETSIKDYVAHYYNKG 368

Query: 372 LNGISGLEHFKL 380
           LNGIS LEHFKL
Sbjct: 369 LNGISALEHFKL 376

BLAST of MS025478 vs. ExPASy TrEMBL
Match: A0A1S3CCZ4 (1-aminocyclopropane-1-carboxylate oxidase homolog 6 OS=Cucumis melo OX=3656 GN=LOC103499407 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.7e-150
Identity = 253/372 (68.01%), Postives = 309/372 (83.06%), Query Frame = 0

Query: 12  NGYDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPT--SPIESPAAAMA--AASES 71
           N YDRQ++L+AF  TKLGVKGL D+G++++P+IF HD+ +  S I SPAAA A   A E+
Sbjct: 9   NSYDRQSDLEAFHSTKLGVKGLADAGVSQLPRIFCHDNQSAASLISSPAAAAAEKLAGET 68

Query: 72  DNSNLGIPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRR 131
           +  NL IP+IDL+ + +N   R + I++I+ AC+NWGFFQI+NHG+P SV  EM+NG RR
Sbjct: 69  EAKNLTIPVIDLQDSHKN---RAQIINEIKDACKNWGFFQILNHGVPLSVMKEMMNGIRR 128

Query: 132 FHEEDDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLAC 191
           FHE+ DE+K++LYSRDF  RK+LFNTNFDL++GVSTNWRDTL+ VVAPRGAE E+IP   
Sbjct: 129 FHEQGDEMKKDLYSRDF-QRKILFNTNFDLFKGVSTNWRDTLTVVVAPRGAEAEEIPEVS 188

Query: 192 REITAEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWG 251
           RE   E+S+RV ELG  L+E   E LGVG NRL+EMGCGDGMVMF HYYP CP+PELTWG
Sbjct: 189 REAVVEFSRRVKELGDILLEFLGEGLGVGSNRLKEMGCGDGMVMFCHYYPACPQPELTWG 248

Query: 252 TTDHSDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNH 311
           TTDH+DSSFLTVLLQD++GGLQVR GDRWVDV P++GA VVN+GDF+Q++SNDRF SVNH
Sbjct: 249 TTDHTDSSFLTVLLQDELGGLQVRHGDRWVDVHPIEGAFVVNIGDFMQMVSNDRFISVNH 308

Query: 312 RVMAKKIGPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQG 371
           RV+A K GPRISVA FFRCNLPPENG V+GP+KEL S++NP IY+ET+IKDYVAHYY +G
Sbjct: 309 RVLANKKGPRISVASFFRCNLPPENGLVFGPLKELRSEENPDIYKETSIKDYVAHYYNKG 368

Query: 372 LNGISGLEHFKL 380
           LNGIS LEHFKL
Sbjct: 369 LNGISALEHFKL 376

BLAST of MS025478 vs. ExPASy TrEMBL
Match: A0A6J1KIJ8 (1-aminocyclopropane-1-carboxylate oxidase homolog 3-like OS=Cucurbita maxima OX=3661 GN=LOC111494760 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.8e-147
Identity = 254/369 (68.83%), Postives = 296/369 (80.22%), Query Frame = 0

Query: 12  NGYDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSN 71
           + YDRQ ELKA DE+KLGVKGLVDSGLT +PKIF+H +  S       +  AA  SD+S 
Sbjct: 18  HAYDRQRELKALDESKLGVKGLVDSGLTNLPKIFLHQNQKS-------SSTAALPSDDSR 77

Query: 72  LGIPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEE 131
             IPIIDL STAQNPQ R   IDQIR A ENWGFFQI+NHG+PS+VTAEM+ G RRFHE+
Sbjct: 78  FTIPIIDLHSTAQNPQLRSIIIDQIRDASENWGFFQILNHGVPSTVTAEMLAGIRRFHEQ 137

Query: 132 DDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLACREIT 191
           D E K   YSRD+  + VL+NTNFDLY+   TNWRDTL+C +APRGAE +D+PL CR+I 
Sbjct: 138 DGEQKTQFYSRDY-TKTVLYNTNFDLYKAPVTNWRDTLTCAIAPRGAESKDMPLICRDII 197

Query: 192 AEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDH 251
             Y+KRVLELG+ L EL SEALG+GPNRL E+GCG+G+V+F HYYP CPEPEL  GT+DH
Sbjct: 198 LNYTKRVLELGKTLFELVSEALGLGPNRLNEIGCGEGLVLFGHYYPACPEPELAIGTSDH 257

Query: 252 SDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMA 311
           SDSSFLTVLLQDQIGGLQVR  DRWVDV P+DGALV+N+GD LQL+SN R KSVNHRV+A
Sbjct: 258 SDSSFLTVLLQDQIGGLQVRHDDRWVDVRPIDGALVINIGDMLQLVSNGRLKSVNHRVLA 317

Query: 312 KKIGPRISVACFFRCNLPPENGR-VYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNG 371
           K  G RISVACFFR   PPE+ R VYGPIKELIS++ PA+YRETTIK++VAHYY +GLNG
Sbjct: 318 KSEGARISVACFFRYQFPPESERKVYGPIKELISEEKPAVYRETTIKNFVAHYYTKGLNG 377

Query: 372 ISGLEHFKL 380
           +S LE FKL
Sbjct: 378 VSALEQFKL 378

BLAST of MS025478 vs. ExPASy TrEMBL
Match: A0A6J1ERB7 (1-aminocyclopropane-1-carboxylate oxidase homolog 3-like OS=Cucurbita moschata OX=3662 GN=LOC111436861 PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 6.2e-147
Identity = 253/369 (68.56%), Postives = 297/369 (80.49%), Query Frame = 0

Query: 12  NGYDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSN 71
           + YDRQ ELKA DE+KLGVKGLVDSGLT +PKIF+H +  S       +  AA  SD+S 
Sbjct: 18  HAYDRQRELKALDESKLGVKGLVDSGLTNLPKIFLHQNQKS-------SSTAALPSDDSR 77

Query: 72  LGIPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEE 131
             IPIIDL STAQNPQ R   IDQIR A ENWGFFQI+NHGIPS+VTAE++ G RRFHE+
Sbjct: 78  FTIPIIDLHSTAQNPQLRSIIIDQIRDASENWGFFQILNHGIPSTVTAEILAGIRRFHEQ 137

Query: 132 DDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLACREIT 191
           + E K+  YSRD+  + VL+NTNFDLY+   TNWRDTL+C +APRGAEP+DIPL CR+I 
Sbjct: 138 EGEQKKQFYSRDY-TKTVLYNTNFDLYQAPVTNWRDTLTCAIAPRGAEPKDIPLICRDII 197

Query: 192 AEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDH 251
             Y+KRVLELG+ L EL SEALG+ PNRL E+GC +G+V+F HYYP CPEPEL  GT+DH
Sbjct: 198 LNYTKRVLELGKTLFELVSEALGLDPNRLNEIGCSEGLVLFGHYYPACPEPELAIGTSDH 257

Query: 252 SDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMA 311
           SDSSFLTVLLQDQIGGLQVR  DRWVDV P+DGALV+N+GD LQL+SN R KS+NHRV+A
Sbjct: 258 SDSSFLTVLLQDQIGGLQVRHDDRWVDVRPIDGALVINIGDMLQLVSNGRLKSLNHRVVA 317

Query: 312 KKIGPRISVACFFRCNLPPENGR-VYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNG 371
           K  GPRISVACFFR   PPE+ R VYGPIKELIS++ PA+YRETTIK++VAHYY +GLNG
Sbjct: 318 KSEGPRISVACFFRYQFPPESERKVYGPIKELISEEKPAVYRETTIKNFVAHYYTKGLNG 377

Query: 372 ISGLEHFKL 380
           +S LE FKL
Sbjct: 378 VSALEQFKL 378

BLAST of MS025478 vs. TAIR 10
Match: AT1G06620.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 407.5 bits (1046), Expect = 1.2e-113
Identity = 194/365 (53.15%), Postives = 251/365 (68.77%), Query Frame = 0

Query: 15  DRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSNLGI 74
           DR T LKAFDETK GVKGL+D+G+T+IP IF          +P A + +     +S+  I
Sbjct: 12  DRSTLLKAFDETKTGVKGLIDAGITEIPSIF---------RAPPATLTSPKPPSSSDFSI 71

Query: 75  PIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEEDDE 134
           P IDL+    +  +R   +++I  A E WGFFQ+INHGIP  V  +MI+G R FHE+D E
Sbjct: 72  PTIDLKGGGTDSITRRSLVEKIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTE 131

Query: 135 IKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLACREITAEY 194
           +K+  YSRD  A K+++++NFDL+   + NWRDTL C  AP    PED+P  C E+  EY
Sbjct: 132 VKKGFYSRD-PASKMVYSSNFDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEY 191

Query: 195 SKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDHSDS 254
           SK V++LG+ L EL SEALG+  N L++M C + +++  HYYPPCP+P+LT G T HSD+
Sbjct: 192 SKEVMKLGKLLFELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDN 251

Query: 255 SFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMAKKI 314
           SFLT+LLQD IGGLQV     WVDV PV GALVVNVGD LQLI+ND+F SV HRV+A   
Sbjct: 252 SFLTILLQDHIGGLQVLHDQYWVDVPPVPGALVVNVGDLLQLITNDKFISVEHRVLANVA 311

Query: 315 GPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNGISGL 374
           GPRISVACFF   L   N RVYGPIKE++S++NP  YR+TTI +Y   Y  +G +G SGL
Sbjct: 312 GPRISVACFFSSYL-MANPRVYGPIKEILSEENPPNYRDTTITEYAKFYRSKGFDGTSGL 365

Query: 375 EHFKL 380
            + K+
Sbjct: 372 LYLKI 365

BLAST of MS025478 vs. TAIR 10
Match: AT5G59540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 401.7 bits (1031), Expect = 6.4e-112
Identity = 193/367 (52.59%), Postives = 255/367 (69.48%), Query Frame = 0

Query: 14  YDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSNLG 73
           +D   E KAFDETK GVKGLVD+ +T++P+IF H       + P+A++        S+L 
Sbjct: 9   FDPYIERKAFDETKQGVKGLVDAKITEVPRIFHHRQDILTNKKPSASV--------SDLE 68

Query: 74  IPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEEDD 133
           IPIID  S   +  SR   +++++ A ENWGFFQ+INH IP +V  E+ +G RRFHEED 
Sbjct: 69  IPIIDFASVHADTASREAIVEKVKYAVENWGFFQVINHSIPLNVLEEIKDGVRRFHEEDP 128

Query: 134 EIKRNLYSRDFVARKVLFNTNFDLY-RGVSTNWRDTLSCVVAPRGAEPEDIPLACREITA 193
           E+K++ +SRD   +K ++N+NFDLY    S NWRD+ SC +AP    PE+IP  CR+   
Sbjct: 129 EVKKSFFSRDAGNKKFVYNSNFDLYSSSPSVNWRDSFSCYIAPDPPAPEEIPETCRDAMF 188

Query: 194 EYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDHS 253
           EYSK VL  G  L EL SEALG+    L  M C   ++M  HYYPPCP+P+LT G T HS
Sbjct: 189 EYSKHVLSFGGLLFELLSEALGLKSQTLESMDCVKTLLMICHYYPPCPQPDLTLGITKHS 248

Query: 254 DSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMAK 313
           D+SFLT+LLQD IGGLQ+   D WVDVSP+ GALVVN+GDFLQLI+ND+F SV HRV+A 
Sbjct: 249 DNSFLTLLLQDNIGGLQILHQDSWVDVSPIHGALVVNIGDFLQLITNDKFVSVEHRVLAN 308

Query: 314 KIGPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNGIS 373
           + GPRISVA FF  ++ P N RVYGP+KEL+S++NP  YR+ TIK+Y   ++E+GL+G S
Sbjct: 309 RQGPRISVASFFSSSMRP-NSRVYGPMKELVSEENPPKYRDITIKEYSKIFFEKGLDGTS 366

Query: 374 GLEHFKL 380
            L + ++
Sbjct: 369 HLSNIRI 366

BLAST of MS025478 vs. TAIR 10
Match: AT1G06650.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.4e-111
Identity = 196/369 (53.12%), Postives = 259/369 (70.19%), Query Frame = 0

Query: 14  YDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSNL- 73
           +DR +ELKAFDETK GVKGLVDSG++++P+IF H  PT  + +P         SD  +L 
Sbjct: 10  FDRASELKAFDETKTGVKGLVDSGVSQVPRIFHH--PTVKLSTPKPL-----PSDLLHLK 69

Query: 74  GIPIIDLES-TAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEE 133
            IP IDL     Q+   R   I++I+ A   WGFFQ+INHG+   +  +M  G R FHE+
Sbjct: 70  TIPTIDLGGRDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQ 129

Query: 134 DDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLACREIT 193
             E+++  YSRDF +R+ L+ +NFDL+   + NWRDT SC +AP   +P+D+P  CR+I 
Sbjct: 130 SQEVRKEFYSRDF-SRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIM 189

Query: 194 AEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDH 253
            EYSK+V+ LG+FL EL SEALG+ PN L +M C  G++M SHYYPPCPEP+LT GT+ H
Sbjct: 190 MEYSKQVMNLGKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQH 249

Query: 254 SDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMA 313
           SD+SFLTVLL DQI GLQVRR   W DV  V GAL++N+GD LQLI+ND+F S+ HRV+A
Sbjct: 250 SDNSFLTVLLPDQIEGLQVRREGHWFDVPHVSGALIINIGDLLQLITNDKFISLEHRVLA 309

Query: 314 KK-IGPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNG 373
            +    R+SVACFF   + P N R+YGPI+EL+S++NP  YRETTIKDY  ++  +GL+G
Sbjct: 310 NRATRARVSVACFFTTGVRP-NPRMYGPIRELVSEENPPKYRETTIKDYATYFNAKGLDG 369

Query: 374 ISGLEHFKL 380
            S L HFK+
Sbjct: 370 TSALLHFKI 369

BLAST of MS025478 vs. TAIR 10
Match: AT5G59530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 390.2 bits (1001), Expect = 1.9e-108
Identity = 190/368 (51.63%), Postives = 253/368 (68.75%), Query Frame = 0

Query: 14  YDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSNLG 73
           +DR  E KAFD TK GVKGL+D+ +T+IP+IF     T P +  +           S+L 
Sbjct: 8   FDRYIERKAFDNTKEGVKGLIDAKITEIPRIFHVPQDTLPDKKRSV----------SDLE 67

Query: 74  IPIIDLESTAQNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEEDD 133
           IP ID  S   +  SR   +++++ A ENWGFFQ+INHG+P +V  E+ +G RRFHEE+D
Sbjct: 68  IPTIDFASVNVDTPSREAIVEKVKYAVENWGFFQVINHGVPLNVLEEIKDGVRRFHEEED 127

Query: 134 -EIKRNLYSRDFVARKVLFNTNFDLY-RGVSTNWRDTLSCVVAPRGAEPEDIPLACREIT 193
            E+K++ YS DF   K  +++NFDLY    S  WRD++SC +AP    PE++P  CR+  
Sbjct: 128 PEVKKSYYSLDFTKNKFAYSSNFDLYSSSPSLTWRDSISCYMAPDPPTPEELPETCRDAM 187

Query: 194 AEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDH 253
            EYSK VL LG  L EL SEALG+    L+ M C   ++M  HYYPPCP+P+LT G + H
Sbjct: 188 IEYSKHVLSLGDLLFELLSEALGLKSEILKSMDCLKSLLMICHYYPPCPQPDLTLGISKH 247

Query: 254 SDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMA 313
           SD+SFLTVLLQD IGGLQ+   D WVDVSP+ GALVVNVGDFLQLI+ND+F SV HRV+A
Sbjct: 248 SDNSFLTVLLQDNIGGLQILHQDSWVDVSPLPGALVVNVGDFLQLITNDKFISVEHRVLA 307

Query: 314 KKIGPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNGI 373
              GPRISVA FF  ++  EN  VYGP+KEL+S++NP  YR+TT+++Y   Y+++GL+G 
Sbjct: 308 NTRGPRISVASFFSSSI-RENSTVYGPMKELVSEENPPKYRDTTLREYSEGYFKKGLDGT 364

Query: 374 SGLEHFKL 380
           S L +F++
Sbjct: 368 SHLSNFRI 364

BLAST of MS025478 vs. TAIR 10
Match: AT2G30840.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.2e-107
Identity = 197/369 (53.39%), Postives = 258/369 (69.92%), Query Frame = 0

Query: 14  YDRQTELKAFDETKLGVKGLVDSGLTKIPKIFIHDDPTSPIESPAAAMAAASESDNSNLG 73
           YDR +E+KAFDE K+GVKGL+D+G+T+IP+IF H  P   +      ++      ++ + 
Sbjct: 5   YDRASEVKAFDELKIGVKGLLDAGVTQIPRIFHH--PHLNLTDSNLLLS------STTMV 64

Query: 74  IPIIDLESTA--QNPQSRGKTIDQIRAACENWGFFQIINHGIPSSVTAEMINGTRRFHEE 133
           IP IDL+     +   +R   I  IR A E +GFFQ+INHGI + V  +M +G R FHE+
Sbjct: 65  IPTIDLKGGVFDEYTVTRESVIAMIRDAVERFGFFQVINHGISNDVMEKMKDGIRGFHEQ 124

Query: 134 DDEIKRNLYSRDFVARKVLFNTNFDLYRGVSTNWRDTLSCVVAPRGAEPEDIPLACREIT 193
           D ++++  Y+RD V + V +N+NFDLY   S NWRDTLSC +AP   E ED+P  C EI 
Sbjct: 125 DSDVRKKFYTRD-VTKTVKYNSNFDLYSSPSANWRDTLSCFMAPDVPETEDLPDICGEIM 184

Query: 194 AEYSKRVLELGQFLMELASEALGVGPNRLREMGCGDGMVMFSHYYPPCPEPELTWGTTDH 253
            EY+KRV++LG+ + EL SEALG+ PN L+EM C  G++M SHYYPPCPEP LT+GT+ H
Sbjct: 185 LEYAKRVMKLGELIFELLSEALGLNPNHLKEMDCTKGLLMLSHYYPPCPEPGLTFGTSPH 244

Query: 254 SDSSFLTVLLQDQIGGLQVRRGDRWVDVSPVDGALVVNVGDFLQLISNDRFKSVNHRVMA 313
           SD SFLT+LLQD IGGLQVR+   WVDV PV GAL+VN+GD LQL++ND+F SV HRV+A
Sbjct: 245 SDRSFLTILLQDHIGGLQVRQNGYWVDVPPVPGALLVNLGDLLQLMTNDQFVSVEHRVLA 304

Query: 314 KK-IGPRISVACFFRCNLPPENGRVYGPIKELISDQNPAIYRETTIKDYVAHYYEQGLNG 373
            K   PRISVA FF   LP  + RVYGPIKEL+S+QN   YR+TT+ +Y +HY  +GL G
Sbjct: 305 NKGEKPRISVASFFVHPLP--SLRVYGPIKELLSEQNLPKYRDTTVTEYTSHYMARGLYG 362

Query: 374 ISGLEHFKL 380
            S L  FK+
Sbjct: 365 NSVLLDFKI 362