MS024497 (gene) Bitter gourd (TR) v1

Overview


Name	MS024497
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	metalloendoproteinase 3-MMP-like
Location	scaffold234: 895941 .. 896402 (-)
RNA-Seq Expression	MS024497
Synteny	MS024497

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCCTTCAAAATACAGCTTGACCTACACTTTCCTTCGGGTTTCCCACAGGACAAAGTAGGTGCGGTGGTTGCAGCTTTCAACGAGTGGGATTCCAAAACCAAGTTCAGCTTCACACGTGTGGCCAACAATGTCCGAGCTGACATCACAATAAGCTTCCAAAGAGGGAACCATGGAGATGGCTATCCGTTTGATGGGCCTGGGGGAGTGCTGGCACATGCCTTTGCGCCAACCGACGGCAGGCTTCACTTAGACGGCGATGATCGGTATGCGGTGGGCGCTGTTCCTGGCAATTTTGACACCTCGAGCGTGGTGGTGCATGAGATTGGCCACATTATGGGCCTCGGCCACAGCAACGATCCTCAGGCCATTATGTACCCATACATTAACGATGGCGTTGTCAAGGCCACCTTGCAGCAAGACGATCTTAATGGAATTCGGGATTTATATGGACCTTGA

mRNA sequence

ATGGCCTCCTTCAAAATACAGCTTGACCTACACTTTCCTTCGGGTTTCCCACAGGACAAAGTAGGTGCGGTGGTTGCAGCTTTCAACGAGTGGGATTCCAAAACCAAGTTCAGCTTCACACGTGTGGCCAACAATGTCCGAGCTGACATCACAATAAGCTTCCAAAGAGGGAACCATGGAGATGGCTATCCGTTTGATGGGCCTGGGGGAGTGCTGGCACATGCCTTTGCGCCAACCGACGGCAGGCTTCACTTAGACGGCGATGATCGGTATGCGGTGGGCGCTGTTCCTGGCAATTTTGACACCTCGAGCGTGGTGGTGCATGAGATTGGCCACATTATGGGCCTCGGCCACAGCAACGATCCTCAGGCCATTATGTACCCATACATTAACGATGGCGTTGTCAAGGCCACCTTGCAGCAAGACGATCTTAATGGAATTCGGGATTTATATGGACCTTGA

Coding sequence (CDS)

ATGGCCTCCTTCAAAATACAGCTTGACCTACACTTTCCTTCGGGTTTCCCACAGGACAAAGTAGGTGCGGTGGTTGCAGCTTTCAACGAGTGGGATTCCAAAACCAAGTTCAGCTTCACACGTGTGGCCAACAATGTCCGAGCTGACATCACAATAAGCTTCCAAAGAGGGAACCATGGAGATGGCTATCCGTTTGATGGGCCTGGGGGAGTGCTGGCACATGCCTTTGCGCCAACCGACGGCAGGCTTCACTTAGACGGCGATGATCGGTATGCGGTGGGCGCTGTTCCTGGCAATTTTGACACCTCGAGCGTGGTGGTGCATGAGATTGGCCACATTATGGGCCTCGGCCACAGCAACGATCCTCAGGCCATTATGTACCCATACATTAACGATGGCGTTGTCAAGGCCACCTTGCAGCAAGACGATCTTAATGGAATTCGGGATTTATATGGACCTTGA

Protein sequence

MASFKIQLDLHFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYPFDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQAIMYPYINDGVVKATLQQDDLNGIRDLYGP

Homology

[Close]

BLAST of MS024497 vs. NCBI nr
Match: XP_022134258.1 (metalloendoproteinase 3-MMP-like [Momordica charantia])

HSP 1 Score: 303.5 bits (776), Expect = 1.0e-78
Identity = 143/149 (95.97%), Postives = 144/149 (96.64%), Query Frame = 0


Query: 5   KIQLDLHFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP 64
           K  L  +FPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP
Sbjct: 65  KYSLTYNFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP 124

Query: 65  FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA 124
           FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA
Sbjct: 125 FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA 184

Query: 125 IMYPYINDGVVKATLQQDDLNGIRDLYGP 154
           IMYPYINDGVVKATLQQDDLNGIR LYGP
Sbjct: 185 IMYPYINDGVVKATLQQDDLNGIRALYGP 213

[Close]

BLAST of MS024497 vs. NCBI nr
Match: XP_022959874.1 (metalloendoproteinase 1-like [Cucurbita moschata])

HSP 1 Score: 190.3 bits (482), Expect = 1.3e-44
Identity = 89/148 (60.14%), Postives = 105/148 (70.95%), Query Frame = 0


Query: 5   KIQLDLHFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP 64
           K  L   F +GFP +    V +A   W + T+F+FT V ++  ADITI+F R +HGDGYP
Sbjct: 63  KRNLTYKFLTGFPTNGKPPVASACGRWAAVTQFTFTEVPSSAPADITITFARLSHGDGYP 122

Query: 65  FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA 124
           FDGPGG LAHAFAPTDGRLH D DD Y +GA P  FD  SV VHE+GHI+GLGHSN  QA
Sbjct: 123 FDGPGGTLAHAFAPTDGRLHFDADDSYVIGAAPNAFDLESVAVHEMGHILGLGHSNVVQA 182

Query: 125 IMYPYINDGVVKATLQQDDLNGIRDLYG 153
           IMYP I+ G VK  LQ DD+NGI+ LYG
Sbjct: 183 IMYPSISSGTVKKELQADDINGIKALYG 210

[Close]

BLAST of MS024497 vs. NCBI nr
Match: KAG6602460.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 190.3 bits (482), Expect = 1.3e-44
Identity = 89/148 (60.14%), Postives = 105/148 (70.95%), Query Frame = 0


Query: 5   KIQLDLHFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP 64
           K  L   F +GFP +    V +A   W + T+F+FT V ++  ADITI+F R +HGDGYP
Sbjct: 63  KRNLTYKFLTGFPTNGKPPVASACGRWAAVTQFTFTEVPSSAPADITITFARLSHGDGYP 122

Query: 65  FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA 124
           FDGPGG LAHAFAPTDGRLH D DD Y +GA P  FD  SV VHE+GHI+GLGHSN  QA
Sbjct: 123 FDGPGGTLAHAFAPTDGRLHFDADDSYVIGAAPNAFDLESVAVHEMGHILGLGHSNVVQA 182

Query: 125 IMYPYINDGVVKATLQQDDLNGIRDLYG 153
           IMYP I+ G VK  LQ DD+NGI+ LYG
Sbjct: 183 IMYPSISSGTVKKELQADDINGIKALYG 210

[Close]

BLAST of MS024497 vs. NCBI nr
Match: XP_022991022.1 (metalloendoproteinase 2-MMP-like [Cucurbita maxima] >XP_022991023.1 metalloendoproteinase 2-MMP-like [Cucurbita maxima])

HSP 1 Score: 189.1 bits (479), Expect = 2.8e-44
Identity = 89/148 (60.14%), Postives = 104/148 (70.27%), Query Frame = 0


Query: 5   KIQLDLHFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP 64
           K  L+  F +GFP +    V +A   W + T F+FT V ++  ADITI+F R NHGDGY 
Sbjct: 63  KRNLNYKFLTGFPTNGKPPVASACGRWAAVTPFTFTEVPSSAPADITITFARLNHGDGYS 122

Query: 65  FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA 124
           FDG GG LAHAFAPTDGRLH D DD Y +GA PG FD  SV VHE+GHI+GLGHS  PQA
Sbjct: 123 FDGKGGTLAHAFAPTDGRLHFDADDPYVIGAAPGAFDLESVAVHEMGHILGLGHSTVPQA 182

Query: 125 IMYPYINDGVVKATLQQDDLNGIRDLYG 153
           IMYP I+ G VK  LQ DD+NGI+ LYG
Sbjct: 183 IMYPSISSGTVKKDLQADDINGIKALYG 210

[Close]

BLAST of MS024497 vs. NCBI nr
Match: XP_023544550.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 187.2 bits (474), Expect = 1.1e-43
Identity = 89/148 (60.14%), Postives = 103/148 (69.59%), Query Frame = 0


Query: 5   KIQLDLHFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP 64
           K  L   F +GFP +    V +A   W + T F+FT V ++  ADITI+F R NHGDGYP
Sbjct: 63  KRSLTYKFLTGFPTNGKPPVASACGRWAAVTPFTFTEVPSSDPADITITFARLNHGDGYP 122

Query: 65  FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA 124
           FDG GG LAHAFAPTDGRLH D DD Y +GA P  FD  SV VHE+GHI+GLGHSN  QA
Sbjct: 123 FDGKGGTLAHAFAPTDGRLHFDADDSYVIGAAPNAFDLESVAVHEMGHILGLGHSNVVQA 182

Query: 125 IMYPYINDGVVKATLQQDDLNGIRDLYG 153
           IMYP I+ G VK  LQ DD+NGI+ LYG
Sbjct: 183 IMYPSISSGTVKKELQADDINGIKALYG 210

[Close]

BLAST of MS024497 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 141.7 bits (356), Expect = 6.7e-33
Identity = 68/136 (50.00%), Postives = 83/136 (61.03%), Query Frame = 0


Query: 23  AVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYPFDGPGGVLAHAFAPTDGR 82
           A+  AF++W      +F    +   A+I I F   NHGD YPFDGPGG+L HAFAPTDGR
Sbjct: 167 AIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDGR 226

Query: 83  LHLDGDDRYAVGA------VPGNFDTSSVVVHEIGHIMGLGHSNDPQAIMYPYINDGVVK 142
            H D D+ +          V   FD  SV VHEIGH++GLGHS+D +AIMYP I     K
Sbjct: 227 CHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRK 286

Query: 143 ATLQQDDLNGIRDLYG 153
             L QDD++GIR LYG
Sbjct: 287 VNLAQDDIDGIRKLYG 302

[Close]

BLAST of MS024497 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.0e-28
Identity = 63/134 (47.01%), Postives = 79/134 (58.96%), Query Frame = 0


Query: 27  AFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYPFDGPGGVLAHAFAPTDGRLHLD 86
           AF  W+  T  +FTRV     +DI+I F  G HGDG PFDGP   LAHAF+P  G  HLD
Sbjct: 196 AFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLD 255

Query: 87  GDDRYAVGAVPGN--------FDTSSVVVHEIGHIMGLGHSNDPQAIMYPYINDGVVKAT 146
           G++ + V    G+         D  SV VHEIGH++GLGHS+   +IMYP I  G  K  
Sbjct: 256 GEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVD 315

Query: 147 LQQDDLNGIRDLYG 153
           L  DD+ G++ LYG
Sbjct: 316 LTTDDVEGVQYLYG 329

[Close]

BLAST of MS024497 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.7e-28
Identity = 63/133 (47.37%), Postives = 79/133 (59.40%), Query Frame = 0


Query: 27  AFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYPFDGPGGVLAHAFAPTDGRLHLD 86
           AF  W   T  +FT   +   +DITI F  G+HGDG PFDG  G LAHAF+P  G+ HLD
Sbjct: 192 AFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLD 251

Query: 87  GDDRYAVG-------AVPGNFDTSSVVVHEIGHIMGLGHSNDPQAIMYPYINDGVVKATL 146
            D+ + V        +V    D  SV VHEIGH++GLGHS+  ++IMYP I  G  K  L
Sbjct: 252 ADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDL 311

Query: 147 QQDDLNGIRDLYG 153
             DD+ GI+ LYG
Sbjct: 312 TNDDVEGIQYLYG 324

[Close]

BLAST of MS024497 vs. ExPASy Swiss-Prot
Match: P57748 (Matrix metalloproteinase-20 OS=Mus musculus OX=10090 GN=Mmp20 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.7e-28
Identity = 64/151 (42.38%), Postives = 89/151 (58.94%), Query Frame = 0


Query: 7   QLDLHFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYPFD 66
           ++  + PS  P +   A+  A + W +    +F R+ N+  ADI ISF+ G+HGD YPFD
Sbjct: 124 RISKYTPSMSPTEVDKAIQMALHAWSTAVPLNFVRI-NSGEADIMISFETGDHGDSYPFD 183

Query: 67  GPGGVLAHAFAPTD---GRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQ 126
           GP G LAHAFAP +   G  H D  +++ +G     F+  +V  HE GH +GLGHS DP 
Sbjct: 184 GPRGTLAHAFAPGEGLGGDTHFDNAEKWTMGT--NGFNLFTVAAHEFGHALGLGHSTDPS 243

Query: 127 AIMYP-YINDGVVKATLQQDDLNGIRDLYGP 154
           A+MYP Y      +  L +DD+ GI+ LYGP
Sbjct: 244 ALMYPTYKYQNPYRFHLPKDDVKGIQALYGP 271

[Close]

BLAST of MS024497 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.2e-28
Identity = 65/135 (48.15%), Postives = 80/135 (59.26%), Query Frame = 0


Query: 27  AFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYPFDGPGGVLAHAFAPTDGRLHLD 86
           AF  W   T  +FTR  + +RADI I F  G HGDG PFDG  G LAHA +P  G LHLD
Sbjct: 180 AFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLD 239

Query: 87  GDDRYAVG---------AVPGNFDTSSVVVHEIGHIMGLGHSNDPQAIMYPYINDGVVKA 146
           GD+ + +           V    D  SV VHEIGH++GLGHS+   AIM+P I+ G  K 
Sbjct: 240 GDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKV 299

Query: 147 TLQQDDLNGIRDLYG 153
            L +DD+ GI+ LYG
Sbjct: 300 ELAKDDIEGIQHLYG 314

[Close]

BLAST of MS024497 vs. ExPASy TrEMBL
Match: A0A6J1C1I2 (metalloendoproteinase 3-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006559 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 4.9e-79
Identity = 143/149 (95.97%), Postives = 144/149 (96.64%), Query Frame = 0


Query: 5   KIQLDLHFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP 64
           K  L  +FPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP
Sbjct: 65  KYSLTYNFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP 124

Query: 65  FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA 124
           FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA
Sbjct: 125 FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA 184

Query: 125 IMYPYINDGVVKATLQQDDLNGIRDLYGP 154
           IMYPYINDGVVKATLQQDDLNGIR LYGP
Sbjct: 185 IMYPYINDGVVKATLQQDDLNGIRALYGP 213

[Close]

BLAST of MS024497 vs. ExPASy TrEMBL
Match: A0A6J1H9C5 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460805 PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 6.1e-45
Identity = 89/148 (60.14%), Postives = 105/148 (70.95%), Query Frame = 0


Query: 5   KIQLDLHFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP 64
           K  L   F +GFP +    V +A   W + T+F+FT V ++  ADITI+F R +HGDGYP
Sbjct: 63  KRNLTYKFLTGFPTNGKPPVASACGRWAAVTQFTFTEVPSSAPADITITFARLSHGDGYP 122

Query: 65  FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA 124
           FDGPGG LAHAFAPTDGRLH D DD Y +GA P  FD  SV VHE+GHI+GLGHSN  QA
Sbjct: 123 FDGPGGTLAHAFAPTDGRLHFDADDSYVIGAAPNAFDLESVAVHEMGHILGLGHSNVVQA 182

Query: 125 IMYPYINDGVVKATLQQDDLNGIRDLYG 153
           IMYP I+ G VK  LQ DD+NGI+ LYG
Sbjct: 183 IMYPSISSGTVKKELQADDINGIKALYG 210

[Close]

BLAST of MS024497 vs. ExPASy TrEMBL
Match: A0A6J1JKI7 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111487737 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.4e-44
Identity = 89/148 (60.14%), Postives = 104/148 (70.27%), Query Frame = 0


Query: 5   KIQLDLHFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP 64
           K  L+  F +GFP +    V +A   W + T F+FT V ++  ADITI+F R NHGDGY 
Sbjct: 63  KRNLNYKFLTGFPTNGKPPVASACGRWAAVTPFTFTEVPSSAPADITITFARLNHGDGYS 122

Query: 65  FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA 124
           FDG GG LAHAFAPTDGRLH D DD Y +GA PG FD  SV VHE+GHI+GLGHS  PQA
Sbjct: 123 FDGKGGTLAHAFAPTDGRLHFDADDPYVIGAAPGAFDLESVAVHEMGHILGLGHSTVPQA 182

Query: 125 IMYPYINDGVVKATLQQDDLNGIRDLYG 153
           IMYP I+ G VK  LQ DD+NGI+ LYG
Sbjct: 183 IMYPSISSGTVKKDLQADDINGIKALYG 210

[Close]

BLAST of MS024497 vs. ExPASy TrEMBL
Match: A0A103D5M8 (Metallopeptidase, catalytic domain-containing protein (Fragment) OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_024147 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 9.1e-41
Identity = 88/151 (58.28%), Postives = 105/151 (69.54%), Query Frame = 0


Query: 5   KIQLDLHFPSGFPQDKVGAVVAAFNEWDSKTK-FSFTRVANNVRADITISFQRGNHGDGY 64
           K  L   F SGFP +++  +V AFN W S T+ F+F+RV N + ADI +SFQRGNHGDG 
Sbjct: 76  KSDLTYAFASGFPNNRIPPIVRAFNRWSSGTRYFTFSRVNNIMGADIKVSFQRGNHGDGA 135

Query: 65  PFDGPGGVLAHAFAPTDGRLHLDGDDRYA--VGAVPGNFDTSSVVVHEIGHIMGLGHSND 124
            FDGPGG+LAHAFAPTDGR+H D DD ++   G VP   D  +V VHEIGH++GL HSND
Sbjct: 136 SFDGPGGILAHAFAPTDGRVHYDADDTFSNGPGPVPNVIDFETVSVHEIGHLLGLDHSND 195

Query: 125 PQAIMYPYINDGVVKATLQQDDLNGIRDLYG 153
             A MY YI  GVVK  L  DD+ GIR LYG
Sbjct: 196 MNAAMYAYIYPGVVKG-LNADDIEGIRVLYG 225

[Close]

BLAST of MS024497 vs. ExPASy TrEMBL
Match: A0A6I9SUH3 (metalloendoproteinase 1 OS=Sesamum indicum OX=4182 GN=LOC105158120 PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 1.2e-40
Identity = 81/147 (55.10%), Postives = 104/147 (70.75%), Query Frame = 0


Query: 5   KIQLDLHFPSGFPQDKVGAVVAAFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYP 64
           K  L   FPSG P D   +++ A   W S + F+F+   +  +ADI ISFQ  +HGDG P
Sbjct: 164 KRSLTYSFPSGAPTDVTNSILHATQIWASVSPFTFSYTPDYDQADIKISFQNRDHGDGNP 223

Query: 65  FDGPGGVLAHAFAPTDGRLHLDGDDRYAVGAVPGNFDTSSVVVHEIGHIMGLGHSNDPQA 124
           FDGPGG+LAHA+AP+DGRLH DGD+R+  G  PG FD  +V +HE+GH++GLGHSND  A
Sbjct: 224 FDGPGGILAHAYAPSDGRLHFDGDERWVDGVTPGAFDLQTVGLHELGHVLGLGHSNDTGA 283

Query: 125 IMYPYINDGVVKATLQQDDLNGIRDLY 152
           +MYPYI DG+ K  L QDD+NGI+ LY
Sbjct: 284 VMYPYIGDGLRK-VLGQDDINGIKALY 309

[Close]

BLAST of MS024497 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 127.9 bits (320), Expect = 7.1e-30
Identity = 63/134 (47.01%), Postives = 79/134 (58.96%), Query Frame = 0


Query: 27  AFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYPFDGPGGVLAHAFAPTDGRLHLD 86
           AF  W+  T  +FTRV     +DI+I F  G HGDG PFDGP   LAHAF+P  G  HLD
Sbjct: 196 AFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLD 255

Query: 87  GDDRYAVGAVPGN--------FDTSSVVVHEIGHIMGLGHSNDPQAIMYPYINDGVVKAT 146
           G++ + V    G+         D  SV VHEIGH++GLGHS+   +IMYP I  G  K  
Sbjct: 256 GEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVD 315

Query: 147 LQQDDLNGIRDLYG 153
           L  DD+ G++ LYG
Sbjct: 316 LTTDDVEGVQYLYG 329

[Close]

BLAST of MS024497 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 127.1 bits (318), Expect = 1.2e-29
Identity = 63/133 (47.37%), Postives = 79/133 (59.40%), Query Frame = 0


Query: 27  AFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYPFDGPGGVLAHAFAPTDGRLHLD 86
           AF  W   T  +FT   +   +DITI F  G+HGDG PFDG  G LAHAF+P  G+ HLD
Sbjct: 192 AFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLD 251

Query: 87  GDDRYAVG-------AVPGNFDTSSVVVHEIGHIMGLGHSNDPQAIMYPYINDGVVKATL 146
            D+ + V        +V    D  SV VHEIGH++GLGHS+  ++IMYP I  G  K  L
Sbjct: 252 ADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDL 311

Query: 147 QQDDLNGIRDLYG 153
             DD+ GI+ LYG
Sbjct: 312 TNDDVEGIQYLYG 324

[Close]

BLAST of MS024497 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 126.7 bits (317), Expect = 1.6e-29
Identity = 65/135 (48.15%), Postives = 80/135 (59.26%), Query Frame = 0


Query: 27  AFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYPFDGPGGVLAHAFAPTDGRLHLD 86
           AF  W   T  +FTR  + +RADI I F  G HGDG PFDG  G LAHA +P  G LHLD
Sbjct: 180 AFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLD 239

Query: 87  GDDRYAVG---------AVPGNFDTSSVVVHEIGHIMGLGHSNDPQAIMYPYINDGVVKA 146
           GD+ + +           V    D  SV VHEIGH++GLGHS+   AIM+P I+ G  K 
Sbjct: 240 GDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKV 299

Query: 147 TLQQDDLNGIRDLYG 153
            L +DD+ GI+ LYG
Sbjct: 300 ELAKDDIEGIQHLYG 314

[Close]

BLAST of MS024497 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 125.6 bits (314), Expect = 3.5e-29
Identity = 62/132 (46.97%), Postives = 78/132 (59.09%), Query Frame = 0


Query: 27  AFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYPFDGPGGVLAHAFAPTDGRLHLD 86
           AF++W S    SF  V +   AD+ I F  G+HGDG PFDG  G LAHAFAP +GRLHLD
Sbjct: 189 AFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLD 248

Query: 87  GDDRYAV-----GAVPGNFDTSSVVVHEIGHIMGLGHSNDPQAIMYPYINDGVVKATLQQ 146
             + + V     G+     D  SV  HEIGH++GLGHS+   A+MYP +     K  L  
Sbjct: 249 AAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTV 308

Query: 147 DDLNGIRDLYGP 154
           DD+ G+  LYGP
Sbjct: 309 DDVAGVLKLYGP 320

[Close]

BLAST of MS024497 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 119.4 bits (298), Expect = 2.5e-27
Identity = 62/130 (47.69%), Postives = 76/130 (58.46%), Query Frame = 0


Query: 27  AFNEWDSKTKFSFTRVANNVRADITISFQRGNHGDGYPFDGPGGVLAHAFAPTDGRLHLD 86
           AF +W S    SF    + V ADI I F  G+HGDG PFDG  GVLAH F+P +GRLHLD
Sbjct: 167 AFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLD 226

Query: 87  GDDRYAVGAVPGN----FDTSSVVVHEIGHIMGLGHSNDPQAIMYPYINDGVVKATLQQD 146
             + +AV           D  SV VHEIGH++GLGHS+   A MYP +     K  L  D
Sbjct: 227 KAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMD 286

Query: 147 DLNGIRDLYG 153
           D+ G++ LYG
Sbjct: 287 DVVGVQSLYG 296

The following BLAST results are available for this feature:

BLAST of MS024497 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134258.1	1.0e-78	95.97	metalloendoproteinase 3-MMP-like [Momordica charantia]	[more]
XP_022959874.1	1.3e-44	60.14	metalloendoproteinase 1-like [Cucurbita moschata]	[more]
KAG6602460.1	1.3e-44	60.14	Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022991022.1	2.8e-44	60.14	metalloendoproteinase 2-MMP-like [Cucurbita maxima] >XP_022991023.1 metalloendop...	[more]
XP_023544550.1	1.1e-43	60.14	metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS024497 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P29136	6.7e-33	50.00	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
Q5XF51	1.0e-28	47.01	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
O04529	1.7e-28	47.37	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
P57748	1.7e-28	42.38	Matrix metalloproteinase-20 OS=Mus musculus OX=10090 GN=Mmp20 PE=2 SV=1	[more]
Q9ZUJ5	2.2e-28	48.15	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]

BLAST of MS024497 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C1I2	4.9e-79	95.97	metalloendoproteinase 3-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006559 ...	[more]
A0A6J1H9C5	6.1e-45	60.14	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460805 PE=4 ...	[more]
A0A6J1JKI7	1.4e-44	60.14	metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111487737 PE=...	[more]
A0A103D5M8	9.1e-41	58.28	Metallopeptidase, catalytic domain-containing protein (Fragment) OS=Cynara cardu...	[more]
A0A6I9SUH3	1.2e-40	55.10	metalloendoproteinase 1 OS=Sesamum indicum OX=4182 GN=LOC105158120 PE=3 SV=1	[more]

BLAST of MS024497 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24140.1	7.1e-30	47.01	Matrixin family protein	[more]
AT1G70170.1	1.2e-29	47.37	matrix metalloproteinase	[more]
AT1G59970.1	1.6e-29	48.15	Matrixin family protein	[more]
AT4G16640.1	3.5e-29	46.97	Matrixin family protein	[more]
AT2G45040.1	2.5e-27	47.69	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 139..152 score: 57.67 coord: 48..76 score: 66.86 coord: 105..130 score: 55.49 coord: 24..39 score: 30.03
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 2..153 e-value: 8.1E-29 score: 111.7
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 3..153 e-value: 7.3E-49 score: 168.4
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 22..152 e-value: 3.5E-44 score: 150.4
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 22..152
None	No IPR available	PANTHER	PTHR10201:SF268	METALLOENDOPROTEINASE 1-LIKE	coord: 22..152
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 12..152
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 10..152 e-value: 1.55568E-59 score: 179.707

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS024497.1	MS024497.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0008270	zinc ion binding