MS025005 (gene) Bitter gourd (TR) v1

BLAST of MS025005 vs. NCBI nr
Match: XP_022139896.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X2 [Momordica charantia])

HSP 1 Score: 1568.9 bits (4061), Expect = 0.0e+00
Identity = 770/816 (94.36%), Postives = 772/816 (94.61%), Query Frame = 0

Query: 123 MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF 182
           MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF
Sbjct: 1   MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF 60

Query: 183 TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS 242
           TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS
Sbjct: 61  TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS 120

Query: 243 SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL 302
           SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL
Sbjct: 121 SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL 180

Query: 303 TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP 362
           TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP
Sbjct: 181 TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP 240

Query: 363 KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC 422
           KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC
Sbjct: 241 KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC 300

Query: 423 GDFGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLP 482
           GDFGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLP
Sbjct: 301 GDFGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLP 360

Query: 483 DSSGYLVNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQD 542
           DSSGYLVNVN SIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQD
Sbjct: 361 DSSGYLVNVNKSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQD 420

Query: 543 LYVRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRG------------ 602
           LYVRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRG            
Sbjct: 421 LYVRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRGNEITASEIQSQE 480

Query: 603 -----------------KHFSSNTLIN-------LSGKLPCGQEIAVKRLAEGSGQGQSE 662
                             +FS +  I          GKLPCGQEIAVKRLAEGSGQGQSE
Sbjct: 481 NEVEMPLYDFTTIETATNYFSFSNKIGEGGFGPVYKGKLPCGQEIAVKRLAEGSGQGQSE 540

Query: 663 FRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLNWKKRV 722
           FRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLNWKKRV
Sbjct: 541 FRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLNWKKRV 600

Query: 723 DIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQTMTKTQ 782
           DIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQTMTKTQ
Sbjct: 601 DIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQTMTKTQ 660

Query: 783 RIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLGHAWKL 842
           RIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLGHAWKL
Sbjct: 661 RIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLGHAWKL 720

Query: 843 WDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESENMVLSH 902
           WDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESENMVLSH
Sbjct: 721 WDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESENMVLSH 780

BLAST of MS025005 vs. NCBI nr
Match: XP_022139895.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia])

HSP 1 Score: 1455.3 bits (3766), Expect = 0.0e+00
Identity = 705/820 (85.98%), Postives = 746/820 (90.98%), Query Frame = 0

Query: 123 MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF 182
           MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF
Sbjct: 1   MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF 60

Query: 183 TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS 242
           TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS
Sbjct: 61  TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS 120

Query: 243 SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL 302
           SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL
Sbjct: 121 SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL 180

Query: 303 TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP 362
           TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP
Sbjct: 181 TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP 240

Query: 363 KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC 422
           KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC
Sbjct: 241 KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC 300

Query: 423 GDFGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLP 482
           GDFGICTFSLTAQCDCMAGFEPKSP+ WE+  WSDGCVRRD +IC NGEGFKRI SVKLP
Sbjct: 301 GDFGICTFSLTAQCDCMAGFEPKSPESWERFMWSDGCVRRDIRICRNGEGFKRIRSVKLP 360

Query: 483 DSSGYLVNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQD 542
           DSSG+LVNVNTSIQDC+AACLNNCSCLAYGIME+ TGGYGCV WFQKL+DVRFV +NGQD
Sbjct: 361 DSSGHLVNVNTSIQDCKAACLNNCSCLAYGIMELSTGGYGCVTWFQKLVDVRFVLQNGQD 420

Query: 543 LYVRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRGKHFSSN------ 602
           LYVRVAASELDST KKL+VAISVSV SFLGF VLVIC +LRRR+K RG   +        
Sbjct: 421 LYVRVAASELDSTKKKLIVAISVSVVSFLGFSVLVICIVLRRRKKARGNEIAPEITAGEF 480

Query: 603 --------------TLINLS--------------------GKLPCGQEIAVKRLAEGSGQ 662
                         T+I  +                    GKLPCGQEIAVKRLAEGSGQ
Sbjct: 481 QSQENEVEMPIYDFTMIETATNYFSFANKIGEGGFGPVYKGKLPCGQEIAVKRLAEGSGQ 540

Query: 663 GQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLNW 722
           GQSEF+NEVLLISQLQHRNLVKLLGFCIH+EETLLVYEYMPNKSLD+FLFDN+RRSLL+W
Sbjct: 541 GQSEFKNEVLLISQLQHRNLVKLLGFCIHQEETLLVYEYMPNKSLDYFLFDNKRRSLLDW 600

Query: 723 KKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQTM 782
           KKR+DIIIG+ARGLLYLHRDSRLRIIHRDLKVSNILLD+EMNPKISDFGMARMF ED+TM
Sbjct: 601 KKRLDIIIGIARGLLYLHRDSRLRIIHRDLKVSNILLDDEMNPKISDFGMARMFAEDETM 660

Query: 783 TKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLGH 842
           TKT+R+VGT+GYMSPEY LDG FSLKSD+FSFGVILLEI+SG KNRGFFH +HQLNLLGH
Sbjct: 661 TKTKRVVGTYGYMSPEYALDGYFSLKSDVFSFGVILLEIVSGKKNRGFFHSEHQLNLLGH 720

Query: 843 AWKLWDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESENM 902
           AWKLWD+ NALELMDAAL+D+FQPSEA+RCIQVGLLCVQQ+PDERP+MWSV+SMLESENM
Sbjct: 721 AWKLWDEENALELMDAALKDQFQPSEAVRCIQVGLLCVQQNPDERPTMWSVISMLESENM 780

BLAST of MS025005 vs. NCBI nr
Match: XP_022139897.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X3 [Momordica charantia])

HSP 1 Score: 1291.2 bits (3340), Expect = 0.0e+00
Identity = 633/821 (77.10%), Postives = 703/821 (85.63%), Query Frame = 0

Query: 123 MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF 182
           M KLICRC LS   FF TTIALF RKSFASD I  KAGES+N ST+ILVS+ QNFVLG+F
Sbjct: 1   MSKLICRCKLSAFLFFLTTIALFPRKSFASDII--KAGESLNVSTQILVSSRQNFVLGMF 60

Query: 183 TPQGSKFEYLGIWYNYKN-IPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLW 242
           +PQGSKF+YLGIW  YKN IPQTVVWVANRDNP+VNSSARL F ++GNIVLLNETGGVLW
Sbjct: 61  SPQGSKFQYLGIW--YKNIIPQTVVWVANRDNPIVNSSARLTF-KDGNIVLLNETGGVLW 120

Query: 243 SSASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRK 302
           SS S  +VKEPV QLLDTGNL+LR+ GSENYVWQSFDYP DTLLPGMKLGWDSKTGLNRK
Sbjct: 121 SSTS--SVKEPVAQLLDTGNLVLRDSGSENYVWQSFDYPFDTLLPGMKLGWDSKTGLNRK 180

Query: 303 LTSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYF 362
           LTSW+S NDPSSGDF YS+ ++GLPQ +IRKG  TT+RGGPWYGTRFSG+ PLR+   Y 
Sbjct: 181 LTSWKSSNDPSSGDFTYSLDVNGLPQFLIRKGSITTFRGGPWYGTRFSGTAPLRDTAIYS 240

Query: 363 PKFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGL 422
           PKF+Y A E  +SYDA NNL +RL LN+ G+F+QFYWDDGGK+WY +Y +PGDRCDDYGL
Sbjct: 241 PKFDYRADEAFFSYDAVNNLIVRLALNSAGSFQQFYWDDGGKFWYSMYAMPGDRCDDYGL 300

Query: 423 CGDFGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKL 482
           CGDFGICTFSLTAQCDCMAGFEPKSP+ WE+  WSDGCVRRD +IC NGEGFKRI SVKL
Sbjct: 301 CGDFGICTFSLTAQCDCMAGFEPKSPESWERFMWSDGCVRRDIRICRNGEGFKRIRSVKL 360

Query: 483 PDSSGYLVNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQ 542
           PDSSG+LVNVNTSIQDC+AACLNNCSCLAYGIME+ TGGYGCV WFQKL+DVRFV +NGQ
Sbjct: 361 PDSSGHLVNVNTSIQDCKAACLNNCSCLAYGIMELSTGGYGCVTWFQKLVDVRFVLQNGQ 420

Query: 543 DLYVRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRGKHFSSN----- 602
           DLYVRVAASELDST KKL+VAISVSV SFLGF VLVIC +LRRR+K RG   +       
Sbjct: 421 DLYVRVAASELDSTKKKLIVAISVSVVSFLGFSVLVICIVLRRRKKARGNEIAPEITAGE 480

Query: 603 ---------------TLINLS--------------------GKLPCGQEIAVKRLAEGSG 662
                          T+I  +                    GKLPCGQEIAVKRLAEGSG
Sbjct: 481 FQSQENEVEMPIYDFTMIETATNYFSFANKIGEGGFGPVYKGKLPCGQEIAVKRLAEGSG 540

Query: 663 QGQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLN 722
           QGQSEF+NEVLLISQLQHRNLVKLLGFCIH+EETLLVYEYMPNKSLD+FLFDN+RRSLL+
Sbjct: 541 QGQSEFKNEVLLISQLQHRNLVKLLGFCIHQEETLLVYEYMPNKSLDYFLFDNKRRSLLD 600

Query: 723 WKKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQT 782
           WKKR+DIIIG+ARGLLYLHRDSRLRIIHRDLKVSNILLD+EMNPKISDFGMARMF ED+T
Sbjct: 601 WKKRLDIIIGIARGLLYLHRDSRLRIIHRDLKVSNILLDDEMNPKISDFGMARMFAEDET 660

Query: 783 MTKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLG 842
           MTKT+R+VGT+GYMSPEY LDG FSLKSD+FSFGVILLEI+SG KNRGFFH +HQLNLLG
Sbjct: 661 MTKTKRVVGTYGYMSPEYALDGYFSLKSDVFSFGVILLEIVSGKKNRGFFHSEHQLNLLG 720

Query: 843 HAWKLWDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESEN 902
           HAWKLWD+ NALELMDAAL+D+FQPSEA+RCIQVGLLCVQQ+PDERP+MWSV+SMLESEN
Sbjct: 721 HAWKLWDEENALELMDAALKDQFQPSEAVRCIQVGLLCVQQNPDERPTMWSVISMLESEN 780

BLAST of MS025005 vs. NCBI nr
Match: KGN64613.2 (hypothetical protein Csa_014353 [Cucumis sativus])

HSP 1 Score: 1288.9 bits (3334), Expect = 0.0e+00
Identity = 630/816 (77.21%), Postives = 692/816 (84.80%), Query Frame = 0

Query: 117 LILKEAMGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQN 176
           L LKEAM +LI +C L    F  TTIALF  KS   DSI  KAGESINGST+ILVSA+QN
Sbjct: 86  LNLKEAMRELISKCKLLAFLFLLTTIALFPTKSHGKDSI--KAGESINGSTQILVSAKQN 145

Query: 177 FVLGIFTPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNET 236
           FVLGIFTPQGSKF+YLGIW+N  NIPQT+VWVANRDNPLVNSS +L+F   GNIVLLNET
Sbjct: 146 FVLGIFTPQGSKFQYLGIWFN--NIPQTIVWVANRDNPLVNSSGKLEF-RRGNIVLLNET 205

Query: 237 GGVLWSSASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKT 296
            G+LWSS S  T K+PV QLLDTGN ++RE GSE+YVWQSF+YPSDTLLPGMKLGW SKT
Sbjct: 206 DGILWSSISPGTPKDPVAQLLDTGNWVVRESGSEDYVWQSFNYPSDTLLPGMKLGWSSKT 265

Query: 297 GLNRKLTSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRE 356
           GLNRKL SW+SLNDPS GDF YSV L+GLPQLV R+G   TYRGGPWYG RFSGS PLR+
Sbjct: 266 GLNRKLRSWKSLNDPSFGDFTYSVDLNGLPQLVTREGLIITYRGGPWYGNRFSGSAPLRD 325

Query: 357 NPFYFPKFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRC 416
              Y PKF Y A EVTYS    ++L ++L L+A G   Q YWDDG K WYPLY LPGDRC
Sbjct: 326 TAVYSPKFVYSADEVTYSIVTTSSLIVKLGLDAAGILHQMYWDDGRKDWYPLYTLPGDRC 385

Query: 417 DDYGLCGDFGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRI 476
           DDYGLCGDFGICTFSLT QC+CM GFEPKSP DW++ RWSDGCVR+D+QIC NGEGFKRI
Sbjct: 386 DDYGLCGDFGICTFSLTPQCNCMVGFEPKSPDDWKRFRWSDGCVRKDNQICRNGEGFKRI 445

Query: 477 SSVKLPDSSGYLVNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFV 536
            SVKLPDSSGYLVNVNTSI DCE ACLNNCSCLAYGIME+ TGGYGCV WFQKL+D RFV
Sbjct: 446 RSVKLPDSSGYLVNVNTSIDDCEVACLNNCSCLAYGIMELSTGGYGCVTWFQKLIDARFV 505

Query: 537 PENGQDLYVRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRGK----- 596
           PENGQD+YVRVAASELDS+N+K+++A+SVSVAS +GFLVLV+CFIL RRRK++GK     
Sbjct: 506 PENGQDIYVRVAASELDSSNRKVVIAVSVSVASLIGFLVLVVCFILWRRRKVKGKVQSQE 565

Query: 597 ------------------HFSSNTLIN-------LSGKLPCGQEIAVKRLAEGSGQGQSE 656
                             HFS +  I          GKLPCGQEIAVKRLAEGSGQGQSE
Sbjct: 566 NEVEMPLYDFTTIEIATNHFSFSNKIGEGGFGPVYKGKLPCGQEIAVKRLAEGSGQGQSE 625

Query: 657 FRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLNWKKRV 716
           F+NE+LLISQLQHRNLVKLLGFCIH EETLL+YEYMPNKSLD+FLFD E RSLLNW+KR+
Sbjct: 626 FKNEILLISQLQHRNLVKLLGFCIHHEETLLIYEYMPNKSLDYFLFDGEGRSLLNWQKRL 685

Query: 717 DIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQTMTKTQ 776
           DIIIG+ARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMF EDQTMTKTQ
Sbjct: 686 DIIIGIARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFPEDQTMTKTQ 745

Query: 777 RIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLGHAWKL 836
           R+VGTFGYMSPEY LDGCFSLKSD+FSFGVILLEIISG KNRGFFH DHQLNLLGHAWKL
Sbjct: 746 RVVGTFGYMSPEYALDGCFSLKSDVFSFGVILLEIISGKKNRGFFHTDHQLNLLGHAWKL 805

Query: 837 WDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESENMVLSH 896
           WD+GN LELMDA L+D+FQPSEALRCIQVGLLCVQQ P+ERP+MWSVLSMLESENM+LSH
Sbjct: 806 WDEGNPLELMDATLKDQFQPSEALRCIQVGLLCVQQDPNERPTMWSVLSMLESENMLLSH 865

Query: 897 PERPGFYTERMVLKTEKLSAEISTSNEVTVTLLHGR 903
           P+RPGFYTERMVLKT+K S +IS+SNEVTVTLLHGR
Sbjct: 866 PQRPGFYTERMVLKTDKSSTDISSSNEVTVTLLHGR 896

BLAST of MS025005 vs. NCBI nr
Match: XP_023519601.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1285.0 bits (3324), Expect = 0.0e+00
Identity = 625/818 (76.41%), Postives = 686/818 (83.86%), Query Frame = 0

Query: 125 KLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIFTP 184
           +LICR  LS   F WTT+ALF RKS A DSI  K GES+NGST+ILVSA QNFVLGIF P
Sbjct: 4   ELICRWKLSAFLFLWTTVALFPRKSLAIDSI--KTGESVNGSTQILVSAAQNFVLGIFNP 63

Query: 185 QGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWSSA 244
           QGSKF+YLGIW  YKNIPQTVVWVANRDNPLVN+S RL  N EG I LLNETGGVLWS+ 
Sbjct: 64  QGSKFQYLGIW--YKNIPQTVVWVANRDNPLVNASGRLTLNGEGKITLLNETGGVLWSTP 123

Query: 245 SAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKLTS 304
           S  TV++PVVQLL+TGNL+LRE GSENY+WQSFDYPSDTLLPGMKLGWD+KTG+NRKLTS
Sbjct: 124 SPGTVEQPVVQLLNTGNLVLRESGSENYLWQSFDYPSDTLLPGMKLGWDAKTGMNRKLTS 183

Query: 305 WESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFPKF 364
           W+S NDPSSGDF YSV ++GLPQ V+R+GP TT+R GPWYG  FSGS PLRE   YFPKF
Sbjct: 184 WKSTNDPSSGDFTYSVEMNGLPQFVVRRGPITTFRSGPWYGNGFSGSGPLRETAIYFPKF 243

Query: 365 EYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLCGD 424
            Y A E  YSYDAAN++++RLVLNA G F+QFYW + GKYWY LY LPGDRCD YG CGD
Sbjct: 244 NYNAHEAFYSYDAANDISVRLVLNAAGLFQQFYWVEDGKYWYTLYTLPGDRCDVYGFCGD 303

Query: 425 FGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLPDS 484
           FG+CTFSLTA+CDCMAGFEPKSP DWEK RWSDGCVRRD++ CGNGEGFKRIS+VKLPDS
Sbjct: 304 FGVCTFSLTAECDCMAGFEPKSPDDWEKFRWSDGCVRRDNRTCGNGEGFKRISNVKLPDS 363

Query: 485 SGYLVNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQDLY 544
           SGYLVNVNTSI DCEA CLNNCSCLAYGIME+ TGGYGCV WF KL DV+ V +NGQDLY
Sbjct: 364 SGYLVNVNTSIDDCEATCLNNCSCLAYGIMELPTGGYGCVTWFHKLRDVKSVLQNGQDLY 423

Query: 545 VRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRGKHFSSNTLIN---- 604
           VRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFIL RRRK+RG   S    +     
Sbjct: 424 VRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILERRRKVRGNMISPEITLGEIHS 483

Query: 605 ------------------------------------LSGKLPCGQEIAVKRLAEGSGQGQ 664
                                                 GKL CGQEIAVKRLAEGSGQG+
Sbjct: 484 QEKEVEMPIYDFRTIEIATNSFSFSNKIGEGGFGPVYKGKLSCGQEIAVKRLAEGSGQGK 543

Query: 665 SEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLNWKK 724
           SEFRNEVLLISQLQHRNLVKLLGFCIH++ETLLVYEYM NKSLD+FLFD++RR+ L W+K
Sbjct: 544 SEFRNEVLLISQLQHRNLVKLLGFCIHQQETLLVYEYMQNKSLDYFLFDDKRRASLKWQK 603

Query: 725 RVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQTMTK 784
           R+DIIIG+ARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMF EDQTMTK
Sbjct: 604 RLDIIIGIARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFEEDQTMTK 663

Query: 785 TQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLGHAW 844
           T+R+VGT+GYMSPEY LDGCFSLKSD+FSFGVI+LEI+SG KNRGFFH DHQLNLLGHAW
Sbjct: 664 TKRVVGTYGYMSPEYALDGCFSLKSDVFSFGVIVLEIVSGKKNRGFFHEDHQLNLLGHAW 723

Query: 845 KLWDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESENMVL 903
           KLW++GN LELMDAAL D FQ SEALRCIQVGLLCVQQS DERP+MWSVLSMLESE+M+L
Sbjct: 724 KLWNEGNGLELMDAALGDEFQGSEALRCIQVGLLCVQQSSDERPTMWSVLSMLESEDMLL 783

BLAST of MS025005 vs. ExPASy Swiss-Prot
Match: O81832 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 OS=Arabidopsis thaliana OX=3702 GN=At4g27290 PE=3 SV=4)

HSP 1 Score: 701.0 bits (1808), Expect = 1.7e-200
Identity = 363/776 (46.78%), Postives = 505/776 (65.08%), Query Frame = 0

Query: 142 IALFTRKSFASDSIHLKAGESI-NGSTRILVSAEQNFVLGIFTPQGSKFEYLGIWYNYKN 201
           I+LF+    A  +  L A +++ +G T  +VS   +F +G F+P GS+  YLGIWY   +
Sbjct: 12  ISLFSTILLAQATDILIANQTLKDGDT--IVSQGGSFEVGFFSPGGSRNRYLGIWYKKIS 71

Query: 202 IPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWSSASA-----KTVKEPVVQ 261
           + QTVVWVANRD+PL + S  LK +E G++ L N+   ++WSS+S+      +++ P+VQ
Sbjct: 72  L-QTVVWVANRDSPLYDLSGTLKVSENGSLCLFNDRNHIIWSSSSSPSSQKASLRNPIVQ 131

Query: 262 LLDTGNLMLREFG-SENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKLTSWESLNDPSSG 321
           +LDTGNL++R  G  ++Y+WQS DYP D  LPGMK G +  TGLNR LTSW +++DPS+G
Sbjct: 132 ILDTGNLVVRNSGDDQDYIWQSLDYPGDMFLPGMKYGLNFVTGLNRFLTSWRAIDDPSTG 191

Query: 322 DFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFPKFEYEAGEVTYS 381
           ++   +  +G+PQ  ++K     +R GPW G RF+G P L+ NP Y  ++ +   EV Y+
Sbjct: 192 NYTNKMDPNGVPQFFLKKNSVVVFRTGPWNGLRFTGMPNLKPNPIYRYEYVFTEEEVYYT 251

Query: 382 YDAAN-NLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLCGDFGICTFSLT 441
           Y   N ++  R+ LN +G  +++ W D  + W        D CD Y LCG +G C  + +
Sbjct: 252 YKLENPSVLTRMQLNPNGALQRYTWVDNLQSWNFYLSAMMDSCDQYTLCGSYGSCNINES 311

Query: 442 AQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGE-GFKRISSVKLPDSSGYLVNVN 501
             C C+ GF  K+PQ W    WS+GCVRR    CG GE GF +IS +KLPD+     + N
Sbjct: 312 PACRCLKGFVAKTPQAWVAGDWSEGCVRRVKLDCGKGEDGFLKISKLKLPDTRTSWYDKN 371

Query: 502 TSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQDLYVRVAASEL 561
             + +C+  CL NC+C AY   ++R GG GC+ WF  L+D+R   ENGQDLYVR+A+SE+
Sbjct: 372 MDLNECKKVCLRNCTCSAYSPFDIRDGGKGCILWFGDLIDIREYNENGQDLYVRLASSEI 431

Query: 562 DSTNKKLMVAISVSVAS---FLGFLVLVICFILRRRRKLRGKHFSSNTLINLSGKLPCGQ 621
           ++  ++     S         L FL L              K           G L CGQ
Sbjct: 432 ETLQRESSRVSSRKQEEEDLELPFLDLDTVSEATSGFSAGNKLGQGGFGPVYKGTLACGQ 491

Query: 622 EIAVKRLAEGSGQGQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDH 681
           E+AVKRL+  S QG  EF+NE+ LI++LQHRNLVK+LG+C+ +EE +L+YEY PNKSLD 
Sbjct: 492 EVAVKRLSRTSRQGVEEFKNEIKLIAKLQHRNLVKILGYCVDEEERMLIYEYQPNKSLDS 551

Query: 682 FLFDNERRSLLNWKKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISD 741
           F+FD ERR  L+W KRV+II G+ARG+LYLH DSRLRIIHRDLK SN+LLD++MN KISD
Sbjct: 552 FIFDKERRRELDWPKRVEIIKGIARGMLYLHEDSRLRIIHRDLKASNVLLDSDMNAKISD 611

Query: 742 FGMARMFGEDQTMTKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRG 801
           FG+AR  G D+T   T R+VGT+GYMSPEY +DG FSLKSD+FSFGV++LEI+SG +NRG
Sbjct: 612 FGLARTLGGDETEANTTRVVGTYGYMSPEYQIDGYFSLKSDVFSFGVLVLEIVSGRRNRG 671

Query: 802 FFHPDHQLNLLGHAWKLWDDGNALELMDAAL-EDRFQPSEALRCIQVGLLCVQQSPDERP 861
           F + +H+LNLLGHAW+ + +  A E++D A+ E     SE LR I +GLLCVQQ P +RP
Sbjct: 672 FRNEEHKLNLLGHAWRQFLEDKAYEIIDEAVNESCTDISEVLRVIHIGLLCVQQDPKDRP 731

Query: 862 SMWSVLSMLESENMVLSHPERPGFYTERMVL--KTEKLSAEISTSNEVTVTLLHGR 903
           +M  V+ ML SE M+L  P +PGF+ ER +L   T  ++ EI ++N  T++++  R
Sbjct: 732 NMSVVVLMLSSE-MLLLDPRQPGFFNERNLLFSDTVSINLEIPSNNFQTMSVIDPR 783

BLAST of MS025005 vs. ExPASy Swiss-Prot
Match: O81905 (Receptor-like serine/threonine-protein kinase SD1-8 OS=Arabidopsis thaliana OX=3702 GN=SD18 PE=1 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 6.7e-189
Identity = 357/842 (42.40%), Postives = 506/842 (60.10%), Query Frame = 0

Query: 136 FFFWTTIALFTRKSFASDSIHLKAGESIN-GSTRILVSAEQNFVLGIFTPQGSKFEYLGI 195
           FFF+  + LF   S ++++  L A ES+   S   +VS    F LG F P      YLGI
Sbjct: 13  FFFFFLLILFPAYSISANT--LSASESLTISSNNTIVSPGNVFELGFFKPGLDSRWYLGI 72

Query: 196 WYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS-SASAKTVKEPV 255
           WY   +  +T VWVANRD PL +S   LK + + N+V+L+++   +WS + +   V+ P+
Sbjct: 73  WYKAIS-KRTYVWVANRDTPLSSSIGTLKIS-DSNLVVLDQSDTPVWSTNLTGGDVRSPL 132

Query: 256 V-QLLDTGNLMLRE---FGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKLTSWESLN 315
           V +LLD GN +LR+      +  +WQSFD+P+DTLLP MKLGWD+KTG NR + SW+S +
Sbjct: 133 VAELLDNGNFVLRDSKNSAPDGVLWQSFDFPTDTLLPEMKLGWDAKTGFNRFIRSWKSPD 192

Query: 316 DPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFPKFEYEAG 375
           DPSSGDF++ +  +G P++ +    +  YR GPW G RFSG P ++   +    F     
Sbjct: 193 DPSSGDFSFKLETEGFPEIFLWNRESRMYRSGPWNGIRFSGVPEMQPFEYMVFNFTTSKE 252

Query: 376 EVTYSYD-AANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLCGDFGIC 435
           EVTYS+    +++  RL +++ G  ++F W +  + W   +  P D+CD+Y  CG +G C
Sbjct: 253 EVTYSFRITKSDVYSRLSISSSGLLQRFTWIETAQNWNQFWYAPKDQCDEYKECGVYGYC 312

Query: 436 TFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLPDSSGYL 495
             + +  C+C+ GF+P++PQ W     SDGCVR+    CG G+GF R+  +KLPD++   
Sbjct: 313 DSNTSPVCNCIKGFKPRNPQVWGLRDGSDGCVRKTLLSCGGGDGFVRLKKMKLPDTTTAS 372

Query: 496 VNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQDLYVRVA 555
           V+    +++CE  CL +C+C A+   ++R  G GCV W  +L D+R   + GQDLYVR+A
Sbjct: 373 VDRGIGVKECEQKCLRDCNCTAFANTDIRGSGSGCVTWTGELFDIRNYAKGGQDLYVRLA 432

Query: 556 ASEL-DSTNKKLMVAISVSVASFLGFLVLVICFILRRRRK-------------LRGKHFS 615
           A++L D  N+   +  S    S L  L  +I F+ +R++K             LR +   
Sbjct: 433 ATDLEDKRNRSAKIIGSSIGVSVLLLLSFIIFFLWKRKQKRSILIETPIVDHQLRSRDLL 492

Query: 616 SNTLI-------------------------------NLS---------------GKLPCG 675
            N ++                               N S               GKL  G
Sbjct: 493 MNEVVISSRRHISRENNTDDLELPLMEFEEVAMATNNFSNANKLGQGGFGIVYKGKLLDG 552

Query: 676 QEIAVKRLAEGSGQGQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLD 735
           QE+AVKRL++ S QG  EF+NEV LI++LQH NLV+LL  C+   E +L+YEY+ N SLD
Sbjct: 553 QEMAVKRLSKTSVQGTDEFKNEVKLIARLQHINLVRLLACCVDAGEKMLIYEYLENLSLD 612

Query: 736 HFLFDNERRSLLNWKKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKIS 795
             LFD  R S LNW+ R DII G+ARGLLYLH+DSR RIIHRDLK SNILLD  M PKIS
Sbjct: 613 SHLFDKSRNSKLNWQMRFDIINGIARGLLYLHQDSRFRIIHRDLKASNILLDKYMTPKIS 672

Query: 796 DFGMARMFGEDQTMTKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNR 855
           DFGMAR+FG D+T   T+++VGT+GYMSPEY +DG FS+KSD+FSFGV+LLEIIS  +N+
Sbjct: 673 DFGMARIFGRDETEANTRKVVGTYGYMSPEYAMDGIFSMKSDVFSFGVLLLEIISSKRNK 732

Query: 856 GFFHPDHQLNLLGHAWKLWDDGNALELMDAALED---RFQPSEALRCIQVGLLCVQQSPD 903
           GF++ D  LNLLG  W+ W +G  LE++D  + D    F+  E LRCIQ+GLLCVQ+  +
Sbjct: 733 GFYNSDRDLNLLGCVWRNWKEGKGLEIIDPIITDSSSTFRQHEILRCIQIGLLCVQERAE 792

BLAST of MS025005 vs. ExPASy Swiss-Prot
Match: Q39086 (Receptor-like serine/threonine-protein kinase SD1-7 OS=Arabidopsis thaliana OX=3702 GN=SD17 PE=1 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 4.5e-185
Identity = 357/839 (42.55%), Postives = 499/839 (59.48%), Query Frame = 0

Query: 136 FFFWTTIALFTRKSFASDSIHLKAGESIN-GSTRILVSAEQNFVLGIFTPQGSKFEYLGI 195
           FF +  + LF   S + ++  L A ES+   S + ++S  Q F LG F P  S   YLGI
Sbjct: 11  FFIFLILILFLAFSVSPNT--LSATESLTISSNKTIISPSQIFELGFFNPASSSRWYLGI 70

Query: 196 WYNYKNIP-QTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS-SASAKTVKEP 255
           W  YK IP +T VWVANRDNPL +S+  LK +   N+V+ +++   +WS + +   V+ P
Sbjct: 71  W--YKIIPIRTYVWVANRDNPLSSSNGTLKIS-GNNLVIFDQSDRPVWSTNITGGDVRSP 130

Query: 256 V-VQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKLTSWESLNDP 315
           V  +LLD GN +LR+  +   +WQSFD+P+DTLL  MKLGWD KTG NR L SW++ +DP
Sbjct: 131 VAAELLDNGNFLLRD-SNNRLLWQSFDFPTDTLLAEMKLGWDQKTGFNRILRSWKTTDDP 190

Query: 316 SSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFPKFEYEAGEV 375
           SSG+F+  +     P+  I    +  YR GPW G RFS  P   +  +    F     EV
Sbjct: 191 SSGEFSTKLETSEFPEFYICSKESILYRSGPWNGMRFSSVPGTIQVDYMVYNFTASKEEV 250

Query: 376 TYSYDA-ANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLCGDFGICTF 435
           TYSY     NL  RL LN+ G  ++  W +  + W  L+  P D CD+Y +CG+FG C  
Sbjct: 251 TYSYRINKTNLYSRLYLNSAGLLQRLTWFETTQSWKQLWYSPKDLCDNYKVCGNFGYCDS 310

Query: 436 SLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLPDSSGYLVN 495
           +    C C+ GF+P + Q W+    S GC+R+    C   +GF R+  +KLPD++  +V+
Sbjct: 311 NSLPNCYCIKGFKPVNEQAWDLRDGSAGCMRKTRLSCDGRDGFTRLKRMKLPDTTATIVD 370

Query: 496 VNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQDLYVRVAAS 555
               ++ C+  CL +C+C A+   ++R GG GCV W ++++D+R   + GQDLYVR+AA+
Sbjct: 371 REIGLKVCKERCLEDCNCTAFANADIRNGGSGCVIWTREILDMRNYAKGGQDLYVRLAAA 430

Query: 556 ELDS---TNKKLM-VAISVSVASFLGFLVL--------------------------VICF 615
           EL+     N+K++  +I VS+   L F++                           +I  
Sbjct: 431 ELEDKRIKNEKIIGSSIGVSILLLLSFVIFHFWKRKQKRSITIQTPNVDQVRSQDSLIND 490

Query: 616 ILRRRRKLRGKHFSSNTL-----------------------------INLSGKLPCGQEI 675
           ++  RR    K   S  L                             I   G+L  G+EI
Sbjct: 491 VVVSRRGYTSKEKKSEYLELPLLELEALATATNNFSNDNKLGQGGFGIVYKGRLLDGKEI 550

Query: 676 AVKRLAEGSGQGQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFL 735
           AVKRL++ S QG  EF NEV LI++LQH NLV+LLG C+ K E +L+YEY+ N SLD  L
Sbjct: 551 AVKRLSKMSSQGTDEFMNEVRLIAKLQHINLVRLLGCCVDKGEKMLIYEYLENLSLDSHL 610

Query: 736 FDNERRSLLNWKKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFG 795
           FD  R S LNW+KR DII G+ARGLLYLH+DSR RIIHRDLK SN+LLD  M PKISDFG
Sbjct: 611 FDQTRSSNLNWQKRFDIINGIARGLLYLHQDSRCRIIHRDLKASNVLLDKNMTPKISDFG 670

Query: 796 MARMFGEDQTMTKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFF 855
           MAR+FG ++T   T+R+VGT+GYMSPEY +DG FS+KSD+FSFGV+LLEIISG +N+GF+
Sbjct: 671 MARIFGREETEANTRRVVGTYGYMSPEYAMDGIFSMKSDVFSFGVLLLEIISGKRNKGFY 730

Query: 856 HPDHQLNLLGHAWKLWDDGNALELMDA----ALEDRFQPSEALRCIQVGLLCVQQSPDER 903
           + +  LNLLG  W+ W +GN LE++D     +L  +F   E LRCIQ+GLLCVQ+  ++R
Sbjct: 731 NSNRDLNLLGFVWRHWKEGNELEIVDPINIDSLSSKFPTHEILRCIQIGLLCVQERAEDR 790

BLAST of MS025005 vs. ExPASy Swiss-Prot
Match: O81833 (G-type lectin S-receptor-like serine/threonine-protein kinase SD1-1 OS=Arabidopsis thaliana OX=3702 GN=SD11 PE=1 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 5.5e-183
Identity = 346/783 (44.19%), Postives = 480/783 (61.30%), Query Frame = 0

Query: 170 LVSAEQNFVLGIFT---PQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNE 229
           L S +Q F LG F+    +  +  +LG+WY     P  VVWVANR+NPL  +S  L  + 
Sbjct: 40  LSSPDQVFQLGFFSLDQEEQPQHRFLGLWYME---PFAVVWVANRNNPLYGTSGFLNLSS 99

Query: 230 EGNIVLLNETGGVLWSSAS-----AKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPS 289
            G++ L +     LWSS+S     +KT   P++++  +GNL+  + G E  +WQSFDYP 
Sbjct: 100 LGDLQLFDGEHKALWSSSSSSTKASKTANNPLLKISCSGNLISSD-GEEAVLWQSFDYPM 159

Query: 290 DTLLPGMKLGWDSKTGLNRKLTSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNT--TYR 349
           +T+L GMKLG + KT +   L+SW++L DPS GDF  S+   GLPQL++RK  ++  +YR
Sbjct: 160 NTILAGMKLGKNFKTQMEWSLSSWKTLKDPSPGDFTLSLDTRGLPQLILRKNGDSSYSYR 219

Query: 350 GGPWYGTRFSGSPPL-RENPFYFPKFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYW 409
            G W G  F+G+P + REN  +  KF   A EV YS+   + +  RLVLN  G   +F  
Sbjct: 220 LGSWNGLSFTGAPAMGRENSLFDYKFTSSAQEVNYSWTPRHRIVSRLVLNNTGKLHRFIQ 279

Query: 410 DDGGKYWYPLYILPGDRCDDYGLCGDFGICTFSL--TAQCDCMAGFEPKSPQDWEKLRWS 469
               + W      P D CD Y +CG + +C  +   T  C C+ GF+PKS + W   R +
Sbjct: 280 SKQNQ-WILANTAPEDECDYYSICGAYAVCGINSKNTPSCSCLQGFKPKSGRKWNISRGA 339

Query: 470 DGCVRRDSQICGNGEGFKRISSVKLPDS--SGYLVNVNTSIQDCEAACLNNCSCLAYGIM 529
            GCV      C   + F +   +KLPD+  S Y      +++DC+  C +NCSC AY   
Sbjct: 340 YGCVHEIPTNCEKKDAFVKFPGLKLPDTSWSWYDAKNEMTLEDCKIKCSSNCSCTAYANT 399

Query: 530 EVRTGGYGCVNWFQKLMDVRFVPENGQDLYVRVAASELDSTNKKLMVAISVSVASFLGFL 589
           ++R GG GC+ WF  L+D+R     GQD+Y+R+  ++++   ++++  +  SV +    L
Sbjct: 400 DIREGGKGCLLWFGDLVDMREYSSFGQDVYIRMGFAKIEFKGREVVGMVVGSVVAIAVVL 459

Query: 590 VLVI-CFILRRRRKLRGKHFSSN-----------------------TLINL--------- 649
           V+V  CF  +  ++ RG++F                          + +N          
Sbjct: 460 VVVFACFRKKIMKRYRGENFRKGIEEEDLDLPIFDRKTISIATDDFSYVNFLGRGGFGPV 519

Query: 650 -SGKLPCGQEIAVKRLAEGSGQGQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYE 709
             GKL  GQEIAVKRL+  SGQG  EF+NEV LI++LQHRNLV+LLG CI  EE +L+YE
Sbjct: 520 YKGKLEDGQEIAVKRLSANSGQGVEEFKNEVKLIAKLQHRNLVRLLGCCIQGEECMLIYE 579

Query: 710 YMPNKSLDHFLFDNERRSLLNWKKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLD 769
           YMPNKSLD F+FD  R + L+WKKR++II GVARG+LYLH+DSRLRIIHRDLK  N+LLD
Sbjct: 580 YMPNKSLDFFIFDERRSTELDWKKRMNIINGVARGILYLHQDSRLRIIHRDLKAGNVLLD 639

Query: 770 NEMNPKISDFGMARMFGEDQTMTKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLE 829
           N+MNPKISDFG+A+ FG DQ+ + T R+VGT+GYM PEY +DG FS+KSD+FSFGV++LE
Sbjct: 640 NDMNPKISDFGLAKSFGGDQSESSTNRVVGTYGYMPPEYAIDGHFSVKSDVFSFGVLVLE 699

Query: 830 IISGMKNRGFFHPDHQLNLLGHAWKLWDDGNALELMDAA-LEDRFQPSEALRCIQVGLLC 889
           II+G  NRGF H DH LNLLGH WK+W +   +E+ +   LE+     E LRCI V LLC
Sbjct: 700 IITGKTNRGFRHADHDLNLLGHVWKMWVEDREIEVPEEEWLEETSVIPEVLRCIHVALLC 759

Query: 890 VQQSPDERPSMWSVLSMLESENMVLSHPERPGFYTERMVLKTEKLSAEISTSNEVTVTLL 903
           VQQ P++RP+M SV+ M  S++  L HP +PGF+T R V      S  + + NEV++T+L
Sbjct: 760 VQQKPEDRPTMASVVLMFGSDSS-LPHPTQPGFFTNRNVPDISS-SLSLRSQNEVSITML 815

BLAST of MS025005 vs. ExPASy Swiss-Prot
Match: Q9S972 (Receptor-like serine/threonine-protein kinase SD1-6 OS=Arabidopsis thaliana OX=3702 GN=SD16 PE=1 SV=2)

HSP 1 Score: 632.1 bits (1629), Expect = 9.6e-180
Identity = 355/844 (42.06%), Postives = 492/844 (58.29%), Query Frame = 0

Query: 136 FFFWTTIALFTRKSFASDSIHLKAGESIN-GSTRILVSAEQNFVLGIFTPQGSKFEYLGI 195
           +F    I LF   +F+  + +  A ES+   S + ++S  Q F LG F P  S   YLGI
Sbjct: 11  YFILFIIILFL--AFSVYASNFSATESLTISSNKTIISPSQIFELGFFNPDSSSRWYLGI 70

Query: 196 WYNYKNIP-QTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS-SASAKTVKEP 255
           W  YK IP +T VWVANRDNPL +S+  LK + + N+V+ +++   +WS + +   V+ P
Sbjct: 71  W--YKIIPIRTYVWVANRDNPLSSSNGTLKIS-DNNLVIFDQSDRPVWSTNITGGDVRSP 130

Query: 256 V-VQLLDTGNLMLREFGS---ENYVWQSFDYPSDTLLPGMKLGWDSKT-GLNRKLTSWES 315
           V  +LLD GN +LR+  +     ++WQSFD+P+DTLL  MK+GWD+K+ G NR L SW++
Sbjct: 131 VAAELLDYGNFVLRDSKNNKPSGFLWQSFDFPTDTLLSDMKMGWDNKSGGFNRILRSWKT 190

Query: 316 LNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFPKFEYE 375
            +DPSSGDF+  +   G P+  I    + TYR GPW G RFS  P ++   +    F   
Sbjct: 191 TDDPSSGDFSTKLRTSGFPEFYIYNKESITYRSGPWLGNRFSSVPGMKPVDYIDNSFTEN 250

Query: 376 AGEVTYSYDA-ANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLCGDFG 435
             +V YSY     N+   L L++ G  ++  W +  + W  L+  P D CD+Y  CG++G
Sbjct: 251 NQQVVYSYRVNKTNIYSILSLSSTGLLQRLTWMEAAQSWKQLWYSPKDLCDNYKECGNYG 310

Query: 436 ICTFSLTAQCDCMAGFEPKSPQDWEKLR-WSDGCVRRDSQICGNGEGFKRISSVKLPDSS 495
            C  + +  C+C+ GFEP + Q    LR  S GCVR+    C   +GF R+  ++LPD++
Sbjct: 311 YCDANTSPICNCIKGFEPMNEQ--AALRDDSVGCVRKTKLSCDGRDGFVRLKKMRLPDTT 370

Query: 496 GYLVNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQDLYV 555
              V+    +++CE  CL  C+C A+   ++R GG GCV W   L D+R   + GQDLYV
Sbjct: 371 ETSVDKGIGLKECEERCLKGCNCTAFANTDIRNGGSGCVIWSGGLFDIRNYAKGGQDLYV 430

Query: 556 RVAASELDSTNKKLMVAISVSV-ASFLGFLVLVICFILRRRRK------------LRGKH 615
           RVAA +L+    K    I  S+  S L  L  +I    +R++K            +R + 
Sbjct: 431 RVAAGDLEDKRIKSKKIIGSSIGVSILLLLSFIIFHFWKRKQKRSITIQTPIVDLVRSQD 490

Query: 616 FSSNTLINLS----------------------------------------------GKLP 675
              N L+  S                                              G L 
Sbjct: 491 SLMNELVKASRSYTSKENKTDYLELPLMEWKALAMATNNFSTDNKLGQGGFGIVYKGMLL 550

Query: 676 CGQEIAVKRLAEGSGQGQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKS 735
            G+EIAVKRL++ S QG  EF NEV LI++LQH NLV+LLG C+ K E +L+YEY+ N S
Sbjct: 551 DGKEIAVKRLSKMSSQGTDEFMNEVRLIAKLQHINLVRLLGCCVDKGEKMLIYEYLENLS 610

Query: 736 LDHFLFDNERRSLLNWKKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPK 795
           LD  LFD  R S LNW+KR DII G+ARGLLYLH+DSR RIIHRDLK SN+LLD  M PK
Sbjct: 611 LDSHLFDQTRSSNLNWQKRFDIINGIARGLLYLHQDSRCRIIHRDLKASNVLLDKNMTPK 670

Query: 796 ISDFGMARMFGEDQTMTKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMK 855
           ISDFGMAR+FG ++T   T+R+VGT+GYMSPEY +DG FS+KSD+FSFGV+LLEIISG +
Sbjct: 671 ISDFGMARIFGREETEANTRRVVGTYGYMSPEYAMDGIFSMKSDVFSFGVLLLEIISGKR 730

Query: 856 NRGFFHPDHQLNLLGHAWKLWDDGNALELMDA----ALEDRFQPSEALRCIQVGLLCVQQ 903
           N+GF++ +  LNLLG  W+ W +G  LE++D     AL   F   E LRCIQ+GLLCVQ+
Sbjct: 731 NKGFYNSNRDLNLLGFVWRHWKEGKELEIVDPINIDALSSEFPTHEILRCIQIGLLCVQE 790

BLAST of MS025005 vs. ExPASy TrEMBL
Match: A0A6J1CE24 (Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=LOC111010698 PE=3 SV=1)

HSP 1 Score: 1568.9 bits (4061), Expect = 0.0e+00
Identity = 770/816 (94.36%), Postives = 772/816 (94.61%), Query Frame = 0

Query: 123 MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF 182
           MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF
Sbjct: 1   MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF 60

Query: 183 TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS 242
           TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS
Sbjct: 61  TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS 120

Query: 243 SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL 302
           SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL
Sbjct: 121 SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL 180

Query: 303 TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP 362
           TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP
Sbjct: 181 TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP 240

Query: 363 KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC 422
           KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC
Sbjct: 241 KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC 300

Query: 423 GDFGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLP 482
           GDFGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLP
Sbjct: 301 GDFGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLP 360

Query: 483 DSSGYLVNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQD 542
           DSSGYLVNVN SIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQD
Sbjct: 361 DSSGYLVNVNKSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQD 420

Query: 543 LYVRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRG------------ 602
           LYVRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRG            
Sbjct: 421 LYVRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRGNEITASEIQSQE 480

Query: 603 -----------------KHFSSNTLIN-------LSGKLPCGQEIAVKRLAEGSGQGQSE 662
                             +FS +  I          GKLPCGQEIAVKRLAEGSGQGQSE
Sbjct: 481 NEVEMPLYDFTTIETATNYFSFSNKIGEGGFGPVYKGKLPCGQEIAVKRLAEGSGQGQSE 540

Query: 663 FRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLNWKKRV 722
           FRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLNWKKRV
Sbjct: 541 FRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLNWKKRV 600

Query: 723 DIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQTMTKTQ 782
           DIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQTMTKTQ
Sbjct: 601 DIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQTMTKTQ 660

Query: 783 RIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLGHAWKL 842
           RIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLGHAWKL
Sbjct: 661 RIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLGHAWKL 720

Query: 843 WDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESENMVLSH 902
           WDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESENMVLSH
Sbjct: 721 WDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESENMVLSH 780

BLAST of MS025005 vs. ExPASy TrEMBL
Match: A0A6J1CDK0 (Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=LOC111010698 PE=3 SV=1)

HSP 1 Score: 1455.3 bits (3766), Expect = 0.0e+00
Identity = 705/820 (85.98%), Postives = 746/820 (90.98%), Query Frame = 0

Query: 123 MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF 182
           MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF
Sbjct: 1   MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF 60

Query: 183 TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS 242
           TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS
Sbjct: 61  TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS 120

Query: 243 SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL 302
           SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL
Sbjct: 121 SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL 180

Query: 303 TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP 362
           TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP
Sbjct: 181 TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP 240

Query: 363 KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC 422
           KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC
Sbjct: 241 KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC 300

Query: 423 GDFGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLP 482
           GDFGICTFSLTAQCDCMAGFEPKSP+ WE+  WSDGCVRRD +IC NGEGFKRI SVKLP
Sbjct: 301 GDFGICTFSLTAQCDCMAGFEPKSPESWERFMWSDGCVRRDIRICRNGEGFKRIRSVKLP 360

Query: 483 DSSGYLVNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQD 542
           DSSG+LVNVNTSIQDC+AACLNNCSCLAYGIME+ TGGYGCV WFQKL+DVRFV +NGQD
Sbjct: 361 DSSGHLVNVNTSIQDCKAACLNNCSCLAYGIMELSTGGYGCVTWFQKLVDVRFVLQNGQD 420

Query: 543 LYVRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRGKHFSSN------ 602
           LYVRVAASELDST KKL+VAISVSV SFLGF VLVIC +LRRR+K RG   +        
Sbjct: 421 LYVRVAASELDSTKKKLIVAISVSVVSFLGFSVLVICIVLRRRKKARGNEIAPEITAGEF 480

Query: 603 --------------TLINLS--------------------GKLPCGQEIAVKRLAEGSGQ 662
                         T+I  +                    GKLPCGQEIAVKRLAEGSGQ
Sbjct: 481 QSQENEVEMPIYDFTMIETATNYFSFANKIGEGGFGPVYKGKLPCGQEIAVKRLAEGSGQ 540

Query: 663 GQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLNW 722
           GQSEF+NEVLLISQLQHRNLVKLLGFCIH+EETLLVYEYMPNKSLD+FLFDN+RRSLL+W
Sbjct: 541 GQSEFKNEVLLISQLQHRNLVKLLGFCIHQEETLLVYEYMPNKSLDYFLFDNKRRSLLDW 600

Query: 723 KKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQTM 782
           KKR+DIIIG+ARGLLYLHRDSRLRIIHRDLKVSNILLD+EMNPKISDFGMARMF ED+TM
Sbjct: 601 KKRLDIIIGIARGLLYLHRDSRLRIIHRDLKVSNILLDDEMNPKISDFGMARMFAEDETM 660

Query: 783 TKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLGH 842
           TKT+R+VGT+GYMSPEY LDG FSLKSD+FSFGVILLEI+SG KNRGFFH +HQLNLLGH
Sbjct: 661 TKTKRVVGTYGYMSPEYALDGYFSLKSDVFSFGVILLEIVSGKKNRGFFHSEHQLNLLGH 720

Query: 843 AWKLWDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESENM 902
           AWKLWD+ NALELMDAAL+D+FQPSEA+RCIQVGLLCVQQ+PDERP+MWSV+SMLESENM
Sbjct: 721 AWKLWDEENALELMDAALKDQFQPSEAVRCIQVGLLCVQQNPDERPTMWSVISMLESENM 780

BLAST of MS025005 vs. ExPASy TrEMBL
Match: A0A6J1CF73 (Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=LOC111010698 PE=3 SV=1)

HSP 1 Score: 1291.2 bits (3340), Expect = 0.0e+00
Identity = 633/821 (77.10%), Postives = 703/821 (85.63%), Query Frame = 0

Query: 123 MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF 182
           M KLICRC LS   FF TTIALF RKSFASD I  KAGES+N ST+ILVS+ QNFVLG+F
Sbjct: 1   MSKLICRCKLSAFLFFLTTIALFPRKSFASDII--KAGESLNVSTQILVSSRQNFVLGMF 60

Query: 183 TPQGSKFEYLGIWYNYKN-IPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLW 242
           +PQGSKF+YLGIW  YKN IPQTVVWVANRDNP+VNSSARL F ++GNIVLLNETGGVLW
Sbjct: 61  SPQGSKFQYLGIW--YKNIIPQTVVWVANRDNPIVNSSARLTF-KDGNIVLLNETGGVLW 120

Query: 243 SSASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRK 302
           SS S  +VKEPV QLLDTGNL+LR+ GSENYVWQSFDYP DTLLPGMKLGWDSKTGLNRK
Sbjct: 121 SSTS--SVKEPVAQLLDTGNLVLRDSGSENYVWQSFDYPFDTLLPGMKLGWDSKTGLNRK 180

Query: 303 LTSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYF 362
           LTSW+S NDPSSGDF YS+ ++GLPQ +IRKG  TT+RGGPWYGTRFSG+ PLR+   Y 
Sbjct: 181 LTSWKSSNDPSSGDFTYSLDVNGLPQFLIRKGSITTFRGGPWYGTRFSGTAPLRDTAIYS 240

Query: 363 PKFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGL 422
           PKF+Y A E  +SYDA NNL +RL LN+ G+F+QFYWDDGGK+WY +Y +PGDRCDDYGL
Sbjct: 241 PKFDYRADEAFFSYDAVNNLIVRLALNSAGSFQQFYWDDGGKFWYSMYAMPGDRCDDYGL 300

Query: 423 CGDFGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKL 482
           CGDFGICTFSLTAQCDCMAGFEPKSP+ WE+  WSDGCVRRD +IC NGEGFKRI SVKL
Sbjct: 301 CGDFGICTFSLTAQCDCMAGFEPKSPESWERFMWSDGCVRRDIRICRNGEGFKRIRSVKL 360

Query: 483 PDSSGYLVNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQ 542
           PDSSG+LVNVNTSIQDC+AACLNNCSCLAYGIME+ TGGYGCV WFQKL+DVRFV +NGQ
Sbjct: 361 PDSSGHLVNVNTSIQDCKAACLNNCSCLAYGIMELSTGGYGCVTWFQKLVDVRFVLQNGQ 420

Query: 543 DLYVRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRGKHFSSN----- 602
           DLYVRVAASELDST KKL+VAISVSV SFLGF VLVIC +LRRR+K RG   +       
Sbjct: 421 DLYVRVAASELDSTKKKLIVAISVSVVSFLGFSVLVICIVLRRRKKARGNEIAPEITAGE 480

Query: 603 ---------------TLINLS--------------------GKLPCGQEIAVKRLAEGSG 662
                          T+I  +                    GKLPCGQEIAVKRLAEGSG
Sbjct: 481 FQSQENEVEMPIYDFTMIETATNYFSFANKIGEGGFGPVYKGKLPCGQEIAVKRLAEGSG 540

Query: 663 QGQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLN 722
           QGQSEF+NEVLLISQLQHRNLVKLLGFCIH+EETLLVYEYMPNKSLD+FLFDN+RRSLL+
Sbjct: 541 QGQSEFKNEVLLISQLQHRNLVKLLGFCIHQEETLLVYEYMPNKSLDYFLFDNKRRSLLD 600

Query: 723 WKKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQT 782
           WKKR+DIIIG+ARGLLYLHRDSRLRIIHRDLKVSNILLD+EMNPKISDFGMARMF ED+T
Sbjct: 601 WKKRLDIIIGIARGLLYLHRDSRLRIIHRDLKVSNILLDDEMNPKISDFGMARMFAEDET 660

Query: 783 MTKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLG 842
           MTKT+R+VGT+GYMSPEY LDG FSLKSD+FSFGVILLEI+SG KNRGFFH +HQLNLLG
Sbjct: 661 MTKTKRVVGTYGYMSPEYALDGYFSLKSDVFSFGVILLEIVSGKKNRGFFHSEHQLNLLG 720

Query: 843 HAWKLWDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESEN 902
           HAWKLWD+ NALELMDAAL+D+FQPSEA+RCIQVGLLCVQQ+PDERP+MWSV+SMLESEN
Sbjct: 721 HAWKLWDEENALELMDAALKDQFQPSEAVRCIQVGLLCVQQNPDERPTMWSVISMLESEN 780

BLAST of MS025005 vs. ExPASy TrEMBL
Match: A0A6J1EHE9 (Receptor-like serine/threonine-protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111434317 PE=3 SV=1)

HSP 1 Score: 1282.3 bits (3317), Expect = 0.0e+00
Identity = 628/819 (76.68%), Postives = 685/819 (83.64%), Query Frame = 0

Query: 125 KLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIFTP 184
           +LICR  LS   F WTT+ALF RKS A DSI  K GE +NGST+ILVSA QNFVLGIF P
Sbjct: 4   ELICRWKLSAFLFLWTTVALFPRKSLAIDSI--KTGELVNGSTQILVSAAQNFVLGIFNP 63

Query: 185 QGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWSSA 244
           QGSKF+YLGIW  YKNIPQTVVWVANRDNPLVNSS RL  N EG I LLNETGGVLWS+ 
Sbjct: 64  QGSKFQYLGIW--YKNIPQTVVWVANRDNPLVNSSGRLALNGEGKITLLNETGGVLWSTP 123

Query: 245 SAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKLTS 304
           S  TV++PVVQLL+TGNL+LRE GSENY+WQSFDYPSDTLLPGMKLGWD+KTG+NRKLTS
Sbjct: 124 SPGTVEQPVVQLLNTGNLVLRESGSENYLWQSFDYPSDTLLPGMKLGWDAKTGMNRKLTS 183

Query: 305 WESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFPKF 364
           W+S NDPSSGDF YSV ++GLPQ V+R+GP TT+R GPWYG  FSGS PLRE   YFPKF
Sbjct: 184 WKSTNDPSSGDFTYSVEMNGLPQFVVRRGPITTFRSGPWYGNGFSGSGPLRETAIYFPKF 243

Query: 365 EYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLCGD 424
            Y A E  YSYDAAN++++RLVLNA G F+QFYW D G YWY LY LPGDRCD YG CGD
Sbjct: 244 TYNAHEAFYSYDAANDISVRLVLNAAGLFQQFYWVDDGNYWYTLYTLPGDRCDVYGFCGD 303

Query: 425 FGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLPDS 484
           FG+CTFSLTA+CDCMAGFEPKSP DWEK RWSDGCVRRD++ CGNGEGFKRIS+VKLPDS
Sbjct: 304 FGVCTFSLTAECDCMAGFEPKSPDDWEKFRWSDGCVRRDNRTCGNGEGFKRISNVKLPDS 363

Query: 485 SGYLVNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQDLY 544
           SGYLVNVNTSI DCEA CLNNCSCLAYGIME+ TGGYGCV WF KL DV+FV +NGQDLY
Sbjct: 364 SGYLVNVNTSIDDCEATCLNNCSCLAYGIMELPTGGYGCVTWFHKLRDVKFVLQNGQDLY 423

Query: 545 VRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRG-------------- 604
           VRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFIL RRRK+RG              
Sbjct: 424 VRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILGRRRKVRGGNMISPEITLGKIH 483

Query: 605 --------------------KHFSSNTLIN-------LSGKLPCGQEIAVKRLAEGSGQG 664
                                 FS +  I          GKL CGQEIAVKRLAEGSGQG
Sbjct: 484 SQEKEVEMPIYDFRTIEIATNSFSLSNKIGEGGFGPVYKGKLSCGQEIAVKRLAEGSGQG 543

Query: 665 QSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLNWK 724
           +SEFRNEVLLISQLQHRNLVKLLGFCIH++ETLLVYEYM NKSLD+FLFD++RR+ L W+
Sbjct: 544 KSEFRNEVLLISQLQHRNLVKLLGFCIHQQETLLVYEYMQNKSLDYFLFDDKRRASLKWQ 603

Query: 725 KRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQTMT 784
           KR+DIIIG+ARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMF EDQTMT
Sbjct: 604 KRLDIIIGIARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFEEDQTMT 663

Query: 785 KTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLGHA 844
           KT+R+VGT+GYMSPEY LDGCFSLKSD+FSFGVI+LEI+SG KNRGFFH DH LNLLGHA
Sbjct: 664 KTKRVVGTYGYMSPEYALDGCFSLKSDVFSFGVIVLEIVSGKKNRGFFHEDHHLNLLGHA 723

Query: 845 WKLWDDGNALELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESENMV 903
           WKLW+ GN LELMDAAL D FQ SEALRCIQVGLLCVQQS DERP+MWSVLSMLESE+M+
Sbjct: 724 WKLWNGGNGLELMDAALGDEFQGSEALRCIQVGLLCVQQSSDERPTMWSVLSMLESEDML 783

BLAST of MS025005 vs. ExPASy TrEMBL
Match: A0A1S4DSS7 (Receptor-like serine/threonine-protein kinase OS=Cucumis melo OX=3656 GN=LOC103489234 PE=3 SV=1)

HSP 1 Score: 1279.2 bits (3309), Expect = 0.0e+00
Identity = 623/811 (76.82%), Postives = 689/811 (84.96%), Query Frame = 0

Query: 123 MGKLICRCNLSGLFFFWTTIALFTRKSFASDSIHLKAGESINGSTRILVSAEQNFVLGIF 182
           MG+LI +C LS   F  TTIALF  KS   DSI  KAGESINGST+ILVSA++NFVLGIF
Sbjct: 1   MGELISKCKLSAFLFLLTTIALFPTKSHGKDSI--KAGESINGSTQILVSAKKNFVLGIF 60

Query: 183 TPQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS 242
           TPQGSKF+YLGIW+N  NIPQT+VWVANRDNP+VNS+ RL+F   GNIVLLNE  G+LWS
Sbjct: 61  TPQGSKFQYLGIWFN--NIPQTIVWVANRDNPIVNSTGRLEF-RRGNIVLLNEADGILWS 120

Query: 243 SASAKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKL 302
           S S  T K+PV QLLDTGN ++RE GSE+YVWQSFDYPSDTLLPGMKLGW S TGLNRKL
Sbjct: 121 SISLGTTKDPVAQLLDTGNWVVRESGSEDYVWQSFDYPSDTLLPGMKLGWSSNTGLNRKL 180

Query: 303 TSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFP 362
            SW+SLNDPS GDF YSV L+GLPQLV RKGP  TYRGGPWYG RFSGS PLR+   Y P
Sbjct: 181 RSWKSLNDPSFGDFTYSVDLNGLPQLVTRKGPIITYRGGPWYGNRFSGSAPLRDTAVYSP 240

Query: 363 KFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLC 422
           KFEY A EVTYS    ++L ++L L+A G   Q YWDDG K WYPLY LPGDRCDDYGLC
Sbjct: 241 KFEYSADEVTYSIVTTSSLIVKLGLDAAGILHQTYWDDGRKDWYPLYTLPGDRCDDYGLC 300

Query: 423 GDFGICTFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLP 482
           G+FGICTFSLT QC+CM GFEPKSP DW + RWSDGCVR+D+QICGNGEGFKRI SVKLP
Sbjct: 301 GNFGICTFSLTPQCNCMVGFEPKSPDDWRRFRWSDGCVRKDNQICGNGEGFKRIRSVKLP 360

Query: 483 DSSGYLVNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQD 542
           DSSGYLVNVNTSI DCEAACLNNCSC AYGIME+  GGYGCV WF+KL+D RFVPENGQD
Sbjct: 361 DSSGYLVNVNTSINDCEAACLNNCSCFAYGIMELSAGGYGCVTWFEKLIDARFVPENGQD 420

Query: 543 LYVRVAASELDSTNKKLMVAISVSVASFLGFLVLVICFILRRRRKLRGK----------- 602
           +YVRVAASELDS+N+K+++A+SVSVAS +GFLVLV+C IL RRRK++G+           
Sbjct: 421 IYVRVAASELDSSNRKVVLAVSVSVASLVGFLVLVVCLILWRRRKVKGEVQSLENEVEMP 480

Query: 603 ------------HFSSNTLIN-------LSGKLPCGQEIAVKRLAEGSGQGQSEFRNEVL 662
                       HFS +  I          GKLPCGQEIAVKRLAEGSGQGQSEF+NE+L
Sbjct: 481 LYDFTTIEIATNHFSFSNKIGEGGFGPVYKGKLPCGQEIAVKRLAEGSGQGQSEFKNEIL 540

Query: 663 LISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFLFDNERRSLLNWKKRVDIIIGV 722
           LISQLQHRNLVKLLGFCIH EETLLVYEYMPNKSLD+FLFD+ERRSLLNW+KR+DIIIG+
Sbjct: 541 LISQLQHRNLVKLLGFCIHHEETLLVYEYMPNKSLDYFLFDDERRSLLNWQKRLDIIIGI 600

Query: 723 ARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFGEDQTMTKTQRIVGTF 782
           ARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMF EDQTMTKTQR+VGTF
Sbjct: 601 ARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFGMARMFPEDQTMTKTQRVVGTF 660

Query: 783 GYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFFHPDHQLNLLGHAWKLWDDGNA 842
           GYMSPEY LDGCFSLKSD+FSFGVILLEIISG KNRGFFH DHQLNLLGHAWKLWD+GNA
Sbjct: 661 GYMSPEYALDGCFSLKSDVFSFGVILLEIISGKKNRGFFHTDHQLNLLGHAWKLWDEGNA 720

Query: 843 LELMDAALEDRFQPSEALRCIQVGLLCVQQSPDERPSMWSVLSMLESENMVLSHPERPGF 902
           LELMDA L+D+FQPSEALRCIQVGLLCVQQ P+ERP+MWSVL+MLESEN +LSHP+RPGF
Sbjct: 721 LELMDAKLKDQFQPSEALRCIQVGLLCVQQDPNERPTMWSVLTMLESENTLLSHPQRPGF 780

BLAST of MS025005 vs. TAIR 10
Match: AT4G27290.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 701.0 bits (1808), Expect = 1.2e-201
Identity = 363/776 (46.78%), Postives = 505/776 (65.08%), Query Frame = 0

Query: 142 IALFTRKSFASDSIHLKAGESI-NGSTRILVSAEQNFVLGIFTPQGSKFEYLGIWYNYKN 201
           I+LF+    A  +  L A +++ +G T  +VS   +F +G F+P GS+  YLGIWY   +
Sbjct: 12  ISLFSTILLAQATDILIANQTLKDGDT--IVSQGGSFEVGFFSPGGSRNRYLGIWYKKIS 71

Query: 202 IPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWSSASA-----KTVKEPVVQ 261
           + QTVVWVANRD+PL + S  LK +E G++ L N+   ++WSS+S+      +++ P+VQ
Sbjct: 72  L-QTVVWVANRDSPLYDLSGTLKVSENGSLCLFNDRNHIIWSSSSSPSSQKASLRNPIVQ 131

Query: 262 LLDTGNLMLREFG-SENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKLTSWESLNDPSSG 321
           +LDTGNL++R  G  ++Y+WQS DYP D  LPGMK G +  TGLNR LTSW +++DPS+G
Sbjct: 132 ILDTGNLVVRNSGDDQDYIWQSLDYPGDMFLPGMKYGLNFVTGLNRFLTSWRAIDDPSTG 191

Query: 322 DFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFPKFEYEAGEVTYS 381
           ++   +  +G+PQ  ++K     +R GPW G RF+G P L+ NP Y  ++ +   EV Y+
Sbjct: 192 NYTNKMDPNGVPQFFLKKNSVVVFRTGPWNGLRFTGMPNLKPNPIYRYEYVFTEEEVYYT 251

Query: 382 YDAAN-NLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLCGDFGICTFSLT 441
           Y   N ++  R+ LN +G  +++ W D  + W        D CD Y LCG +G C  + +
Sbjct: 252 YKLENPSVLTRMQLNPNGALQRYTWVDNLQSWNFYLSAMMDSCDQYTLCGSYGSCNINES 311

Query: 442 AQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGE-GFKRISSVKLPDSSGYLVNVN 501
             C C+ GF  K+PQ W    WS+GCVRR    CG GE GF +IS +KLPD+     + N
Sbjct: 312 PACRCLKGFVAKTPQAWVAGDWSEGCVRRVKLDCGKGEDGFLKISKLKLPDTRTSWYDKN 371

Query: 502 TSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQDLYVRVAASEL 561
             + +C+  CL NC+C AY   ++R GG GC+ WF  L+D+R   ENGQDLYVR+A+SE+
Sbjct: 372 MDLNECKKVCLRNCTCSAYSPFDIRDGGKGCILWFGDLIDIREYNENGQDLYVRLASSEI 431

Query: 562 DSTNKKLMVAISVSVAS---FLGFLVLVICFILRRRRKLRGKHFSSNTLINLSGKLPCGQ 621
           ++  ++     S         L FL L              K           G L CGQ
Sbjct: 432 ETLQRESSRVSSRKQEEEDLELPFLDLDTVSEATSGFSAGNKLGQGGFGPVYKGTLACGQ 491

Query: 622 EIAVKRLAEGSGQGQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDH 681
           E+AVKRL+  S QG  EF+NE+ LI++LQHRNLVK+LG+C+ +EE +L+YEY PNKSLD 
Sbjct: 492 EVAVKRLSRTSRQGVEEFKNEIKLIAKLQHRNLVKILGYCVDEEERMLIYEYQPNKSLDS 551

Query: 682 FLFDNERRSLLNWKKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISD 741
           F+FD ERR  L+W KRV+II G+ARG+LYLH DSRLRIIHRDLK SN+LLD++MN KISD
Sbjct: 552 FIFDKERRRELDWPKRVEIIKGIARGMLYLHEDSRLRIIHRDLKASNVLLDSDMNAKISD 611

Query: 742 FGMARMFGEDQTMTKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRG 801
           FG+AR  G D+T   T R+VGT+GYMSPEY +DG FSLKSD+FSFGV++LEI+SG +NRG
Sbjct: 612 FGLARTLGGDETEANTTRVVGTYGYMSPEYQIDGYFSLKSDVFSFGVLVLEIVSGRRNRG 671

Query: 802 FFHPDHQLNLLGHAWKLWDDGNALELMDAAL-EDRFQPSEALRCIQVGLLCVQQSPDERP 861
           F + +H+LNLLGHAW+ + +  A E++D A+ E     SE LR I +GLLCVQQ P +RP
Sbjct: 672 FRNEEHKLNLLGHAWRQFLEDKAYEIIDEAVNESCTDISEVLRVIHIGLLCVQQDPKDRP 731

Query: 862 SMWSVLSMLESENMVLSHPERPGFYTERMVL--KTEKLSAEISTSNEVTVTLLHGR 903
           +M  V+ ML SE M+L  P +PGF+ ER +L   T  ++ EI ++N  T++++  R
Sbjct: 732 NMSVVVLMLSSE-MLLLDPRQPGFFNERNLLFSDTVSINLEIPSNNFQTMSVIDPR 783

BLAST of MS025005 vs. TAIR 10
Match: AT4G21380.1 (receptor kinase 3 )

HSP 1 Score: 662.5 bits (1708), Expect = 4.7e-190
Identity = 357/842 (42.40%), Postives = 506/842 (60.10%), Query Frame = 0

Query: 136 FFFWTTIALFTRKSFASDSIHLKAGESIN-GSTRILVSAEQNFVLGIFTPQGSKFEYLGI 195
           FFF+  + LF   S ++++  L A ES+   S   +VS    F LG F P      YLGI
Sbjct: 13  FFFFFLLILFPAYSISANT--LSASESLTISSNNTIVSPGNVFELGFFKPGLDSRWYLGI 72

Query: 196 WYNYKNIPQTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS-SASAKTVKEPV 255
           WY   +  +T VWVANRD PL +S   LK + + N+V+L+++   +WS + +   V+ P+
Sbjct: 73  WYKAIS-KRTYVWVANRDTPLSSSIGTLKIS-DSNLVVLDQSDTPVWSTNLTGGDVRSPL 132

Query: 256 V-QLLDTGNLMLRE---FGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKLTSWESLN 315
           V +LLD GN +LR+      +  +WQSFD+P+DTLLP MKLGWD+KTG NR + SW+S +
Sbjct: 133 VAELLDNGNFVLRDSKNSAPDGVLWQSFDFPTDTLLPEMKLGWDAKTGFNRFIRSWKSPD 192

Query: 316 DPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFPKFEYEAG 375
           DPSSGDF++ +  +G P++ +    +  YR GPW G RFSG P ++   +    F     
Sbjct: 193 DPSSGDFSFKLETEGFPEIFLWNRESRMYRSGPWNGIRFSGVPEMQPFEYMVFNFTTSKE 252

Query: 376 EVTYSYD-AANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLCGDFGIC 435
           EVTYS+    +++  RL +++ G  ++F W +  + W   +  P D+CD+Y  CG +G C
Sbjct: 253 EVTYSFRITKSDVYSRLSISSSGLLQRFTWIETAQNWNQFWYAPKDQCDEYKECGVYGYC 312

Query: 436 TFSLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLPDSSGYL 495
             + +  C+C+ GF+P++PQ W     SDGCVR+    CG G+GF R+  +KLPD++   
Sbjct: 313 DSNTSPVCNCIKGFKPRNPQVWGLRDGSDGCVRKTLLSCGGGDGFVRLKKMKLPDTTTAS 372

Query: 496 VNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQDLYVRVA 555
           V+    +++CE  CL +C+C A+   ++R  G GCV W  +L D+R   + GQDLYVR+A
Sbjct: 373 VDRGIGVKECEQKCLRDCNCTAFANTDIRGSGSGCVTWTGELFDIRNYAKGGQDLYVRLA 432

Query: 556 ASEL-DSTNKKLMVAISVSVASFLGFLVLVICFILRRRRK-------------LRGKHFS 615
           A++L D  N+   +  S    S L  L  +I F+ +R++K             LR +   
Sbjct: 433 ATDLEDKRNRSAKIIGSSIGVSVLLLLSFIIFFLWKRKQKRSILIETPIVDHQLRSRDLL 492

Query: 616 SNTLI-------------------------------NLS---------------GKLPCG 675
            N ++                               N S               GKL  G
Sbjct: 493 MNEVVISSRRHISRENNTDDLELPLMEFEEVAMATNNFSNANKLGQGGFGIVYKGKLLDG 552

Query: 676 QEIAVKRLAEGSGQGQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLD 735
           QE+AVKRL++ S QG  EF+NEV LI++LQH NLV+LL  C+   E +L+YEY+ N SLD
Sbjct: 553 QEMAVKRLSKTSVQGTDEFKNEVKLIARLQHINLVRLLACCVDAGEKMLIYEYLENLSLD 612

Query: 736 HFLFDNERRSLLNWKKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKIS 795
             LFD  R S LNW+ R DII G+ARGLLYLH+DSR RIIHRDLK SNILLD  M PKIS
Sbjct: 613 SHLFDKSRNSKLNWQMRFDIINGIARGLLYLHQDSRFRIIHRDLKASNILLDKYMTPKIS 672

Query: 796 DFGMARMFGEDQTMTKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNR 855
           DFGMAR+FG D+T   T+++VGT+GYMSPEY +DG FS+KSD+FSFGV+LLEIIS  +N+
Sbjct: 673 DFGMARIFGRDETEANTRKVVGTYGYMSPEYAMDGIFSMKSDVFSFGVLLLEIISSKRNK 732

Query: 856 GFFHPDHQLNLLGHAWKLWDDGNALELMDAALED---RFQPSEALRCIQVGLLCVQQSPD 903
           GF++ D  LNLLG  W+ W +G  LE++D  + D    F+  E LRCIQ+GLLCVQ+  +
Sbjct: 733 GFYNSDRDLNLLGCVWRNWKEGKGLEIIDPIITDSSSTFRQHEILRCIQIGLLCVQERAE 792

BLAST of MS025005 vs. TAIR 10
Match: AT1G65790.1 (receptor kinase 1 )

HSP 1 Score: 649.8 bits (1675), Expect = 3.2e-186
Identity = 357/839 (42.55%), Postives = 499/839 (59.48%), Query Frame = 0

Query: 136 FFFWTTIALFTRKSFASDSIHLKAGESIN-GSTRILVSAEQNFVLGIFTPQGSKFEYLGI 195
           FF +  + LF   S + ++  L A ES+   S + ++S  Q F LG F P  S   YLGI
Sbjct: 11  FFIFLILILFLAFSVSPNT--LSATESLTISSNKTIISPSQIFELGFFNPASSSRWYLGI 70

Query: 196 WYNYKNIP-QTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS-SASAKTVKEP 255
           W  YK IP +T VWVANRDNPL +S+  LK +   N+V+ +++   +WS + +   V+ P
Sbjct: 71  W--YKIIPIRTYVWVANRDNPLSSSNGTLKIS-GNNLVIFDQSDRPVWSTNITGGDVRSP 130

Query: 256 V-VQLLDTGNLMLREFGSENYVWQSFDYPSDTLLPGMKLGWDSKTGLNRKLTSWESLNDP 315
           V  +LLD GN +LR+  +   +WQSFD+P+DTLL  MKLGWD KTG NR L SW++ +DP
Sbjct: 131 VAAELLDNGNFLLRD-SNNRLLWQSFDFPTDTLLAEMKLGWDQKTGFNRILRSWKTTDDP 190

Query: 316 SSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFPKFEYEAGEV 375
           SSG+F+  +     P+  I    +  YR GPW G RFS  P   +  +    F     EV
Sbjct: 191 SSGEFSTKLETSEFPEFYICSKESILYRSGPWNGMRFSSVPGTIQVDYMVYNFTASKEEV 250

Query: 376 TYSYDA-ANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLCGDFGICTF 435
           TYSY     NL  RL LN+ G  ++  W +  + W  L+  P D CD+Y +CG+FG C  
Sbjct: 251 TYSYRINKTNLYSRLYLNSAGLLQRLTWFETTQSWKQLWYSPKDLCDNYKVCGNFGYCDS 310

Query: 436 SLTAQCDCMAGFEPKSPQDWEKLRWSDGCVRRDSQICGNGEGFKRISSVKLPDSSGYLVN 495
           +    C C+ GF+P + Q W+    S GC+R+    C   +GF R+  +KLPD++  +V+
Sbjct: 311 NSLPNCYCIKGFKPVNEQAWDLRDGSAGCMRKTRLSCDGRDGFTRLKRMKLPDTTATIVD 370

Query: 496 VNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQDLYVRVAAS 555
               ++ C+  CL +C+C A+   ++R GG GCV W ++++D+R   + GQDLYVR+AA+
Sbjct: 371 REIGLKVCKERCLEDCNCTAFANADIRNGGSGCVIWTREILDMRNYAKGGQDLYVRLAAA 430

Query: 556 ELDS---TNKKLM-VAISVSVASFLGFLVL--------------------------VICF 615
           EL+     N+K++  +I VS+   L F++                           +I  
Sbjct: 431 ELEDKRIKNEKIIGSSIGVSILLLLSFVIFHFWKRKQKRSITIQTPNVDQVRSQDSLIND 490

Query: 616 ILRRRRKLRGKHFSSNTL-----------------------------INLSGKLPCGQEI 675
           ++  RR    K   S  L                             I   G+L  G+EI
Sbjct: 491 VVVSRRGYTSKEKKSEYLELPLLELEALATATNNFSNDNKLGQGGFGIVYKGRLLDGKEI 550

Query: 676 AVKRLAEGSGQGQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKSLDHFL 735
           AVKRL++ S QG  EF NEV LI++LQH NLV+LLG C+ K E +L+YEY+ N SLD  L
Sbjct: 551 AVKRLSKMSSQGTDEFMNEVRLIAKLQHINLVRLLGCCVDKGEKMLIYEYLENLSLDSHL 610

Query: 736 FDNERRSLLNWKKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPKISDFG 795
           FD  R S LNW+KR DII G+ARGLLYLH+DSR RIIHRDLK SN+LLD  M PKISDFG
Sbjct: 611 FDQTRSSNLNWQKRFDIINGIARGLLYLHQDSRCRIIHRDLKASNVLLDKNMTPKISDFG 670

Query: 796 MARMFGEDQTMTKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMKNRGFF 855
           MAR+FG ++T   T+R+VGT+GYMSPEY +DG FS+KSD+FSFGV+LLEIISG +N+GF+
Sbjct: 671 MARIFGREETEANTRRVVGTYGYMSPEYAMDGIFSMKSDVFSFGVLLLEIISGKRNKGFY 730

Query: 856 HPDHQLNLLGHAWKLWDDGNALELMDA----ALEDRFQPSEALRCIQVGLLCVQQSPDER 903
           + +  LNLLG  W+ W +GN LE++D     +L  +F   E LRCIQ+GLLCVQ+  ++R
Sbjct: 731 NSNRDLNLLGFVWRHWKEGNELEIVDPINIDSLSSKFPTHEILRCIQIGLLCVQERAEDR 790

BLAST of MS025005 vs. TAIR 10
Match: AT4G27300.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 642.9 bits (1657), Expect = 3.9e-184
Identity = 346/783 (44.19%), Postives = 480/783 (61.30%), Query Frame = 0

Query: 170 LVSAEQNFVLGIFT---PQGSKFEYLGIWYNYKNIPQTVVWVANRDNPLVNSSARLKFNE 229
           L S +Q F LG F+    +  +  +LG+WY     P  VVWVANR+NPL  +S  L  + 
Sbjct: 40  LSSPDQVFQLGFFSLDQEEQPQHRFLGLWYME---PFAVVWVANRNNPLYGTSGFLNLSS 99

Query: 230 EGNIVLLNETGGVLWSSAS-----AKTVKEPVVQLLDTGNLMLREFGSENYVWQSFDYPS 289
            G++ L +     LWSS+S     +KT   P++++  +GNL+  + G E  +WQSFDYP 
Sbjct: 100 LGDLQLFDGEHKALWSSSSSSTKASKTANNPLLKISCSGNLISSD-GEEAVLWQSFDYPM 159

Query: 290 DTLLPGMKLGWDSKTGLNRKLTSWESLNDPSSGDFNYSVALDGLPQLVIRKGPNT--TYR 349
           +T+L GMKLG + KT +   L+SW++L DPS GDF  S+   GLPQL++RK  ++  +YR
Sbjct: 160 NTILAGMKLGKNFKTQMEWSLSSWKTLKDPSPGDFTLSLDTRGLPQLILRKNGDSSYSYR 219

Query: 350 GGPWYGTRFSGSPPL-RENPFYFPKFEYEAGEVTYSYDAANNLTLRLVLNADGTFEQFYW 409
            G W G  F+G+P + REN  +  KF   A EV YS+   + +  RLVLN  G   +F  
Sbjct: 220 LGSWNGLSFTGAPAMGRENSLFDYKFTSSAQEVNYSWTPRHRIVSRLVLNNTGKLHRFIQ 279

Query: 410 DDGGKYWYPLYILPGDRCDDYGLCGDFGICTFSL--TAQCDCMAGFEPKSPQDWEKLRWS 469
               + W      P D CD Y +CG + +C  +   T  C C+ GF+PKS + W   R +
Sbjct: 280 SKQNQ-WILANTAPEDECDYYSICGAYAVCGINSKNTPSCSCLQGFKPKSGRKWNISRGA 339

Query: 470 DGCVRRDSQICGNGEGFKRISSVKLPDS--SGYLVNVNTSIQDCEAACLNNCSCLAYGIM 529
            GCV      C   + F +   +KLPD+  S Y      +++DC+  C +NCSC AY   
Sbjct: 340 YGCVHEIPTNCEKKDAFVKFPGLKLPDTSWSWYDAKNEMTLEDCKIKCSSNCSCTAYANT 399

Query: 530 EVRTGGYGCVNWFQKLMDVRFVPENGQDLYVRVAASELDSTNKKLMVAISVSVASFLGFL 589
           ++R GG GC+ WF  L+D+R     GQD+Y+R+  ++++   ++++  +  SV +    L
Sbjct: 400 DIREGGKGCLLWFGDLVDMREYSSFGQDVYIRMGFAKIEFKGREVVGMVVGSVVAIAVVL 459

Query: 590 VLVI-CFILRRRRKLRGKHFSSN-----------------------TLINL--------- 649
           V+V  CF  +  ++ RG++F                          + +N          
Sbjct: 460 VVVFACFRKKIMKRYRGENFRKGIEEEDLDLPIFDRKTISIATDDFSYVNFLGRGGFGPV 519

Query: 650 -SGKLPCGQEIAVKRLAEGSGQGQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYE 709
             GKL  GQEIAVKRL+  SGQG  EF+NEV LI++LQHRNLV+LLG CI  EE +L+YE
Sbjct: 520 YKGKLEDGQEIAVKRLSANSGQGVEEFKNEVKLIAKLQHRNLVRLLGCCIQGEECMLIYE 579

Query: 710 YMPNKSLDHFLFDNERRSLLNWKKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLD 769
           YMPNKSLD F+FD  R + L+WKKR++II GVARG+LYLH+DSRLRIIHRDLK  N+LLD
Sbjct: 580 YMPNKSLDFFIFDERRSTELDWKKRMNIINGVARGILYLHQDSRLRIIHRDLKAGNVLLD 639

Query: 770 NEMNPKISDFGMARMFGEDQTMTKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLE 829
           N+MNPKISDFG+A+ FG DQ+ + T R+VGT+GYM PEY +DG FS+KSD+FSFGV++LE
Sbjct: 640 NDMNPKISDFGLAKSFGGDQSESSTNRVVGTYGYMPPEYAIDGHFSVKSDVFSFGVLVLE 699

Query: 830 IISGMKNRGFFHPDHQLNLLGHAWKLWDDGNALELMDAA-LEDRFQPSEALRCIQVGLLC 889
           II+G  NRGF H DH LNLLGH WK+W +   +E+ +   LE+     E LRCI V LLC
Sbjct: 700 IITGKTNRGFRHADHDLNLLGHVWKMWVEDREIEVPEEEWLEETSVIPEVLRCIHVALLC 759

Query: 890 VQQSPDERPSMWSVLSMLESENMVLSHPERPGFYTERMVLKTEKLSAEISTSNEVTVTLL 903
           VQQ P++RP+M SV+ M  S++  L HP +PGF+T R V      S  + + NEV++T+L
Sbjct: 760 VQQKPEDRPTMASVVLMFGSDSS-LPHPTQPGFFTNRNVPDISS-SLSLRSQNEVSITML 815

BLAST of MS025005 vs. TAIR 10
Match: AT1G65800.1 (receptor kinase 2 )

HSP 1 Score: 632.1 bits (1629), Expect = 6.8e-181
Identity = 355/844 (42.06%), Postives = 492/844 (58.29%), Query Frame = 0

Query: 136 FFFWTTIALFTRKSFASDSIHLKAGESIN-GSTRILVSAEQNFVLGIFTPQGSKFEYLGI 195
           +F    I LF   +F+  + +  A ES+   S + ++S  Q F LG F P  S   YLGI
Sbjct: 11  YFILFIIILFL--AFSVYASNFSATESLTISSNKTIISPSQIFELGFFNPDSSSRWYLGI 70

Query: 196 WYNYKNIP-QTVVWVANRDNPLVNSSARLKFNEEGNIVLLNETGGVLWS-SASAKTVKEP 255
           W  YK IP +T VWVANRDNPL +S+  LK + + N+V+ +++   +WS + +   V+ P
Sbjct: 71  W--YKIIPIRTYVWVANRDNPLSSSNGTLKIS-DNNLVIFDQSDRPVWSTNITGGDVRSP 130

Query: 256 V-VQLLDTGNLMLREFGS---ENYVWQSFDYPSDTLLPGMKLGWDSKT-GLNRKLTSWES 315
           V  +LLD GN +LR+  +     ++WQSFD+P+DTLL  MK+GWD+K+ G NR L SW++
Sbjct: 131 VAAELLDYGNFVLRDSKNNKPSGFLWQSFDFPTDTLLSDMKMGWDNKSGGFNRILRSWKT 190

Query: 316 LNDPSSGDFNYSVALDGLPQLVIRKGPNTTYRGGPWYGTRFSGSPPLRENPFYFPKFEYE 375
            +DPSSGDF+  +   G P+  I    + TYR GPW G RFS  P ++   +    F   
Sbjct: 191 TDDPSSGDFSTKLRTSGFPEFYIYNKESITYRSGPWLGNRFSSVPGMKPVDYIDNSFTEN 250

Query: 376 AGEVTYSYDA-ANNLTLRLVLNADGTFEQFYWDDGGKYWYPLYILPGDRCDDYGLCGDFG 435
             +V YSY     N+   L L++ G  ++  W +  + W  L+  P D CD+Y  CG++G
Sbjct: 251 NQQVVYSYRVNKTNIYSILSLSSTGLLQRLTWMEAAQSWKQLWYSPKDLCDNYKECGNYG 310

Query: 436 ICTFSLTAQCDCMAGFEPKSPQDWEKLR-WSDGCVRRDSQICGNGEGFKRISSVKLPDSS 495
            C  + +  C+C+ GFEP + Q    LR  S GCVR+    C   +GF R+  ++LPD++
Sbjct: 311 YCDANTSPICNCIKGFEPMNEQ--AALRDDSVGCVRKTKLSCDGRDGFVRLKKMRLPDTT 370

Query: 496 GYLVNVNTSIQDCEAACLNNCSCLAYGIMEVRTGGYGCVNWFQKLMDVRFVPENGQDLYV 555
              V+    +++CE  CL  C+C A+   ++R GG GCV W   L D+R   + GQDLYV
Sbjct: 371 ETSVDKGIGLKECEERCLKGCNCTAFANTDIRNGGSGCVIWSGGLFDIRNYAKGGQDLYV 430

Query: 556 RVAASELDSTNKKLMVAISVSV-ASFLGFLVLVICFILRRRRK------------LRGKH 615
           RVAA +L+    K    I  S+  S L  L  +I    +R++K            +R + 
Sbjct: 431 RVAAGDLEDKRIKSKKIIGSSIGVSILLLLSFIIFHFWKRKQKRSITIQTPIVDLVRSQD 490

Query: 616 FSSNTLINLS----------------------------------------------GKLP 675
              N L+  S                                              G L 
Sbjct: 491 SLMNELVKASRSYTSKENKTDYLELPLMEWKALAMATNNFSTDNKLGQGGFGIVYKGMLL 550

Query: 676 CGQEIAVKRLAEGSGQGQSEFRNEVLLISQLQHRNLVKLLGFCIHKEETLLVYEYMPNKS 735
            G+EIAVKRL++ S QG  EF NEV LI++LQH NLV+LLG C+ K E +L+YEY+ N S
Sbjct: 551 DGKEIAVKRLSKMSSQGTDEFMNEVRLIAKLQHINLVRLLGCCVDKGEKMLIYEYLENLS 610

Query: 736 LDHFLFDNERRSLLNWKKRVDIIIGVARGLLYLHRDSRLRIIHRDLKVSNILLDNEMNPK 795
           LD  LFD  R S LNW+KR DII G+ARGLLYLH+DSR RIIHRDLK SN+LLD  M PK
Sbjct: 611 LDSHLFDQTRSSNLNWQKRFDIINGIARGLLYLHQDSRCRIIHRDLKASNVLLDKNMTPK 670

Query: 796 ISDFGMARMFGEDQTMTKTQRIVGTFGYMSPEYVLDGCFSLKSDIFSFGVILLEIISGMK 855
           ISDFGMAR+FG ++T   T+R+VGT+GYMSPEY +DG FS+KSD+FSFGV+LLEIISG +
Sbjct: 671 ISDFGMARIFGREETEANTRRVVGTYGYMSPEYAMDGIFSMKSDVFSFGVLLLEIISGKR 730

Query: 856 NRGFFHPDHQLNLLGHAWKLWDDGNALELMDA----ALEDRFQPSEALRCIQVGLLCVQQ 903
           N+GF++ +  LNLLG  W+ W +G  LE++D     AL   F   E LRCIQ+GLLCVQ+
Sbjct: 731 NKGFYNSNRDLNLLGFVWRHWKEGKELEIVDPINIDALSSEFPTHEILRCIQIGLLCVQE 790