MS024268 (gene) Bitter gourd (TR) v1

Overview
NameMS024268
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncytochrome P450 71A24-like
Locationscaffold30: 634649 .. 635767 (-)
RNA-Seq ExpressionMS024268
SyntenyMS024268
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAAACTCTTCAATATCAGACGCAGCCTCGCCCCCATCTCCGCCAAAAATCCCCATTTTCGGGCACCTTCTGAACCTGGGTTCCCTCCCCCATATCACTCTTCAAGCCTATGCCCGGCACCATGGCCCTCTGTTGCTGATTCACCTCGGCAGCAAGCCCACCATCGTGGTCTCTTCCGCCGAGATCGCTTAGGAAATCATGAAGACCCACGACCTGATCTTCGCCGACCGCCCCAAATCAAGCTTAAGCGACAAGCTTCTCTACGACTCCAGAGATATAGCCGCATCCCCTTACGGCGAATACTGAAGGCAGATGAAGAGCATTGCAGTTCTTCATCTCTTGAGCAACAAAAGGGTTCACTCCTTTCGCTCCGTCAGAGAAGAGGAAGTCAAATTGATGATCCAAAACATCCGACAGAATCCGGCGTCGGTGAATTTAAGCCACGAATTCTCTAGACTGACGAATGATGTGATTTGCAGGGTGGCTTTAGGGAGGCTGTGGCGGGGGAAGATGGCCGGAATAAGTTCCGGGATCTTCTTCCTCAATTTATGAAATTATTGGGGAGTTTCATTGTAAGAGACTTGATTCCGTGGCTGGGTTGGATTGATGGGATCAGTGGGATGGATGCCAAGGCAAAGAGGGTAGCCAAAGAACTTGATGATTTGATTGACAGAGTGATTCAAGATCATGTCCATCCAAATTACAACCCCGAGGAGCAGAGCAGGGACTTGGTGGACGTGCTGCTTTCAATACAGAGACAGGACTCCATCGGATTTCCCCTTGAGATGAAAAGCATAAAAGCTTTAATCCTGGTAATTTAGGTACTACAAGAAACAACCAACTAGTACACTATTACATGAAAGTTGAGACCTTTTTGTTTGGATTTTGCGTTACAGGACATGTTTGGAGGAGGCACGGACACTACATACACGGTATTGGAGTGGGCGATGTCAGAGCTTTTGAGACACCCAGAAGCTATGGAGAAATTACAGGGTGAGGTGAAAGAAGTAGCAGGGGAGAAAGAATACGTGATTGAGGATGATTTAGAGAAGATGCATTACTTGAAGGCAGTGATCAAGGAAACATTGCGATTACACCACCAATCCCACTACTAG

mRNA sequence

ATGGCTAAACTCTTCAATATCAGACGCAGCCTCGCCCCCATCTCCGCCAAAAATCCCCATTTTCGGGCACCTTCTGAACCTGGGTTCCCTCCCCCATATCACTCTTCAAGCCTATGCCCGGCACCATGGCCCTCTGTTGCTGATTCACCTCGGCAGCAAGCCCACCATCGTGGTCTCTTCCGCCGAGATCGCTTAGGAAATCATGAAGACCCACGACCTGATCTTCGCCGACCGCCCCAAATCAAGCTTAAGCGACAAGCTTCTCTACGACTCCAGAGATATAGCCGCATCCCCTTACGGCGAATACTGAAGGCAGATGAAGAGCATTGCAGTTCTTCATCTCTTGAGCAACAAAAGGGTTCACTCCTTTCGCTCCGTCAGAGAAGAGGAAGTCAAATTGATGATCCAAAACATCCGACAGAATCCGGCGGTGGCTTTAGGGAGGCTGTGGCGGGGGAAGATGGCCGGAATAAGTTCCGGGATCTTCTTCCTCAATTTATGAAATTATTGGGGAGTTTCATTGTAAGAGACTTGATTCCGTGGCTGGGTTGGATTGATGGGATCAGTGGGATGGATGCCAAGGCAAAGAGGGTAGCCAAAGAACTTGATGATTTGATTGACAGAGTGATTCAAGATCATGTCCATCCAAATTACAACCCCGAGGAGCAGAGCAGGGACTTGGTGGACGTGCTGCTTTCAATACAGAGACAGGACTCCATCGGATTTCCCCTTGAGATGAAAAGCATAAAAGCTTTAATCCTGGACATGTTTGGAGGAGGCACGGACACTACATACACGGTATTGGAGTGGGCGATGTCAGAGCTTTTGAGACACCCAGAAGCTATGGAGAAATTACAGGGTGAGGTGAAAGAAGTAGCAGGGGAGAAAGAATACGTGATTGAGGATGATTTAGAGAAGATGCATTACTTGAAGGCAGTGATCAAGGAAACATTGCGATTACACCACCAATCCCACTACTAG

Coding sequence (CDS)

ATGGCTAAACTCTTCAATATCAGACGCAGCCTCGCCCCCATCTCCGCCAAAAATCCCCATTTTCGGGCACCTTCTGAACCTGGGTTCCCTCCCCCATATCACTCTTCAAGCCTATGCCCGGCACCATGGCCCTCTGTTGCTGATTCACCTCGGCAGCAAGCCCACCATCGTGGTCTCTTCCGCCGAGATCGCTTAGGAAATCATGAAGACCCACGACCTGATCTTCGCCGACCGCCCCAAATCAAGCTTAAGCGACAAGCTTCTCTACGACTCCAGAGATATAGCCGCATCCCCTTACGGCGAATACTGAAGGCAGATGAAGAGCATTGCAGTTCTTCATCTCTTGAGCAACAAAAGGGTTCACTCCTTTCGCTCCGTCAGAGAAGAGGAAGTCAAATTGATGATCCAAAACATCCGACAGAATCCGGCGGTGGCTTTAGGGAGGCTGTGGCGGGGGAAGATGGCCGGAATAAGTTCCGGGATCTTCTTCCTCAATTTATGAAATTATTGGGGAGTTTCATTGTAAGAGACTTGATTCCGTGGCTGGGTTGGATTGATGGGATCAGTGGGATGGATGCCAAGGCAAAGAGGGTAGCCAAAGAACTTGATGATTTGATTGACAGAGTGATTCAAGATCATGTCCATCCAAATTACAACCCCGAGGAGCAGAGCAGGGACTTGGTGGACGTGCTGCTTTCAATACAGAGACAGGACTCCATCGGATTTCCCCTTGAGATGAAAAGCATAAAAGCTTTAATCCTGGACATGTTTGGAGGAGGCACGGACACTACATACACGGTATTGGAGTGGGCGATGTCAGAGCTTTTGAGACACCCAGAAGCTATGGAGAAATTACAGGGTGAGGTGAAAGAAGTAGCAGGGGAGAAAGAATACGTGATTGAGGATGATTTAGAGAAGATGCATTACTTGAAGGCAGTGATCAAGGAAACATTGCGATTACACCACCAATCCCACTACTAG

Protein sequence

MAKLFNIRRSLAPISAKNPHFRAPSEPGFPPPYHSSSLCPAPWPSVADSPRQQAHHRGLFRRDRLGNHEDPRPDLRRPPQIKLKRQASLRLQRYSRIPLRRILKADEEHCSSSSLEQQKGSLLSLRQRRGSQIDDPKHPTESGGGFREAVAGEDGRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWAMSELLRHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLHHQSHY
Homology
BLAST of MS024268 vs. NCBI nr
Match: XP_022134640.1 (cytochrome P450 71A24-like [Momordica charantia])

HSP 1 Score: 303.1 bits (775), Expect = 2.8e-78
Identity = 153/174 (87.93%), Postives = 161/174 (92.53%), Query Frame = 0

Query: 149 AVAGEDGRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDR 208
           AVAGED   KFRD+LPQF+KLLGSF VRD +PWLGWIDGISGMDAKAK+VAKELDD +DR
Sbjct: 199 AVAGED---KFRDILPQFVKLLGSFSVRDFVPWLGWIDGISGMDAKAKKVAKELDDFVDR 258

Query: 209 VIQDHVHPNYNPEEQSRDLVDVLLSIQRQDSIGF-PLEMKSIKALILDMFGGGTDTTYTV 268
           VIQDHVH +YNPEEQS+DLVDVLLSIQRQDSIGF PLEMKSIKALILDMF GGTDT YTV
Sbjct: 259 VIQDHVHADYNPEEQSQDLVDVLLSIQRQDSIGFPPLEMKSIKALILDMFAGGTDTIYTV 318

Query: 269 LEWAMSELLRHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
           LEW MSELLRHPEAM+KLQ EVKEVAGEKEYV EDDLEKMHYLKAVIKETLRLH
Sbjct: 319 LEWTMSELLRHPEAMKKLQSEVKEVAGEKEYVSEDDLEKMHYLKAVIKETLRLH 369

BLAST of MS024268 vs. NCBI nr
Match: XP_022134746.1 (cytochrome P450 71A26-like [Momordica charantia])

HSP 1 Score: 242.7 bits (618), Expect = 4.5e-60
Identity = 126/177 (71.19%), Postives = 145/177 (81.92%), Query Frame = 0

Query: 147 REAVAGEDGRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLI 206
           R+   GEDG+ KFR LL +F +LLGSF  RD IPWLGWIDGISG+DAKA+RVA E+D  +
Sbjct: 193 RKYGVGEDGK-KFRCLLLEFGELLGSFSGRDFIPWLGWIDGISGLDAKAERVANEIDAFL 252

Query: 207 DRVIQDHVHPNYNPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYT 266
           +RVI+DHV       ++ +DLVD+LLSIQ+QDSIGFPLEM SIKALILDMF  GTDTTYT
Sbjct: 253 NRVIEDHVETGEISVDKHKDLVDILLSIQKQDSIGFPLEMDSIKALILDMFAAGTDTTYT 312

Query: 267 VLEWAMSELLRHPEAMEKLQGEVKEVAG--EKEYVIEDDLEKMHYLKAVIKETLRLH 322
           VLEWAMS+LLRHPEAM+KLQ EV+E  G  EK  V EDDLE+MHYLKAVIKETLRLH
Sbjct: 313 VLEWAMSQLLRHPEAMKKLQTEVRETVGDREKSCVSEDDLERMHYLKAVIKETLRLH 368

BLAST of MS024268 vs. NCBI nr
Match: XP_022933473.1 (cytochrome P450 71A3-like [Cucurbita moschata])

HSP 1 Score: 241.5 bits (615), Expect = 1.0e-59
Identity = 121/170 (71.18%), Postives = 137/170 (80.59%), Query Frame = 0

Query: 152 GEDGRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQ 211
           GEDG  KFR LL QF + + SF +RD IPWLGWIDGISG+D +AKRVAKELD  +DRVIQ
Sbjct: 202 GEDG-TKFRSLLLQFGESMASFSIRDFIPWLGWIDGISGLDVQAKRVAKELDVFLDRVIQ 261

Query: 212 DHVHPNYNPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWA 271
           DHVHP    E    DLVD LL I+  DSIGFPLEM SIKALILDMF  GTDTTYT LEWA
Sbjct: 262 DHVHPENRDEH--NDLVDTLLCIKTGDSIGFPLEMDSIKALILDMFAAGTDTTYTALEWA 321

Query: 272 MSELLRHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
           MSELL+HPE+M+KL+ E+K + GEK YV E+D+EKMHYLKA+IKETLRLH
Sbjct: 322 MSELLKHPESMKKLKSEIKAIVGEKGYVEEEDIEKMHYLKAIIKETLRLH 368

BLAST of MS024268 vs. NCBI nr
Match: XP_022987235.1 (cytochrome P450 71A25-like [Cucurbita maxima])

HSP 1 Score: 235.3 bits (599), Expect = 7.2e-58
Identity = 120/166 (72.29%), Postives = 138/166 (83.13%), Query Frame = 0

Query: 158 KFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPN 217
           K R LL +F++LLGSF VRD IPWLGWIDGISG+DAKAKRVAKELD  +DRVIQDHV   
Sbjct: 206 KLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVIQDHVDSE 265

Query: 218 YNPEEQSR--DLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWAMSEL 277
              E   +  DLVDVLL IQR +SIGFPLEM SIKALILDMF  GTDTT TVLEWAMSEL
Sbjct: 266 NRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVLEWAMSEL 325

Query: 278 LRHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
            +HPEAM+KL+ E++E++G+ +YV EDDL+KMHYLKAV+KETLRLH
Sbjct: 326 FKHPEAMKKLKSEIREISGD-QYVNEDDLDKMHYLKAVMKETLRLH 370

BLAST of MS024268 vs. NCBI nr
Match: XP_023515571.1 (cytochrome P450 71A24-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 235.3 bits (599), Expect = 7.2e-58
Identity = 122/179 (68.16%), Postives = 143/179 (79.89%), Query Frame = 0

Query: 147 REAVAGEDGRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLI 206
           R+  AGEDG  K R LL + + LLGSF VRD IPWLGWIDGISG+DAK KRVAKELD  +
Sbjct: 196 RKFDAGEDGM-KLRSLLLELLDLLGSFGVRDFIPWLGWIDGISGLDAKGKRVAKELDVFL 255

Query: 207 DRVIQDHVHPNYNPEE--QSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTT 266
           DRVIQDHVH     E   + +DLVDVLL +QR +SIGFPLEM SIKALILDMF  GT+TT
Sbjct: 256 DRVIQDHVHSENRDENSGKHKDLVDVLLWVQRNNSIGFPLEMDSIKALILDMFAAGTETT 315

Query: 267 YTVLEWAMSELLRHPEAMEKLQGEVKEVAGEKE--YVIEDDLEKMHYLKAVIKETLRLH 322
            TVLEW MSELL+HPE M+KL+ E++E++G+ +  YV EDDL+KMHYLKAV+KETLRLH
Sbjct: 316 STVLEWTMSELLKHPEVMKKLKSEIREISGDHKAYYVNEDDLDKMHYLKAVLKETLRLH 373

BLAST of MS024268 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 7.8e-47
Identity = 100/235 (42.55%), Postives = 149/235 (63.40%), Query Frame = 0

Query: 89  LRLQRYSRIPLRRILKADEEHCSSSSLEQQKGSLLSLRQRRGSQIDDPKHPTESGGGFRE 148
           L L    R+   R ++ +E      +++    S+L+L +      +D       G  + +
Sbjct: 150 LNLLSNRRVRSFRSVREEETKSMIRNIKGSSSSVLNLSEMFVRLTNDVVCKVALGRKYSD 209

Query: 149 AVAGEDGRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDR 208
              GE GR  F+++L +F  LLG+  + D +PWL W+  ++G+ AK  +VAK+LDD ID 
Sbjct: 210 GEGGESGR-MFKEILGEFGDLLGTVNIGDYVPWLSWLSHVNGLGAKLDKVAKQLDDFIDT 269

Query: 209 VIQDHV-HPNYNPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTV 268
           V+Q+H+ H + + ++  +D +D+LL+IQ++ S G P++  S+K +ILDMF  GTDTTY+ 
Sbjct: 270 VVQEHMNHSSRSGDDDQKDFLDILLAIQKETSAGIPIDGVSVKGIILDMFAAGTDTTYSA 329

Query: 269 LEWAMSELLRHPEAMEKLQGEVKEVAGEKEYVI-EDDLEKMHYLKAVIKETLRLH 322
           LEWAM+ELLRHP  M KLQ EV+ + G +  VI EDDL +MHYLKAV KETLRLH
Sbjct: 330 LEWAMTELLRHPRVMNKLQNEVRGIVGNRTDVITEDDLVEMHYLKAVTKETLRLH 383

BLAST of MS024268 vs. ExPASy Swiss-Prot
Match: P37119 (Cytochrome P450 71A3 (Fragment) OS=Solanum melongena OX=4111 GN=CYP71A3 PE=2 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 2.1e-44
Identity = 93/162 (57.41%), Postives = 117/162 (72.22%), Query Frame = 0

Query: 163 LPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNPEE 222
           L +F++LLG+F V D IP L W++ I+G D+K  +VAK+LD  ++ VI+ H+  N   E 
Sbjct: 196 LDKFVELLGTFNVGDYIPCLEWVNKITGFDSKVDKVAKDLDTFLEFVIEAHMIRNEKEEN 255

Query: 223 ---QSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWAMSELLRHP 282
              +S+DLVDVLL IQ     GFP++  S+KAL+LD F  GTDT YTVLEW M+ELLRHP
Sbjct: 256 RAGESKDLVDVLLEIQNGKETGFPIQRDSLKALLLDPFSAGTDTIYTVLEWTMTELLRHP 315

Query: 283 EAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
            AMEKLQ EV+ +A EK  + EDDL  MHYLKAVIKETLRLH
Sbjct: 316 RAMEKLQNEVQGLAQEKAEITEDDLPNMHYLKAVIKETLRLH 357

BLAST of MS024268 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 176.8 bits (447), Expect = 4.0e-43
Identity = 87/172 (50.58%), Postives = 117/172 (68.02%), Query Frame = 0

Query: 153 EDGRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQD 212
           E+ +NKF DL  +   L+G+F V D  P   W+D ++GMDA+ KR   ELD  +D VI D
Sbjct: 195 EERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAFVDHVIDD 254

Query: 213 HV---HPNYNPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLE 272
           H+     N +   + +DLVDVLL +Q+  S+G  L   ++KA+ILDMF GGTDTT   LE
Sbjct: 255 HLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSGGTDTTAVTLE 314

Query: 273 WAMSELLRHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
           WAM+EL++HP+ MEK Q EV+ V G+K  V E+DL ++HYLK +IKETLRLH
Sbjct: 315 WAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLRLH 366

BLAST of MS024268 vs. ExPASy Swiss-Prot
Match: Q42716 (Cytochrome P450 71A8 OS=Mentha piperita OX=34256 GN=CYP71A8 PE=3 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.5e-42
Identity = 114/282 (40.43%), Postives = 169/282 (59.93%), Query Frame = 0

Query: 61  RRDRLGNHEDPRPDLRRPPQIKLKRQASLRLQ-------RYSRIPLRRI----LKADEEH 120
           RR   GNHE+ R  LRRP   +  R A+L+LQ        Y R  L+ I    L +++  
Sbjct: 97  RRRPRGNHENSRSRLRRP---RGSRSAALQLQGRVGGYGEYWR-QLKTICVVQLLSNKRV 156

Query: 121 CSSSSLEQQKGSLLSLRQRRGSQIDDPKH---------PTESGGGFREAVAGEDGRNKFR 180
            S  S+ +++  LL  +    S   +  H            S  G R+  AG++   KF 
Sbjct: 157 QSFRSVREEETELLMKKIGDSSGNVNLSHMFTQLTNDVVCRSAIG-RKYGAGDENGEKFL 216

Query: 181 DLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNYNP 240
           ++L +F++LLG+  + D +P L WI+ I+G D +  R+AKE+D+ +++VI + +    NP
Sbjct: 217 EILREFLELLGAISIGDFVPSLWWINRINGFDRRVDRIAKEMDEFLEKVIHERLE---NP 276

Query: 241 EEQSRD-LVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWAMSELLRHP 300
             ++ +  VD+LL I R +S G  ++  SIKA+ILD+F  GTDTT  VLEWAM+ELLRHP
Sbjct: 277 AAKAEENFVDILLEIYRNNSAGVSIDRDSIKAIILDVFAAGTDTTAVVLEWAMTELLRHP 336

Query: 301 EAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
           E M+KLQ EV++V  +K  + +DD+EKMHYLKAV+KET+R H
Sbjct: 337 EIMKKLQSEVRQVVKDKHNITDDDIEKMHYLKAVMKETMRFH 370

BLAST of MS024268 vs. ExPASy Swiss-Prot
Match: Q9STK9 (Cytochrome P450 71A24 OS=Arabidopsis thaliana OX=3702 GN=CYP71A24 PE=2 SV=3)

HSP 1 Score: 172.6 bits (436), Expect = 7.6e-42
Identity = 84/167 (50.30%), Postives = 118/167 (70.66%), Query Frame = 0

Query: 155 GRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHV 214
           G+  F+DL+ +  +LLG F V   + WL WID I G+D +  +++ +LD+ ++RV+QDHV
Sbjct: 193 GKTDFKDLMKRLTRLLGEFSVGSYVSWLAWIDWIRGLDGQLIKISNDLDEFLERVVQDHV 252

Query: 215 HPNYNPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWAMSE 274
               + +    D VD LL+I+R+ S+GF ++  SIKA+ILD+F G  DTTYT+LEWAM+E
Sbjct: 253 ----DGDGHKNDFVDFLLTIEREKSVGFEIDRLSIKAIILDVFVGDMDTTYTLLEWAMTE 312

Query: 275 LLRHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
           LL H E +++LQ EV+ V  +K  V EDDL+ M YLKAVIKETLRLH
Sbjct: 313 LLCHHECLDRLQEEVRMVCKDKSGVSEDDLQDMKYLKAVIKETLRLH 355

BLAST of MS024268 vs. ExPASy TrEMBL
Match: A0A6J1BYV5 (cytochrome P450 71A24-like OS=Momordica charantia OX=3673 GN=LOC111006861 PE=3 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 1.4e-78
Identity = 153/174 (87.93%), Postives = 161/174 (92.53%), Query Frame = 0

Query: 149 AVAGEDGRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDR 208
           AVAGED   KFRD+LPQF+KLLGSF VRD +PWLGWIDGISGMDAKAK+VAKELDD +DR
Sbjct: 199 AVAGED---KFRDILPQFVKLLGSFSVRDFVPWLGWIDGISGMDAKAKKVAKELDDFVDR 258

Query: 209 VIQDHVHPNYNPEEQSRDLVDVLLSIQRQDSIGF-PLEMKSIKALILDMFGGGTDTTYTV 268
           VIQDHVH +YNPEEQS+DLVDVLLSIQRQDSIGF PLEMKSIKALILDMF GGTDT YTV
Sbjct: 259 VIQDHVHADYNPEEQSQDLVDVLLSIQRQDSIGFPPLEMKSIKALILDMFAGGTDTIYTV 318

Query: 269 LEWAMSELLRHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
           LEW MSELLRHPEAM+KLQ EVKEVAGEKEYV EDDLEKMHYLKAVIKETLRLH
Sbjct: 319 LEWTMSELLRHPEAMKKLQSEVKEVAGEKEYVSEDDLEKMHYLKAVIKETLRLH 369

BLAST of MS024268 vs. ExPASy TrEMBL
Match: A0A6J1BYN7 (cytochrome P450 71A26-like OS=Momordica charantia OX=3673 GN=LOC111006942 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 2.2e-60
Identity = 126/177 (71.19%), Postives = 145/177 (81.92%), Query Frame = 0

Query: 147 REAVAGEDGRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLI 206
           R+   GEDG+ KFR LL +F +LLGSF  RD IPWLGWIDGISG+DAKA+RVA E+D  +
Sbjct: 193 RKYGVGEDGK-KFRCLLLEFGELLGSFSGRDFIPWLGWIDGISGLDAKAERVANEIDAFL 252

Query: 207 DRVIQDHVHPNYNPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYT 266
           +RVI+DHV       ++ +DLVD+LLSIQ+QDSIGFPLEM SIKALILDMF  GTDTTYT
Sbjct: 253 NRVIEDHVETGEISVDKHKDLVDILLSIQKQDSIGFPLEMDSIKALILDMFAAGTDTTYT 312

Query: 267 VLEWAMSELLRHPEAMEKLQGEVKEVAG--EKEYVIEDDLEKMHYLKAVIKETLRLH 322
           VLEWAMS+LLRHPEAM+KLQ EV+E  G  EK  V EDDLE+MHYLKAVIKETLRLH
Sbjct: 313 VLEWAMSQLLRHPEAMKKLQTEVRETVGDREKSCVSEDDLERMHYLKAVIKETLRLH 368

BLAST of MS024268 vs. ExPASy TrEMBL
Match: A0A6J1F4U4 (cytochrome P450 71A3-like OS=Cucurbita moschata OX=3662 GN=LOC111440879 PE=3 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 4.9e-60
Identity = 121/170 (71.18%), Postives = 137/170 (80.59%), Query Frame = 0

Query: 152 GEDGRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQ 211
           GEDG  KFR LL QF + + SF +RD IPWLGWIDGISG+D +AKRVAKELD  +DRVIQ
Sbjct: 202 GEDG-TKFRSLLLQFGESMASFSIRDFIPWLGWIDGISGLDVQAKRVAKELDVFLDRVIQ 261

Query: 212 DHVHPNYNPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWA 271
           DHVHP    E    DLVD LL I+  DSIGFPLEM SIKALILDMF  GTDTTYT LEWA
Sbjct: 262 DHVHPENRDEH--NDLVDTLLCIKTGDSIGFPLEMDSIKALILDMFAAGTDTTYTALEWA 321

Query: 272 MSELLRHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
           MSELL+HPE+M+KL+ E+K + GEK YV E+D+EKMHYLKA+IKETLRLH
Sbjct: 322 MSELLKHPESMKKLKSEIKAIVGEKGYVEEEDIEKMHYLKAIIKETLRLH 368

BLAST of MS024268 vs. ExPASy TrEMBL
Match: A0A6J1JG96 (cytochrome P450 71A25-like OS=Cucurbita maxima OX=3661 GN=LOC111484850 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 3.5e-58
Identity = 120/166 (72.29%), Postives = 138/166 (83.13%), Query Frame = 0

Query: 158 KFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPN 217
           K R LL +F++LLGSF VRD IPWLGWIDGISG+DAKAKRVAKELD  +DRVIQDHV   
Sbjct: 206 KLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVIQDHVDSE 265

Query: 218 YNPEEQSR--DLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWAMSEL 277
              E   +  DLVDVLL IQR +SIGFPLEM SIKALILDMF  GTDTT TVLEWAMSEL
Sbjct: 266 NRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVLEWAMSEL 325

Query: 278 LRHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
            +HPEAM+KL+ E++E++G+ +YV EDDL+KMHYLKAV+KETLRLH
Sbjct: 326 FKHPEAMKKLKSEIREISGD-QYVNEDDLDKMHYLKAVMKETLRLH 370

BLAST of MS024268 vs. ExPASy TrEMBL
Match: A0A6J1E4T4 (cytochrome P450 71A22-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429935 PE=3 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 1.3e-57
Identity = 120/179 (67.04%), Postives = 144/179 (80.45%), Query Frame = 0

Query: 147 REAVAGEDGRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLI 206
           R+  AGEDG  K R LL + ++LLGSF +RD IPWLGWIDGISG+DAK KRVAKELD  +
Sbjct: 196 RKFDAGEDGM-KLRSLLLELLELLGSFGIRDFIPWLGWIDGISGLDAKGKRVAKELDVFL 255

Query: 207 DRVIQDHVHPNYNPEE--QSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTT 266
           DRVIQD VH     E   + +DLVDVLL +QR +SIGFPLEM SIKALILDMF  GT+TT
Sbjct: 256 DRVIQDRVHSENRDENSGKHKDLVDVLLWVQRNNSIGFPLEMDSIKALILDMFAAGTETT 315

Query: 267 YTVLEWAMSELLRHPEAMEKLQGEVKEVAGEKE--YVIEDDLEKMHYLKAVIKETLRLH 322
            TV+EW MSELL+HPEAM+KL+ E++E++G+ +  YV EDDL+KMHYLKAV+KETLRLH
Sbjct: 316 STVMEWTMSELLKHPEAMKKLKSEIREISGDHKAYYVNEDDLDKMHYLKAVLKETLRLH 373

BLAST of MS024268 vs. TAIR 10
Match: AT3G48290.1 (cytochrome P450, family 71, subfamily A, polypeptide 24 )

HSP 1 Score: 172.6 bits (436), Expect = 5.4e-43
Identity = 84/167 (50.30%), Postives = 118/167 (70.66%), Query Frame = 0

Query: 155 GRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHV 214
           G+  F+DL+ +  +LLG F V   + WL WID I G+D +  +++ +LD+ ++RV+QDHV
Sbjct: 193 GKTDFKDLMKRLTRLLGEFSVGSYVSWLAWIDWIRGLDGQLIKISNDLDEFLERVVQDHV 252

Query: 215 HPNYNPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWAMSE 274
               + +    D VD LL+I+R+ S+GF ++  SIKA+ILD+F G  DTTYT+LEWAM+E
Sbjct: 253 ----DGDGHKNDFVDFLLTIEREKSVGFEIDRLSIKAIILDVFVGDMDTTYTLLEWAMTE 312

Query: 275 LLRHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
           LL H E +++LQ EV+ V  +K  V EDDL+ M YLKAVIKETLRLH
Sbjct: 313 LLCHHECLDRLQEEVRMVCKDKSGVSEDDLQDMKYLKAVIKETLRLH 355

BLAST of MS024268 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 171.0 bits (432), Expect = 1.6e-42
Identity = 81/163 (49.69%), Postives = 115/163 (70.55%), Query Frame = 0

Query: 159 FRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNY 218
           F++L+ +  KLLG F V   +PWL WID ISG+D + K+   +LD+ +++V+QDH     
Sbjct: 196 FKELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDLDEFLEKVVQDH----E 255

Query: 219 NPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWAMSELLRH 278
           + + Q  D VDVLL IQR+ S+GF ++  SIKA+ILD+  GGTDT+Y ++EWAM+ELL  
Sbjct: 256 DGDAQRTDFVDVLLRIQREKSVGFEIDRLSIKAIILDVVVGGTDTSYALMEWAMTELLHR 315

Query: 279 PEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
           PE + +LQ EV+ +      V EDD++ M+YLKAVIKET+RLH
Sbjct: 316 PECLNRLQEEVRTICKGNSSVSEDDIKDMNYLKAVIKETMRLH 354

BLAST of MS024268 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 169.9 bits (429), Expect = 3.5e-42
Identity = 79/167 (47.31%), Postives = 117/167 (70.06%), Query Frame = 0

Query: 155 GRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHV 214
           G   F++L+ +  KLLG+F V   +PWL WID I G+D + ++ A ++D   +RV+QDHV
Sbjct: 191 GETDFKELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTANDVDKFFERVVQDHV 250

Query: 215 HPNYNPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWAMSE 274
             N        D VDVLL+IQR  ++GF +   SIKA+++++F GGTDT+ T++EWAM+E
Sbjct: 251 DGN----RDMTDFVDVLLAIQRDKTVGFEINRVSIKAIVMNVFVGGTDTSSTLMEWAMTE 310

Query: 275 LLRHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
           LLRHP+ +++LQ EV+ +  +K  V E++++ M YLKAVIKE LRLH
Sbjct: 311 LLRHPKCLKRLQEEVRTICKDKSSVSEEEIQNMSYLKAVIKEALRLH 353

BLAST of MS024268 vs. TAIR 10
Match: AT3G48280.1 (cytochrome P450, family 71, subfamily A, polypeptide 25 )

HSP 1 Score: 167.2 bits (422), Expect = 2.3e-41
Identity = 79/167 (47.31%), Postives = 113/167 (67.66%), Query Frame = 0

Query: 155 GRNKFRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHV 214
           G   F+ L  +  +LLG+F +   +PWL W+D I G DA+  ++ K+LDD  ++V+QDH 
Sbjct: 190 GETDFKKLTDRLSELLGTFSIGSFVPWLAWVDWIRGWDAQLDKMGKDLDDFFEKVVQDH- 249

Query: 215 HPNYNPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWAMSE 274
                      DL+D LL ++R+ S GF +E  SIKA+ LD+F GG+DT++T+LEWAM+E
Sbjct: 250 --EDGDRRDGTDLIDALLRVKREKSPGFEIERVSIKAITLDVFVGGSDTSFTLLEWAMTE 309

Query: 275 LLRHPEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
           LLRHP+++ +LQ EV+ +   K  V EDD++ M YLKAVIKE LRLH
Sbjct: 310 LLRHPKSLNRLQEEVRTICKGKSRVSEDDIQGMKYLKAVIKEALRLH 353

BLAST of MS024268 vs. TAIR 10
Match: AT3G48300.1 (cytochrome P450, family 71, subfamily A, polypeptide 23 )

HSP 1 Score: 164.9 bits (416), Expect = 1.1e-40
Identity = 76/163 (46.63%), Postives = 113/163 (69.33%), Query Frame = 0

Query: 159 FRDLLPQFMKLLGSFIVRDLIPWLGWIDGISGMDAKAKRVAKELDDLIDRVIQDHVHPNY 218
           F++L+ + M+ LG+F +   +PWL W D +SG++A+ ++ A + D L++R++QDH     
Sbjct: 193 FKELIDRLMRQLGTFTIGSYVPWLAWTDWVSGLEARLEKTANDFDKLLERIVQDH----E 252

Query: 219 NPEEQSRDLVDVLLSIQRQDSIGFPLEMKSIKALILDMFGGGTDTTYTVLEWAMSELLRH 278
           + +    D VDVLL+ QR  S GF ++  SIKA++LD F GGTDT+ T++EW M+ELLRH
Sbjct: 253 DGDGDKTDFVDVLLAAQRDKSFGFDIDRLSIKAIVLDAFVGGTDTSSTLVEWEMTELLRH 312

Query: 279 PEAMEKLQGEVKEVAGEKEYVIEDDLEKMHYLKAVIKETLRLH 322
           P  ++KLQ EV+ +   K  V EDD++ M YLKAV+KE LRLH
Sbjct: 313 PTCLKKLQEEVRTICKGKSSVSEDDIQGMEYLKAVVKEALRLH 351

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134640.12.8e-7887.93cytochrome P450 71A24-like [Momordica charantia][more]
XP_022134746.14.5e-6071.19cytochrome P450 71A26-like [Momordica charantia][more]
XP_022933473.11.0e-5971.18cytochrome P450 71A3-like [Cucurbita moschata][more]
XP_022987235.17.2e-5872.29cytochrome P450 71A25-like [Cucurbita maxima][more]
XP_023515571.17.2e-5868.16cytochrome P450 71A24-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A068Q6L27.8e-4742.55Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1[more]
P371192.1e-4457.41Cytochrome P450 71A3 (Fragment) OS=Solanum melongena OX=4111 GN=CYP71A3 PE=2 SV=... [more]
P244654.0e-4350.58Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
Q427161.5e-4240.43Cytochrome P450 71A8 OS=Mentha piperita OX=34256 GN=CYP71A8 PE=3 SV=1[more]
Q9STK97.6e-4250.30Cytochrome P450 71A24 OS=Arabidopsis thaliana OX=3702 GN=CYP71A24 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1BYV51.4e-7887.93cytochrome P450 71A24-like OS=Momordica charantia OX=3673 GN=LOC111006861 PE=3 S... [more]
A0A6J1BYN72.2e-6071.19cytochrome P450 71A26-like OS=Momordica charantia OX=3673 GN=LOC111006942 PE=3 S... [more]
A0A6J1F4U44.9e-6071.18cytochrome P450 71A3-like OS=Cucurbita moschata OX=3662 GN=LOC111440879 PE=3 SV=... [more]
A0A6J1JG963.5e-5872.29cytochrome P450 71A25-like OS=Cucurbita maxima OX=3661 GN=LOC111484850 PE=3 SV=1[more]
A0A6J1E4T41.3e-5767.04cytochrome P450 71A22-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429... [more]
Match NameE-valueIdentityDescription
AT3G48290.15.4e-4350.30cytochrome P450, family 71, subfamily A, polypeptide 24 [more]
AT3G48310.11.6e-4249.69cytochrome P450, family 71, subfamily A, polypeptide 22 [more]
AT3G48270.13.5e-4247.31cytochrome P450, family 71, subfamily A, polypeptide 26 [more]
AT3G48280.12.3e-4147.31cytochrome P450, family 71, subfamily A, polypeptide 25 [more]
AT3G48300.11.1e-4046.63cytochrome P450, family 71, subfamily A, polypeptide 23 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 248..265
score: 34.55
coord: 311..326
score: 27.84
coord: 268..294
score: 36.78
IPR001128Cytochrome P450PRINTSPR00385P450coord: 312..323
score: 46.52
coord: 259..276
score: 38.21
IPR001128Cytochrome P450PFAMPF00067p450coord: 177..324
e-value: 1.7E-33
score: 116.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 144..325
e-value: 7.0E-39
score: 135.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 153..322
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..83
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..38
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 127..150
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 54..81
NoneNo IPR availablePANTHERPTHR47955CYTOCHROME P450 FAMILY 71 PROTEINcoord: 150..323

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS024268.1MS024268.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen