MS025173 (gene) Bitter gourd (TR) v1

BLAST of MS025173 vs. NCBI nr
Match: XP_022152844.1 (berberine bridge enzyme-like 27 [Momordica charantia])

HSP 1 Score: 902.5 bits (2331), Expect = 1.9e-258
Identity = 450/525 (85.71%), Postives = 476/525 (90.67%), Query Frame = 0

Query: 1   MNFVGRYFSFIS-LLLAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYS 60
           MNFVG Y SFIS LLLA+AIL SSSDGNNLEDFLHCISLHSSTNDSN ILIHTPSSPSYS
Sbjct: 1   MNFVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYS 60

Query: 61  SILNFNIGNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSY 120
           SILNFNI N RF G DT KPVAIITPS+ASHVQASVICC+NH  QIRTRSGGHD EGLSY
Sbjct: 61  SILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSY 120

Query: 121 VSNVPFVLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV 180
           VS+VPFVL+DL+NLNSVTVDT++ST WVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV
Sbjct: 121 VSSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV 180

Query: 181 GGFLSGGGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIV 240
           GGFLSGGG G MVRKYGLAADNVVDAY VDANGKLHDRKSMG+DLFWAIRGGGGGSFGIV
Sbjct: 181 GGFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIV 240

Query: 241 LAWKLKLVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVG 300
           LAWKLKLVPVP IVT  AI+KTWDQ+VAKLVH+WQYVAPEADENLFISV VTA NSSE G
Sbjct: 241 LAWKLKLVPVPAIVTCIAINKTWDQSVAKLVHQWQYVAPEADENLFISVTVTARNSSEGG 300

Query: 301 GRMMRALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEH 360
           GRMMRALFFSLFLGK EEL+ IMEKSFPEL LKKEDCIETSWIESTAIL +ASGFVSGE 
Sbjct: 301 GRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGEL 360

Query: 361 LEVLVEKFPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNE 420
            EVLV+K PLN+RRYK KSDFATEPI ETAMEGIWERFK  GGI TV+LILNP+GGK+NE
Sbjct: 361 SEVLVKKPPLNSRRYKVKSDFATEPIPETAMEGIWERFKIIGGIETVKLILNPHGGKMNE 420

Query: 421 ISETETPSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCR 480
           ISET+TPSP+RAGYPIKIFYYV WEQPDADARHL W+RELY+YMTPFVSKSPRAVY NCR
Sbjct: 421 ISETKTPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCR 480

Query: 481 DLDIGRNNEDGIRTSYEEASVWGLKYFGNNFKSGPTFDSSARPCN 525
           DLDIGRNNE+GI TSYEEASVWGLKYFGNNF+      +   P N
Sbjct: 481 DLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLN 525

BLAST of MS025173 vs. NCBI nr
Match: XP_038895067.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 745.0 bits (1922), Expect = 5.1e-211
Identity = 364/509 (71.51%), Postives = 423/509 (83.10%), Query Frame = 0

Query: 3   FVGRYFSFISLLLAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSIL 62
           F   Y+ FI LLL F++      G N+EDF+HCI LHS+ NDSN  LIHTPSS SYS +L
Sbjct: 4   FCSCYYGFIFLLL-FSLATLGCWGYNMEDFVHCIYLHSNNNDSNSTLIHTPSSSSYSYVL 63

Query: 63  NFNIGNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVSN 122
           NF+I NLRFS  +TPKPVAIITPS AS VQA+VICC++H  QIRTRSGGHD EG SYV+N
Sbjct: 64  NFSIRNLRFSEPETPKPVAIITPSHASQVQAAVICCKSHGFQIRTRSGGHDFEGSSYVAN 123

Query: 123 VPFVLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGF 182
           VPFVL+DL+NLNS+T+DTE+ + WVQSGAT+GELYYRIGEK++TLGFP   VP +G+GGF
Sbjct: 124 VPFVLIDLINLNSITIDTEDESAWVQSGATVGELYYRIGEKSRTLGFPAGFVPTIGIGGF 183

Query: 183 LSGGGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAW 242
           LSGGG G MVRKYGLAADNVVDAY+VDANG + DR SMGEDLFWAIRGGGGGSFGIVLAW
Sbjct: 184 LSGGGFGMMVRKYGLAADNVVDAYVVDANGSVFDRYSMGEDLFWAIRGGGGGSFGIVLAW 243

Query: 243 KLKLVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGRM 302
           KLKLVPVP  VTSF++SKTWDQN A L+HRWQYVAPE D++LFIS  VTA+NSS+  GR+
Sbjct: 244 KLKLVPVPPTVTSFSLSKTWDQNAADLIHRWQYVAPEVDQDLFISAWVTAFNSSDGSGRI 303

Query: 303 MRALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLEV 362
           M A FFSL+LGK  EL+S+MEK+FPEL LKKEDC+E SW+ES A    ASGFVS + LE+
Sbjct: 304 MEASFFSLYLGKATELLSLMEKTFPELGLKKEDCLEMSWVESMAF--SASGFVSAKSLEL 363

Query: 363 LVEKFPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEISE 422
           L+ + PL+N R K KSD+ TEPISETA+EG+WERFK+   +  VQ IL P+GGK+NEISE
Sbjct: 364 LLNRSPLDNGRQKIKSDYPTEPISETALEGMWERFKNE-ELEKVQFILIPFGGKMNEISE 423

Query: 423 TETPSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCRDLD 482
           +ETPS HRAGYPI I YYV W+ PDAD+RHLEW+RELY+YMTPFVSKSPRA Y N RDLD
Sbjct: 424 SETPSAHRAGYPIHIGYYVKWQHPDADSRHLEWTRELYNYMTPFVSKSPRAAYANYRDLD 483

Query: 483 IGRNNEDGIRTSYEEASVWGLKYFGNNFK 512
           IG NNED I TSYEEASVWG +YFGNNF+
Sbjct: 484 IGTNNEDRILTSYEEASVWGHQYFGNNFE 508

BLAST of MS025173 vs. NCBI nr
Match: XP_038894011.1 (berberine bridge enzyme-like 18 isoform X2 [Benincasa hispida])

HSP 1 Score: 740.0 bits (1909), Expect = 1.7e-209
Identity = 360/510 (70.59%), Postives = 426/510 (83.53%), Query Frame = 0

Query: 3   FVGRYFSFISLLLAFAILGSSSDGNNLEDFLHCISLHSS-TNDSNPILIHTPSSPSYSSI 62
           F   Y+SFISLLL  ++      G+N EDF+HCISLHSS  NDSN  LIHTPSS SYS +
Sbjct: 4   FWSSYYSFISLLLLLSLATLGCWGDNGEDFVHCISLHSSNNNDSNTTLIHTPSSSSYSYV 63

Query: 63  LNFNIGNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVS 122
           LNF+I NLRFS  +TPKPVAIITPS ASHVQA+VICC++H LQIRTRSGGHD EG SYV+
Sbjct: 64  LNFSIRNLRFSEPETPKPVAIITPSHASHVQAAVICCKSHGLQIRTRSGGHDFEGRSYVA 123

Query: 123 NVPFVLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGG 182
           NVPFVL+DL+NLNS+T+DTE+ + WVQSGATIG+LYYRI EK++TLGFP    P +GVGG
Sbjct: 124 NVPFVLIDLINLNSITIDTEDESAWVQSGATIGKLYYRIAEKSQTLGFPAGFAPTIGVGG 183

Query: 183 FLSGGGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLA 242
           +LSGGG G MVRKYGLA DNVVDAY+VDANG++ DR SMGEDLFWAIRGGGGGSFGIVLA
Sbjct: 184 YLSGGGFGMMVRKYGLAVDNVVDAYVVDANGRIFDRHSMGEDLFWAIRGGGGGSFGIVLA 243

Query: 243 WKLKLVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGR 302
           WKLKLVPVP IVTSFA+ KTWDQN A L+HRWQYVAPE DE+LFI+  VTA NSS+ GGR
Sbjct: 244 WKLKLVPVPPIVTSFALHKTWDQNAADLIHRWQYVAPEVDEDLFITAWVTASNSSKEGGR 303

Query: 303 MMRALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLE 362
           +M A FFSLFLGK ++L+S+M+K+FPEL LKKEDC+E SWIES A   +ASGFVS  +++
Sbjct: 304 IMEASFFSLFLGKADKLLSLMQKTFPELGLKKEDCLEMSWIESMAF--MASGFVSANNVK 363

Query: 363 VLVEKFPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEIS 422
           +L+++ PL+N RYK KSD+AT+PIS+TA+EG+WERFK    + TVQL+L P+GGK+NEIS
Sbjct: 364 LLLDRTPLSNDRYKTKSDYATKPISKTALEGMWERFK-IEELETVQLMLIPFGGKMNEIS 423

Query: 423 ETETPSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCRDL 482
           ETETP  +RAGYPI+I YYV W+ PDAD RHL+W++EL+ YMTPFVSKSPR  Y N +DL
Sbjct: 424 ETETPFAYRAGYPIQIGYYVTWQDPDADLRHLKWTQELHDYMTPFVSKSPRGAYINYKDL 483

Query: 483 DIGRNNEDGIRTSYEEASVWGLKYFGNNFK 512
           DIG N EDGI TSYEEA VWG  YFGNNF+
Sbjct: 484 DIGTNKEDGIPTSYEEAGVWGHPYFGNNFE 510

BLAST of MS025173 vs. NCBI nr
Match: XP_038895033.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 737.3 bits (1902), Expect = 1.1e-208
Identity = 359/509 (70.53%), Postives = 425/509 (83.50%), Query Frame = 0

Query: 3   FVGRYFSFISLLLAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSIL 62
           F   ++ FISLLL F++      G N+EDF+HCI+LHS+ NDSN  LIH PSS SYS IL
Sbjct: 4   FSSCFYGFISLLLLFSLGTFGCWGYNMEDFVHCINLHSNNNDSNSTLIHLPSSSSYSYIL 63

Query: 63  NFNIGNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVSN 122
           NF+I N RFS  +TPKPVAIITPS AS VQA+VICC++H  QIRTRSGGHD EG SYV+N
Sbjct: 64  NFSICNRRFSDPETPKPVAIITPSHASQVQATVICCKSHGFQIRTRSGGHDFEGRSYVAN 123

Query: 123 VPFVLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGF 182
           VPFVL+DL+NLNS+T++ E+ + WVQSGAT+GELYYRIGEK++TLGFP   VP +GVGGF
Sbjct: 124 VPFVLIDLINLNSITINMEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFVPAIGVGGF 183

Query: 183 LSGGGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAW 242
           LSGGG+G MVRKYGLAADNVVDAY+VD NG++ +R SMGEDLFWAIRGGGGGSFGIVLAW
Sbjct: 184 LSGGGLGMMVRKYGLAADNVVDAYVVDENGRVFNRHSMGEDLFWAIRGGGGGSFGIVLAW 243

Query: 243 KLKLVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGRM 302
           KLKLVPVP IVTSFA+ KTWDQN A L+HRWQYVAPE D++LFI+  VTA NSS+  GR+
Sbjct: 244 KLKLVPVPPIVTSFALHKTWDQNTAHLIHRWQYVAPEVDQDLFITAWVTASNSSDGSGRI 303

Query: 303 MRALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLEV 362
           M A FFSL+LGK  EL+S+ME++FPEL LKKEDC+E SW+ES A    ASGFVS + LE+
Sbjct: 304 MEASFFSLYLGKATELLSLMERTFPELGLKKEDCLEMSWVESMAF--SASGFVSAKSLEL 363

Query: 363 LVEKFPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEISE 422
           L+ + PLNN RYK KSD+ TEPISETA+EG+WERFK+   +  VQL+L P+GGK+NEISE
Sbjct: 364 LLNRTPLNNGRYKIKSDYLTEPISETALEGMWERFKNE-ELEKVQLVLIPFGGKMNEISE 423

Query: 423 TETPSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCRDLD 482
           +ETP PHRA YPI+I Y V W++PDAD+RHLEW+RELYSYMTPFVSKSPRAVY N  DLD
Sbjct: 424 SETPFPHRARYPIQIGYCVTWQRPDADSRHLEWTRELYSYMTPFVSKSPRAVYINNIDLD 483

Query: 483 IGRNNEDGIRTSYEEASVWGLKYFGNNFK 512
           +G NN+DGI TSYEEASVWG +YFGNNF+
Sbjct: 484 LGTNNKDGIPTSYEEASVWGHQYFGNNFE 509

BLAST of MS025173 vs. NCBI nr
Match: XP_038894010.1 (berberine bridge enzyme-like 18 isoform X1 [Benincasa hispida])

HSP 1 Score: 735.3 bits (1897), Expect = 4.1e-208
Identity = 360/511 (70.45%), Postives = 426/511 (83.37%), Query Frame = 0

Query: 3   FVGRYFSFISLLLAFAILGSSSDGNNLEDFLHCISLHSS-TNDSNPILIHTPSSPSYSSI 62
           F   Y+SFISLLL  ++      G+N EDF+HCISLHSS  NDSN  LIHTPSS SYS +
Sbjct: 4   FWSSYYSFISLLLLLSLATLGCWGDNGEDFVHCISLHSSNNNDSNTTLIHTPSSSSYSYV 63

Query: 63  LNFNIGNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVS 122
           LNF+I NLRFS  +TPKPVAIITPS ASHVQA+VICC++H LQIRTRSGGHD EG SYV+
Sbjct: 64  LNFSIRNLRFSEPETPKPVAIITPSHASHVQAAVICCKSHGLQIRTRSGGHDFEGRSYVA 123

Query: 123 NVPFVLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGG 182
           NVPFVL+DL+NLNS+T+DTE+ + WVQSGATIG+LYYRI EK++TLGFP    P +GVGG
Sbjct: 124 NVPFVLIDLINLNSITIDTEDESAWVQSGATIGKLYYRIAEKSQTLGFPAGFAPTIGVGG 183

Query: 183 FLSGGGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLA 242
           +LSGGG G MVRKYGLA DNVVDAY+VDANG++ DR SMGEDLFWAIRGGGGGSFGIVLA
Sbjct: 184 YLSGGGFGMMVRKYGLAVDNVVDAYVVDANGRIFDRHSMGEDLFWAIRGGGGGSFGIVLA 243

Query: 243 WKLKLVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVT-AWNSSEVGG 302
           WKLKLVPVP IVTSFA+ KTWDQN A L+HRWQYVAPE DE+LFI+  VT A NSS+ GG
Sbjct: 244 WKLKLVPVPPIVTSFALHKTWDQNAADLIHRWQYVAPEVDEDLFITAWVTAASNSSKEGG 303

Query: 303 RMMRALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHL 362
           R+M A FFSLFLGK ++L+S+M+K+FPEL LKKEDC+E SWIES A   +ASGFVS  ++
Sbjct: 304 RIMEASFFSLFLGKADKLLSLMQKTFPELGLKKEDCLEMSWIESMAF--MASGFVSANNV 363

Query: 363 EVLVEKFPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEI 422
           ++L+++ PL+N RYK KSD+AT+PIS+TA+EG+WERFK    + TVQL+L P+GGK+NEI
Sbjct: 364 KLLLDRTPLSNDRYKTKSDYATKPISKTALEGMWERFK-IEELETVQLMLIPFGGKMNEI 423

Query: 423 SETETPSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCRD 482
           SETETP  +RAGYPI+I YYV W+ PDAD RHL+W++EL+ YMTPFVSKSPR  Y N +D
Sbjct: 424 SETETPFAYRAGYPIQIGYYVTWQDPDADLRHLKWTQELHDYMTPFVSKSPRGAYINYKD 483

Query: 483 LDIGRNNEDGIRTSYEEASVWGLKYFGNNFK 512
           LDIG N EDGI TSYEEA VWG  YFGNNF+
Sbjct: 484 LDIGTNKEDGIPTSYEEAGVWGHPYFGNNFE 511

BLAST of MS025173 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 5.7e-128
Identity = 238/510 (46.67%), Postives = 336/510 (65.88%), Query Frame = 0

Query: 7   YFSFISLLLAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSILNFNI 66
           YF  ++L L+      S+   N E+FL C+S   + +DS   +IHT   PSY SILN +I
Sbjct: 13  YFLLLNLSLSH-FPSISAQRTNHENFLKCLSHRINEDDSR--IIHTSKDPSYFSILNSSI 72

Query: 67  GNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVS-NVPF 126
            N RF   +TPKPV+IITP +A+ VQ+++ C R H + IRTRSGGHD EGLSY++ + PF
Sbjct: 73  QNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPF 132

Query: 127 VLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSG 186
           V++DL NL S+T+D +N T WVQSGATIGELYY IG+ +K+L FP  + P VG+GG   G
Sbjct: 133 VVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGG 192

Query: 187 GGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAWKLK 246
           GG G ++RKYGL+ADNV+DA++VDANG   DR+ MGED FWAIRGGGG SF +VL+WK++
Sbjct: 193 GGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIR 252

Query: 247 LVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGRMMRA 306
           L+ VP +VT F + KT ++    ++++WQY+A +   +LFI  ++     +EV      A
Sbjct: 253 LLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ--KETEV-----YA 312

Query: 307 LFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLEVLVE 366
            F  L+LG   +L+++M+  FPEL L+  +C E SWIES         F+ GE +E+L +
Sbjct: 313 SFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL------WFIKGESMEILAK 372

Query: 367 KFPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEISETET 426
           +    +R +K K DF  EPI +TA++ +W RF++       ++IL P+GGK++EI++ E 
Sbjct: 373 R-KRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEA-RLAKIILTPFGGKMSEIADNEI 432

Query: 427 PSPHRAGYPIKIFYYVAW-EQPDAD----ARHLEWSRELYSYMTPFVSKSPRAVYTNCRD 486
           P PHR G   +I Y   W E+ D +     ++L W   +Y +MTP+VSKSPR  Y N RD
Sbjct: 433 PFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRD 492

Query: 487 LDIGRNNEDGIRTSYEEASVWGLKYFGNNF 511
           +D+G      ++T YEEA VWG+KYF NNF
Sbjct: 493 IDLGMYLGLNMKTKYEEAKVWGVKYFKNNF 504

BLAST of MS025173 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.8e-127
Identity = 230/508 (45.28%), Postives = 324/508 (63.78%), Query Frame = 0

Query: 8   FSFISLLLAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSILNFNIG 67
           F F+S  +  +I       N  E+FL C S H   N +NP L++T     Y SILN  I 
Sbjct: 16  FFFLSFHIQISI------ANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQ 75

Query: 68  NLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVSNVPFVL 127
           NLRF    TPKP+ I+TPS  SH+QA+++C +   LQIRTRSGGHD+EG+SY+S VPFV+
Sbjct: 76  NLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVV 135

Query: 128 VDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSGGG 187
           VDL N++S+ +D  + T WV++GAT+GE+YY I EK + L FPG   P VGVGG  SGGG
Sbjct: 136 VDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGG 195

Query: 188 IGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLV 247
            GA++R YGLAADN++DA+LV+ +GK+ DRKSMGEDLFWAIRGGGG +FGI+ AWK+KLV
Sbjct: 196 YGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLV 255

Query: 248 PVPLIVTSFAISKTWD-QNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGR---MM 307
            VP   T F++ K  +   + KL ++WQ +A + D++L +       N ++  G+    +
Sbjct: 256 AVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTV 315

Query: 308 RALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGF-VSGEHLEV 367
              F S+F G  + L+ +M KSFPEL +KK DC E SWI++T        F  +    E+
Sbjct: 316 HGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEI 375

Query: 368 LVEKFPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEISE 427
           L+++       +  K D+  +PI ETAM  I E+      +G    +L PYGG + EISE
Sbjct: 376 LLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEE-DVGAGMYVLYPYGGIMEEISE 435

Query: 428 TETPSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCRDLD 487
           +  P PHRAG   +++Y  +WE+ + + +H+ W R +Y++ TP+VS++PR  Y N RDLD
Sbjct: 436 SAIPFPHRAGIMYELWYTASWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLD 495

Query: 488 IGRNNEDGIRTSYEEASVWGLKYFGNNF 511
           +G+ N      +Y +A +WG KYFG NF
Sbjct: 496 LGKTNH-ASPNNYTQARIWGEKYFGKNF 515

BLAST of MS025173 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 3.7e-127
Identity = 239/515 (46.41%), Postives = 334/515 (64.85%), Query Frame = 0

Query: 7   YFSFISLLLAFAILGS---SSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSILN 66
           + SF+  +L F++  S   S+ G+N EDFL C+S   + N   P +IHT    S+ SIL+
Sbjct: 3   FSSFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILD 62

Query: 67  FNIGNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVS-N 126
            +I N RFS  +TPKPV+IITP KAS VQ  + C + H + +RTRS GH  EGLSY++ N
Sbjct: 63  SSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYN 122

Query: 127 VPFVLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGF 186
            PF ++DL NL S+++D +N T WVQ+GAT GELYY IG+ TK+L FP  + P VGVGG 
Sbjct: 123 KPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQ 182

Query: 187 LSGGGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAW 246
            SGGG G ++RKYGLAADN++DA +VDA+G++ DR++MGED FWAIRGGGG SFG++L+W
Sbjct: 183 FSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSW 242

Query: 247 KLKLVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGRM 306
           K+KLV VP  +T F + KT  +   +++ +WQY A +  ++LFI   +   N + V    
Sbjct: 243 KVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKNAV---- 302

Query: 307 MRALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLEV 366
             ALF  L++G    L+++ME+ FPEL L+KE C E SWIES       + F  GE L V
Sbjct: 303 -HALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWF---ADFPKGESLGV 362

Query: 367 LVEKFPLNNRRYKAKSDFATEPISETAMEGIWERFKS-TGGIGTVQLILNPYGGKLNEIS 426
           L  +    +  +K K DF  EPI E A++ IW R ++    +G  ++IL P+GGK++E++
Sbjct: 363 LTNR-ERTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLG--KIILTPFGGKMSEMA 422

Query: 427 ETETPSPHRAGYPIKIFYYVAW-EQPDADA----RHLEWSRELYSYMTPFVSKSPRAVYT 486
           E ETP PHR G   +I Y   W E+ D +     ++L+W   +Y +MTP+VSKSPR  Y 
Sbjct: 423 EYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYV 482

Query: 487 NCRDLDIGRNNEDGIRTSYEEASVWGLKYFGNNFK 512
           N +D+D+G       +T YEE   WG+KYF NNF+
Sbjct: 483 NFKDMDLGMYLGKK-KTKYEEGKSWGVKYFKNNFE 505

BLAST of MS025173 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 3.1e-126
Identity = 231/523 (44.17%), Postives = 330/523 (63.10%), Query Frame = 0

Query: 8   FSFISLLLAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSILNFNIG 67
           F F+S  +  +I       N  E+FL C S +   N +NP  I+T     Y S+LN  I 
Sbjct: 16  FFFLSFNIQISI------ANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQ 75

Query: 68  NLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVSNVPFVL 127
           NLRF+   TPKP+ I+TPS  SH+QAS++C +   LQIRTRSGGHD+EGLSY+S VPF +
Sbjct: 76  NLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAI 135

Query: 128 VDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSGGG 187
           VDL N+++V VD  + T WV++GAT+GE+YY I E  +   FPG   P VGVGG  SGGG
Sbjct: 136 VDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGG 195

Query: 188 IGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLV 247
            GA++R YGLAADN++DA+LV+ +GK+ DRKSMGEDLFWAIRGGGG +FGI+ AWK+KLV
Sbjct: 196 YGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLV 255

Query: 248 PVPLIVTSFAISKTWD-QNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGR---MM 307
            VP   T F++ K  +   + KL ++WQ +A + D++L ++      N ++  G+    +
Sbjct: 256 VVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTV 315

Query: 308 RALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGF-VSGEHLEV 367
              F S+FLG  + L+ +M KSFPEL +KK DC E SWI++T        +  +    E+
Sbjct: 316 HGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEI 375

Query: 368 LVEKFPLNNRRYKAKSDFATEPISETAMEGIWER-FKSTGGIGTVQLILNPYGGKLNEIS 427
           L+++       +  K D+  + I ETAM  I E+ ++   G+G    +L PYGG ++EIS
Sbjct: 376 LLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVG--MYVLYPYGGIMDEIS 435

Query: 428 ETETPSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCRDL 487
           E+  P PHRAG   +++Y   WE+ + + +H+ W R +Y++ TP+VS++PR  Y N RDL
Sbjct: 436 ESAIPFPHRAGIMYELWYTATWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDL 495

Query: 488 DIGRNNEDGIRTSYEEASVWGLKYFGNNFKSGPTFDSSARPCN 525
           D+G+ N +    +Y +A +WG KYFG NF       + A P N
Sbjct: 496 DLGKTNPES-PNNYTQARIWGEKYFGKNFNRLVKVKTKADPNN 529

BLAST of MS025173 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.2e-125
Identity = 240/518 (46.33%), Postives = 336/518 (64.86%), Query Frame = 0

Query: 6   RYFSFISLLLAFAILGS---------SSDGNNLEDFLHCISLHSSTNDSNPI--LIHTPS 65
           ++ SF S +L F    +         S++ +N   FL C+SL    NDSN +  +IHTP+
Sbjct: 2   KFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSL--QLNDSNIVSKVIHTPN 61

Query: 66  SPSYSSILNFNIGNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDS 125
             S+SS+L  +I N RFS  D PKPV I+TP + S VQ++V C R   + IRTRSGGHD 
Sbjct: 62  DTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDY 121

Query: 126 EGLSYVSNVPFVLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVV 185
           EGLSYV++ PFV++DL NL S+TVD +N + WVQ+GATIGELYY IG+K +TL FP  V 
Sbjct: 122 EGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVC 181

Query: 186 PNVGVGGFLSGGGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGG 245
           P VGVGG  SGGG G ++RK+GLAAD+V+DA +VDA G++ +R+ MGED FWAIRGGGG 
Sbjct: 182 PTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGS 241

Query: 246 SFGIVLAWKLKLVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWN 305
           SF +VL+WK+ L+ VP  VT F ++K  +Q+  K++HRWQ+VA +  ++LFI V++  + 
Sbjct: 242 SFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYK 301

Query: 306 SSEVGGRMMRALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGF 365
           +      M+RA F  L+LG  + L+ ++ K FPEL L+++DC E SWIES     +    
Sbjct: 302 N------MVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESV----IWFAE 361

Query: 366 VSGEHLEVLVEKFPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYG 425
           +  E + VL ++    +  +KAKSDF  EP+ +TA+  +W R +        QLI  P+G
Sbjct: 362 LGEEPINVLTKR-TRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEA-EHAQLIFTPFG 421

Query: 426 GKLNEISETETPSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAV 485
           GK++EI++ ETP PHR G   +I Y   W + D   +++ W   +Y  M+ FV+KSPR  
Sbjct: 422 GKMSEIADYETPFPHRKGNIYEIQYLNYW-RGDVKEKYMRWVERVYDDMSEFVAKSPRGA 481

Query: 486 YTNCRDLDIGRNNEDGI-RTSYEEASVWGLKYFGNNFK 512
           Y N RDLD+G     G+ R+ YEE   WG+KYF NNF+
Sbjct: 482 YINLRDLDLGM--YVGVKRSKYEEGKSWGVKYFKNNFE 502

BLAST of MS025173 vs. ExPASy TrEMBL
Match: A0A6J1DHA5 (berberine bridge enzyme-like 27 OS=Momordica charantia OX=3673 GN=LOC111020467 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 9.3e-259
Identity = 450/525 (85.71%), Postives = 476/525 (90.67%), Query Frame = 0

Query: 1   MNFVGRYFSFIS-LLLAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYS 60
           MNFVG Y SFIS LLLA+AIL SSSDGNNLEDFLHCISLHSSTNDSN ILIHTPSSPSYS
Sbjct: 1   MNFVGSYSSFISLLLLAYAILSSSSDGNNLEDFLHCISLHSSTNDSNSILIHTPSSPSYS 60

Query: 61  SILNFNIGNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSY 120
           SILNFNI N RF G DT KPVAIITPS+ASHVQASVICC+NH  QIRTRSGGHD EGLSY
Sbjct: 61  SILNFNIRNPRFWGSDTHKPVAIITPSQASHVQASVICCKNHGFQIRTRSGGHDFEGLSY 120

Query: 121 VSNVPFVLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV 180
           VS+VPFVL+DL+NLNSVTVDT++ST WVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV
Sbjct: 121 VSSVPFVLIDLINLNSVTVDTKDSTAWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGV 180

Query: 181 GGFLSGGGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIV 240
           GGFLSGGG G MVRKYGLAADNVVDAY VDANGKLHDRKSMG+DLFWAIRGGGGGSFGIV
Sbjct: 181 GGFLSGGGFGPMVRKYGLAADNVVDAYFVDANGKLHDRKSMGDDLFWAIRGGGGGSFGIV 240

Query: 241 LAWKLKLVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVG 300
           LAWKLKLVPVP IVT  AI+KTWDQ+VAKLVH+WQYVAPEADENLFISV VTA NSSE G
Sbjct: 241 LAWKLKLVPVPAIVTCIAINKTWDQSVAKLVHQWQYVAPEADENLFISVTVTARNSSEGG 300

Query: 301 GRMMRALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEH 360
           GRMMRALFFSLFLGK EEL+ IMEKSFPEL LKKEDCIETSWIESTAIL +ASGFVSGE 
Sbjct: 301 GRMMRALFFSLFLGKAEELLYIMEKSFPELGLKKEDCIETSWIESTAILSIASGFVSGEL 360

Query: 361 LEVLVEKFPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNE 420
            EVLV+K PLN+RRYK KSDFATEPI ETAMEGIWERFK  GGI TV+LILNP+GGK+NE
Sbjct: 361 SEVLVKKPPLNSRRYKVKSDFATEPIPETAMEGIWERFKIIGGIETVKLILNPHGGKMNE 420

Query: 421 ISETETPSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCR 480
           ISET+TPSP+RAGYPIKIFYYV WEQPDADARHL W+RELY+YMTPFVSKSPRAVY NCR
Sbjct: 421 ISETKTPSPYRAGYPIKIFYYVLWEQPDADARHLGWTRELYNYMTPFVSKSPRAVYANCR 480

Query: 481 DLDIGRNNEDGIRTSYEEASVWGLKYFGNNFKSGPTFDSSARPCN 525
           DLDIGRNNE+GI TSYEEASVWGLKYFGNNF+      +   P N
Sbjct: 481 DLDIGRNNEEGISTSYEEASVWGLKYFGNNFEKLVEVKTKVDPLN 525

BLAST of MS025173 vs. ExPASy TrEMBL
Match: A0A6J1HYZ7 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469396 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 3.6e-202
Identity = 350/504 (69.44%), Postives = 416/504 (82.54%), Query Frame = 0

Query: 9   SFIS-LLLAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSILNFNIG 68
           +FIS LLL+FA  G  SD  NLEDFLHCISLHSS   SN  LIHTPSS SY  +LNF+I 
Sbjct: 25  NFISLLLLSFASWGFGSD--NLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIR 84

Query: 69  NLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVSNVPFVL 128
           NLRF+ + TPKPVAI+TP +AS VQA+VICC+ H LQIRTRSGGHD EG SYVSN+PFVL
Sbjct: 85  NLRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVL 144

Query: 129 VDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSGGG 188
           +DL+NLNS+T+D ++ T W QSGAT+GELYY+IGEK++TL FP  +  ++G+GGF SGGG
Sbjct: 145 IDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGG 204

Query: 189 IGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLV 248
           +G +VRKYGLA DNVVDAYLVDANGKL DR SMGEDLFWAIRGGGGGSFGIVLAWKLKLV
Sbjct: 205 LGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLV 264

Query: 249 PVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGRMMRALF 308
            VP  VTSFA+ K+WD N AKL+H WQYVA E DE+LFI+  VTA NSS+ GGR+M+A F
Sbjct: 265 RVPPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASF 324

Query: 309 FSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLEVLVEKF 368
           FSLFLGK  EL+S+MEK+FPEL LKKEDC+E SW+E+ A    AS +VS E+LE+L+++ 
Sbjct: 325 FSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAF--SASEYVSPENLELLLDRT 384

Query: 369 PLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEISETETPS 428
            L   RYK KSD+AT+PISETA+ GIWERFK    + TVQLIL P+GGK+NEISET+TP+
Sbjct: 385 SLITGRYKMKSDYATKPISETALNGIWERFK-IEELETVQLILIPFGGKMNEISETDTPN 444

Query: 429 PHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCRDLDIGRNN 488
           PHRA +PI I YYV W +PDAD+RHLEW+REL+ YMTPFVS+SPRA Y N +DLDIG+NN
Sbjct: 445 PHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNN 504

Query: 489 EDGIRTSYEEASVWGLKYFGNNFK 512
           EDGI TSY+EASVWG +YFG NF+
Sbjct: 505 EDGIPTSYDEASVWGYRYFGINFE 523

BLAST of MS025173 vs. ExPASy TrEMBL
Match: A0A6J1EVN7 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111438506 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 1.4e-201
Identity = 356/519 (68.59%), Postives = 419/519 (80.73%), Query Frame = 0

Query: 8   FSFISLL-LAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSILNFNI 67
           FSFISL+ ++FA  G  SD  NL+DFLHCISLHSS ++SN  LIHTPSS SY  +LNF+I
Sbjct: 15  FSFISLVFVSFASWGCGSD--NLQDFLHCISLHSSNHNSNSTLIHTPSSSSYLYVLNFSI 74

Query: 68  GNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVSNVPFV 127
            NLRF+  DTPKPVAI+TP +AS VQ +VICC+ H LQIRTRSGG D EG SYVSNVPFV
Sbjct: 75  RNLRFAESDTPKPVAIVTPWQASEVQTTVICCKTHGLQIRTRSGGQDFEGRSYVSNVPFV 134

Query: 128 LVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSGG 187
           L+DL NLNS+T+DTE+ T W QSGAT+GELYY+IGEK++TL FP  +V N+G+GGF SGG
Sbjct: 135 LIDLTNLNSITIDTEDETAWAQSGATVGELYYKIGEKSRTLAFPAGLVANLGLGGFFSGG 194

Query: 188 GIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKL 247
           G G +VRKYGLA DNVVDA LVDANGKL DR SMGEDLFWAIRGGGGGSFGIVL WKLKL
Sbjct: 195 GFGMLVRKYGLAVDNVVDAQLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLKL 254

Query: 248 VPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGRMMRAL 307
           V VP  VTSFA+ K+WD N AKL+HRWQYVAPEADE+LFI+  V A NSS+ GGR+M+A 
Sbjct: 255 VRVPPTVTSFAVHKSWDLNAAKLIHRWQYVAPEADEDLFITAWVIASNSSDGGGRIMKAS 314

Query: 308 FFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLEVLVEK 367
           FFSLFLG+  EL+S+M K+FPEL L KEDC+E SW+ES A    AS FVS E LE+L+++
Sbjct: 315 FFSLFLGEAAELVSLMAKAFPELELTKEDCLEMSWVESMAF--SASVFVSAESLELLLDR 374

Query: 368 -FPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEISETET 427
              L+N RYK KSD+ATEPISETA+ GIWERFK    + T+QL+L P+GGK+NEISET+T
Sbjct: 375 TTQLSNGRYKIKSDYATEPISETALNGIWERFK-IEELETLQLVLIPFGGKMNEISETDT 434

Query: 428 PSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCRDLDIGR 487
           P+PHRA +PI I YY  W +PDAD+RHLEW+REL+ YMTPFVSKSPRA Y N +DLDIGR
Sbjct: 435 PNPHRARHPIHIGYYATWHRPDADSRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGR 494

Query: 488 NNEDGIRTSYEEASVWGLKYFGNNFKSGPTFDSSARPCN 525
           NNEDGI TSYEEAS+WG +YFG NF+      + A P N
Sbjct: 495 NNEDGIPTSYEEASIWGHQYFGINFERLVAVKTKADPFN 528

BLAST of MS025173 vs. ExPASy TrEMBL
Match: A0A6J1HP64 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111464765 PE=3 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 5.2e-201
Identity = 352/505 (69.70%), Postives = 414/505 (81.98%), Query Frame = 0

Query: 10  FIS-LLLAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSILNFNIGN 69
           FIS LLL+FA     SD  NL+DFLHCISLHSS + SN  LIHTPSS SY  +LNF+I N
Sbjct: 31  FISLLLLSFASWVCGSD--NLQDFLHCISLHSSNHHSNSTLIHTPSSSSYPHVLNFSIRN 90

Query: 70  LRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVSNVPFVLV 129
           LRF+   TPKPVAI+TP + S VQA+VICC+ H LQIRTRSGGHD EG SYVSNVPFVL+
Sbjct: 91  LRFAESHTPKPVAIVTPWQPSEVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNVPFVLI 150

Query: 130 DLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSGGGI 189
           DL+NLNS+T+D +N T W QSGAT+GELYY+IGEK++TL FP     ++G+GGF SGGG+
Sbjct: 151 DLINLNSITIDVDNETAWAQSGATVGELYYKIGEKSRTLAFPAGFTASIGLGGFFSGGGL 210

Query: 190 GAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKLVP 249
           G +VRKYGLA DNVVDAYLVDANGKL DR SMGEDLFWAIRGGGGGSFGIVLAWKLKLV 
Sbjct: 211 GMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVR 270

Query: 250 VPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSS--EVGGRMMRAL 309
           VP  VTSFA+ K+WD N AKL+HRWQYVAP+ DE+LFI+  VTA NSS  E GGR+M+A 
Sbjct: 271 VPPTVTSFAVHKSWDLNAAKLIHRWQYVAPKVDEDLFITAWVTASNSSEGEGGGRVMKAS 330

Query: 310 FFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLEVLVEK 369
           FFSLFLGK  EL+S+M+K+FPEL LKKEDC+E SW+E+ A    AS +VS E+LE+L+++
Sbjct: 331 FFSLFLGKAAELVSLMKKAFPELELKKEDCLEISWVEAMAF--SASEYVSPENLELLLDR 390

Query: 370 FPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEISETETP 429
            PL   RYK KSD+AT+PISETA+ GIWERFK    + TVQLIL P+GGK+NEISET TP
Sbjct: 391 TPLITGRYKMKSDYATKPISETALNGIWERFK-IEELETVQLILIPFGGKMNEISETATP 450

Query: 430 SPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCRDLDIGRN 489
           +PHRA +PI I YYV W +PDAD+RHLEW+REL+ YMTPFVS+SPRA Y N +DLDIGRN
Sbjct: 451 NPHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGRN 510

Query: 490 NEDGIRTSYEEASVWGLKYFGNNFK 512
           NEDGI TSY+EASVWG +YFG NF+
Sbjct: 511 NEDGIPTSYDEASVWGYRYFGINFE 530

BLAST of MS025173 vs. ExPASy TrEMBL
Match: A0A6J1I038 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469264 PE=3 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 2.0e-200
Identity = 351/519 (67.63%), Postives = 421/519 (81.12%), Query Frame = 0

Query: 8   FSFISLL-LAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSILNFNI 67
           F F+SL+ ++FA  G  SD  NL+DFLHCISLHSS ++SN  LIHTPSS SY  +LNF+I
Sbjct: 15  FGFVSLVFVSFASWGCGSD--NLQDFLHCISLHSSNHNSNSTLIHTPSSSSYLYVLNFSI 74

Query: 68  GNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVSNVPFV 127
            NLRF+  DTPKPVAI+TP +AS VQA+VICC+ H LQIRTRSGG D EG SYVSNVPFV
Sbjct: 75  RNLRFAESDTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGQDFEGRSYVSNVPFV 134

Query: 128 LVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSGG 187
           L+DL+NLNS+T+DTE+ T W QSGAT+GELYY+IG+K++TL FP  +V N+G+GGF SGG
Sbjct: 135 LIDLINLNSITIDTEDETAWAQSGATVGELYYKIGQKSRTLAFPAGLVANLGLGGFFSGG 194

Query: 188 GIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAWKLKL 247
           G G ++RKYGLA DNVVDA LVDANGKL DR SMGEDLFWAIRGGGGGSFGIVL WKLKL
Sbjct: 195 GFGMLLRKYGLAVDNVVDAQLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLKL 254

Query: 248 VPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGRMMRAL 307
           V VP  VTSFA+ K+WD N AKL+H+WQYVAPE DE+LFI+  VTA NSS+ GGR+M+A 
Sbjct: 255 VRVPPTVTSFAVHKSWDLNAAKLIHQWQYVAPEVDEDLFITAWVTASNSSDGGGRIMKAS 314

Query: 308 FFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLEVLVEK 367
           FF+LFLG+  EL+S+M K+FPEL L +EDC+E SW+ES A    AS FVS E+LE+L+++
Sbjct: 315 FFALFLGEAAELVSLMAKAFPELELTREDCLEMSWVESMAF--SASVFVSAENLELLLDR 374

Query: 368 -FPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEISETET 427
              L+N RYK KSD+ATEPISETA+ GIWERFK    + T+QLIL P+GGK+NEISET+T
Sbjct: 375 TTQLSNGRYKIKSDYATEPISETALNGIWERFK-IEELETLQLILIPFGGKMNEISETDT 434

Query: 428 PSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCRDLDIGR 487
           P+PHRA +PI I YY  W +PDAD+RHLEW+REL+ YMTPFVSKSPRA Y N +DLDIGR
Sbjct: 435 PNPHRARHPIHIGYYATWHRPDADSRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGR 494

Query: 488 NNEDGIRTSYEEASVWGLKYFGNNFKSGPTFDSSARPCN 525
           NNEDGI TSYEEAS+WG +YFG NF+      + A P N
Sbjct: 495 NNEDGIPTSYEEASIWGHQYFGINFERLVEVKTKADPFN 528

BLAST of MS025173 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 459.5 bits (1181), Expect = 4.0e-129
Identity = 238/510 (46.67%), Postives = 336/510 (65.88%), Query Frame = 0

Query: 7   YFSFISLLLAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSILNFNI 66
           YF  ++L L+      S+   N E+FL C+S   + +DS   +IHT   PSY SILN +I
Sbjct: 13  YFLLLNLSLSH-FPSISAQRTNHENFLKCLSHRINEDDSR--IIHTSKDPSYFSILNSSI 72

Query: 67  GNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVS-NVPF 126
            N RF   +TPKPV+IITP +A+ VQ+++ C R H + IRTRSGGHD EGLSY++ + PF
Sbjct: 73  QNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPF 132

Query: 127 VLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFLSG 186
           V++DL NL S+T+D +N T WVQSGATIGELYY IG+ +K+L FP  + P VG+GG   G
Sbjct: 133 VVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGG 192

Query: 187 GGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAWKLK 246
           GG G ++RKYGL+ADNV+DA++VDANG   DR+ MGED FWAIRGGGG SF +VL+WK++
Sbjct: 193 GGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIR 252

Query: 247 LVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGRMMRA 306
           L+ VP +VT F + KT ++    ++++WQY+A +   +LFI  ++     +EV      A
Sbjct: 253 LLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ--KETEV-----YA 312

Query: 307 LFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLEVLVE 366
            F  L+LG   +L+++M+  FPEL L+  +C E SWIES         F+ GE +E+L +
Sbjct: 313 SFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL------WFIKGESMEILAK 372

Query: 367 KFPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEISETET 426
           +    +R +K K DF  EPI +TA++ +W RF++       ++IL P+GGK++EI++ E 
Sbjct: 373 R-KRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEA-RLAKIILTPFGGKMSEIADNEI 432

Query: 427 PSPHRAGYPIKIFYYVAW-EQPDAD----ARHLEWSRELYSYMTPFVSKSPRAVYTNCRD 486
           P PHR G   +I Y   W E+ D +     ++L W   +Y +MTP+VSKSPR  Y N RD
Sbjct: 433 PFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRD 492

Query: 487 LDIGRNNEDGIRTSYEEASVWGLKYFGNNF 511
           +D+G      ++T YEEA VWG+KYF NNF
Sbjct: 493 IDLGMYLGLNMKTKYEEAKVWGVKYFKNNF 504

BLAST of MS025173 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 456.8 bits (1174), Expect = 2.6e-128
Identity = 239/515 (46.41%), Postives = 334/515 (64.85%), Query Frame = 0

Query: 7   YFSFISLLLAFAILGS---SSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSILN 66
           + SF+  +L F++  S   S+ G+N EDFL C+S   + N   P +IHT    S+ SIL+
Sbjct: 3   FSSFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILD 62

Query: 67  FNIGNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVS-N 126
            +I N RFS  +TPKPV+IITP KAS VQ  + C + H + +RTRS GH  EGLSY++ N
Sbjct: 63  SSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYN 122

Query: 127 VPFVLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGF 186
            PF ++DL NL S+++D +N T WVQ+GAT GELYY IG+ TK+L FP  + P VGVGG 
Sbjct: 123 KPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQ 182

Query: 187 LSGGGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAW 246
            SGGG G ++RKYGLAADN++DA +VDA+G++ DR++MGED FWAIRGGGG SFG++L+W
Sbjct: 183 FSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSW 242

Query: 247 KLKLVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGRM 306
           K+KLV VP  +T F + KT  +   +++ +WQY A +  ++LFI   +   N + V    
Sbjct: 243 KVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKNAV---- 302

Query: 307 MRALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLEV 366
             ALF  L++G    L+++ME+ FPEL L+KE C E SWIES       + F  GE L V
Sbjct: 303 -HALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWF---ADFPKGESLGV 362

Query: 367 LVEKFPLNNRRYKAKSDFATEPISETAMEGIWERFKS-TGGIGTVQLILNPYGGKLNEIS 426
           L  +    +  +K K DF  EPI E A++ IW R ++    +G  ++IL P+GGK++E++
Sbjct: 363 LTNR-ERTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLG--KIILTPFGGKMSEMA 422

Query: 427 ETETPSPHRAGYPIKIFYYVAW-EQPDADA----RHLEWSRELYSYMTPFVSKSPRAVYT 486
           E ETP PHR G   +I Y   W E+ D +     ++L+W   +Y +MTP+VSKSPR  Y 
Sbjct: 423 EYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYV 482

Query: 487 NCRDLDIGRNNEDGIRTSYEEASVWGLKYFGNNFK 512
           N +D+D+G       +T YEE   WG+KYF NNF+
Sbjct: 483 NFKDMDLGMYLGKK-KTKYEEGKSWGVKYFKNNFE 505

BLAST of MS025173 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 451.8 bits (1161), Expect = 8.4e-127
Identity = 240/518 (46.33%), Postives = 336/518 (64.86%), Query Frame = 0

Query: 6   RYFSFISLLLAFAILGS---------SSDGNNLEDFLHCISLHSSTNDSNPI--LIHTPS 65
           ++ SF S +L F    +         S++ +N   FL C+SL    NDSN +  +IHTP+
Sbjct: 2   KFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSL--QLNDSNIVSKVIHTPN 61

Query: 66  SPSYSSILNFNIGNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDS 125
             S+SS+L  +I N RFS  D PKPV I+TP + S VQ++V C R   + IRTRSGGHD 
Sbjct: 62  DTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDY 121

Query: 126 EGLSYVSNVPFVLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVV 185
           EGLSYV++ PFV++DL NL S+TVD +N + WVQ+GATIGELYY IG+K +TL FP  V 
Sbjct: 122 EGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVC 181

Query: 186 PNVGVGGFLSGGGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGG 245
           P VGVGG  SGGG G ++RK+GLAAD+V+DA +VDA G++ +R+ MGED FWAIRGGGG 
Sbjct: 182 PTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGS 241

Query: 246 SFGIVLAWKLKLVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWN 305
           SF +VL+WK+ L+ VP  VT F ++K  +Q+  K++HRWQ+VA +  ++LFI V++  + 
Sbjct: 242 SFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYK 301

Query: 306 SSEVGGRMMRALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGF 365
           +      M+RA F  L+LG  + L+ ++ K FPEL L+++DC E SWIES     +    
Sbjct: 302 N------MVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESV----IWFAE 361

Query: 366 VSGEHLEVLVEKFPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYG 425
           +  E + VL ++    +  +KAKSDF  EP+ +TA+  +W R +        QLI  P+G
Sbjct: 362 LGEEPINVLTKR-TRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEA-EHAQLIFTPFG 421

Query: 426 GKLNEISETETPSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAV 485
           GK++EI++ ETP PHR G   +I Y   W + D   +++ W   +Y  M+ FV+KSPR  
Sbjct: 422 GKMSEIADYETPFPHRKGNIYEIQYLNYW-RGDVKEKYMRWVERVYDDMSEFVAKSPRGA 481

Query: 486 YTNCRDLDIGRNNEDGI-RTSYEEASVWGLKYFGNNFK 512
           Y N RDLD+G     G+ R+ YEE   WG+KYF NNF+
Sbjct: 482 YINLRDLDLGM--YVGVKRSKYEEGKSWGVKYFKNNFE 502

BLAST of MS025173 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 448.7 bits (1153), Expect = 7.1e-126
Identity = 234/508 (46.06%), Postives = 315/508 (62.01%), Query Frame = 0

Query: 6   RYFSFISLLLAFAILGSSSDGNNLEDFLHCISLHSSTNDSNPILIHTPSSPSYSSILNFN 65
           +Y   + L     I  SSS   N E F  C++ +S         I    + SYSS+L  N
Sbjct: 2   KYALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQAN 61

Query: 66  IGNLRFSGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYV--SNV 125
           I NLRF+   TPKP  II  +  SHVQA++ C + H+LQ++ RSGGHD +GLSYV  S  
Sbjct: 62  IRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGK 121

Query: 126 PFVLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGFL 185
           PF ++D+ NL SV VD  + T WVQ+GA +GE+YY I EK+KTL +P  + P VGVGG +
Sbjct: 122 PFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHI 181

Query: 186 SGGGIGAMVRKYGLAADNVVDAYLVDANGKLHDRKSMGEDLFWAIRGGGGGSFGIVLAWK 245
           SGGG G M+RKYGL  DN +DA +VD NGK+ DRK MGEDL+WAI GGGGGS+G+VLA+K
Sbjct: 182 SGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYK 241

Query: 246 LKLVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGRMM 305
           + LV VP  VT F IS+T +QN   ++HRWQ VAP+  + LFI  ++   N +    + +
Sbjct: 242 INLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTV 301

Query: 306 RALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLEVL 365
           R  F ++FLG T  L+SI+ + FPEL L + DC ETSWI+S  +L   +  V      +L
Sbjct: 302 RTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQS--VLFWTNIQVGSSETLLL 361

Query: 366 VEKFPLNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEISET 425
               P+N    K KSD+  EPIS T +E IW++      +    +  NPYGG++  IS T
Sbjct: 362 QRNQPVN--YLKRKSDYVREPISRTGLESIWKKMIE---LEIPTMAFNPYGGEMGRISST 421

Query: 426 ETPSPHRAGYPIKIFYYVAWEQPDADARHLEWSRELYSYMTPFVSKSPRAVYTNCRDLDI 485
            TP P+RAG   KI Y   W       R++E +R+LY +MTPFVSK+PR  + N RD+D+
Sbjct: 422 VTPFPYRAGNLWKIQYGANWRDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDL 481

Query: 486 GRNNEDGIRTSYEEASVWGLKYFGNNFK 512
           G N+ +G  +SY E   +G KYF  NF+
Sbjct: 482 GINSHNGKISSYVEGKRYGKKYFAGNFE 502

BLAST of MS025173 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 443.0 bits (1138), Expect = 3.9e-124
Identity = 239/513 (46.59%), Postives = 327/513 (63.74%), Query Frame = 0

Query: 11  ISLLLAFAILGSSSDGN---NLEDFLHCISLHSSTNDSNP-ILIHTPSSPSYSSILNFNI 70
           +SL L F +   +SD +   + + FL C+S HS ++  NP   IH P S  Y+      I
Sbjct: 9   LSLFLVFLVKWVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQSLI 68

Query: 71  G-NLRF--SGFDTPKPVAIITPSKASHVQASVICCRNHDLQIRTRSGGHDSEGLSYVS-N 130
             N RF    F + KP+ I+TP   + +Q S++C R   +++RT+SGGHD EGLSY+S +
Sbjct: 69  SQNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYEGLSYLSLH 128

Query: 131 VPFVLVDLVNLNSVTVDTENSTTWVQSGATIGELYYRIGEKTKTLGFPGAVVPNVGVGGF 190
            PF+++DLVN+ S+ ++  + T WV +GATIGELYY+I + +K  GFP    P+VGVGG 
Sbjct: 129 SPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTCPSVGVGGH 188

Query: 191 LSGGGIGAMVRKYGLAADNVVDAYLVDANGKLHD-RKSMGEDLFWAIRGGGGGSFGIVLA 250
            SGGG GAM+RK+GLAADNVVDA  VDANG++++ R+ MGEDLFWAIRGGG  SFG+VL+
Sbjct: 189 FSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGAASFGVVLS 248

Query: 251 WKLKLVPVPLIVTSFAISKTWDQNVAKLVHRWQYVAPEADENLFISVIVTAWNSSEVGGR 310
           WK+KLV VP  VT F  +    QN+ K+VHRWQ +A E D+NLFI VIV+      + G 
Sbjct: 249 WKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVS------ISGG 308

Query: 311 MMRALFFSLFLGKTEELISIMEKSFPELRLKKEDCIETSWIESTAILPLASGFVSGEHLE 370
            ++  F + +LG  ++LI +M + FPEL L  +DC E +WI+S     +   +  G+ LE
Sbjct: 309 SVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSI----MYFNWKKGQPLE 368

Query: 371 VLVEKFP-LNNRRYKAKSDFATEPISETAMEGIWERFKSTGGIGTVQLILNPYGGKLNEI 430
            L+++    N+  +KAKSDF   PI E  +EGIW RF     + +  +I+ P GGK+ EI
Sbjct: 369 TLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRFHE---VESPIMIMEPLGGKMYEI 428

Query: 431 SETETPSPHRAGYPIKIFYYVAWEQPDADA--RHLEWSRELYSYMTPFVSKSPRAVYTNC 490
            ETETP PHR G    I Y V W   D     +H+ W R LY YM  +VS SPR  Y N 
Sbjct: 429 GETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYLNY 488

Query: 491 RDLDIGRNNEDGIRTSYEEASVWGLKYFGNNFK 512
           RDLD+G N   G+ TS+E+A +WG +YFG+NFK
Sbjct: 489 RDLDLGMNR--GVNTSFEDAKLWGFRYFGSNFK 506