MS025174 (gene) Bitter gourd (TR) v1

BLAST of MS025174 vs. NCBI nr
Match: XP_022152844.1 (berberine bridge enzyme-like 27 [Momordica charantia])

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 549/554 (99.10%), Postives = 551/554 (99.46%), Query Frame = 0

Query: 1   MNFVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYS 60
           MNFVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYS
Sbjct: 1   MNFVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYS 60

Query: 61  SILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSY 120
           SILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSY
Sbjct: 61  SILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSY 120

Query: 121 VSSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV 180
           VSSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV
Sbjct: 121 VSSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV 180

Query: 181 GGFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIV 240
           GGFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIV
Sbjct: 181 GGFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIV 240

Query: 241 LAWKLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGG 300
           LAWKLKLVPVPAIVTC AINKTWDQ+VAKLVHQWQYVAPEADENLFISVTVTARNSSEGG
Sbjct: 241 LAWKLKLVPVPAIVTCIAINKTWDQSVAKLVHQWQYVAPEADENLFISVTVTARNSSEGG 300

Query: 301 GRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGEL 360
           GRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGEL
Sbjct: 301 GRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGEL 360

Query: 361 SEVLVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNE 420
           SEVLVKKPPLNSRRYKVKSDFATEPI ETAMEGIWERFKIIGGIETVKLILNPHGGKMNE
Sbjct: 361 SEVLVKKPPLNSRRYKVKSDFATEPIPETAMEGIWERFKIIGGIETVKLILNPHGGKMNE 420

Query: 421 ISETETPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCR 480
           ISET+TPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCR
Sbjct: 421 ISETKTPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCR 480

Query: 481 DLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAARRG 540
           DLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAARRG
Sbjct: 481 DLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAARRG 540

Query: 541 IQAKVSSCPDVFDL 555
           IQAKVS CPDVFDL
Sbjct: 541 IQAKVSLCPDVFDL 554

BLAST of MS025174 vs. NCBI nr
Match: XP_038894011.1 (berberine bridge enzyme-like 18 isoform X2 [Benincasa hispida])

HSP 1 Score: 788.1 bits (2034), Expect = 4.9e-224
Identity = 392/550 (71.27%), Postives = 456/550 (82.91%), Query Frame = 0

Query: 3   FVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSS-TNDSNSILIHTPSSPSYSS 62
           F  SY SFISLLLL  ++ +    G+N EDF+HCISLHSS  NDSN+ LIHTPSS SYS 
Sbjct: 4   FWSSYYSFISLLLL-LSLATLGCWGDNGEDFVHCISLHSSNNNDSNTTLIHTPSSSSYSY 63

Query: 63  ILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYV 122
           +LNF+IRN RF   +T KPVAIITPS ASHVQA+VICCK+HG QIRTRSGGHDFEG SYV
Sbjct: 64  VLNFSIRNLRFSEPETPKPVAIITPSHASHVQAAVICCKSHGLQIRTRSGGHDFEGRSYV 123

Query: 123 SSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVG 182
           ++VPFVLIDLINLNS+T+DT+D +AWVQSGATIG+LYYRI EK++TLGFP    P +GVG
Sbjct: 124 ANVPFVLIDLINLNSITIDTEDESAWVQSGATIGKLYYRIAEKSQTLGFPAGFAPTIGVG 183

Query: 183 GFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVL 242
           G+LSGGGFG MVRKYGLA DNVVDAY VDANG++ DR SMG+DLFWAIRGGGGGSFGIVL
Sbjct: 184 GYLSGGGFGMMVRKYGLAVDNVVDAYVVDANGRIFDRHSMGEDLFWAIRGGGGGSFGIVL 243

Query: 243 AWKLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGGG 302
           AWKLKLVPVP IVT FA++KTWDQN A L+H+WQYVAPE DE+LFI+  VTA NSS+ GG
Sbjct: 244 AWKLKLVPVPPIVTSFALHKTWDQNAADLIHRWQYVAPEVDEDLFITAWVTASNSSKEGG 303

Query: 303 RMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGELS 362
           R+M A FFSLFLGKA++LL +M+K+FPELGLKKEDC+E SWIES A +  ASGFVS    
Sbjct: 304 RIMEASFFSLFLGKADKLLSLMQKTFPELGLKKEDCLEMSWIESMAFM--ASGFVSANNV 363

Query: 363 EVLVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEI 422
           ++L+ + PL++ RYK KSD+AT+PI +TA+EG+WERFK I  +ETV+L+L P GGKMNEI
Sbjct: 364 KLLLDRTPLSNDRYKTKSDYATKPISKTALEGMWERFK-IEELETVQLMLIPFGGKMNEI 423

Query: 423 SETETPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCRD 482
           SETETP  YRAGYPI+I YYV W+ PDAD RHL WT+EL++YMTPFVSKSPR  Y N +D
Sbjct: 424 SETETPFAYRAGYPIQIGYYVTWQDPDADLRHLKWTQELHDYMTPFVSKSPRGAYINYKD 483

Query: 483 LDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAARRGI 542
           LDIG N E+GI TSYEEA VWG  YFGNNFE+LVEVK KVDP NFF HEQSIPP      
Sbjct: 484 LDIGTNKEDGIPTSYEEAGVWGHPYFGNNFERLVEVKMKVDPFNFFRHEQSIPP------ 542

Query: 543 QAKVSSCPDV 552
            AK++SCPDV
Sbjct: 544 -AKMASCPDV 542

BLAST of MS025174 vs. NCBI nr
Match: XP_038895067.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 784.6 bits (2025), Expect = 5.4e-223
Identity = 388/537 (72.25%), Postives = 444/537 (82.68%), Query Frame = 0

Query: 3   FVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYSSI 62
           F   Y  FI LLL + A L     G N+EDF+HCI LHS+ NDSNS LIHTPSS SYS +
Sbjct: 4   FCSCYYGFIFLLLFSLATLGCW--GYNMEDFVHCIYLHSNNNDSNSTLIHTPSSSSYSYV 63

Query: 63  LNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYVS 122
           LNF+IRN RF   +T KPVAIITPS AS VQA+VICCK+HGFQIRTRSGGHDFEG SYV+
Sbjct: 64  LNFSIRNLRFSEPETPKPVAIITPSHASQVQAAVICCKSHGFQIRTRSGGHDFEGSSYVA 123

Query: 123 SVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGG 182
           +VPFVLIDLINLNS+T+DT+D +AWVQSGAT+GELYYRIGEK++TLGFP   VP +G+GG
Sbjct: 124 NVPFVLIDLINLNSITIDTEDESAWVQSGATVGELYYRIGEKSRTLGFPAGFVPTIGIGG 183

Query: 183 FLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLA 242
           FLSGGGFG MVRKYGLAADNVVDAY VDANG + DR SMG+DLFWAIRGGGGGSFGIVLA
Sbjct: 184 FLSGGGFGMMVRKYGLAADNVVDAYVVDANGSVFDRYSMGEDLFWAIRGGGGGSFGIVLA 243

Query: 243 WKLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGGGR 302
           WKLKLVPVP  VT F+++KTWDQN A L+H+WQYVAPE D++LFIS  VTA NSS+G GR
Sbjct: 244 WKLKLVPVPPTVTSFSLSKTWDQNAADLIHRWQYVAPEVDQDLFISAWVTAFNSSDGSGR 303

Query: 303 MMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGELSE 362
           +M A FFSL+LGKA ELL +MEK+FPELGLKKEDC+E SW+ES A    ASGFVS +  E
Sbjct: 304 IMEASFFSLYLGKATELLSLMEKTFPELGLKKEDCLEMSWVESMAF--SASGFVSAKSLE 363

Query: 363 VLVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEIS 422
           +L+ + PL++ R K+KSD+ TEPI ETA+EG+WERFK    +E V+ IL P GGKMNEIS
Sbjct: 364 LLLNRSPLDNGRQKIKSDYPTEPISETALEGMWERFK-NEELEKVQFILIPFGGKMNEIS 423

Query: 423 ETETPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCRDL 482
           E+ETPS +RAGYPI I YYV W+ PDAD+RHL WTRELYNYMTPFVSKSPRA YAN RDL
Sbjct: 424 ESETPSAHRAGYPIHIGYYVKWQHPDADSRHLEWTRELYNYMTPFVSKSPRAAYANYRDL 483

Query: 483 DIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAARR 540
           DIG NNE+ I TSYEEASVWG +YFGNNFE+L+EVK KVD  NFF HEQSIPPA  R
Sbjct: 484 DIGTNNEDRILTSYEEASVWGHQYFGNNFERLMEVKAKVDQFNFFRHEQSIPPAPPR 535

BLAST of MS025174 vs. NCBI nr
Match: XP_038894010.1 (berberine bridge enzyme-like 18 isoform X1 [Benincasa hispida])

HSP 1 Score: 783.5 bits (2022), Expect = 1.2e-222
Identity = 392/551 (71.14%), Postives = 456/551 (82.76%), Query Frame = 0

Query: 3   FVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSS-TNDSNSILIHTPSSPSYSS 62
           F  SY SFISLLLL  ++ +    G+N EDF+HCISLHSS  NDSN+ LIHTPSS SYS 
Sbjct: 4   FWSSYYSFISLLLL-LSLATLGCWGDNGEDFVHCISLHSSNNNDSNTTLIHTPSSSSYSY 63

Query: 63  ILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYV 122
           +LNF+IRN RF   +T KPVAIITPS ASHVQA+VICCK+HG QIRTRSGGHDFEG SYV
Sbjct: 64  VLNFSIRNLRFSEPETPKPVAIITPSHASHVQAAVICCKSHGLQIRTRSGGHDFEGRSYV 123

Query: 123 SSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVG 182
           ++VPFVLIDLINLNS+T+DT+D +AWVQSGATIG+LYYRI EK++TLGFP    P +GVG
Sbjct: 124 ANVPFVLIDLINLNSITIDTEDESAWVQSGATIGKLYYRIAEKSQTLGFPAGFAPTIGVG 183

Query: 183 GFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVL 242
           G+LSGGGFG MVRKYGLA DNVVDAY VDANG++ DR SMG+DLFWAIRGGGGGSFGIVL
Sbjct: 184 GYLSGGGFGMMVRKYGLAVDNVVDAYVVDANGRIFDRHSMGEDLFWAIRGGGGGSFGIVL 243

Query: 243 AWKLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVT-ARNSSEGG 302
           AWKLKLVPVP IVT FA++KTWDQN A L+H+WQYVAPE DE+LFI+  VT A NSS+ G
Sbjct: 244 AWKLKLVPVPPIVTSFALHKTWDQNAADLIHRWQYVAPEVDEDLFITAWVTAASNSSKEG 303

Query: 303 GRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGEL 362
           GR+M A FFSLFLGKA++LL +M+K+FPELGLKKEDC+E SWIES A +  ASGFVS   
Sbjct: 304 GRIMEASFFSLFLGKADKLLSLMQKTFPELGLKKEDCLEMSWIESMAFM--ASGFVSANN 363

Query: 363 SEVLVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNE 422
            ++L+ + PL++ RYK KSD+AT+PI +TA+EG+WERFK I  +ETV+L+L P GGKMNE
Sbjct: 364 VKLLLDRTPLSNDRYKTKSDYATKPISKTALEGMWERFK-IEELETVQLMLIPFGGKMNE 423

Query: 423 ISETETPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCR 482
           ISETETP  YRAGYPI+I YYV W+ PDAD RHL WT+EL++YMTPFVSKSPR  Y N +
Sbjct: 424 ISETETPFAYRAGYPIQIGYYVTWQDPDADLRHLKWTQELHDYMTPFVSKSPRGAYINYK 483

Query: 483 DLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAARRG 542
           DLDIG N E+GI TSYEEA VWG  YFGNNFE+LVEVK KVDP NFF HEQSIPP     
Sbjct: 484 DLDIGTNKEDGIPTSYEEAGVWGHPYFGNNFERLVEVKMKVDPFNFFRHEQSIPP----- 543

Query: 543 IQAKVSSCPDV 552
             AK++SCPDV
Sbjct: 544 --AKMASCPDV 543

BLAST of MS025174 vs. NCBI nr
Match: KAG6583541.1 (hypothetical protein SDJN03_19473, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 773.9 bits (1997), Expect = 9.6e-220
Identity = 385/551 (69.87%), Postives = 451/551 (81.85%), Query Frame = 0

Query: 1   MNFVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYS 60
           M    S  SFISLLLL++A     SD  NL+DFLHCISLHSS + SNS LIHTPSS SY 
Sbjct: 1   MKIYWSNFSFISLLLLSFASWGCGSD--NLQDFLHCISLHSSNHHSNSTLIHTPSSSSYP 60

Query: 61  SILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSY 120
            +LNF+IRN RF  S T KPVAI+TP Q S VQA+VICCK HG QIRTRSGGHDFEG SY
Sbjct: 61  YVLNFSIRNLRFAESHTPKPVAIVTPWQPSEVQATVICCKTHGLQIRTRSGGHDFEGRSY 120

Query: 121 VSSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV 180
           VS+VPFVLIDLINLNS+T+D  + TAW QSGAT+GELYY+IGEK++TL FP     ++G+
Sbjct: 121 VSNVPFVLIDLINLNSITIDVDNETAWAQSGATVGELYYKIGEKSRTLAFPAGFTASIGL 180

Query: 181 GGFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIV 240
           GGF SGGG G +VRKYGLA DNVVDAY VDANGKL DR SMG+DLFWAIRGGGGGSFGIV
Sbjct: 181 GGFFSGGGLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIV 240

Query: 241 LAWKLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGG 300
           LAWKLKLV VP  VT FA++K+WD N AKL+H+WQYVAP+ DE+LFI+  VTA NSS+ G
Sbjct: 241 LAWKLKLVRVPPTVTSFAVHKSWDLNAAKLIHRWQYVAPKVDEDLFITARVTASNSSKEG 300

Query: 301 GRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGEL 360
           GR+M+A FFSLFLGKA EL+ +M+K+FPEL LKKEDC+E SW+E+ A    AS +VS E 
Sbjct: 301 GRVMKASFFSLFLGKAAELVSLMKKAFPELELKKEDCLEISWVEAMAF--SASEYVSPEN 360

Query: 361 SEVLVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNE 420
            E+L+ + PL + RYK+KSD+AT+PI ETA+ GIWERFK I  +ETV+LIL P GGKMNE
Sbjct: 361 LELLLDRTPLITGRYKMKSDYATKPISETALNGIWERFK-IEELETVQLILIPFGGKMNE 420

Query: 421 ISETETPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCR 480
           ISET+TP+P+RA +PI I YYV W +PDAD+RHL WTREL++YMTPFVS+SPRA Y N +
Sbjct: 421 ISETDTPNPHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYK 480

Query: 481 DLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAARRG 540
           DLDIGRNNE+GI TSY+EASVWG +YFG NFE+LVEVKTKVDP NFF HEQSIPPA   G
Sbjct: 481 DLDIGRNNEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPPAG--G 540

Query: 541 IQAKVSSCPDV 552
           IQAK++SCPDV
Sbjct: 541 IQAKMASCPDV 544

BLAST of MS025174 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.0e-139
Identity = 260/536 (48.51%), Postives = 353/536 (65.86%), Query Frame = 0

Query: 8   SSFISLLLLAYAIL---SSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYSSILN 67
           S FI  LLL  ++    S S+   N E+FL C+S     N+ +S +IHT   PSY SILN
Sbjct: 9   SLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLS--HRINEDDSRIIHTSKDPSYFSILN 68

Query: 68  FNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYVS-S 127
            +I+NPRF+  +T KPV+IITP QA+ VQ+++ C + HG  IRTRSGGHD+EGLSY++ S
Sbjct: 69  SSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKS 128

Query: 128 VPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGF 187
            PFV+IDL NL S+T+D  + T WVQSGATIGELYY IG+ +K+L FP  + P VG+GG 
Sbjct: 129 RPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQ 188

Query: 188 LSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLAW 247
             GGG+G ++RKYGL+ADNV+DA+ VDANG   DR+ MG+D FWAIRGGGG SF +VL+W
Sbjct: 189 FGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSW 248

Query: 248 KLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGGGRM 307
           K++L+ VP++VT F + KT ++    ++++WQY+A +   +LFI   +            
Sbjct: 249 KIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKETE------- 308

Query: 308 MRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGELSEV 367
           + A F  L+LG   +LL +M+  FPELGL+  +C E SWIES         F+ GE  E+
Sbjct: 309 VYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL------WFIKGESMEI 368

Query: 368 LVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEISE 427
           L K+    SR +K K DF  EPI +TA++ +W RF+        K+IL P GGKM+EI++
Sbjct: 369 LAKR-KRTSRSFKGKDDFIEEPIPKTAIQYLWRRFE-APEARLAKIILTPFGGKMSEIAD 428

Query: 428 TETPSPYRAGYPIKIFYYVLW-EQPDAD----ARHLGWTRELYNYMTPFVSKSPRAVYAN 487
            E P P+R G   +I Y   W E+ D +     ++L W   +Y +MTP+VSKSPR  Y N
Sbjct: 429 NEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVN 488

Query: 488 CRDLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIP 535
            RD+D+G      + T YEEA VWG+KYF NNF++LV VKT VDP++FF  EQSIP
Sbjct: 489 FRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MS025174 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 6.2e-137
Identity = 241/516 (46.71%), Postives = 337/516 (65.31%), Query Frame = 0

Query: 25  SDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYSSILNFNIRNPRFWGSDTHKPVAII 84
           S  N  E+FL C S H   N +N  L++T     Y SILN  I+N RF    T KP+ I+
Sbjct: 26  SIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIV 85

Query: 85  TPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYVSSVPFVLIDLINLNSVTVDTKDS 144
           TPS  SH+QA+++C K  G QIRTRSGGHD EG+SY+S VPFV++DL N++S+ +D    
Sbjct: 86  TPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQ 145

Query: 145 TAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSGGGFGPMVRKYGLAADNVV 204
           TAWV++GAT+GE+YY I EK + L FPG   P VGVGG  SGGG+G ++R YGLAADN++
Sbjct: 146 TAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNII 205

Query: 205 DAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTCFAINKTWD 264
           DA+ V+ +GK+ DRKSMG+DLFWAIRGGGG +FGI+ AWK+KLV VP+  T F++ K  +
Sbjct: 206 DAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNME 265

Query: 265 -QNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGGGR---MMRALFFSLFLGKAEELL 324
              + KL ++WQ +A + D++L +      +N ++  G+    +   F S+F G  + L+
Sbjct: 266 IHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLV 325

Query: 325 YIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGEL-SEVLVKKPPLNSRRYKVKS 384
            +M KSFPELG+KK DC E SWI++T   S    F +     E+L+ +       + +K 
Sbjct: 326 DLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKL 385

Query: 385 DFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEISETETPSPYRAGYPIKIF 444
           D+  +PI ETAM  I E+      +     +L P+GG M EISE+  P P+RAG   +++
Sbjct: 386 DYVKKPIPETAMVKILEKL-YEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELW 445

Query: 445 YYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCRDLDIGRNNEEGISTSYEEA 504
           Y   WE+ + + +H+ W R +YN+ TP+VS++PR  Y N RDLD+G+ N      +Y +A
Sbjct: 446 YTASWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHAS-PNNYTQA 505

Query: 505 SVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPP 536
            +WG KYFG NF +LV+VKTKVDP NFF +EQSIPP
Sbjct: 506 RIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPP 539

BLAST of MS025174 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 6.9e-136
Identity = 257/536 (47.95%), Postives = 350/536 (65.30%), Query Frame = 0

Query: 7   YSSFISLLLLAYAILSS--SSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYSSILN 66
           +SSF+  +LL    +S   S+ G+N EDFL C+S   + N     +IHT    S+ SIL+
Sbjct: 3   FSSFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILD 62

Query: 67  FNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYVS-S 126
            +I+NPRF  S+T KPV+IITP +AS VQ  + C + HG  +RTRS GH +EGLSY++ +
Sbjct: 63  SSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYN 122

Query: 127 VPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGF 186
            PF +IDL NL S+++D  + T WVQ+GAT GELYY IG+ TK+L FP  + P VGVGG 
Sbjct: 123 KPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQ 182

Query: 187 LSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLAW 246
            SGGG+G ++RKYGLAADN++DA  VDA+G++ DR++MG+D FWAIRGGGG SFG++L+W
Sbjct: 183 FSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSW 242

Query: 247 KLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGGGRM 306
           K+KLV VP+ +T F + KT  +   +++ +WQY A +  ++LFI  T+   N +      
Sbjct: 243 KVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKN-----A 302

Query: 307 MRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGELSEV 366
           + ALF  L++G    LL +ME+ FPELGL+KE C E SWIES  +L  A  F  GE   V
Sbjct: 303 VHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIES--VLWFAD-FPKGESLGV 362

Query: 367 LVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEISE 426
           L  +    S  +K K DF  EPI E A++ IW R +        K+IL P GGKM+E++E
Sbjct: 363 LTNR-ERTSLSFKGKDDFVQEPIPEAAIQEIWRRLE-APEARLGKIILTPFGGKMSEMAE 422

Query: 427 TETPSPYRAGYPIKIFYYVLW-EQPDADA----RHLGWTRELYNYMTPFVSKSPRAVYAN 486
            ETP P+R G   +I Y   W E+ D +     ++L W   +Y +MTP+VSKSPR  Y N
Sbjct: 423 YETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVN 482

Query: 487 CRDLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIP 535
            +D+D+G    +   T YEE   WG+KYF NNFE+LV VKT+VDP +FF  EQSIP
Sbjct: 483 FKDMDLGMYLGKK-KTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of MS025174 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.5e-135
Identity = 239/516 (46.32%), Postives = 335/516 (64.92%), Query Frame = 0

Query: 25  SDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYSSILNFNIRNPRFWGSDTHKPVAII 84
           S  N  E+FL C S +   N +N   I+T     Y S+LN  I+N RF    T KP+ I+
Sbjct: 26  SIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIV 85

Query: 85  TPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYVSSVPFVLIDLINLNSVTVDTKDS 144
           TPS  SH+QAS++C K  G QIRTRSGGHD EGLSY+S VPF ++DL N+++V VD    
Sbjct: 86  TPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQ 145

Query: 145 TAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSGGGFGPMVRKYGLAADNVV 204
           TAWV++GAT+GE+YY I E  +   FPG   P VGVGG  SGGG+G ++R YGLAADN++
Sbjct: 146 TAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNII 205

Query: 205 DAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLAWKLKLVPVPAIVTCFAINKTWD 264
           DA+ V+ +GK+ DRKSMG+DLFWAIRGGGG +FGI+ AWK+KLV VP+  T F++ K  +
Sbjct: 206 DAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNME 265

Query: 265 -QNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGGGR---MMRALFFSLFLGKAEELL 324
              + KL ++WQ +A + D++L ++     RN ++  G+    +   F S+FLG  + L+
Sbjct: 266 IHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLV 325

Query: 325 YIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGEL-SEVLVKKPPLNSRRYKVKS 384
            +M KSFPELG+KK DC E SWI++T   S    + +     E+L+ +       + +K 
Sbjct: 326 DLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKL 385

Query: 385 DFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEISETETPSPYRAGYPIKIF 444
           D+  + I ETAM  I E+      +     +L P+GG M+EISE+  P P+RAG   +++
Sbjct: 386 DYVKKLIPETAMVKILEKL-YEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELW 445

Query: 445 YYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCRDLDIGRNNEEGISTSYEEA 504
           Y   WE+ + + +H+ W R +YN+ TP+VS++PR  Y N RDLD+G+ N E    +Y +A
Sbjct: 446 YTATWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPES-PNNYTQA 505

Query: 505 SVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPP 536
            +WG KYFG NF +LV+VKTK DP NFF +EQSIPP
Sbjct: 506 RIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPP 539

BLAST of MS025174 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 4.9e-134
Identity = 255/540 (47.22%), Postives = 349/540 (64.63%), Query Frame = 0

Query: 1   MNFVGSYSSFISLLLLAYAILS----SSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSS 60
           M F   +SS +     +  +LS     S++ +N   FL C+SL  + ++  S +IHTP+ 
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPND 60

Query: 61  PSYSSILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFE 120
            S+SS+L  +I+N RF   D  KPV I+TP Q S VQ++V C +  G  IRTRSGGHD+E
Sbjct: 61  TSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYE 120

Query: 121 GLSYVSSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVP 180
           GLSYV+  PFV++DL NL S+TVD  + + WVQ+GATIGELYY IG+K +TL FP  V P
Sbjct: 121 GLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCP 180

Query: 181 NVGVGGFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGS 240
            VGVGG  SGGG+G ++RK+GLAAD+V+DA  VDA G++ +R+ MG+D FWAIRGGGG S
Sbjct: 181 TVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSS 240

Query: 241 FGIVLAWKLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNS 300
           F +VL+WK+ L+ VP+ VT F + K  +Q+  K++H+WQ+VA +  ++LFI V +    +
Sbjct: 241 FCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKN 300

Query: 301 SEGGGRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFV 360
                 M+RA F  L+LG  + LL ++ K FPELGL+++DC E SWIES     I    +
Sbjct: 301 ------MVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESV----IWFAEL 360

Query: 361 SGELSEVLVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGG 420
             E   VL K+    S  +K KSDF  EP+ +TA+  +W R +     E  +LI  P GG
Sbjct: 361 GEEPINVLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQ-EPEAEHAQLIFTPFGG 420

Query: 421 KMNEISETETPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVY 480
           KM+EI++ ETP P+R G   +I Y   W + D   +++ W   +Y+ M+ FV+KSPR  Y
Sbjct: 421 KMSEIADYETPFPHRKGNIYEIQYLNYW-RGDVKEKYMRWVERVYDDMSEFVAKSPRGAY 480

Query: 481 ANCRDLDIGRNNEEGISTS-YEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPP 536
            N RDLD+G     G+  S YEE   WG+KYF NNFE+LV VKT VDP +FF  EQSIPP
Sbjct: 481 INLRDLDLGM--YVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of MS025174 vs. ExPASy TrEMBL
Match: A0A6J1DHA5 (berberine bridge enzyme-like 27 OS=Momordica charantia OX=3673 GN=LOC111020467 PE=3 SV=1)

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 549/554 (99.10%), Postives = 551/554 (99.46%), Query Frame = 0

Query: 1   MNFVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYS 60
           MNFVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYS
Sbjct: 1   MNFVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYS 60

Query: 61  SILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSY 120
           SILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSY
Sbjct: 61  SILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSY 120

Query: 121 VSSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV 180
           VSSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV
Sbjct: 121 VSSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV 180

Query: 181 GGFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIV 240
           GGFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIV
Sbjct: 181 GGFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIV 240

Query: 241 LAWKLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGG 300
           LAWKLKLVPVPAIVTC AINKTWDQ+VAKLVHQWQYVAPEADENLFISVTVTARNSSEGG
Sbjct: 241 LAWKLKLVPVPAIVTCIAINKTWDQSVAKLVHQWQYVAPEADENLFISVTVTARNSSEGG 300

Query: 301 GRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGEL 360
           GRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGEL
Sbjct: 301 GRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGEL 360

Query: 361 SEVLVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNE 420
           SEVLVKKPPLNSRRYKVKSDFATEPI ETAMEGIWERFKIIGGIETVKLILNPHGGKMNE
Sbjct: 361 SEVLVKKPPLNSRRYKVKSDFATEPIPETAMEGIWERFKIIGGIETVKLILNPHGGKMNE 420

Query: 421 ISETETPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCR 480
           ISET+TPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCR
Sbjct: 421 ISETKTPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCR 480

Query: 481 DLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAARRG 540
           DLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAARRG
Sbjct: 481 DLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAARRG 540

Query: 541 IQAKVSSCPDVFDL 555
           IQAKVS CPDVFDL
Sbjct: 541 IQAKVSLCPDVFDL 554

BLAST of MS025174 vs. ExPASy TrEMBL
Match: A0A6J1HP64 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111464765 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 5.1e-219
Identity = 384/545 (70.46%), Postives = 449/545 (82.39%), Query Frame = 0

Query: 10  FISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYSSILNFNIRN 69
           FISLLLL++A     SD  NL+DFLHCISLHSS + SNS LIHTPSS SY  +LNF+IRN
Sbjct: 31  FISLLLLSFASWVCGSD--NLQDFLHCISLHSSNHHSNSTLIHTPSSSSYPHVLNFSIRN 90

Query: 70  PRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYVSSVPFVLI 129
            RF  S T KPVAI+TP Q S VQA+VICCK HG QIRTRSGGHDFEG SYVS+VPFVLI
Sbjct: 91  LRFAESHTPKPVAIVTPWQPSEVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNVPFVLI 150

Query: 130 DLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSGGGF 189
           DLINLNS+T+D  + TAW QSGAT+GELYY+IGEK++TL FP     ++G+GGF SGGG 
Sbjct: 151 DLINLNSITIDVDNETAWAQSGATVGELYYKIGEKSRTLAFPAGFTASIGLGGFFSGGGL 210

Query: 190 GPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLAWKLKLVP 249
           G +VRKYGLA DNVVDAY VDANGKL DR SMG+DLFWAIRGGGGGSFGIVLAWKLKLV 
Sbjct: 211 GMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVR 270

Query: 250 VPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSS--EGGGRMMRAL 309
           VP  VT FA++K+WD N AKL+H+WQYVAP+ DE+LFI+  VTA NSS  EGGGR+M+A 
Sbjct: 271 VPPTVTSFAVHKSWDLNAAKLIHRWQYVAPKVDEDLFITAWVTASNSSEGEGGGRVMKAS 330

Query: 310 FFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGELSEVLVKK 369
           FFSLFLGKA EL+ +M+K+FPEL LKKEDC+E SW+E+ A    AS +VS E  E+L+ +
Sbjct: 331 FFSLFLGKAAELVSLMKKAFPELELKKEDCLEISWVEAMAF--SASEYVSPENLELLLDR 390

Query: 370 PPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEISETETP 429
            PL + RYK+KSD+AT+PI ETA+ GIWERFK I  +ETV+LIL P GGKMNEISET TP
Sbjct: 391 TPLITGRYKMKSDYATKPISETALNGIWERFK-IEELETVQLILIPFGGKMNEISETATP 450

Query: 430 SPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCRDLDIGRN 489
           +P+RA +PI I YYV W +PDAD+RHL WTREL++YMTPFVS+SPRA Y N +DLDIGRN
Sbjct: 451 NPHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGRN 510

Query: 490 NEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAARRG-IQAKVS 549
           NE+GI TSY+EASVWG +YFG NFE+LVEVKTKVDP NFF HE+SIPPAA  G IQAK++
Sbjct: 511 NEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFQHEESIPPAAPMGVIQAKMA 570

Query: 550 SCPDV 552
           SCPDV
Sbjct: 571 SCPDV 570

BLAST of MS025174 vs. ExPASy TrEMBL
Match: A0A6J1HYZ7 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469396 PE=3 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 1.1e-218
Identity = 382/543 (70.35%), Postives = 446/543 (82.14%), Query Frame = 0

Query: 9   SFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYSSILNFNIR 68
           +FISLLLL++A     SD  NLEDFLHCISLHSS   SNS LIHTPSS SY  +LNF+IR
Sbjct: 25  NFISLLLLSFASWGFGSD--NLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIR 84

Query: 69  NPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYVSSVPFVL 128
           N RF    T KPVAI+TP QAS VQA+VICCK HG QIRTRSGGHDFEG SYVS++PFVL
Sbjct: 85  NLRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVL 144

Query: 129 IDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSGGG 188
           IDLINLNS+T+D  D TAW QSGAT+GELYY+IGEK++TL FP  +  ++G+GGF SGGG
Sbjct: 145 IDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGG 204

Query: 189 FGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLAWKLKLV 248
            G +VRKYGLA DNVVDAY VDANGKL DR SMG+DLFWAIRGGGGGSFGIVLAWKLKLV
Sbjct: 205 LGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLV 264

Query: 249 PVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGGGRMMRALF 308
            VP  VT FA++K+WD N AKL+H WQYVA E DE+LFI+  VTA NSS+ GGR+M+A F
Sbjct: 265 RVPPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASF 324

Query: 309 FSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGELSEVLVKKP 368
           FSLFLGKA EL+ +MEK+FPEL LKKEDC+E SW+E+ A    AS +VS E  E+L+ + 
Sbjct: 325 FSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAF--SASEYVSPENLELLLDRT 384

Query: 369 PLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEISETETPS 428
            L + RYK+KSD+AT+PI ETA+ GIWERFK I  +ETV+LIL P GGKMNEISET+TP+
Sbjct: 385 SLITGRYKMKSDYATKPISETALNGIWERFK-IEELETVQLILIPFGGKMNEISETDTPN 444

Query: 429 PYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCRDLDIGRNN 488
           P+RA +PI I YYV W +PDAD+RHL WTREL++YMTPFVS+SPRA Y N +DLDIG+NN
Sbjct: 445 PHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNN 504

Query: 489 EEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAA-RRGIQAKVSS 548
           E+GI TSY+EASVWG +YFG NFE+LVEVKTKVDP NFF HEQSIPPAA   GIQAK++S
Sbjct: 505 EDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPPAAPMGGIQAKMAS 562

Query: 549 CPD 551
           CPD
Sbjct: 565 CPD 562

BLAST of MS025174 vs. ExPASy TrEMBL
Match: A0A6J1I038 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469264 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 2.2e-217
Identity = 378/544 (69.49%), Postives = 449/544 (82.54%), Query Frame = 0

Query: 10  FISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYSSILNFNIRN 69
           F+SL+ +++A     SD  NL+DFLHCISLHSS ++SNS LIHTPSS SY  +LNF+IRN
Sbjct: 17  FVSLVFVSFASWGCGSD--NLQDFLHCISLHSSNHNSNSTLIHTPSSSSYLYVLNFSIRN 76

Query: 70  PRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYVSSVPFVLI 129
            RF  SDT KPVAI+TP QAS VQA+VICCK HG QIRTRSGG DFEG SYVS+VPFVLI
Sbjct: 77  LRFAESDTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGQDFEGRSYVSNVPFVLI 136

Query: 130 DLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSGGGF 189
           DLINLNS+T+DT+D TAW QSGAT+GELYY+IG+K++TL FP  +V N+G+GGF SGGGF
Sbjct: 137 DLINLNSITIDTEDETAWAQSGATVGELYYKIGQKSRTLAFPAGLVANLGLGGFFSGGGF 196

Query: 190 GPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLAWKLKLVP 249
           G ++RKYGLA DNVVDA  VDANGKL DR SMG+DLFWAIRGGGGGSFGIVL WKLKLV 
Sbjct: 197 GMLLRKYGLAVDNVVDAQLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLKLVR 256

Query: 250 VPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGGGRMMRALFF 309
           VP  VT FA++K+WD N AKL+HQWQYVAPE DE+LFI+  VTA NSS+GGGR+M+A FF
Sbjct: 257 VPPTVTSFAVHKSWDLNAAKLIHQWQYVAPEVDEDLFITAWVTASNSSDGGGRIMKASFF 316

Query: 310 SLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGELSEVLV-KKP 369
           +LFLG+A EL+ +M K+FPEL L +EDC+E SW+ES A    AS FVS E  E+L+ +  
Sbjct: 317 ALFLGEAAELVSLMAKAFPELELTREDCLEMSWVESMAF--SASVFVSAENLELLLDRTT 376

Query: 370 PLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEISETETPS 429
            L++ RYK+KSD+ATEPI ETA+ GIWERFK I  +ET++LIL P GGKMNEISET+TP+
Sbjct: 377 QLSNGRYKIKSDYATEPISETALNGIWERFK-IEELETLQLILIPFGGKMNEISETDTPN 436

Query: 430 PYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCRDLDIGRNN 489
           P+RA +PI I YY  W +PDAD+RHL WTREL++YMTPFVSKSPRA Y N +DLDIGRNN
Sbjct: 437 PHRARHPIHIGYYATWHRPDADSRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGRNN 496

Query: 490 EEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAAR-RGIQAKVSS 549
           E+GI TSYEEAS+WG +YFG NFE+LVEVKTK DP NFF HEQSIPP A  RGIQAK++S
Sbjct: 497 EDGIPTSYEEASIWGHQYFGINFERLVEVKTKADPFNFFRHEQSIPPVAPIRGIQAKMAS 555

Query: 550 CPDV 552
           C D+
Sbjct: 557 CSDM 555

BLAST of MS025174 vs. ExPASy TrEMBL
Match: A0A6J1EVN7 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111438506 PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 2.4e-216
Identity = 379/545 (69.54%), Postives = 446/545 (81.83%), Query Frame = 0

Query: 9   SFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYSSILNFNIR 68
           SFISL+ +++A     SD  NL+DFLHCISLHSS ++SNS LIHTPSS SY  +LNF+IR
Sbjct: 16  SFISLVFVSFASWGCGSD--NLQDFLHCISLHSSNHNSNSTLIHTPSSSSYLYVLNFSIR 75

Query: 69  NPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYVSSVPFVL 128
           N RF  SDT KPVAI+TP QAS VQ +VICCK HG QIRTRSGG DFEG SYVS+VPFVL
Sbjct: 76  NLRFAESDTPKPVAIVTPWQASEVQTTVICCKTHGLQIRTRSGGQDFEGRSYVSNVPFVL 135

Query: 129 IDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSGGG 188
           IDL NLNS+T+DT+D TAW QSGAT+GELYY+IGEK++TL FP  +V N+G+GGF SGGG
Sbjct: 136 IDLTNLNSITIDTEDETAWAQSGATVGELYYKIGEKSRTLAFPAGLVANLGLGGFFSGGG 195

Query: 189 FGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLAWKLKLV 248
           FG +VRKYGLA DNVVDA  VDANGKL DR SMG+DLFWAIRGGGGGSFGIVL WKLKLV
Sbjct: 196 FGMLVRKYGLAVDNVVDAQLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLKLV 255

Query: 249 PVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGGGRMMRALF 308
            VP  VT FA++K+WD N AKL+H+WQYVAPEADE+LFI+  V A NSS+GGGR+M+A F
Sbjct: 256 RVPPTVTSFAVHKSWDLNAAKLIHRWQYVAPEADEDLFITAWVIASNSSDGGGRIMKASF 315

Query: 309 FSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGELSEVLV-KK 368
           FSLFLG+A EL+ +M K+FPEL L KEDC+E SW+ES A    AS FVS E  E+L+ + 
Sbjct: 316 FSLFLGEAAELVSLMAKAFPELELTKEDCLEMSWVESMAF--SASVFVSAESLELLLDRT 375

Query: 369 PPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEISETETP 428
             L++ RYK+KSD+ATEPI ETA+ GIWERFK I  +ET++L+L P GGKMNEISET+TP
Sbjct: 376 TQLSNGRYKIKSDYATEPISETALNGIWERFK-IEELETLQLVLIPFGGKMNEISETDTP 435

Query: 429 SPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCRDLDIGRN 488
           +P+RA +PI I YY  W +PDAD+RHL WTREL++YMTPFVSKSPRA Y N +DLDIGRN
Sbjct: 436 NPHRARHPIHIGYYATWHRPDADSRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGRN 495

Query: 489 NEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPPAAR-RGIQAKVS 548
           NE+GI TSYEEAS+WG +YFG NFE+LV VKTK DP NFF HEQSIPP A   GIQAK++
Sbjct: 496 NEDGIPTSYEEASIWGHQYFGINFERLVAVKTKADPFNFFRHEQSIPPVAPIGGIQAKMA 555

Query: 549 SCPDV 552
           SC DV
Sbjct: 556 SCSDV 555

BLAST of MS025174 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 498.4 bits (1282), Expect = 7.3e-141
Identity = 260/536 (48.51%), Postives = 353/536 (65.86%), Query Frame = 0

Query: 8   SSFISLLLLAYAIL---SSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYSSILN 67
           S FI  LLL  ++    S S+   N E+FL C+S     N+ +S +IHT   PSY SILN
Sbjct: 9   SLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLS--HRINEDDSRIIHTSKDPSYFSILN 68

Query: 68  FNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYVS-S 127
            +I+NPRF+  +T KPV+IITP QA+ VQ+++ C + HG  IRTRSGGHD+EGLSY++ S
Sbjct: 69  SSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKS 128

Query: 128 VPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGF 187
            PFV+IDL NL S+T+D  + T WVQSGATIGELYY IG+ +K+L FP  + P VG+GG 
Sbjct: 129 RPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQ 188

Query: 188 LSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLAW 247
             GGG+G ++RKYGL+ADNV+DA+ VDANG   DR+ MG+D FWAIRGGGG SF +VL+W
Sbjct: 189 FGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSW 248

Query: 248 KLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGGGRM 307
           K++L+ VP++VT F + KT ++    ++++WQY+A +   +LFI   +            
Sbjct: 249 KIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKETE------- 308

Query: 308 MRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGELSEV 367
           + A F  L+LG   +LL +M+  FPELGL+  +C E SWIES         F+ GE  E+
Sbjct: 309 VYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL------WFIKGESMEI 368

Query: 368 LVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEISE 427
           L K+    SR +K K DF  EPI +TA++ +W RF+        K+IL P GGKM+EI++
Sbjct: 369 LAKR-KRTSRSFKGKDDFIEEPIPKTAIQYLWRRFE-APEARLAKIILTPFGGKMSEIAD 428

Query: 428 TETPSPYRAGYPIKIFYYVLW-EQPDAD----ARHLGWTRELYNYMTPFVSKSPRAVYAN 487
            E P P+R G   +I Y   W E+ D +     ++L W   +Y +MTP+VSKSPR  Y N
Sbjct: 429 NEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVN 488

Query: 488 CRDLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIP 535
            RD+D+G      + T YEEA VWG+KYF NNF++LV VKT VDP++FF  EQSIP
Sbjct: 489 FRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MS025174 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 485.7 bits (1249), Expect = 4.9e-137
Identity = 257/536 (47.95%), Postives = 350/536 (65.30%), Query Frame = 0

Query: 7   YSSFISLLLLAYAILSS--SSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYSSILN 66
           +SSF+  +LL    +S   S+ G+N EDFL C+S   + N     +IHT    S+ SIL+
Sbjct: 3   FSSFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILD 62

Query: 67  FNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYVS-S 126
            +I+NPRF  S+T KPV+IITP +AS VQ  + C + HG  +RTRS GH +EGLSY++ +
Sbjct: 63  SSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYN 122

Query: 127 VPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGF 186
            PF +IDL NL S+++D  + T WVQ+GAT GELYY IG+ TK+L FP  + P VGVGG 
Sbjct: 123 KPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQ 182

Query: 187 LSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLAW 246
            SGGG+G ++RKYGLAADN++DA  VDA+G++ DR++MG+D FWAIRGGGG SFG++L+W
Sbjct: 183 FSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSW 242

Query: 247 KLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGGGRM 306
           K+KLV VP+ +T F + KT  +   +++ +WQY A +  ++LFI  T+   N +      
Sbjct: 243 KVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKN-----A 302

Query: 307 MRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGELSEV 366
           + ALF  L++G    LL +ME+ FPELGL+KE C E SWIES  +L  A  F  GE   V
Sbjct: 303 VHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIES--VLWFAD-FPKGESLGV 362

Query: 367 LVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEISE 426
           L  +    S  +K K DF  EPI E A++ IW R +        K+IL P GGKM+E++E
Sbjct: 363 LTNR-ERTSLSFKGKDDFVQEPIPEAAIQEIWRRLE-APEARLGKIILTPFGGKMSEMAE 422

Query: 427 TETPSPYRAGYPIKIFYYVLW-EQPDADA----RHLGWTRELYNYMTPFVSKSPRAVYAN 486
            ETP P+R G   +I Y   W E+ D +     ++L W   +Y +MTP+VSKSPR  Y N
Sbjct: 423 YETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVN 482

Query: 487 CRDLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIP 535
            +D+D+G    +   T YEE   WG+KYF NNFE+LV VKT+VDP +FF  EQSIP
Sbjct: 483 FKDMDLGMYLGKK-KTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of MS025174 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 479.6 bits (1233), Expect = 3.5e-135
Identity = 255/540 (47.22%), Postives = 349/540 (64.63%), Query Frame = 0

Query: 1   MNFVGSYSSFISLLLLAYAILS----SSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSS 60
           M F   +SS +     +  +LS     S++ +N   FL C+SL  + ++  S +IHTP+ 
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPND 60

Query: 61  PSYSSILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFE 120
            S+SS+L  +I+N RF   D  KPV I+TP Q S VQ++V C +  G  IRTRSGGHD+E
Sbjct: 61  TSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYE 120

Query: 121 GLSYVSSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVP 180
           GLSYV+  PFV++DL NL S+TVD  + + WVQ+GATIGELYY IG+K +TL FP  V P
Sbjct: 121 GLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCP 180

Query: 181 NVGVGGFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGS 240
            VGVGG  SGGG+G ++RK+GLAAD+V+DA  VDA G++ +R+ MG+D FWAIRGGGG S
Sbjct: 181 TVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSS 240

Query: 241 FGIVLAWKLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNS 300
           F +VL+WK+ L+ VP+ VT F + K  +Q+  K++H+WQ+VA +  ++LFI V +    +
Sbjct: 241 FCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKN 300

Query: 301 SEGGGRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFV 360
                 M+RA F  L+LG  + LL ++ K FPELGL+++DC E SWIES     I    +
Sbjct: 301 ------MVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESV----IWFAEL 360

Query: 361 SGELSEVLVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGG 420
             E   VL K+    S  +K KSDF  EP+ +TA+  +W R +     E  +LI  P GG
Sbjct: 361 GEEPINVLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQ-EPEAEHAQLIFTPFGG 420

Query: 421 KMNEISETETPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVY 480
           KM+EI++ ETP P+R G   +I Y   W + D   +++ W   +Y+ M+ FV+KSPR  Y
Sbjct: 421 KMSEIADYETPFPHRKGNIYEIQYLNYW-RGDVKEKYMRWVERVYDDMSEFVAKSPRGAY 480

Query: 481 ANCRDLDIGRNNEEGISTS-YEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIPP 536
            N RDLD+G     G+  S YEE   WG+KYF NNFE+LV VKT VDP +FF  EQSIPP
Sbjct: 481 INLRDLDLGM--YVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of MS025174 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 477.2 bits (1227), Expect = 1.7e-134
Identity = 248/528 (46.97%), Postives = 332/528 (62.88%), Query Frame = 0

Query: 9   SFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYSSILNFNIR 68
           + I +L     I  SSS   N E F  C++ +S      S  I    + SYSS+L  NIR
Sbjct: 4   ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIR 63

Query: 69  NPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSYV--SSVPF 128
           N RF  + T KP  II  +  SHVQA++ C K H  Q++ RSGGHD++GLSYV  S  PF
Sbjct: 64  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 123

Query: 129 VLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSG 188
            ++D+ NL SV VD    TAWVQ+GA +GE+YY I EK+KTL +P  + P VGVGG +SG
Sbjct: 124 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 183

Query: 189 GGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIVLAWKLK 248
           GG+G M+RKYGL  DN +DA  VD NGK+ DRK MG+DL+WAI GGGGGS+G+VLA+K+ 
Sbjct: 184 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 243

Query: 249 LVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTARNSSEGGGRMMRA 308
           LV VP  VT F I++T +QN   ++H+WQ VAP+  + LFI   +   N +    + +R 
Sbjct: 244 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRT 303

Query: 309 LFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGELSEVLVK 368
            F ++FLG    LL I+ + FPELGL + DC ETSWI+S  +L   +  V    + +L +
Sbjct: 304 TFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQS--VLFWTNIQVGSSETLLLQR 363

Query: 369 KPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPHGGKMNEISETET 428
             P+N    K KSD+  EPI  T +E IW++   +  +E   +  NP+GG+M  IS T T
Sbjct: 364 NQPVN--YLKRKSDYVREPISRTGLESIWKK---MIELEIPTMAFNPYGGEMGRISSTVT 423

Query: 429 PSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCRDLDIGR 488
           P PYRAG   KI Y   W       R++  TR+LY +MTPFVSK+PR  + N RD+D+G 
Sbjct: 424 PFPYRAGNLWKIQYGANWRDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGI 483

Query: 489 NNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQSIP 535
           N+  G  +SY E   +G KYF  NFE+LV++KT+VD  NFF +EQSIP
Sbjct: 484 NSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of MS025174 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 471.5 bits (1212), Expect = 9.5e-133
Identity = 250/543 (46.04%), Postives = 345/543 (63.54%), Query Frame = 0

Query: 1   MNFVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSN-SILIHTPSSPSY 60
           M  + +++  + L+ L   + S SS   + + FL C+S HS ++  N    IH P S  Y
Sbjct: 1   MRTLEAFALSLFLVFLVKWVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVY 60

Query: 61  SSILNFNI-RNPRF--WGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFE 120
           +      I +N RF      + KP+ I+TP   + +Q S++C +  G ++RT+SGGHD+E
Sbjct: 61  TDFSQSLISQNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYE 120

Query: 121 GLSYVS-SVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVV 180
           GLSY+S   PF+++DL+N+ S+ ++  D TAWV +GATIGELYY+I + +K  GFP    
Sbjct: 121 GLSYLSLHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTC 180

Query: 181 PNVGVGGFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHD-RKSMGDDLFWAIRGGGG 240
           P+VGVGG  SGGGFG M+RK+GLAADNVVDA FVDANG++++ R+ MG+DLFWAIRGGG 
Sbjct: 181 PSVGVGGHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGA 240

Query: 241 GSFGIVLAWKLKLVPVPAIVTCFAINKTWDQNVAKLVHQWQYVAPEADENLFISVTVTAR 300
            SFG+VL+WK+KLV VP  VTCF  N    QN+ K+VH+WQ +A E D+NLFI V V+  
Sbjct: 241 ASFGVVLSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSI- 300

Query: 301 NSSEGGGRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASG 360
                 G  ++  F + +LG  ++L+ +M + FPELGL  +DC E +WI+S    +   G
Sbjct: 301 -----SGGSVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYFNWKKG 360

Query: 361 FVSGELSEVLVKKPPLNSRRYKVKSDFATEPIRETAMEGIWERFKIIGGIETVKLILNPH 420
                L  +L +    N   +K KSDF   PI E  +EGIW RF     +E+  +I+ P 
Sbjct: 361 ---QPLETLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRFH---EVESPIMIMEPL 420

Query: 421 GGKMNEISETETPSPYRAGYPIKIFYYVLWEQPDADA--RHLGWTRELYNYMTPFVSKSP 480
           GGKM EI ETETP P+R G    I Y V W   D     +H+ W R LY YM  +VS SP
Sbjct: 421 GGKMYEIGETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASP 480

Query: 481 RAVYANCRDLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLNFFFHEQS 536
           R  Y N RDLD+G N   G++TS+E+A +WG +YFG+NF++L  VK K+DP NFF +EQS
Sbjct: 481 RGAYLNYRDLDLGMN--RGVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQS 529