MS023999 (gene) Bitter gourd (TR) v1

Overview


Name	MS023999
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pyruvate kinase
Location	scaffold44: 1795501 .. 1800650 (+)
RNA-Seq Expression	MS023999
Synteny	MS023999

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCAGGTGGTGGCTACAAGGTCGATTTGCAGCACATTCCTGTGCCCTAGCTCGGGATCTGTGCAGGACCGGGCGGACAAGGCTCGGTCTTTTGGTTTTGGCTCGAAAGTGCTGGCGCACGAGAAGAAGAGTTGGAACTTTGTTTCCAGGAGAAGTCTAGTCACAGCGAAAAGAGCTGCTCAAGCTGCAGTTGTTCCGGTATCACCTGAGGATGTTCAGAAGGTTTGTGATCTTTTGAGCTGTTAACTTTTTTAGGAAAATGCTTTTATTTGCTTGTTACGAAAAGATTGAGAAAACAAGGGGAGCTGGAGTCATGGAATTCGAATATCTGTAAGAATTAAGGAGCTGTATGACAGGCACGTAGCATTCAGGGAGCTAAATGGTGTATTTTATTATATTGCTTCAAGCTGTACGGATACATAATTGGGTATAATTCCTGCATTTGGATGCGTAGAGTTTTGTACGGCTGGAAATTCAGAATGCATATTTCTGTTGCTGAGAAAGTAAATCATCAGAAAATGGGATTTTCCCCTTATATATAGTAATATAACATGAATTAACATATATATATGAATGGTTGCCGAGAATCCTCTTCTAGTTTTCTTATTTTGTTTACCAATGGAAATGGATACTGTGTAGTCTTCATTGGCTGTAAATGCTAGTGATTAGTGAATTTAAAATTTCTCGTCTTAATATGTTTCTAAATCTTGCTTATCAGGCAGATGAGCAATTTGAACATTTACGAGCAGTTCAACAACTTGGTGATACACCGGTTGGAATGTGGTCCAAACCAACAGTTAGACGTAAGACAAAGATTGTGTGCACGATTGGTCCTTCGACTAACACGAAGGAAATGATTTGGAAACTGGCCGAGGCTGGAATGAATGTTGCTCGATTGAATATGTCTCATGGAGACCATGCATCTCATCAGAAAGTTATAGATTTAGTCAGAGAATACAATGCCCAATCACAAGACAACTGCATTGCGATCATGCTCGACACCAAGGCAAGTTCATTTTCATATTTTCATATGTTCTGTACTTTCATTAACCTAGCAACAAATGTTTTTCAATGTAACAAGTTCAATTTTCCTGGAATTTACAAATATTGTATTATCCATCTTTACTCTGCCAGAGGATAAGGATGCACCACCAAGGTTAAAAGAATCTTCTAAAAACAAGTCTACAGTTTCAACTTTACAATGCATTTTGTTTGTTGACTGCTGAAATGCTAATGTCATACATACCCTTTTGTTTTATTCACATTTTTGTCTCTTTTAAGCCTTGATTTTTTCCTTGTGCTGAATATTGACAAACGGTAGACCGATTCATTATGTAACCAATTATGTTTTCTAATTTTTTTTTTTTTTTTGGTAAATTTGGTATAACCATTACGTCATTCAGGGTTTTTCTTTTTAATCATGTATTTCATTTTGCAGGGTCCTGAGGTTAGGAGTGGTGACCTACCACAACCAATATTGTTAGAGACTGGACAGGAATTCACATTTTCCATACGGAGAGGTGTTAGTACTCCAGATTCTGTCAGCGTAAACTATGACGATTTTGTTAATGATGTAGAAGTTGGGGACATGCTTCTTGTTGATGGTATGCCGCATTCTTTTATTTGATTCTCCTTTCTGTGTGTATATATCCTAATGATTAAAATCTTGGTTTACATTCCATGCGGTGAAAAGTCTTGGCATTGTAGCATATGATTTTTGACGTTGTAGTATTATCTTGATATACTTATGTTGCTTCATTTTTCTTTTGGGGACATGTTAATTTCATTTAATGACTGTATAAAGATACCCTTATATTTGTATTTTAATGCTAATTTCACTTTTTTTTTTCTTTCTCATACGTTGTAAACTCTCCTTACTATTTTAAATGAAATTTTCATTTTACATCTTAAGATTATTACTACTTGAAATTTATATCAAGAAGAGATAAAGCTTTTTTGTCATCATCTCATGCATTGCAGCAACATTGACTCTTGAAATCTCGTTATAGGTGGAATGATGTCATTGATGGTGAAGTCTAAGACAGAGGATTCGGTGAAGTGTGAAGTTGTTGATGGAGGAGAGCTCAAGTCCAGGCGACATTTAAATGTTCGAGGGAAAAGTGCAACACTGCCTTCCATTACTGGTTCGATTTTGAATTAGCATTTCCTACAAATTTCACTTTTTATTGAAGTTTCCTTTTTAGTAGTGGACAACGTGGGTGTTCTGGATTTTTTTTTTTTTAATATTTTTCTGCTGTGACCCAACAAATATGTTGTACTGCTTTATGTATTTTTGCAGAGAAGGACTGGGATGACATAAAATTTGGAGTTGACAACAAAGTTGATTTTTATGCCGTCTCTTTCGTTAAAGATGCACAAGTGGTTCATGAGTTGAAGAATTATCTTAAAAGTAAACAACTGCATATCTATGTTGTGTTTATAAAGTCGATTTGGATGCTAATAAATTGTCTGACTCTGCAGGCTGTGATGCTGACATTCATATAATTGTAAAAATAGAAAGCGCAGACTCCATACCAAATTTGCATTCAATTATTACAGCATCTGATGGGGTAATTATTAGATTTCTTTACAGAATCGGACTGGATCTTCATGAGTTAATGTTGAATTCAGTTCAAGCTTTTTCAATTTGGTTGATACTTTGGGGCTTGATTGCTAATTTAAGGGGCAATCCCTTTTATCATTCTAGGCAATGGTTGCAAGAGGTGATCTTGGTGCGGAACTCCCTATTGAAGAGGTTCCGCTTCTGCAGGTAGTTAATGTTTCCCAATGAAAGAGAGGCAGCGGAGATTATTTCCCCTTGTGCCTTTTTTTCACTCTTAGAAGAGATGTTTAGTGTTTTATTCGCCAAACTTCCTAAACATGCTTGATTGAAATGAAGGAGGAGATAATAAACATGTGTCGTGGCATGGGGAAGGCTGTCATTGTGGCAACTAACATGCTTGAGAGTATGATTGTTCATCCAACACCAACCAGAGCAGAAGTATCTGACATTGCTATTGCTGTTAGAGAGGGTGCTGATGCAGTCATGCTTTCTGGAGAAACTGCTCACGGAAAGTGAGTTATTATTTTGAGTTCCTTAGTACATTAGTTAGAACATGTGATTGGCCCATATGTTTGGGGCATATTATATTGTTTTGTGCACTTAGGAATTTTTGGGTCAGATTATGTTGTTTCTTTAGATCTTGCGATGGTTCCCAAGATTAAGTGTTCTACGGTGGGTACAGAATGATATGATTCTGCAAAACAATAATTCATATTGATTAATTGGTTTTTAAAAGCTATAAGGCATGATACGTGCAGATATTGAGCGCATTACGAAATCAAATAGGAATAAATGAATTTGAGTAAATAAACATACTTCAGCTGTCTTCTTTTGTTGCCTTCGAGTCTTTTTGGTTATAAATAAGTATGTTCTTGCATTTTGAACACCGACTTTGCCCATTTACAGATTCCCATTAAAAGCTGTGAAAGTAATGCATACAGTTGCTTTACGGACTGAAGCGACCATAGCCGGTGGTCAAATGCCATTGAATCTCGGCCAAACATTCAAGGTTGTTTAAAAATTAGTAATTATTTAATACTTCAGTAGGAACATAATTTTGTGCTCTTTCCACTCTTCTGTTTTTTCATTAGTTCTATTTCTCCATGACATTGACTTGGTTACTTTTCTTTATGTAGAACCATATGAGCGAGATGTTTGCTTATCATGCAACAATGATGTCCAACACTCTTGGAACCTCAATAGTTGTCTTCACTAGAACTGGTTTTATGGCTATCCTCCTTAGCCATTATCGACCATCTGGCACAACATTTGCCTTTACAAACGAGTGAGTTGCTTGTGTACTTGAAAGCCAATCATATATCATGTGACCTAGATACGATTGCATTCAAGATTCTGGTTCTTTTCCTTTTTGTTGTTTTTTATTTTATTGTCTGACAGAACCAAAACTTGTTTTCATCGAACTGCTTTATTATTCTCTAGTCTCCTATTTCTTTCCTCCACATTTATGTGTCAATACTTCATGATCTTGATAGTTCTTTCTATTGTGCTCAAAGTATTGGCATAAGGTGCTCTTATTGATGTAGGCATCACATACAAAGATACAAAAATTCGAGTCCATTATTGATGTAAATGTTTGCCATTTCAATATTGGTCCACTGAGTTAATCTGCATCTAGTAGGATCTTATAGAAAAGGCTAGTATCATGGATGAAAATGCTTTCTTCCTGTATGTGGCCAAAGGAGTAATTCATGAGTAGGAAATATAAAATTTAGTCGGGTTCTTGGATTTTAGTTATGATAGTATCACTATCGTTAAATACCCTACTGTGCCATGCAGTAAAACAATACAACAGAGGTTGGCTTTATATCAGGGAGTGTGCCCCATATACATGCAGTTCTCTGATGATGCTGAGAAGACCTTTGCTGATGCCTTATCTCTGCTTCAGGTATGGGTCCTATCTGCAATTTGTTTTCTCTTTTCGTTTCTTTTATCTGGAGGGAACCTTTTTAATGCCTTCTCTGAAATAGTACGAATACTATGTTTCGTTTCCATTTAGCAATGATTTTATTGCATAATATGGCAGGTTATTATAACTTAGAAGGTGCAGGGTTGTAGTTTAAATTATTTATTATTCTTGTCCTCGTGTATTCTCCAGTAATATGGTGATGCATATGGTCATTGTTCACTTAGATGACCATTTAAGAAAGTTTGATCTTCAACTTGGAAATCTTCATGGAAGCTTTTTCAAATTCTATCCCAACAGTTTTACCACATCTCTAACTAATCCCAAAAATACCAATCAGTGGGAAGAACCCCAAATAAGAAAGGTACTAGTAACTTGAGAGACATTGTCCTTGAAATCAAAACTGAGTGGATCTGGACTGAACTTTCCCCTGATATTGCCATCGGCAGATGAAAGAGATGCGATCTCCGTACTATAATTCCACTTGATCTGCACTTAAATTTTCTGGTGAGCATGTACCTTGTTCTGACTCATTAAGTTTGTTGTGTACAGAGGCAAGGAATGGTGAAGGAAGGAGAAGAGGTAGCACTCCTTCAGAGTGGCAGACAACCCATATGGCGGTTCCCATCTACTCACAATATTCAAGTGCGTAAAGTGTAG

mRNA sequence

ATGTCTCAGGTGGTGGCTACAAGGTCGATTTGCAGCACATTCCTGTGCCCTAGCTCGGGATCTGTGCAGGACCGGGCGGACAAGGCTCGGTCTTTTGGTTTTGGCTCGAAAGTGCTGGCGCACGAGAAGAAGAGTTGGAACTTTGTTTCCAGGAGAAGTCTAGTCACAGCGAAAAGAGCTGCTCAAGCTGCAGTTGTTCCGGTATCACCTGAGGATGTTCAGAAGGCAGATGAGCAATTTGAACATTTACGAGCAGTTCAACAACTTGGTGATACACCGGTTGGAATGTGGTCCAAACCAACAGTTAGACGTAAGACAAAGATTGTGTGCACGATTGGTCCTTCGACTAACACGAAGGAAATGATTTGGAAACTGGCCGAGGCTGGAATGAATGTTGCTCGATTGAATATGTCTCATGGAGACCATGCATCTCATCAGAAAGTTATAGATTTAGTCAGAGAATACAATGCCCAATCACAAGACAACTGCATTGCGATCATGCTCGACACCAAGGCAAGTTCATTTTCATATTTTCATATGTTCTGTACTTTCATTAACCTAGCAACAAATGGTCCTGAGGTTAGGAGTGGTGACCTACCACAACCAATATTGTTAGAGACTGGACAGGAATTCACATTTTCCATACGGAGAGGTGTTAGTACTCCAGATTCTGTCAGCGTAAACTATGACGATTTTGTTAATGATGTAGAAGTTGGGGACATGCTTCTTGTTGATGGTGGAATGATGTCATTGATGGTGAAGTCTAAGACAGAGGATTCGGTGAAGTGTGAAGTTGTTGATGGAGGAGAGCTCAAGTCCAGGCGACATTTAAATGTTCGAGGGAAAAGTGCAACACTGCCTTCCATTACTGAGAAGGACTGGGATGACATAAAATTTGGAGTTGACAACAAAGTTGATTTTTATGCCGTCTCTTTCGTTAAAGATGCACAAGTGGCAATGGTTGCAAGAGGTGATCTTGGTGCGGAACTCCCTATTGAAGAGGTTCCGCTTCTGCAGGAGGAGATAATAAACATGTGTCGTGGCATGGGGAAGGCTGTCATTGTGGCAACTAACATGCTTGAGAGTATGATTGTTCATCCAACACCAACCAGAGCAGAAGTATCTGACATTGCTATTGCTGTTAGAGAGGGTGCTGATGCAGTCATGCTTTCTGGAGAAACTGCTCACGGAAAATTCCCATTAAAAGCTGTGAAAGTAATGCATACAGTTGCTTTACGGACTGAAGCGACCATAGCCGGTGGTCAAATGCCATTGAATCTCGGCCAAACATTCAAGAACCATATGAGCGAGATGTTTGCTTATCATGCAACAATGATGTCCAACACTCTTGGAACCTCAATAGTTGTCTTCACTAGAACTGGTTTTATGGCTATCCTCCTTAGCCATTATCGACCATCTGGCACAACATTTGCCTTTACAAACGATAAAACAATACAACAGAGGTTGGCTTTATATCAGGGAGTGTGCCCCATATACATGCAGTTCTCTGATGATGCTGAGAAGACCTTTGCTGATGCCTTATCTCTGCTTCAGAGGCAAGGAATGGTGAAGGAAGGAGAAGAGGTAGCACTCCTTCAGAGTGGCAGACAACCCATATGGCGGTTCCCATCTACTCACAATATTCAAGTGCGTAAAGTGTAG

Coding sequence (CDS)

ATGTCTCAGGTGGTGGCTACAAGGTCGATTTGCAGCACATTCCTGTGCCCTAGCTCGGGATCTGTGCAGGACCGGGCGGACAAGGCTCGGTCTTTTGGTTTTGGCTCGAAAGTGCTGGCGCACGAGAAGAAGAGTTGGAACTTTGTTTCCAGGAGAAGTCTAGTCACAGCGAAAAGAGCTGCTCAAGCTGCAGTTGTTCCGGTATCACCTGAGGATGTTCAGAAGGCAGATGAGCAATTTGAACATTTACGAGCAGTTCAACAACTTGGTGATACACCGGTTGGAATGTGGTCCAAACCAACAGTTAGACGTAAGACAAAGATTGTGTGCACGATTGGTCCTTCGACTAACACGAAGGAAATGATTTGGAAACTGGCCGAGGCTGGAATGAATGTTGCTCGATTGAATATGTCTCATGGAGACCATGCATCTCATCAGAAAGTTATAGATTTAGTCAGAGAATACAATGCCCAATCACAAGACAACTGCATTGCGATCATGCTCGACACCAAGGCAAGTTCATTTTCATATTTTCATATGTTCTGTACTTTCATTAACCTAGCAACAAATGGTCCTGAGGTTAGGAGTGGTGACCTACCACAACCAATATTGTTAGAGACTGGACAGGAATTCACATTTTCCATACGGAGAGGTGTTAGTACTCCAGATTCTGTCAGCGTAAACTATGACGATTTTGTTAATGATGTAGAAGTTGGGGACATGCTTCTTGTTGATGGTGGAATGATGTCATTGATGGTGAAGTCTAAGACAGAGGATTCGGTGAAGTGTGAAGTTGTTGATGGAGGAGAGCTCAAGTCCAGGCGACATTTAAATGTTCGAGGGAAAAGTGCAACACTGCCTTCCATTACTGAGAAGGACTGGGATGACATAAAATTTGGAGTTGACAACAAAGTTGATTTTTATGCCGTCTCTTTCGTTAAAGATGCACAAGTGGCAATGGTTGCAAGAGGTGATCTTGGTGCGGAACTCCCTATTGAAGAGGTTCCGCTTCTGCAGGAGGAGATAATAAACATGTGTCGTGGCATGGGGAAGGCTGTCATTGTGGCAACTAACATGCTTGAGAGTATGATTGTTCATCCAACACCAACCAGAGCAGAAGTATCTGACATTGCTATTGCTGTTAGAGAGGGTGCTGATGCAGTCATGCTTTCTGGAGAAACTGCTCACGGAAAATTCCCATTAAAAGCTGTGAAAGTAATGCATACAGTTGCTTTACGGACTGAAGCGACCATAGCCGGTGGTCAAATGCCATTGAATCTCGGCCAAACATTCAAGAACCATATGAGCGAGATGTTTGCTTATCATGCAACAATGATGTCCAACACTCTTGGAACCTCAATAGTTGTCTTCACTAGAACTGGTTTTATGGCTATCCTCCTTAGCCATTATCGACCATCTGGCACAACATTTGCCTTTACAAACGATAAAACAATACAACAGAGGTTGGCTTTATATCAGGGAGTGTGCCCCATATACATGCAGTTCTCTGATGATGCTGAGAAGACCTTTGCTGATGCCTTATCTCTGCTTCAGAGGCAAGGAATGGTGAAGGAAGGAGAAGAGGTAGCACTCCTTCAGAGTGGCAGACAACCCATATGGCGGTTCCCATCTACTCACAATATTCAAGTGCGTAAAGTGTAG

Protein sequence

MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRAAQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKASSFSYFHMFCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVAMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV

Homology

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BLAST of MS023999 vs. NCBI nr
Match: XP_022140989.1 (plastidial pyruvate kinase 2 [Momordica charantia])

HSP 1 Score: 1022.3 bits (2642), Expect = 1.5e-294
Identity = 534/592 (90.20%), Postives = 534/592 (90.20%), Query Frame = 0


Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA
Sbjct: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKASSFSYFHM 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK         
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK--------- 180

Query: 181 FCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD 240
                     GPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD
Sbjct: 181 ----------GPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD 240

Query: 241 MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300
           MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG
Sbjct: 241 MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300

Query: 301 VDNKVDFYAVSFVKDAQV---------------------------------------AMV 360
           VDNKVDFYAVSFVKDAQV                                       AMV
Sbjct: 301 VDNKVDFYAVSFVKDAQVVHELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMV 360

Query: 361 ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420
           ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV
Sbjct: 361 ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420

Query: 421 REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY 480
           REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY
Sbjct: 421 REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY 480

Query: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ 540
           HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ
Sbjct: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ 540

Query: 541 FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 554
           FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

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BLAST of MS023999 vs. NCBI nr
Match: XP_038874991.1 (plastidial pyruvate kinase 2 [Benincasa hispida])

HSP 1 Score: 981.1 bits (2535), Expect = 4.0e-282
Identity = 509/592 (85.98%), Postives = 522/592 (88.18%), Query Frame = 0


Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQ+R D+AR FG GSKV AHEKKSWNFV RRSL+T+KRA
Sbjct: 1   MSQVVATRSINSTFLCPSSGSVQERVDRARLFGIGSKVFAHEKKSWNFVYRRSLITSKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQA VVPVSPEDVQKADEQFEH+RAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHIRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKASSFSYFHM 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK         
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK--------- 180

Query: 181 FCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD 240
                     GPEVRSGDLPQPILLE+GQEFTF+IRRGVST DSVSVNYDDFVNDVEVGD
Sbjct: 181 ----------GPEVRSGDLPQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGD 240

Query: 241 MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300
           MLLVDGGMMSLMVKSKTEDSVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG
Sbjct: 241 MLLVDGGMMSLMVKSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300

Query: 301 VDNKVDFYAVSFVKDAQV---------------------------------------AMV 360
           VDNKVDFYAVSFVKDAQV                                       AMV
Sbjct: 301 VDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMV 360

Query: 361 ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420
           ARGDLGAELPIEEVPLLQEEIIN+CR MGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV
Sbjct: 361 ARGDLGAELPIEEVPLLQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420

Query: 421 REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY 480
           REGADA+MLSGETAHGKFPLKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAY
Sbjct: 421 REGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAY 480

Query: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ 540
           HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQ
Sbjct: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQ 540

Query: 541 FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 554
           FSDDAE+TF DALS+LQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 FSDDAEQTFTDALSMLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

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BLAST of MS023999 vs. NCBI nr
Match: XP_008439317.1 (PREDICTED: plastidial pyruvate kinase 2 [Cucumis melo])

HSP 1 Score: 979.9 bits (2532), Expect = 8.8e-282
Identity = 509/592 (85.98%), Postives = 523/592 (88.34%), Query Frame = 0


Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQ+R D+ARSFG GSKVLAHEKKSWN   RRSL+T+KRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKASSFSYFHM 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK         
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK--------- 180

Query: 181 FCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD 240
                     GPEVRSGDLPQPILLETGQEFTF+IRRGVST D+VSVNYDDFVNDVEVGD
Sbjct: 181 ----------GPEVRSGDLPQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGD 240

Query: 241 MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300
           MLLVDGGMMSLMVKSKTEDSVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG
Sbjct: 241 MLLVDGGMMSLMVKSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300

Query: 301 VDNKVDFYAVSFVKDAQV---------------------------------------AMV 360
           VDNKVDFYAVSFVKDAQV                                       AMV
Sbjct: 301 VDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMV 360

Query: 361 ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420
           ARGDLGAELPIEEVPLLQEEIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV
Sbjct: 361 ARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420

Query: 421 REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY 480
           REGADA+MLSGETAHGKFPLKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAY
Sbjct: 421 REGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAY 480

Query: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ 540
           HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQ
Sbjct: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQ 540

Query: 541 FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 554
           FS+DAE+TF DAL++LQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 FSEDAEQTFTDALTMLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

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BLAST of MS023999 vs. NCBI nr
Match: TYK23021.1 (plastidial pyruvate kinase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 978.4 bits (2528), Expect = 2.6e-281
Identity = 508/592 (85.81%), Postives = 522/592 (88.18%), Query Frame = 0


Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQ+R D+ARSFG GSKVLAHEKKSWN   RRSL+T+KRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKASSFSYFHM 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK         
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK--------- 180

Query: 181 FCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD 240
                     GPEVRSGDLPQPILLETGQEFTF+IRRGVST D+VSVNYDDFVNDVEVGD
Sbjct: 181 ----------GPEVRSGDLPQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGD 240

Query: 241 MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300
           MLLVDGGMMSLMVKSKTEDSVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG
Sbjct: 241 MLLVDGGMMSLMVKSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300

Query: 301 VDNKVDFYAVSFVKDAQV---------------------------------------AMV 360
           VDNKVDFYAVSFVKDAQV                                       AMV
Sbjct: 301 VDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMV 360

Query: 361 ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420
           ARGDLGAELPIEEVPLLQEEIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV
Sbjct: 361 ARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420

Query: 421 REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY 480
           REGADA+MLSGETAHGKFP KAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAY
Sbjct: 421 REGADAIMLSGETAHGKFPFKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAY 480

Query: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ 540
           HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQ
Sbjct: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQ 540

Query: 541 FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 554
           FS+DAE+TF DAL++LQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 FSEDAEQTFTDALTMLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

[Close]

BLAST of MS023999 vs. NCBI nr
Match: KAG7014734.1 (Plastidial pyruvate kinase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 977.6 bits (2526), Expect = 4.4e-281
Identity = 510/613 (83.20%), Postives = 529/613 (86.30%), Query Frame = 0


Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQDR D+ARSFG GSK+L HEKKSWNF  RRSL+T+K A
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQDRVDRARSFGIGSKLLGHEKKSWNFAYRRSLITSKGA 60

Query: 61  AQAAVVPVSPEDVQK---------------------ADEQFEHLRAVQQLGDTPVGMWSK 120
            QA VVPVSPEDVQK                     ADEQFEHLRAVQQLGDTPVGMWSK
Sbjct: 61  TQAEVVPVSPEDVQKPSLAAKFQFSRLNTILNLTYQADEQFEHLRAVQQLGDTPVGMWSK 120

Query: 121 PTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQS 180
           PTVRRKTKIVCTIGPST+TKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQS
Sbjct: 121 PTVRRKTKIVCTIGPSTDTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQS 180

Query: 181 QDNCIAIMLDTKASSFSYFHMFCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGV 240
           QDNCIAIMLDTKAS       +  FIN A  GPEVRSGDLPQPILLE+GQEFTF+IRRGV
Sbjct: 181 QDNCIAIMLDTKASYLYPHIQYIHFINPAITGPEVRSGDLPQPILLESGQEFTFTIRRGV 240

Query: 241 STPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNV 300
           ST DSVSVNYDDFVNDVEVGDM+LVDGGMMSLMVKSKTE+SVKCEVVDGGELKSRRHLNV
Sbjct: 241 STADSVSVNYDDFVNDVEVGDMILVDGGMMSLMVKSKTENSVKCEVVDGGELKSRRHLNV 300

Query: 301 RGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQV--------------------- 360
           RGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQV                     
Sbjct: 301 RGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKI 360

Query: 361 ------------------AMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNML 420
                             AMVARGDLGAELPIEEVPLLQEEIIN+CRGMGKAVIVATNML
Sbjct: 361 ESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNML 420

Query: 421 ESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAG 480
           ESMIVHPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFPLKAVKVMHTVALRTEATI G
Sbjct: 421 ESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEG 480

Query: 481 GQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFT 540
           G+MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFT
Sbjct: 481 GRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFT 540

Query: 541 NDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIW 554
           NDK IQQRLALYQGVCP+YMQFSDDAE+TF+DALS+LQRQGMVKEGEEVAL+QSG+QPIW
Sbjct: 541 NDKRIQQRLALYQGVCPLYMQFSDDAEQTFSDALSMLQRQGMVKEGEEVALVQSGKQPIW 600

[Close]

BLAST of MS023999 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 8.1e-238
Identity = 437/598 (73.08%), Postives = 478/598 (79.93%), Query Frame = 0


Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADK-ARSFGFGSKVLAHEKKSWNFV---SRRSLVT 60
           M+QVVATRSI  + L P+ GSV  R++K  +   F  KVL +E K    V   SRR + T
Sbjct: 1   MAQVVATRSIQGSMLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSVRSRRVVDT 60

Query: 61  AKRAA--QAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGP 120
             R+A  +  V+PVSPEDV   +EQ E L  +QQ GDT VGMWSKPTVRRKTKIVCT+GP
Sbjct: 61  TVRSARVETEVIPVSPEDVPNREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGP 120

Query: 121 STNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKASS 180
           STNT+EMIWKLAEAGMNVAR+NMSHGDHASH+KVIDLV+EYNAQ++DN IAIMLDTK   
Sbjct: 121 STNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTK--- 180

Query: 181 FSYFHMFCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVN 240
                           GPEVRSGDLPQPI+L+ GQEFTF+I RGVSTP  VSVNYDDFVN
Sbjct: 181 ----------------GPEVRSGDLPQPIMLDPGQEFTFTIERGVSTPSCVSVNYDDFVN 240

Query: 241 DVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDW 300
           DVE GDMLLVDGGMMS MVKSKT+DSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDW
Sbjct: 241 DVEAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDW 300

Query: 301 DDIKFGVDNKVDFYAVSFVKDAQV------------------------------------ 360
           +DIKFGV+NKVDFYAVSFVKDAQV                                    
Sbjct: 301 EDIKFGVENKVDFYAVSFVKDAQVVHELKKYLQNSGADIHVIVKIESADSIPNLHSIITA 360

Query: 361 ---AMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVS 420
              AMVARGDLGAELPIEEVP+LQEEIIN+CR MGKAVIVATNMLESMIVHPTPTRAEVS
Sbjct: 361 SDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVS 420

Query: 421 DIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHM 480
           DIAIAVREGADAVMLSGETAHGKFPLKA  VMHTVALRTEATI  G+MP NLGQ FKNHM
Sbjct: 421 DIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMPPNLGQAFKNHM 480

Query: 481 SEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGV 540
           SEMFAYHATMMSNTLGTS VVFTRTGFMAILLSHYRPSGT +AFTN+K IQQRLALYQGV
Sbjct: 481 SEMFAYHATMMSNTLGTSTVVFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGV 540

Query: 541 CPIYMQFSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 554
           CPIYM+F+DDAE+TFA+AL+ L +QGMVK+GEE+A++QSG QPIWR  STHNIQVRKV
Sbjct: 541 CPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

[Close]

BLAST of MS023999 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 3.1e-181
Identity = 332/489 (67.89%), Postives = 379/489 (77.51%), Query Frame = 0


Query: 104 RKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNC 163
           RKTKIVCTIGPST+++EMIWKLAEAGMNVARLNMSHGDHASHQ+ IDLV+EYNAQ +D  
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKV 150

Query: 164 IAIMLDTKASSFSYFHMFCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPD 223
           IAIMLDTK                   GPEV SGD+P+PILL+ GQEF FSI+RGVST D
Sbjct: 151 IAIMLDTK-------------------GPEVISGDVPKPILLKEGQEFNFSIKRGVSTED 210

Query: 224 SVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKS 283
           +VSVNYDDF+NDVE GD+LLVDGGMMSL VKSKT D VKCEV+DGGELKSRRHLNVRGKS
Sbjct: 211 TVSVNYDDFINDVEAGDILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRRHLNVRGKS 270

Query: 284 ATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQV------------------------- 343
           ATLPSITEKDWDDIKFGV+N+VDFYAVSFVKDA+V                         
Sbjct: 271 ATLPSITEKDWDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYLKSCNADIHVIVKIESAD 330

Query: 344 --------------AMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMI 403
                         AMVARGDLGAELPIEEVPLLQE+II  C+ M K VIVATNMLESMI
Sbjct: 331 SIPNLHSIISASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMI 390

Query: 404 VHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMP 463
            HPTPTRAEVSDI+IAVREGADAVMLSGETAHGK+PLKAVKVMH VALRTE+++      
Sbjct: 391 DHPTPTRAEVSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTESSLQKSTSS 450

Query: 464 LNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKT 523
            +    +K+HM EMFA+H++ M+NTL T I+VFTRTG MAI+LSH RPS T FAFTN++ 
Sbjct: 451 PSQSAAYKSHMGEMFAFHSSSMANTLSTPIIVFTRTGSMAIILSHNRPSSTVFAFTNNER 510

Query: 524 IQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPS 554
           ++QRLALY GV PIYM+FS DAE+TF+ A+ LL  + +VK+G+ V L+QSG QPIWR  S
Sbjct: 511 VKQRLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLVQSGAQPIWRRHS 560

[Close]

BLAST of MS023999 vs. ExPASy Swiss-Prot
Match: Q93Z53 (Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP3 PE=1 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 4.1e-181
Identity = 335/490 (68.37%), Postives = 377/490 (76.94%), Query Frame = 0


Query: 103 RRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDN 162
           RRKTKIVCTIGPS++++EMIWKLAEAGMNVARLNMSHGDHASHQ  IDLV+EYN+   D 
Sbjct: 98  RRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDK 157

Query: 163 CIAIMLDTKASSFSYFHMFCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTP 222
            IAIMLDTK                   GPEVRSGD+PQPI LE GQEF F+I+RGVS  
Sbjct: 158 AIAIMLDTK-------------------GPEVRSGDVPQPIFLEEGQEFNFTIKRGVSLK 217

Query: 223 DSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGK 282
           D+VSVNYDDFVNDVEVGD+LLVDGGMMSL VKSKT D VKC V+DGGEL+SRRHLNVRGK
Sbjct: 218 DTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLNVRGK 277

Query: 283 SATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQV------------------------ 342
           SATLPSIT+KDW+DIKFGVDN+VDFYAVSFVKDA+V                        
Sbjct: 278 SATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCSADISVIVKIESA 337

Query: 343 ---------------AMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESM 402
                          AMVARGDLGAELPIEEVPLLQEEII  CR + K VIVATNMLESM
Sbjct: 338 DSIKNLPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESM 397

Query: 403 IVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQM 462
           I HPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFPLKAV VMHTVALRTEA++   + 
Sbjct: 398 INHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLP-VRT 457

Query: 463 PLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDK 522
             +    +K HM +MFA+HA++M+NTL + ++VFTRTG MA+LLSHYRPS T FAFTN +
Sbjct: 458 SASRTTAYKGHMGQMFAFHASIMANTLSSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQR 517

Query: 523 TIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFP 554
            I QRLALYQGV PIYM+FSDDAE T+A +L LLQ + M+KEG+ V L+QSG QPIWR  
Sbjct: 518 RIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREE 567

[Close]

BLAST of MS023999 vs. ExPASy Swiss-Prot
Match: P55964 (Pyruvate kinase isozyme G, chloroplastic (Fragment) OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 2.6e-151
Identity = 291/437 (66.59%), Postives = 327/437 (74.83%), Query Frame = 0


Query: 156 NAQSQDNCIAIMLDTKASSFSYFHMFCTFINLATNGPEVRSGDLPQPILLETGQEFTFSI 215
           NAQS DN ++IMLDTK                   GPEVRSGD+PQP+L E GQEF  +I
Sbjct: 1   NAQSHDNVVSIMLDTK-------------------GPEVRSGDVPQPMLKE-GQEFNPTI 60

Query: 216 RRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRR 275
           RRGVST D+VSVNYDDFVNDV VGD+LLVDGGMMSL VKSKT D VKC VVDGGELKSRR
Sbjct: 61  RRGVSTQDTVSVNYDDFVNDVVVGDILLVDGGMMSLAVKSKTSDLVKCVVVDGGELKSRR 120

Query: 276 HLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQV----------------- 335
           HLNVRGKSA LPSIT+KDW DIKFGVDN+VDFYAVSFVKDA+V                 
Sbjct: 121 HLNVRGKSARLPSITDKDWGDIKFGVDNQVDFYAVSFVKDAKVVHELKEYLKRCNADIHV 180

Query: 336 ----------------------AMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVA 395
                                 AMVARGDLGAELPIEEVPLLQE+II  C  M K VIVA
Sbjct: 181 IVKIESADSIPNLHSIISASDGAMVARGDLGAELPIEEVPLLQEDIIRRCHSMQKPVIVA 240

Query: 396 TNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTEA 455
           TNMLESMI HPTPTRAEVSDIAIAVREGADAVMLSGETAHGK+PLKAV+VMHTVALRTE+
Sbjct: 241 TNMLESMINHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLKAVRVMHTVALRTES 300

Query: 456 TIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTT 515
           +      P   G  +K HM EMFA+HAT+M+NTL T I+VFTRTG MA+LLSHY+P+ T 
Sbjct: 301 SSPVNTTPPAQG-AYKGHMGEMFAFHATIMANTLNTPIIVFTRTGSMAVLLSHYQPASTI 360

Query: 516 FAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGR 554
           FAFTN++ I+QRL+LY+GV PIYM+FS DAE+TF+ AL LL  +G++ EGE V L+QSG 
Sbjct: 361 FAFTNEERIKQRLSLYRGVMPIYMEFSSDAEETFSRALQLLLNKGLLVEGEHVTLVQSGA 416

[Close]

BLAST of MS023999 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.0e-82
Identity = 200/494 (40.49%), Postives = 272/494 (55.06%), Query Frame = 0


Query: 104 RKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNC 163
           R+TK++CTIGP+T   E +  LA  GMNVARLNM HG    H+ VI  VR  N + +   
Sbjct: 117 RRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLN-EEKGFA 176

Query: 164 IAIMLDTKASSFSYFHMFCTFINLATNGPEVRSGDL--PQPILLETGQEFTFSIRR-GVS 223
           +AIM+DT+                   G E+  GDL        E G+ +TF++R    S
Sbjct: 177 VAIMMDTE-------------------GSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSS 236

Query: 224 TPD-SVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLN- 283
            P+ ++SV+YD F  DV VGD LLVDGGM+   V  K    VKC   D G L  R +L  
Sbjct: 237 RPERTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTF 296

Query: 284 ------VRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQV-------------- 343
                 VR ++A LP+I+ KDW DI FG+   VDF AVSFVK A+V              
Sbjct: 297 WRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRG 356

Query: 344 ---------------------------AMVARGDLGAELPIEEVPLLQEEIINMCRGMGK 403
                                      AMVARGDLGA++P+E+VP  Q+ I+ +CR + K
Sbjct: 357 GEIGVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNK 416

Query: 404 AVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVA 463
            VIVA+ +LESMI +PTPTRAEV+D++ AVR+ +DA+MLSGE+A G+FP KA+ V+ TV+
Sbjct: 417 PVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVS 476

Query: 464 LRTE----ATIAGGQMPLN-LGQTFKNHMSEMFAYHATMMSNTLGT-SIVVFTRTGFMAI 523
           LR E           +PL  +G +F + +SE     A  M+N LG  ++ V+T +G MA 
Sbjct: 477 LRIERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMAS 536

Query: 524 LLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQGMVKE 540
           L+S  RP    FAFT   ++++RL L  G+ P  + FSDD E       SLL+ +GM+K 
Sbjct: 537 LVSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKS 590

[Close]

BLAST of MS023999 vs. ExPASy TrEMBL
Match: A0A6J1CHB6 (Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111011509 PE=3 SV=1)

HSP 1 Score: 1022.3 bits (2642), Expect = 7.5e-295
Identity = 534/592 (90.20%), Postives = 534/592 (90.20%), Query Frame = 0


Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA
Sbjct: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKASSFSYFHM 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK         
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK--------- 180

Query: 181 FCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD 240
                     GPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD
Sbjct: 181 ----------GPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD 240

Query: 241 MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300
           MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG
Sbjct: 241 MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300

Query: 301 VDNKVDFYAVSFVKDAQV---------------------------------------AMV 360
           VDNKVDFYAVSFVKDAQV                                       AMV
Sbjct: 301 VDNKVDFYAVSFVKDAQVVHELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMV 360

Query: 361 ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420
           ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV
Sbjct: 361 ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420

Query: 421 REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY 480
           REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY
Sbjct: 421 REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY 480

Query: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ 540
           HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ
Sbjct: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ 540

Query: 541 FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 554
           FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

[Close]

BLAST of MS023999 vs. ExPASy TrEMBL
Match: A0A1S3AY45 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103484139 PE=3 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 4.3e-282
Identity = 509/592 (85.98%), Postives = 523/592 (88.34%), Query Frame = 0


Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQ+R D+ARSFG GSKVLAHEKKSWN   RRSL+T+KRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKASSFSYFHM 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK         
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK--------- 180

Query: 181 FCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD 240
                     GPEVRSGDLPQPILLETGQEFTF+IRRGVST D+VSVNYDDFVNDVEVGD
Sbjct: 181 ----------GPEVRSGDLPQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGD 240

Query: 241 MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300
           MLLVDGGMMSLMVKSKTEDSVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG
Sbjct: 241 MLLVDGGMMSLMVKSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300

Query: 301 VDNKVDFYAVSFVKDAQV---------------------------------------AMV 360
           VDNKVDFYAVSFVKDAQV                                       AMV
Sbjct: 301 VDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMV 360

Query: 361 ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420
           ARGDLGAELPIEEVPLLQEEIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV
Sbjct: 361 ARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420

Query: 421 REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY 480
           REGADA+MLSGETAHGKFPLKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAY
Sbjct: 421 REGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAY 480

Query: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ 540
           HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQ
Sbjct: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQ 540

Query: 541 FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 554
           FS+DAE+TF DAL++LQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 FSEDAEQTFTDALTMLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

[Close]

BLAST of MS023999 vs. ExPASy TrEMBL
Match: A0A5D3DH87 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G001310 PE=3 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 1.2e-281
Identity = 508/592 (85.81%), Postives = 522/592 (88.18%), Query Frame = 0


Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQ+R D+ARSFG GSKVLAHEKKSWN   RRSL+T+KRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKASSFSYFHM 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK         
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK--------- 180

Query: 181 FCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD 240
                     GPEVRSGDLPQPILLETGQEFTF+IRRGVST D+VSVNYDDFVNDVEVGD
Sbjct: 181 ----------GPEVRSGDLPQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGD 240

Query: 241 MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300
           MLLVDGGMMSLMVKSKTEDSVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG
Sbjct: 241 MLLVDGGMMSLMVKSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300

Query: 301 VDNKVDFYAVSFVKDAQV---------------------------------------AMV 360
           VDNKVDFYAVSFVKDAQV                                       AMV
Sbjct: 301 VDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMV 360

Query: 361 ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420
           ARGDLGAELPIEEVPLLQEEIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV
Sbjct: 361 ARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420

Query: 421 REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY 480
           REGADA+MLSGETAHGKFP KAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAY
Sbjct: 421 REGADAIMLSGETAHGKFPFKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAY 480

Query: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ 540
           HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQ
Sbjct: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQ 540

Query: 541 FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 554
           FS+DAE+TF DAL++LQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 FSEDAEQTFTDALTMLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

[Close]

BLAST of MS023999 vs. ExPASy TrEMBL
Match: A0A6J1E5B9 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430719 PE=3 SV=1)

HSP 1 Score: 976.1 bits (2522), Expect = 6.2e-281
Identity = 505/592 (85.30%), Postives = 522/592 (88.18%), Query Frame = 0


Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSVQDR D+ARSFG GSK+L HEKKSWNF  RRSL+T+K A
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQDRVDRARSFGIGSKLLGHEKKSWNFAYRRSLITSKGA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
            QA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPST+TKE
Sbjct: 61  TQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTDTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKASSFSYFHM 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK         
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK--------- 180

Query: 181 FCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD 240
                     GPEVRSGDLPQPILLE+GQEFTF+IRRGVST DSVSVNYDDFVNDVEVGD
Sbjct: 181 ----------GPEVRSGDLPQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGD 240

Query: 241 MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300
           M+LVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG
Sbjct: 241 MILVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300

Query: 301 VDNKVDFYAVSFVKDAQV---------------------------------------AMV 360
           VDNKVDFYAVSFVKDAQV                                       AMV
Sbjct: 301 VDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMV 360

Query: 361 ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420
           ARGDLGAELPIEEVPLLQEEIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV
Sbjct: 361 ARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420

Query: 421 REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY 480
           REGADA+MLSGETAHGKFPLKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAY
Sbjct: 421 REGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAY 480

Query: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ 540
           HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCP+YMQ
Sbjct: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPLYMQ 540

Query: 541 FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 554
           FSDDAE+TF+DALS+LQRQGMVKEGEEVAL+QSG+QPIWRFPSTHNIQVRKV
Sbjct: 541 FSDDAEQTFSDALSMLQRQGMVKEGEEVALVQSGKQPIWRFPSTHNIQVRKV 573

[Close]

BLAST of MS023999 vs. ExPASy TrEMBL
Match: A0A6J1J4F4 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482565 PE=3 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 1.8e-280
Identity = 505/592 (85.30%), Postives = 521/592 (88.01%), Query Frame = 0


Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60
           MSQVVATRSI STFLCPSSGSV DR D+ARSFG GSK+L HEKKSWNF  RRSL+T+K A
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVHDRVDRARSFGIGSKLLRHEKKSWNFAYRRSLITSKGA 60

Query: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
            QA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPST+TKE
Sbjct: 61  TQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTDTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKASSFSYFHM 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK         
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTK--------- 180

Query: 181 FCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGD 240
                     GPEVRSGDLPQPILLE+GQEFTF+IRRGVST DSVSVNYDDFVNDVEVGD
Sbjct: 181 ----------GPEVRSGDLPQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGD 240

Query: 241 MLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300
           M+LVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG
Sbjct: 241 MILVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFG 300

Query: 301 VDNKVDFYAVSFVKDAQV---------------------------------------AMV 360
           VDNKVDFYAVSFVKDAQV                                       AMV
Sbjct: 301 VDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMV 360

Query: 361 ARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420
           ARGDLGAELPIEEVPLLQEEIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV
Sbjct: 361 ARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAV 420

Query: 421 REGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAY 480
           REGADA+MLSGETAHGKFPLKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAY
Sbjct: 421 REGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAY 480

Query: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQ 540
           HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQ
Sbjct: 481 HATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQ 540

Query: 541 FSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 554
           FSDDAE+TF+DALS+LQRQGMVKEGEEVAL+QSG+QPIWRFPSTHNIQVRKV
Sbjct: 541 FSDDAEQTFSDALSMLQRQGMVKEGEEVALVQSGKQPIWRFPSTHNIQVRKV 573

[Close]

BLAST of MS023999 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 824.3 bits (2128), Expect = 5.8e-239
Identity = 437/598 (73.08%), Postives = 478/598 (79.93%), Query Frame = 0


Query: 1   MSQVVATRSICSTFLCPSSGSVQDRADK-ARSFGFGSKVLAHEKKSWNFV---SRRSLVT 60
           M+QVVATRSI  + L P+ GSV  R++K  +   F  KVL +E K    V   SRR + T
Sbjct: 1   MAQVVATRSIQGSMLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSVRSRRVVDT 60

Query: 61  AKRAA--QAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGP 120
             R+A  +  V+PVSPEDV   +EQ E L  +QQ GDT VGMWSKPTVRRKTKIVCT+GP
Sbjct: 61  TVRSARVETEVIPVSPEDVPNREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGP 120

Query: 121 STNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKASS 180
           STNT+EMIWKLAEAGMNVAR+NMSHGDHASH+KVIDLV+EYNAQ++DN IAIMLDTK   
Sbjct: 121 STNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTK--- 180

Query: 181 FSYFHMFCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVN 240
                           GPEVRSGDLPQPI+L+ GQEFTF+I RGVSTP  VSVNYDDFVN
Sbjct: 181 ----------------GPEVRSGDLPQPIMLDPGQEFTFTIERGVSTPSCVSVNYDDFVN 240

Query: 241 DVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDW 300
           DVE GDMLLVDGGMMS MVKSKT+DSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDW
Sbjct: 241 DVEAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDW 300

Query: 301 DDIKFGVDNKVDFYAVSFVKDAQV------------------------------------ 360
           +DIKFGV+NKVDFYAVSFVKDAQV                                    
Sbjct: 301 EDIKFGVENKVDFYAVSFVKDAQVVHELKKYLQNSGADIHVIVKIESADSIPNLHSIITA 360

Query: 361 ---AMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVS 420
              AMVARGDLGAELPIEEVP+LQEEIIN+CR MGKAVIVATNMLESMIVHPTPTRAEVS
Sbjct: 361 SDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVS 420

Query: 421 DIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHM 480
           DIAIAVREGADAVMLSGETAHGKFPLKA  VMHTVALRTEATI  G+MP NLGQ FKNHM
Sbjct: 421 DIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMPPNLGQAFKNHM 480

Query: 481 SEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGV 540
           SEMFAYHATMMSNTLGTS VVFTRTGFMAILLSHYRPSGT +AFTN+K IQQRLALYQGV
Sbjct: 481 SEMFAYHATMMSNTLGTSTVVFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGV 540

Query: 541 CPIYMQFSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 554
           CPIYM+F+DDAE+TFA+AL+ L +QGMVK+GEE+A++QSG QPIWR  STHNIQVRKV
Sbjct: 541 CPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

[Close]

BLAST of MS023999 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 636.0 bits (1639), Expect = 2.9e-182
Identity = 335/490 (68.37%), Postives = 377/490 (76.94%), Query Frame = 0


Query: 103 RRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDN 162
           RRKTKIVCTIGPS++++EMIWKLAEAGMNVARLNMSHGDHASHQ  IDLV+EYN+   D 
Sbjct: 98  RRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDK 157

Query: 163 CIAIMLDTKASSFSYFHMFCTFINLATNGPEVRSGDLPQPILLETGQEFTFSIRRGVSTP 222
            IAIMLDTK                   GPEVRSGD+PQPI LE GQEF F+I+RGVS  
Sbjct: 158 AIAIMLDTK-------------------GPEVRSGDVPQPIFLEEGQEFNFTIKRGVSLK 217

Query: 223 DSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGK 282
           D+VSVNYDDFVNDVEVGD+LLVDGGMMSL VKSKT D VKC V+DGGEL+SRRHLNVRGK
Sbjct: 218 DTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLNVRGK 277

Query: 283 SATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQV------------------------ 342
           SATLPSIT+KDW+DIKFGVDN+VDFYAVSFVKDA+V                        
Sbjct: 278 SATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCSADISVIVKIESA 337

Query: 343 ---------------AMVARGDLGAELPIEEVPLLQEEIINMCRGMGKAVIVATNMLESM 402
                          AMVARGDLGAELPIEEVPLLQEEII  CR + K VIVATNMLESM
Sbjct: 338 DSIKNLPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESM 397

Query: 403 IVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVALRTEATIAGGQM 462
           I HPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFPLKAV VMHTVALRTEA++   + 
Sbjct: 398 INHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLP-VRT 457

Query: 463 PLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDK 522
             +    +K HM +MFA+HA++M+NTL + ++VFTRTG MA+LLSHYRPS T FAFTN +
Sbjct: 458 SASRTTAYKGHMGQMFAFHASIMANTLSSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQR 517

Query: 523 TIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFP 554
            I QRLALYQGV PIYM+FSDDAE T+A +L LLQ + M+KEG+ V L+QSG QPIWR  
Sbjct: 518 RIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREE 567

[Close]

BLAST of MS023999 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 308.1 bits (788), Expect = 1.4e-83
Identity = 200/494 (40.49%), Postives = 272/494 (55.06%), Query Frame = 0


Query: 104 RKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNC 163
           R+TK++CTIGP+T   E +  LA  GMNVARLNM HG    H+ VI  VR  N + +   
Sbjct: 117 RRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLN-EEKGFA 176

Query: 164 IAIMLDTKASSFSYFHMFCTFINLATNGPEVRSGDL--PQPILLETGQEFTFSIRR-GVS 223
           +AIM+DT+                   G E+  GDL        E G+ +TF++R    S
Sbjct: 177 VAIMMDTE-------------------GSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSS 236

Query: 224 TPD-SVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLN- 283
            P+ ++SV+YD F  DV VGD LLVDGGM+   V  K    VKC   D G L  R +L  
Sbjct: 237 RPERTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTF 296

Query: 284 ------VRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQV-------------- 343
                 VR ++A LP+I+ KDW DI FG+   VDF AVSFVK A+V              
Sbjct: 297 WRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRG 356

Query: 344 ---------------------------AMVARGDLGAELPIEEVPLLQEEIINMCRGMGK 403
                                      AMVARGDLGA++P+E+VP  Q+ I+ +CR + K
Sbjct: 357 GEIGVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNK 416

Query: 404 AVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHTVA 463
            VIVA+ +LESMI +PTPTRAEV+D++ AVR+ +DA+MLSGE+A G+FP KA+ V+ TV+
Sbjct: 417 PVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVS 476

Query: 464 LRTE----ATIAGGQMPLN-LGQTFKNHMSEMFAYHATMMSNTLGT-SIVVFTRTGFMAI 523
           LR E           +PL  +G +F + +SE     A  M+N LG  ++ V+T +G MA 
Sbjct: 477 LRIERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMAS 536

Query: 524 LLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQGMVKE 540
           L+S  RP    FAFT   ++++RL L  G+ P  + FSDD E       SLL+ +GM+K 
Sbjct: 537 LVSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKS 590

[Close]

BLAST of MS023999 vs. TAIR 10
Match: AT3G25960.1 (Pyruvate kinase family protein )

HSP 1 Score: 232.3 bits (591), Expect = 9.7e-61
Identity = 168/496 (33.87%), Postives = 249/496 (50.20%), Query Frame = 0


Query: 95  GMWSKPTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVRE 154
           G  +   +R KTKIVCT+GP++ + EMI KL +AGMNVAR N SHG H+ HQ+ +D +R 
Sbjct: 7   GQATNGALRSKTKIVCTLGPASRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLRT 66

Query: 155 YNAQSQDNCIAIMLDTKASSFSYFHMFCTFINLATNGPEVRSGDLPQ--PILLETGQEFT 214
               +   C A+MLDTK+                   P +R+G L +  PI L+ GQE T
Sbjct: 67  AMDNTGILC-AVMLDTKS-------------------PVIRTGFLKEGKPIQLKQGQEIT 126

Query: 215 FSIRRGV-STPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKS--KTEDSVKCEVVDGG 274
            SI   +    +++S++Y     D++ GD++L   G +SL V S  K    V+C   +  
Sbjct: 127 ISIDYKIQGDSNTISMSYKKLAEDLKPGDVILCSDGTISLNVLSCDKYLGLVRCRCENSA 186

Query: 275 ELKSRRHLNVRGKSATLPSITEKDWDDI-KFGVDNKVDFYAVSFVK-------------- 334
            L  R+++N+ G    LP++TEKD +DI ++GV NK+D  A+SFV+              
Sbjct: 187 LLGERKNVNLPGIVVDLPTLTEKDKEDIMQWGVPNKIDIIALSFVRKGSDLIQVRKLLGE 246

Query: 335 -------------------------DAQVAMVARGDLGAELPIEEVPLLQEEIINMCRGM 394
                                    ++   MVARGDLG E+PIE++ L Q+ +IN     
Sbjct: 247 HSKSIMLMSKVENQEGVMNFDKILENSDAFMVARGDLGMEIPIEKMFLAQKTMINKANAH 306

Query: 395 GKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKVMHT 454
           GK V+ AT MLESM V P PTRAE +D+A AV +G D VMLSGETA G  P  AV  M  
Sbjct: 307 GKPVVTATQMLESMTVSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSR 366

Query: 455 VALRTEATIAGG---QMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAIL 514
           +    E  I      +  L +     + +  + A   +   +   ++IVV TR G+ A L
Sbjct: 367 ICKEAEDFIDYDILHKKTLGMVSLPLSPIESLAASAVSTARSVFASAIVVLTRGGYTAEL 426

Query: 515 LSHYRPS-------GTTFAFTNDKT--IQQRLALYQGVCPIY-MQFSDDAEKTFADALSL 533
           ++ YRPS           A  +D    + +R  +Y+G+ P+      D  E+    A+  
Sbjct: 427 VAKYRPSVPILSVIMPEIAECSDSVAHVARRGLIYRGIIPVVGCSARDSTEEMIRLAIGF 482

[Close]

BLAST of MS023999 vs. TAIR 10
Match: AT3G55650.1 (Pyruvate kinase family protein )

HSP 1 Score: 228.8 bits (582), Expect = 1.1e-59
Identity = 172/512 (33.59%), Postives = 250/512 (48.83%), Query Frame = 0


Query: 95  GMWSKPTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVRE 154
           G  +   +R KTKI+CT+GP + + EMI KL +AGMNVAR N SHG H+ HQ+ +D +R 
Sbjct: 7   GQATNGALRSKTKIICTLGPVSRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLR- 66

Query: 155 YNAQSQDN---CIAIMLDTKASSFSYFHMFCTFINLATNGPEVRSGDLPQ--PILLETGQ 214
               + DN     A+MLDTK                   GPE+R+G L +  PI L  GQ
Sbjct: 67  ---TAMDNTGILSAVMLDTK-------------------GPEIRTGFLKEGKPIQLNQGQ 126

Query: 215 EFTFSIRRGVSTPDSV-SVNYDDFVNDVEVGDMLLVDGGMMSLMVKS--KTEDSVKCEVV 274
           E T SI   +    +V S++Y     DV+ GD++L   G +SL V S  K+   V+C   
Sbjct: 127 EITISIDYMIEGDSNVISMSYKKLAEDVKPGDVILCSDGTISLTVLSCDKSFGLVRCRCE 186

Query: 275 DGGELKSRRHLNVRGKSATLPSITEKDWDD-IKFGVDNKVDFYAVSFVK----------- 334
           +   L  R+++N+ G    LP++TEKD +D I++GV NK+D  A+SFV+           
Sbjct: 187 NSAILGERKNVNLPGIVVDLPTLTEKDKEDIIQWGVPNKIDIIALSFVRKGSDLTEVRRL 246

Query: 335 ----------------------------DAQVAMVARGDLGAELPIEEVPLLQEEIINMC 394
                                       ++   MVARGDLG E+PIE++ L Q+ +I M 
Sbjct: 247 LGEHSKNIMLMSKVENQEGVMNCEKILENSDAFMVARGDLGMEIPIEKMFLAQKTMIKMA 306

Query: 395 RGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAVKV 454
             +GK V+ AT MLESM V P PTRAE +D+A AV +G D VMLSGETA G  P  AV  
Sbjct: 307 NALGKPVVTATQMLESMTVSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPEAAVLT 366

Query: 455 MHTVALRTEATIAGG---QMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFM 514
           M  +    E  I      +  L +     + +  + A   +   +   ++IVV T+ G+ 
Sbjct: 367 MSRICKEAEDFIDYDILHKKTLGMVSLPLSPIESLAASVVSTAQSVFASAIVVLTKGGYT 426

Query: 515 AILLSHYRPS-------GTTFAFTNDKTIQ---------QRLALYQGVCPIYMQFS---- 533
           A L++ YRPS           A  ND  +          +R  +Y+ + P+    S    
Sbjct: 427 AELVAKYRPSVPILSVIVPEIAQGNDMEMSCSDSVAHAARRGLIYRRIIPVVATGSSARD 486

The following BLAST results are available for this feature:

BLAST of MS023999 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140989.1	1.5e-294	90.20	plastidial pyruvate kinase 2 [Momordica charantia]	[more]
XP_038874991.1	4.0e-282	85.98	plastidial pyruvate kinase 2 [Benincasa hispida]	[more]
XP_008439317.1	8.8e-282	85.98	PREDICTED: plastidial pyruvate kinase 2 [Cucumis melo]	[more]
TYK23021.1	2.6e-281	85.81	plastidial pyruvate kinase 2 [Cucumis melo var. makuwa]	[more]
KAG7014734.1	4.4e-281	83.20	Plastidial pyruvate kinase 2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MS023999 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLW9	8.1e-238	73.08	Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1	[more]
Q40546	3.1e-181	67.89	Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q93Z53	4.1e-181	68.37	Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P...	[more]
P55964	2.6e-151	66.59	Pyruvate kinase isozyme G, chloroplastic (Fragment) OS=Ricinus communis OX=3988 ...	[more]
Q9LIK0	2.0e-82	40.49	Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P...	[more]

BLAST of MS023999 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CHB6	7.5e-295	90.20	Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111011509 PE=3 SV=1	[more]
A0A1S3AY45	4.3e-282	85.98	Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103484139 PE=3 SV=1	[more]
A0A5D3DH87	1.2e-281	85.81	Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G0013...	[more]
A0A6J1E5B9	6.2e-281	85.30	Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430719 PE=3 SV=1	[more]
A0A6J1J4F4	1.8e-280	85.30	Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482565 PE=3 SV=1	[more]

BLAST of MS023999 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52920.1	5.8e-239	73.08	plastidic pyruvate kinase beta subunit 1	[more]
AT1G32440.1	2.9e-182	68.37	plastidial pyruvate kinase 3	[more]
AT3G22960.1	1.4e-83	40.49	Pyruvate kinase family protein	[more]
AT3G25960.1	9.7e-61	33.87	Pyruvate kinase family protein	[more]
AT3G55650.1	1.1e-59	33.59	Pyruvate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001697	Pyruvate kinase	PRINTS	PR01050	PYRUVTKNASE	coord: 392..408 score: 55.66 coord: 162..178 score: 30.14 coord: 323..347 score: 53.05 coord: 348..372 score: 62.22 coord: 373..391 score: 65.02
IPR001697	Pyruvate kinase	PANTHER	PTHR11817	PYRUVATE KINASE	coord: 319..553
IPR001697	Pyruvate kinase	PANTHER	PTHR11817	PYRUVATE KINASE	coord: 36..318
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 318..412 e-value: 8.7E-43 score: 148.3
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 104..317 e-value: 7.3E-36 score: 125.7
IPR015795	Pyruvate kinase, C-terminal	PFAM	PF02887	PK_C	coord: 440..533 e-value: 2.6E-14 score: 53.5
IPR015793	Pyruvate kinase, barrel	PFAM	PF00224	PK	coord: 104..173 e-value: 2.1E-22 score: 79.5 coord: 314..419 e-value: 3.3E-42 score: 144.6
IPR036918	Pyruvate kinase, C-terminal domain superfamily	GENE3D	3.40.1380.20		coord: 425..553 e-value: 1.3E-26 score: 94.5
IPR036918	Pyruvate kinase, C-terminal domain superfamily	SUPERFAMILY	52935	PK C-terminal domain-like	coord: 402..553
IPR015806	Pyruvate kinase, insert domain superfamily	GENE3D	2.40.33.10		coord: 192..286 e-value: 4.1E-22 score: 79.8
None	No IPR available	PANTHER	PTHR11817:SF2	PLASTIDIAL PYRUVATE KINASE 2	coord: 36..318 coord: 319..553
IPR011037	Pyruvate kinase-like, insert domain superfamily	SUPERFAMILY	50800	PK beta-barrel domain-like	coord: 192..283
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 96..418

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS023999.1	MS023999.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0030955	potassium ion binding
molecular_function	GO:0004743	pyruvate kinase activity