MS025001 (gene) Bitter gourd (TR) v1

Overview
NameMS025001
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionReceptor-like serine/threonine-protein kinase
Locationscaffold96: 268867 .. 270993 (-)
RNA-Seq ExpressionMS025001
SyntenyMS025001
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGACTTCAAACGGATTGGAGATTTCCACTTCGTTTTCTTCTTCTACTTCTTTCATTTTGCTACGGCAGATCAGATACCATCACATCGACAAATTTCATCAAAGACCCTGCAACTATAGCTTCCAATGGCAGTACCTTCAAGTTGGGATTTTTCTCACCTTTAAATTCTACCCGCCGATATGTCGGAATTTGGCTCAACAACATTTCCGTACAAACTATAGTATGGGTAGCCAACAGAGACAGCCCTCTGAACGACACTTCTGGGATCTTCACAATCTCAAAGGATGGGAATCTTGTGGTGTTGGATGGAAACGACACCGTCCTCTGGTCTTCAAATGTTTCTTCTCCTTTAGTAAACACAAGCGCCCAAATTTTAGATACCGGTAACCTCATTTTGCAAGATGCTGTTTCCGGGTTTGTTATATGGGAGAGTTTCCAACACCCTTCTAATGTATTCCTGTATTCCATGAAACTTATCACAAACAAAGACATGAATTCCAAAGACAAGGTTCAGCTTACCTCTTGGAAAAACCCTTCAGATCCATCTACAGGAAACTTTTTGTTGGGGCTGGATGTTCAATTTCTTCCAGAATTGGTGATTTGGAATGGCCGTAATCCTTATTGGCGGTCTGGTCCATGGAACGGTAATGCTTTTATCGGAAGACCCGAAATGATTGATGTTTATCTCTCGGGATTTAATCTTGTAATCGGAGACAACACCTACACTCTCTCTACTCCTTATCGTAGTGTTGATATGCAAGAATATGAATTTTTATCTTTGAGCTCACAAGGGAATTGGGAGCAGAAGTACTGGGATGCTAAGGAGGCGCAGTTGAAGAATTATTGGTCGGCTCTAGGAACGCAGTGTGATTTTTATGGTGCTTGTGGGACGGCTGGGATTTGTGATGTGAAAGCGTCCCCTGTTTGCAGCTGTATAAAAGGGTTTAAGCCAAAGCATGAAGAGGAATGGAATAAAGGAAATTGGAGCAGGGGATGTGTGAGAAAGACGCCATTGAATTGTACTCACAACTCCAACAACGCCACCGCTGAGGCTGCAGATGGGTTTTTTAAATTGGAAGCGATTAAACTGCCATTTTTTGAACAGTGGTACGAGGCCTCGATCTATGAAGACGATTGCAGACAGGAGTGCTTGAGGAATTGTTCTTGTACTGCTTATGCATTTGAAAATGGTATTGGTTGTATGCTATGGAGAAGGGATGATTTAATTGATATACAAAAGTTTGAGAGTGGTGGAGCTGATCTTTATCTTCGAATGGCGTATGCAGACTTGGATCATACTACAAGTATATATTCTCCATATAAATTTATACTGCAATTTTTTTCATCATTGAAATTATTTGTATATATATTGTCTTCTAACATTATTTTCATCAACACACAGCAGTTGATCAGGTAAAAGACAAGAAACGAATTATTATAGCCATAGTGCTACCAGGAACGTTTATCATCTTCATTACCATTGCCATATACTTTTGGTGGAGATTGAAGACTCACAAACATGGTAATTAAACGTACAGTACTTGATCATTTCATTTCCTTACCATTTTTACTAGTGGATAAAATAGTCATATGAAAGTAAAATTTGAGGTTTATAAAACAGAGAAGAAGACAAGTATCAAATCCAGCAAAAAGGGAAAGATTTTGAAATGCACAAGGGATGATGATACGATTGGGGATGAAATTGAACTCGAAGAGTTGCCTCTTTATGATTTTGAAGAGCTGGCAATTGCAACAAACAACTTTGATTTAAGTAACCAGCTTGGGCAGGGTGGCTTTGGTCCAGTATATAAGGTAAAGTAATACTTCTGTAGATCCTTTTTTTTCTCTCTCTTTCTTTTGATTAACTGGGAGCACGGATAGTTGAAGAAATCATGTTTCTTTTTTTCAGGGAAAATTATTAAATGGACAGGAAATAGCAATAAAGAGACTTTCAAGAACATCTAACCAAGGGTACGAAGAATTTATAACTGAAGTGAAAGTGATTTCAAAATTACAACATAGGAATCTTGTGCAGCTTCTCGGTTGTTGCATTGAAGGAGAAGAGAAGATGTTAATATATGAGTATATGGCCAATGGAAGTTTGGATGCGATGATATTTGGTTAG

mRNA sequence

ATGAGACTTCAAACGGATTGGAGATTTCCACTTCGTTTTCTTCTTCTACTTCTTTCATTTTGCTACGGCAGATCAGATACCATCACATCGACAAATTTCATCAAAGACCCTGCAACTATAGCTTCCAATGGCAGTACCTTCAAGTTGGGATTTTTCTCACCTTTAAATTCTACCCGCCGATATGTCGGAATTTGGCTCAACAACATTTCCGTACAAACTATAGTATGGGTAGCCAACAGAGACAGCCCTCTGAACGACACTTCTGGGATCTTCACAATCTCAAAGGATGGGAATCTTGTGGTGTTGGATGGAAACGACACCGTCCTCTGGTCTTCAAATGTTTCTTCTCCTTTAGTAAACACAAGCGCCCAAATTTTAGATACCGGTAACCTCATTTTGCAAGATGCTGTTTCCGGGTTTGTTATATGGGAGAGTTTCCAACACCCTTCTAATGTATTCCTGTATTCCATGAAACTTATCACAAACAAAGACATGAATTCCAAAGACAAGGTTCAGCTTACCTCTTGGAAAAACCCTTCAGATCCATCTACAGGAAACTTTTTGTTGGGGCTGGATGTTCAATTTCTTCCAGAATTGGTGATTTGGAATGGCCGTAATCCTTATTGGCGGTCTGGTCCATGGAACGGTAATGCTTTTATCGGAAGACCCGAAATGATTGATGTTTATCTCTCGGGATTTAATCTTGTAATCGGAGACAACACCTACACTCTCTCTACTCCTTATCGTAGTGTTGATATGCAAGAATATGAATTTTTATCTTTGAGCTCACAAGGGAATTGGGAGCAGAAGTACTGGGATGCTAAGGAGGCGCAGTTGAAGAATTATTGGTCGGCTCTAGGAACGCAGTGTGATTTTTATGGTGCTTGTGGGACGGCTGGGATTTGTGATGTGAAAGCGTCCCCTGTTTGCAGCTGTATAAAAGGGTTTAAGCCAAAGCATGAAGAGGAATGGAATAAAGGAAATTGGAGCAGGGGATGTGTGAGAAAGACGCCATTGAATTGTACTCACAACTCCAACAACGCCACCGCTGAGGCTGCAGATGGGTTTTTTAAATTGGAAGCGATTAAACTGCCATTTTTTGAACAGTGGTACGAGGCCTCGATCTATGAAGACGATTGCAGACAGGAGTGCTTGAGGAATTGTTCTTGTACTGCTTATGCATTTGAAAATGGTATTGGTTGTATGCTATGGAGAAGGGATGATTTAATTGATATACAAAAGTTTGAGAGTGGTGGAGCTGATCTTTATCTTCGAATGGCGTATGCAGACTTGGATCATACTACAACAGTTGATCAGGTAAAAGACAAGAAACGAATTATTATAGCCATAGTGCTACCAGGAACGTTTATCATCTTCATTACCATTGCCATATACTTTTGGTGGAGATTGAAGACTCACAAACATGAGAAGAAGACAAGTATCAAATCCAGCAAAAAGGGAAAGATTTTGAAATGCACAAGGGATGATGATACGATTGGGGATGAAATTGAACTCGAAGAGTTGCCTCTTTATGATTTTGAAGAGCTGGCAATTGCAACAAACAACTTTGATTTAAGTAACCAGCTTGGGCAGGGTGGCTTTGGTCCAGTATATAAGGGAAAATTATTAAATGGACAGGAAATAGCAATAAAGAGACTTTCAAGAACATCTAACCAAGGGTACGAAGAATTTATAACTGAAGTGAAAGTGATTTCAAAATTACAACATAGGAATCTTGTGCAGCTTCTCGGTTGTTGCATTGAAGGAGAAGAGAAGATGTTAATATATGAGTATATGGCCAATGGAAGTTTGGATGCGATGATATTTGGTTAG

Coding sequence (CDS)

ATGAGACTTCAAACGGATTGGAGATTTCCACTTCGTTTTCTTCTTCTACTTCTTTCATTTTGCTACGGCAGATCAGATACCATCACATCGACAAATTTCATCAAAGACCCTGCAACTATAGCTTCCAATGGCAGTACCTTCAAGTTGGGATTTTTCTCACCTTTAAATTCTACCCGCCGATATGTCGGAATTTGGCTCAACAACATTTCCGTACAAACTATAGTATGGGTAGCCAACAGAGACAGCCCTCTGAACGACACTTCTGGGATCTTCACAATCTCAAAGGATGGGAATCTTGTGGTGTTGGATGGAAACGACACCGTCCTCTGGTCTTCAAATGTTTCTTCTCCTTTAGTAAACACAAGCGCCCAAATTTTAGATACCGGTAACCTCATTTTGCAAGATGCTGTTTCCGGGTTTGTTATATGGGAGAGTTTCCAACACCCTTCTAATGTATTCCTGTATTCCATGAAACTTATCACAAACAAAGACATGAATTCCAAAGACAAGGTTCAGCTTACCTCTTGGAAAAACCCTTCAGATCCATCTACAGGAAACTTTTTGTTGGGGCTGGATGTTCAATTTCTTCCAGAATTGGTGATTTGGAATGGCCGTAATCCTTATTGGCGGTCTGGTCCATGGAACGGTAATGCTTTTATCGGAAGACCCGAAATGATTGATGTTTATCTCTCGGGATTTAATCTTGTAATCGGAGACAACACCTACACTCTCTCTACTCCTTATCGTAGTGTTGATATGCAAGAATATGAATTTTTATCTTTGAGCTCACAAGGGAATTGGGAGCAGAAGTACTGGGATGCTAAGGAGGCGCAGTTGAAGAATTATTGGTCGGCTCTAGGAACGCAGTGTGATTTTTATGGTGCTTGTGGGACGGCTGGGATTTGTGATGTGAAAGCGTCCCCTGTTTGCAGCTGTATAAAAGGGTTTAAGCCAAAGCATGAAGAGGAATGGAATAAAGGAAATTGGAGCAGGGGATGTGTGAGAAAGACGCCATTGAATTGTACTCACAACTCCAACAACGCCACCGCTGAGGCTGCAGATGGGTTTTTTAAATTGGAAGCGATTAAACTGCCATTTTTTGAACAGTGGTACGAGGCCTCGATCTATGAAGACGATTGCAGACAGGAGTGCTTGAGGAATTGTTCTTGTACTGCTTATGCATTTGAAAATGGTATTGGTTGTATGCTATGGAGAAGGGATGATTTAATTGATATACAAAAGTTTGAGAGTGGTGGAGCTGATCTTTATCTTCGAATGGCGTATGCAGACTTGGATCATACTACAACAGTTGATCAGGTAAAAGACAAGAAACGAATTATTATAGCCATAGTGCTACCAGGAACGTTTATCATCTTCATTACCATTGCCATATACTTTTGGTGGAGATTGAAGACTCACAAACATGAGAAGAAGACAAGTATCAAATCCAGCAAAAAGGGAAAGATTTTGAAATGCACAAGGGATGATGATACGATTGGGGATGAAATTGAACTCGAAGAGTTGCCTCTTTATGATTTTGAAGAGCTGGCAATTGCAACAAACAACTTTGATTTAAGTAACCAGCTTGGGCAGGGTGGCTTTGGTCCAGTATATAAGGGAAAATTATTAAATGGACAGGAAATAGCAATAAAGAGACTTTCAAGAACATCTAACCAAGGGTACGAAGAATTTATAACTGAAGTGAAAGTGATTTCAAAATTACAACATAGGAATCTTGTGCAGCTTCTCGGTTGTTGCATTGAAGGAGAAGAGAAGATGTTAATATATGAGTATATGGCCAATGGAAGTTTGGATGCGATGATATTTGGTTAG

Protein sequence

MRLQTDWRFPLRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRRYVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVNTSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPSDPSTGNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLVIGDNTYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAGICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLEAIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGGADLYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTSIKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKGKLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMANGSLDAMIFG
Homology
BLAST of MS025001 vs. NCBI nr
Match: XP_022139811.1 (G-type lectin S-receptor-like serine/threonine-protein kinase SD1-13 isoform X2 [Momordica charantia])

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 590/609 (96.88%), Postives = 595/609 (97.70%), Query Frame = 0

Query: 1   MRLQTDWRFPLRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRR 60
           MRLQTDWRF LRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRR
Sbjct: 1   MRLQTDWRFSLRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRR 60

Query: 61  YVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVN 120
           YVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVN
Sbjct: 61  YVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVN 120

Query: 121 TSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPS 180
           TSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPS
Sbjct: 121 TSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPS 180

Query: 181 DPSTGNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLVIGDN 240
           DPSTGNFL GLDVQ+LPELVIWNGRNPYWRSGPWNGN FIGRPEM D+YLSG+NLVIGDN
Sbjct: 181 DPSTGNFLFGLDVQYLPELVIWNGRNPYWRSGPWNGNTFIGRPEMTDIYLSGYNLVIGDN 240

Query: 241 TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG 300
           TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG
Sbjct: 241 TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG 300

Query: 301 ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE 360
           ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE
Sbjct: 301 ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE 360

Query: 361 AIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGGA 420
            IKLPF EQW  AS YED+CRQEC  NCSCTAYAFE GIGCMLWRRDDLIDIQKFESGGA
Sbjct: 361 TIKLPFLEQWSNASYYEDECRQECFTNCSCTAYAFEKGIGCMLWRRDDLIDIQKFESGGA 420

Query: 421 DLYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTS 480
           DLYLRMAYADLDHTT  +QVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTS
Sbjct: 421 DLYLRMAYADLDHTT--NQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTS 480

Query: 481 IKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKG 540
           IKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKG
Sbjct: 481 IKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKG 540

Query: 541 KLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMA 600
           KLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMA
Sbjct: 541 KLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMA 600

Query: 601 NGSLDAMIF 610
           NGSLDAMIF
Sbjct: 601 NGSLDAMIF 607

BLAST of MS025001 vs. NCBI nr
Match: XP_022139810.1 (G-type lectin S-receptor-like serine/threonine-protein kinase SD1-13 isoform X1 [Momordica charantia])

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 590/609 (96.88%), Postives = 595/609 (97.70%), Query Frame = 0

Query: 1   MRLQTDWRFPLRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRR 60
           MRLQTDWRF LRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRR
Sbjct: 1   MRLQTDWRFSLRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRR 60

Query: 61  YVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVN 120
           YVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVN
Sbjct: 61  YVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVN 120

Query: 121 TSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPS 180
           TSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPS
Sbjct: 121 TSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPS 180

Query: 181 DPSTGNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLVIGDN 240
           DPSTGNFL GLDVQ+LPELVIWNGRNPYWRSGPWNGN FIGRPEM D+YLSG+NLVIGDN
Sbjct: 181 DPSTGNFLFGLDVQYLPELVIWNGRNPYWRSGPWNGNTFIGRPEMTDIYLSGYNLVIGDN 240

Query: 241 TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG 300
           TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG
Sbjct: 241 TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG 300

Query: 301 ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE 360
           ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE
Sbjct: 301 ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE 360

Query: 361 AIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGGA 420
            IKLPF EQW  AS YED+CRQEC  NCSCTAYAFE GIGCMLWRRDDLIDIQKFESGGA
Sbjct: 361 TIKLPFLEQWSNASYYEDECRQECFTNCSCTAYAFEKGIGCMLWRRDDLIDIQKFESGGA 420

Query: 421 DLYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTS 480
           DLYLRMAYADLDHTT  +QVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTS
Sbjct: 421 DLYLRMAYADLDHTT--NQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTS 480

Query: 481 IKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKG 540
           IKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKG
Sbjct: 481 IKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKG 540

Query: 541 KLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMA 600
           KLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMA
Sbjct: 541 KLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMA 600

Query: 601 NGSLDAMIF 610
           NGSLDAMIF
Sbjct: 601 NGSLDAMIF 607

BLAST of MS025001 vs. NCBI nr
Match: XP_022140159.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111010891 [Momordica charantia])

HSP 1 Score: 861.7 bits (2225), Expect = 3.9e-246
Identity = 433/613 (70.64%), Postives = 503/613 (82.06%), Query Frame = 0

Query: 10  PLRFLLLLLSF-CYGRS-----DTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRRYVG 69
           PL  LLLLLSF C+  +     DTITSTNFIKDPATI+SN S+F+LGFF+P NSTRRYVG
Sbjct: 12  PLLHLLLLLSFTCFSSTFCSAKDTITSTNFIKDPATISSNSSSFELGFFAPPNSTRRYVG 71

Query: 70  IWLNNISVQTIVWVANRDSPLNDTS-GIFTISKDGNLVVLDGNDTVLWSSNV-SSPLVNT 129
           IW N +SVQT++WVANRD+PLN+TS GIFTISKDGNLVVLDGNDTVLWSS+V SS   N 
Sbjct: 72  IWFNQVSVQTLIWVANRDNPLNNTSQGIFTISKDGNLVVLDGNDTVLWSSDVSSSSSTNR 131

Query: 130 SAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNK-DMNSKDKVQLTSWKNPS 189
           SA+ILD+GNL+L+DA SG VIW+SF+HPS+ FL +M++ITNK D+NSKDKVQLTSWKNPS
Sbjct: 132 SARILDSGNLVLEDASSGEVIWQSFKHPSDKFLPAMQIITNKLDVNSKDKVQLTSWKNPS 191

Query: 190 DPSTGNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLVIGDN 249
           DPSTGNF  G+DVQ LPE+V+WNGR+PYWRSGPWNGN+FIG PEM  VYLSG++LVI D 
Sbjct: 192 DPSTGNFSFGMDVQNLPEVVVWNGRDPYWRSGPWNGNSFIGVPEMEAVYLSGYSLVIQDQ 251

Query: 250 TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG 309
           TYTLS  Y +  +QE+ +L LS  GN +Q YWD  E + K  W +L T+CD YGACG  G
Sbjct: 252 TYTLSVSY-NYSIQEFAYLFLSPGGNLQQTYWDVSEERWKITWMSLQTRCDLYGACGAFG 311

Query: 310 ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE 369
           IC+ K SPVCSC+KGFKP HEEEWN+GNWS GCVR TPL C     NAT    DGF K+E
Sbjct: 312 ICNPKPSPVCSCLKGFKPNHEEEWNQGNWSGGCVRNTPLLC-----NATT-LEDGFLKVE 371

Query: 370 AIKLPFFEQWYE-ASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGG 429
            +KLPF  +W   +S+  DDCRQ CL+NCSCTAYA+ENGI CMLWRRDDLID+QKFESGG
Sbjct: 372 TVKLPFLAEWSGISSLTADDCRQLCLKNCSCTAYAYENGINCMLWRRDDLIDMQKFESGG 431

Query: 430 ADLYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFW--WRLKTHKHEK 489
           ADLY+RMAYADLDHTT  D+VKDK  IIIAIVLP T +IF+ IAIY W  W+ K  K+EK
Sbjct: 432 ADLYVRMAYADLDHTTN-DRVKDKTGIIIAIVLPTTLVIFV-IAIYLWLRWKRKAPKNEK 491

Query: 490 KTSIKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPV 549
           K ++ S +K KILK  R+DD I D+I+LEELP+YD E+LA+ATNNFDL+N+LGQGGFGPV
Sbjct: 492 KKTMASGEKEKILKLKREDDMIEDDIKLEELPIYDLEKLAMATNNFDLTNKLGQGGFGPV 551

Query: 550 YKGKLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYE 609
           YKGKL NGQEIA+KRLSR S QGYEEFI EV+VISKLQHRNLV+L GCCIEGEEKMLIYE
Sbjct: 552 YKGKLANGQEIAVKRLSRVSQQGYEEFINEVRVISKLQHRNLVRLFGCCIEGEEKMLIYE 611

Query: 610 YMANGSLDAMIFG 611
           YM N SLDA+IFG
Sbjct: 612 YMPNLSLDALIFG 615

BLAST of MS025001 vs. NCBI nr
Match: XP_022140157.1 (uncharacterized protein LOC111010889 [Momordica charantia])

HSP 1 Score: 807.7 bits (2085), Expect = 6.6e-230
Identity = 414/624 (66.35%), Postives = 479/624 (76.76%), Query Frame = 0

Query: 1   MRLQTDWRFPLRFLLLL--------LSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFF 60
           M+ QT+W F  R LL+L        L  C GR DTITSTNFIKDP TI SN S+F LGFF
Sbjct: 1   MKPQTNWSFSCRLLLILSFTCFSSRLLICSGR-DTITSTNFIKDPETITSNASSFHLGFF 60

Query: 61  SPLNSTRRYVGIW-LNNISVQTIVWVANRDSPLNDTS-GIFTISKDGNLVVLDGNDTVLW 120
           +P NSTRRYVGIW +N I  QT+VWVANRD+PLN+TS GIFTISKDGNLVVLDGN+TVLW
Sbjct: 61  TPPNSTRRYVGIWFINQIPQQTVVWVANRDNPLNNTSQGIFTISKDGNLVVLDGNNTVLW 120

Query: 121 SSNVSSPLVNTSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDK 180
           SSNVSSP  N SA+ILD+GNL+L+DA SG VIWESF+HPS+ FL SMKLITN    +++K
Sbjct: 121 SSNVSSPATNRSARILDSGNLVLEDASSGEVIWESFKHPSDKFLTSMKLITN--AKTREK 180

Query: 181 VQLTSWKNPSDPSTGNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYL 240
           V+LTSW  PSDPSTGNF LG+ V  +PE VIW GRN YWRSGPWNG  FIG PEM   YL
Sbjct: 181 VELTSWNTPSDPSTGNFSLGIYVHNIPEAVIWKGRNTYWRSGPWNGQNFIGIPEMDSTYL 240

Query: 241 SGFNLVIGDNTYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDA-KEAQLKNYWSALGTQ 300
           SG+ L I D +Y  S  Y   D +++ +L LSSQGN  +   D   E + +  WSAL TQ
Sbjct: 241 SGYKLTIEDQSYHFSVAYN--DDEQFGYLFLSSQGNLVETNVDLDDERRWRATWSALQTQ 300

Query: 301 CDFYGACGTAGICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNAT 360
           CD YG CG  GICD KASP+CSC++GFKP HE++WN+GNWS GCVRKTPL C     N+T
Sbjct: 301 CDIYGTCGAFGICDAKASPICSCLRGFKPNHEQDWNRGNWSGGCVRKTPLKC-ETKLNST 360

Query: 361 AEAADGFFKLEAIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDL 420
           ++  DGF K+E +K+PF  +W  +S   DDCR+ECLRNCSC AYA+ENGI CMLW   DL
Sbjct: 361 SDKEDGFLKVEMVKVPFLAEWSNSSASADDCRRECLRNCSCRAYAYENGIHCMLW-SGDL 420

Query: 421 IDIQKFESGGADLYLRMAYADLDH--TTTVDQVKDKKRIIIAIVLPG-TFIIFITIAIYF 480
           IDI++FES G DLYL MAYADLD    ++ D +K  K I + IVLP  TFIIF  IAIYF
Sbjct: 421 IDIKQFESNGTDLYLLMAYADLDKILISSTDDIKATKGITLIIVLPVITFIIFFVIAIYF 480

Query: 481 WWRLKTHKHEKKTSIKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLS 540
           +WR KT K EKK ++ SS     LK  R DD IGDE++LEELPLYDFE+LAIATNNFDLS
Sbjct: 481 FWRWKTRKQEKKKTMNSSINENTLK-LRMDDMIGDEVKLEELPLYDFEKLAIATNNFDLS 540

Query: 541 NQLGQGGFGPVYKGKLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCC 600
           N+LGQGGFGPVYKGKLLNGQEIA+KRLSR SNQGYEEFI EV+VISKLQHRNLV+LLGCC
Sbjct: 541 NKLGQGGFGPVYKGKLLNGQEIAVKRLSRASNQGYEEFINEVRVISKLQHRNLVRLLGCC 600

Query: 601 IEGEEKMLIYEYMANGSLDAMIFG 611
           IEG+EKMLIYEYM N SLDA+IFG
Sbjct: 601 IEGDEKMLIYEYMPNLSLDALIFG 616

BLAST of MS025001 vs. NCBI nr
Match: XP_038895960.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 [Benincasa hispida])

HSP 1 Score: 786.6 bits (2030), Expect = 1.6e-223
Identity = 393/614 (64.01%), Postives = 470/614 (76.55%), Query Frame = 0

Query: 4   QTDWRFPLRFLLLL------LSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNS 63
           Q +W F  R L LL       S C   SDTITST +IK PATI SN ++F+LG+FSPLNS
Sbjct: 5   QMNWNFSDRLLFLLSLITCFSSKCCFGSDTITSTEYIKYPATIISNATSFELGWFSPLNS 64

Query: 64  TRRYVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSP 123
           T +Y+GIW + +S++T+VWVAN+D+PLN+TSGIFTIS DGN+V+LD N+T +WSSNV+SP
Sbjct: 65  TTQYIGIWYHQVSLKTLVWVANKDTPLNNTSGIFTISNDGNVVILDENNTTIWSSNVTSP 124

Query: 124 LVNTSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWK 183
             NT+A+ILD+GNL+L+D  SGFVIWESF+HPSN+FL SMKLITNK   +++K+Q TSWK
Sbjct: 125 TANTTARILDSGNLVLEDPASGFVIWESFKHPSNLFLPSMKLITNK--KTQEKLQFTSWK 184

Query: 184 NPSDPSTGNFLLGLDVQFLPELVIWN-GRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLV 243
            PSDPS GNF L LDV  +PE VI N G NPYWRSGPWNG+ FIG PEMI VYL GFNL 
Sbjct: 185 TPSDPSKGNFSLSLDVLNIPEAVIRNYGGNPYWRSGPWNGHTFIGIPEMISVYLVGFNLA 244

Query: 244 IGDNTYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGAC 303
           I D  Y  S  Y + D   Y  + LS +GN EQ+YWD  +   +  WSA  T+CD+YG C
Sbjct: 245 IEDQAYYFSISYSNEDQLLYNMV-LSPEGNLEQQYWDPSKENWEVSWSAFRTECDYYGVC 304

Query: 304 GTAGICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGF 363
           G  G+C+   SPVCSC+ GFKPK E+EWN+GNWS GCVR TPL C ++S N T    DGF
Sbjct: 305 GVFGVCNANVSPVCSCLTGFKPKDEDEWNRGNWSNGCVRNTPLQCENSSRNNTRVEEDGF 364

Query: 364 FKLEAIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFE 423
            K+E +K+PF  +W  +S   DDC+QEC +NCSC+AYA+ENGIGCM+WRR DL D+QKFE
Sbjct: 365 LKVELVKVPFLAEWSNSSTSADDCKQECFQNCSCSAYAYENGIGCMIWRR-DLFDVQKFE 424

Query: 424 SGGADLYLRMAYADLDHTTTVDQVKDKKR-IIIAIVLPGTFIIFITIAIYFWWRLKTHKH 483
           S GA+L+LR+AYADL    T++ V+ K   IIIAIV+P T +IFI IAIYFWWR K  K+
Sbjct: 425 SLGANLHLRLAYADLQ---TINDVRRKSTGIIIAIVIPATLMIFI-IAIYFWWRWKAGKN 484

Query: 484 EKKTSIKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFG 543
           E       SKKGK LK  R DD IGD+ + EELPLYD+E LAIAT+NFDLSN+LGQGGFG
Sbjct: 485 E------YSKKGKRLKLRR-DDMIGDKSKFEELPLYDYENLAIATHNFDLSNKLGQGGFG 544

Query: 544 PVYKGKLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLI 603
           PVYKG+LL+GQEIAIKRLSR SNQGYEEFI EV VISKLQHRNLVQLLGCCIEGEEKMLI
Sbjct: 545 PVYKGRLLDGQEIAIKRLSRASNQGYEEFINEVIVISKLQHRNLVQLLGCCIEGEEKMLI 603

Query: 604 YEYMANGSLDAMIF 610
           YEY+ N SLDA IF
Sbjct: 605 YEYLPNLSLDAFIF 603

BLAST of MS025001 vs. ExPASy Swiss-Prot
Match: Q9SXB8 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 OS=Arabidopsis thaliana OX=3702 GN=At1g11330 PE=1 SV=3)

HSP 1 Score: 517.3 bits (1331), Expect = 2.3e-145
Identity = 287/622 (46.14%), Postives = 383/622 (61.58%), Query Frame = 0

Query: 12  RFLLLLLS--------FCYGRSDTITSTNFIKD--PATIASNGSTFKLGFFSPLNSTR-- 71
           RF+LLLL+         C+G  D IT ++ IKD    T+      F+ GFF+P+NST   
Sbjct: 10  RFVLLLLACTCLLSRRLCFG-EDRITFSSPIKDSESETLLCKSGIFRFGFFTPVNSTTRL 69

Query: 72  RYVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLV 131
           RYVGIW   I +QT+VWVAN+DSP+NDTSG+ +I +DGNL V DG + ++WS+NVS P+ 
Sbjct: 70  RYVGIWYEKIPIQTVVWVANKDSPINDTSGVISIYQDGNLAVTDGRNRLVWSTNVSVPVA 129

Query: 132 --NTSAQILDTGNLILQD-AVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSW 191
              T  Q++D+GNL+LQD   +G ++WESF+HP + F+  M L T  D  +   ++LTSW
Sbjct: 130 PNATWVQLMDSGNLMLQDNRNNGEILWESFKHPYDSFMPRMTLGT--DGRTGGNLKLTSW 189

Query: 192 KNPSDPSTGNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMID-VYLSGFNL 251
            +  DPSTGN+  G+     PEL+IW    P WRSGPWNG  FIG P M   ++L GFNL
Sbjct: 190 TSHDDPSTGNYTAGIAPFTFPELLIWKNNVPTWRSGPWNGQVFIGLPNMDSLLFLDGFNL 249

Query: 252 VIGDNTYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGA 311
              DN  T+S  Y + D   Y F +L  +G   QK W       +       T CD YG 
Sbjct: 250 -NSDNQGTISMSYAN-DSFMYHF-NLDPEGIIYQKDWSTSMRTWRIGVKFPYTDCDAYGR 309

Query: 312 CGTAGICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHN---SNNATAEA 371
           CG  G C    +P C C+KGF PK+  EWN GNWS GC+RK PL C      SN      
Sbjct: 310 CGRFGSCHAGENPPCKCVKGFVPKNNTEWNGGNWSNGCMRKAPLQCERQRNVSNGGGGGK 369

Query: 372 ADGFFKLEAIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDI 431
           ADGF KL+ +K+P   +  EAS  E  C + CL NCSCTAYA++ GIGCMLW   DL+D+
Sbjct: 370 ADGFLKLQKMKVPISAERSEAS--EQVCPKVCLDNCSCTAYAYDRGIGCMLW-SGDLVDM 429

Query: 432 QKFESGGADLYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKT 491
           Q F   G DL++R+A+++L         K    + + I  P   ++ I         L  
Sbjct: 430 QSFLGSGIDLFIRVAHSEL---------KTHSNLAVMIAAPVIGVMLIAAVCVL---LAC 489

Query: 492 HKHEKKTSIKSSKKGKIL-----KCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSN 551
            K++K+ +    +  +++       T D+++  ++I+L+ELPL++F+ LA +T++F L N
Sbjct: 490 RKYKKRPAPAKDRSAELMFKRMEALTSDNESASNQIKLKELPLFEFQVLATSTDSFSLRN 549

Query: 552 QLGQGGFGPVYKGKLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCI 610
           +LGQGGFGPVYKGKL  GQEIA+KRLSR S QG EE + EV VISKLQHRNLV+LLGCCI
Sbjct: 550 KLGQGGFGPVYKGKLPEGQEIAVKRLSRKSGQGLEELMNEVVVISKLQHRNLVKLLGCCI 609

BLAST of MS025001 vs. ExPASy Swiss-Prot
Match: Q9LPZ9 (G-type lectin S-receptor-like serine/threonine-protein kinase SD1-13 OS=Arabidopsis thaliana OX=3702 GN=SD113 PE=1 SV=2)

HSP 1 Score: 516.5 bits (1329), Expect = 4.0e-145
Identity = 269/608 (44.24%), Postives = 385/608 (63.32%), Query Frame = 0

Query: 14  LLLLLSFCYG-----RSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRRYVGIWLNN 73
           +LLL   C+       +D IT ++  +D  T+ SN STF+ GFFSP+NST RY GIW NN
Sbjct: 6   ILLLTLICFSLRLCLATDVITFSSEFRDSETVVSNHSTFRFGFFSPVNSTGRYAGIWFNN 65

Query: 74  ISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVNTS--AQIL 133
           I VQT+VWVAN +SP+ND+SG+ +ISK+GNLVV+DG   V WS+NV  P+   +  A++L
Sbjct: 66  IPVQTVVWVANSNSPINDSSGMVSISKEGNLVVMDGRGQVHWSTNVLVPVAANTFYARLL 125

Query: 134 DTGNLILQDAVS--GFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPSDPST 193
           +TGNL+L    +    ++WESF+HP N++L +M L T  D  +   ++L SWK+P DPS 
Sbjct: 126 NTGNLVLLGTTNTGDEILWESFEHPQNIYLPTMSLAT--DTKTGRSLKLRSWKSPFDPSP 185

Query: 194 GNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLVI-GDNTYT 253
           G +  GL     PELV+W      WRSGPWNG  FIG P M D  ++ F L +  DN  +
Sbjct: 186 GRYSAGLIPLPFPELVVWKDDLLMWRSGPWNGQYFIGLPNM-DYRINLFELTLSSDNRGS 245

Query: 254 LSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAGIC- 313
           +S  Y    +  Y FL L S+G+  Q+ W+    + K +     T+CD Y  CG    C 
Sbjct: 246 VSMSYAGNTLL-YHFL-LDSEGSVFQRDWNVAIQEWKTWLKVPSTKCDTYATCGQFASCR 305

Query: 314 -DVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLEA 373
            +  ++P C CI+GFKP+   EWN GNW++GCVRK PL C    NN  +  +DGF +++ 
Sbjct: 306 FNPGSTPPCMCIRGFKPQSYAEWNNGNWTQGCVRKAPLQCESRDNNDGSRKSDGFVRVQK 365

Query: 374 IKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGGAD 433
           +K+P   Q   +   E DC + CL+NCSCTAY+F+ GIGC+LW   +L+D+Q+F   G  
Sbjct: 366 MKVPHNPQ--RSGANEQDCPESCLKNCSCTAYSFDRGIGCLLW-SGNLMDMQEFSGTGVV 425

Query: 434 LYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTSI 493
            Y+R+A ++    T      ++  +I   +L G F+   T+ +   W++  H+ + + + 
Sbjct: 426 FYIRLADSEFKKRT------NRSIVITVTLLVGAFLFAGTVVLAL-WKIAKHREKNRNTR 485

Query: 494 KSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKGK 553
             +++ + L        + ++ +L+ELPL++F+ LA+ATNNF ++N+LGQGGFG VYKG+
Sbjct: 486 LLNERMEALSSNDVGAILVNQYKLKELPLFEFQVLAVATNNFSITNKLGQGGFGAVYKGR 545

Query: 554 LLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMAN 610
           L  G +IA+KRLSRTS QG EEF+ EV VISKLQHRNLV+LLG CIEGEE+ML+YE+M  
Sbjct: 546 LQEGLDIAVKRLSRTSGQGVEEFVNEVVVISKLQHRNLVRLLGFCIEGEERMLVYEFMPE 598

BLAST of MS025001 vs. ExPASy Swiss-Prot
Match: Q9SXB4 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11300 OS=Arabidopsis thaliana OX=3702 GN=At1g11300 PE=2 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 3.1e-137
Identity = 272/619 (43.94%), Postives = 378/619 (61.07%), Query Frame = 0

Query: 13  FLLLLLSFCYGRSDTITS-----TNFIKDPATIASNGSTFKLGFFSPLNSTRRYVGIWLN 72
           F+ +L+  C+  S ++       +  + D  TI S+  TF+ GFFSP+NST RY GIW N
Sbjct: 10  FVCILVLSCFFLSVSLAQERAFFSGKLNDSETIVSSFRTFRFGFFSPVNSTSRYAGIWYN 69

Query: 73  NISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLV--NTSAQI 132
           ++SVQT++WVAN+D P+ND+SG+ ++S+DGNLVV DG   VLWS+NVS+     +T A++
Sbjct: 70  SVSVQTVIWVANKDKPINDSSGVISVSQDGNLVVTDGQRRVLWSTNVSTQASANSTVAEL 129

Query: 133 LDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPSDPSTG 192
           LD+GNL+L++A S   +WESF++P++ +L +M + TN  +     V +TSWK+PSDPS G
Sbjct: 130 LDSGNLVLKEASSDAYLWESFKYPTDSWLPNMLVGTNARIGG-GNVTITSWKSPSDPSPG 189

Query: 193 NFLLGLDVQFLPELVIWNGRN---PYWRSGPWNGNAFIGRPEMIDVYLSGF--NLVIGDN 252
           ++   L +   PEL I N  N     WRSGPWNG  F G P   DVY   F    ++ D+
Sbjct: 190 SYTAALVLAAYPELFIMNNNNNNSTVWRSGPWNGQMFNGLP---DVYAGVFLYRFIVNDD 249

Query: 253 TY-TLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTA 312
           T  +++  Y +     Y ++    +G+  ++ W               T+CD Y  CG  
Sbjct: 250 TNGSVTMSYANDSTLRYFYMDY--RGSVIRRDWSETRRNWTVGLQVPATECDNYRRCGEF 309

Query: 313 GICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKL 372
             C+ + +P+CSCI+GF+P++  EWN GNWS GC R+ PL C   +NN    +ADGF +L
Sbjct: 310 ATCNPRKNPLCSCIRGFRPRNLIEWNNGNWSGGCTRRVPLQCERQNNNG---SADGFLRL 369

Query: 373 EAIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGG 432
             +KLP F +  EAS  E +C + CL+ CSC A A   G GCM+W    L+D Q+  + G
Sbjct: 370 RRMKLPDFARRSEAS--EPECLRTCLQTCSCIAAAHGLGYGCMIW-NGSLVDSQELSASG 429

Query: 433 ADLYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKT 492
            DLY+R+A++++       + KDK+ I+I  +L G   IF+  A     R    K   K 
Sbjct: 430 LDLYIRLAHSEI-------KTKDKRPILIGTILAGG--IFVVAACVLLARRIVMKKRAK- 489

Query: 493 SIKSSKKGKILKCTRDDDTIGDEIE---------LEELPLYDFEELAIATNNFDLSNQLG 552
                KKG      RD + I + +E         L+ELPL++F+ LA ATNNF L N+LG
Sbjct: 490 -----KKG------RDAEQIFERVEALAGGNKGKLKELPLFEFQVLAAATNNFSLRNKLG 549

Query: 553 QGGFGPVYKGKLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGE 610
           QGGFGPVYKGKL  GQEIA+KRLSR S QG EE + EV VISKLQHRNLV+LLGCCI GE
Sbjct: 550 QGGFGPVYKGKLQEGQEIAVKRLSRASGQGLEELVNEVVVISKLQHRNLVKLLGCCIAGE 595

BLAST of MS025001 vs. ExPASy Swiss-Prot
Match: Q9SXB5 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11303 OS=Arabidopsis thaliana OX=3702 GN=At1g11303 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 7.8e-133
Identity = 262/594 (44.11%), Postives = 363/594 (61.11%), Query Frame = 0

Query: 34  IKDPATIASNGSTFKLGFFSPLNSTRRYVGIWLNNISVQTIVWVANRDSPLNDTSGIFTI 93
           + D  TI S+  TF+ GFFSP+NST RY GIW N+I VQT++WVAN+D+P+ND+SG+ +I
Sbjct: 36  LNDSETIVSSFRTFRFGFFSPVNSTNRYAGIWYNSIPVQTVIWVANKDTPINDSSGVISI 95

Query: 94  SKDGNLVVLDGNDTVLWSSNVS--SPLVNTSAQILDTGNLILQDAVSGFVIWESFQHPSN 153
           S+DGNLVV DG   VLWS+NVS  +   +T A++L++GNL+L+DA +   +WESF++P++
Sbjct: 96  SEDGNLVVTDGQRRVLWSTNVSTRASANSTVAELLESGNLVLKDANTDAYLWESFKYPTD 155

Query: 154 VFLYSMKLITNKDMNSKDKVQLTSWKNPSDPSTGNFLLGLDVQFLPELVIWNGRN---PY 213
            +L +M + TN        + +TSW NPSDPS G++   L +   PEL I+N  +     
Sbjct: 156 SWLPNMLVGTNA-RTGGGNITITSWTNPSDPSPGSYTAALVLAPYPELFIFNNNDNNATV 215

Query: 214 WRSGPWNGNAFIGRPEMIDVY--LSGFNLVIGDNTYTLSTPYRSVDMQEYEFLSLSSQGN 273
           WRSGPWNG  F G P   DVY  L  +   + D+T   +T            +S ++   
Sbjct: 216 WRSGPWNGLMFNGLP---DVYPGLFLYRFKVNDDTNGSAT------------MSYANDST 275

Query: 274 WEQKYWDAKEAQLKNYWS------ALG-----TQCDFYGACGTAGICDVKASPVCSCIKG 333
               Y D +   ++  WS       LG     T+CD Y  CG    C+ + +P CSCIKG
Sbjct: 276 LRHLYLDYRGFAIRRDWSEARRNWTLGSQVPATECDIYSRCGQYTTCNPRKNPHCSCIKG 335

Query: 334 FKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLEAIKLPFFEQWYEASI 393
           F+P++  EWN GNWS GC+RK PL C   +N     +AD F KL+ +K+P F +  EAS 
Sbjct: 336 FRPRNLIEWNNGNWSGGCIRKLPLQCERQNNKG---SADRFLKLQRMKMPDFARRSEAS- 395

Query: 394 YEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGGADLYLRMAYADLDHTT 453
            E +C   CL++CSC A+A   G GCM+W R  L+D Q   + G DL +R+A+++     
Sbjct: 396 -EPECFMTCLQSCSCIAFAHGLGYGCMIWNR-SLVDSQVLSASGMDLSIRLAHSEF---- 455

Query: 454 TVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTSIKSSKKGKILKCTRD 513
              + +D++ I+I   L G   +  T  +    R+   K  KK    + +  K ++    
Sbjct: 456 ---KTQDRRPILIGTSLAGGIFVVAT-CVLLARRIVMKKRAKKKGTDAEQIFKRVEALAG 515

Query: 514 DDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKGKLLNGQEIAIKRLSR 573
               G   +L+ELPL++F+ LA AT+NF LSN+LGQGGFGPVYKG LL GQEIA+KRLS+
Sbjct: 516 ----GSREKLKELPLFEFQVLATATDNFSLSNKLGQGGFGPVYKGMLLEGQEIAVKRLSQ 575

Query: 574 TSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMANGSLDAMIF 610
            S QG EE +TEV VISKLQHRNLV+L GCCI GEE+ML+YE+M   SLD  IF
Sbjct: 576 ASGQGLEELVTEVVVISKLQHRNLVKLFGCCIAGEERMLVYEFMPKKSLDFYIF 595

BLAST of MS025001 vs. ExPASy Swiss-Prot
Match: O64781 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g61390 OS=Arabidopsis thaliana OX=3702 GN=At1g61390 PE=3 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 2.2e-127
Identity = 244/597 (40.87%), Postives = 362/597 (60.64%), Query Frame = 0

Query: 14  LLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRRYVGIWLNNISVQT 73
           LLL++   +G +D  TS+  +    T++S    ++LGFFSP NS ++YVGIW  NI+ Q 
Sbjct: 31  LLLIIFPTFGYADINTSSP-LSIGQTLSSPDGVYELGFFSPNNSRKQYVGIWFKNIAPQV 90

Query: 74  IVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVNTSAQILDTGNLIL 133
           +VWVANRD P+  T+   TIS +G+L++LDG   V+WS+  +       A++LDTGNL++
Sbjct: 91  VVWVANRDKPVTKTAANLTISSNGSLILLDGTQDVIWSTGEAFTSNKCHAELLDTGNLVV 150

Query: 134 QDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPSDPSTGNFLLGLDV 193
            D VSG  +W+SF++  N  L    ++ +     K++V LTSW++ SDPS G F L    
Sbjct: 151 IDDVSGKTLWKSFENLGNTMLPQSSVMYDIP-RGKNRV-LTSWRSNSDPSPGEFTLEFTP 210

Query: 194 QFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLVIGDNTYTLSTPYRSVDM 253
           Q  P+ +I  G +PYWRSGPW    F G P +   Y+S F ++      T S  Y  +  
Sbjct: 211 QVPPQGLIRRGSSPYWRSGPWAKTRFSGIPGIDASYVSPFTVLQDVAKGTASFSYSMLRN 270

Query: 254 QEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAGICDVKASPVCSCI 313
            +  +++L+S+G  +  + D K  +L  ++ A  + CD Y ACG  G+C    +P C C+
Sbjct: 271 YKLSYVTLTSEGKMKILWNDGKSWKL--HFEAPTSSCDLYRACGPFGLCVRSRNPKCICL 330

Query: 314 KGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNAT-AEAADGFFKLEAIKLPFFEQWYE 373
           KGF PK ++EW KGNW+ GCVR+T L+C  NS+  T  +  D F+ +  +K P   Q   
Sbjct: 331 KGFVPKSDDEWKKGNWTSGCVRRTQLSCHTNSSTKTQGKETDSFYHMTRVKTPDLYQ-LA 390

Query: 374 ASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGGADLYLRMAYADLD 433
             +  + C Q+CL NCSCTA+A+ +GIGC++W R +L+D  +F S G  L LR+A ++L 
Sbjct: 391 GFLNAEQCYQDCLGNCSCTAFAYISGIGCLVWNR-ELVDTVQFLSDGESLSLRLASSELA 450

Query: 434 HTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTSIKSSKKGKILKC 493
            +       ++ +II+   +  +  + +  A Y  WR +T ++E       S +    K 
Sbjct: 451 GS-------NRTKIILGTTVSLSIFVILVFAAYKSWRYRTKQNEPNPMFIHSSQDAWAKD 510

Query: 494 TRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKGKLLNGQEIAIKR 553
               D  G       + L+D   +  ATNNF  SN+LGQGGFGPVYKGKL++G+EIA+KR
Sbjct: 511 MEPQDVSG-------VNLFDMHTIRTATNNFSSSNKLGQGGFGPVYKGKLVDGKEIAVKR 570

Query: 554 LSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMANGSLDAMIF 610
           LS +S QG +EF+ E+++ISKLQH+NLV+LLGCCI+GEEK+LIYEY+ N SLD  +F
Sbjct: 571 LSSSSGQGTDEFMNEIRLISKLQHKNLVRLLGCCIKGEEKLLIYEYLVNKSLDVFLF 606

BLAST of MS025001 vs. ExPASy TrEMBL
Match: A0A6J1CDC3 (Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=LOC111010636 PE=3 SV=1)

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 590/609 (96.88%), Postives = 595/609 (97.70%), Query Frame = 0

Query: 1   MRLQTDWRFPLRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRR 60
           MRLQTDWRF LRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRR
Sbjct: 1   MRLQTDWRFSLRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRR 60

Query: 61  YVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVN 120
           YVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVN
Sbjct: 61  YVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVN 120

Query: 121 TSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPS 180
           TSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPS
Sbjct: 121 TSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPS 180

Query: 181 DPSTGNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLVIGDN 240
           DPSTGNFL GLDVQ+LPELVIWNGRNPYWRSGPWNGN FIGRPEM D+YLSG+NLVIGDN
Sbjct: 181 DPSTGNFLFGLDVQYLPELVIWNGRNPYWRSGPWNGNTFIGRPEMTDIYLSGYNLVIGDN 240

Query: 241 TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG 300
           TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG
Sbjct: 241 TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG 300

Query: 301 ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE 360
           ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE
Sbjct: 301 ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE 360

Query: 361 AIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGGA 420
            IKLPF EQW  AS YED+CRQEC  NCSCTAYAFE GIGCMLWRRDDLIDIQKFESGGA
Sbjct: 361 TIKLPFLEQWSNASYYEDECRQECFTNCSCTAYAFEKGIGCMLWRRDDLIDIQKFESGGA 420

Query: 421 DLYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTS 480
           DLYLRMAYADLDHTT  +QVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTS
Sbjct: 421 DLYLRMAYADLDHTT--NQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTS 480

Query: 481 IKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKG 540
           IKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKG
Sbjct: 481 IKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKG 540

Query: 541 KLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMA 600
           KLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMA
Sbjct: 541 KLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMA 600

Query: 601 NGSLDAMIF 610
           NGSLDAMIF
Sbjct: 601 NGSLDAMIF 607

BLAST of MS025001 vs. ExPASy TrEMBL
Match: A0A6J1CDU0 (G-type lectin S-receptor-like serine/threonine-protein kinase SD1-13 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010636 PE=4 SV=1)

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 590/609 (96.88%), Postives = 595/609 (97.70%), Query Frame = 0

Query: 1   MRLQTDWRFPLRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRR 60
           MRLQTDWRF LRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRR
Sbjct: 1   MRLQTDWRFSLRFLLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRR 60

Query: 61  YVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVN 120
           YVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVN
Sbjct: 61  YVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVN 120

Query: 121 TSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPS 180
           TSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPS
Sbjct: 121 TSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPS 180

Query: 181 DPSTGNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLVIGDN 240
           DPSTGNFL GLDVQ+LPELVIWNGRNPYWRSGPWNGN FIGRPEM D+YLSG+NLVIGDN
Sbjct: 181 DPSTGNFLFGLDVQYLPELVIWNGRNPYWRSGPWNGNTFIGRPEMTDIYLSGYNLVIGDN 240

Query: 241 TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG 300
           TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG
Sbjct: 241 TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG 300

Query: 301 ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE 360
           ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE
Sbjct: 301 ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE 360

Query: 361 AIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGGA 420
            IKLPF EQW  AS YED+CRQEC  NCSCTAYAFE GIGCMLWRRDDLIDIQKFESGGA
Sbjct: 361 TIKLPFLEQWSNASYYEDECRQECFTNCSCTAYAFEKGIGCMLWRRDDLIDIQKFESGGA 420

Query: 421 DLYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTS 480
           DLYLRMAYADLDHTT  +QVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTS
Sbjct: 421 DLYLRMAYADLDHTT--NQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTS 480

Query: 481 IKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKG 540
           IKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKG
Sbjct: 481 IKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKG 540

Query: 541 KLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMA 600
           KLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMA
Sbjct: 541 KLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMA 600

Query: 601 NGSLDAMIF 610
           NGSLDAMIF
Sbjct: 601 NGSLDAMIF 607

BLAST of MS025001 vs. ExPASy TrEMBL
Match: A0A6J1CEZ0 (LOW QUALITY PROTEIN: uncharacterized protein LOC111010891 OS=Momordica charantia OX=3673 GN=LOC111010891 PE=4 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.9e-246
Identity = 433/613 (70.64%), Postives = 503/613 (82.06%), Query Frame = 0

Query: 10  PLRFLLLLLSF-CYGRS-----DTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRRYVG 69
           PL  LLLLLSF C+  +     DTITSTNFIKDPATI+SN S+F+LGFF+P NSTRRYVG
Sbjct: 12  PLLHLLLLLSFTCFSSTFCSAKDTITSTNFIKDPATISSNSSSFELGFFAPPNSTRRYVG 71

Query: 70  IWLNNISVQTIVWVANRDSPLNDTS-GIFTISKDGNLVVLDGNDTVLWSSNV-SSPLVNT 129
           IW N +SVQT++WVANRD+PLN+TS GIFTISKDGNLVVLDGNDTVLWSS+V SS   N 
Sbjct: 72  IWFNQVSVQTLIWVANRDNPLNNTSQGIFTISKDGNLVVLDGNDTVLWSSDVSSSSSTNR 131

Query: 130 SAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNK-DMNSKDKVQLTSWKNPS 189
           SA+ILD+GNL+L+DA SG VIW+SF+HPS+ FL +M++ITNK D+NSKDKVQLTSWKNPS
Sbjct: 132 SARILDSGNLVLEDASSGEVIWQSFKHPSDKFLPAMQIITNKLDVNSKDKVQLTSWKNPS 191

Query: 190 DPSTGNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLVIGDN 249
           DPSTGNF  G+DVQ LPE+V+WNGR+PYWRSGPWNGN+FIG PEM  VYLSG++LVI D 
Sbjct: 192 DPSTGNFSFGMDVQNLPEVVVWNGRDPYWRSGPWNGNSFIGVPEMEAVYLSGYSLVIQDQ 251

Query: 250 TYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAG 309
           TYTLS  Y +  +QE+ +L LS  GN +Q YWD  E + K  W +L T+CD YGACG  G
Sbjct: 252 TYTLSVSY-NYSIQEFAYLFLSPGGNLQQTYWDVSEERWKITWMSLQTRCDLYGACGAFG 311

Query: 310 ICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLE 369
           IC+ K SPVCSC+KGFKP HEEEWN+GNWS GCVR TPL C     NAT    DGF K+E
Sbjct: 312 ICNPKPSPVCSCLKGFKPNHEEEWNQGNWSGGCVRNTPLLC-----NATT-LEDGFLKVE 371

Query: 370 AIKLPFFEQWYE-ASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGG 429
            +KLPF  +W   +S+  DDCRQ CL+NCSCTAYA+ENGI CMLWRRDDLID+QKFESGG
Sbjct: 372 TVKLPFLAEWSGISSLTADDCRQLCLKNCSCTAYAYENGINCMLWRRDDLIDMQKFESGG 431

Query: 430 ADLYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFW--WRLKTHKHEK 489
           ADLY+RMAYADLDHTT  D+VKDK  IIIAIVLP T +IF+ IAIY W  W+ K  K+EK
Sbjct: 432 ADLYVRMAYADLDHTTN-DRVKDKTGIIIAIVLPTTLVIFV-IAIYLWLRWKRKAPKNEK 491

Query: 490 KTSIKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPV 549
           K ++ S +K KILK  R+DD I D+I+LEELP+YD E+LA+ATNNFDL+N+LGQGGFGPV
Sbjct: 492 KKTMASGEKEKILKLKREDDMIEDDIKLEELPIYDLEKLAMATNNFDLTNKLGQGGFGPV 551

Query: 550 YKGKLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYE 609
           YKGKL NGQEIA+KRLSR S QGYEEFI EV+VISKLQHRNLV+L GCCIEGEEKMLIYE
Sbjct: 552 YKGKLANGQEIAVKRLSRVSQQGYEEFINEVRVISKLQHRNLVRLFGCCIEGEEKMLIYE 611

Query: 610 YMANGSLDAMIFG 611
           YM N SLDA+IFG
Sbjct: 612 YMPNLSLDALIFG 615

BLAST of MS025001 vs. ExPASy TrEMBL
Match: A0A6J1CG12 (uncharacterized protein LOC111010889 OS=Momordica charantia OX=3673 GN=LOC111010889 PE=4 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 3.2e-230
Identity = 414/624 (66.35%), Postives = 479/624 (76.76%), Query Frame = 0

Query: 1   MRLQTDWRFPLRFLLLL--------LSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFF 60
           M+ QT+W F  R LL+L        L  C GR DTITSTNFIKDP TI SN S+F LGFF
Sbjct: 1   MKPQTNWSFSCRLLLILSFTCFSSRLLICSGR-DTITSTNFIKDPETITSNASSFHLGFF 60

Query: 61  SPLNSTRRYVGIW-LNNISVQTIVWVANRDSPLNDTS-GIFTISKDGNLVVLDGNDTVLW 120
           +P NSTRRYVGIW +N I  QT+VWVANRD+PLN+TS GIFTISKDGNLVVLDGN+TVLW
Sbjct: 61  TPPNSTRRYVGIWFINQIPQQTVVWVANRDNPLNNTSQGIFTISKDGNLVVLDGNNTVLW 120

Query: 121 SSNVSSPLVNTSAQILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDK 180
           SSNVSSP  N SA+ILD+GNL+L+DA SG VIWESF+HPS+ FL SMKLITN    +++K
Sbjct: 121 SSNVSSPATNRSARILDSGNLVLEDASSGEVIWESFKHPSDKFLTSMKLITN--AKTREK 180

Query: 181 VQLTSWKNPSDPSTGNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYL 240
           V+LTSW  PSDPSTGNF LG+ V  +PE VIW GRN YWRSGPWNG  FIG PEM   YL
Sbjct: 181 VELTSWNTPSDPSTGNFSLGIYVHNIPEAVIWKGRNTYWRSGPWNGQNFIGIPEMDSTYL 240

Query: 241 SGFNLVIGDNTYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDA-KEAQLKNYWSALGTQ 300
           SG+ L I D +Y  S  Y   D +++ +L LSSQGN  +   D   E + +  WSAL TQ
Sbjct: 241 SGYKLTIEDQSYHFSVAYN--DDEQFGYLFLSSQGNLVETNVDLDDERRWRATWSALQTQ 300

Query: 301 CDFYGACGTAGICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNAT 360
           CD YG CG  GICD KASP+CSC++GFKP HE++WN+GNWS GCVRKTPL C     N+T
Sbjct: 301 CDIYGTCGAFGICDAKASPICSCLRGFKPNHEQDWNRGNWSGGCVRKTPLKC-ETKLNST 360

Query: 361 AEAADGFFKLEAIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDL 420
           ++  DGF K+E +K+PF  +W  +S   DDCR+ECLRNCSC AYA+ENGI CMLW   DL
Sbjct: 361 SDKEDGFLKVEMVKVPFLAEWSNSSASADDCRRECLRNCSCRAYAYENGIHCMLW-SGDL 420

Query: 421 IDIQKFESGGADLYLRMAYADLDH--TTTVDQVKDKKRIIIAIVLPG-TFIIFITIAIYF 480
           IDI++FES G DLYL MAYADLD    ++ D +K  K I + IVLP  TFIIF  IAIYF
Sbjct: 421 IDIKQFESNGTDLYLLMAYADLDKILISSTDDIKATKGITLIIVLPVITFIIFFVIAIYF 480

Query: 481 WWRLKTHKHEKKTSIKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLS 540
           +WR KT K EKK ++ SS     LK  R DD IGDE++LEELPLYDFE+LAIATNNFDLS
Sbjct: 481 FWRWKTRKQEKKKTMNSSINENTLK-LRMDDMIGDEVKLEELPLYDFEKLAIATNNFDLS 540

Query: 541 NQLGQGGFGPVYKGKLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCC 600
           N+LGQGGFGPVYKGKLLNGQEIA+KRLSR SNQGYEEFI EV+VISKLQHRNLV+LLGCC
Sbjct: 541 NKLGQGGFGPVYKGKLLNGQEIAVKRLSRASNQGYEEFINEVRVISKLQHRNLVRLLGCC 600

Query: 601 IEGEEKMLIYEYMANGSLDAMIFG 611
           IEG+EKMLIYEYM N SLDA+IFG
Sbjct: 601 IEGDEKMLIYEYMPNLSLDALIFG 616

BLAST of MS025001 vs. ExPASy TrEMBL
Match: A0A6J1EHX5 (uncharacterized protein LOC111434316 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434316 PE=4 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 7.9e-221
Identity = 396/607 (65.24%), Postives = 470/607 (77.43%), Query Frame = 0

Query: 11   LRFLLLLLSFC--YG-RSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRRYVGIWLN 70
            L  LL L SFC  +G   DTITS NFI+DPATI SNGS F+LGFFSP+NSTRRYVGIW  
Sbjct: 841  LLLLLSLTSFCSRFGFAGDTITSANFIEDPATILSNGSVFELGFFSPVNSTRRYVGIWFQ 900

Query: 71   NISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLV---NTSAQ 130
              S QTIVWVANRD+P+ DTSGIFTISKDGNLVVLD ND++LWSSNVSS ++   NTSAQ
Sbjct: 901  EFSPQTIVWVANRDNPVKDTSGIFTISKDGNLVVLDSNDSILWSSNVSSSVIGTDNTSAQ 960

Query: 131  ILDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPSDPST 190
            ILD+GNL+L+D+ SG +IWESF+HP + F   MK+ TN    +K+ V  TSW  PSDPST
Sbjct: 961  ILDSGNLVLKDSTSGVIIWESFKHPCDKFWTPMKIKTN--TRTKEVVGFTSWNTPSDPST 1020

Query: 191  GNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLVIGDNTYTL 250
            G F   LDV  LPE VI NG + YWRSGPWNG +FIG PEM  VYLSG+NL I D TYTL
Sbjct: 1021 GKFSFLLDVHDLPEAVILNGGDTYWRSGPWNGQSFIGVPEMNSVYLSGYNLAIEDQTYTL 1080

Query: 251  STPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAGICDV 310
            S   +    +E+ +L L+SQGN EQ  WD+++      W AL T+CDFYGACG  GIC+ 
Sbjct: 1081 SLASKYA-FREFSYLFLNSQGNVEQMNWDSEKQYWNFSWLALKTECDFYGACGAFGICNA 1140

Query: 311  KASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLEAIKL 370
            K SPVCSC++GF+PKHEEEWN+GNWS GCVRKTPL C     N ++   DGFFKLE +K+
Sbjct: 1141 KTSPVCSCLRGFEPKHEEEWNRGNWSNGCVRKTPLKC----ENRSSTEEDGFFKLEMVKV 1200

Query: 371  PFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGGADLYL 430
            PF  +W  +S   DDCR++CL NC C++YAFEN I CM W R+DLID+QKFESGG DL+L
Sbjct: 1201 PFLAEWSNSSASVDDCRRDCLENCWCSSYAFENEI-CMHW-RNDLIDMQKFESGGVDLHL 1260

Query: 431  RMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTSIKSS 490
            RMA ADLD     + V+DKKR+IIA+V+P T +IFI IAI F+W+ KT K +KK  + S 
Sbjct: 1261 RMALADLD----TNNVRDKKRVIIAVVVPATLVIFI-IAIAFYWKWKTKKQKKKIMMTSG 1320

Query: 491  KKGKILKCTRDDDT-IGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKGKLL 550
            +K K +K TR++DT I D+I+LEELPLYDFE++AIATN FD+SN+LGQGGFGPVYKG+LL
Sbjct: 1321 EKEK-MKQTRENDTMIEDDIKLEELPLYDFEKVAIATNYFDMSNKLGQGGFGPVYKGRLL 1380

Query: 551  NGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMANGS 610
            NGQEIA+KRLSR S QGYEEFI EV+VISKLQHRNLV+LLGCCIEGEEKMLIYEYM N S
Sbjct: 1381 NGQEIAVKRLSRASKQGYEEFINEVRVISKLQHRNLVRLLGCCIEGEEKMLIYEYMPNLS 1432

BLAST of MS025001 vs. TAIR 10
Match: AT1G11330.2 (S-locus lectin protein kinase family protein )

HSP 1 Score: 517.3 bits (1331), Expect = 1.7e-146
Identity = 287/622 (46.14%), Postives = 383/622 (61.58%), Query Frame = 0

Query: 12  RFLLLLLS--------FCYGRSDTITSTNFIKD--PATIASNGSTFKLGFFSPLNSTR-- 71
           RF+LLLL+         C+G  D IT ++ IKD    T+      F+ GFF+P+NST   
Sbjct: 10  RFVLLLLACTCLLSRRLCFG-EDRITFSSPIKDSESETLLCKSGIFRFGFFTPVNSTTRL 69

Query: 72  RYVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLV 131
           RYVGIW   I +QT+VWVAN+DSP+NDTSG+ +I +DGNL V DG + ++WS+NVS P+ 
Sbjct: 70  RYVGIWYEKIPIQTVVWVANKDSPINDTSGVISIYQDGNLAVTDGRNRLVWSTNVSVPVA 129

Query: 132 --NTSAQILDTGNLILQD-AVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSW 191
              T  Q++D+GNL+LQD   +G ++WESF+HP + F+  M L T  D  +   ++LTSW
Sbjct: 130 PNATWVQLMDSGNLMLQDNRNNGEILWESFKHPYDSFMPRMTLGT--DGRTGGNLKLTSW 189

Query: 192 KNPSDPSTGNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMID-VYLSGFNL 251
            +  DPSTGN+  G+     PEL+IW    P WRSGPWNG  FIG P M   ++L GFNL
Sbjct: 190 TSHDDPSTGNYTAGIAPFTFPELLIWKNNVPTWRSGPWNGQVFIGLPNMDSLLFLDGFNL 249

Query: 252 VIGDNTYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGA 311
              DN  T+S  Y + D   Y F +L  +G   QK W       +       T CD YG 
Sbjct: 250 -NSDNQGTISMSYAN-DSFMYHF-NLDPEGIIYQKDWSTSMRTWRIGVKFPYTDCDAYGR 309

Query: 312 CGTAGICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHN---SNNATAEA 371
           CG  G C    +P C C+KGF PK+  EWN GNWS GC+RK PL C      SN      
Sbjct: 310 CGRFGSCHAGENPPCKCVKGFVPKNNTEWNGGNWSNGCMRKAPLQCERQRNVSNGGGGGK 369

Query: 372 ADGFFKLEAIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDI 431
           ADGF KL+ +K+P   +  EAS  E  C + CL NCSCTAYA++ GIGCMLW   DL+D+
Sbjct: 370 ADGFLKLQKMKVPISAERSEAS--EQVCPKVCLDNCSCTAYAYDRGIGCMLW-SGDLVDM 429

Query: 432 QKFESGGADLYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKT 491
           Q F   G DL++R+A+++L         K    + + I  P   ++ I         L  
Sbjct: 430 QSFLGSGIDLFIRVAHSEL---------KTHSNLAVMIAAPVIGVMLIAAVCVL---LAC 489

Query: 492 HKHEKKTSIKSSKKGKIL-----KCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSN 551
            K++K+ +    +  +++       T D+++  ++I+L+ELPL++F+ LA +T++F L N
Sbjct: 490 RKYKKRPAPAKDRSAELMFKRMEALTSDNESASNQIKLKELPLFEFQVLATSTDSFSLRN 549

Query: 552 QLGQGGFGPVYKGKLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCI 610
           +LGQGGFGPVYKGKL  GQEIA+KRLSR S QG EE + EV VISKLQHRNLV+LLGCCI
Sbjct: 550 KLGQGGFGPVYKGKLPEGQEIAVKRLSRKSGQGLEELMNEVVVISKLQHRNLVKLLGCCI 609

BLAST of MS025001 vs. TAIR 10
Match: AT1G11350.1 (S-domain-1 13 )

HSP 1 Score: 516.5 bits (1329), Expect = 2.8e-146
Identity = 269/608 (44.24%), Postives = 385/608 (63.32%), Query Frame = 0

Query: 14  LLLLLSFCYG-----RSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRRYVGIWLNN 73
           +LLL   C+       +D IT ++  +D  T+ SN STF+ GFFSP+NST RY GIW NN
Sbjct: 6   ILLLTLICFSLRLCLATDVITFSSEFRDSETVVSNHSTFRFGFFSPVNSTGRYAGIWFNN 65

Query: 74  ISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVNTS--AQIL 133
           I VQT+VWVAN +SP+ND+SG+ +ISK+GNLVV+DG   V WS+NV  P+   +  A++L
Sbjct: 66  IPVQTVVWVANSNSPINDSSGMVSISKEGNLVVMDGRGQVHWSTNVLVPVAANTFYARLL 125

Query: 134 DTGNLILQDAVS--GFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPSDPST 193
           +TGNL+L    +    ++WESF+HP N++L +M L T  D  +   ++L SWK+P DPS 
Sbjct: 126 NTGNLVLLGTTNTGDEILWESFEHPQNIYLPTMSLAT--DTKTGRSLKLRSWKSPFDPSP 185

Query: 194 GNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLVI-GDNTYT 253
           G +  GL     PELV+W      WRSGPWNG  FIG P M D  ++ F L +  DN  +
Sbjct: 186 GRYSAGLIPLPFPELVVWKDDLLMWRSGPWNGQYFIGLPNM-DYRINLFELTLSSDNRGS 245

Query: 254 LSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAGIC- 313
           +S  Y    +  Y FL L S+G+  Q+ W+    + K +     T+CD Y  CG    C 
Sbjct: 246 VSMSYAGNTLL-YHFL-LDSEGSVFQRDWNVAIQEWKTWLKVPSTKCDTYATCGQFASCR 305

Query: 314 -DVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKLEA 373
            +  ++P C CI+GFKP+   EWN GNW++GCVRK PL C    NN  +  +DGF +++ 
Sbjct: 306 FNPGSTPPCMCIRGFKPQSYAEWNNGNWTQGCVRKAPLQCESRDNNDGSRKSDGFVRVQK 365

Query: 374 IKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGGAD 433
           +K+P   Q   +   E DC + CL+NCSCTAY+F+ GIGC+LW   +L+D+Q+F   G  
Sbjct: 366 MKVPHNPQ--RSGANEQDCPESCLKNCSCTAYSFDRGIGCLLW-SGNLMDMQEFSGTGVV 425

Query: 434 LYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTSI 493
            Y+R+A ++    T      ++  +I   +L G F+   T+ +   W++  H+ + + + 
Sbjct: 426 FYIRLADSEFKKRT------NRSIVITVTLLVGAFLFAGTVVLAL-WKIAKHREKNRNTR 485

Query: 494 KSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKGK 553
             +++ + L        + ++ +L+ELPL++F+ LA+ATNNF ++N+LGQGGFG VYKG+
Sbjct: 486 LLNERMEALSSNDVGAILVNQYKLKELPLFEFQVLAVATNNFSITNKLGQGGFGAVYKGR 545

Query: 554 LLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMAN 610
           L  G +IA+KRLSRTS QG EEF+ EV VISKLQHRNLV+LLG CIEGEE+ML+YE+M  
Sbjct: 546 LQEGLDIAVKRLSRTSGQGVEEFVNEVVVISKLQHRNLVRLLGFCIEGEERMLVYEFMPE 598

BLAST of MS025001 vs. TAIR 10
Match: AT1G11330.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 516.5 bits (1329), Expect = 2.8e-146
Identity = 287/617 (46.52%), Postives = 384/617 (62.24%), Query Frame = 0

Query: 12  RFLLLLLS--------FCYGRSDTITSTNFIKD--PATIASNGSTFKLGFFSPLNSTR-- 71
           RF+LLLL+         C+G  D IT ++ IKD    T+      F+ GFF+P+NST   
Sbjct: 10  RFVLLLLACTCLLSRRLCFG-EDRITFSSPIKDSESETLLCKSGIFRFGFFTPVNSTTRL 69

Query: 72  RYVGIWLNNISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLV 131
           RYVGIW   I +QT+VWVAN+DSP+NDTSG+ +I +DGNL V DG + ++WS+NVS P+ 
Sbjct: 70  RYVGIWYEKIPIQTVVWVANKDSPINDTSGVISIYQDGNLAVTDGRNRLVWSTNVSVPVA 129

Query: 132 --NTSAQILDTGNLILQD-AVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSW 191
              T  Q++D+GNL+LQD   +G ++WESF+HP + F+  M L T  D  +   ++LTSW
Sbjct: 130 PNATWVQLMDSGNLMLQDNRNNGEILWESFKHPYDSFMPRMTLGT--DGRTGGNLKLTSW 189

Query: 192 KNPSDPSTGNFLLGLDVQFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMID-VYLSGFNL 251
            +  DPSTGN+  G+     PEL+IW    P WRSGPWNG  FIG P M   ++L GFNL
Sbjct: 190 TSHDDPSTGNYTAGIAPFTFPELLIWKNNVPTWRSGPWNGQVFIGLPNMDSLLFLDGFNL 249

Query: 252 VIGDNTYTLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGA 311
              DN  T+S  Y + D   Y F +L  +G   QK W       +       T CD YG 
Sbjct: 250 -NSDNQGTISMSYAN-DSFMYHF-NLDPEGIIYQKDWSTSMRTWRIGVKFPYTDCDAYGR 309

Query: 312 CGTAGICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHN---SNNATAEA 371
           CG  G C    +P C C+KGF PK+  EWN GNWS GC+RK PL C      SN      
Sbjct: 310 CGRFGSCHAGENPPCKCVKGFVPKNNTEWNGGNWSNGCMRKAPLQCERQRNVSNGGGGGK 369

Query: 372 ADGFFKLEAIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDI 431
           ADGF KL+ +K+P   +  EAS  E  C + CL NCSCTAYA++ GIGCMLW   DL+D+
Sbjct: 370 ADGFLKLQKMKVPISAERSEAS--EQVCPKVCLDNCSCTAYAYDRGIGCMLW-SGDLVDM 429

Query: 432 QKFESGGADLYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKT 491
           Q F   G DL++R+A+++L       +      ++IA  + G  +I     +    + K 
Sbjct: 430 QSFLGSGIDLFIRVAHSEL-------KTHSNLAVMIAAPVIGVMLIAAVCVLLACRKYKK 489

Query: 492 HKHEKKTSIKSSKKGKILKCTRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQG 551
              + +++    K+ + L  T D+++  ++I+L+ELPL++F+ LA +T++F L N+LGQG
Sbjct: 490 RPAKDRSAELMFKRMEAL--TSDNESASNQIKLKELPLFEFQVLATSTDSFSLRNKLGQG 549

Query: 552 GFGPVYKGKLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEK 610
           GFGPVYKGKL  GQEIA+KRLSR S QG EE + EV VISKLQHRNLV+LLGCCIEGEE+
Sbjct: 550 GFGPVYKGKLPEGQEIAVKRLSRKSGQGLEELMNEVVVISKLQHRNLVKLLGCCIEGEER 608

BLAST of MS025001 vs. TAIR 10
Match: AT1G11300.1 (protein serine/threonine kinases;protein kinases;ATP binding;sugar binding;kinases;carbohydrate binding )

HSP 1 Score: 490.3 bits (1261), Expect = 2.2e-138
Identity = 272/619 (43.94%), Postives = 378/619 (61.07%), Query Frame = 0

Query: 13  FLLLLLSFCYGRSDTITS-----TNFIKDPATIASNGSTFKLGFFSPLNSTRRYVGIWLN 72
           F+ +L+  C+  S ++       +  + D  TI S+  TF+ GFFSP+NST RY GIW N
Sbjct: 10  FVCILVLSCFFLSVSLAQERAFFSGKLNDSETIVSSFRTFRFGFFSPVNSTSRYAGIWYN 69

Query: 73  NISVQTIVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLV--NTSAQI 132
           ++SVQT++WVAN+D P+ND+SG+ ++S+DGNLVV DG   VLWS+NVS+     +T A++
Sbjct: 70  SVSVQTVIWVANKDKPINDSSGVISVSQDGNLVVTDGQRRVLWSTNVSTQASANSTVAEL 129

Query: 133 LDTGNLILQDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPSDPSTG 192
           LD+GNL+L++A S   +WESF++P++ +L +M + TN  +     V +TSWK+PSDPS G
Sbjct: 130 LDSGNLVLKEASSDAYLWESFKYPTDSWLPNMLVGTNARIGG-GNVTITSWKSPSDPSPG 189

Query: 193 NFLLGLDVQFLPELVIWNGRN---PYWRSGPWNGNAFIGRPEMIDVYLSGF--NLVIGDN 252
           ++   L +   PEL I N  N     WRSGPWNG  F G P   DVY   F    ++ D+
Sbjct: 190 SYTAALVLAAYPELFIMNNNNNNSTVWRSGPWNGQMFNGLP---DVYAGVFLYRFIVNDD 249

Query: 253 TY-TLSTPYRSVDMQEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTA 312
           T  +++  Y +     Y ++    +G+  ++ W               T+CD Y  CG  
Sbjct: 250 TNGSVTMSYANDSTLRYFYMDY--RGSVIRRDWSETRRNWTVGLQVPATECDNYRRCGEF 309

Query: 313 GICDVKASPVCSCIKGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNATAEAADGFFKL 372
             C+ + +P+CSCI+GF+P++  EWN GNWS GC R+ PL C   +NN    +ADGF +L
Sbjct: 310 ATCNPRKNPLCSCIRGFRPRNLIEWNNGNWSGGCTRRVPLQCERQNNNG---SADGFLRL 369

Query: 373 EAIKLPFFEQWYEASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGG 432
             +KLP F +  EAS  E +C + CL+ CSC A A   G GCM+W    L+D Q+  + G
Sbjct: 370 RRMKLPDFARRSEAS--EPECLRTCLQTCSCIAAAHGLGYGCMIW-NGSLVDSQELSASG 429

Query: 433 ADLYLRMAYADLDHTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKT 492
            DLY+R+A++++       + KDK+ I+I  +L G   IF+  A     R    K   K 
Sbjct: 430 LDLYIRLAHSEI-------KTKDKRPILIGTILAGG--IFVVAACVLLARRIVMKKRAK- 489

Query: 493 SIKSSKKGKILKCTRDDDTIGDEIE---------LEELPLYDFEELAIATNNFDLSNQLG 552
                KKG      RD + I + +E         L+ELPL++F+ LA ATNNF L N+LG
Sbjct: 490 -----KKG------RDAEQIFERVEALAGGNKGKLKELPLFEFQVLAAATNNFSLRNKLG 549

Query: 553 QGGFGPVYKGKLLNGQEIAIKRLSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGE 610
           QGGFGPVYKGKL  GQEIA+KRLSR S QG EE + EV VISKLQHRNLV+LLGCCI GE
Sbjct: 550 QGGFGPVYKGKLQEGQEIAVKRLSRASGQGLEELVNEVVVISKLQHRNLVKLLGCCIAGE 595

BLAST of MS025001 vs. TAIR 10
Match: AT1G61390.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 457.6 bits (1176), Expect = 1.6e-128
Identity = 244/597 (40.87%), Postives = 362/597 (60.64%), Query Frame = 0

Query: 14  LLLLLSFCYGRSDTITSTNFIKDPATIASNGSTFKLGFFSPLNSTRRYVGIWLNNISVQT 73
           LLL++   +G +D  TS+  +    T++S    ++LGFFSP NS ++YVGIW  NI+ Q 
Sbjct: 31  LLLIIFPTFGYADINTSSP-LSIGQTLSSPDGVYELGFFSPNNSRKQYVGIWFKNIAPQV 90

Query: 74  IVWVANRDSPLNDTSGIFTISKDGNLVVLDGNDTVLWSSNVSSPLVNTSAQILDTGNLIL 133
           +VWVANRD P+  T+   TIS +G+L++LDG   V+WS+  +       A++LDTGNL++
Sbjct: 91  VVWVANRDKPVTKTAANLTISSNGSLILLDGTQDVIWSTGEAFTSNKCHAELLDTGNLVV 150

Query: 134 QDAVSGFVIWESFQHPSNVFLYSMKLITNKDMNSKDKVQLTSWKNPSDPSTGNFLLGLDV 193
            D VSG  +W+SF++  N  L    ++ +     K++V LTSW++ SDPS G F L    
Sbjct: 151 IDDVSGKTLWKSFENLGNTMLPQSSVMYDIP-RGKNRV-LTSWRSNSDPSPGEFTLEFTP 210

Query: 194 QFLPELVIWNGRNPYWRSGPWNGNAFIGRPEMIDVYLSGFNLVIGDNTYTLSTPYRSVDM 253
           Q  P+ +I  G +PYWRSGPW    F G P +   Y+S F ++      T S  Y  +  
Sbjct: 211 QVPPQGLIRRGSSPYWRSGPWAKTRFSGIPGIDASYVSPFTVLQDVAKGTASFSYSMLRN 270

Query: 254 QEYEFLSLSSQGNWEQKYWDAKEAQLKNYWSALGTQCDFYGACGTAGICDVKASPVCSCI 313
            +  +++L+S+G  +  + D K  +L  ++ A  + CD Y ACG  G+C    +P C C+
Sbjct: 271 YKLSYVTLTSEGKMKILWNDGKSWKL--HFEAPTSSCDLYRACGPFGLCVRSRNPKCICL 330

Query: 314 KGFKPKHEEEWNKGNWSRGCVRKTPLNCTHNSNNAT-AEAADGFFKLEAIKLPFFEQWYE 373
           KGF PK ++EW KGNW+ GCVR+T L+C  NS+  T  +  D F+ +  +K P   Q   
Sbjct: 331 KGFVPKSDDEWKKGNWTSGCVRRTQLSCHTNSSTKTQGKETDSFYHMTRVKTPDLYQ-LA 390

Query: 374 ASIYEDDCRQECLRNCSCTAYAFENGIGCMLWRRDDLIDIQKFESGGADLYLRMAYADLD 433
             +  + C Q+CL NCSCTA+A+ +GIGC++W R +L+D  +F S G  L LR+A ++L 
Sbjct: 391 GFLNAEQCYQDCLGNCSCTAFAYISGIGCLVWNR-ELVDTVQFLSDGESLSLRLASSELA 450

Query: 434 HTTTVDQVKDKKRIIIAIVLPGTFIIFITIAIYFWWRLKTHKHEKKTSIKSSKKGKILKC 493
            +       ++ +II+   +  +  + +  A Y  WR +T ++E       S +    K 
Sbjct: 451 GS-------NRTKIILGTTVSLSIFVILVFAAYKSWRYRTKQNEPNPMFIHSSQDAWAKD 510

Query: 494 TRDDDTIGDEIELEELPLYDFEELAIATNNFDLSNQLGQGGFGPVYKGKLLNGQEIAIKR 553
               D  G       + L+D   +  ATNNF  SN+LGQGGFGPVYKGKL++G+EIA+KR
Sbjct: 511 MEPQDVSG-------VNLFDMHTIRTATNNFSSSNKLGQGGFGPVYKGKLVDGKEIAVKR 570

Query: 554 LSRTSNQGYEEFITEVKVISKLQHRNLVQLLGCCIEGEEKMLIYEYMANGSLDAMIF 610
           LS +S QG +EF+ E+++ISKLQH+NLV+LLGCCI+GEEK+LIYEY+ N SLD  +F
Sbjct: 571 LSSSSGQGTDEFMNEIRLISKLQHKNLVRLLGCCIKGEEKLLIYEYLVNKSLDVFLF 606

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022139811.10.0e+0096.88G-type lectin S-receptor-like serine/threonine-protein kinase SD1-13 isoform X2 ... [more]
XP_022139810.10.0e+0096.88G-type lectin S-receptor-like serine/threonine-protein kinase SD1-13 isoform X1 ... [more]
XP_022140159.13.9e-24670.64LOW QUALITY PROTEIN: uncharacterized protein LOC111010891 [Momordica charantia][more]
XP_022140157.16.6e-23066.35uncharacterized protein LOC111010889 [Momordica charantia][more]
XP_038895960.11.6e-22364.01G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 [Beninca... [more]
Match NameE-valueIdentityDescription
Q9SXB82.3e-14546.14G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 OS=Arabi... [more]
Q9LPZ94.0e-14544.24G-type lectin S-receptor-like serine/threonine-protein kinase SD1-13 OS=Arabidop... [more]
Q9SXB43.1e-13743.94G-type lectin S-receptor-like serine/threonine-protein kinase At1g11300 OS=Arabi... [more]
Q9SXB57.8e-13344.11G-type lectin S-receptor-like serine/threonine-protein kinase At1g11303 OS=Arabi... [more]
O647812.2e-12740.87G-type lectin S-receptor-like serine/threonine-protein kinase At1g61390 OS=Arabi... [more]
Match NameE-valueIdentityDescription
A0A6J1CDC30.0e+0096.88Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1CDU00.0e+0096.88G-type lectin S-receptor-like serine/threonine-protein kinase SD1-13 isoform X2 ... [more]
A0A6J1CEZ01.9e-24670.64LOW QUALITY PROTEIN: uncharacterized protein LOC111010891 OS=Momordica charantia... [more]
A0A6J1CG123.2e-23066.35uncharacterized protein LOC111010889 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1EHX57.9e-22165.24uncharacterized protein LOC111434316 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT1G11330.21.7e-14646.14S-locus lectin protein kinase family protein [more]
AT1G11350.12.8e-14644.24S-domain-1 13 [more]
AT1G11330.12.8e-14646.52S-locus lectin protein kinase family protein [more]
AT1G11300.12.2e-13843.94protein serine/threonine kinases;protein kinases;ATP binding;sugar binding;kinas... [more]
AT1G61390.11.6e-12840.87S-locus lectin protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003609PAN/Apple domainSMARTSM00473ntp_6coord: 351..426
e-value: 6.0E-13
score: 59.0
IPR003609PAN/Apple domainPFAMPF08276PAN_2coord: 351..411
e-value: 1.1E-16
score: 60.6
IPR003609PAN/Apple domainPROSITEPS50948PANcoord: 341..427
score: 9.964023
IPR001480Bulb-type lectin domainSMARTSM00108blect_4coord: 31..149
e-value: 1.3E-39
score: 147.6
IPR001480Bulb-type lectin domainPFAMPF01453B_lectincoord: 73..177
e-value: 5.0E-31
score: 107.3
IPR001480Bulb-type lectin domainPROSITEPS50927BULB_LECTINcoord: 25..146
score: 19.716763
IPR001480Bulb-type lectin domainCDDcd00028B_lectincoord: 31..149
e-value: 6.1636E-36
score: 128.969
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 525..607
e-value: 4.6E-18
score: 65.4
IPR000858S-locus glycoprotein domainPFAMPF00954S_locus_glycopcoord: 209..319
e-value: 7.5E-26
score: 90.8
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 500..598
e-value: 2.8E-37
score: 129.0
NoneNo IPR availablePANTHERPTHR32444FAMILY NOT NAMEDcoord: 16..609
NoneNo IPR availablePANTHERPTHR32444:SF69G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-13coord: 16..609
NoneNo IPR availableCDDcd01098PAN_AP_plantcoord: 337..427
e-value: 1.85182E-24
score: 95.5809
IPR036426Bulb-type lectin domain superfamilyGENE3D2.90.10.10coord: 19..148
e-value: 4.5E-22
score: 80.3
IPR036426Bulb-type lectin domain superfamilySUPERFAMILY51110alpha-D-mannose-specific plant lectinscoord: 68..215
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 523..610
score: 16.075317
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 508..609

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS025001.1MS025001.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0048544 recognition of pollen
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity