MS024886 (gene) Bitter gourd (TR) v1

Overview
NameMS024886
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionFatty acyl-CoA reductase
Locationscaffold176: 3156376 .. 3159585 (-)
RNA-Seq ExpressionMS024886
SyntenyMS024886
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTTCTCAAGAACAAGAACATTTTTGTCATGGGTGCCTCAGGCTTTCTGGGAAAGAGTATGTAATTATATATAAGTATTAATTTGTTTTGTTTTTTAAATTTATACCACTAATATTATCTAACGTGAGAGTGCAGTTATGGTAGAAAAAATATTAAGGGATCAACCAGACGTGAAGAAGCTTTATCTACTTTTACGAAGTGTTGATGAAAATATGGCAGCAAAACGTTTCTACGACGAGGTACGGCAACCCAATCTAGCAAAGATACATAATTTTTGTTTCAAAAAAAATTATTAAAAAGATTTATGACGTGTAGTATTTTATATGCAGGTCGTGGAGAGAGATCTGTTTAGTGTGTTAAAGGACAAATGGAATGAAAATTTGAACGCCTTCATTTCAGAGAAGATTTGTTTCGTGCCTGGTGATGTCTCGCTCCCGAATTTAGGGCTGAAAGACCCTAATTTATTGAAAGAGATGAAGGAGAAAGTGGAAATAATAGTTAATTTAGCTGCAACAGTCAATTTTGATGAAAGGTGCACAAATTCATGCATTTTCAGTAACAACAAATTAGAATAAAAAGTTCAAAGAAATTACCTTCCTCTTTATTGACATTGAATTTGTTTTTTTTTTTTTTTTTTGTGCAGATATGATGTGGCATTTGGCACTAATACATTGGGAGCTAAAAATGTATTAAGTTTTGCAAAGCAATGTTCTAATTTGGAGATTGTTGTCCATGTATCCACTGGTTAGTAATTACTATATAAAATATTCGCTAATTGAACCACTTACAAATGAAACAATACATATGTTTATAAAACGCTACCATTTTATTTATGTCTTTAACTTAGAGTTATGTTCAAGTTGGTAGTGCAAATATGATTAATCGCTAACTTAATCAACACTAACGGTATGATTTCTTTTTAAAATTTTAACTTTGAATAATCGACAAACGTTCGAGACAAATAAAATTTAATTATTGATGACTTGATTGCGGTATGATATTTTTAAAATAAAGAATTGAAAAATGTGTATTCTCTGGTAGCTTATGTTTCAGGTGAAAGGGAAGGAATTATTTTGGAAACCCCATTTGAGATTGGTGAATCGCTTAATGATACTGCTGGACTCAATATTGAGACAGAACAAAAATTGGTGGAGAAAACAATAGATGAACTAAAAACCAATGGATCTACAGAAGAAACTATAACTCTAACCATGAAGCACTTAGGCCTCGAAAGGTATTGAAAATTAGATGGCCAAGTCTTAATCAATTTAATAATATTTTTCAATAGTGATATGAAAAAGACAATAATTTTACTCAATTTCTTGTTTATTATAAGGGCTATGATGTACGGATGGCCAAATACGTATGTATTTACAAAGGCAATGGGAGAGATGGTGGTTGGTGACTTGAGAGATAATGTTCCTTTGGTTATCATACGACCAACCGTGATAACAAGTACTTATAAACAACCTTTTCCTGGTTGGGTCGAAGATATGAAGTATGTAATTTAACAATATTTTTGAATGTGGATTACCCAAAAAAATGAAATGTTCCAAATTATTGAAATTAACAGTTTTCTCGTCTTCCTTGAATTAAGGGGTGCGGACACTATTATGGTTGGTTACATTAAAGGAAAACTAACCGTTCTTCCGGTTGATATCAATTCAACCGTTGATTTAGTGAGTTCATATAATGATATAGTCAAGTCATTTGAGTTCGAAAAATACACATGTTTTGATTAAGTAAATAAATTATGGAATATACTTGAAAAATACAGATTCCGGCCGATATGGTGGTAAATGCAATGATTATGGCAATGATGACTCATGCACGTGAACCATGTCGTGTGATATACCATATTGGCTCTTCAACAAGAAATGGTATGACATGTGGGGATTTTTTGCATTTCACGTGGCAATACTTTGTTGAGAAGCCATGGATGAATCCAGATGGAAAAGCCATCAAAGTTGGGAAGATGACTGTCTTCAACAGCATGGTCAACTTTCGAAGATACACTGCCATTCGCTACTCCTTGCTTTTGAAGGTATATCATAGGCACTTTCCAAGTATCCAAAACCACTTGCAAACATTAACAAATCTAAACATTTTATTGGGATAACTACATCGACTCTAATTCTAAAATAACTTACACATTTGTCAAATTAGAGGATGAAGTCACATGAAAATTACCAAGCTACCTACGGTCATATATTTTTAATAAATAAATTACAAATATGAAAAGAAAAACCAATTTTAAGCCACCTCTGTTTATGATTTTTACACCTGAAACCCTAATCCCCTCTCTCCTTCTCTTCTCGCACTTTATAAAAATAGAAAAATATATGCCTATCGTACTATTAAAAATAAAACTCCAAAAAATATACATATATGTCGGAAAATTCAAACAAGAACGGCTGAAAAATTAAGAAATAATAAATTTAGAAATATAAAATCTTTAATCAAATATACATGGAAAGTATCAATAAAAATAAGCATAATAGATATGAAATGGTGGAATAAAAAAAAATCCTGAAAATCTAAAAGTAAAATTTTCATTTCACATTTGTGAAAAATAGGTCGTGACACAACCTTTAAAGACAATTTCTCAATTTTATTTTGGGGGTATGGGTATAATTGAATGCAGTAATAAGACAAATTATAAGAATGGAAAAATATATTATTCTCTTTGACCAAAAAAAAAAAAAAATTTGTATGCTTGAATTGATATCCGTTTTGTCGTTTGTACCATTGGCAAGGGCTTGGAATTTGCAAATGCCATATTTTGGGGTTCTTTCCAAGATACATGCAATAAGCACAGGAAGAAGTTCGATTCTATGATGAAACTCATGGAACTCTACCGTCCGTATCTGTTCTTCAAAGCCACGTATGTTACCATCTCTCGTTATAAACCACATTATATTTATTTAGGTGTGCTTTAGTATTGTTCGTTTCTCTTATTTTAGTTCTTTTTATTTCTACATCTAAATCTAAGTTTAGATGATTCATTTCAGATATCTTTCAGTTACTTATCTTCCTTTTTTCTTCTTCATACAGTTTTGACGATACAAATACAGAAAGATTGAGAATCACAGCAAGGAATTCAGACCTAGAAGAAACATTATACTTTGATCCTCGAGTAGTCGATTGGGCAGACTACTTTTACAATGTTCACATCCCTGCCGTGGTTAAATATGTCGTCACATGA

mRNA sequence

ATGGAGTTTCTCAAGAACAAGAACATTTTTGTCATGGGTGCCTCAGGCTTTCTGGGAAAGATTATGGTAGAAAAAATATTAAGGGATCAACCAGACGTGAAGAAGCTTTATCTACTTTTACGAAGTGTTGATGAAAATATGGCAGCAAAACGTTTCTACGACGAGGTCGTGGAGAGAGATCTGTTTAGTGTGTTAAAGGACAAATGGAATGAAAATTTGAACGCCTTCATTTCAGAGAAGATTTGTTTCGTGCCTGGTGATGTCTCGCTCCCGAATTTAGGGCTGAAAGACCCTAATTTATTGAAAGAGATGAAGGAGAAAGTGGAAATAATAGTTAATTTAGCTGCAACAGTCAATTTTGATGAAAGATATGATGTGGCATTTGGCACTAATACATTGGGAGCTAAAAATGTATTAAGTTTTGCAAAGCAATGTTCTAATTTGGAGATTGTTGTCCATGTATCCACTGGTGAAAGGGAAGGAATTATTTTGGAAACCCCATTTGAGATTGGTGAATCGCTTAATGATACTGCTGGACTCAATATTGAGACAGAACAAAAATTGGTGGAGAAAACAATAGATGAACTAAAAACCAATGGATCTACAGAAGAAACTATAACTCTAACCATGAAGCACTTAGGCCTCGAAAGGGCTATGATGTACGGATGGCCAAATACGTATGTATTTACAAAGGCAATGGGAGAGATGGTGGTTGGTGACTTGAGAGATAATGTTCCTTTGGTTATCATACGACCAACCGTGATAACAAGTACTTATAAACAACCTTTTCCTGGTTGGGTCGAAGATATGAAGGGTGCGGACACTATTATGGTTGGTTACATTAAAGGAAAACTAACCGTTCTTCCGGTTGATATCAATTCAACCGTTGATTTAATTCCGGCCGATATGGTGGTAAATGCAATGATTATGGCAATGATGACTCATGCACGTGAACCATGTCGTGTGATATACCATATTGGCTCTTCAACAAGAAATGGTATGACATGTGGGGATTTTTTGCATTTCACGTGGCAATACTTTGTTGAGAAGCCATGGATGAATCCAGATGGAAAAGCCATCAAAGTTGGGAAGATGACTGTCTTCAACAGCATGGTCAACTTTCGAAGATACACTGCCATTCGCTACTCCTTGCTTTTGAAGGGCTTGGAATTTGCAAATGCCATATTTTGGGGTTCTTTCCAAGATACATGCAATAAGCACAGGAAGAAGTTCGATTCTATGATGAAACTCATGGAACTCTACCGTCCGTATCTGTTCTTCAAAGCCACTTTTGACGATACAAATACAGAAAGATTGAGAATCACAGCAAGGAATTCAGACCTAGAAGAAACATTATACTTTGATCCTCGAGTAGTCGATTGGGCAGACTACTTTTACAATGTTCACATCCCTGCCGTGGTTAAATATGTCGTCACATGA

Coding sequence (CDS)

ATGGAGTTTCTCAAGAACAAGAACATTTTTGTCATGGGTGCCTCAGGCTTTCTGGGAAAGATTATGGTAGAAAAAATATTAAGGGATCAACCAGACGTGAAGAAGCTTTATCTACTTTTACGAAGTGTTGATGAAAATATGGCAGCAAAACGTTTCTACGACGAGGTCGTGGAGAGAGATCTGTTTAGTGTGTTAAAGGACAAATGGAATGAAAATTTGAACGCCTTCATTTCAGAGAAGATTTGTTTCGTGCCTGGTGATGTCTCGCTCCCGAATTTAGGGCTGAAAGACCCTAATTTATTGAAAGAGATGAAGGAGAAAGTGGAAATAATAGTTAATTTAGCTGCAACAGTCAATTTTGATGAAAGATATGATGTGGCATTTGGCACTAATACATTGGGAGCTAAAAATGTATTAAGTTTTGCAAAGCAATGTTCTAATTTGGAGATTGTTGTCCATGTATCCACTGGTGAAAGGGAAGGAATTATTTTGGAAACCCCATTTGAGATTGGTGAATCGCTTAATGATACTGCTGGACTCAATATTGAGACAGAACAAAAATTGGTGGAGAAAACAATAGATGAACTAAAAACCAATGGATCTACAGAAGAAACTATAACTCTAACCATGAAGCACTTAGGCCTCGAAAGGGCTATGATGTACGGATGGCCAAATACGTATGTATTTACAAAGGCAATGGGAGAGATGGTGGTTGGTGACTTGAGAGATAATGTTCCTTTGGTTATCATACGACCAACCGTGATAACAAGTACTTATAAACAACCTTTTCCTGGTTGGGTCGAAGATATGAAGGGTGCGGACACTATTATGGTTGGTTACATTAAAGGAAAACTAACCGTTCTTCCGGTTGATATCAATTCAACCGTTGATTTAATTCCGGCCGATATGGTGGTAAATGCAATGATTATGGCAATGATGACTCATGCACGTGAACCATGTCGTGTGATATACCATATTGGCTCTTCAACAAGAAATGGTATGACATGTGGGGATTTTTTGCATTTCACGTGGCAATACTTTGTTGAGAAGCCATGGATGAATCCAGATGGAAAAGCCATCAAAGTTGGGAAGATGACTGTCTTCAACAGCATGGTCAACTTTCGAAGATACACTGCCATTCGCTACTCCTTGCTTTTGAAGGGCTTGGAATTTGCAAATGCCATATTTTGGGGTTCTTTCCAAGATACATGCAATAAGCACAGGAAGAAGTTCGATTCTATGATGAAACTCATGGAACTCTACCGTCCGTATCTGTTCTTCAAAGCCACTTTTGACGATACAAATACAGAAAGATTGAGAATCACAGCAAGGAATTCAGACCTAGAAGAAACATTATACTTTGATCCTCGAGTAGTCGATTGGGCAGACTACTTTTACAATGTTCACATCCCTGCCGTGGTTAAATATGTCGTCACATGA

Protein sequence

MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERDLFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNFDERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVSTGEREGIILETPFEIGESLNDTAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEMVVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTVDLIPADMVVNAMIMAMMTHAREPCRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMNPDGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSMMKLMELYRPYLFFKATFDDTNTERLRITARNSDLEETLYFDPRVVDWADYFYNVHIPAVVKYVVT
Homology
BLAST of MS024886 vs. NCBI nr
Match: XP_022153083.1 (fatty acyl-CoA reductase 3-like [Momordica charantia])

HSP 1 Score: 619.4 bits (1596), Expect = 2.6e-173
Identity = 303/485 (62.47%), Postives = 381/485 (78.56%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           +EFL+NK+I + GA+GFL KI+VEKILR QP+VKKLYLLLRS DE  A KRF++EV+E+D
Sbjct: 10  IEFLENKSILITGATGFLAKILVEKILRTQPNVKKLYLLLRSSDEISAVKRFHNEVIEKD 69

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNF 120
           LF VL+ KW  NL+A ISEK+C VPG++SLP LG+KD   ++EMK KVEIIVNLAAT NF
Sbjct: 70  LFQVLRKKWGANLSALISEKVCVVPGEISLPCLGMKDSIRVEEMKRKVEIIVNLAATTNF 129

Query: 121 DERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLND 180
           DERYDVA GTNT+GA+N +SFAKQC NL+++VHVST    GEREG+ILETP+++GESLN 
Sbjct: 130 DERYDVALGTNTVGARNAVSFAKQCCNLKLLVHVSTAYVSGEREGLILETPYKLGESLNG 189

Query: 181 TAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEM 240
             GL+IETEQK+VE+ + +LK NG+T E ITL MK LGLERA MYGWPNTYVFTKAMGEM
Sbjct: 190 IKGLDIETEQKVVEERLKQLKENGATNEYITLAMKDLGLERAKMYGWPNTYVFTKAMGEM 249

Query: 241 VVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTV 300
           +V D +D++ L+IIRPT++TSTYK+PFPGW+E ++  D+I +GY KGKLT      NS +
Sbjct: 250 LVSDQKDDLSLIIIRPTIVTSTYKEPFPGWIEGVRTIDSITLGYAKGKLTCFLAGTNSII 309

Query: 301 DLIPADMVVNAMIMAMMTHAREPC-RVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMNP 360
           DLIPADMVVN ++M+M+ H   P  + IYH+GSS RN M   DF  F +QYF + PW+N 
Sbjct: 310 DLIPADMVVNTILMSMVAHELHPSNQTIYHVGSSARNSMRNIDFQRFNFQYFTKNPWINR 369

Query: 361 DGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSMM 420
           DG  +KVGK+TVF SM +FRRY AIRY   LKGLEFAN  F  SFQD   + ++KF+ +M
Sbjct: 370 DGNVVKVGKVTVFESMTSFRRYIAIRYLTFLKGLEFANKAFCHSFQDKYVEMKRKFNLVM 429

Query: 421 KLMELYRPYLFFKATFDDTNTERLRITARNSDLE-ETLYF-DPRVVDWADYFYNVHIPAV 479
           +L+ELYRPYLFF A FDDTN ERLR   +N D + E L+F DP+ ++W DYF NVH+P +
Sbjct: 430 RLIELYRPYLFFNAVFDDTNAERLRKDIQNKDTKTEALFFMDPKAINWEDYFMNVHLPGL 489

BLAST of MS024886 vs. NCBI nr
Match: XP_038902167.1 (fatty acyl-CoA reductase 3-like [Benincasa hispida])

HSP 1 Score: 619.0 bits (1595), Expect = 3.4e-173
Identity = 301/483 (62.32%), Postives = 383/483 (79.30%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           MEFL+NK+I + GA+GFL KI+VEKILR QP+VKKLYLLLR+ DE +A KRFY+EV+E+D
Sbjct: 1   MEFLENKSILITGATGFLAKILVEKILRVQPNVKKLYLLLRADDEIIAKKRFYNEVIEKD 60

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNF 120
           LF VLK K+  +LN  ISEKIC VPG++S P +GLKD      +K +VEII+NLAAT NF
Sbjct: 61  LFQVLKKKYGADLNTLISEKICLVPGEISFPQMGLKDSIWNTMIKNEVEIIINLAATTNF 120

Query: 121 DERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLND 180
           +ERYDV+ GTNTLGAK+V++FAKQCSNL+++VHVST    GEREG+I+ETP ++GESLN 
Sbjct: 121 NERYDVSLGTNTLGAKHVVNFAKQCSNLKLLVHVSTAYVSGEREGLIMETPHKLGESLNG 180

Query: 181 TAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEM 240
             GL+IE EQ +VE+ + +LK +G+T ETITL MK LGLER+ +YGWPNTYVFTKAMGEM
Sbjct: 181 NKGLDIEGEQMVVEEKLKQLKESGATNETITLAMKDLGLERSKLYGWPNTYVFTKAMGEM 240

Query: 241 VVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTV 300
           ++GDL+D++PL+IIRPT++TSTYK+PF GWVE ++  D+++VGY KGKLT     INS +
Sbjct: 241 IIGDLKDDLPLIIIRPTIVTSTYKEPFSGWVEGVRTIDSLIVGYAKGKLTCFVAGINSII 300

Query: 301 DLIPADMVVNAMIMAMMTHAREPC-RVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMNP 360
           DLIPADMVVN +IMAM  H  +P  + IYH+GSSTRN M   DF  F +QYF +KP ++ 
Sbjct: 301 DLIPADMVVNTIIMAMKVHKFQPSNQTIYHVGSSTRNSMKHIDFQRFNYQYFTKKPLIDR 360

Query: 361 DGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSMM 420
           DG AIKVGK+T+FN+M NFRRY AIRY + LKGLE AN  F  SFQD     ++KF+ +M
Sbjct: 361 DGNAIKVGKVTIFNNMTNFRRYIAIRYLIFLKGLEIANMAFCHSFQDKYINMKRKFNLVM 420

Query: 421 KLMELYRPYLFFKATFDDTNTERLRITARNSDLEETLYFDPRVVDWADYFYNVHIPAVVK 479
           +L+ELYRPYLFF A FDDTNTERLR   +N D +E L+ DP+ ++W DYF NVHIP +VK
Sbjct: 421 RLIELYRPYLFFNAIFDDTNTERLRKDIQNKDTKEALFMDPKDINWEDYFLNVHIPGLVK 480

BLAST of MS024886 vs. NCBI nr
Match: XP_022153045.1 (fatty acyl-CoA reductase 3-like isoform X2 [Momordica charantia])

HSP 1 Score: 618.2 bits (1593), Expect = 5.8e-173
Identity = 303/479 (63.26%), Postives = 385/479 (80.38%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           MEFL+NK++F++GA GFLGKIMVEKILR QP+VKKLY+L+R+ DE  AA+RFY EVV +D
Sbjct: 1   MEFLENKSVFIIGALGFLGKIMVEKILRVQPNVKKLYILVRTTDEITAAQRFYYEVVGKD 60

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNF 120
           LF+ LKDKW  +LN  ISEK+  V GD+SLPN+GLKD NLL+EMK +VEIIVNLAAT  F
Sbjct: 61  LFTRLKDKWRGDLNTLISEKVRLVAGDISLPNMGLKDSNLLEEMKNQVEIIVNLAATTKF 120

Query: 121 DERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVSTGEREGIILETPFEIGESLNDTAGL 180
            ERYDVAFGTNTLGAK+VL FAKQCSNL+++VHVSTGE++G+ILETP+E+G+SL  T  L
Sbjct: 121 YERYDVAFGTNTLGAKHVLGFAKQCSNLQVLVHVSTGEKKGLILETPYEMGKSLYQTTSL 180

Query: 181 NIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEMVVGD 240
           NIETE+ +VE+T++EL+ NG TEE  TL MK LGL+RAMM+GWPNTYVFTKAMGEMVV D
Sbjct: 181 NIETERIVVEETLNELRNNGGTEERTTLAMKQLGLKRAMMHGWPNTYVFTKAMGEMVVSD 240

Query: 241 LRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTVDLIP 300
            +D++PLVIIRPTVITST K+PFPGW+E ++  D+++VGY KGKLT +P D NS +D+IP
Sbjct: 241 QKDHLPLVIIRPTVITSTCKEPFPGWIEGVRTIDSLIVGYAKGKLTSIPCDNNSILDVIP 300

Query: 301 ADMVVNAMIMAMMTHAREP-CRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMNPDGKA 360
           ADMVVN ++MA++ H  +P  + IYH+GSS RN M   D  +F +QYF+EKPW++ DG A
Sbjct: 301 ADMVVNTIVMAIVAHKLQPSSQRIYHVGSSLRNSMRYIDLKNFLFQYFIEKPWIDEDGNA 360

Query: 361 IKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSMMKLME 420
           IKV K+ VFN+M +FRR+ +IRY + LK LEFAN     S +D     ++KFD M++L E
Sbjct: 361 IKVKKIIVFNNMASFRRHMSIRYLIFLKVLEFANKASCHSLEDLYADLKRKFDWMVRLTE 420

Query: 421 LYRPYLFFKATFDDTNTERLRITARNSDLE-ETLYFDPRVVDWADYFYNVHIPAVVKYV 478
           LYRPYLFF A FDDTN E+LRI  ++ D +  TL+ DPR ++W DYF N+HIP +VK+V
Sbjct: 421 LYRPYLFFDARFDDTNLEKLRIATQDIDRDANTLFLDPRDINWEDYFVNIHIPGLVKHV 479

BLAST of MS024886 vs. NCBI nr
Match: XP_022943504.1 (fatty acyl-CoA reductase 3-like [Cucurbita moschata])

HSP 1 Score: 614.8 bits (1584), Expect = 6.4e-172
Identity = 296/483 (61.28%), Postives = 380/483 (78.67%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           MEFL+NKNI + GA+GFL KI+VEKILR QP+VKKLYLLLR+ +E+ A +RF++EV+ +D
Sbjct: 8   MEFLENKNILITGATGFLAKILVEKILRVQPNVKKLYLLLRAANESTALERFHNEVIGKD 67

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNF 120
           LF VLK  W  + +  ISEK+C VPG+VSL  +GLKD NL+ EMK +VE+IVNLAAT  F
Sbjct: 68  LFQVLKKMWGRDFDTLISEKVCVVPGEVSLSEIGLKDSNLVAEMKNQVELIVNLAATTKF 127

Query: 121 DERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLND 180
           DERYDVA GTNTLGAK+V+SFAKQC NL+++VHVST    GER G+ILETP+++GESLN 
Sbjct: 128 DERYDVAVGTNTLGAKHVVSFAKQCPNLKLLVHVSTAYVSGERGGLILETPYKLGESLNG 187

Query: 181 TAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEM 240
             GL+I TEQK++E+ +++L+ NG+T+E ITL MK  GLERA MYGWPNTYVFTKAMGEM
Sbjct: 188 IQGLDIATEQKVMEEKLEQLEENGATKEAITLAMKDFGLERAKMYGWPNTYVFTKAMGEM 247

Query: 241 VVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTV 300
           +V DL+DN+P++IIRPT++TSTYK+PFPGW+E ++  D+++VGY KGKLT     INS V
Sbjct: 248 MVNDLKDNLPVIIIRPTIVTSTYKEPFPGWIEGIRTIDSLIVGYAKGKLTCFLAGINSIV 307

Query: 301 DLIPADMVVNAMIMAMMTHAREPC-RVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMNP 360
           DL+PADMVVN +IMAM+ H  +P  + IYH+GSS RN M   DF  F +QYF +KPW+N 
Sbjct: 308 DLVPADMVVNTIIMAMVAHELQPSDKAIYHVGSSARNSMRYIDFQRFNYQYFTKKPWINK 367

Query: 361 DGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSMM 420
           +G A+KVGK+TV+N+M +F+RY  IRY  LLKGLEFAN  F  SFQD     ++KF+ +M
Sbjct: 368 EGNAVKVGKVTVYNNMASFQRYMNIRYLCLLKGLEFANKAFCHSFQDKYVDMKRKFNLVM 427

Query: 421 KLMELYRPYLFFKATFDDTNTERLRITARNSDLE-ETLYFDPRVVDWADYFYNVHIPAVV 478
           +L++LYRPYLFF A FDDTN ERLR+     D E ET   DP+ ++W DYF NVH+P +V
Sbjct: 428 RLIQLYRPYLFFNAIFDDTNVERLRMDIEKKDREPETFLLDPKEINWEDYFMNVHLPGLV 487

BLAST of MS024886 vs. NCBI nr
Match: KAG6570488.1 (Fatty acyl-CoA reductase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 614.8 bits (1584), Expect = 6.4e-172
Identity = 296/483 (61.28%), Postives = 380/483 (78.67%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           MEFL+NKNI + GA+GFL KI+VEKILR QP+VKKLYLLLR+ +E+ A +RF++EV+ +D
Sbjct: 8   MEFLENKNILITGATGFLAKILVEKILRVQPNVKKLYLLLRAANESTALERFHNEVIGKD 67

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNF 120
           LF VLK  W  + +  ISEK+C VPG+VSL  +GLKD NL+ EMK +VE+IVNLAAT  F
Sbjct: 68  LFQVLKKMWGRDFDTLISEKVCVVPGEVSLSEMGLKDSNLVAEMKNQVELIVNLAATTKF 127

Query: 121 DERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLND 180
           DERYDVA GTNTLGAK+V+SFAKQC NL+++VHVST    GER G+ILETP+++GESLN 
Sbjct: 128 DERYDVAVGTNTLGAKHVVSFAKQCPNLKLLVHVSTAYVSGERGGLILETPYKLGESLNG 187

Query: 181 TAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEM 240
             GL+I TEQK++E+ +++L+ NG+T+E ITL MK  GLERA MYGWPNTYVFTKAMGEM
Sbjct: 188 IQGLDIATEQKVMEEKLEQLEENGATKEAITLAMKDFGLERAKMYGWPNTYVFTKAMGEM 247

Query: 241 VVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTV 300
           +V DL+DN+P++IIRPT++TSTYK+PFPGW+E ++  D+++VGY KGKLT     INS V
Sbjct: 248 MVNDLKDNLPVIIIRPTIVTSTYKEPFPGWIEGIRTIDSLIVGYAKGKLTCFLAGINSIV 307

Query: 301 DLIPADMVVNAMIMAMMTHAREPC-RVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMNP 360
           DL+PADMVVN +IMAM+ H  +P  + IYH+GSS RN M   DF  F +QYF +KPW+N 
Sbjct: 308 DLVPADMVVNTIIMAMVAHELQPSDKAIYHVGSSARNSMRYIDFQRFNYQYFTKKPWINK 367

Query: 361 DGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSMM 420
           +G A+KVGK+TV+N+M +F+RY  IRY  LLKGLEFAN  F  SFQD     ++KF+ +M
Sbjct: 368 EGNAVKVGKVTVYNNMASFQRYMNIRYLCLLKGLEFANKAFCHSFQDKYVDMKRKFNLVM 427

Query: 421 KLMELYRPYLFFKATFDDTNTERLRITARNSDLE-ETLYFDPRVVDWADYFYNVHIPAVV 478
           +L++LYRPYLFF A FDDTN ERLR+     D E ET   DP+ ++W DYF NVH+P +V
Sbjct: 428 RLIQLYRPYLFFNAIFDDTNVERLRMDIEKKDREPETFLLDPKEINWEDYFMNVHLPGLV 487

BLAST of MS024886 vs. ExPASy Swiss-Prot
Match: Q93ZB9 (Fatty acyl-CoA reductase 3 OS=Arabidopsis thaliana OX=3702 GN=FAR3 PE=1 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 5.3e-145
Identity = 254/482 (52.70%), Postives = 346/482 (71.78%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           +++L NK+I V+GA+GFL  I VEKILR  P+VKKLYLLLR+     A +RF DE++++D
Sbjct: 11  LKYLDNKSILVVGAAGFLANIFVEKILRVAPNVKKLYLLLRASKGKSATQRFNDEILKKD 70

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNF 120
           LF VLK+K+  NLN   SEKI  V GD+ L +LGL+D +L  EM  +V+ IVNLAAT  F
Sbjct: 71  LFKVLKEKYGPNLNQLTSEKITIVDGDICLEDLGLQDFDLAHEMIHQVDAIVNLAATTKF 130

Query: 121 DERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLND 180
           DERYDVA G NTLGA NVL+FAK+C+ ++I+VHVST    GE+ G+I+ETP+ +GE+LN 
Sbjct: 131 DERYDVALGINTLGALNVLNFAKRCAKVKILVHVSTAYVCGEKSGLIMETPYRMGETLNG 190

Query: 181 TAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEM 240
           T GL+I  E+KLV++ +D+L+  G+  ETIT TMK LGL RA MYGWPNTYVFTKAMGEM
Sbjct: 191 TTGLDINYEKKLVQEKLDQLRVIGAAPETITETMKDLGLRRAKMYGWPNTYVFTKAMGEM 250

Query: 241 VVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTV 300
           +VG  R+N+ LV++RP++ITST+K+PFPGW E ++  D++ VGY KGKLT    D+++  
Sbjct: 251 MVGTKRENLSLVLLRPSIITSTFKEPFPGWTEGIRTIDSLAVGYGKGKLTCFLCDLDAVS 310

Query: 301 DLIPADMVVNAMIMAMMTHAREPCRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMNPD 360
           D++PADMVVN+++++M   A +   +IYH+GSS RN M    F    ++YF  KPW N +
Sbjct: 311 DVMPADMVVNSILVSMAAQAGKQEEIIYHVGSSLRNPMKNSKFPELAYRYFSIKPWTNKE 370

Query: 361 GKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSMMK 420
           GK +KVG + + +SM +F RY  IRY + LKGLE  N I    F+       KK + + +
Sbjct: 371 GKVVKVGAIEILSSMRSFHRYMTIRYLIALKGLELVNIILCKLFEKEFQYFNKKINFIFR 430

Query: 421 LMELYRPYLFFKATFDDTNTERLRITARNSDLE-ETLYFDPRVVDWADYFYNVHIPAVVK 478
           L++LY+PYLFF   FDD+NTE+LR     + +E E  YFDP+V+DW DYF N H+  ++K
Sbjct: 431 LVDLYQPYLFFYGIFDDSNTEKLRKMVSKTGVENEMFYFDPKVLDWDDYFLNTHVIGLLK 490

BLAST of MS024886 vs. ExPASy Swiss-Prot
Match: Q9XGY7 (Alcohol-forming fatty acyl-CoA reductase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 3.6e-133
Identity = 244/487 (50.10%), Postives = 332/487 (68.17%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           +EFL NK I V GA+G L KI VEK+LR QP+VKKLYLLLR+ D+  AA R  +EV  ++
Sbjct: 8   LEFLDNKAILVTGATGSLAKIFVEKVLRSQPNVKKLYLLLRATDDETAALRLQNEVFGKE 67

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNF 120
           LF VLK     N  +F+SEK+  VPGD++  +L LKD NL +EM  +++++VNLAAT+NF
Sbjct: 68  LFKVLKQNLGANFYSFVSEKVTVVPGDITGEDLCLKDVNLKEEMWREIDVVVNLAATINF 127

Query: 121 DERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLND 180
            ERYDV+   NT GAK VL FAK+C+ L+I VHVST    GE+ G+ILE P+ +GESLN 
Sbjct: 128 IERYDVSLLINTYGAKYVLDFAKKCNKLKIFVHVSTAYVSGEKNGLILEKPYYMGESLNG 187

Query: 181 TAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEM 240
             GL+I  E+KLVE  I+EL+  G+TE++I  TMK +G+ERA  +GWPN YVFTKA+GEM
Sbjct: 188 RLGLDINVEKKLVEAKINELQAAGATEKSIKSTMKDMGIERARHWGWPNVYVFTKALGEM 247

Query: 241 VVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTV 300
           ++   + ++PL IIRPT+ITST+K+PFPGWVE ++  D + V Y KG+L  +    ++ +
Sbjct: 248 LLMQYKGDIPLTIIRPTIITSTFKEPFPGWVEGVRTIDNVPVYYGKGRLRCMLCGPSTII 307

Query: 301 DLIPADMVVNAMIMAMMTHAR----EPCRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPW 360
           DLIPADMVVNA I+AM+ HA     EP  V YH+GSS  N M          +YF + PW
Sbjct: 308 DLIPADMVVNATIVAMVAHANQRYVEP--VTYHVGSSAANPMKLSALPEMAHRYFTKNPW 367

Query: 361 MNPDGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFD 420
           +NPD   + VG+  VF+S   F  Y  + + L LK LE AN IF   F+      ++K  
Sbjct: 368 INPDRNPVHVGRAMVFSSFSTFHLYLTLNFLLPLKVLEIANTIFCQWFKGKYMDLKRKTR 427

Query: 421 SMMKLMELYRPYLFFKATFDDTNTERLRITARNSDLE-ETLYFDPRVVDWADYFYNVHIP 479
            +++L+++Y+PYLFF+  FDD NTE+LRI A+ S +E +  YFDPR ++W DYF   H P
Sbjct: 428 LLLRLVDIYKPYLFFQGIFDDMNTEKLRIAAKESIVEADMFYFDPRAINWEDYFLKTHFP 487

BLAST of MS024886 vs. ExPASy Swiss-Prot
Match: Q39152 (Fatty acyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=FAR1 PE=1 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 3.5e-128
Identity = 235/486 (48.35%), Postives = 333/486 (68.52%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           ++FL NK I + GA GFL K++VEKILR QP+VKK+YLLLR+ DE  A +R   EV+E D
Sbjct: 6   VQFLGNKTILITGAPGFLAKVLVEKILRLQPNVKKIYLLLRAPDEKSAMQRLRSEVMEID 65

Query: 61  LFSVLKDKWNE-NLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVN 120
           LF VL++   E NLNA + EKI  VPGD+S+ NLGLKD +L++ M  +++II+N+AAT N
Sbjct: 66  LFKVLRNNLGEDNLNALMREKIVPVPGDISIDNLGLKDTDLIQRMWSEIDIIINIAATTN 125

Query: 121 FDERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLN 180
           FDERYD+  G NT GA NVL+FAK+C   ++++HVST    GE+ G++LE PF++GE+L+
Sbjct: 126 FDERYDIGLGINTFGALNVLNFAKKCVKGQLLLHVSTAYISGEQPGLLLEKPFKMGETLS 185

Query: 181 DTAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGE 240
               L+I  E  L+++ + EL+    ++E I+ TMK  G+ RA ++GWPNTYVFTKAMGE
Sbjct: 186 GDRELDINIEHDLMKQKLKELQ--DCSDEEISQTMKDFGMARAKLHGWPNTYVFTKAMGE 245

Query: 241 MVVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINST 300
           M++G  R+N+PLVIIRPT+ITST  +PFPGW+E +K  D+++V Y KG+L     D NS 
Sbjct: 246 MLMGKYRENLPLVIIRPTMITSTIAEPFPGWIEGLKTLDSVIVAYGKGRLKCFLADSNSV 305

Query: 301 VDLIPADMVVNAMIMAMMTHAREP-CRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMN 360
            DLIPADMVVNAM+ A   H+ +   + IYH+GSS +N +T G    FT +YF ++P + 
Sbjct: 306 FDLIPADMVVNAMVAAATAHSGDTGIQAIYHVGSSCKNPVTFGQLHDFTARYFAKRPLIG 365

Query: 361 PDGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSM 420
            +G  I V K T+ ++M  F  Y  +RY L L+ L   N ++  S  D  +   +K    
Sbjct: 366 RNGSPIIVVKGTILSTMAQFSLYMTLRYKLPLQILRLINIVYPWSHGDNYSDLSRKIKLA 425

Query: 421 MKLMELYRPYLFFKATFDDTNTERLRITARNS--DLEETLYFDPRVVDWADYFYNVHIPA 479
           M+L+ELY+PYL FK  FDD NTERLR+  + +  +L+ +  FDP+ +DW +Y  N HIP 
Sbjct: 426 MRLVELYQPYLLFKGIFDDLNTERLRMKRKENIKELDGSFEFDPKSIDWDNYITNTHIPG 485

BLAST of MS024886 vs. ExPASy Swiss-Prot
Match: Q9LXN3 (Probable fatty acyl-CoA reductase 4 OS=Arabidopsis thaliana OX=3702 GN=FAR4 PE=1 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 8.0e-125
Identity = 229/491 (46.64%), Postives = 328/491 (66.80%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           ++FL +K I V G  GFL K+ VEKILR QP VKKL+LLLR+ D   A +RF+ EV+E+D
Sbjct: 6   IQFLHDKTILVTGVPGFLAKVFVEKILRIQPKVKKLFLLLRAADNESAMQRFHSEVLEKD 65

Query: 61  LFSVLKDK-WNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVN 120
           LF VLK+   +ENL AFI+EK+  +PGD+S+ NLG+K  +LL+ M  +++IIVN+AAT N
Sbjct: 66  LFRVLKNALGDENLKAFITEKVVPIPGDISVDNLGVKGSDLLQHMWNEIDIIVNVAATTN 125

Query: 121 FDERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLN 180
           FDERYDV    NT G  NVL+FAK+C   ++++HVST    GE+ G++ E  F +GE+LN
Sbjct: 126 FDERYDVGLSVNTFGPLNVLNFAKKCVKGQLLLHVSTAYVRGEKSGLLHEKTFHMGETLN 185

Query: 181 DTAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGE 240
               L IETE +L+++ + EL+    +EE I+ +MK LG+ RA ++GWPNTYVFTK+MGE
Sbjct: 186 GHRKLVIETEMELMKQKLKELQKQNCSEEEISQSMKDLGMSRAKLHGWPNTYVFTKSMGE 245

Query: 241 MVVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINST 300
           M++G+ R+N+P+VIIRPT+ITST+ +PFPGW+E ++  D+++V Y KG+L     D NS 
Sbjct: 246 MLLGNYRENLPIVIIRPTMITSTFSEPFPGWIEGLRTIDSVIVAYGKGRLKCFLADPNSV 305

Query: 301 VDLIPADMVVNAMIMAMMTHA-REPCRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMN 360
           +DLIP DMV NAM+ A   HA +   + +YH+GSS +N +T          YF + P + 
Sbjct: 306 LDLIPVDMVANAMVTAAAIHAGKLGSQTVYHVGSSCKNPITFEQIHDLAASYFTKNPLVR 365

Query: 361 PDGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIF--WGSFQDTCNKHR---K 420
            DG +I V K T+ ++M  F  Y  +RY L L+ L     I+  W       NK++   +
Sbjct: 366 RDGSSILVSKGTILSTMAQFSFYMTLRYKLPLQMLRLIYVIYPWWNG-----NKYKDIDR 425

Query: 421 KFDSMMKLMELYRPYLFFKATFDDTNTERLRITAR--NSDLEETLYFDPRVVDWADYFYN 479
           K    M+L++LYRPY+ FK  FDDTNTE+LR+  +  N ++     FDP+ +DW DY   
Sbjct: 426 KIKLAMRLVDLYRPYVLFKGIFDDTNTEKLRLKRKEINKEMYGLFEFDPKSIDWEDYMTT 485

BLAST of MS024886 vs. ExPASy Swiss-Prot
Match: Q0WRB0 (Probable fatty acyl-CoA reductase 5 OS=Arabidopsis thaliana OX=3702 GN=FAR5 PE=1 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 3.9e-119
Identity = 219/489 (44.79%), Postives = 320/489 (65.44%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           ++FL+NK I V GA+GFL K+ VEKILR QP+VKKLYLL+R+ D   A KR   EV E++
Sbjct: 6   VQFLRNKTILVTGATGFLAKVFVEKILRVQPNVKKLYLLVRASDNEAATKRLRTEVFEKE 65

Query: 61  LFSVLKDK-WNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVN 120
           LF VL+    +E LN  + EK+  VPGD++   LG+ D +L + M+++++I+VN+AAT N
Sbjct: 66  LFKVLRQNLGDEKLNTLLYEKVVSVPGDIATDQLGINDSHLRERMQKEIDIVVNVAATTN 125

Query: 121 FDERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLN 180
           FDERYDV  G NT GA NVL+FAK+C  +++++HVST    GE+ G+I E PF + E  N
Sbjct: 126 FDERYDVGLGINTFGALNVLNFAKKCVKVQLLLHVSTAYVCGEKPGLIPEKPFIMEEIRN 185

Query: 181 DTA-GLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMG 240
           +    L+I  E++L+++ + EL     +EE ITL+MK LG+ERA ++GWPNTYVFTK+MG
Sbjct: 186 ENGLQLDINLERELMKQRLKELNEQDCSEEDITLSMKELGMERAKLHGWPNTYVFTKSMG 245

Query: 241 EMVVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINS 300
           EM++G  ++N+PLVIIRPT+ITST  +PFPGW+E ++  D++++ Y KG L    VD+NS
Sbjct: 246 EMLLGKHKENLPLVIIRPTMITSTLSEPFPGWIEGLRTVDSVIIAYGKGVLKCFLVDVNS 305

Query: 301 TVDLIPADMVVNAMIMAMMTHA-REPCRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWM 360
             D+IP DMV NAMI A   HA      ++YH+GSS +N +T G+      +YF + P  
Sbjct: 306 VCDMIPVDMVANAMITAAAKHAGGSGVHMVYHVGSSHQNPVTFGEIHEIAVRYFTKNPLR 365

Query: 361 NPDGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDS 420
           + +G  I V K+    +M  F  Y  +RY L L+ L+  + I+     D      +K + 
Sbjct: 366 SRNGSLITVSKVRFIPTMALFSLYMTLRYKLPLQLLKLVDIIYPWRNGDKYGDKNRKIEL 425

Query: 421 MMKLMELYRPYLFFKATFDDTNTERLRITARNSDLEET----LYFDPRVVDWADYFYNVH 479
           +M+L+ELY PY+ FK  FDD NT+ L    +  +++ T      FDP+ ++W DY  N+H
Sbjct: 426 VMRLVELYEPYVLFKGIFDDRNTKSLCANQKEEEIKNTEKLMFDFDPKGINWGDYLTNIH 485

BLAST of MS024886 vs. ExPASy TrEMBL
Match: A0A6J1DGK4 (Fatty acyl-CoA reductase OS=Momordica charantia OX=3673 GN=LOC111020669 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 1.3e-173
Identity = 303/485 (62.47%), Postives = 381/485 (78.56%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           +EFL+NK+I + GA+GFL KI+VEKILR QP+VKKLYLLLRS DE  A KRF++EV+E+D
Sbjct: 10  IEFLENKSILITGATGFLAKILVEKILRTQPNVKKLYLLLRSSDEISAVKRFHNEVIEKD 69

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNF 120
           LF VL+ KW  NL+A ISEK+C VPG++SLP LG+KD   ++EMK KVEIIVNLAAT NF
Sbjct: 70  LFQVLRKKWGANLSALISEKVCVVPGEISLPCLGMKDSIRVEEMKRKVEIIVNLAATTNF 129

Query: 121 DERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLND 180
           DERYDVA GTNT+GA+N +SFAKQC NL+++VHVST    GEREG+ILETP+++GESLN 
Sbjct: 130 DERYDVALGTNTVGARNAVSFAKQCCNLKLLVHVSTAYVSGEREGLILETPYKLGESLNG 189

Query: 181 TAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEM 240
             GL+IETEQK+VE+ + +LK NG+T E ITL MK LGLERA MYGWPNTYVFTKAMGEM
Sbjct: 190 IKGLDIETEQKVVEERLKQLKENGATNEYITLAMKDLGLERAKMYGWPNTYVFTKAMGEM 249

Query: 241 VVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTV 300
           +V D +D++ L+IIRPT++TSTYK+PFPGW+E ++  D+I +GY KGKLT      NS +
Sbjct: 250 LVSDQKDDLSLIIIRPTIVTSTYKEPFPGWIEGVRTIDSITLGYAKGKLTCFLAGTNSII 309

Query: 301 DLIPADMVVNAMIMAMMTHAREPC-RVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMNP 360
           DLIPADMVVN ++M+M+ H   P  + IYH+GSS RN M   DF  F +QYF + PW+N 
Sbjct: 310 DLIPADMVVNTILMSMVAHELHPSNQTIYHVGSSARNSMRNIDFQRFNFQYFTKNPWINR 369

Query: 361 DGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSMM 420
           DG  +KVGK+TVF SM +FRRY AIRY   LKGLEFAN  F  SFQD   + ++KF+ +M
Sbjct: 370 DGNVVKVGKVTVFESMTSFRRYIAIRYLTFLKGLEFANKAFCHSFQDKYVEMKRKFNLVM 429

Query: 421 KLMELYRPYLFFKATFDDTNTERLRITARNSDLE-ETLYF-DPRVVDWADYFYNVHIPAV 479
           +L+ELYRPYLFF A FDDTN ERLR   +N D + E L+F DP+ ++W DYF NVH+P +
Sbjct: 430 RLIELYRPYLFFNAVFDDTNAERLRKDIQNKDTKTEALFFMDPKAINWEDYFMNVHLPGL 489

BLAST of MS024886 vs. ExPASy TrEMBL
Match: A0A6J1DFP2 (Fatty acyl-CoA reductase OS=Momordica charantia OX=3673 GN=LOC111020641 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.8e-173
Identity = 303/479 (63.26%), Postives = 385/479 (80.38%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           MEFL+NK++F++GA GFLGKIMVEKILR QP+VKKLY+L+R+ DE  AA+RFY EVV +D
Sbjct: 1   MEFLENKSVFIIGALGFLGKIMVEKILRVQPNVKKLYILVRTTDEITAAQRFYYEVVGKD 60

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNF 120
           LF+ LKDKW  +LN  ISEK+  V GD+SLPN+GLKD NLL+EMK +VEIIVNLAAT  F
Sbjct: 61  LFTRLKDKWRGDLNTLISEKVRLVAGDISLPNMGLKDSNLLEEMKNQVEIIVNLAATTKF 120

Query: 121 DERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVSTGEREGIILETPFEIGESLNDTAGL 180
            ERYDVAFGTNTLGAK+VL FAKQCSNL+++VHVSTGE++G+ILETP+E+G+SL  T  L
Sbjct: 121 YERYDVAFGTNTLGAKHVLGFAKQCSNLQVLVHVSTGEKKGLILETPYEMGKSLYQTTSL 180

Query: 181 NIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEMVVGD 240
           NIETE+ +VE+T++EL+ NG TEE  TL MK LGL+RAMM+GWPNTYVFTKAMGEMVV D
Sbjct: 181 NIETERIVVEETLNELRNNGGTEERTTLAMKQLGLKRAMMHGWPNTYVFTKAMGEMVVSD 240

Query: 241 LRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTVDLIP 300
            +D++PLVIIRPTVITST K+PFPGW+E ++  D+++VGY KGKLT +P D NS +D+IP
Sbjct: 241 QKDHLPLVIIRPTVITSTCKEPFPGWIEGVRTIDSLIVGYAKGKLTSIPCDNNSILDVIP 300

Query: 301 ADMVVNAMIMAMMTHAREP-CRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMNPDGKA 360
           ADMVVN ++MA++ H  +P  + IYH+GSS RN M   D  +F +QYF+EKPW++ DG A
Sbjct: 301 ADMVVNTIVMAIVAHKLQPSSQRIYHVGSSLRNSMRYIDLKNFLFQYFIEKPWIDEDGNA 360

Query: 361 IKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSMMKLME 420
           IKV K+ VFN+M +FRR+ +IRY + LK LEFAN     S +D     ++KFD M++L E
Sbjct: 361 IKVKKIIVFNNMASFRRHMSIRYLIFLKVLEFANKASCHSLEDLYADLKRKFDWMVRLTE 420

Query: 421 LYRPYLFFKATFDDTNTERLRITARNSDLE-ETLYFDPRVVDWADYFYNVHIPAVVKYV 478
           LYRPYLFF A FDDTN E+LRI  ++ D +  TL+ DPR ++W DYF N+HIP +VK+V
Sbjct: 421 LYRPYLFFDARFDDTNLEKLRIATQDIDRDANTLFLDPRDINWEDYFVNIHIPGLVKHV 479

BLAST of MS024886 vs. ExPASy TrEMBL
Match: A0A6J1FUI8 (Fatty acyl-CoA reductase OS=Cucurbita moschata OX=3662 GN=LOC111448256 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 3.1e-172
Identity = 296/483 (61.28%), Postives = 380/483 (78.67%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           MEFL+NKNI + GA+GFL KI+VEKILR QP+VKKLYLLLR+ +E+ A +RF++EV+ +D
Sbjct: 8   MEFLENKNILITGATGFLAKILVEKILRVQPNVKKLYLLLRAANESTALERFHNEVIGKD 67

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNF 120
           LF VLK  W  + +  ISEK+C VPG+VSL  +GLKD NL+ EMK +VE+IVNLAAT  F
Sbjct: 68  LFQVLKKMWGRDFDTLISEKVCVVPGEVSLSEIGLKDSNLVAEMKNQVELIVNLAATTKF 127

Query: 121 DERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLND 180
           DERYDVA GTNTLGAK+V+SFAKQC NL+++VHVST    GER G+ILETP+++GESLN 
Sbjct: 128 DERYDVAVGTNTLGAKHVVSFAKQCPNLKLLVHVSTAYVSGERGGLILETPYKLGESLNG 187

Query: 181 TAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEM 240
             GL+I TEQK++E+ +++L+ NG+T+E ITL MK  GLERA MYGWPNTYVFTKAMGEM
Sbjct: 188 IQGLDIATEQKVMEEKLEQLEENGATKEAITLAMKDFGLERAKMYGWPNTYVFTKAMGEM 247

Query: 241 VVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTV 300
           +V DL+DN+P++IIRPT++TSTYK+PFPGW+E ++  D+++VGY KGKLT     INS V
Sbjct: 248 MVNDLKDNLPVIIIRPTIVTSTYKEPFPGWIEGIRTIDSLIVGYAKGKLTCFLAGINSIV 307

Query: 301 DLIPADMVVNAMIMAMMTHAREPC-RVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMNP 360
           DL+PADMVVN +IMAM+ H  +P  + IYH+GSS RN M   DF  F +QYF +KPW+N 
Sbjct: 308 DLVPADMVVNTIIMAMVAHELQPSDKAIYHVGSSARNSMRYIDFQRFNYQYFTKKPWINK 367

Query: 361 DGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSMM 420
           +G A+KVGK+TV+N+M +F+RY  IRY  LLKGLEFAN  F  SFQD     ++KF+ +M
Sbjct: 368 EGNAVKVGKVTVYNNMASFQRYMNIRYLCLLKGLEFANKAFCHSFQDKYVDMKRKFNLVM 427

Query: 421 KLMELYRPYLFFKATFDDTNTERLRITARNSDLE-ETLYFDPRVVDWADYFYNVHIPAVV 478
           +L++LYRPYLFF A FDDTN ERLR+     D E ET   DP+ ++W DYF NVH+P +V
Sbjct: 428 RLIQLYRPYLFFNAIFDDTNVERLRMDIEKKDREPETFLLDPKEINWEDYFMNVHLPGLV 487

BLAST of MS024886 vs. ExPASy TrEMBL
Match: A0A6J1DHV1 (Fatty acyl-CoA reductase OS=Momordica charantia OX=3673 GN=LOC111020641 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.5e-171
Identity = 303/483 (62.73%), Postives = 385/483 (79.71%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           MEFL+NK++F++GA GFLGKIMVEKILR QP+VKKLY+L+R+ DE  AA+RFY EVV +D
Sbjct: 1   MEFLENKSVFIIGALGFLGKIMVEKILRVQPNVKKLYILVRTTDEITAAQRFYYEVVGKD 60

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNF 120
           LF+ LKDKW  +LN  ISEK+  V GD+SLPN+GLKD NLL+EMK +VEIIVNLAAT  F
Sbjct: 61  LFTRLKDKWRGDLNTLISEKVRLVAGDISLPNMGLKDSNLLEEMKNQVEIIVNLAATTKF 120

Query: 121 DERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLND 180
            ERYDVAFGTNTLGAK+VL FAKQCSNL+++VHVST    GE++G+ILETP+E+G+SL  
Sbjct: 121 YERYDVAFGTNTLGAKHVLGFAKQCSNLQVLVHVSTAYVSGEKKGLILETPYEMGKSLYQ 180

Query: 181 TAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEM 240
           T  LNIETE+ +VE+T++EL+ NG TEE  TL MK LGL+RAMM+GWPNTYVFTKAMGEM
Sbjct: 181 TTSLNIETERIVVEETLNELRNNGGTEERTTLAMKQLGLKRAMMHGWPNTYVFTKAMGEM 240

Query: 241 VVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTV 300
           VV D +D++PLVIIRPTVITST K+PFPGW+E ++  D+++VGY KGKLT +P D NS +
Sbjct: 241 VVSDQKDHLPLVIIRPTVITSTCKEPFPGWIEGVRTIDSLIVGYAKGKLTSIPCDNNSIL 300

Query: 301 DLIPADMVVNAMIMAMMTHAREP-CRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMNP 360
           D+IPADMVVN ++MA++ H  +P  + IYH+GSS RN M   D  +F +QYF+EKPW++ 
Sbjct: 301 DVIPADMVVNTIVMAIVAHKLQPSSQRIYHVGSSLRNSMRYIDLKNFLFQYFIEKPWIDE 360

Query: 361 DGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSMM 420
           DG AIKV K+ VFN+M +FRR+ +IRY + LK LEFAN     S +D     ++KFD M+
Sbjct: 361 DGNAIKVKKIIVFNNMASFRRHMSIRYLIFLKVLEFANKASCHSLEDLYADLKRKFDWMV 420

Query: 421 KLMELYRPYLFFKATFDDTNTERLRITARNSDLE-ETLYFDPRVVDWADYFYNVHIPAVV 478
           +L ELYRPYLFF A FDDTN E+LRI  ++ D +  TL+ DPR ++W DYF N+HIP +V
Sbjct: 421 RLTELYRPYLFFDARFDDTNLEKLRIATQDIDRDANTLFLDPRDINWEDYFVNIHIPGLV 480

BLAST of MS024886 vs. ExPASy TrEMBL
Match: A0A1S3C9F8 (Fatty acyl-CoA reductase OS=Cucumis melo OX=3656 GN=LOC103497948 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 2.0e-171
Identity = 299/486 (61.52%), Postives = 380/486 (78.19%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           MEFL+NK+I + GA+GFL KI+VEKILR QP+VKKLYLLLR+ DE  A KRF++EVVE+ 
Sbjct: 1   MEFLENKSILITGATGFLAKILVEKILRVQPNVKKLYLLLRADDEITAKKRFHNEVVEKA 60

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNL-LKEMKEKVEIIVNLAATVN 120
           LF VLK K+  NLN  ISEKIC VPG++SLP LGLK  ++ + ++K +VEII+NLAAT N
Sbjct: 61  LFQVLKKKYGANLNTLISEKICLVPGEISLPQLGLKHDSIWIDKIKSQVEIIINLAATTN 120

Query: 121 FDERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLN 180
           FDERYDVA GTN LGAK+V++FAKQCSNL++VVHVST    GEREG+I+ETP+++GESLN
Sbjct: 121 FDERYDVALGTNALGAKHVINFAKQCSNLKLVVHVSTAYVSGEREGLIMETPYKMGESLN 180

Query: 181 DTAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGE 240
              GL+IE EQ ++E  + +LK NG+T ETITL MK LGLER+ +YGWPNTYVFTKAMGE
Sbjct: 181 WMKGLDIEAEQMVIEDKLKQLKENGATNETITLAMKDLGLERSKLYGWPNTYVFTKAMGE 240

Query: 241 MVVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINST 300
           M++GDL+D+VPL++IRPT++TSTYK+PFPGW+E ++  D+++VGY KGKLT     INS 
Sbjct: 241 MIIGDLKDDVPLIVIRPTIVTSTYKEPFPGWIEGVRTIDSLIVGYAKGKLTCFVAGINSV 300

Query: 301 VDLIPADMVVNAMIMAMMTHAREPC-RVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMN 360
           +DLIPADMVVN +I AMM H  +P   +IYH+GSSTRN +   DF  F +QYF +KP ++
Sbjct: 301 IDLIPADMVVNTIITAMMVHKLQPSNHIIYHVGSSTRNPIRHADFQRFNYQYFTKKPLID 360

Query: 361 PDGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSM 420
            DG AIKVGK+T+F  M NF RY AIRY + LKGLE  N  F  SFQD C   R+KF+ +
Sbjct: 361 RDGNAIKVGKVTIFGDMTNFHRYIAIRYLIFLKGLEIVNMAFCHSFQDKCVSMRRKFNLV 420

Query: 421 MKLMELYRPYLFFKATFDDTNTERLRITARN--SDLEETLYFDPRVVDWADYFYNVHIPA 479
           ++L++LYRPYLFF A FDDTNTERLR   +N     E  L+ DP+ ++W DYF NVHIP 
Sbjct: 421 LRLIDLYRPYLFFNAIFDDTNTERLRKELQNYKETKEGLLFMDPKDINWEDYFMNVHIPG 480

BLAST of MS024886 vs. TAIR 10
Match: AT4G33790.1 (Jojoba acyl CoA reductase-related male sterility protein )

HSP 1 Score: 515.8 bits (1327), Expect = 3.8e-146
Identity = 254/482 (52.70%), Postives = 346/482 (71.78%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           +++L NK+I V+GA+GFL  I VEKILR  P+VKKLYLLLR+     A +RF DE++++D
Sbjct: 11  LKYLDNKSILVVGAAGFLANIFVEKILRVAPNVKKLYLLLRASKGKSATQRFNDEILKKD 70

Query: 61  LFSVLKDKWNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVNF 120
           LF VLK+K+  NLN   SEKI  V GD+ L +LGL+D +L  EM  +V+ IVNLAAT  F
Sbjct: 71  LFKVLKEKYGPNLNQLTSEKITIVDGDICLEDLGLQDFDLAHEMIHQVDAIVNLAATTKF 130

Query: 121 DERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLND 180
           DERYDVA G NTLGA NVL+FAK+C+ ++I+VHVST    GE+ G+I+ETP+ +GE+LN 
Sbjct: 131 DERYDVALGINTLGALNVLNFAKRCAKVKILVHVSTAYVCGEKSGLIMETPYRMGETLNG 190

Query: 181 TAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGEM 240
           T GL+I  E+KLV++ +D+L+  G+  ETIT TMK LGL RA MYGWPNTYVFTKAMGEM
Sbjct: 191 TTGLDINYEKKLVQEKLDQLRVIGAAPETITETMKDLGLRRAKMYGWPNTYVFTKAMGEM 250

Query: 241 VVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINSTV 300
           +VG  R+N+ LV++RP++ITST+K+PFPGW E ++  D++ VGY KGKLT    D+++  
Sbjct: 251 MVGTKRENLSLVLLRPSIITSTFKEPFPGWTEGIRTIDSLAVGYGKGKLTCFLCDLDAVS 310

Query: 301 DLIPADMVVNAMIMAMMTHAREPCRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMNPD 360
           D++PADMVVN+++++M   A +   +IYH+GSS RN M    F    ++YF  KPW N +
Sbjct: 311 DVMPADMVVNSILVSMAAQAGKQEEIIYHVGSSLRNPMKNSKFPELAYRYFSIKPWTNKE 370

Query: 361 GKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSMMK 420
           GK +KVG + + +SM +F RY  IRY + LKGLE  N I    F+       KK + + +
Sbjct: 371 GKVVKVGAIEILSSMRSFHRYMTIRYLIALKGLELVNIILCKLFEKEFQYFNKKINFIFR 430

Query: 421 LMELYRPYLFFKATFDDTNTERLRITARNSDLE-ETLYFDPRVVDWADYFYNVHIPAVVK 478
           L++LY+PYLFF   FDD+NTE+LR     + +E E  YFDP+V+DW DYF N H+  ++K
Sbjct: 431 LVDLYQPYLFFYGIFDDSNTEKLRKMVSKTGVENEMFYFDPKVLDWDDYFLNTHVIGLLK 490

BLAST of MS024886 vs. TAIR 10
Match: AT5G22500.1 (fatty acid reductase 1 )

HSP 1 Score: 459.9 bits (1182), Expect = 2.5e-129
Identity = 235/486 (48.35%), Postives = 333/486 (68.52%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           ++FL NK I + GA GFL K++VEKILR QP+VKK+YLLLR+ DE  A +R   EV+E D
Sbjct: 6   VQFLGNKTILITGAPGFLAKVLVEKILRLQPNVKKIYLLLRAPDEKSAMQRLRSEVMEID 65

Query: 61  LFSVLKDKWNE-NLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVN 120
           LF VL++   E NLNA + EKI  VPGD+S+ NLGLKD +L++ M  +++II+N+AAT N
Sbjct: 66  LFKVLRNNLGEDNLNALMREKIVPVPGDISIDNLGLKDTDLIQRMWSEIDIIINIAATTN 125

Query: 121 FDERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLN 180
           FDERYD+  G NT GA NVL+FAK+C   ++++HVST    GE+ G++LE PF++GE+L+
Sbjct: 126 FDERYDIGLGINTFGALNVLNFAKKCVKGQLLLHVSTAYISGEQPGLLLEKPFKMGETLS 185

Query: 181 DTAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGE 240
               L+I  E  L+++ + EL+    ++E I+ TMK  G+ RA ++GWPNTYVFTKAMGE
Sbjct: 186 GDRELDINIEHDLMKQKLKELQ--DCSDEEISQTMKDFGMARAKLHGWPNTYVFTKAMGE 245

Query: 241 MVVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINST 300
           M++G  R+N+PLVIIRPT+ITST  +PFPGW+E +K  D+++V Y KG+L     D NS 
Sbjct: 246 MLMGKYRENLPLVIIRPTMITSTIAEPFPGWIEGLKTLDSVIVAYGKGRLKCFLADSNSV 305

Query: 301 VDLIPADMVVNAMIMAMMTHAREP-CRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMN 360
            DLIPADMVVNAM+ A   H+ +   + IYH+GSS +N +T G    FT +YF ++P + 
Sbjct: 306 FDLIPADMVVNAMVAAATAHSGDTGIQAIYHVGSSCKNPVTFGQLHDFTARYFAKRPLIG 365

Query: 361 PDGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDSM 420
            +G  I V K T+ ++M  F  Y  +RY L L+ L   N ++  S  D  +   +K    
Sbjct: 366 RNGSPIIVVKGTILSTMAQFSLYMTLRYKLPLQILRLINIVYPWSHGDNYSDLSRKIKLA 425

Query: 421 MKLMELYRPYLFFKATFDDTNTERLRITARNS--DLEETLYFDPRVVDWADYFYNVHIPA 479
           M+L+ELY+PYL FK  FDD NTERLR+  + +  +L+ +  FDP+ +DW +Y  N HIP 
Sbjct: 426 MRLVELYQPYLLFKGIFDDLNTERLRMKRKENIKELDGSFEFDPKSIDWDNYITNTHIPG 485

BLAST of MS024886 vs. TAIR 10
Match: AT3G44540.1 (fatty acid reductase 4 )

HSP 1 Score: 448.7 bits (1153), Expect = 5.7e-126
Identity = 229/491 (46.64%), Postives = 328/491 (66.80%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           ++FL +K I V G  GFL K+ VEKILR QP VKKL+LLLR+ D   A +RF+ EV+E+D
Sbjct: 6   IQFLHDKTILVTGVPGFLAKVFVEKILRIQPKVKKLFLLLRAADNESAMQRFHSEVLEKD 65

Query: 61  LFSVLKDK-WNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVN 120
           LF VLK+   +ENL AFI+EK+  +PGD+S+ NLG+K  +LL+ M  +++IIVN+AAT N
Sbjct: 66  LFRVLKNALGDENLKAFITEKVVPIPGDISVDNLGVKGSDLLQHMWNEIDIIVNVAATTN 125

Query: 121 FDERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLN 180
           FDERYDV    NT G  NVL+FAK+C   ++++HVST    GE+ G++ E  F +GE+LN
Sbjct: 126 FDERYDVGLSVNTFGPLNVLNFAKKCVKGQLLLHVSTAYVRGEKSGLLHEKTFHMGETLN 185

Query: 181 DTAGLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMGE 240
               L IETE +L+++ + EL+    +EE I+ +MK LG+ RA ++GWPNTYVFTK+MGE
Sbjct: 186 GHRKLVIETEMELMKQKLKELQKQNCSEEEISQSMKDLGMSRAKLHGWPNTYVFTKSMGE 245

Query: 241 MVVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINST 300
           M++G+ R+N+P+VIIRPT+ITST+ +PFPGW+E ++  D+++V Y KG+L     D NS 
Sbjct: 246 MLLGNYRENLPIVIIRPTMITSTFSEPFPGWIEGLRTIDSVIVAYGKGRLKCFLADPNSV 305

Query: 301 VDLIPADMVVNAMIMAMMTHA-REPCRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWMN 360
           +DLIP DMV NAM+ A   HA +   + +YH+GSS +N +T          YF + P + 
Sbjct: 306 LDLIPVDMVANAMVTAAAIHAGKLGSQTVYHVGSSCKNPITFEQIHDLAASYFTKNPLVR 365

Query: 361 PDGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIF--WGSFQDTCNKHR---K 420
            DG +I V K T+ ++M  F  Y  +RY L L+ L     I+  W       NK++   +
Sbjct: 366 RDGSSILVSKGTILSTMAQFSFYMTLRYKLPLQMLRLIYVIYPWWNG-----NKYKDIDR 425

Query: 421 KFDSMMKLMELYRPYLFFKATFDDTNTERLRITAR--NSDLEETLYFDPRVVDWADYFYN 479
           K    M+L++LYRPY+ FK  FDDTNTE+LR+  +  N ++     FDP+ +DW DY   
Sbjct: 426 KIKLAMRLVDLYRPYVLFKGIFDDTNTEKLRLKRKEINKEMYGLFEFDPKSIDWEDYMTT 485

BLAST of MS024886 vs. TAIR 10
Match: AT3G44550.1 (fatty acid reductase 5 )

HSP 1 Score: 429.9 bits (1104), Expect = 2.7e-120
Identity = 219/489 (44.79%), Postives = 320/489 (65.44%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           ++FL+NK I V GA+GFL K+ VEKILR QP+VKKLYLL+R+ D   A KR   EV E++
Sbjct: 6   VQFLRNKTILVTGATGFLAKVFVEKILRVQPNVKKLYLLVRASDNEAATKRLRTEVFEKE 65

Query: 61  LFSVLKDK-WNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVN 120
           LF VL+    +E LN  + EK+  VPGD++   LG+ D +L + M+++++I+VN+AAT N
Sbjct: 66  LFKVLRQNLGDEKLNTLLYEKVVSVPGDIATDQLGINDSHLRERMQKEIDIVVNVAATTN 125

Query: 121 FDERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLN 180
           FDERYDV  G NT GA NVL+FAK+C  +++++HVST    GE+ G+I E PF + E  N
Sbjct: 126 FDERYDVGLGINTFGALNVLNFAKKCVKVQLLLHVSTAYVCGEKPGLIPEKPFIMEEIRN 185

Query: 181 DTA-GLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMG 240
           +    L+I  E++L+++ + EL     +EE ITL+MK LG+ERA ++GWPNTYVFTK+MG
Sbjct: 186 ENGLQLDINLERELMKQRLKELNEQDCSEEDITLSMKELGMERAKLHGWPNTYVFTKSMG 245

Query: 241 EMVVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINS 300
           EM++G  ++N+PLVIIRPT+ITST  +PFPGW+E ++  D++++ Y KG L    VD+NS
Sbjct: 246 EMLLGKHKENLPLVIIRPTMITSTLSEPFPGWIEGLRTVDSVIIAYGKGVLKCFLVDVNS 305

Query: 301 TVDLIPADMVVNAMIMAMMTHA-REPCRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWM 360
             D+IP DMV NAMI A   HA      ++YH+GSS +N +T G+      +YF + P  
Sbjct: 306 VCDMIPVDMVANAMITAAAKHAGGSGVHMVYHVGSSHQNPVTFGEIHEIAVRYFTKNPLR 365

Query: 361 NPDGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDS 420
           + +G  I V K+    +M  F  Y  +RY L L+ L+  + I+     D      +K + 
Sbjct: 366 SRNGSLITVSKVRFIPTMALFSLYMTLRYKLPLQLLKLVDIIYPWRNGDKYGDKNRKIEL 425

Query: 421 MMKLMELYRPYLFFKATFDDTNTERLRITARNSDLEET----LYFDPRVVDWADYFYNVH 479
           +M+L+ELY PY+ FK  FDD NT+ L    +  +++ T      FDP+ ++W DY  N+H
Sbjct: 426 VMRLVELYEPYVLFKGIFDDRNTKSLCANQKEEEIKNTEKLMFDFDPKGINWGDYLTNIH 485

BLAST of MS024886 vs. TAIR 10
Match: AT3G44560.1 (fatty acid reductase 8 )

HSP 1 Score: 428.3 bits (1100), Expect = 8.0e-120
Identity = 217/489 (44.38%), Postives = 318/489 (65.03%), Query Frame = 0

Query: 1   MEFLKNKNIFVMGASGFLGKIMVEKILRDQPDVKKLYLLLRSVDENMAAKRFYDEVVERD 60
           + FL+NK I V GA+GFL K+ VEKILR QP+V KLYL++R+ D   A KR   E  E+D
Sbjct: 6   VHFLQNKTILVTGATGFLAKVFVEKILRVQPNVNKLYLVVRASDNEAATKRLRTEAFEKD 65

Query: 61  LFSVLKDK-WNENLNAFISEKICFVPGDVSLPNLGLKDPNLLKEMKEKVEIIVNLAATVN 120
           LF VL+D   +E LN  +SEK+  V GD+++ +LG+KD NL + M+++++I+VN+AAT N
Sbjct: 66  LFKVLRDNLGDEKLNTLLSEKVVPVAGDIAMDHLGMKDSNLRERMQKEIDIVVNVAATTN 125

Query: 121 FDERYDVAFGTNTLGAKNVLSFAKQCSNLEIVVHVST----GEREGIILETPFEIGESLN 180
           FDERYD+  G NT GA NVL+FAK+C   ++++HVST    GE+ G++ E PF + E  N
Sbjct: 126 FDERYDIGLGINTFGALNVLNFAKKCVKAQLLLHVSTAYVCGEKPGLLPEKPFVMEEICN 185

Query: 181 DTA-GLNIETEQKLVEKTIDELKTNGSTEETITLTMKHLGLERAMMYGWPNTYVFTKAMG 240
           +    L+I  E++L+++ + EL   G +EE  T  MK LG+ERA ++GWPNTYVFTK+MG
Sbjct: 186 ENGLQLDINLERELMKQRLKELNEQGCSEEGTTFYMKELGMERAKLHGWPNTYVFTKSMG 245

Query: 241 EMVVGDLRDNVPLVIIRPTVITSTYKQPFPGWVEDMKGADTIMVGYIKGKLTVLPVDINS 300
           EM++G+ ++N+PLVIIRPT+ITST  +PFPGW+E ++  D++++ Y KG L    VD+NS
Sbjct: 246 EMLLGNHKENLPLVIIRPTMITSTLFEPFPGWIEGLRTVDSVIIAYGKGVLKCFLVDVNS 305

Query: 301 TVDLIPADMVVNAMIMAMMTHA-REPCRVIYHIGSSTRNGMTCGDFLHFTWQYFVEKPWM 360
             D+IPADMV NAMI A  THA      ++Y +GSS +N +  G+     + YF +    
Sbjct: 306 VCDMIPADMVANAMIAAAATHAGGSKVHMVYQVGSSHQNPIIYGEIREILFCYFTKNSLR 365

Query: 361 NPDGKAIKVGKMTVFNSMVNFRRYTAIRYSLLLKGLEFANAIFWGSFQDTCNKHRKKFDS 420
           + +G  I V KM +  ++  F  Y  IRY L ++ L+  + I+     D      +K D 
Sbjct: 366 SRNGSMITVSKMKLIPTLALFSLYMTIRYKLPVQLLKLVDIIYPSREGDEYKNKNRKIDM 425

Query: 421 MMKLMELYRPYLFFKATFDDTNTERLRITARNSDLEET----LYFDPRVVDWADYFYNVH 479
           +M+L++LY PY+ FK  FDD NT+ L    +  D   +      FDP+++ W DY  NVH
Sbjct: 426 VMRLVKLYEPYVLFKGIFDDRNTKNLCAKQKEEDNRNSENFMFDFDPKIIKWKDYLINVH 485

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153083.12.6e-17362.47fatty acyl-CoA reductase 3-like [Momordica charantia][more]
XP_038902167.13.4e-17362.32fatty acyl-CoA reductase 3-like [Benincasa hispida][more]
XP_022153045.15.8e-17363.26fatty acyl-CoA reductase 3-like isoform X2 [Momordica charantia][more]
XP_022943504.16.4e-17261.28fatty acyl-CoA reductase 3-like [Cucurbita moschata][more]
KAG6570488.16.4e-17261.28Fatty acyl-CoA reductase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q93ZB95.3e-14552.70Fatty acyl-CoA reductase 3 OS=Arabidopsis thaliana OX=3702 GN=FAR3 PE=1 SV=1[more]
Q9XGY73.6e-13350.10Alcohol-forming fatty acyl-CoA reductase OS=Simmondsia chinensis OX=3999 PE=1 SV... [more]
Q391523.5e-12848.35Fatty acyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=FAR1 PE=1 SV=1[more]
Q9LXN38.0e-12546.64Probable fatty acyl-CoA reductase 4 OS=Arabidopsis thaliana OX=3702 GN=FAR4 PE=1... [more]
Q0WRB03.9e-11944.79Probable fatty acyl-CoA reductase 5 OS=Arabidopsis thaliana OX=3702 GN=FAR5 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1DGK41.3e-17362.47Fatty acyl-CoA reductase OS=Momordica charantia OX=3673 GN=LOC111020669 PE=3 SV=... [more]
A0A6J1DFP22.8e-17363.26Fatty acyl-CoA reductase OS=Momordica charantia OX=3673 GN=LOC111020641 PE=3 SV=... [more]
A0A6J1FUI83.1e-17261.28Fatty acyl-CoA reductase OS=Cucurbita moschata OX=3662 GN=LOC111448256 PE=3 SV=1[more]
A0A6J1DHV11.5e-17162.73Fatty acyl-CoA reductase OS=Momordica charantia OX=3673 GN=LOC111020641 PE=3 SV=... [more]
A0A1S3C9F82.0e-17161.52Fatty acyl-CoA reductase OS=Cucumis melo OX=3656 GN=LOC103497948 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G33790.13.8e-14652.70Jojoba acyl CoA reductase-related male sterility protein [more]
AT5G22500.12.5e-12948.35fatty acid reductase 1 [more]
AT3G44540.15.7e-12646.64fatty acid reductase 4 [more]
AT3G44550.12.7e-12044.79fatty acid reductase 5 [more]
AT3G44560.18.0e-12044.38fatty acid reductase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR033640Fatty acyl-CoA reductase, C-terminalPFAMPF03015Sterilecoord: 405..478
e-value: 1.7E-14
score: 53.8
IPR033640Fatty acyl-CoA reductase, C-terminalCDDcd09071FAR_Ccoord: 414..477
e-value: 7.09854E-14
score: 65.2695
NoneNo IPR availableGENE3D3.40.50.720coord: 3..366
e-value: 4.2E-68
score: 232.2
NoneNo IPR availablePANTHERPTHR11011:SF99FATTY ACYL-COA REDUCTASE 3coord: 1..477
NoneNo IPR availableCDDcd05236FAR-N_SDR_ecoord: 7..351
e-value: 1.03108E-109
score: 325.792
IPR013120Fatty acyl-coenzyme A reductase, NAD-binding domainPFAMPF07993NAD_binding_4coord: 13..308
e-value: 1.8E-70
score: 237.0
IPR026055Fatty acyl-CoA reductasePANTHERPTHR11011MALE STERILITY PROTEIN 2-RELATEDcoord: 1..477
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 2..328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS024886.1MS024886.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0080019 fatty-acyl-CoA reductase (alcohol-forming) activity