MS025058 (gene) Bitter gourd (TR) v1

Overview


Name	MS025058
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Glucan endo-1,3-beta-D-glucosidase
Location	scaffold327: 114574 .. 116149 (+)
RNA-Seq Expression	MS025058
Synteny	MS025058

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACCCTCTAACTCCTCTCCCCTTCCTCATCTTCCTCCTCTGCCTCTCCCCATTTTCAGGTAATTCTTCAAAATCCAAACTCTGCTCTGTTTTTTTCCCAGCAGAATCCTGGGTTTAATCAATAATGCGGTGTTCTTTTTGCTACAGATGGTGGTTCCATTGGAATCAACTATGGCAGAATCGCCAACAACCTCCCTTCCGCCGTTAAGGTCGTTAAGCTCCTCAAATCCCACGGCCTCCACAGGGTCAAAGTTTACGACACCGATCCGGCCGTCCTACGGGCTCTCTCCGGCTCCGGCATTAAGGTCACCGTCGATTTGCCCAACGAGCTCCTCTTCGCCGCCGCCAAACGCCTCACCTTCGCCTACTCCTGGGTCGAGAAGAACGTCGCCGCCTACTACCCTTCCACTGAAATCGAAGCCATTGCCGTCGGAAACGAAGTCTTCGTCGACCCCCACAACACGACGTCGTTTCTTGTTCCGGCCATGAAGAACATTCACCAAGCCCTCGTCAAGTACAACCTCCACTCCGCCATTAAAATCTCTTCCCCCATCGCATTGAGCGCCTTGCAAAACTCCTACCCTTCCTCCGCCGGCTCCTTCCGGCCGGAGCTCGTCGAGACCGTTTTCCGGCCAATGCTGGACTTCCTCCGCCAAACCGGCTCCTACCTCATGGTAAACGCATACCCGTTCTTCGCCTACGAGTCCAACTCCGACGTCATTTCCTTAGACTACGCACTTTTCCGCGACAACCCCGGCGTGGTGGATTCCGGCAGCGGCTACCGGTACTTCAGCCTTTTCGACGCCCAAATCGACGCCGTTTTTGCCGCGATGTCGGCTCTCAAATACGACGACATTAAAATGGTGGTTACGGAGACCGGATGGCCGTCTAAGGGCGACGAGAACGAGATCGGCGCGAGCTTGGAGAACGCCGCGGCCTACAACGGGAATCTCGTCCGTCGGATTCTGAGCGGCGGCGGAACCCCGCTAAGGCCTAAAGCAGATCTGACCGTCTATTTATTTGCCCTTTTCAACGAGAACAAAAAGACCGGCCCCGCCTCCGAAAGAAACTACGGCCTATTTTACCCTAACGAGGAGAAAGTTTACGACATTCCGTTCACCACCGACGGGCTGAAGGACTACCACGACAAGCCATCGCCGGCGCCAGCAGCCGGCGGAGACGGCGGCCACACGGCGCCGGGGAGCGGCGACGGAGGCGTGTCGAAAAGTCAAACGGGGAACACGTGGTGCGTGGCGAGTGGTGAGGCCGGAAAAGAGAAACTGCAGGCGAGTCTAGATTACGCGTGTGGGGAAGGAGGGGCGGACTGCCGTCCGATTCAGCCGGATGCCACGTGTTACAATCCGAACACGCTGGAGGCCCACGCATCGTACGCCTTCAATTCTTATTATCAGAAGAACGGCCGCAAGATCGGCACGTGCTACTTCGGAGGGGCGGCGTACGTTGTCACGCAACCACCTAGTAAGTACTCTATAATTTCTCCTTGTTTTCGGGTAAGGCGTACGTTAGAGGATCCGACCCGAATAGACCCGCCCCGCATTTTGACGCTAAAATGA

mRNA sequence

ATGGACCCTCTAACTCCTCTCCCCTTCCTCATCTTCCTCCTCTGCCTCTCCCCATTTTCAGATGGTGGTTCCATTGGAATCAACTATGGCAGAATCGCCAACAACCTCCCTTCCGCCGTTAAGGTCGTTAAGCTCCTCAAATCCCACGGCCTCCACAGGGTCAAAGTTTACGACACCGATCCGGCCGTCCTACGGGCTCTCTCCGGCTCCGGCATTAAGGTCACCGTCGATTTGCCCAACGAGCTCCTCTTCGCCGCCGCCAAACGCCTCACCTTCGCCTACTCCTGGGTCGAGAAGAACGTCGCCGCCTACTACCCTTCCACTGAAATCGAAGCCATTGCCGTCGGAAACGAAGTCTTCGTCGACCCCCACAACACGACGTCGTTTCTTGTTCCGGCCATGAAGAACATTCACCAAGCCCTCGTCAAGTACAACCTCCACTCCGCCATTAAAATCTCTTCCCCCATCGCATTGAGCGCCTTGCAAAACTCCTACCCTTCCTCCGCCGGCTCCTTCCGGCCGGAGCTCGTCGAGACCGTTTTCCGGCCAATGCTGGACTTCCTCCGCCAAACCGGCTCCTACCTCATGGTAAACGCATACCCGTTCTTCGCCTACGAGTCCAACTCCGACGTCATTTCCTTAGACTACGCACTTTTCCGCGACAACCCCGGCGTGGTGGATTCCGGCAGCGGCTACCGGTACTTCAGCCTTTTCGACGCCCAAATCGACGCCGTTTTTGCCGCGATGTCGGCTCTCAAATACGACGACATTAAAATGGTGGTTACGGAGACCGGATGGCCGTCTAAGGGCGACGAGAACGAGATCGGCGCGAGCTTGGAGAACGCCGCGGCCTACAACGGGAATCTCGTCCGTCGGATTCTGAGCGGCGGCGGAACCCCGCTAAGGCCTAAAGCAGATCTGACCGTCTATTTATTTGCCCTTTTCAACGAGAACAAAAAGACCGGCCCCGCCTCCGAAAGAAACTACGGCCTATTTTACCCTAACGAGGAGAAAGTTTACGACATTCCGTTCACCACCGACGGGCTGAAGGACTACCACGACAAGCCATCGCCGGCGCCAGCAGCCGGCGGAGACGGCGGCCACACGGCGCCGGGGAGCGGCGACGGAGGCGTGTCGAAAAGTCAAACGGGGAACACGTGGTGCGTGGCGAGTGGTGAGGCCGGAAAAGAGAAACTGCAGGCGAGTCTAGATTACGCGTGTGGGGAAGGAGGGGCGGACTGCCGTCCGATTCAGCCGGATGCCACGTGTTACAATCCGAACACGCTGGAGGCCCACGCATCGTACGCCTTCAATTCTTATTATCAGAAGAACGGCCGCAAGATCGGCACGTGCTACTTCGGAGGGGCGGCGTACGTTGTCACGCAACCACCTAGTAAGTACTCTATAATTTCTCCTTGTTTTCGGGTAAGGCGTACGTTAGAGGATCCGACCCGAATAGACCCGCCCCGCATTTTGACGCTAAAATGA

Coding sequence (CDS)

ATGGACCCTCTAACTCCTCTCCCCTTCCTCATCTTCCTCCTCTGCCTCTCCCCATTTTCAGATGGTGGTTCCATTGGAATCAACTATGGCAGAATCGCCAACAACCTCCCTTCCGCCGTTAAGGTCGTTAAGCTCCTCAAATCCCACGGCCTCCACAGGGTCAAAGTTTACGACACCGATCCGGCCGTCCTACGGGCTCTCTCCGGCTCCGGCATTAAGGTCACCGTCGATTTGCCCAACGAGCTCCTCTTCGCCGCCGCCAAACGCCTCACCTTCGCCTACTCCTGGGTCGAGAAGAACGTCGCCGCCTACTACCCTTCCACTGAAATCGAAGCCATTGCCGTCGGAAACGAAGTCTTCGTCGACCCCCACAACACGACGTCGTTTCTTGTTCCGGCCATGAAGAACATTCACCAAGCCCTCGTCAAGTACAACCTCCACTCCGCCATTAAAATCTCTTCCCCCATCGCATTGAGCGCCTTGCAAAACTCCTACCCTTCCTCCGCCGGCTCCTTCCGGCCGGAGCTCGTCGAGACCGTTTTCCGGCCAATGCTGGACTTCCTCCGCCAAACCGGCTCCTACCTCATGGTAAACGCATACCCGTTCTTCGCCTACGAGTCCAACTCCGACGTCATTTCCTTAGACTACGCACTTTTCCGCGACAACCCCGGCGTGGTGGATTCCGGCAGCGGCTACCGGTACTTCAGCCTTTTCGACGCCCAAATCGACGCCGTTTTTGCCGCGATGTCGGCTCTCAAATACGACGACATTAAAATGGTGGTTACGGAGACCGGATGGCCGTCTAAGGGCGACGAGAACGAGATCGGCGCGAGCTTGGAGAACGCCGCGGCCTACAACGGGAATCTCGTCCGTCGGATTCTGAGCGGCGGCGGAACCCCGCTAAGGCCTAAAGCAGATCTGACCGTCTATTTATTTGCCCTTTTCAACGAGAACAAAAAGACCGGCCCCGCCTCCGAAAGAAACTACGGCCTATTTTACCCTAACGAGGAGAAAGTTTACGACATTCCGTTCACCACCGACGGGCTGAAGGACTACCACGACAAGCCATCGCCGGCGCCAGCAGCCGGCGGAGACGGCGGCCACACGGCGCCGGGGAGCGGCGACGGAGGCGTGTCGAAAAGTCAAACGGGGAACACGTGGTGCGTGGCGAGTGGTGAGGCCGGAAAAGAGAAACTGCAGGCGAGTCTAGATTACGCGTGTGGGGAAGGAGGGGCGGACTGCCGTCCGATTCAGCCGGATGCCACGTGTTACAATCCGAACACGCTGGAGGCCCACGCATCGTACGCCTTCAATTCTTATTATCAGAAGAACGGCCGCAAGATCGGCACGTGCTACTTCGGAGGGGCGGCGTACGTTGTCACGCAACCACCTAGTAAGTACTCTATAATTTCTCCTTGTTTTCGGGTAAGGCGTACGTTAGAGGATCCGACCCGAATAGACCCGCCCCGCATTTTGACGCTAAAATGA

Protein sequence

MDPLTPLPFLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPSKYSIISPCFRVRRTLEDPTRIDPPRILTLK

Homology

[Close]

BLAST of MS025058 vs. NCBI nr
Match: KAG6572415.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012014.1 Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 855.5 bits (2209), Expect = 2.2e-244
Identity = 427/464 (92.03%), Postives = 444/464 (95.69%), Query Frame = 0


Query: 1   MDPLTPLPFLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTD 60
           M+ LT L F IF+L LSPF++ GSIG+NYGRIANNLPSAVKVVKLLK+HGL RVKVYDTD
Sbjct: 1   MERLT-LSFFIFILFLSPFAESGSIGVNYGRIANNLPSAVKVVKLLKTHGLQRVKVYDTD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVF 120
           PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAY+WVEKNVAAYYPSTEIEAIAVGNEVF
Sbjct: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETV 180
           VDPHNTTSFLVPAMKNIHQALVK+NLHSAIK+SSPIALSALQNSYPSSAGSFRPELVE V
Sbjct: 121 VDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPV 180

Query: 181 FRPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
           FRPML+FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDA
Sbjct: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGAS+ NAAAYNGNL+RRILSGGGTP
Sbjct: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKK GP SERNYGLFYPNEE+VYDIPFTT+ LKDYHDKPSPAP
Sbjct: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAP 360

Query: 361 AAGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPD 420
           A GGD G T  GSGDGGVSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQP 
Sbjct: 361 ATGGD-GQTGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           ATCYNPNTL+AHASYAFNSYYQKN RKIGTCYFGGAAYVVTQPP
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPP 462

[Close]

BLAST of MS025058 vs. NCBI nr
Match: XP_022952844.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata])

HSP 1 Score: 850.9 bits (2197), Expect = 5.5e-243
Identity = 425/464 (91.59%), Postives = 443/464 (95.47%), Query Frame = 0


Query: 1   MDPLTPLPFLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTD 60
           M+ LT L F IFLL LSPF++ GSIG+NYGRIAN+LPSAVKVVKLLKSHGL RVKVYDTD
Sbjct: 1   MERLT-LSFFIFLLFLSPFAESGSIGVNYGRIANDLPSAVKVVKLLKSHGLQRVKVYDTD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVF 120
           PAVLRALSGSGIKVTVDLPNELLFAAAKRL+FAY+WVEKNVAAYYPSTEIEAIAVGNEVF
Sbjct: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETV 180
           VDPHNTTSFLVPAMKNIHQALVK+NLHSAIK+SSPIALSALQNSYPSSAGSFRPELVE V
Sbjct: 121 VDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPV 180

Query: 181 FRPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
           FRPML+FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDA
Sbjct: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDIK+VVTETGWPSKGDENEIGAS+ NAAAYNGNL+RRILSGGGTP
Sbjct: 241 QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKK GP SERNYGLFYPNEE+VYDIPFTT+ LKDYHDKPSPAP
Sbjct: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAP 360

Query: 361 AAGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPD 420
           A GGD G    GSGDGGVSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQP 
Sbjct: 361 ATGGD-GQAGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           ATCYNPNTL+AHASYAFNSYYQKN RKIGTCYFGGAAYVVTQPP
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPP 462

[Close]

BLAST of MS025058 vs. NCBI nr
Match: XP_023553980.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850.1 bits (2195), Expect = 9.4e-243
Identity = 426/464 (91.81%), Postives = 442/464 (95.26%), Query Frame = 0


Query: 1   MDPLTPLPFLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTD 60
           M+ LT L F IFLL L PF++ GSIG+NYGRIANNLPSAVKVVKLLKSHGL RVKVYDTD
Sbjct: 1   MERLT-LSFFIFLLFLYPFAESGSIGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVF 120
           PAVLRALSGSGIKVTVDLPNELLFAAAKRL+FA +WVEKNVAAYYPSTEIEAIAVGNEVF
Sbjct: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLSFANTWVEKNVAAYYPSTEIEAIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETV 180
           VDPHNTTSFLVPAMKNIHQALVK+NLHSAIK+SSPIALSALQNSYPSSAGSFRPELVE V
Sbjct: 121 VDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPV 180

Query: 181 FRPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
           FRPML+FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDA
Sbjct: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGAS+ NAAAYNGNL+RRILSGGGTP
Sbjct: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKK GP SERNYGLFYPNEE+VYDIPFTT+ LKDYHDKPSPAP
Sbjct: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAP 360

Query: 361 AAGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPD 420
           A GGD G T  GSGDGGVSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQP 
Sbjct: 361 ATGGD-GQTGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           ATCYNPNTL+AHASYAFNSYYQKN RKIGTCYFGGAAYVVTQPP
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPP 462

[Close]

BLAST of MS025058 vs. NCBI nr
Match: XP_022969398.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita maxima])

HSP 1 Score: 849.7 bits (2194), Expect = 1.2e-242
Identity = 426/464 (91.81%), Postives = 441/464 (95.04%), Query Frame = 0


Query: 1   MDPLTPLPFLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTD 60
           M+ LT L F IFLL L PF++ GSIG+NYGRIANNLPSAVKVVKLLKSHGL RVKVYDTD
Sbjct: 1   MERLT-LSFFIFLLFLYPFAESGSIGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVF 120
           PAVLRALSGSGIKVTVDLPNELLFAAAKRL+FAY+WVEKNVAAYYPSTEIEAIAVGNEVF
Sbjct: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETV 180
           VDPHNTTSFLVPAMKNIHQALVK+NLHSAIK+SSPIALSALQNSYPSSAGSFRPELVE V
Sbjct: 121 VDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPV 180

Query: 181 FRPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
           FRPML+FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDA
Sbjct: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGAS  NAAAYNGNL+RRILSGGGTP
Sbjct: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASAANAAAYNGNLIRRILSGGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKK GP SERNYGLFYPNEE+VYDIPFTT+ LKDYHDKPSPAP
Sbjct: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAP 360

Query: 361 AAGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPD 420
           A GGD G T  G GDGGVSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQP 
Sbjct: 361 ATGGD-GQTGSGHGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           ATCYNPNTL+AHASYAFNSYYQKN RKIGTCYFGGAAYVVTQPP
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNVRKIGTCYFGGAAYVVTQPP 462

[Close]

BLAST of MS025058 vs. NCBI nr
Match: XP_038886813.1 (LOW QUALITY PROTEIN: glucan endo-1,3-beta-glucosidase 12 [Benincasa hispida])

HSP 1 Score: 849.0 bits (2192), Expect = 2.1e-242
Identity = 423/461 (91.76%), Postives = 440/461 (95.44%), Query Frame = 0


Query: 9   FLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALS 68
           F IF L LS F++ GSIG+NYGRIANNLPSAVKVVKLLK+HGL RVKVYDTDPAVLRALS
Sbjct: 9   FFIFPLFLSSFAESGSIGVNYGRIANNLPSAVKVVKLLKTHGLQRVKVYDTDPAVLRALS 68

Query: 69  GSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 128
           GSGIKVTVDLPNELLFAAAKRLTFAY+WVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS
Sbjct: 69  GSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 128

Query: 129 FLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFL 188
           FLVPAMKNIHQALVKYNLHSAIK+SSPIALSALQNSYPSSAGSFRPELVETVFRPML+FL
Sbjct: 129 FLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLEFL 188

Query: 189 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAA 248
           RQTGSYLMVNAYPFFAYESN+DVISLDYALFRDNPGVVD+GSGYRYF+LFDAQIDAVFAA
Sbjct: 189 RQTGSYLMVNAYPFFAYESNADVISLDYALFRDNPGVVDAGSGYRYFNLFDAQIDAVFAA 248

Query: 249 MSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLT 308
           MSALKYDDIKMVVTETGWPSKGDENEIGAS+ENAAAYNGNLVRRIL+GGGTPLRPKADLT
Sbjct: 249 MSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILNGGGTPLRPKADLT 308

Query: 309 VYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGH 368
           VYLFALFNENKK GPASERNYGLFYPNEEKVY+IPFTT+GLKDYHDKPSPAP  GG+G  
Sbjct: 309 VYLFALFNENKKNGPASERNYGLFYPNEEKVYEIPFTTEGLKDYHDKPSPAPVTGGNGPS 368

Query: 369 TAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGG-----ADCRPIQPDATC 428
             P SGDGGVSKSQTGNTWCVASGEAGKEKLQ+SLDYACGEGG     ADCRPIQP ATC
Sbjct: 369 GPPESGDGGVSKSQTGNTWCVASGEAGKEKLQSSLDYACGEGGXXXXXADCRPIQPGATC 428

Query: 429 YNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           YNPNTLEAHASYAFNSYYQKN RK+GTCYFGGAAYVVTQPP
Sbjct: 429 YNPNTLEAHASYAFNSYYQKNSRKVGTCYFGGAAYVVTQPP 469

[Close]

BLAST of MS025058 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.4e-100
Identity = 197/470 (41.91%), Postives = 287/470 (61.06%), Query Frame = 0


Query: 7   LPFLIF--LLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVL 66
           L F IF  +L    F     IGI YGR A+NLPS  +V +L++   +  V++YD +  VL
Sbjct: 7   LVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVL 66

Query: 67  RALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPH 126
           +A + +GI++ + +PN  L A A+  +   +W+  N+  YYPST+I +I+VG EV   P 
Sbjct: 67  KAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPD 126

Query: 127 NTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETVFRPM 186
           N T  ++PAM+NIH AL K  L   IKISS  +L+ L  S+P S+ SF  +      +PM
Sbjct: 127 NATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAFLKPM 186

Query: 187 LDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDA 246
           L+FL +  S  M++ YP++AY  +++ + L+YALF  +  VVD  +G  Y ++FDAQ+DA
Sbjct: 187 LEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDA 246

Query: 247 VFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPK 306
           ++ A++A+ +  +K++VTE+GWPSKG   E  A+ ENA AYN NL+R ++   GTP +P 
Sbjct: 247 IYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPG 306

Query: 307 ADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFT---------TDGLKDYHDK 366
            ++ VYLF+LFNEN+K G  SERN+G+FY N   VY + FT         T+        
Sbjct: 307 EEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPS 366

Query: 367 PSPAPAAGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR 426
           PS +P   G+   T  G G GG  K      WC+AS +A   +LQ +LD+ACG G  DC 
Sbjct: 367 PSSSPIINGNSTVTIGGGGGGGTKK------WCIASSQASVTELQTALDWACGPGNVDCS 426

Query: 427 PIQPDATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPS 466
            +QPD  C+ P+T+ +HASYAFN+YYQ++G     C F GA+  V + PS
Sbjct: 427 AVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPS 469

[Close]

BLAST of MS025058 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 2.5e-97
Identity = 187/455 (41.10%), Postives = 279/455 (61.32%), Query Frame = 0


Query: 13  LLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALSGSGI 72
           LL L     GG +G+ YGR A++LP+  KVV+L++ H +  V++YD +  VL+A   + I
Sbjct: 14  LLLLLDCCYGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSI 73

Query: 73  KVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPH-NTTSFLV 132
           ++ + +PN  L A ++  +   +W++ +V  YYP+T+I  I VG E   DPH N +SF+V
Sbjct: 74  ELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVV 133

Query: 133 PAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFLRQT 192
           PAM+N+  AL K  L   IK+S+ ++L  L  S+P SAG+F         RPML+FL + 
Sbjct: 134 PAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYA-YFLRPMLEFLAEN 193

Query: 193 GSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAAMSA 252
            S  M++ YP++AY  + + +SLDY LF  +  V+D  +G  Y ++FDAQ+DA++ A++A
Sbjct: 194 KSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTA 253

Query: 253 LKYDDIKMVVTETGWPSKGDENE-IGASLENAAAYNGNLVRRILSGGGTPLRPKADLTVY 312
           L +  IK++VTETGWP+KG   E   AS +NA  YN N++R +++  GTP +P   + VY
Sbjct: 254 LNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVY 313

Query: 313 LFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGHTA 372
           +F+LFNEN+K G  SERN+GLFYP++  VY + FT                   +G H  
Sbjct: 314 IFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFT----------------GKSNGFH-- 373

Query: 373 PGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNTLE 432
             S   G + S + N+WC+AS +A +  L+ +LD+ACG G  DC  IQP   C+ P+TL 
Sbjct: 374 --SNSSGTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLV 433

Query: 433 AHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPS 466
           +HAS+ FNSY+Q+N      C FGGA   V + PS
Sbjct: 434 SHASFVFNSYFQQNRATDVACSFGGAGVKVNKDPS 447

[Close]

BLAST of MS025058 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 1.9e-92
Identity = 186/470 (39.57%), Postives = 276/470 (58.72%), Query Frame = 0


Query: 7   LPFLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRA 66
           L   +FL   S  S    IG+N G    N+PS  +VV LLKS  ++RV++YD D ++L A
Sbjct: 5   LLLFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLLA 64

Query: 67  LSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNT 126
            + +G++V + +PN+ L   ++    A +WV +NVAAYYP+T I  IAVG+EV     N 
Sbjct: 65  FAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTNA 124

Query: 127 TSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETVFRPMLD 186
            S LV A+K I  ALV  NL   IK+S+P + + + +S+P S   F  +  + V  P+L 
Sbjct: 125 ASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFN-KTWDPVIVPLLK 184

Query: 187 FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRD---NPGVVDSGSGYRYFSLFDAQID 246
           FL+ TGS L++N YP+F Y  ++ VI LDYALF+    N   VD+ +   Y ++FDA +D
Sbjct: 185 FLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIVD 244

Query: 247 AVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRP 306
           A + AMS L + +I +VVTE+GWPSKG  +E  A++ENA  YN NL++ +++  GTP  P
Sbjct: 245 AAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKHP 304

Query: 307 KADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAG 366
              +T Y++ L+NE+ + GP SE+N+GLFY N   VY +                     
Sbjct: 305 GTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRL------------------- 364

Query: 367 GDGGHTAPGSGDGGVSKSQTGN-TWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDAT 426
                    +G G +  + T N T+C+A  +  ++ LQA+LD+ACG G  DC  +    +
Sbjct: 365 ---------AGAGAILANDTTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGES 424

Query: 427 CYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPSKYSIISP 473
           CY P+ + AH++YAFN+YYQK G+  G+C F G A V T  PS+ + + P
Sbjct: 425 CYEPDDVVAHSTYAFNAYYQKMGKASGSCDFKGVATVTTTDPSRGTCVFP 445

[Close]

BLAST of MS025058 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 337.4 bits (864), Expect = 2.7e-91
Identity = 179/451 (39.69%), Postives = 263/451 (58.31%), Query Frame = 0


Query: 18  PFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALSGSGIKVTVD 77
           P  +G  IG+N G   +++P   +VV LLK+  +  +++Y+ DP +L AL+ +GIKV + 
Sbjct: 25  PADEGSYIGVNIGTDLSDMPHPTQVVALLKAQEIRHIRLYNADPGLLIALANTGIKVIIS 84

Query: 78  LPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSFLVPAMKNI 137
           +PN+ L    +  + A +WV++NV A+YP+T I A++VG+EV     N    LV A+KN+
Sbjct: 85  IPNDQLLGIGQSNSTAANWVKRNVIAHYPATMITAVSVGSEVLTSLSNAAPVLVSAIKNV 144

Query: 138 HQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFLRQTGSYLMV 197
           H AL+  NL   IK+S+P++ S + + +P S   F   L   V  P+L FL+ T SYLMV
Sbjct: 145 HAALLSANLDKLIKVSTPLSTSLILDPFPPSQAFFNRSL-NAVIVPLLSFLQSTNSYLMV 204

Query: 198 NAYPFFAYESNSDVISLDYALFR---DNPGVVDSGSGYRYFSLFDAQIDAVFAAMSALKY 257
           N YP+  Y  ++ VI LDYALF+    N   VD+ +  RY + FDA +DA + AM+ L +
Sbjct: 205 NVYPYIDYMQSNGVIPLDYALFKPIPPNKEAVDANTLVRYSNAFDAMVDATYFAMAFLNF 264

Query: 258 DDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFAL 317
            +I ++VTE+GWPSKG+ NE  A+L+NA  YN NL+R +L+  GTP RP   ++ Y++ L
Sbjct: 265 TNIPVLVTESGWPSKGETNEPDATLDNANTYNSNLIRHVLNKTGTPKRPGIAVSTYIYEL 324

Query: 318 FNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGHTAPGSG 377
           +NE+ K G  SE+N+GLF  N E VY +  T  G                          
Sbjct: 325 YNEDTKAG-LSEKNWGLFNANGEPVYVLRLTNSG-------------------------- 384

Query: 378 DGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNTLEAHAS 437
              ++   T  T+C A   A  + LQA+LD+ACG G  DC PI+   TCY P+ + AHA+
Sbjct: 385 -SVLANDTTNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVAHAN 444

Query: 438 YAFNSYYQKNGRKIGTCYFGGAAYVVTQPPS 466
           YAF++YY + G     C F G A + T  PS
Sbjct: 445 YAFDTYYHQTGNNPDACNFNGVASITTTDPS 446

[Close]

BLAST of MS025058 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.1e-89
Identity = 179/459 (39.00%), Postives = 266/459 (57.95%), Query Frame = 0


Query: 10  LIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALSG 69
           L+ LL +   S+   IG+N G    N+P    +V LLKS  +  V++YD +  +L+A + 
Sbjct: 11  LLLLLSILACSNAAFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFAN 70

Query: 70  SGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSF 129
           + I+V V + NE +    +  + A +WV KNVAAY PST I AIAVG+EV     +    
Sbjct: 71  TSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAPI 130

Query: 130 LVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFLR 189
           L  A+ NIH+ALV  NL+  +K+SSP+++  +   +P S  +F P    TV++ +L FL+
Sbjct: 131 LASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQ-LLQFLK 190

Query: 190 QTGSYLMVNAYPFFAYESNSDVISLDYALFRD-NP--GVVDSGSGYRYFSLFDAQIDAVF 249
            TGS+ M+NAYP++ Y + + +  LDYALF+  +P   +VD  +   Y S+FDA +DA +
Sbjct: 191 NTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAY 250

Query: 250 AAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKAD 309
            +M AL +  I +VVTETGWPS G  +E  A++ NA  +N NL++R+L+  G P +P   
Sbjct: 251 YSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIP 310

Query: 310 LTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDG 369
           +  Y++ L+NE+K++GP SERN+G+ +PN   VY                 P   +GG  
Sbjct: 311 INTYIYELYNEDKRSGPVSERNWGILFPNGTSVY-----------------PLSLSGGSS 370

Query: 370 GHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNP 429
                GS           + +CVA  +A  +KL   L++ACG+G A+C  IQP   CY P
Sbjct: 371 SAALNGS-----------SMFCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLP 430

Query: 430 NTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPS 466
           N +++HAS+AFN YYQK     GTC F G A   T+ PS
Sbjct: 431 NDVKSHASFAFNDYYQKMKSAGGTCDFDGTAITTTRDPS 440

[Close]

BLAST of MS025058 vs. ExPASy TrEMBL
Match: A0A6J1GMW6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111455410 PE=3 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 2.7e-243
Identity = 425/464 (91.59%), Postives = 443/464 (95.47%), Query Frame = 0


Query: 1   MDPLTPLPFLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTD 60
           M+ LT L F IFLL LSPF++ GSIG+NYGRIAN+LPSAVKVVKLLKSHGL RVKVYDTD
Sbjct: 1   MERLT-LSFFIFLLFLSPFAESGSIGVNYGRIANDLPSAVKVVKLLKSHGLQRVKVYDTD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVF 120
           PAVLRALSGSGIKVTVDLPNELLFAAAKRL+FAY+WVEKNVAAYYPSTEIEAIAVGNEVF
Sbjct: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETV 180
           VDPHNTTSFLVPAMKNIHQALVK+NLHSAIK+SSPIALSALQNSYPSSAGSFRPELVE V
Sbjct: 121 VDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPV 180

Query: 181 FRPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
           FRPML+FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDA
Sbjct: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDIK+VVTETGWPSKGDENEIGAS+ NAAAYNGNL+RRILSGGGTP
Sbjct: 241 QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKK GP SERNYGLFYPNEE+VYDIPFTT+ LKDYHDKPSPAP
Sbjct: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAP 360

Query: 361 AAGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPD 420
           A GGD G    GSGDGGVSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQP 
Sbjct: 361 ATGGD-GQAGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           ATCYNPNTL+AHASYAFNSYYQKN RKIGTCYFGGAAYVVTQPP
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPP 462

[Close]

BLAST of MS025058 vs. ExPASy TrEMBL
Match: A0A6J1I0V3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111468418 PE=3 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 6.0e-243
Identity = 426/464 (91.81%), Postives = 441/464 (95.04%), Query Frame = 0


Query: 1   MDPLTPLPFLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTD 60
           M+ LT L F IFLL L PF++ GSIG+NYGRIANNLPSAVKVVKLLKSHGL RVKVYDTD
Sbjct: 1   MERLT-LSFFIFLLFLYPFAESGSIGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVF 120
           PAVLRALSGSGIKVTVDLPNELLFAAAKRL+FAY+WVEKNVAAYYPSTEIEAIAVGNEVF
Sbjct: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETV 180
           VDPHNTTSFLVPAMKNIHQALVK+NLHSAIK+SSPIALSALQNSYPSSAGSFRPELVE V
Sbjct: 121 VDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPV 180

Query: 181 FRPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
           FRPML+FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDA
Sbjct: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGAS  NAAAYNGNL+RRILSGGGTP
Sbjct: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASAANAAAYNGNLIRRILSGGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKK GP SERNYGLFYPNEE+VYDIPFTT+ LKDYHDKPSPAP
Sbjct: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAP 360

Query: 361 AAGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPD 420
           A GGD G T  G GDGGVSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQP 
Sbjct: 361 ATGGD-GQTGSGHGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           ATCYNPNTL+AHASYAFNSYYQKN RKIGTCYFGGAAYVVTQPP
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNVRKIGTCYFGGAAYVVTQPP 462

[Close]

BLAST of MS025058 vs. ExPASy TrEMBL
Match: A0A0A0KEL0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G421570 PE=3 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.2e-240
Identity = 416/456 (91.23%), Postives = 434/456 (95.18%), Query Frame = 0


Query: 9   FLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLRALS 68
           F  FLL LSPF++ GSIG+NYGRI N+LPSAVKVVKLLKSHGL RVKVYDTDPAVL+ALS
Sbjct: 10  FYFFLLFLSPFAESGSIGVNYGRIGNDLPSAVKVVKLLKSHGLQRVKVYDTDPAVLKALS 69

Query: 69  GSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 128
           GSGIKVTVDLPNELLFAAAKRLTFAY+WVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS
Sbjct: 70  GSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTS 129

Query: 129 FLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETVFRPMLDFL 188
           FLVPAMKNIHQALVKYNLHS IK+SSPIALSALQNSYPSSAGSFRPELVETVFRPML+FL
Sbjct: 130 FLVPAMKNIHQALVKYNLHSNIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLEFL 189

Query: 189 RQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAA 248
           RQTGSYLMVNAYPFFAYESN+DVISLDYALFRDNPGVVD+GSGYRYF+LFDAQIDAVFAA
Sbjct: 190 RQTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDAGSGYRYFNLFDAQIDAVFAA 249

Query: 249 MSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKADLT 308
           MSALKYDDIKMVVTETGWPSKGDENEIGAS+ENAAAYNGNLVRRILSGGGTPLRPKADLT
Sbjct: 250 MSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLT 309

Query: 309 VYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGDGGH 368
           VYLFALFNENKK GP SERNYGLFYPNEEKVYDIPFTT+GLKD+ DKPSP P +GG+   
Sbjct: 310 VYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTTEGLKDFEDKPSPKPVSGGNAPT 369

Query: 369 TAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYNPNT 428
             P SGDGGVSKSQTGNTWCVASGEAGKEKLQ+ LDYACGEGGADCRPIQ  ATCYNPNT
Sbjct: 370 APPASGDGGVSKSQTGNTWCVASGEAGKEKLQSGLDYACGEGGADCRPIQVGATCYNPNT 429

Query: 429 LEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           LEAHASYAFNSYYQKN RK+GTCYFGGAAYVVTQPP
Sbjct: 430 LEAHASYAFNSYYQKNSRKVGTCYFGGAAYVVTQPP 465

[Close]

BLAST of MS025058 vs. ExPASy TrEMBL
Match: A0A6J1ERV0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437224 PE=3 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 4.9e-237
Identity = 416/470 (88.51%), Postives = 439/470 (93.40%), Query Frame = 0


Query: 1   MDPLTPLPFLIFLLCLSP----FSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKV 60
           M+ LT   F +FLL L+      ++ GSIG+NYGRIANNLPSA+KVV LLKSHGL RVKV
Sbjct: 1   MERLTLSFFFVFLLLLAASFPVLTEAGSIGVNYGRIANNLPSALKVVNLLKSHGLQRVKV 60

Query: 61  YDTDPAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVG 120
           YDTDPAVLRA+SGSGIKVTVDLPNELLFAAAKRLTFAY+WVEKN+ AYYPSTEIEAIAVG
Sbjct: 61  YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNIVAYYPSTEIEAIAVG 120

Query: 121 NEVFVDPHNTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPEL 180
           NEVFVDPHNTTSFL+PAMKNIHQALVKYNLHS+IK+SSPIALSALQNSYPSSAGSFRPEL
Sbjct: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180

Query: 181 VETVFRPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFS 240
           +E+VFRPML+FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVD+GSGYRYFS
Sbjct: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240

Query: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSG 300
           LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGAS+ENAAAYNGNLVRRILSG
Sbjct: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG 300

Query: 301 GGTPLRPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDK- 360
           GGTPLRPKADLTVYLFALFNENKK GP SERNYGLFYPNEE VYDIPFTT+GLKDYHDK 
Sbjct: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360

Query: 361 -PSPAPAAGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADC 420
            PSP+PA GGD G + P +GDG VSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADC
Sbjct: 361 SPSPSPATGGD-GKSGPVNGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADC 420

Query: 421 RPIQPDATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           RPIQ  ATCYNPNTLEAHASYAFNSYYQKNGR  GTCYFGGAAYVVTQPP
Sbjct: 421 RPIQVGATCYNPNTLEAHASYAFNSYYQKNGRMTGTCYFGGAAYVVTQPP 469

[Close]

BLAST of MS025058 vs. ExPASy TrEMBL
Match: A0A6J1JLE7 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111486266 PE=3 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.4e-236
Identity = 416/470 (88.51%), Postives = 438/470 (93.19%), Query Frame = 0


Query: 1   MDPLTPLPFLIFLLCLSP----FSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKV 60
           M+ LT   F +FLL LSP     ++ GSIG+NYGRIANNLPSAVKVV LLKSHGL RVKV
Sbjct: 1   MERLTLSFFFVFLLLLSPSFAVVTEAGSIGVNYGRIANNLPSAVKVVNLLKSHGLQRVKV 60

Query: 61  YDTDPAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVG 120
           YDTDPAVL A+SGSGIKVTVDLPNELLFAAAKRLTFAY+WVEKN+ AYYPSTEIEAIAVG
Sbjct: 61  YDTDPAVLTAISGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNIVAYYPSTEIEAIAVG 120

Query: 121 NEVFVDPHNTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPEL 180
           NEVFVDPHNTTSFL+PAMKNIHQALVKYNLHS+IK+SSPIALSALQNSYPSSAGSFRPEL
Sbjct: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180

Query: 181 VETVFRPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFS 240
           +E+VFRPML+FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVD+GSGYRYFS
Sbjct: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240

Query: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSG 300
           LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGAS+ NAAAYNGNLVRRILSG
Sbjct: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVGNAAAYNGNLVRRILSG 300

Query: 301 GGTPLRPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDK- 360
           GGTPLRPKADLTVYLFALFNENKK GP SERNYGLFYPNEE VYDIPFTT+GLKDYHDK 
Sbjct: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360

Query: 361 -PSPAPAAGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADC 420
            PSP+PA GGD G + P +GDG VSKSQ+GNTWCVASGEAGKEKLQASLDYACGEGGADC
Sbjct: 361 SPSPSPATGGD-GKSGPVNGDGNVSKSQSGNTWCVASGEAGKEKLQASLDYACGEGGADC 420

Query: 421 RPIQPDATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           RPIQ  ATCYNPNTLEAHASYAFNSYYQKNGRK GTCYFGGAAYVVTQ P
Sbjct: 421 RPIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQQP 469

[Close]

BLAST of MS025058 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 713.4 bits (1840), Expect = 1.3e-205
Identity = 352/464 (75.86%), Postives = 407/464 (87.72%), Query Frame = 0


Query: 1   MDPLTPLPFLIFLLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTD 60
           MD LTPL FL+ LL     SD GSIG+NYGRI++ LPSA KVV+LLKS G+ RVK++D D
Sbjct: 1   MDLLTPLIFLLLLLSPFSLSDAGSIGVNYGRISDELPSAFKVVQLLKSQGITRVKIFDAD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVF 120
           P+VL+ALSGSGIKVTVDLPNELLF+AAKR +FA SWV++NVAAY+PST+IE+IAVGNEVF
Sbjct: 61  PSVLKALSGSGIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETV 180
           VD HNTTSFL+PAM+NIH+AL+ +NLHS IKISSP+ALSALQNSYPSS+GSFRPEL+++V
Sbjct: 121 VDTHNTTSFLIPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSV 180

Query: 181 FRPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
            +PMLDFLR+TGS LM+N YPFFAYE NSDVI LDYAL R+NPG+VDSG+G RYF+LFDA
Sbjct: 181 IKPMLDFLRETGSRLMINVYPFFAYEGNSDVIPLDYALLRENPGMVDSGNGLRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDI+++VTETGWPSKGDENE+GA+L NAA+YNGNL+RRIL+ GGTP
Sbjct: 241 QIDAVFAAMSALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGNLIRRILTRGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKK GP SERNYGLF+P+E+KVYDIPFTT+GLK Y D     P
Sbjct: 301 LRPKADLTVYLFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRD-GGHTP 360

Query: 361 AAGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPD 420
             GGD     P S  GGVSKS  G TWCVA+G+AG+E+LQ  LDYACGEGGADCRPIQP 
Sbjct: 361 VTGGDQVTKPPMS--GGVSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPG 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           A CY+P+TLEAHAS+AFNSYYQK GR  G+CYFGGAAYVV+QPP
Sbjct: 421 ANCYSPDTLEAHASFAFNSYYQKKGRAGGSCYFGGAAYVVSQPP 461

[Close]

BLAST of MS025058 vs. TAIR 10
Match: AT5G55180.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 598.6 bits (1542), Expect = 4.6e-171
Identity = 301/465 (64.73%), Postives = 357/465 (76.77%), Query Frame = 0


Query: 9   FLIFLLCLSPFS-------DGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDP 68
           F++ LL LS FS       D G IG+NYGRIA+NLP+  KVV+LLK+ G++R+K+YDT+ 
Sbjct: 4   FVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYDTET 63

Query: 69  AVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFV 128
            VL AL+ SGIKV V LPNE L +AA   ++  +WV+ N+  Y P+T+IEAIAVGNEVFV
Sbjct: 64  TVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNEVFV 123

Query: 129 DPHNTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETVF 188
           DP NTT++LVPAMKN+  +LVK+NL  +IKISSPIALSAL +SYP SAGSF+PEL+E V 
Sbjct: 124 DPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIEPVI 183

Query: 189 RPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQ 248
           +PMLD LR+T S+LMVNAYPFFAY +N+D ISLDYALF++N G VDSG+G +Y SL DAQ
Sbjct: 184 KPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLLDAQ 243

Query: 249 IDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPL 308
           IDAVFAAMSA+ ++D+K+VVTETGWPS GDENEIGA   NAAAYNG LV+R+L+G GTPL
Sbjct: 244 IDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNGTPL 303

Query: 309 RPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPA 368
           +PK  L VYLFALFNEN+KTGP SERNYGLFYPNE KVYD+           +K    P 
Sbjct: 304 KPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSLNGKSTPVNDNKEKVVP- 363

Query: 369 AGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDA 428
                           V  S  G TWCVA+G+  KEKLQ  LDYACGEGGADCRPIQP A
Sbjct: 364 ----------------VKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGA 423

Query: 429 TCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPSK 467
           TCYNP +LEAHASYAFNSYYQKN R +GTC FGGAAYVV+QPPSK
Sbjct: 424 TCYNPESLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPPSK 451

[Close]

BLAST of MS025058 vs. TAIR 10
Match: AT5G55180.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 594.7 bits (1532), Expect = 6.6e-170
Identity = 299/463 (64.58%), Postives = 355/463 (76.67%), Query Frame = 0


Query: 9   FLIFLLCLSPFS-------DGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDP 68
           F++ LL LS FS       D G IG+NYGRIA+NLP+  KVV+LLK+ G++R+K+YDT+ 
Sbjct: 4   FVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYDTET 63

Query: 69  AVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFV 128
            VL AL+ SGIKV V LPNE L +AA   ++  +WV+ N+  Y P+T+IEAIAVGNEVFV
Sbjct: 64  TVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNEVFV 123

Query: 129 DPHNTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETVF 188
           DP NTT++LVPAMKN+  +LVK+NL  +IKISSPIALSAL +SYP SAGSF+PEL+E V 
Sbjct: 124 DPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIEPVI 183

Query: 189 RPMLDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQ 248
           +PMLD LR+T S+LMVNAYPFFAY +N+D ISLDYALF++N G VDSG+G +Y SL DAQ
Sbjct: 184 KPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLLDAQ 243

Query: 249 IDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPL 308
           IDAVFAAMSA+ ++D+K+VVTETGWPS GDENEIGA   NAAAYNG LV+R+L+G GTPL
Sbjct: 244 IDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNGTPL 303

Query: 309 RPKADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPA 368
           +PK  L VYLFALFNEN+KTGP SERNYGLFYPNE KVYD+           +K    P 
Sbjct: 304 KPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSLNGKSTPVNDNKEKVVP- 363

Query: 369 AGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDA 428
                           V  S  G TWCVA+G+  KEKLQ  LDYACGEGGADCRPIQP A
Sbjct: 364 ----------------VKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGA 423

Query: 429 TCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           TCYNP +LEAHASYAFNSYYQKN R +GTC FGGAAYVV+QPP
Sbjct: 424 TCYNPESLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPP 449

[Close]

BLAST of MS025058 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 582.0 bits (1499), Expect = 4.5e-166
Identity = 297/459 (64.71%), Postives = 355/459 (77.34%), Query Frame = 0


Query: 7   LPFLIFLLCLSPF-SDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVLR 66
           LP+ + L  LS   +  G +G+NYGRIANNLPS  KVV LLKS G++R+K++DTD  VL 
Sbjct: 6   LPYFLILSFLSAIDAHSGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDTDKNVLT 65

Query: 67  ALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPHN 126
           AL+ S IKV V LPNELL +AA   +FA +W++ ++  Y+P+TEIEAIAVGNEVFVDP  
Sbjct: 66  ALANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEVFVDP-T 125

Query: 127 TTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETVFRPML 186
            T +LV AMKNIH +LVKY L  AIKISSPIALSAL NSYP S+GSF+PEL+E V +PML
Sbjct: 126 ITPYLVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIEPVVKPML 185

Query: 187 DFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAV 246
             L+QT SYLMVNAYPFFAY +N+D ISLDYALF++N G +DSG+G +Y SLFDAQIDAV
Sbjct: 186 ALLQQTSSYLMVNAYPFFAYAANADKISLDYALFKENAGNIDSGTGLKYNSLFDAQIDAV 245

Query: 247 FAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPKA 306
           +AA+SA+ +  +K++VTETGWPS GDENEIGAS  NAAAYN  LV+R+L+G GTPLRP  
Sbjct: 246 YAALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGKGTPLRPTE 305

Query: 307 DLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFTTDGLKDYHDKPSPAPAAGGD 366
            L VYLFALFNEN+K GP SERNYGLFYPNE KVY++PFT         K S  P  G  
Sbjct: 306 PLNVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFT---------KKSTTPVNG-- 365

Query: 367 GGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQPDATCYN 426
                     G V  +  G+TWCV++GE  KEKLQ +LDYACGEGGADCRPIQP ATCY+
Sbjct: 366 --------NRGKVPVTHEGHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYH 425

Query: 427 PNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPP 465
           P +LEAHASYAFNSYYQKN R++GTC+FGGAA+VVTQPP
Sbjct: 426 PESLEAHASYAFNSYYQKNSRRVGTCFFGGAAHVVTQPP 444

[Close]

BLAST of MS025058 vs. TAIR 10
Match: AT4G29360.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 372.9 bits (956), Expect = 4.1e-103
Identity = 199/472 (42.16%), Postives = 289/472 (61.23%), Query Frame = 0


Query: 7   LPFLIF--LLCLSPFSDGGSIGINYGRIANNLPSAVKVVKLLKSHGLHRVKVYDTDPAVL 66
           L F IF  +L    F     IGI YGR A+NLPS  +V +L++   +  V++YD +  VL
Sbjct: 7   LVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVL 66

Query: 67  RALSGSGIKVTVDLPNELLFAAAKRLTFAYSWVEKNVAAYYPSTEIEAIAVGNEVFVDPH 126
           +A + +GI++ + +PN  L A A+  +   +W+  N+  YYPST+I +I+VG EV   P 
Sbjct: 67  KAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPD 126

Query: 127 NTTSFLVPAMKNIHQALVKYNLHSAIKISSPIALSALQNSYPSSAGSFRPELVETVFRPM 186
           N T  ++PAM+NIH AL K  L   IKISS  +L+ L  S+P S+ SF  +      +PM
Sbjct: 127 NATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAFLKPM 186

Query: 187 LDFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDA 246
           L+FL +  S  M++ YP++AY  +++ + L+YALF  +  VVD  +G  Y ++FDAQ+DA
Sbjct: 187 LEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDA 246

Query: 247 VFAAMSALKYDDIKMVVTETGWPSKGDENEIGASLENAAAYNGNLVRRILSGGGTPLRPK 306
           ++ A++A+ +  +K++VTE+GWPSKG   E  A+ ENA AYN NL+R ++   GTP +P 
Sbjct: 247 IYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPG 306

Query: 307 ADLTVYLFALFNENKKTGPASERNYGLFYPNEEKVYDIPFT---------TDGLKDYHDK 366
            ++ VYLF+LFNEN+K G  SERN+G+FY N   VY + FT         T+        
Sbjct: 307 EEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPS 366

Query: 367 PSPAPAAGGDGGHTAPGSGDGGVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR 426
           PS +P   G+   T  G G GG  K      WC+AS +A   +LQ +LD+ACG G  DC 
Sbjct: 367 PSSSPIINGNSTVTIGGGGGGGTKK------WCIASSQASVTELQTALDWACGPGNVDCS 426

Query: 427 PIQPDATCYNPNTLEAHASYAFNSYYQKNGRKIGTCYFGGAAYVVTQPPSKY 468
            +QPD  C+ P+T+ +HASYAFN+YYQ++G     C F GA+  V + PSKY
Sbjct: 427 AVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSKY 471

The following BLAST results are available for this feature:

BLAST of MS025058 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6572415.1	2.2e-244	92.03	Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022952844.1	5.5e-243	91.59	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata]	[more]
XP_023553980.1	9.4e-243	91.81	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita pepo subsp. pepo]	[more]
XP_022969398.1	1.2e-242	91.81	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita maxima]	[more]
XP_038886813.1	2.1e-242	91.76	LOW QUALITY PROTEIN: glucan endo-1,3-beta-glucosidase 12 [Benincasa hispida]	[more]

BLAST of MS025058 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYE5	1.4e-100	41.91	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9FJU9	2.5e-97	41.10	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q9ZU91	1.9e-92	39.57	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
Q9C7U5	2.7e-91	39.69	Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ...	[more]
Q94CD8	1.1e-89	39.00	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]

BLAST of MS025058 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GMW6	2.7e-243	91.59	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111455410 P...	[more]
A0A6J1I0V3	6.0e-243	91.81	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111468418 PE=...	[more]
A0A0A0KEL0	1.2e-240	91.23	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G421570 PE=3...	[more]
A0A6J1ERV0	4.9e-237	88.51	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437224 P...	[more]
A0A6J1JLE7	2.4e-236	88.51	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111486266 PE=...	[more]

BLAST of MS025058 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G05790.1	1.3e-205	75.86	O-Glycosyl hydrolases family 17 protein	[more]
AT5G55180.2	4.6e-171	64.73	O-Glycosyl hydrolases family 17 protein	[more]
AT5G55180.1	6.6e-170	64.58	O-Glycosyl hydrolases family 17 protein	[more]
AT4G26830.1	4.5e-166	64.71	O-Glycosyl hydrolases family 17 protein	[more]
AT4G29360.2	4.1e-103	42.16	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 386..470 e-value: 1.9E-44 score: 163.7
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 386..457 e-value: 1.2E-22 score: 80.4
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 25..344 e-value: 1.0E-89 score: 301.3
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 257..270
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 23..353 e-value: 1.2E-125 score: 420.8
None	No IPR available	GENE3D	1.20.58.1040		coord: 381..485 e-value: 4.8E-12 score: 48.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 350..380
None	No IPR available	PANTHER	PTHR32227:SF350	O-GLYCOSYL HYDROLASES FAMILY 17 PROTEIN-RELATED	coord: 11..469
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 11..469
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 25..343

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS025058.1	MS025058.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds