MS024974 (gene) Bitter gourd (TR) v1

Overview
NameMS024974
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncalmodulin-binding receptor-like cytoplasmic kinase 2
Locationscaffold254: 1801296 .. 1804415 (+)
RNA-Seq ExpressionMS024974
SyntenyMS024974
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAAGCACAAGTCCATGGAACGGCAGCGTGCGCCGGAACTCCACCTCCGGTGATGAGCGGCGGAACTCTGCTTCCTGTGGGGAGCGGCGGAACTCTGCCTCCGGTAGTGAACGCCGGAACTCTAGCTCTGGTGGTCTGGGAACGCGGATTCAATACCTCTACTCGCCTAGTCCCGATGTGTCCTCCGGTCTGGATTCCCGCTCCCGACGAAATCCGGTGAAAGCCGCTGCACGGTCCGTCGCCGGAGCTTTCATTACTTGCTTTACCCCGCCGGAGGGGGAAACTTCGCCGGGTTATTGTGGAGGATGTAATCTTCCCTCAGGTAATACTTGATTTTGCGGGTTTAAGCGAGGGGAATGAGAAGGTTTTGTGAGTGCGTCTGTTTTCCACTAGGTTTTGGTCTTTTTGAATCATTCTTCGAGAAAAACTGGTAATGGAGACGATTAATCGGCTTTCCGAAGCTGTTAAGCTGCTGTTTAGTGTTATTTCTTTTTCATTAATCTTCGGATCGTGGATGTTCATAAAAAAATTGGCTAATAATTTTCCGAATTGGTTGTTTTCTGCATTATCTCCAATTGAGAACTCCACGTCCTTGCAAGTTGCGAAAACTGTTTCTCCGAGACTCGTTTATCTGTTTCTAATTGACGATCGTTTGTGATCGTGGATTCCTTAATCTTCCAACGTGTTCAGATTTTGTGCGCAAATATTATTCCCATTGTTGGATAACGAGAATAATATAGGAATAAGTGGATTTTTCGCATCACTTGAAAGCCCCTGAAAATGAGAGTTAAGAAAATTCAATTACTATTTTCGGTCTTCGGTAGGCTCGCTTGATTCAAATTGTTATCTCTTTCAAACGGGAAAAGCAGAGCAGTAGAATACTTATTCCAATTGTGATTTTAGGATGGACTTATGTGTTTATTTCTCTGTAATTTCAGATACATCCAGTCATAATGGCAGTAGAAATCAAGGATCTAGACGAGAATCCCACTTCAGTTCAAATCTAGCAGCCCATGATAGAGAGCCTGGATCTTTAAAGATCAGTATAGATGAAATCCGCAAGGCCACGAAGAACTTCTCCACAGCTTCAAAAATTGGTCAAGGGGGTTTTGGGGCTGTCTACAAGGGGAAACTTGATGGAATGCTTGTGGCAATAAAGCGTGCCAAGAAGGTTCAAAATTTTGATTATTAACCAGTTTATGGCTTAAGGCTTTCAAACACAGTTTCTGTTCATTGTTGTTAACTCGAATTCAGTTTTTTCCATAACCCCTTCACTTGCAGAGTGTATATGATAATAATTTGGGCCTGGAGTTTAAAAGCGAGATCCAAACACTGGCACAAGTAGAACATTTAAATCTGGTAAAGTTCTATGGATATCTGGAACATGAAGATGAAAGAATAGTCATTGTAGAATATGTGCCCAATGGCACTCTCAGAGAGCACTTGGAGTGTGAGTTACTTTTTTCTTATCATTATCCTCTCTTTGAACATTCCTTTTACTCTATCTGAATCATGAAACTTGATGGCAGGTATCCATGGAAATATTCTTGACCTTGCTACAAGGCTTGATATTGCAACTGATGTGGCTCATGCAATAACATATCTTCATATGTATACAGGTTTGGATTTCTGATTCAATGCCCAACTCATCTTTAACTTCATACAACTTAGCTTCATTGAACTCTGGGTGAGATTGGGATGGCTTTTGTGAGGGTGAAGAATTGATTTTCTGGGCTAGAAGAACTTGAAAAATTCTTAAAACACTAAAATACTTAAAAGAGTTCTTTTAGGGTAAACACTTAATCAGTGAAAATCACTTCAATCATCCCAAACTAAATCTTAATGTATCCTCCTTGAATTCTACGGGTCATTGTAACTGTTGACTTGTTAAATCGTACATCTTCTGGTTCTTGTTGGCCTGACTAAATTCTTGTGTGTGTAGACCGTCCCATCATTCATAGAGACATAAAGTCTTCCAACATTCTATTGAGTGAGAATTTTAGAGCCAAGGTTGCGGATTTTGGCTTTGCAAGACTTGCAGCTGACGGTGATGCGACCCATGTGTCCACCCAAGTCAAAGGAACTGCTGGCTACTTGGACCCAGAATATCTCAAGACTTATCAACTGACAGAGAAGAGTGACATCTATTCATTTGGTGTTCTATTGGTTGAATTGGTCACGGGCAGGCGCCCCATAGAACCAAAGCGAGAACTTGAACAGCGGGTCACTCCTAAATGGGTTCGACTTTTTCTCTCATCCCTTCATTACTATTATAAGATTTTATATCATAACTGTTGAGGGATACAAGTACCATCTCATGTTGTCTTAAATAAGGCACAACATATGTATTGGTATAAAGTCTTTCAAGTCATATAGGTTCGAGCCCAAATCATATTATATAAATATATAGAAAAACAAGAAAACGAGAGCCTCCAATAAGTACAAAATTAGATAAAAATATCAAGAAACTCGGTGTCATTGTAGTTTGTTCTTCGCACACTGCCCAGTATCAATATTTATGAGATTTCATCTCAAAACCAATTTGAAATAAGAGTAGTCCATATCTATTATAAACCCTTATTAGATCCCTTATATTTTCAACATGGGATCCTTAACATACCCCTCAAAATCGTGTCTTATAACTCATCGATCTTGGTCGGATCACATTTTTTTGGACTGGATGTTCGTTTGAGCATTTTGGACTCTACAACATATCAATATTCATGGAGTTCCATCTCAAAACCAATTGATAATGAGATGAGTAGCCCATGTCTATTATAAACACTTGTTAGGTATCTTATCTTTTCAATGTAGGAAACTTAACACTCTGTGACGATGTTAATGCAGGCCATGAAGAAGTTTGCTGAAGGAGAAGCCAGTACAATTCTGGACCCAAATCTGGAGCAAACAGTTGCTAATAATTTAGCAGTGGAAAAGATTCTAGAGCTGGCCCTACAATGCTTGGCCCCCCGGAGGCAAAGTAGGCCTAGCATGAGGAGGTGTGCAGAGATACTATGGAGCATACGCAAGGATCACAGAGAGCTATCAGCTCCAGATTTGCGTTCATTCTCTTCAAATTCACAGAGGAGCGCCTCGATAAGAGAAAAACGAAACTGA

mRNA sequence

ATGACAAGCACAAGTCCATGGAACGGCAGCGTGCGCCGGAACTCCACCTCCGGTGATGAGCGGCGGAACTCTGCTTCCTGTGGGGAGCGGCGGAACTCTGCCTCCGGTAGTGAACGCCGGAACTCTAGCTCTGGTGGTCTGGGAACGCGGATTCAATACCTCTACTCGCCTAGTCCCGATGTGTCCTCCGGTCTGGATTCCCGCTCCCGACGAAATCCGGTGAAAGCCGCTGCACGGTCCGTCGCCGGAGCTTTCATTACTTGCTTTACCCCGCCGGAGGGGGAAACTTCGCCGGGTTATTGTGGAGGATGTAATCTTCCCTCAGATACATCCAGTCATAATGGCAGTAGAAATCAAGGATCTAGACGAGAATCCCACTTCAGTTCAAATCTAGCAGCCCATGATAGAGAGCCTGGATCTTTAAAGATCAGTATAGATGAAATCCGCAAGGCCACGAAGAACTTCTCCACAGCTTCAAAAATTGGTCAAGGGGGTTTTGGGGCTGTCTACAAGGGGAAACTTGATGGAATGCTTGTGGCAATAAAGCGTGCCAAGAAGAGTGTATATGATAATAATTTGGGCCTGGAGTTTAAAAGCGAGATCCAAACACTGGCACAAGTAGAACATTTAAATCTGGTAAAGTTCTATGGATATCTGGAACATGAAGATGAAAGAATAGTCATTGTAGAATATGTGCCCAATGGCACTCTCAGAGAGCACTTGGAGTGTATCCATGGAAATATTCTTGACCTTGCTACAAGGCTTGATATTGCAACTGATGTGGCTCATGCAATAACATATCTTCATATGTATACAGACCGTCCCATCATTCATAGAGACATAAAGTCTTCCAACATTCTATTGAGTGAGAATTTTAGAGCCAAGGTTGCGGATTTTGGCTTTGCAAGACTTGCAGCTGACGGTGATGCGACCCATGTGTCCACCCAAGTCAAAGGAACTGCTGGCTACTTGGACCCAGAATATCTCAAGACTTATCAACTGACAGAGAAGAGTGACATCTATTCATTTGGTGTTCTATTGGTTGAATTGGTCACGGGCAGGCGCCCCATAGAACCAAAGCGAGAACTTGAACAGCGGGCCATGAAGAAGTTTGCTGAAGGAGAAGCCAGTACAATTCTGGACCCAAATCTGGAGCAAACAGTTGCTAATAATTTAGCAGTGGAAAAGATTCTAGAGCTGGCCCTACAATGCTTGGCCCCCCGGAGGCAAAGTAGGCCTAGCATGAGGAGGTGTGCAGAGATACTATGGAGCATACGCAAGGATCACAGAGAGCTATCAGCTCCAGATTTGCGTTCATTCTCTTCAAATTCACAGAGGAGCGCCTCGATAAGAGAAAAACGAAACTGA

Coding sequence (CDS)

ATGACAAGCACAAGTCCATGGAACGGCAGCGTGCGCCGGAACTCCACCTCCGGTGATGAGCGGCGGAACTCTGCTTCCTGTGGGGAGCGGCGGAACTCTGCCTCCGGTAGTGAACGCCGGAACTCTAGCTCTGGTGGTCTGGGAACGCGGATTCAATACCTCTACTCGCCTAGTCCCGATGTGTCCTCCGGTCTGGATTCCCGCTCCCGACGAAATCCGGTGAAAGCCGCTGCACGGTCCGTCGCCGGAGCTTTCATTACTTGCTTTACCCCGCCGGAGGGGGAAACTTCGCCGGGTTATTGTGGAGGATGTAATCTTCCCTCAGATACATCCAGTCATAATGGCAGTAGAAATCAAGGATCTAGACGAGAATCCCACTTCAGTTCAAATCTAGCAGCCCATGATAGAGAGCCTGGATCTTTAAAGATCAGTATAGATGAAATCCGCAAGGCCACGAAGAACTTCTCCACAGCTTCAAAAATTGGTCAAGGGGGTTTTGGGGCTGTCTACAAGGGGAAACTTGATGGAATGCTTGTGGCAATAAAGCGTGCCAAGAAGAGTGTATATGATAATAATTTGGGCCTGGAGTTTAAAAGCGAGATCCAAACACTGGCACAAGTAGAACATTTAAATCTGGTAAAGTTCTATGGATATCTGGAACATGAAGATGAAAGAATAGTCATTGTAGAATATGTGCCCAATGGCACTCTCAGAGAGCACTTGGAGTGTATCCATGGAAATATTCTTGACCTTGCTACAAGGCTTGATATTGCAACTGATGTGGCTCATGCAATAACATATCTTCATATGTATACAGACCGTCCCATCATTCATAGAGACATAAAGTCTTCCAACATTCTATTGAGTGAGAATTTTAGAGCCAAGGTTGCGGATTTTGGCTTTGCAAGACTTGCAGCTGACGGTGATGCGACCCATGTGTCCACCCAAGTCAAAGGAACTGCTGGCTACTTGGACCCAGAATATCTCAAGACTTATCAACTGACAGAGAAGAGTGACATCTATTCATTTGGTGTTCTATTGGTTGAATTGGTCACGGGCAGGCGCCCCATAGAACCAAAGCGAGAACTTGAACAGCGGGCCATGAAGAAGTTTGCTGAAGGAGAAGCCAGTACAATTCTGGACCCAAATCTGGAGCAAACAGTTGCTAATAATTTAGCAGTGGAAAAGATTCTAGAGCTGGCCCTACAATGCTTGGCCCCCCGGAGGCAAAGTAGGCCTAGCATGAGGAGGTGTGCAGAGATACTATGGAGCATACGCAAGGATCACAGAGAGCTATCAGCTCCAGATTTGCGTTCATTCTCTTCAAATTCACAGAGGAGCGCCTCGATAAGAGAAAAACGAAACTGA

Protein sequence

MTSTSPWNGSVRRNSTSGDERRNSASCGERRNSASGSERRNSSSGGLGTRIQYLYSPSPDVSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNLPSDTSSHNGSRNQGSRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGMLVAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELEQRAMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSMRRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREKRN
Homology
BLAST of MS024974 vs. NCBI nr
Match: XP_022147253.1 (calmodulin-binding receptor-like cytoplasmic kinase 2 [Momordica charantia])

HSP 1 Score: 865.5 bits (2235), Expect = 2.0e-247
Identity = 452/460 (98.26%), Postives = 453/460 (98.48%), Query Frame = 0

Query: 1   MTSTSPWNGSVRRNSTSGDERRNSASCGERRNSASGSERRNSSSGGLGTRIQYLYSPSPD 60
           MTSTSPWNGSVRRNSTSGDERRNSAS GERRNSASG ERRNSSSGGLGTRIQYLYSPSPD
Sbjct: 1   MTSTSPWNGSVRRNSTSGDERRNSASGGERRNSASGGERRNSSSGGLGTRIQYLYSPSPD 60

Query: 61  VSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNLPSDTSSHNGSRNQG 120
           VSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNLPSDTSSHNGSRNQG
Sbjct: 61  VSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNLPSDTSSHNGSRNQG 120

Query: 121 SRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGMLVA 180
           SRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGMLVA
Sbjct: 121 SRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGMLVA 180

Query: 181 IKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREH 240
           IKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREH
Sbjct: 181 IKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREH 240

Query: 241 LECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFG 300
           LECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFG
Sbjct: 241 LECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFG 300

Query: 301 FARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPK 360
           FARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLL+ELVTGRRPIEPK
Sbjct: 301 FARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLIELVTGRRPIEPK 360

Query: 361 RELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSM 420
           RELEQR     AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSM
Sbjct: 361 RELEQRVTPKWAMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSM 420

Query: 421 RRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREKRN 456
           RRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREKRN
Sbjct: 421 RRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREKRN 460

BLAST of MS024974 vs. NCBI nr
Match: XP_023526304.1 (calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 733.8 bits (1893), Expect = 9.0e-208
Identity = 394/468 (84.19%), Postives = 419/468 (89.53%), Query Frame = 0

Query: 1   MTSTSPWNGSVRRNSTSGDERRNSASCGERRNSASGSERRNSS-------SGGLGTRIQY 60
           MTS+SPW+G  RRNS+S  ERR S+S GERR S+S  ERRNSS       S   G+R  Y
Sbjct: 1   MTSSSPWHGGERRNSSSSGERRYSSSSGERRYSSSSGERRNSSSVSERRNSCSRGSRSPY 60

Query: 61  L-YSPSPDVSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNL--PSDT 120
           L Y PSPDVS+G++SRSR NPVKAAARSVAGAFITCFTPPE ETSPG+  G      SD 
Sbjct: 61  LSYLPSPDVSAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGREFRHSSDA 120

Query: 121 SSHNGSRNQGSRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVY 180
           SSHN  RNQGSRRE+HFSSNLAAHDREPGSL+ISI+EIRKATKNFS +SKIGQGGFGAVY
Sbjct: 121 SSHNSKRNQGSRRETHFSSNLAAHDREPGSLRISIEEIRKATKNFSASSKIGQGGFGAVY 180

Query: 181 KGKLDGMLVAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVE 240
           KGKLDG+LVAIKRAKKSVYDNNLG+EF+SEIQTLAQVEHLNLVKFYGYLEHEDERIVIVE
Sbjct: 181 KGKLDGVLVAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVE 240

Query: 241 YVPNGTLREHLECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSE 300
           YVPNGTLREHLECIHG  LDLATRL IATDVAHAITYLHMYTDRPIIHRDIKSSNILL+E
Sbjct: 241 YVPNGTLREHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTE 300

Query: 301 NFRAKVADFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVEL 360
           N+RAKVADFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVEL
Sbjct: 301 NYRAKVADFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVEL 360

Query: 361 VTGRRPIEPKRELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCL 420
           VTGRRPIEPKREL+QR     AMKKF EGEASTILDPNLEQT AN+ AVEKILELALQCL
Sbjct: 361 VTGRRPIEPKRELDQRITPKWAMKKFCEGEASTILDPNLEQTAANHSAVEKILELALQCL 420

Query: 421 APRRQSRPSMRRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREK 454
           APRR SRPSMRRCAEILWSIRKDHR+L+APDLRSFS+NSQRSAS+REK
Sbjct: 421 APRRHSRPSMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 468

BLAST of MS024974 vs. NCBI nr
Match: KAG6582621.1 (Calmodulin-binding receptor-like cytoplasmic kinase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 733.0 bits (1891), Expect = 1.5e-207
Identity = 392/461 (85.03%), Postives = 418/461 (90.67%), Query Frame = 0

Query: 1   MTSTSPWNGSVRRNSTSGDERRNSASCGERRNSASGSERRNSSSGGLGTRIQYL-YSPSP 60
           MTS+SPW+G  RR S+S  ERR S+S GERRNS+S SERRNSSS    +R  YL Y PSP
Sbjct: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSS--RCSRSPYLSYLPSP 60

Query: 61  DVSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNL--PSDTSSHNGSR 120
           DVS+G++SRSR NPVKAAARSVAGAFITCFTPPE ETSPG+  G      SD SSHN  R
Sbjct: 61  DVSAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKR 120

Query: 121 NQGSRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGM 180
           NQGSRRE+HFSSNLAAHDREPGSL+ISI+EIRKATKNFS +SKIGQGGFGAVYKGKLDG+
Sbjct: 121 NQGSRRETHFSSNLAAHDREPGSLRISIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGV 180

Query: 181 LVAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL 240
           LVAIKRAKKSVYDNNLG+EF+SEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL
Sbjct: 181 LVAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL 240

Query: 241 REHLECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVA 300
           REHLECIHG  LDLATRL IATDVAHAITYLHMYTDRPIIHRDIKSSNILL+EN+RAKVA
Sbjct: 241 REHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVA 300

Query: 301 DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI 360
           DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI
Sbjct: 301 DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI 360

Query: 361 EPKRELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSR 420
           EPKREL+QR     AMKKF EGEAST+LDPNLEQT AN+ A EKILELALQCLAPRR SR
Sbjct: 361 EPKRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSR 420

Query: 421 PSMRRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREK 454
           PSMRRCAEILWSIRKDHR+L+APDLRSFS+NSQRSAS+REK
Sbjct: 421 PSMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 459

BLAST of MS024974 vs. NCBI nr
Match: XP_022924490.1 (calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita moschata])

HSP 1 Score: 732.3 bits (1889), Expect = 2.6e-207
Identity = 392/461 (85.03%), Postives = 418/461 (90.67%), Query Frame = 0

Query: 1   MTSTSPWNGSVRRNSTSGDERRNSASCGERRNSASGSERRNSSSGGLGTRIQYL-YSPSP 60
           MTS+SPW+G  RR S+S  ERR S+S GERRNS+S SERRNSSS   G+   YL Y PSP
Sbjct: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSGS--PYLSYLPSP 60

Query: 61  DVSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNL--PSDTSSHNGSR 120
           DVS+G++SRSR NPVKAAARSVAGAFITCFTPPE ETSPG+  G      SD SSHN  R
Sbjct: 61  DVSAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKR 120

Query: 121 NQGSRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGM 180
           NQGSRRE+HFSSNLAAHDREPGSL+ISI+EIRKATKNFS +SKIGQGGFGAVYKGKLDG+
Sbjct: 121 NQGSRRETHFSSNLAAHDREPGSLRISIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGV 180

Query: 181 LVAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL 240
           LVAIKRAKKSVYDNNLG+EF+SEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL
Sbjct: 181 LVAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL 240

Query: 241 REHLECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVA 300
           REHLECIHG  LDLATRL IATDVAHAITYLHMYTDRPIIHRDIKSSNILL+EN+RAKVA
Sbjct: 241 REHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVA 300

Query: 301 DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI 360
           DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI
Sbjct: 301 DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI 360

Query: 361 EPKRELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSR 420
           EPKREL+QR     AMKKF EGEAST+LDPNLEQT AN+ A EKILELALQCLAPRR SR
Sbjct: 361 EPKRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSR 420

Query: 421 PSMRRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREK 454
           PSMRRCAEILWSIRKDHR+L+APDLRSFS+NSQRSAS+REK
Sbjct: 421 PSMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 459

BLAST of MS024974 vs. NCBI nr
Match: KAG7019016.1 (Calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 731.9 bits (1888), Expect = 3.4e-207
Identity = 391/461 (84.82%), Postives = 418/461 (90.67%), Query Frame = 0

Query: 1   MTSTSPWNGSVRRNSTSGDERRNSASCGERRNSASGSERRNSSSGGLGTRIQYL-YSPSP 60
           MTS+SPW+G  RR S+S  ERR S+S GERRNS+S SERRNSSS    +R  YL Y PSP
Sbjct: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSS--RCSRSPYLSYLPSP 60

Query: 61  DVSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNL--PSDTSSHNGSR 120
           DVS+G++SRSR NPVKAAARSVAGAFITCFTPPE ETSPG+  G      SD SSHN  R
Sbjct: 61  DVSAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKR 120

Query: 121 NQGSRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGM 180
           NQGSRRE+HFSSNLAAHDREPGSL+IS++EIRKATKNFS +SKIGQGGFGAVYKGKLDG+
Sbjct: 121 NQGSRRETHFSSNLAAHDREPGSLRISMEEIRKATKNFSASSKIGQGGFGAVYKGKLDGV 180

Query: 181 LVAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL 240
           LVAIKRAKKSVYDNNLG+EF+SEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL
Sbjct: 181 LVAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL 240

Query: 241 REHLECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVA 300
           REHLECIHG  LDLATRL IATDVAHAITYLHMYTDRPIIHRDIKSSNILL+EN+RAKVA
Sbjct: 241 REHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVA 300

Query: 301 DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI 360
           DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI
Sbjct: 301 DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI 360

Query: 361 EPKRELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSR 420
           EPKREL+QR     AMKKF EGEAST+LDPNLEQT AN+ A EKILELALQCLAPRR SR
Sbjct: 361 EPKRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSR 420

Query: 421 PSMRRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREK 454
           PSMRRCAEILWSIRKDHR+L+APDLRSFS+NSQRSAS+REK
Sbjct: 421 PSMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 459

BLAST of MS024974 vs. ExPASy Swiss-Prot
Match: Q8VZJ9 (Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Arabidopsis thaliana OX=3702 GN=CRCK2 PE=2 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 4.3e-120
Identity = 235/378 (62.17%), Postives = 282/378 (74.60%), Query Frame = 0

Query: 67  SRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNLPSDTS--SHNGSRNQGSRRE 126
           +R+  +   + AR ++G FI CFTPP+   S  Y       SD +  S   S    S + 
Sbjct: 36  ARTSTDTTTSIARRISGIFINCFTPPD-SVSSNYIDNSKSSSDNNIRSRRSSTGSSSVQR 95

Query: 127 SHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKL-DGMLVAIKR 186
           S+ ++N   H R       + DEI  ATKNFS + +IGQGGFG VYK KL DG   A+KR
Sbjct: 96  SYGNANETEHTR------FTFDEIYDATKNFSPSFRIGQGGFGTVYKVKLRDGKTFAVKR 155

Query: 187 AKKSVYDNNLG--LEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHL 246
           AKKS++D+  G   EF SEIQTLAQV HL+LVK+YG++ H DE+I++VEYV NGTLR+HL
Sbjct: 156 AKKSMHDDRQGADAEFMSEIQTLAQVTHLSLVKYYGFVVHNDEKILVVEYVANGTLRDHL 215

Query: 247 ECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFGF 306
           +C  G  LD+ATRLDIATDVAHAITYLHMYT  PIIHRDIKSSNILL+EN+RAKVADFGF
Sbjct: 216 DCKEGKTLDMATRLDIATDVAHAITYLHMYTQPPIIHRDIKSSNILLTENYRAKVADFGF 275

Query: 307 ARLAADGD--ATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 366
           ARLA D D  ATHVSTQVKGTAGYLDPEYL TYQLTEKSD+YSFGVLLVEL+TGRRPIE 
Sbjct: 276 ARLAPDTDSGATHVSTQVKGTAGYLDPEYLTTYQLTEKSDVYSFGVLLVELLTGRRPIEL 335

Query: 367 KRELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPS 426
            R  ++R     A+KKF  G+  ++LDP LEQ  ANNLA+EK+LE+A QCLAP R+SRPS
Sbjct: 336 SRGQKERITIRWAIKKFTSGDTISVLDPKLEQNSANNLALEKVLEMAFQCLAPHRRSRPS 395

Query: 427 MRRCAEILWSIRKDHREL 433
           M++C+EILW IRKD+REL
Sbjct: 396 MKKCSEILWGIRKDYREL 406

BLAST of MS024974 vs. ExPASy Swiss-Prot
Match: Q9FIL7 (Calmodulin-binding receptor-like cytoplasmic kinase 1 OS=Arabidopsis thaliana OX=3702 GN=CRCK1 PE=1 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 4.1e-94
Identity = 185/340 (54.41%), Postives = 251/340 (73.82%), Query Frame = 0

Query: 117 RNQGSRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKL-D 176
           R  GSR +S    + ++     G +  S  E+++AT NFS+  +IG+GGFG V+KGKL D
Sbjct: 109 RFSGSRFQSPGKDSSSSKSWHQGPVIFSFGELQRATANFSSVHQIGEGGFGTVFKGKLDD 168

Query: 177 GMLVAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNG 236
           G +VAIKRA+K+ Y  +  LEFK+EI TL+++EH+NLVK YG+LEH DE++++VEYV NG
Sbjct: 169 GTIVAIKRARKNNYGKSWLLEFKNEIYTLSKIEHMNLVKLYGFLEHGDEKVIVVEYVANG 228

Query: 237 TLREHLECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAK 296
            LREHL+ + GN L++A RL+IA DVAHA+TYLH YTD PIIHRDIK+SNIL++   RAK
Sbjct: 229 NLREHLDGLRGNRLEMAERLEIAIDVAHALTYLHTYTDSPIIHRDIKASNILITNKLRAK 288

Query: 297 VADFGFARLAA-DGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGR 356
           VADFGFARL + D  ATH+STQVKG+AGY+DP+YL+T+QLT+KSD+YSFGVLLVE++TGR
Sbjct: 289 VADFGFARLVSEDLGATHISTQVKGSAGYVDPDYLRTFQLTDKSDVYSFGVLLVEILTGR 348

Query: 357 RPIEPKRELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRR 416
           RPIE KR  + R     A+++  + EA  I+DP L++  A     EK+L LA +C+ P R
Sbjct: 349 RPIELKRPRKDRLTVKWALRRLKDDEAVLIMDPFLKRNRAAIEVAEKMLRLASECVTPTR 408

Query: 417 QSRPSMRRCAEILWSIRKDHRELSAPDLRSFSSNSQRSAS 450
            +RP+M+  AE LW+IR   RE+    + S +SNS  S++
Sbjct: 409 ATRPAMKGIAEKLWAIR---REMKETMICSSASNSSCSST 445

BLAST of MS024974 vs. ExPASy Swiss-Prot
Match: Q9ASQ5 (Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Arabidopsis thaliana OX=3702 GN=CRCK3 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 3.0e-81
Identity = 157/298 (52.68%), Postives = 208/298 (69.80%), Query Frame = 0

Query: 139 GSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKL-DGMLVAIKRAKKSVYDNNLGLEF 198
           G L +++ +I  AT NF+ + +IG+GGFG V+KG L DG +VAIKRAKK  ++ NL  EF
Sbjct: 209 GPLNLTMSQINTATGNFADSHQIGEGGFGVVFKGVLDDGQVVAIKRAKKEHFE-NLRTEF 268

Query: 199 KSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGNILDLATRLDI 258
           KSE+  L+++ H NLVK  GY++  DER++I EYV NGTLR+HL+   G  L+   RL+I
Sbjct: 269 KSEVDLLSKIGHRNLVKLLGYVDKGDERLIITEYVRNGTLRDHLDGARGTKLNFNQRLEI 328

Query: 259 ATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFGFARLA-ADGDATHVSTQ 318
             DV H +TYLH Y +R IIHRDIKSSNILL+++ RAKVADFGFAR    D + TH+ TQ
Sbjct: 329 VIDVCHGLTYLHSYAERQIIHRDIKSSNILLTDSMRAKVADFGFARGGPTDSNQTHILTQ 388

Query: 319 VKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELEQR-----AMKKF 378
           VKGT GYLDPEY+KTY LT KSD+YSFG+LLVE++TGRRP+E KR  ++R     A  K+
Sbjct: 389 VKGTVGYLDPEYMKTYHLTAKSDVYSFGILLVEILTGRRPVEAKRLPDERITVRWAFDKY 448

Query: 379 AEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSMRRCAEILWSIRKDH 430
            EG    ++DPN  + V   + + K+  LA QC AP ++ RP M    + LW+IR  +
Sbjct: 449 NEGRVFELVDPNARERVDEKI-LRKMFSLAFQCAAPTKKERPDMEAVGKQLWAIRSSY 504

BLAST of MS024974 vs. ExPASy Swiss-Prot
Match: Q9LT96 (Probable leucine-rich repeat receptor-like protein kinase At5g49770 OS=Arabidopsis thaliana OX=3702 GN=At5g49770 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.4e-62
Identity = 129/292 (44.18%), Postives = 184/292 (63.01%), Query Frame = 0

Query: 139 GSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKL-DGMLVAIKRAKKSVYDNNLGLEF 198
           G+   + +E+ K T NFS A+ +G GG+G VYKG L +G ++AIKRA++         EF
Sbjct: 618 GTKAFTFEELSKCTNNFSDANDVGGGGYGQVYKGTLPNGQVIAIKRAQQGSMQG--AFEF 677

Query: 199 KSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGNILDLATRLDI 258
           K+EI+ L++V H N+VK  G+   + E++++ EY+PNG+LR+ L   +G  LD   RL I
Sbjct: 678 KTEIELLSRVHHKNVVKLLGFCFDQKEQMLVYEYIPNGSLRDGLSGKNGVKLDWTRRLKI 737

Query: 259 ATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFGFARLAADGDATHVSTQV 318
           A      + YLH   D PIIHRD+KS+NILL E+  AKVADFG ++L  D +  HV+TQV
Sbjct: 738 ALGSGKGLAYLHELADPPIIHRDVKSNNILLDEHLTAKVADFGLSKLVGDPEKAHVTTQV 797

Query: 319 KGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELEQRAMKKFAEG--- 378
           KGT GYLDPEY  T QLTEKSD+Y FGV+++EL+TG+ PI+    + +   KK  +    
Sbjct: 798 KGTMGYLDPEYYMTNQLTEKSDVYGFGVVMLELLTGKSPIDRGSYVVKEVKKKMDKSRNL 857

Query: 379 -EASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSMRRCAEILWSI 426
            +   +LD  + Q   N    EK +++ALQC+ P   +RP+M    + L SI
Sbjct: 858 YDLQELLDTTIIQNSGNLKGFEKYVDVALQCVEPEGVNRPTMSEVVQELESI 907

BLAST of MS024974 vs. ExPASy Swiss-Prot
Match: Q8GZ99 (Leucine-rich repeat receptor protein kinase HPCA1 OS=Arabidopsis thaliana OX=3702 GN=HPCA1 PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 5.4e-62
Identity = 137/314 (43.63%), Postives = 197/314 (62.74%), Query Frame = 0

Query: 139 GSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKL-DGMLVAIKRAKKSVYDNNLGLEF 198
           G+   + +E++K T NFS A+ +G GG+G VY+G L +G L+AIKRA++       GLEF
Sbjct: 615 GAKAFTFEELKKCTDNFSEANDVGGGGYGKVYRGILPNGQLIAIKRAQQGSLQG--GLEF 674

Query: 199 KSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGNILDLATRLDI 258
           K+EI+ L++V H N+V+  G+    +E++++ EY+ NG+L++ L    G  LD   RL I
Sbjct: 675 KTEIELLSRVHHKNVVRLLGFCFDRNEQMLVYEYISNGSLKDSLSGKSGIRLDWTRRLKI 734

Query: 259 ATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFGFARLAADGDATHVSTQV 318
           A      + YLH   D PIIHRDIKS+NILL EN  AKVADFG ++L  D + THV+TQV
Sbjct: 735 ALGSGKGLAYLHELADPPIIHRDIKSNNILLDENLTAKVADFGLSKLVGDPEKTHVTTQV 794

Query: 319 KGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE----PKRELEQRAMKKFAE 378
           KGT GYLDPEY  T QLTEKSD+Y FGV+L+EL+TGR PIE      RE++ +  K  + 
Sbjct: 795 KGTMGYLDPEYYMTNQLTEKSDVYGFGVVLLELLTGRSPIERGKYVVREVKTKMNKSRSL 854

Query: 379 GEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSMRRCAEILWSIRKDHRELS 438
            +   +LD  +  +  N    EK ++LAL+C+     +RPSM    + + +I     +L+
Sbjct: 855 YDLQELLDTTIIASSGNLKGFEKYVDLALRCVEEEGVNRPSMGEVVKEIENI----MQLA 914

Query: 439 APDLRSFSSNSQRS 448
             +  S S+ S R+
Sbjct: 915 GLNPNSDSATSSRT 922

BLAST of MS024974 vs. ExPASy TrEMBL
Match: A0A6J1D0T1 (calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Momordica charantia OX=3673 GN=LOC111016250 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 9.6e-248
Identity = 452/460 (98.26%), Postives = 453/460 (98.48%), Query Frame = 0

Query: 1   MTSTSPWNGSVRRNSTSGDERRNSASCGERRNSASGSERRNSSSGGLGTRIQYLYSPSPD 60
           MTSTSPWNGSVRRNSTSGDERRNSAS GERRNSASG ERRNSSSGGLGTRIQYLYSPSPD
Sbjct: 1   MTSTSPWNGSVRRNSTSGDERRNSASGGERRNSASGGERRNSSSGGLGTRIQYLYSPSPD 60

Query: 61  VSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNLPSDTSSHNGSRNQG 120
           VSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNLPSDTSSHNGSRNQG
Sbjct: 61  VSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNLPSDTSSHNGSRNQG 120

Query: 121 SRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGMLVA 180
           SRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGMLVA
Sbjct: 121 SRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGMLVA 180

Query: 181 IKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREH 240
           IKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREH
Sbjct: 181 IKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREH 240

Query: 241 LECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFG 300
           LECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFG
Sbjct: 241 LECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFG 300

Query: 301 FARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPK 360
           FARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLL+ELVTGRRPIEPK
Sbjct: 301 FARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLIELVTGRRPIEPK 360

Query: 361 RELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSM 420
           RELEQR     AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSM
Sbjct: 361 RELEQRVTPKWAMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSM 420

Query: 421 RRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREKRN 456
           RRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREKRN
Sbjct: 421 RRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREKRN 460

BLAST of MS024974 vs. ExPASy TrEMBL
Match: A0A6J1EF63 (calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucurbita moschata OX=3662 GN=LOC111431968 PE=3 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 1.3e-207
Identity = 392/461 (85.03%), Postives = 418/461 (90.67%), Query Frame = 0

Query: 1   MTSTSPWNGSVRRNSTSGDERRNSASCGERRNSASGSERRNSSSGGLGTRIQYL-YSPSP 60
           MTS+SPW+G  RR S+S  ERR S+S GERRNS+S SERRNSSS   G+   YL Y PSP
Sbjct: 1   MTSSSPWHGGERRYSSSSGERRYSSSSGERRNSSSVSERRNSSSRCSGS--PYLSYLPSP 60

Query: 61  DVSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNL--PSDTSSHNGSR 120
           DVS+G++SRSR NPVKAAARSVAGAFITCFTPPE ETSPG+  G      SD SSHN  R
Sbjct: 61  DVSAGMESRSRLNPVKAAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSSDASSHNSKR 120

Query: 121 NQGSRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGM 180
           NQGSRRE+HFSSNLAAHDREPGSL+ISI+EIRKATKNFS +SKIGQGGFGAVYKGKLDG+
Sbjct: 121 NQGSRRETHFSSNLAAHDREPGSLRISIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGV 180

Query: 181 LVAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL 240
           LVAIKRAKKSVYDNNLG+EF+SEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL
Sbjct: 181 LVAIKRAKKSVYDNNLGVEFQSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL 240

Query: 241 REHLECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVA 300
           REHLECIHG  LDLATRL IATDVAHAITYLHMYTDRPIIHRDIKSSNILL+EN+RAKVA
Sbjct: 241 REHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVA 300

Query: 301 DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI 360
           DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI
Sbjct: 301 DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI 360

Query: 361 EPKRELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSR 420
           EPKREL+QR     AMKKF EGEAST+LDPNLEQT AN+ A EKILELALQCLAPRR SR
Sbjct: 361 EPKRELDQRITPKWAMKKFCEGEASTLLDPNLEQTTANHSAAEKILELALQCLAPRRHSR 420

Query: 421 PSMRRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREK 454
           PSMRRCAEILWSIRKDHR+L+APDLRSFS+NSQRSAS+REK
Sbjct: 421 PSMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 459

BLAST of MS024974 vs. ExPASy TrEMBL
Match: A0A6J1IRI1 (calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucurbita maxima OX=3661 GN=LOC111479865 PE=3 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 5.0e-204
Identity = 388/461 (84.16%), Postives = 412/461 (89.37%), Query Frame = 0

Query: 1   MTSTSPWNGSVRRNSTSGDERRNSASCGERRNSASGSERRNSSSGGLGTRIQYL-YSPSP 60
           MTS+SPW+G          ERR S+S GERRNS+S SERRN SS    +R  YL Y PSP
Sbjct: 1   MTSSSPWHGG---------ERRYSSSSGERRNSSSVSERRNPSS--RCSRSPYLSYLPSP 60

Query: 61  DVSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNL--PSDTSSHNGSR 120
           DVS+G++SRSR NPVK+AARSVAGAFITCFTPPE ETSPG+  G       D SSHN  R
Sbjct: 61  DVSAGMESRSRLNPVKSAARSVAGAFITCFTPPEKETSPGFGIGSEFRHSLDASSHNSKR 120

Query: 121 NQGSRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGM 180
           NQGSRRE+HFSSNLAAHDREPGSL+ISI+EIRKATKNFS +SKIGQGGFGAVYKGKLDG+
Sbjct: 121 NQGSRRETHFSSNLAAHDREPGSLRISIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGV 180

Query: 181 LVAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL 240
           LVAIKRAKKSVYDNNLG+EFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL
Sbjct: 181 LVAIKRAKKSVYDNNLGVEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTL 240

Query: 241 REHLECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVA 300
           REHLECIHG  LDLATRL IATDVAHAITYLHMYTDRPIIHRDIKSSNILL+EN+RAKVA
Sbjct: 241 REHLECIHGTTLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVA 300

Query: 301 DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI 360
           DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI
Sbjct: 301 DFGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPI 360

Query: 361 EPKRELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSR 420
           EPKREL+QR     AMKKF EGEASTILDPNLEQT AN+ AVEKILELALQCLAPRR SR
Sbjct: 361 EPKRELDQRITPKWAMKKFCEGEASTILDPNLEQTAANHSAVEKILELALQCLAPRRHSR 420

Query: 421 PSMRRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREK 454
           PSMRRCAEILWSIRKDHR+L+APDLRSFS+NSQRSAS+REK
Sbjct: 421 PSMRRCAEILWSIRKDHRDLTAPDLRSFSTNSQRSASVREK 450

BLAST of MS024974 vs. ExPASy TrEMBL
Match: A0A5A7U166 (Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001790 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.8e-202
Identity = 384/460 (83.48%), Postives = 407/460 (88.48%), Query Frame = 0

Query: 1   MTSTSPWNGSVRRNSTSGDERRNSASCGERRNSASGSERRNSSSGGLGTRIQYLYSPSPD 60
           MTS SPW+G          ERRNS S GERRNS+S SERRNSSS G  T + + YSPSPD
Sbjct: 1   MTSPSPWHGG---------ERRNSTSSGERRNSSSVSERRNSSSRGSRTPVYFPYSPSPD 60

Query: 61  VSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGET--SPGYCGGCNLPSDTSSHNGSRN 120
           VS+G++SRSR NPVKAAARS AGAF +CFTPPE ET  S G+       SD SSHNG RN
Sbjct: 61  VSTGMESRSRHNPVKAAARSFAGAFTSCFTPPEKETPRSLGFGSELRNSSDASSHNGKRN 120

Query: 121 QGSRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGML 180
           QGSRRE   SSNL AHDREPGSL+I+I+EIRKATKNFS +SKIGQGGFGAVYKGKLDG+L
Sbjct: 121 QGSRREPRSSSNL-AHDREPGSLRINIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVL 180

Query: 181 VAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLR 240
           VAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEH+DERIVIVEYVPNGTLR
Sbjct: 181 VAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHQDERIVIVEYVPNGTLR 240

Query: 241 EHLECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVAD 300
           EHLECIHG +LDLATRL IATDVAHAITYLHMYTDRPIIHRDIKSSNILL+EN+RAKVAD
Sbjct: 241 EHLECIHGTVLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVAD 300

Query: 301 FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE 360
           FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE
Sbjct: 301 FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE 360

Query: 361 PKRELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRP 420
           PKRELEQR     AMKKF+EGEAS ILDPNLEQT AN+LAVEKILELALQCLAPRR SRP
Sbjct: 361 PKRELEQRITPKWAMKKFSEGEASAILDPNLEQTEANHLAVEKILELALQCLAPRRHSRP 420

Query: 421 SMRRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREK 454
           SMRRCAEILW IRKDHR+LSAPD RSFS+NSQRSAS+REK
Sbjct: 421 SMRRCAEILWRIRKDHRDLSAPDFRSFSTNSQRSASVREK 450

BLAST of MS024974 vs. ExPASy TrEMBL
Match: A0A1S3AVY6 (calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucumis melo OX=3656 GN=LOC103483260 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.8e-202
Identity = 384/460 (83.48%), Postives = 407/460 (88.48%), Query Frame = 0

Query: 1   MTSTSPWNGSVRRNSTSGDERRNSASCGERRNSASGSERRNSSSGGLGTRIQYLYSPSPD 60
           MTS SPW+G          ERRNS S GERRNS+S SERRNSSS G  T + + YSPSPD
Sbjct: 1   MTSPSPWHGG---------ERRNSTSSGERRNSSSVSERRNSSSRGSRTPVYFPYSPSPD 60

Query: 61  VSSGLDSRSRRNPVKAAARSVAGAFITCFTPPEGET--SPGYCGGCNLPSDTSSHNGSRN 120
           VS+G++SRSR NPVKAAARS AGAF +CFTPPE ET  S G+       SD SSHNG RN
Sbjct: 61  VSTGMESRSRHNPVKAAARSFAGAFTSCFTPPEKETPRSLGFGSELRNSSDASSHNGKRN 120

Query: 121 QGSRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKLDGML 180
           QGSRRE   SSNL AHDREPGSL+I+I+EIRKATKNFS +SKIGQGGFGAVYKGKLDG+L
Sbjct: 121 QGSRREPRSSSNL-AHDREPGSLRINIEEIRKATKNFSASSKIGQGGFGAVYKGKLDGVL 180

Query: 181 VAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLR 240
           VAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEH+DERIVIVEYVPNGTLR
Sbjct: 181 VAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHQDERIVIVEYVPNGTLR 240

Query: 241 EHLECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVAD 300
           EHLECIHG +LDLATRL IATDVAHAITYLHMYTDRPIIHRDIKSSNILL+EN+RAKVAD
Sbjct: 241 EHLECIHGTVLDLATRLAIATDVAHAITYLHMYTDRPIIHRDIKSSNILLTENYRAKVAD 300

Query: 301 FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE 360
           FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE
Sbjct: 301 FGFARLAADGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE 360

Query: 361 PKRELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRP 420
           PKRELEQR     AMKKF+EGEAS ILDPNLEQT AN+LAVEKILELALQCLAPRR SRP
Sbjct: 361 PKRELEQRITPKWAMKKFSEGEASAILDPNLEQTEANHLAVEKILELALQCLAPRRHSRP 420

Query: 421 SMRRCAEILWSIRKDHRELSAPDLRSFSSNSQRSASIREK 454
           SMRRCAEILW IRKDHR+LSAPD RSFS+NSQRSAS+REK
Sbjct: 421 SMRRCAEILWRIRKDHRDLSAPDFRSFSTNSQRSASVREK 450

BLAST of MS024974 vs. TAIR 10
Match: AT4G00330.1 (calmodulin-binding receptor-like cytoplasmic kinase 2 )

HSP 1 Score: 433.0 bits (1112), Expect = 3.1e-121
Identity = 235/378 (62.17%), Postives = 282/378 (74.60%), Query Frame = 0

Query: 67  SRSRRNPVKAAARSVAGAFITCFTPPEGETSPGYCGGCNLPSDTS--SHNGSRNQGSRRE 126
           +R+  +   + AR ++G FI CFTPP+   S  Y       SD +  S   S    S + 
Sbjct: 36  ARTSTDTTTSIARRISGIFINCFTPPD-SVSSNYIDNSKSSSDNNIRSRRSSTGSSSVQR 95

Query: 127 SHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKL-DGMLVAIKR 186
           S+ ++N   H R       + DEI  ATKNFS + +IGQGGFG VYK KL DG   A+KR
Sbjct: 96  SYGNANETEHTR------FTFDEIYDATKNFSPSFRIGQGGFGTVYKVKLRDGKTFAVKR 155

Query: 187 AKKSVYDNNLG--LEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHL 246
           AKKS++D+  G   EF SEIQTLAQV HL+LVK+YG++ H DE+I++VEYV NGTLR+HL
Sbjct: 156 AKKSMHDDRQGADAEFMSEIQTLAQVTHLSLVKYYGFVVHNDEKILVVEYVANGTLRDHL 215

Query: 247 ECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFGF 306
           +C  G  LD+ATRLDIATDVAHAITYLHMYT  PIIHRDIKSSNILL+EN+RAKVADFGF
Sbjct: 216 DCKEGKTLDMATRLDIATDVAHAITYLHMYTQPPIIHRDIKSSNILLTENYRAKVADFGF 275

Query: 307 ARLAADGD--ATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEP 366
           ARLA D D  ATHVSTQVKGTAGYLDPEYL TYQLTEKSD+YSFGVLLVEL+TGRRPIE 
Sbjct: 276 ARLAPDTDSGATHVSTQVKGTAGYLDPEYLTTYQLTEKSDVYSFGVLLVELLTGRRPIEL 335

Query: 367 KRELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPS 426
            R  ++R     A+KKF  G+  ++LDP LEQ  ANNLA+EK+LE+A QCLAP R+SRPS
Sbjct: 336 SRGQKERITIRWAIKKFTSGDTISVLDPKLEQNSANNLALEKVLEMAFQCLAPHRRSRPS 395

Query: 427 MRRCAEILWSIRKDHREL 433
           M++C+EILW IRKD+REL
Sbjct: 396 MKKCSEILWGIRKDYREL 406

BLAST of MS024974 vs. TAIR 10
Match: AT5G58940.1 (calmodulin-binding receptor-like cytoplasmic kinase 1 )

HSP 1 Score: 346.7 bits (888), Expect = 2.9e-95
Identity = 185/340 (54.41%), Postives = 251/340 (73.82%), Query Frame = 0

Query: 117 RNQGSRRESHFSSNLAAHDREPGSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKL-D 176
           R  GSR +S    + ++     G +  S  E+++AT NFS+  +IG+GGFG V+KGKL D
Sbjct: 109 RFSGSRFQSPGKDSSSSKSWHQGPVIFSFGELQRATANFSSVHQIGEGGFGTVFKGKLDD 168

Query: 177 GMLVAIKRAKKSVYDNNLGLEFKSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNG 236
           G +VAIKRA+K+ Y  +  LEFK+EI TL+++EH+NLVK YG+LEH DE++++VEYV NG
Sbjct: 169 GTIVAIKRARKNNYGKSWLLEFKNEIYTLSKIEHMNLVKLYGFLEHGDEKVIVVEYVANG 228

Query: 237 TLREHLECIHGNILDLATRLDIATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAK 296
            LREHL+ + GN L++A RL+IA DVAHA+TYLH YTD PIIHRDIK+SNIL++   RAK
Sbjct: 229 NLREHLDGLRGNRLEMAERLEIAIDVAHALTYLHTYTDSPIIHRDIKASNILITNKLRAK 288

Query: 297 VADFGFARLAA-DGDATHVSTQVKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGR 356
           VADFGFARL + D  ATH+STQVKG+AGY+DP+YL+T+QLT+KSD+YSFGVLLVE++TGR
Sbjct: 289 VADFGFARLVSEDLGATHISTQVKGSAGYVDPDYLRTFQLTDKSDVYSFGVLLVEILTGR 348

Query: 357 RPIEPKRELEQR-----AMKKFAEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRR 416
           RPIE KR  + R     A+++  + EA  I+DP L++  A     EK+L LA +C+ P R
Sbjct: 349 RPIELKRPRKDRLTVKWALRRLKDDEAVLIMDPFLKRNRAAIEVAEKMLRLASECVTPTR 408

Query: 417 QSRPSMRRCAEILWSIRKDHRELSAPDLRSFSSNSQRSAS 450
            +RP+M+  AE LW+IR   RE+    + S +SNS  S++
Sbjct: 409 ATRPAMKGIAEKLWAIR---REMKETMICSSASNSSCSST 445

BLAST of MS024974 vs. TAIR 10
Match: AT2G11520.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 )

HSP 1 Score: 303.9 bits (777), Expect = 2.2e-82
Identity = 157/298 (52.68%), Postives = 208/298 (69.80%), Query Frame = 0

Query: 139 GSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKL-DGMLVAIKRAKKSVYDNNLGLEF 198
           G L +++ +I  AT NF+ + +IG+GGFG V+KG L DG +VAIKRAKK  ++ NL  EF
Sbjct: 209 GPLNLTMSQINTATGNFADSHQIGEGGFGVVFKGVLDDGQVVAIKRAKKEHFE-NLRTEF 268

Query: 199 KSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGNILDLATRLDI 258
           KSE+  L+++ H NLVK  GY++  DER++I EYV NGTLR+HL+   G  L+   RL+I
Sbjct: 269 KSEVDLLSKIGHRNLVKLLGYVDKGDERLIITEYVRNGTLRDHLDGARGTKLNFNQRLEI 328

Query: 259 ATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFGFARLA-ADGDATHVSTQ 318
             DV H +TYLH Y +R IIHRDIKSSNILL+++ RAKVADFGFAR    D + TH+ TQ
Sbjct: 329 VIDVCHGLTYLHSYAERQIIHRDIKSSNILLTDSMRAKVADFGFARGGPTDSNQTHILTQ 388

Query: 319 VKGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELEQR-----AMKKF 378
           VKGT GYLDPEY+KTY LT KSD+YSFG+LLVE++TGRRP+E KR  ++R     A  K+
Sbjct: 389 VKGTVGYLDPEYMKTYHLTAKSDVYSFGILLVEILTGRRPVEAKRLPDERITVRWAFDKY 448

Query: 379 AEGEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSMRRCAEILWSIRKDH 430
            EG    ++DPN  + V   + + K+  LA QC AP ++ RP M    + LW+IR  +
Sbjct: 449 NEGRVFELVDPNARERVDEKI-LRKMFSLAFQCAAPTKKERPDMEAVGKQLWAIRSSY 504

BLAST of MS024974 vs. TAIR 10
Match: AT5G49770.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 241.9 bits (616), Expect = 1.0e-63
Identity = 129/292 (44.18%), Postives = 184/292 (63.01%), Query Frame = 0

Query: 139 GSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKL-DGMLVAIKRAKKSVYDNNLGLEF 198
           G+   + +E+ K T NFS A+ +G GG+G VYKG L +G ++AIKRA++         EF
Sbjct: 618 GTKAFTFEELSKCTNNFSDANDVGGGGYGQVYKGTLPNGQVIAIKRAQQGSMQG--AFEF 677

Query: 199 KSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGNILDLATRLDI 258
           K+EI+ L++V H N+VK  G+   + E++++ EY+PNG+LR+ L   +G  LD   RL I
Sbjct: 678 KTEIELLSRVHHKNVVKLLGFCFDQKEQMLVYEYIPNGSLRDGLSGKNGVKLDWTRRLKI 737

Query: 259 ATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFGFARLAADGDATHVSTQV 318
           A      + YLH   D PIIHRD+KS+NILL E+  AKVADFG ++L  D +  HV+TQV
Sbjct: 738 ALGSGKGLAYLHELADPPIIHRDVKSNNILLDEHLTAKVADFGLSKLVGDPEKAHVTTQV 797

Query: 319 KGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIEPKRELEQRAMKKFAEG--- 378
           KGT GYLDPEY  T QLTEKSD+Y FGV+++EL+TG+ PI+    + +   KK  +    
Sbjct: 798 KGTMGYLDPEYYMTNQLTEKSDVYGFGVVMLELLTGKSPIDRGSYVVKEVKKKMDKSRNL 857

Query: 379 -EASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSMRRCAEILWSI 426
            +   +LD  + Q   N    EK +++ALQC+ P   +RP+M    + L SI
Sbjct: 858 YDLQELLDTTIIQNSGNLKGFEKYVDVALQCVEPEGVNRPTMSEVVQELESI 907

BLAST of MS024974 vs. TAIR 10
Match: AT5G49760.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 240.0 bits (611), Expect = 3.8e-63
Identity = 137/314 (43.63%), Postives = 197/314 (62.74%), Query Frame = 0

Query: 139 GSLKISIDEIRKATKNFSTASKIGQGGFGAVYKGKL-DGMLVAIKRAKKSVYDNNLGLEF 198
           G+   + +E++K T NFS A+ +G GG+G VY+G L +G L+AIKRA++       GLEF
Sbjct: 615 GAKAFTFEELKKCTDNFSEANDVGGGGYGKVYRGILPNGQLIAIKRAQQGSLQG--GLEF 674

Query: 199 KSEIQTLAQVEHLNLVKFYGYLEHEDERIVIVEYVPNGTLREHLECIHGNILDLATRLDI 258
           K+EI+ L++V H N+V+  G+    +E++++ EY+ NG+L++ L    G  LD   RL I
Sbjct: 675 KTEIELLSRVHHKNVVRLLGFCFDRNEQMLVYEYISNGSLKDSLSGKSGIRLDWTRRLKI 734

Query: 259 ATDVAHAITYLHMYTDRPIIHRDIKSSNILLSENFRAKVADFGFARLAADGDATHVSTQV 318
           A      + YLH   D PIIHRDIKS+NILL EN  AKVADFG ++L  D + THV+TQV
Sbjct: 735 ALGSGKGLAYLHELADPPIIHRDIKSNNILLDENLTAKVADFGLSKLVGDPEKTHVTTQV 794

Query: 319 KGTAGYLDPEYLKTYQLTEKSDIYSFGVLLVELVTGRRPIE----PKRELEQRAMKKFAE 378
           KGT GYLDPEY  T QLTEKSD+Y FGV+L+EL+TGR PIE      RE++ +  K  + 
Sbjct: 795 KGTMGYLDPEYYMTNQLTEKSDVYGFGVVLLELLTGRSPIERGKYVVREVKTKMNKSRSL 854

Query: 379 GEASTILDPNLEQTVANNLAVEKILELALQCLAPRRQSRPSMRRCAEILWSIRKDHRELS 438
            +   +LD  +  +  N    EK ++LAL+C+     +RPSM    + + +I     +L+
Sbjct: 855 YDLQELLDTTIIASSGNLKGFEKYVDLALRCVEEEGVNRPSMGEVVKEIENI----MQLA 914

Query: 439 APDLRSFSSNSQRS 448
             +  S S+ S R+
Sbjct: 915 GLNPNSDSATSSRT 922

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147253.12.0e-24798.26calmodulin-binding receptor-like cytoplasmic kinase 2 [Momordica charantia][more]
XP_023526304.19.0e-20884.19calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita pepo subsp. pep... [more]
KAG6582621.11.5e-20785.03Calmodulin-binding receptor-like cytoplasmic kinase 2, partial [Cucurbita argyro... [more]
XP_022924490.12.6e-20785.03calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita moschata][more]
KAG7019016.13.4e-20784.82Calmodulin-binding receptor-like cytoplasmic kinase 2 [Cucurbita argyrosperma su... [more]
Match NameE-valueIdentityDescription
Q8VZJ94.3e-12062.17Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Arabidopsis thaliana OX... [more]
Q9FIL74.1e-9454.41Calmodulin-binding receptor-like cytoplasmic kinase 1 OS=Arabidopsis thaliana OX... [more]
Q9ASQ53.0e-8152.68Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Arabidopsis thaliana OX... [more]
Q9LT961.4e-6244.18Probable leucine-rich repeat receptor-like protein kinase At5g49770 OS=Arabidops... [more]
Q8GZ995.4e-6243.63Leucine-rich repeat receptor protein kinase HPCA1 OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A6J1D0T19.6e-24898.26calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Momordica charantia OX=... [more]
A0A6J1EF631.3e-20785.03calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucurbita moschata OX=3... [more]
A0A6J1IRI15.0e-20484.16calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucurbita maxima OX=366... [more]
A0A5A7U1662.8e-20283.48Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucumis melo var. makuw... [more]
A0A1S3AVY62.8e-20283.48calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT4G00330.13.1e-12162.17calmodulin-binding receptor-like cytoplasmic kinase 2 [more]
AT5G58940.12.9e-9554.41calmodulin-binding receptor-like cytoplasmic kinase 1 [more]
AT2G11520.12.2e-8252.68calmodulin-binding receptor-like cytoplasmic kinase 3 [more]
AT5G49770.11.0e-6344.18Leucine-rich repeat protein kinase family protein [more]
AT5G49760.13.8e-6343.63Leucine-rich repeat protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 155..424
e-value: 1.1E-40
score: 151.2
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 156..420
e-value: 8.1E-52
score: 176.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 155..432
score: 40.555405
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 114..231
e-value: 3.8E-27
score: 96.3
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 232..445
e-value: 1.5E-57
score: 196.2
NoneNo IPR availablePIRSRPIRSR037281-2PIRSR037281-2coord: 129..295
e-value: 4.6E-15
score: 53.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 110..133
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 436..455
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 110..127
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..69
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..69
NoneNo IPR availablePANTHERPTHR45974RECEPTOR-LIKE PROTEIN 55coord: 36..442
NoneNo IPR availablePANTHERPTHR45974:SF34CALMODULIN-BINDING RECEPTOR-LIKE CYTOPLASMIC KINASE 2coord: 36..442
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 276..288
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 161..186
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 137..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS024974.1MS024974.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity