MS024502 (gene) Bitter gourd (TR) v1

Overview


Name	MS024502
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	metalloendoproteinase 2-MMP-like
Location	scaffold234: 961368 .. 962396 (-)
RNA-Seq Expression	MS024502
Synteny	MS024502

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTTCAAGGATCATTATCTTTCTTCACTTTCTCTCTTTTTCTTCTTACCCTCTTCCCTTTCATTTCTTCTCATCAAAACTCTGCGCATTCCCATGGTTTGGTGTTTCTTAACCAACTTAGCGGATACCACAAGGGCGATAAAGGGGAAGGAATTCACCAGCTAAAGGAATACCTTCACCGTTTCGGCTACTTGAACAATGTTCAGATTCATCCCGAAAATAATGACAATGAGTTTGACGAGTTCTTGGAATCTGCCATTAAAACCTACCAAATAAACTATAATCTCAAGGCCACAGGAACTATTGATGCCACGACGTTGGCTCAAATGTCAAAGCCTCGATGTGGGGTTGCAGATGTCGTTGATGGCAAGACTGGGATGAGATCTGGGAAGAAAATGAATCAACATAGAAAAATAAGTGGCCATTTCCGCACAGTTTCTCACTTCGCCTTCTTTGAAGGAAATCCTAGGTGGCCAGCTACTAAATCCCACCTCACTTACGGTAAATTTAATAGTTTATTCATATTCTAAATACTTTATGCCGGTTCCTACTCTATTCTTTGAAATTTAATTTTTTTTCCCTTATAAATAGGATTCCTCCCGGGAACCCCATCGGAGGCGATAAGTCCTGTGAGCCGAGCTTTTACGACATGGGCTGCGAACACTCACTTCACCTTCTCCCAAGCTTCTTCCTATCAAACGGCTGACATCAAAATAAGCTTTGAAAGTGGCGAGCACGGGGACGGTAATCCCTTCGATGGTGTCGGAGGAGTCATAGCCCATGCATTTTCACCCACAGATGGGAGGCTGCATTTCGACGCCGTTGAGCGTTGGGCGGTTGGCGCCGTTCCCGGTTCAAACGACGTGGAGACGGTGGCGTTGCATGAGATCGGACACCTTCTCGGCCTCCATCACAGCTCCGTTGAAGGGGCTATCATGTGGCCAACCATCATGCAAGGAGCTACCAAGGGTTTGCATGCCGATGACATTGCAGGCATAACGGCTTTGTACAATACTACTTCTTGA

mRNA sequence

ATGGCATTTCAAGGATCATTATCTTTCTTCACTTTCTCTCTTTTTCTTCTTACCCTCTTCCCTTTCATTTCTTCTCATCAAAACTCTGCGCATTCCCATGGTTTGGTGTTTCTTAACCAACTTAGCGGATACCACAAGGGCGATAAAGGGGAAGGAATTCACCAGCTAAAGGAATACCTTCACCGTTTCGGCTACTTGAACAATGTTCAGATTCATCCCGAAAATAATGACAATGAGTTTGACGAGTTCTTGGAATCTGCCATTAAAACCTACCAAATAAACTATAATCTCAAGGCCACAGGAACTATTGATGCCACGACGTTGGCTCAAATGTCAAAGCCTCGATGTGGGGTTGCAGATGTCGTTGATGGCAAGACTGGGATGAGATCTGGGAAGAAAATGAATCAACATAGAAAAATAAGTGGCCATTTCCGCACAGTTTCTCACTTCGCCTTCTTTGAAGGAAATCCTAGGTGGCCAGCTACTAAATCCCACCTCACTTACGGATTCCTCCCGGGAACCCCATCGGAGGCGATAAGTCCTGTGAGCCGAGCTTTTACGACATGGGCTGCGAACACTCACTTCACCTTCTCCCAAGCTTCTTCCTATCAAACGGCTGACATCAAAATAAGCTTTGAAAGTGGCGAGCACGGGGACGGTAATCCCTTCGATGGTGTCGGAGGAGTCATAGCCCATGCATTTTCACCCACAGATGGGAGGCTGCATTTCGACGCCGTTGAGCGTTGGGCGGTTGGCGCCGTTCCCGGTTCAAACGACGTGGAGACGGTGGCGTTGCATGAGATCGGACACCTTCTCGGCCTCCATCACAGCTCCGTTGAAGGGGCTATCATGTGGCCAACCATCATGCAAGGAGCTACCAAGGGTTTGCATGCCGATGACATTGCAGGCATAACGGCTTTGTACAATACTACTTCTTGA

Coding sequence (CDS)

ATGGCATTTCAAGGATCATTATCTTTCTTCACTTTCTCTCTTTTTCTTCTTACCCTCTTCCCTTTCATTTCTTCTCATCAAAACTCTGCGCATTCCCATGGTTTGGTGTTTCTTAACCAACTTAGCGGATACCACAAGGGCGATAAAGGGGAAGGAATTCACCAGCTAAAGGAATACCTTCACCGTTTCGGCTACTTGAACAATGTTCAGATTCATCCCGAAAATAATGACAATGAGTTTGACGAGTTCTTGGAATCTGCCATTAAAACCTACCAAATAAACTATAATCTCAAGGCCACAGGAACTATTGATGCCACGACGTTGGCTCAAATGTCAAAGCCTCGATGTGGGGTTGCAGATGTCGTTGATGGCAAGACTGGGATGAGATCTGGGAAGAAAATGAATCAACATAGAAAAATAAGTGGCCATTTCCGCACAGTTTCTCACTTCGCCTTCTTTGAAGGAAATCCTAGGTGGCCAGCTACTAAATCCCACCTCACTTACGGATTCCTCCCGGGAACCCCATCGGAGGCGATAAGTCCTGTGAGCCGAGCTTTTACGACATGGGCTGCGAACACTCACTTCACCTTCTCCCAAGCTTCTTCCTATCAAACGGCTGACATCAAAATAAGCTTTGAAAGTGGCGAGCACGGGGACGGTAATCCCTTCGATGGTGTCGGAGGAGTCATAGCCCATGCATTTTCACCCACAGATGGGAGGCTGCATTTCGACGCCGTTGAGCGTTGGGCGGTTGGCGCCGTTCCCGGTTCAAACGACGTGGAGACGGTGGCGTTGCATGAGATCGGACACCTTCTCGGCCTCCATCACAGCTCCGTTGAAGGGGCTATCATGTGGCCAACCATCATGCAAGGAGCTACCAAGGGTTTGCATGCCGATGACATTGCAGGCATAACGGCTTTGTACAATACTACTTCTTGA

Protein sequence

MAFQGSLSFFTFSLFLLTLFPFISSHQNSAHSHGLVFLNQLSGYHKGDKGEGIHQLKEYLHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGVADVVDGKTGMRSGKKMNQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATKGLHADDIAGITALYNTTS

Homology

[Close]

BLAST of MS024502 vs. NCBI nr
Match: XP_038889209.1 (metalloendoproteinase 2-MMP-like [Benincasa hispida])

HSP 1 Score: 503.1 bits (1294), Expect = 1.8e-138
Identity = 255/316 (80.70%), Postives = 282/316 (89.24%), Query Frame = 0


Query: 1   MAFQGSLSFFTFSLFLL-TLFPFISSHQ-NSAHSHGLVFLNQLSGYHKGDKGEGIHQLKE 60
           MAF+G LSFF  S FLL  +FP ISS + N+AHSHGLVFLN L G  KGDK EGIHQ+K 
Sbjct: 1   MAFEG-LSFFITSFFLLIIIFPLISSSEPNNAHSHGLVFLNNLHGCKKGDKVEGIHQMKA 60

Query: 61  YLHRFGYLNNVQIHPEN-NDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCG 120
           YL  FGYLN+VQ H ++ +D+EFDE LESAIKTYQ+NYNLKATGT+DATTLA MSKPRCG
Sbjct: 61  YLQHFGYLNHVQNHSKSFSDDEFDEVLESAIKTYQLNYNLKATGTLDATTLALMSKPRCG 120

Query: 121 VADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTPS 180
           +ADVVDGKT M+SGKKM NQHRKI+GHF +VSH+AFFEGNP+WPA+KSHLTYGFLPGTPS
Sbjct: 121 IADVVDGKTMMKSGKKMVNQHRKINGHFHSVSHYAFFEGNPKWPASKSHLTYGFLPGTPS 180

Query: 181 EAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSP 240
           EA++PV RAFTTWAANTHF+FSQAS+Y+TADIKISFESG+HGDG+ FDGVGGVIAHAFSP
Sbjct: 181 EAVAPVGRAFTTWAANTHFSFSQASAYETADIKISFESGDHGDGHAFDGVGGVIAHAFSP 240

Query: 241 TDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATKGL 300
           TDGRLHFDAVE WA+GAV  S DVETVALHEIGHLLGLHHSSVEGAIMWPTIM GATKGL
Sbjct: 241 TDGRLHFDAVEPWAIGAVADSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMGGATKGL 300

Query: 301 HADDIAGITALYNTTS 313
           HADDIAGI ALY TTS
Sbjct: 301 HADDIAGINALYTTTS 315

[Close]

BLAST of MS024502 vs. NCBI nr
Match: XP_023551500.1 (metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 497.3 bits (1279), Expect = 9.8e-137
Identity = 253/315 (80.32%), Postives = 276/315 (87.62%), Query Frame = 0


Query: 1   MAFQGSLSFFTFSLFLL-TLFPFI-SSHQNSAHSHGLVFLNQLSGYHKGDKGEGIHQLKE 60
           MA Q SLSFF  SLFLL TLFP I SSHQ  AHSHGL+FLN L+G  KGD  EG+HQ+K 
Sbjct: 1   MAVQ-SLSFFPSSLFLLITLFPLISSSHQYPAHSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGV 120
           YLHRFGYLN+VQ H +  D+EFDE LESAI+TYQ+NYNL+ATGT+DA+TLA MSKPRCGV
Sbjct: 61  YLHRFGYLNHVQNHSKTYDDEFDELLESAIQTYQLNYNLEATGTLDASTLALMSKPRCGV 120

Query: 121 ADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTPSE 180
           ADV  GKT M SGKKM N+HRKI G F  VSHFAFF+GNP+WPA+KSHLTYGFLPGTP+E
Sbjct: 121 ADVFHGKTRMNSGKKMVNRHRKIGGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTE 180

Query: 181 AISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPT 240
           AI+PV RAFTTWAANTHFTFSQASSY+TADIKISFESG+HGDG  FDGVGGVIAHAFSPT
Sbjct: 181 AIAPVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPT 240

Query: 241 DGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATKGLH 300
           DGRLHFDAVE WA+GAVP S D+ETVALHEIGHLLGL HSSVEGAIMWPTIM+GATKGLH
Sbjct: 241 DGRLHFDAVEPWAIGAVPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLH 300

Query: 301 ADDIAGITALYNTTS 313
           ADDIAGI ALY TTS
Sbjct: 301 ADDIAGINALYTTTS 314

[Close]

BLAST of MS024502 vs. NCBI nr
Match: XP_022993647.1 (metalloendoproteinase 2-MMP-like [Cucurbita maxima])

HSP 1 Score: 491.9 bits (1265), Expect = 4.1e-135
Identity = 248/315 (78.73%), Postives = 273/315 (86.67%), Query Frame = 0


Query: 1   MAFQGSLSFFTFSLFLL-TLFPFISSH-QNSAHSHGLVFLNQLSGYHKGDKGEGIHQLKE 60
           MA   +LSFF  SLFLL TLFP ISS  Q  AHSHGL+FLN L+G  KGD  EG+HQ+K 
Sbjct: 1   MAVLQALSFFASSLFLLITLFPLISSSPQYPAHSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGV 120
           YL RFGYL++VQ H +  D+EFDE LESAI+TYQ+NYNL+ATGT+DA+TLA MSKPRCGV
Sbjct: 61  YLCRFGYLSHVQNHSKTYDDEFDELLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGV 120

Query: 121 ADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTPSE 180
           ADV  GKTGM SGKKM N+HRKIS HF  VSHFAFF+GNP+WPA+KSHL YGFLPGTP E
Sbjct: 121 ADVFHGKTGMNSGKKMVNRHRKISRHFHGVSHFAFFDGNPKWPASKSHLAYGFLPGTPIE 180

Query: 181 AISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPT 240
           AI+PV RAFTTWAANTHFTFSQASSY+TADIKISFESG+HGDG  FDGVGGVIAHAFSPT
Sbjct: 181 AIAPVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPT 240

Query: 241 DGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATKGLH 300
           DGRLHFDA+E WA+GAVP S D+ETVALHEIGHLLGLHHSSVEGAIMWPTIM+GATKGLH
Sbjct: 241 DGRLHFDAIETWAIGAVPNSFDMETVALHEIGHLLGLHHSSVEGAIMWPTIMEGATKGLH 300

Query: 301 ADDIAGITALYNTTS 313
           ADDIAGI ALY  TS
Sbjct: 301 ADDIAGINALYTATS 315

[Close]

BLAST of MS024502 vs. NCBI nr
Match: KAG6578618.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 490.0 bits (1260), Expect = 1.6e-134
Identity = 247/315 (78.41%), Postives = 272/315 (86.35%), Query Frame = 0


Query: 1   MAFQGSLSFFTFSLFLL-TLFPFI-SSHQNSAHSHGLVFLNQLSGYHKGDKGEGIHQLKE 60
           MA   +LSFF+ SLFLL  LFP I SSHQ  AHSHGL+FLN L+G  KGD  EG+HQ+K 
Sbjct: 1   MAVLQALSFFSSSLFLLIALFPLISSSHQYPAHSHGLLFLNNLNGCKKGDMVEGLHQMKR 60

Query: 61  YLHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGV 120
           YL RFGYLN VQ H +  D+EFDE LESAI+TYQ+NYNL+ATGT+DA+TLA M KPRCGV
Sbjct: 61  YLRRFGYLNRVQNHSKTYDDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMLKPRCGV 120

Query: 121 ADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTPSE 180
           ADV  GKT M SGKKM N+HRKISG F  VSHFAFF+GNP+WPA+KSHLTYGFLPGTP+E
Sbjct: 121 ADVFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTE 180

Query: 181 AISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPT 240
           AI+PV RAFTTWAANTHFTFS ASSY+TADIKISFESG+HGDG  FDGVGGVIAHAFSPT
Sbjct: 181 AIAPVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPT 240

Query: 241 DGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATKGLH 300
           DGRLHFDAVE WA+GA+P S D+ETVALHEIGHLLGL HSSVEGAIMWPTIM+GATKGLH
Sbjct: 241 DGRLHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLH 300

Query: 301 ADDIAGITALYNTTS 313
           ADDIAGI ALY TTS
Sbjct: 301 ADDIAGINALYTTTS 315

[Close]

BLAST of MS024502 vs. NCBI nr
Match: KAG7016165.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 488.8 bits (1257), Expect = 3.5e-134
Identity = 247/315 (78.41%), Postives = 271/315 (86.03%), Query Frame = 0


Query: 1   MAFQGSLSFFTFSLFLL-TLFPFI-SSHQNSAHSHGLVFLNQLSGYHKGDKGEGIHQLKE 60
           MA   +LSFF  SLFLL  LFP I SSHQ  A SHGL+FLN L+G  KGD  EG+HQ+K 
Sbjct: 1   MAVLQALSFFASSLFLLIALFPLISSSHQYPAQSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGV 120
           YL RFGYLN VQ H +  D+EFDE LESAI+TYQ+NYNL+ATGT+DA+TLA MSKPRCGV
Sbjct: 61  YLRRFGYLNRVQNHSKTYDDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGV 120

Query: 121 ADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTPSE 180
           ADV  GKT M SGKKM N+HRKISG F  VSHFAFF+GNP+WPA+KSHLTYGFLPGTP+E
Sbjct: 121 ADVFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTE 180

Query: 181 AISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPT 240
           AI+PV RAFTTWAANTHFTFS ASSY+TADIKISFESG+HGDG  FDGVGGVIAHAFSPT
Sbjct: 181 AIAPVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPT 240

Query: 241 DGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATKGLH 300
           DGRLHFDAVE WA+GA+P S D+ETVALHEIGHLLGL HSSVEGAIMWPTIM+GATKGLH
Sbjct: 241 DGRLHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLH 300

Query: 301 ADDIAGITALYNTTS 313
           ADDIAGI ALY TTS
Sbjct: 301 ADDIAGINALYTTTS 315

[Close]

BLAST of MS024502 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.0e-59
Identity = 127/285 (44.56%), Postives = 163/285 (57.19%), Query Frame = 0


Query: 37  FLNQLSGYHKGDKGEGIHQLKEYLHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYN 96
           FLN  +G H G K +G++ LK+Y   FGY+    +   N  ++FD+ L++A++ YQ N+ 
Sbjct: 46  FLN-FTGCHAGKKYDGLYMLKQYFQHFGYITETNL-SGNFTDDFDDILKNAVEMYQRNFQ 105

Query: 97  LKATGTIDATTLAQMSKPRCGVADVVDGKTGMRSGKKMNQ--HRKISGHFRTVSHFAFFE 156
           L  TG +D  TL  +  PRCG  DVV+G + M SG+K  +         F  V H++FF 
Sbjct: 106 LNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFP 165

Query: 157 GNPRWPATKSHLTYGFLP--GTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISF 216
           G PRWP  +  LTY F P      E  S  SRAFT W   T  TF++   + T+DI I F
Sbjct: 166 GEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGF 225

Query: 217 ESGEHGDGNPFDGVGGVIAHAFSPTDGRLHFDAVERWAVGAVPGSN--------DVETVA 276
            SGEHGDG PFDG    +AHAFSP  G  H D  E W V    G          D+E+VA
Sbjct: 226 YSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVA 285

Query: 277 LHEIGHLLGLHHSSVEGAIMWPTIMQGATK-GLHADDIAGITALY 309
           +HEIGHLLGL HSSVEG+IM+PTI  G  K  L  DD+ G+  LY
Sbjct: 286 VHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of MS024502 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-57
Identity = 140/321 (43.61%), Postives = 176/321 (54.83%), Query Frame = 0


Query: 11  TFSLFLLTLFPFISS-HQNSAHSHGLVFLN----------QLSGYHKGDKGEGIHQLKEY 70
           T  +F  T+ P  +  + N +    L FLN          +L+G H G+   G+ +LK+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 71  LHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGVA 130
             RFGY+        N  ++FD+ L+SAI TYQ N+NLK TG +D++TL Q+ KPRCG  
Sbjct: 67  FRRFGYITTT----GNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGNP 126

Query: 131 DVVDGKTGMRSGKKMNQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLP--GTPSE 190
           D++DG + M  GK +          RT   ++FF G PRWP  K  LTY F P      E
Sbjct: 127 DLIDGVSEMNGGKIL----------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDE 186

Query: 191 AISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPT 250
                SRAFT WA  T   F+++ S   ADI I F SGEHGDG PFDG  G +AHA SP 
Sbjct: 187 VKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPP 246

Query: 251 DGRLHFDAVERWAVG---------AVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTI 309
            G LH D  E W +           V    D+E+VA+HEIGHLLGL HSSVE AIM+P I
Sbjct: 247 TGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAI 306

[Close]

BLAST of MS024502 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 4.7e-57
Identity = 132/320 (41.25%), Postives = 180/320 (56.25%), Query Frame = 0


Query: 9   FFTFSLFLLT------LFPFISSHQNSAHSHGLVF---LNQLSGYHKGDKGEGIHQLKEY 68
           F   SLFL+        FP  ++   S  +   VF    +  +G H G   +G++++K+Y
Sbjct: 6   FGFLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKY 65

Query: 69  LHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGVA 128
             RFGY+   +    N  ++FD+ L++A++ YQ N+NL  TG +DA T+  +  PRCG  
Sbjct: 66  FQRFGYI--PETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNP 125

Query: 129 DVVDGKTGMRSGKKMNQHRKIS-GHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTP--S 188
           DVV+G + M  G++       S  H   V  +  F G PRWP  +  LTY F P  P   
Sbjct: 126 DVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTE 185

Query: 189 EAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSP 248
           E  S  SRAF  W+  T   F+ + S+ T+DI I F +G+HGDG PFDGV G +AHAFSP
Sbjct: 186 EVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSP 245

Query: 249 TDGRLHFDAVERWAVG-------AVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 308
             G+ H DA E W V        +V  + D+E+VA+HEIGHLLGL HSSVE +IM+PTI 
Sbjct: 246 PSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTIT 305

[Close]

BLAST of MS024502 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 216.1 bits (549), Expect = 5.7e-55
Identity = 130/303 (42.90%), Postives = 169/303 (55.78%), Query Frame = 0


Query: 15  FLLTLFPFISSHQNSAHSHGLVFLNQLSGYHKGDKGEGIHQLKEYLHRFGYLNNVQIHPE 74
           FL T  P +S+H   A      +  + + YH G   +G+  +K Y H  GY+ N    P 
Sbjct: 18  FLATSLPSVSAHGPYAWDGEATY--KFTTYHPGQNYKGLSNVKNYFHHLGYIPNA---PH 77

Query: 75  NNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGVADVVDGKTGMRSGKKM 134
            +DN FD+ L SAIKTYQ NYNL  TG  D  TL Q+  PRCGV D++           +
Sbjct: 78  FDDN-FDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVPDII-----------I 137

Query: 135 NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTPSEAI--SPVSRAFTTWAAN 194
           N ++  S  F  +S + FF+  PRW A  + LTY F P    +    S ++RAF+ W   
Sbjct: 138 NTNKTTS--FGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDDTFKSAIARAFSKWTPV 197

Query: 195 THFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPTDGRLHFDAVERWAVG 254
            +  F + +SY+TA+IKI F S  HGD  PFDG GG++ HAF+PTDGR HFDA E W   
Sbjct: 198 VNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDGRCHFDADEYWVAS 257

Query: 255 A------VPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATK-GLHADDIAGIT 309
                  V  + D+E+VA+HEIGHLLGL HSS   AIM+P+I     K  L  DDI GI 
Sbjct: 258 GDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDIDGIR 301

[Close]

BLAST of MS024502 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.0e-48
Identity = 117/275 (42.55%), Postives = 155/275 (56.36%), Query Frame = 0


Query: 47  GDKGEGIHQLKEYLHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDAT 106
           G    G+ +LK YLHRFGY+N+     E   + FD  LESAI  YQ N  L  TG +D +
Sbjct: 64  GSHVSGVSELKRYLHRFGYVND---GSEIFSDVFDGPLESAISLYQENLGLPITGRLDTS 123

Query: 107 TLAQMSKPRCGVADVVDGKTGMRSGKKMNQHRKISGHF-RTVSHFAFFEGNPRWPATKSH 166
           T+  MS PRCGV+D                H  I+  F  T +H+ +F G P+W   +  
Sbjct: 124 TVTLMSLPRCGVSDT---------------HMTINNDFLHTTAHYTYFNGKPKW--NRDT 183

Query: 167 LTYGF-----LPGTPSEAISPV-SRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGD 226
           LTY       L    SE +  V  RAF+ W++    +F +   + TAD+KI F +G+HGD
Sbjct: 184 LTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGD 243

Query: 227 GNPFDGVGGVIAHAFSPTDGRLHFDAVERWAV-----GAVPGSNDVETVALHEIGHLLGL 286
           G PFDGV G +AHAF+P +GRLH DA E W V     G+   + D+E+VA HEIGHLLGL
Sbjct: 244 GLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGL 303

Query: 287 HHSSVEGAIMWPTIMQGATK-GLHADDIAGITALY 309
            HSS E A+M+P++     K  L  DD+AG+  LY
Sbjct: 304 GHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of MS024502 vs. ExPASy TrEMBL
Match: A0A6J1JTF5 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=3 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 2.0e-135
Identity = 248/315 (78.73%), Postives = 273/315 (86.67%), Query Frame = 0


Query: 1   MAFQGSLSFFTFSLFLL-TLFPFISSH-QNSAHSHGLVFLNQLSGYHKGDKGEGIHQLKE 60
           MA   +LSFF  SLFLL TLFP ISS  Q  AHSHGL+FLN L+G  KGD  EG+HQ+K 
Sbjct: 1   MAVLQALSFFASSLFLLITLFPLISSSPQYPAHSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGV 120
           YL RFGYL++VQ H +  D+EFDE LESAI+TYQ+NYNL+ATGT+DA+TLA MSKPRCGV
Sbjct: 61  YLCRFGYLSHVQNHSKTYDDEFDELLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGV 120

Query: 121 ADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTPSE 180
           ADV  GKTGM SGKKM N+HRKIS HF  VSHFAFF+GNP+WPA+KSHL YGFLPGTP E
Sbjct: 121 ADVFHGKTGMNSGKKMVNRHRKISRHFHGVSHFAFFDGNPKWPASKSHLAYGFLPGTPIE 180

Query: 181 AISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPT 240
           AI+PV RAFTTWAANTHFTFSQASSY+TADIKISFESG+HGDG  FDGVGGVIAHAFSPT
Sbjct: 181 AIAPVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPT 240

Query: 241 DGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATKGLH 300
           DGRLHFDA+E WA+GAVP S D+ETVALHEIGHLLGLHHSSVEGAIMWPTIM+GATKGLH
Sbjct: 241 DGRLHFDAIETWAIGAVPNSFDMETVALHEIGHLLGLHHSSVEGAIMWPTIMEGATKGLH 300

Query: 301 ADDIAGITALYNTTS 313
           ADDIAGI ALY  TS
Sbjct: 301 ADDIAGINALYTATS 315

[Close]

BLAST of MS024502 vs. ExPASy TrEMBL
Match: A0A6J1FDT9 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 8.4e-134
Identity = 246/315 (78.10%), Postives = 270/315 (85.71%), Query Frame = 0


Query: 1   MAFQGSLSFFTFSLFLL-TLFPFI-SSHQNSAHSHGLVFLNQLSGYHKGDKGEGIHQLKE 60
           MA   +LSF   SLFLL  LFP I SSHQ  A SHGL+FLN L+G  KGD  EG+HQ+K 
Sbjct: 1   MAVLQALSFLASSLFLLIALFPLISSSHQYPAQSHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGV 120
           YL RFGYLN VQ H +  D+EFDE LESAI+TYQ+NYNL+ATGT+DA+TLA MSKPRCGV
Sbjct: 61  YLRRFGYLNRVQNHSKTYDDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGV 120

Query: 121 ADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTPSE 180
           ADV  GKT M SGKKM N+HRKISG F  VSHFAFF+GNP+WPA+KSHLTYGFLPGTP+E
Sbjct: 121 ADVFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTE 180

Query: 181 AISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPT 240
           AI+PV RAFTTWAANTHFTFS ASSY+TADIKISFESG+HGDG  FDGVGGVIAHAFSPT
Sbjct: 181 AIAPVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPT 240

Query: 241 DGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATKGLH 300
           DGRLHFDAVE WA+GA+P S D+ETVALHEIGHLLGL HSSVEGAIMWPTIM+GATKGLH
Sbjct: 241 DGRLHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLH 300

Query: 301 ADDIAGITALYNTTS 313
           ADDIAGI ALY TTS
Sbjct: 301 ADDIAGINALYTTTS 315

[Close]

BLAST of MS024502 vs. ExPASy TrEMBL
Match: A0A0A0KS47 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 6.8e-128
Identity = 236/314 (75.16%), Postives = 263/314 (83.76%), Query Frame = 0


Query: 1   MAFQGSLSFFTFSLFL-LTLFPFISSHQNSAHSHGLVFLNQLSGYHKGDKGEGIHQLKEY 60
           M FQG   FFTF+LFL ++LFP ISS Q      GLVFL  L G  KGDK EGIHQ+K+Y
Sbjct: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKP-VDGLVFLKNLKGCKKGDKVEGIHQMKKY 60

Query: 61  LHRFGYLNN-VQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGV 120
           L  FGYLNN V I  E++D+EFDE LE A+KTYQINYNLK TGT+D +TL QMSKPRCGV
Sbjct: 61  LQHFGYLNNDVHIRSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGV 120

Query: 121 ADVVDGKTGMRSGKK-MNQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTPSE 180
           AD+++GKT M+SGK+ +NQHRKISGHF  VSHFAFFEGN RWP +KSHLTYGFLPGTPSE
Sbjct: 121 ADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTPSE 180

Query: 181 AISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPT 240
           AISPV+RAFTTWAANTHF+FSQ S Y+ ADIKISFE G+HGDG PFD VGGV+AHAF+PT
Sbjct: 181 AISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAPT 240

Query: 241 DGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATKGLH 300
           DGRLHFDAVE WA GAVP S D+ETVALHEIGHLLGLHHSSVEGAIMWP+IM GATKGLH
Sbjct: 241 DGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKGLH 300

Query: 301 ADDIAGITALYNTT 312
           ADDI GI  LY T+
Sbjct: 301 ADDIEGIKVLYTTS 313

[Close]

BLAST of MS024502 vs. ExPASy TrEMBL
Match: A0A5D3BMC5 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00180 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 5.4e-125
Identity = 230/314 (73.25%), Postives = 264/314 (84.08%), Query Frame = 0


Query: 1   MAFQGSLSFFTFSLFL-LTLFPFI-SSHQNSAHSHGLVFLNQLSGYHKGDKGEGIHQLKE 60
           M F+  LSF T S FL + LFP I SSH N  HS+GL FL  L+G  KGDK  GIHQ+KE
Sbjct: 1   MTFEKLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLHRFGYLNN-VQIHPEN-NDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRC 120
           YL  FGYLNN +Q + ++ +D+EFD  LESAIKTYQ+NYNLKATGT+DA TL  MSKPRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GVADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTP 180
           GVAD++DGKT M+SGKKM NQHRKI+GHF TVSH+AFF+GNP+WPA+KSHLTYGFLPGTP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFS 240
           S+A++PV RAF TW A+THF FS+AS Y+ ADIKISF SG+HGDG+ FDGVGGVIAH F+
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATKG 300
           PTDGRLHFDA+E WAVGAV  S D+ETVALHEIGHLLGLHHSSVEGAIMWP+IM+G TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LHADDIAGITALYN 310
           L ADDIAGI ALYN
Sbjct: 301 LDADDIAGINALYN 314

[Close]

BLAST of MS024502 vs. ExPASy TrEMBL
Match: A0A1S3C9I6 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.7e-124
Identity = 229/314 (72.93%), Postives = 263/314 (83.76%), Query Frame = 0


Query: 1   MAFQGSLSFFTFSLFL-LTLFPFI-SSHQNSAHSHGLVFLNQLSGYHKGDKGEGIHQLKE 60
           M F+  LSF T S FL + LFP I SSH N  HS+GL FL  L+G  KGDK  GIHQ+KE
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLHRFGYLNN-VQIHPEN-NDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRC 120
           YL  FGYLNN +Q + ++ +D+EFD  LESAIKTYQ+NYNLKATGT+DA TL  MSKPRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GVADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTP 180
           GVAD++DGKT M+SGKKM NQHRKI+GHF TVSH+AFF+GNP+WPA+K HLTYGFLPGTP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYGFLPGTP 180

Query: 181 SEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFS 240
           S+A++PV RAF TW A+THF FS+AS Y+ ADIKISF SG+HGDG+ FDGVGGVIAH F+
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATKG 300
           PTDGRLHFDA+E WAVGAV  S D+ETVALHEIGHLLGLHHSSVEGAIMWP+IM+G TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LHADDIAGITALYN 310
           L ADDIAGI ALYN
Sbjct: 301 LDADDIAGINALYN 314

[Close]

BLAST of MS024502 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 229.6 bits (584), Expect = 3.5e-60
Identity = 127/285 (44.56%), Postives = 163/285 (57.19%), Query Frame = 0


Query: 37  FLNQLSGYHKGDKGEGIHQLKEYLHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYN 96
           FLN  +G H G K +G++ LK+Y   FGY+    +   N  ++FD+ L++A++ YQ N+ 
Sbjct: 46  FLN-FTGCHAGKKYDGLYMLKQYFQHFGYITETNL-SGNFTDDFDDILKNAVEMYQRNFQ 105

Query: 97  LKATGTIDATTLAQMSKPRCGVADVVDGKTGMRSGKKMNQ--HRKISGHFRTVSHFAFFE 156
           L  TG +D  TL  +  PRCG  DVV+G + M SG+K  +         F  V H++FF 
Sbjct: 106 LNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFP 165

Query: 157 GNPRWPATKSHLTYGFLP--GTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISF 216
           G PRWP  +  LTY F P      E  S  SRAFT W   T  TF++   + T+DI I F
Sbjct: 166 GEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGF 225

Query: 217 ESGEHGDGNPFDGVGGVIAHAFSPTDGRLHFDAVERWAVGAVPGSN--------DVETVA 276
            SGEHGDG PFDG    +AHAFSP  G  H D  E W V    G          D+E+VA
Sbjct: 226 YSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVA 285

Query: 277 LHEIGHLLGLHHSSVEGAIMWPTIMQGATK-GLHADDIAGITALY 309
           +HEIGHLLGL HSSVEG+IM+PTI  G  K  L  DD+ G+  LY
Sbjct: 286 VHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of MS024502 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 224.9 bits (572), Expect = 8.7e-59
Identity = 140/321 (43.61%), Postives = 176/321 (54.83%), Query Frame = 0


Query: 11  TFSLFLLTLFPFISS-HQNSAHSHGLVFLN----------QLSGYHKGDKGEGIHQLKEY 70
           T  +F  T+ P  +  + N +    L FLN          +L+G H G+   G+ +LK+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 71  LHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGVA 130
             RFGY+        N  ++FD+ L+SAI TYQ N+NLK TG +D++TL Q+ KPRCG  
Sbjct: 67  FRRFGYITTT----GNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGNP 126

Query: 131 DVVDGKTGMRSGKKMNQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLP--GTPSE 190
           D++DG + M  GK +          RT   ++FF G PRWP  K  LTY F P      E
Sbjct: 127 DLIDGVSEMNGGKIL----------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDE 186

Query: 191 AISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPT 250
                SRAFT WA  T   F+++ S   ADI I F SGEHGDG PFDG  G +AHA SP 
Sbjct: 187 VKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPP 246

Query: 251 DGRLHFDAVERWAVG---------AVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTI 309
            G LH D  E W +           V    D+E+VA+HEIGHLLGL HSSVE AIM+P I
Sbjct: 247 TGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAI 306

[Close]

BLAST of MS024502 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 223.0 bits (567), Expect = 3.3e-58
Identity = 132/320 (41.25%), Postives = 180/320 (56.25%), Query Frame = 0


Query: 9   FFTFSLFLLT------LFPFISSHQNSAHSHGLVF---LNQLSGYHKGDKGEGIHQLKEY 68
           F   SLFL+        FP  ++   S  +   VF    +  +G H G   +G++++K+Y
Sbjct: 6   FGFLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKY 65

Query: 69  LHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGVA 128
             RFGY+   +    N  ++FD+ L++A++ YQ N+NL  TG +DA T+  +  PRCG  
Sbjct: 66  FQRFGYI--PETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNP 125

Query: 129 DVVDGKTGMRSGKKMNQHRKIS-GHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTP--S 188
           DVV+G + M  G++       S  H   V  +  F G PRWP  +  LTY F P  P   
Sbjct: 126 DVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTE 185

Query: 189 EAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSP 248
           E  S  SRAF  W+  T   F+ + S+ T+DI I F +G+HGDG PFDGV G +AHAFSP
Sbjct: 186 EVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSP 245

Query: 249 TDGRLHFDAVERWAVG-------AVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 308
             G+ H DA E W V        +V  + D+E+VA+HEIGHLLGL HSSVE +IM+PTI 
Sbjct: 246 PSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTIT 305

[Close]

BLAST of MS024502 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 195.3 bits (495), Expect = 7.4e-50
Identity = 117/275 (42.55%), Postives = 155/275 (56.36%), Query Frame = 0


Query: 47  GDKGEGIHQLKEYLHRFGYLNNVQIHPENNDNEFDEFLESAIKTYQINYNLKATGTIDAT 106
           G    G+ +LK YLHRFGY+N+     E   + FD  LESAI  YQ N  L  TG +D +
Sbjct: 64  GSHVSGVSELKRYLHRFGYVND---GSEIFSDVFDGPLESAISLYQENLGLPITGRLDTS 123

Query: 107 TLAQMSKPRCGVADVVDGKTGMRSGKKMNQHRKISGHF-RTVSHFAFFEGNPRWPATKSH 166
           T+  MS PRCGV+D                H  I+  F  T +H+ +F G P+W   +  
Sbjct: 124 TVTLMSLPRCGVSDT---------------HMTINNDFLHTTAHYTYFNGKPKW--NRDT 183

Query: 167 LTYGF-----LPGTPSEAISPV-SRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGD 226
           LTY       L    SE +  V  RAF+ W++    +F +   + TAD+KI F +G+HGD
Sbjct: 184 LTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGD 243

Query: 227 GNPFDGVGGVIAHAFSPTDGRLHFDAVERWAV-----GAVPGSNDVETVALHEIGHLLGL 286
           G PFDGV G +AHAF+P +GRLH DA E W V     G+   + D+E+VA HEIGHLLGL
Sbjct: 244 GLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGL 303

Query: 287 HHSSVEGAIMWPTIMQGATK-GLHADDIAGITALY 309
            HSS E A+M+P++     K  L  DD+AG+  LY
Sbjct: 304 GHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of MS024502 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 176.8 bits (447), Expect = 2.7e-44
Identity = 114/306 (37.25%), Postives = 160/306 (52.29%), Query Frame = 0


Query: 18  TLFPFISSHQNSAHSHGLVFLNQLSGYHKGDKGE-GIHQLKEYLHRFGYLNNVQIHPENN 77
           T+F F   + N  H+  ++     S +      +  I ++K +L ++GYL      P+N 
Sbjct: 15  TIFSFFLLYLN-LHNQQIIEARNPSQFTTNPSPDVSIPEIKRHLQQYGYL------PQNK 74

Query: 78  DNEFDEFLESAIKTYQINYNLKATGTIDATTLAQMSKPRCGVADVVDGKTGMRSGKKMNQ 137
           +++ D   E A+  YQ N  L  TG  D+ TL+Q+  PRCG  D V+ KT          
Sbjct: 75  ESD-DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRCGFPDDVEPKT---------- 134

Query: 138 HRKISGHFRTVSHFAFFEGNPRWPA-TKSHLTYGFLPGTPSEAISPVS------RAFTTW 197
                  F T   + +F G PRW       LTY F     +  ++P        RAF  W
Sbjct: 135 -----APFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRRAFGKW 194

Query: 198 AANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPTDGRLHFDAVERW 257
           A+    +F +   Y  ADIKI F +G+HGDG PFDGV GV+AH FSP +GRLH D  E W
Sbjct: 195 ASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETW 254

Query: 258 AVGAVPGSN----DVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATK-GLHADDIAGI 311
           AV      +    D+E+VA+HEIGH+LGL HSSV+ A M+PT+   + K  L+ DD+ G+
Sbjct: 255 AVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGV 297

The following BLAST results are available for this feature:

BLAST of MS024502 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889209.1	1.8e-138	80.70	metalloendoproteinase 2-MMP-like [Benincasa hispida]	[more]
XP_023551500.1	9.8e-137	80.32	metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo]	[more]
XP_022993647.1	4.1e-135	78.73	metalloendoproteinase 2-MMP-like [Cucurbita maxima]	[more]
KAG6578618.1	1.6e-134	78.41	Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7016165.1	3.5e-134	78.41	Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]

BLAST of MS024502 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5XF51	5.0e-59	44.56	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
Q9ZUJ5	1.2e-57	43.61	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
O04529	4.7e-57	41.25	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
P29136	5.7e-55	42.90	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
O23507	1.0e-48	42.55	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]

BLAST of MS024502 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JTF5	2.0e-135	78.73	metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=...	[more]
A0A6J1FDT9	8.4e-134	78.10	metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 P...	[more]
A0A0A0KS47	6.8e-128	75.16	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 S...	[more]
A0A5D3BMC5	5.4e-125	73.25	Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C9I6	2.7e-124	72.93	metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV...	[more]

BLAST of MS024502 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24140.1	3.5e-60	44.56	Matrixin family protein	[more]
AT1G59970.1	8.7e-59	43.61	Matrixin family protein	[more]
AT1G70170.1	3.3e-58	41.25	matrix metalloproteinase	[more]
AT4G16640.1	7.4e-50	42.55	Matrixin family protein	[more]
AT2G45040.1	2.7e-44	37.25	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 111..124 score: 55.4 coord: 206..234 score: 62.03 coord: 182..197 score: 34.91 coord: 263..288 score: 57.69 coord: 296..309 score: 53.22
IPR021190	Peptidase M10A	PIRSF	PIRSF001191	MMP_stromelysin	coord: 20..312 e-value: 1.3E-60 score: 203.3
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 155..310 e-value: 5.2E-30 score: 115.7
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 50..111 e-value: 1.2E-13 score: 51.0
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 162..308 e-value: 1.8E-43 score: 148.1
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 44..312 e-value: 4.9E-77 score: 260.7
None	No IPR available	PANTHER	PTHR10201:SF269	METALLOENDOPROTEINASE 3-MMP-LIKE	coord: 12..309
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 12..309
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 152..309
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 158..308 e-value: 6.36836E-58 score: 181.633
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 43..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS024502.1	MS024502.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0008270	zinc ion binding