MS024341 (gene) Bitter gourd (TR) v1

Overview


Name	MS024341
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ribonuclease T(2)
Location	scaffold318: 199983 .. 200387 (-)
RNA-Seq Expression	MS024341
Synteny	MS024341

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCGAATACTGGCAAGACCTAGTTAGCGGGGACAACACCACGGCTTGGCAGACCAAATGGGCAATCCACGGGACTTGCTTGGCCGCGGTGTTCGATGAGTTCAAGTACTTTTGCTTGGGTTTGGATACGTACGGCCGCCATGCTGTCGTGTCATTTCTGGTGCACGGTGGCCTGGTCTCGAGCTCTGCTCAGCTGCATGACAAGACTGGCTTTATCAAAGCCATCGCCGATGTGACCTCGAAAAAGGGCGGCGTCATCTGCGGGGCCGACCAGCGGGGCCGAACACAGCTGCAGAAGGTCGTTTTGTGTTATAGTAAAGATGGTGCAACAATGATCGACTGCCTTGATAATGTCAGTAGTTCTTGCCCTGATAAGTTCTTCTGGCTTGCCTTGGGTGATTGA

mRNA sequence

ATGGGCGAATACTGGCAAGACCTAGTTAGCGGGGACAACACCACGGCTTGGCAGACCAAATGGGCAATCCACGGGACTTGCTTGGCCGCGGTGTTCGATGAGTTCAAGTACTTTTGCTTGGGTTTGGATACGTACGGCCGCCATGCTGTCGTGTCATTTCTGGTGCACGGTGGCCTGGTCTCGAGCTCTGCTCAGCTGCATGACAAGACTGGCTTTATCAAAGCCATCGCCGATGTGACCTCGAAAAAGGGCGGCGTCATCTGCGGGGCCGACCAGCGGGGCCGAACACAGCTGCAGAAGGTCGTTTTGTGTTATAGTAAAGATGGTGCAACAATGATCGACTGCCTTGATAATGTCAGTAGTTCTTGCCCTGATAAGTTCTTCTGGCTTGCCTTGGGTGATTGA

Coding sequence (CDS)

ATGGGCGAATACTGGCAAGACCTAGTTAGCGGGGACAACACCACGGCTTGGCAGACCAAATGGGCAATCCACGGGACTTGCTTGGCCGCGGTGTTCGATGAGTTCAAGTACTTTTGCTTGGGTTTGGATACGTACGGCCGCCATGCTGTCGTGTCATTTCTGGTGCACGGTGGCCTGGTCTCGAGCTCTGCTCAGCTGCATGACAAGACTGGCTTTATCAAAGCCATCGCCGATGTGACCTCGAAAAAGGGCGGCGTCATCTGCGGGGCCGACCAGCGGGGCCGAACACAGCTGCAGAAGGTCGTTTTGTGTTATAGTAAAGATGGTGCAACAATGATCGACTGCCTTGATAATGTCAGTAGTTCTTGCCCTGATAAGTTCTTCTGGCTTGCCTTGGGTGATTGA

Protein sequence

MGEYWQDLVSGDNTTAWQTKWAIHGTCLAAVFDEFKYFCLGLDTYGRHAVVSFLVHGGLVSSSAQLHDKTGFIKAIADVTSKKGGVICGADQRGRTQLQKVVLCYSKDGATMIDCLDNVSSSCPDKFFWLALGD

Homology

[Close]

BLAST of MS024341 vs. NCBI nr
Match: XP_022143591.1 (ribonuclease 1-like [Momordica charantia])

HSP 1 Score: 260.4 bits (664), Expect = 8.6e-66
Identity = 120/134 (89.55%), Postives = 124/134 (92.54%), Query Frame = 0


Query: 1   MGEYWQDLVSGDNTTAWQTKWAIHGTCLAAVFDEFKYFCLGLDTYGRHAVVSFLVHGGLV 60
           MGEYWQDLVSGDNT AWQTKWAIHGTC A VFDEFKYFC+GLDTYGRHA++SFLVHGGLV
Sbjct: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60

Query: 61  SSSAQLHDKTGFIKAIADVTSKKGGVICGADQRGRTQLQKVVLCYSKDGATMIDCLDNVS 120
            SSAQLH KT FIKAIADVTSKKGGVIC ADQRGRTQL+ VVLCYSKDGATMIDC DNVS
Sbjct: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120

Query: 121 SSCPDKFFWLALGD 135
           SSCPDKF WLALGD
Sbjct: 121 SSCPDKFLWLALGD 134

[Close]

BLAST of MS024341 vs. NCBI nr
Match: XP_038899255.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 228.4 bits (581), Expect = 3.6e-56
Identity = 103/134 (76.87%), Postives = 115/134 (85.82%), Query Frame = 0


Query: 1   MGEYWQDLVSGDNTTAWQTKWAIHGTCLAAVFDEFKYFCLGLDTYGRHAVVSFLVHGGLV 60
           M +YWQDL +G+   AW +KWAIHGTC A VFDEFKYFCLGLDTYGRHA++SFLVHGGLV
Sbjct: 88  MSQYWQDLENGNPIEAWASKWAIHGTCSATVFDEFKYFCLGLDTYGRHAMLSFLVHGGLV 147

Query: 61  SSSAQLHDKTGFIKAIADVTSKKGGVICGADQRGRTQLQKVVLCYSKDGATMIDCLDNVS 120
            SS++  DKT F+KAIAD+TSKKGGVIC  DQRGRTQLQK VLCY+KDG T+IDC DNVS
Sbjct: 148 PSSSKYLDKTSFVKAIADMTSKKGGVICAVDQRGRTQLQKAVLCYAKDGDTLIDCPDNVS 207

Query: 121 SSCPDKFFWLALGD 135
           SSCPD F WLALGD
Sbjct: 208 SSCPDNFLWLALGD 221

[Close]

BLAST of MS024341 vs. NCBI nr
Match: XP_004149779.2 (ribonuclease MC [Cucumis sativus])

HSP 1 Score: 190.3 bits (482), Expect = 1.1e-44
Identity = 88/134 (65.67%), Postives = 101/134 (75.37%), Query Frame = 0


Query: 1   MGEYWQDLVSGDNTTAWQTKWAIHGTCLAAVFDEFKYFCLGLDTYGRHAVVSFLVHGGLV 60
           M +YW+DL +G++  AW +KW +HGTC AA FD+FKYFCLGLDTYGRHA+ SFL   GL 
Sbjct: 88  MNQYWKDLENGNSIEAWASKWEMHGTCSAAGFDQFKYFCLGLDTYGRHAIFSFLDREGLA 147

Query: 61  SSSAQLHDKTGFIKAIADVTSKKGGVICGADQRGRTQLQKVVLCYSKDGATMIDCLDNVS 120
            SS++   K  FI AIA+ T KKGGVIC  DQ  R QLQK VLCY KDG T+IDC DNVS
Sbjct: 148 PSSSKYVAKASFITAIANSTLKKGGVICAVDQYRRIQLQKAVLCYVKDGHTLIDCPDNVS 207

Query: 121 SSCPDKFFWLALGD 135
           SSCPD F WLALGD
Sbjct: 208 SSCPDNFVWLALGD 221

[Close]

BLAST of MS024341 vs. NCBI nr
Match: KGN45018.2 (hypothetical protein Csa_016393 [Cucumis sativus])

HSP 1 Score: 190.3 bits (482), Expect = 1.1e-44
Identity = 88/134 (65.67%), Postives = 101/134 (75.37%), Query Frame = 0


Query: 1   MGEYWQDLVSGDNTTAWQTKWAIHGTCLAAVFDEFKYFCLGLDTYGRHAVVSFLVHGGLV 60
           M +YW+DL +G++  AW +KW +HGTC AA FD+FKYFCLGLDTYGRHA+ SFL   GL 
Sbjct: 87  MNQYWKDLENGNSIEAWASKWEMHGTCSAAGFDQFKYFCLGLDTYGRHAIFSFLDREGLA 146

Query: 61  SSSAQLHDKTGFIKAIADVTSKKGGVICGADQRGRTQLQKVVLCYSKDGATMIDCLDNVS 120
            SS++   K  FI AIA+ T KKGGVIC  DQ  R QLQK VLCY KDG T+IDC DNVS
Sbjct: 147 PSSSKYVAKASFITAIANSTLKKGGVICAVDQYRRIQLQKAVLCYVKDGHTLIDCPDNVS 206

Query: 121 SSCPDKFFWLALGD 135
           SSCPD F WLALGD
Sbjct: 207 SSCPDNFVWLALGD 220

[Close]

BLAST of MS024341 vs. NCBI nr
Match: XP_022934593.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 90.5 bits (223), Expect = 1.2e-14
Identity = 43/133 (32.33%), Postives = 69/133 (51.88%), Query Frame = 0


Query: 1   MGEYWQDLVSGDNTTAWQTKWAIHGTCLAAVFDEFKYFCLGLDTYGRHA--VVSFLVHGG 60
           +  YW D++ G N   WQ +W  HG C    F++ +YF + L+    H+  +++ L   G
Sbjct: 89  LNTYWPDVIQGKNQQFWQHEWDRHGICSNPPFNQLQYFQITLNIRTNHSYDLLTILNAAG 148

Query: 61  LVSSSAQLHDKTGFIKAIADVTSKKGGVIC--GADQRGRTQLQKVVLCYSKDGATMIDCL 120
           L  S   + +      AI   T KK G+ C   A  + + QL +++LC+ KDG T+IDC 
Sbjct: 149 LGPSDTNIREYKDIEGAIQAATGKKPGLRCNINAQSKEKKQLLEIILCFDKDGITLIDCT 208

Query: 121 DNVSSSCPDKFFW 130
           +    +CP +F W
Sbjct: 209 EFAGITCPPQFVW 221

[Close]

BLAST of MS024341 vs. ExPASy TrEMBL
Match: A0A6J1CQQ9 (ribonuclease 1-like OS=Momordica charantia OX=3673 GN=LOC111013449 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 4.2e-66
Identity = 120/134 (89.55%), Postives = 124/134 (92.54%), Query Frame = 0


Query: 1   MGEYWQDLVSGDNTTAWQTKWAIHGTCLAAVFDEFKYFCLGLDTYGRHAVVSFLVHGGLV 60
           MGEYWQDLVSGDNT AWQTKWAIHGTC A VFDEFKYFC+GLDTYGRHA++SFLVHGGLV
Sbjct: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60

Query: 61  SSSAQLHDKTGFIKAIADVTSKKGGVICGADQRGRTQLQKVVLCYSKDGATMIDCLDNVS 120
            SSAQLH KT FIKAIADVTSKKGGVIC ADQRGRTQL+ VVLCYSKDGATMIDC DNVS
Sbjct: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120

Query: 121 SSCPDKFFWLALGD 135
           SSCPDKF WLALGD
Sbjct: 121 SSCPDKFLWLALGD 134

[Close]

BLAST of MS024341 vs. ExPASy TrEMBL
Match: A0A0A0K4L1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G351900 PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.3e-45
Identity = 88/134 (65.67%), Postives = 101/134 (75.37%), Query Frame = 0


Query: 1   MGEYWQDLVSGDNTTAWQTKWAIHGTCLAAVFDEFKYFCLGLDTYGRHAVVSFLVHGGLV 60
           M +YW+DL +G++  AW +KW +HGTC AA FD+FKYFCLGLDTYGRHA+ SFL   GL 
Sbjct: 1   MNQYWKDLENGNSIEAWASKWEMHGTCSAAGFDQFKYFCLGLDTYGRHAIFSFLDREGLA 60

Query: 61  SSSAQLHDKTGFIKAIADVTSKKGGVICGADQRGRTQLQKVVLCYSKDGATMIDCLDNVS 120
            SS++   K  FI AIA+ T KKGGVIC  DQ  R QLQK VLCY KDG T+IDC DNVS
Sbjct: 61  PSSSKYVAKASFITAIANSTLKKGGVICAVDQYRRIQLQKAVLCYVKDGHTLIDCPDNVS 120

Query: 121 SSCPDKFFWLALGD 135
           SSCPD F WLALGD
Sbjct: 121 SSCPDNFVWLALGD 134

[Close]

BLAST of MS024341 vs. ExPASy TrEMBL
Match: A0A6J1F837 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 5.7e-15
Identity = 43/133 (32.33%), Postives = 69/133 (51.88%), Query Frame = 0


Query: 1   MGEYWQDLVSGDNTTAWQTKWAIHGTCLAAVFDEFKYFCLGLDTYGRHA--VVSFLVHGG 60
           +  YW D++ G N   WQ +W  HG C    F++ +YF + L+    H+  +++ L   G
Sbjct: 89  LNTYWPDVIQGKNQQFWQHEWDRHGICSNPPFNQLQYFQITLNIRTNHSYDLLTILNAAG 148

Query: 61  LVSSSAQLHDKTGFIKAIADVTSKKGGVIC--GADQRGRTQLQKVVLCYSKDGATMIDCL 120
           L  S   + +      AI   T KK G+ C   A  + + QL +++LC+ KDG T+IDC 
Sbjct: 149 LGPSDTNIREYKDIEGAIQAATGKKPGLRCNINAQSKEKKQLLEIILCFDKDGITLIDCT 208

Query: 121 DNVSSSCPDKFFW 130
           +    +CP +F W
Sbjct: 209 EFAGITCPPQFVW 221

[Close]

BLAST of MS024341 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.3e-14
Identity = 43/133 (32.33%), Postives = 70/133 (52.63%), Query Frame = 0


Query: 1   MGEYWQDLVSGDNTTAWQTKWAIHGTCLAAVFDEFKYF--CLGLDTYGRHAVVSFLVHGG 60
           +  YW D++SG+N   W+ +W  HGTC    F++ +YF   L + T   + +++ L   G
Sbjct: 85  LNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQLQYFQTTLNIRTNHNYDLLAILNTAG 144

Query: 61  LVSSSAQLHDKTGFIKAIADVTSKKGGVICGAD-QRGRTQLQKVVLCYSKDGATMIDCLD 120
           L  ++           AI   T KK G+ C  + Q  + QL +++LC+ K+G T+IDC  
Sbjct: 145 LGPTATNTRQYGAIEGAIQAATGKKPGLRCNKNAQSKKKQLFEIILCFDKNGVTLIDCTQ 204

Query: 121 NVSSSCPDKFFWL 131
               +CP +F WL
Sbjct: 205 FAGITCPSQFVWL 217

[Close]

BLAST of MS024341 vs. ExPASy TrEMBL
Match: A0A6J1IAR7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 6.3e-14
Identity = 42/133 (31.58%), Postives = 70/133 (52.63%), Query Frame = 0


Query: 1   MGEYWQDLVSGDNTTAWQTKWAIHGTCLAAVFDEFKYF--CLGLDTYGRHAVVSFLVHGG 60
           +  YW D+++G+N   W+ +W  HGTC    F++ +YF   L + T   + +++ L   G
Sbjct: 86  LNTYWPDVINGNNQRFWKHEWDNHGTCSDPPFNQLQYFQTTLNIRTNHNYDLLAILNTAG 145

Query: 61  LVSSSAQLHDKTGFIKAIADVTSKKGGVICGAD-QRGRTQLQKVVLCYSKDGATMIDCLD 120
           L  ++           AI   T KK G+ C  + Q  R QL +++LC+ K+G T+I+C  
Sbjct: 146 LGPTATTTRQYQAIEGAIQAATGKKPGLRCNKNAQSKRKQLFEIILCFDKNGVTLINCTQ 205

Query: 121 NVSSSCPDKFFWL 131
               +CP +F WL
Sbjct: 206 FAGITCPSQFVWL 218

The following BLAST results are available for this feature:

BLAST of MS024341 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143591.1	8.6e-66	89.55	ribonuclease 1-like [Momordica charantia]	[more]
XP_038899255.1	3.6e-56	76.87	ribonuclease MC-like [Benincasa hispida]	[more]
XP_004149779.2	1.1e-44	65.67	ribonuclease MC [Cucumis sativus]	[more]
KGN45018.2	1.1e-44	65.67	hypothetical protein Csa_016393 [Cucumis sativus]	[more]
XP_022934593.1	1.2e-14	32.33	ribonuclease MC-like [Cucurbita moschata]	[more]

BLAST of MS024341 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS024341 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQQ9	4.2e-66	89.55	ribonuclease 1-like OS=Momordica charantia OX=3673 GN=LOC111013449 PE=3 SV=1	[more]
A0A0A0K4L1	5.3e-45	65.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G351900 PE=3 SV=1	[more]
A0A6J1F837	5.7e-15	32.33	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1	[more]
A0A6J1EXD5	1.3e-14	32.33	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1	[more]
A0A6J1IAR7	6.3e-14	31.58	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1	[more]

BLAST of MS024341 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 1..130 e-value: 2.6E-22 score: 81.9
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 1..129
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 1..115 e-value: 3.7E-15 score: 56.5
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 3..121
None	No IPR available	PANTHER	PTHR11240:SF51	RIBONUCLEASE 2	coord: 3..121
None	No IPR available	CDD	cd00374	RNase_T2	coord: 1..130 e-value: 1.1449E-21 score: 83.6596

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS024341.1	MS024341.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding