MS023969 (gene) Bitter gourd (TR) v1

Overview


Name	MS023969
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold44: 341711 .. 343827 (-)
RNA-Seq Expression	MS023969
Synteny	MS023969

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTCCATTTTGCCTTACTTTTCATCTTCTCCATTAATTTTTTGAGCTCGTGGATTGTATCAGCTTCACCCAGCTTCAATGTTCTTGATTATGGAGCCATTGGAAATGGGCAAGCTGATGATTCACAAGTAAAATAATTTAATCTACATTTTGAACTACTTCTAATTCTTTCTTTATCTTCTTCTTTATATAATTGTTTTTCTTTCTTTTTTATTTCTTTATAAGGCTTTTTTGAGAGCTTGGAATGATGTCTGTGGAGCAACAGAAGGAATTCCAACATTACATGCACCACATGGAAAAATTTATATGTTGAAGCCTCTCAAATTTCAGGGTCCTTGCAAGTCCAAACAAGTTACTTTCCATGTAAATTTCTTTCTCATCATTTTTTGTCACATTTTTTTTATTATATGTTTGGACATATCAAGCTTAGAAAATGTTATGGTTTTGTGATTTTGTAGCTTAAAGGGACTCTAGTAGCTCCAAAAAAAAGTTCATGGTCAAACGACGACAGAGAGAGATGGGTTCAATTTTCAGACATAGATGGTCTCATTGTCAATGGAACAGGACAATTTGATGGCCAAGGCTCTTCTTGGTGGGAAGATTGTGAACACTGTCAACGACCAACCGTAAGTTTTATTCGATCATCTTTCCATCGTCTTATCTTACTATACATATTATATATAAAAGTGTAGTGTGCTTAAAGAGTTTTTATGAGTGAAATGAAAAGCTAAGGTAAAAAAGTACCAGATAACCCCTGTACGTCTGATCAAATAAATTTTTACTACATATTATATATAAAATTGTGGTCTACTTAGAGGGAATTTAGGAGTAAAATAAAAATATATGAAAAAAGGTACCAAATAACACTTGATAGATTTTATCCTAACTAGAATTTGATTTTAATGGTTGAATTTATAATGTATGGTTTTGCAGGCACTATTTTTTCACAATTGCAATGGGCTCCAACTAAGGGAAATGAAGCATATTAATAGTGCCAAAAATCATATCTCCATAAATGCTTGTAATGATGTCATTATCTCAGACCTTCACATTATTGCTCCTGAAGATAGCCCTAACACAGATGGAATTGATGTTTCTAGATCAAACAATGTTCTTATTCAAAATTCTTTCATGGGAACAGGTAATTAATTAATTAATTAATTAGCATTTTTCATGATCATTTTTTATTCTTTTTTTTATTCATTACATACATTCAAGGTTAAAATAACCAATTTTATTAATTTCATACTAATTTCTATATGTAGGTGACGATTGTATTGCCCTTAACAATGGATCTTCCAATATTGATATTGTAGGTGTAACTTGTGGCCCAGGCCATGGCATAAGGTAAAAATGATGTTTTCTAGTAACAGTAAGTAATTACTATTACCAAAAAAAAATTACATTTGACCATTTTTATCTCTCATTCCTTATATATATTTTTTATTTTTGTATTTATTTGAAAACAGTATTGGGAGCTTGGGAGGAAATAGAGAATACAACGTAGTTGAAAACATTCATGTAAAAAATTGCCTCTTAAAGGGCACTCAAAATGGAATGAGAATCAAGACTTGGCAGGTGATAATTGTTTCTAGCTCAATTTATTATTATAAAATAATGTTTGTAAATAAATTTCTAGCACATAACTTTTTGTTTTTAATAACGTTTTGAGTTTGTAGGGTGGATATGGATATGCAAGGAACATCACATTCGAACAAATAACACTAATGGGTGCGAAAAACCCGATCATTATTGATCAATATTATACCGGTCTTGCAAAGGTGCAAAAAAACAAGGTAAAATATTTGGTGTAGTTCTCATTAATTTATCTACTGTGATCACTGCTTTTATATAATTTATAAATTTCTTTTCAATATTTTTTCAGTTATTTGACATAAAAGTGAGCAACGTGACTTATCGTGAAGTTAATGGAACGTCAGCAAATGAGAATGCAATTATTTTGAATTGCTCTCGAAGTCGCTGCAGCAATATTATTTTAGAAAATGTGAGCATAAAGCCAACCGATCCAAAAATGGAAGCCAAAGCTTTTTGTCAAAATGTTGATGGAAGATCTACTTATGCAGTTCCTACTGTTCCTTGTTTATCAAATTAG

mRNA sequence

ATGAAGCTCCATTTTGCCTTACTTTTCATCTTCTCCATTAATTTTTTGAGCTCGTGGATTGTATCAGCTTCACCCAGCTTCAATGTTCTTGATTATGGAGCCATTGGAAATGGGCAAGCTGATGATTCACAACTTAAAGGGACTCTAGTAGCTCCAAAAAAAAGTTCATGGTCAAACGACGACAGAGAGAGATGGGTTCAATTTTCAGACATAGATGGTCTCATTGTCAATGGAACAGGACAATTTGATGGCCAAGGCTCTTCTTGGTGGGAAGATTGTGAACACTGTCAACGACCAACCGCACTATTTTTTCACAATTGCAATGGGCTCCAACTAAGGGAAATGAAGCATATTAATAGTGCCAAAAATCATATCTCCATAAATGCTTGTAATGATGTCATTATCTCAGACCTTCACATTATTGCTCCTGAAGATAGCCCTAACACAGATGGAATTGATGTTTCTAGATCAAACAATGTTCTTATTCAAAATTCTTTCATGGGAACAGGTGACGATTGTATTGCCCTTAACAATGGATCTTCCAATATTGATATTGTAGGTGTAACTTGTGGCCCAGGCCATGGCATAAGTATTGGGAGCTTGGGAGGAAATAGAGAATACAACGTAGTTGAAAACATTCATGTAAAAAATTGCCTCTTAAAGGGCACTCAAAATGGAATGAGAATCAAGACTTGGCAGGGTGGATATGGATATGCAAGGAACATCACATTCGAACAAATAACACTAATGGGTGCGAAAAACCCGATCATTATTGATCAATATTATACCGGTCTTGCAAAGGTGCAAAAAAACAAGTTATTTGACATAAAAGTGAGCAACGTGACTTATCGTGAAGTTAATGGAACGTCAGCAAATGAGAATGCAATTATTTTGAATTGCTCTCGAAGTCGCTGCAGCAATATTATTTTAGAAAATGTGAGCATAAAGCCAACCGATCCAAAAATGGAAGCCAAAGCTTTTTGTCAAAATGTTGATGGAAGATCTACTTATGCAGTTCCTACTGTTCCTTGTTTATCAAATTAG

Coding sequence (CDS)

ATGAAGCTCCATTTTGCCTTACTTTTCATCTTCTCCATTAATTTTTTGAGCTCGTGGATTGTATCAGCTTCACCCAGCTTCAATGTTCTTGATTATGGAGCCATTGGAAATGGGCAAGCTGATGATTCACAACTTAAAGGGACTCTAGTAGCTCCAAAAAAAAGTTCATGGTCAAACGACGACAGAGAGAGATGGGTTCAATTTTCAGACATAGATGGTCTCATTGTCAATGGAACAGGACAATTTGATGGCCAAGGCTCTTCTTGGTGGGAAGATTGTGAACACTGTCAACGACCAACCGCACTATTTTTTCACAATTGCAATGGGCTCCAACTAAGGGAAATGAAGCATATTAATAGTGCCAAAAATCATATCTCCATAAATGCTTGTAATGATGTCATTATCTCAGACCTTCACATTATTGCTCCTGAAGATAGCCCTAACACAGATGGAATTGATGTTTCTAGATCAAACAATGTTCTTATTCAAAATTCTTTCATGGGAACAGGTGACGATTGTATTGCCCTTAACAATGGATCTTCCAATATTGATATTGTAGGTGTAACTTGTGGCCCAGGCCATGGCATAAGTATTGGGAGCTTGGGAGGAAATAGAGAATACAACGTAGTTGAAAACATTCATGTAAAAAATTGCCTCTTAAAGGGCACTCAAAATGGAATGAGAATCAAGACTTGGCAGGGTGGATATGGATATGCAAGGAACATCACATTCGAACAAATAACACTAATGGGTGCGAAAAACCCGATCATTATTGATCAATATTATACCGGTCTTGCAAAGGTGCAAAAAAACAAGTTATTTGACATAAAAGTGAGCAACGTGACTTATCGTGAAGTTAATGGAACGTCAGCAAATGAGAATGCAATTATTTTGAATTGCTCTCGAAGTCGCTGCAGCAATATTATTTTAGAAAATGTGAGCATAAAGCCAACCGATCCAAAAATGGAAGCCAAAGCTTTTTGTCAAAATGTTGATGGAAGATCTACTTATGCAGTTCCTACTGTTCCTTGTTTATCAAATTAG

Protein sequence

MKLHFALLFIFSINFLSSWIVSASPSFNVLDYGAIGNGQADDSQLKGTLVAPKKSSWSNDDRERWVQFSDIDGLIVNGTGQFDGQGSSWWEDCEHCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTGLAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRSRCSNIILENVSIKPTDPKMEAKAFCQNVDGRSTYAVPTVPCLSN

Homology

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BLAST of MS023969 vs. NCBI nr
Match: XP_022141107.1 (probable polygalacturonase At3g15720 [Momordica charantia])

HSP 1 Score: 642.9 bits (1657), Expect = 1.6e-180
Identity = 327/393 (83.21%), Postives = 331/393 (84.22%), Query Frame = 0


Query: 1   MKLHFALLFIFSINFLSSWIVSASPSFNVLDYGAIGNGQADDSQ---------------- 60
           MKLHF LLFIFSIN LSSWIVSASPSFNVLDYGAIGNGQADDSQ                
Sbjct: 1   MKLHFVLLFIFSINILSSWIVSASPSFNVLDYGAIGNGQADDSQAFLRAWNDVCGATEGI 60

Query: 61  ------------------------------LKGTLVAPKKSSWSNDDRERWVQFSDIDGL 120
                                         LKGTLVAPKK SW   DRERW+QFSDIDGL
Sbjct: 61  PTLHAPHGKIYMLKPLKFQGPCKSKQVTFHLKGTLVAPKKGSWPKYDRERWIQFSDIDGL 120

Query: 121 IVNGTGQFDGQGSSWWEDCEHCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDVI 180
           IVNGTGQFDGQGSSWWED EHCQRPTALF HNCNGLQLREMKHINSAKNHISINACNDVI
Sbjct: 121 IVNGTGQFDGQGSSWWEDWEHCQRPTALFLHNCNGLQLREMKHINSAKNHISINACNDVI 180

Query: 181 ISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGH 240
           ISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGH
Sbjct: 181 ISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGH 240

Query: 241 GISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKN 300
           GISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKN
Sbjct: 241 GISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKN 300

Query: 301 PIIIDQYYTGLAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRSRCSNIILENVS 348
           PIIIDQYYT    +   +LFDIKVSNVTYREVNGTSANENAIILNCSR+RCSNIILENVS
Sbjct: 301 PIIIDQYYTDFFSI-FFQLFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENVS 360

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BLAST of MS023969 vs. NCBI nr
Match: XP_022141108.1 (probable polygalacturonase At3g15720 [Momordica charantia])

HSP 1 Score: 578.6 bits (1490), Expect = 3.7e-161
Identity = 288/393 (73.28%), Postives = 316/393 (80.41%), Query Frame = 0


Query: 1   MKLHFALLFIFSINFLSSWIVSASPSFNVLDYGAIGNGQADDSQ---------------- 60
           MKLH AL+FIFSI+ LSSW+VS SP+FNVLDYGAIGNGQ DDSQ                
Sbjct: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQAFLRAWXDACGATEGI 60

Query: 61  ------------------------------LKGTLVAPKKSSWSNDDRERWVQFSDIDGL 120
                                         L GT+VAP K SW +DDR+RW+QFSDIDGL
Sbjct: 61  PALHAPVGKIYMLNPLKFQGPCKSKQVIFHLGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120

Query: 121 IVNGTGQFDGQGSSWWEDCE-HCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDV 180
           +VNG+GQFDGQGSSWW+DC+ HC RPTALF HNCNGLQLREMKHINS +NHISINACND 
Sbjct: 121 MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA 180

Query: 181 IISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPG 240
           IISDLHI+APEDSPNTDGID+S SNNVLIQNSFMGTGDDCIALNNGS+NI+IVGVTCGPG
Sbjct: 181 IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG 240

Query: 241 HGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAK 300
           HGISIGSLG N +YNVVE+IHVKNCLLKGTQNG+RIKTWQ GYGYAR ITFEQITL+  K
Sbjct: 241 HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK 300

Query: 301 NPIIIDQYYTGLAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRSRCSNIILENV 347
           NPIIIDQYY+ LAK QKN+L  IKVS+VTYREV+GTSANENAIILNCSRSRCSNII+ENV
Sbjct: 301 NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV 360

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BLAST of MS023969 vs. NCBI nr
Match: XP_038888688.1 (probable polygalacturonase At3g15720 [Benincasa hispida])

HSP 1 Score: 500.0 bits (1286), Expect = 1.7e-137
Identity = 246/394 (62.44%), Postives = 296/394 (75.13%), Query Frame = 0


Query: 1   MKLHFALLFIFSINFLSSWIVSASPS-FNVLDYGAIGNGQADDS---------------- 60
           MKL F  + IFSI  LSS  V ASP  FNVL YGA GN + DDS                
Sbjct: 1   MKLLFVFVSIFSIANLSSRNVLASPHYFNVLHYGATGNDETDDSQAFLKAWKDVCEATKG 60

Query: 61  ------------------------------QLKGTLVAPKKSSWSNDDRERWVQFSDIDG 120
                                         QL+GTLVAPKK +W  D +++W++F DIDG
Sbjct: 61  IPTLHVPLGKSYLLNPLTFQGPCKSKQVNIQLRGTLVAPKKGAWPTDKKDKWIEFHDIDG 120

Query: 121 LIVNGTGQFDGQGSSWWEDCE-HCQRPTALFFHNCNGLQLREMKHINSAKNHISINACND 180
           L +NG GQ DGQGSSWWEDCE HC+RPTALFFHNCNG  LR+MKH+NS +NHISINACN+
Sbjct: 121 LTINGGGQIDGQGSSWWEDCETHCERPTALFFHNCNGFLLRDMKHVNSGRNHISINACNN 180

Query: 181 VIISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGP 240
           V++S++HIIAPE SPNTDGID++RS NV IQ SFM TGDDC+A+NNGSSNI+IVG+TCGP
Sbjct: 181 VMVSNIHIIAPEASPNTDGIDIARSTNVFIQKSFMATGDDCVAINNGSSNINIVGITCGP 240

Query: 241 GHGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGA 300
           GHGISIGSLG + +YNVVE++HV+NC  KG QNG+RIKTW+GGYGYARNITFE+ITLM  
Sbjct: 241 GHGISIGSLGADGQYNVVEDVHVRNCFFKGAQNGVRIKTWEGGYGYARNITFEKITLMNT 300

Query: 301 KNPIIIDQYYTGLAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRSRCSNIILEN 347
           +NPIIIDQYYT  A  +KNK+ +IKVS+VTYREV+GTSANENAI LNCSR+RC+NI+++N
Sbjct: 301 RNPIIIDQYYTTSASSRKNKVINIKVSDVTYREVHGTSANENAITLNCSRARCTNILMDN 360

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BLAST of MS023969 vs. NCBI nr
Match: XP_038888676.1 (probable polygalacturonase At3g15720 [Benincasa hispida])

HSP 1 Score: 495.4 bits (1274), Expect = 4.1e-136
Identity = 249/394 (63.20%), Postives = 296/394 (75.13%), Query Frame = 0


Query: 1   MKLHFALLFIFSINFLSSWIVSASPSFNVLDYGAIGNGQADDSQ---------------- 60
           MKL FAL+FIF+I+ L+  IVSAS +FNVLDYGAIGNG+ADDS+                
Sbjct: 1   MKLLFALIFIFTIDILTLSIVSASSNFNVLDYGAIGNGKADDSEAFLKAWNDVCGATEDS 60

Query: 61  ------------------------------LKGTLVAPKKSSWSNDDRERWVQFSDIDGL 120
                                         L+GTL+AP K  W +D  ++WVQF DI+GL
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSNQVNFDLRGTLMAPSKDEWPSDGADKWVQFYDIEGL 120

Query: 121 IVNGTGQFDGQGSSWWEDCE--HCQRPTALFFHNCNGLQLREMKHINSAKNHISINACND 180
            + G G+FDGQGS WW+DC+  HC+RPTALFFHNCNGLQ++EMKHINS +NHISIN CND
Sbjct: 121 NLKGAGKFDGQGSLWWKDCDRKHCKRPTALFFHNCNGLQVKEMKHINSGRNHISINDCND 180

Query: 181 VIISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGP 240
           VI S++ I+APE+SPNTDGID+S+S NVLIQ+SF+ TGDDCIA+N GSS+I+I+G+TCGP
Sbjct: 181 VIFSNIQILAPEESPNTDGIDISKSKNVLIQDSFIATGDDCIAINEGSSDINIIGITCGP 240

Query: 241 GHGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGA 300
           GHGISIGSLG +  YNVVENIHV NCLL+GTQNG+RIKTWQGGYGYA+NITFE+ITL  A
Sbjct: 241 GHGISIGSLGKDGAYNVVENIHVSNCLLQGTQNGVRIKTWQGGYGYAKNITFEKITLKNA 300

Query: 301 KNPIIIDQYYTGLAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRSRCSNIILEN 347
           KNPIIIDQYYT  A  +K K  DIKVS+VTYREVNGTSANE+AI LNCSR+RC+NIIL+N
Sbjct: 301 KNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEDAITLNCSRARCTNIILDN 360

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BLAST of MS023969 vs. NCBI nr
Match: KGN53196.1 (hypothetical protein Csa_014667 [Cucumis sativus])

HSP 1 Score: 489.2 bits (1258), Expect = 3.0e-134
Identity = 244/390 (62.56%), Postives = 288/390 (73.85%), Query Frame = 0


Query: 1   MKLHFALLFIFSINFLSSWIVSASPSFNVLDYGAIGNGQADDS----------------- 60
           MKL  A +F++SI  LSSW V  S  FNVLDYGAIGNG+ DDS                 
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  -------------------------QLKGTLVAPKKSSWSNDDRERWVQFSDIDGLIVNG 120
                                    QL GTL+AP K  W +D + +W+QF DI+GL + G
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQLGGTLMAPTKDEWISDGKGQWIQFFDIEGLNLQG 120

Query: 121 TGQFDGQGSSWWEDCE--HCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDVIIS 180
            G+FDGQGS WW+DC+  HC+RPTALFFHNCNGLQ++ MKHINSAKNHISIN C+DVI S
Sbjct: 121 GGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHDVIFS 180

Query: 181 DLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGI 240
            LHI APEDSPNTDGID+S S N+ I++SFM TGDDCIA+NNGSSNI+I G+TCGPGHGI
Sbjct: 181 HLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGPGHGI 240

Query: 241 SIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPI 300
           SIGSLG + EYNVVEN+HV NCLL+ TQNG+RIKTW+GGYGYA+NITFE+IT+  AKNPI
Sbjct: 241 SIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNAKNPI 300

Query: 301 IIDQYYTGLAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRSRCSNIILENVSIK 347
           IIDQYY+  A  +K K  DIKVS+VTYREVNGTSANE+AI  NCS++RCSNIIL+NV+IK
Sbjct: 301 IIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDNVNIK 360

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BLAST of MS023969 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.8e-79
Identity = 170/385 (44.16%), Postives = 223/385 (57.92%), Query Frame = 0


Query: 11  FSINFLSSWIVSASPSFNVLDYGAIGNGQADDS--------------------------- 70
           FS+ FL   I ++S + +V  +GA+G+G  DDS                           
Sbjct: 10  FSLFFLQ--IFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFVVPAGMTF 69

Query: 71  ------------------QLKGTLVAPKKSSWSNDDRERWVQFSDIDGLIVNGTGQFDGQ 130
                             Q+ G LVAP K +W   D+++W+ F+DI+GL++ G G+ +GQ
Sbjct: 70  MLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKG-DKDQWILFTDIEGLVIEGDGEINGQ 129

Query: 131 GSSWWEDCEHCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPED 190
           GSSWWE      RPTAL F +CN L+L  + H++S   HI I+ CN V IS L I APE 
Sbjct: 130 GSSWWE--HKGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPES 189

Query: 191 SPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNR 250
           SPNTDGIDV  S+NV+IQ+  + TGDDCIA+N+G+SNI I G+ CGPGHGISIGSLG + 
Sbjct: 190 SPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDG 249

Query: 251 EYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTG- 310
           E   VEN+ V+NC  +GT NG RIKTWQGG GYAR ITF  ITL   +NPIIIDQ+Y G 
Sbjct: 250 ETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGG 309

Query: 311 -LAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCS-RSRCSNIILENVSIK--PTDP 346
                +  K   ++VS V +    GTS +E  +   CS R  C+ I L ++ I+   +  
Sbjct: 310 DSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGS 369

[Close]

BLAST of MS023969 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.3e-65
Identity = 128/313 (40.89%), Postives = 186/313 (59.42%), Query Frame = 0


Query: 44  QLKGTLVAPKKSS-WSNDDRERWVQFSDIDGLIVNGTGQFDGQGSSWWE-DCEH-----C 103
           Q+ GT++AP     W   +R +W+ F  +  L V G G  +G G  WWE  C+H     C
Sbjct: 114 QISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNPC 173

Query: 104 Q-RPTALFFHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVS 163
           +  PTAL FH C  +++  +  I+S + HI++ +C  V IS L +IAP  SPNTDGI +S
Sbjct: 174 RGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHIS 233

Query: 164 RSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHV 223
            S  ++I N+ + TGDDCI++   S+ I I  + CGPGHGISIGSLG ++ +  V +I V
Sbjct: 234 VSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITV 293

Query: 224 KNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTGLAKVQKNKLFD 283
              ++  T NG+RIKTWQGG G    I F  I +    NPIIIDQYY    K   N+   
Sbjct: 294 DTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSA 353

Query: 284 IKVSNVTYREVNGTSANENAIILNCS-RSRCSNIILENVSIKPTDPKMEAKAFCQNVDGR 343
           I + N+++  V GTSA++ AI ++CS  S C NI+L+++ ++P++     ++FC    G 
Sbjct: 354 ISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGS 413

Query: 344 STYAVPTVPCLSN 348
           S+  V   PCLS+
Sbjct: 414 SSGQVYPPPCLSD 426

[Close]

BLAST of MS023969 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-61
Identity = 131/301 (43.52%), Postives = 184/301 (61.13%), Query Frame = 0


Query: 44  QLKGTLVAPKKSSWSNDDRERWVQFSDIDGLIVNG--TGQFDGQGSSWWEDCEHCQR--- 103
           Q+ GTL A +K S    D  +W+ F  ++ L V+G  TG  DG G +WW++   C+R   
Sbjct: 129 QIFGTLSASQKRS-DYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQN--SCKRNKA 188

Query: 104 ------PTALFFHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGI 163
                 PTAL F+N   L ++ +K  N+ +  ISI  C++V +S++ + AP DSPNTDGI
Sbjct: 189 KPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTDGI 248

Query: 164 DVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVEN 223
            ++ + N+ +  S +GTGDDCI++ +GS N+ I  +TCGPGHGISIGSLG +     V  
Sbjct: 249 HITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFVSG 308

Query: 224 IHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTGLAKVQKNK 283
           + V    L GT NG+RIKT+QGG G A NI F+ I +   KNPIIIDQ Y   +K    K
Sbjct: 309 VTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEK 368

Query: 284 LFDIKVSNVTYREVNGTSANENAIILNCSRS-RCSNIILENVSIKPTDPKMEAKAFCQNV 333
              ++V NV YR+++GTSA+ENAI  NCS++  C  I+L+ V+IK        KA C N 
Sbjct: 369 -SAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIK------GGKATCTNA 419

[Close]

BLAST of MS023969 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.9e-60
Identity = 126/311 (40.51%), Postives = 179/311 (57.56%), Query Frame = 0


Query: 44  QLKGTLVAPK-KSSWSNDDRERWVQFSDIDGLIVNGTGQFDGQGSSWWEDCEHCQR---- 103
           +++GT+ A   +S W   +R+RW++F DI  L + G G  +G G +WW+    C+R    
Sbjct: 143 KIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWD--SSCKRKKSL 202

Query: 104 -----PTALFFHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGID 163
                PTAL F +C  L + ++   +S K H+S + C DVI S+L + APE SPNTDGI 
Sbjct: 203 PCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSPNTDGIH 262

Query: 164 VSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENI 223
           ++ +  + + NS +GTGDDCI++ +GS  +    +TCGPGHGISIGSLG       V  +
Sbjct: 263 ITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEAHVSGV 322

Query: 224 HVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTGLAKVQKNKL 283
            V    L  T NG+RIKTWQGG G A+NI F+ I +    NPIIIDQYY         + 
Sbjct: 323 LVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDPCPEQE 382

Query: 284 FDIKVSNVTYREVNGTSANENAIILNCSRSR-CSNIILENVSIKPTDPKMEAKAFCQNV- 338
             +KVSNV Y  + GTSA+E A+  +CS+S  C   I+ N+++     K E    C N+ 
Sbjct: 383 SAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNGGK-ETTMSCSNIV 442

[Close]

BLAST of MS023969 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 8.6e-60
Identity = 139/380 (36.58%), Postives = 193/380 (50.79%), Query Frame = 0


Query: 23  ASPSFNVLDYGAIGNGQADDSQ-------------------------------------- 82
           A+  FNV  YGA+G+G+ D ++                                      
Sbjct: 55  AATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQP 114

Query: 83  -----LKGTLVA-PKKSSWSNDDRERWVQFSDIDGLIVNGTGQFDGQGSSWWED------ 142
                + GT+VA P  + W N   + W+QF+ +    + GTG  DGQG  WW        
Sbjct: 115 HLSFKVDGTIVAQPDPARWKNS--KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKVVN 174

Query: 143 ----CEHCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPN 202
               C    RPTA+       + ++E+  +NS + H+    C  V I  L I AP DSPN
Sbjct: 175 GRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSPN 234

Query: 203 TDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYN 262
           TDGID+  S    I+   +GTGDDCIA+  GSSNI I  + CGPGHGISIGSLG +    
Sbjct: 235 TDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSRA 294

Query: 263 VVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTGLAKV 322
            V ++HV       TQNG+RIKTWQGG G A  IT+E + ++ ++NPI+I+Q+Y   A  
Sbjct: 295 EVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSASA 354

Query: 323 QKNKLFDIKVSNVTYREVNGTSANENAIILNCSRS-RCSNIILENVSIKPTDPKMEAKAF 348
            +N+   +++  VTY+ ++GTSA   AI L CS S  C+ I L NVS+K T  K    A 
Sbjct: 355 CQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLKLTSGK---PAS 414

[Close]

BLAST of MS023969 vs. ExPASy TrEMBL
Match: A0A6J1CIX8 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011581 PE=3 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 7.8e-181
Identity = 327/393 (83.21%), Postives = 331/393 (84.22%), Query Frame = 0


Query: 1   MKLHFALLFIFSINFLSSWIVSASPSFNVLDYGAIGNGQADDSQ---------------- 60
           MKLHF LLFIFSIN LSSWIVSASPSFNVLDYGAIGNGQADDSQ                
Sbjct: 1   MKLHFVLLFIFSINILSSWIVSASPSFNVLDYGAIGNGQADDSQAFLRAWNDVCGATEGI 60

Query: 61  ------------------------------LKGTLVAPKKSSWSNDDRERWVQFSDIDGL 120
                                         LKGTLVAPKK SW   DRERW+QFSDIDGL
Sbjct: 61  PTLHAPHGKIYMLKPLKFQGPCKSKQVTFHLKGTLVAPKKGSWPKYDRERWIQFSDIDGL 120

Query: 121 IVNGTGQFDGQGSSWWEDCEHCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDVI 180
           IVNGTGQFDGQGSSWWED EHCQRPTALF HNCNGLQLREMKHINSAKNHISINACNDVI
Sbjct: 121 IVNGTGQFDGQGSSWWEDWEHCQRPTALFLHNCNGLQLREMKHINSAKNHISINACNDVI 180

Query: 181 ISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGH 240
           ISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGH
Sbjct: 181 ISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGH 240

Query: 241 GISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKN 300
           GISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKN
Sbjct: 241 GISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKN 300

Query: 301 PIIIDQYYTGLAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRSRCSNIILENVS 348
           PIIIDQYYT    +   +LFDIKVSNVTYREVNGTSANENAIILNCSR+RCSNIILENVS
Sbjct: 301 PIIIDQYYTDFFSI-FFQLFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENVS 360

[Close]

BLAST of MS023969 vs. ExPASy TrEMBL
Match: A0A6J1CJJ6 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011582 PE=3 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.8e-161
Identity = 288/393 (73.28%), Postives = 316/393 (80.41%), Query Frame = 0


Query: 1   MKLHFALLFIFSINFLSSWIVSASPSFNVLDYGAIGNGQADDSQ---------------- 60
           MKLH AL+FIFSI+ LSSW+VS SP+FNVLDYGAIGNGQ DDSQ                
Sbjct: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQAFLRAWXDACGATEGI 60

Query: 61  ------------------------------LKGTLVAPKKSSWSNDDRERWVQFSDIDGL 120
                                         L GT+VAP K SW +DDR+RW+QFSDIDGL
Sbjct: 61  PALHAPVGKIYMLNPLKFQGPCKSKQVIFHLGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120

Query: 121 IVNGTGQFDGQGSSWWEDCE-HCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDV 180
           +VNG+GQFDGQGSSWW+DC+ HC RPTALF HNCNGLQLREMKHINS +NHISINACND 
Sbjct: 121 MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA 180

Query: 181 IISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPG 240
           IISDLHI+APEDSPNTDGID+S SNNVLIQNSFMGTGDDCIALNNGS+NI+IVGVTCGPG
Sbjct: 181 IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG 240

Query: 241 HGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAK 300
           HGISIGSLG N +YNVVE+IHVKNCLLKGTQNG+RIKTWQ GYGYAR ITFEQITL+  K
Sbjct: 241 HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK 300

Query: 301 NPIIIDQYYTGLAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRSRCSNIILENV 347
           NPIIIDQYY+ LAK QKN+L  IKVS+VTYREV+GTSANENAIILNCSRSRCSNII+ENV
Sbjct: 301 NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV 360

[Close]

BLAST of MS023969 vs. ExPASy TrEMBL
Match: A0A0A0KZ95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.4e-134
Identity = 244/390 (62.56%), Postives = 288/390 (73.85%), Query Frame = 0


Query: 1   MKLHFALLFIFSINFLSSWIVSASPSFNVLDYGAIGNGQADDS----------------- 60
           MKL  A +F++SI  LSSW V  S  FNVLDYGAIGNG+ DDS                 
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  -------------------------QLKGTLVAPKKSSWSNDDRERWVQFSDIDGLIVNG 120
                                    QL GTL+AP K  W +D + +W+QF DI+GL + G
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQLGGTLMAPTKDEWISDGKGQWIQFFDIEGLNLQG 120

Query: 121 TGQFDGQGSSWWEDCE--HCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDVIIS 180
            G+FDGQGS WW+DC+  HC+RPTALFFHNCNGLQ++ MKHINSAKNHISIN C+DVI S
Sbjct: 121 GGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHDVIFS 180

Query: 181 DLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGI 240
            LHI APEDSPNTDGID+S S N+ I++SFM TGDDCIA+NNGSSNI+I G+TCGPGHGI
Sbjct: 181 HLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGPGHGI 240

Query: 241 SIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPI 300
           SIGSLG + EYNVVEN+HV NCLL+ TQNG+RIKTW+GGYGYA+NITFE+IT+  AKNPI
Sbjct: 241 SIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNAKNPI 300

Query: 301 IIDQYYTGLAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRSRCSNIILENVSIK 347
           IIDQYY+  A  +K K  DIKVS+VTYREVNGTSANE+AI  NCS++RCSNIIL+NV+IK
Sbjct: 301 IIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDNVNIK 360

[Close]

BLAST of MS023969 vs. ExPASy TrEMBL
Match: A0A5D3CYS8 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00190 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 6.0e-133
Identity = 244/394 (61.93%), Postives = 285/394 (72.34%), Query Frame = 0


Query: 1   MKLHFALLFIFSINFLSSWIVSASPSFNVLDYGAIGNGQADDSQ---------------- 60
           MKL  A +  +SI  LSS  V +S  FNVLDYGAIGNG+ DDS+                
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  ------------------------------LKGTLVAPKKSSWSNDDRERWVQFSDIDGL 120
                                         L GTL+AP K  W ND + +WVQF D++GL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 IVNGTGQFDGQGSSWWEDCEH--CQRPTALFFHNCNGLQLREMKHINSAKNHISINACND 180
            V G G+FDGQGS WW+ C+H  C+RPTALFFHNCNGLQ+  MKH+NSAKNHISIN CND
Sbjct: 121 NVQGGGRFDGQGSCWWKGCDHKQCKRPTALFFHNCNGLQMNGMKHVNSAKNHISINLCND 180

Query: 181 VIISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGP 240
           VI S LHI APEDSPNTDGID+S+S N+ IQ+SF+GTGDDC+A+NNGSSNI+I  +TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGP 240

Query: 241 GHGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGA 300
           GHGISIGSLG + EYNVVEN+HV NCLL+ TQNG+RIKTW+GGYGYA+NITFE+ITL  +
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNS 300

Query: 301 KNPIIIDQYYTGLAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRSRCSNIILEN 347
           KNPIIIDQYYT  A  +K K  DIKVS+VTYREVNGTSANE AI  NCS++RCSNIIL+N
Sbjct: 301 KNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDN 360

[Close]

BLAST of MS023969 vs. ExPASy TrEMBL
Match: A0A1S3BYT5 (probable polygalacturonase At3g15720 OS=Cucumis melo OX=3656 GN=LOC103494869 PE=3 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 8.4e-127
Identity = 238/392 (60.71%), Postives = 277/392 (70.66%), Query Frame = 0


Query: 1   MKLHFALLFIFSINFLSSWIVSASPSFNVLDYGAIGNGQADDSQ---------------- 60
           MKL  A +  +SI  LSS  V +S  FNVLDYGAIGNG+ DDS+                
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  ------------------------------LKGTLVAPKKSSWSNDDRERWVQFSDIDGL 120
                                         L GTL+AP K  W ND + +WVQF D++GL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 IVNGTGQFDGQGSSWWEDCEHCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDVI 180
            V G G+FDGQGS WW+         ALFFHNCNGLQ+  MKH+NSAKNHISIN CNDVI
Sbjct: 121 NVQGGGRFDGQGSCWWK---------ALFFHNCNGLQMNGMKHVNSAKNHISINLCNDVI 180

Query: 181 ISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGH 240
            S LHI APEDSPNTDGID+S+S N+ IQ+SF+GTGDDC+A+NNGSSNI+I  +TCGPGH
Sbjct: 181 FSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGPGH 240

Query: 241 GISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKN 300
           GISIGSLG + EYNVVEN+HV NCLL+ TQNG+RIKTW+GGYGYA+NITFE+ITL  +KN
Sbjct: 241 GISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNSKN 300

Query: 301 PIIIDQYYTGLAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRSRCSNIILENVS 347
           PIIIDQYYT  A  +K K  DIKVS+VTYREVNGTSANE AI  NCS++RCSNIIL+NV+
Sbjct: 301 PIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDNVN 360

[Close]

BLAST of MS023969 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 297.0 bits (759), Expect = 2.0e-80
Identity = 170/385 (44.16%), Postives = 223/385 (57.92%), Query Frame = 0


Query: 11  FSINFLSSWIVSASPSFNVLDYGAIGNGQADDS--------------------------- 70
           FS+ FL   I ++S + +V  +GA+G+G  DDS                           
Sbjct: 10  FSLFFLQ--IFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFVVPAGMTF 69

Query: 71  ------------------QLKGTLVAPKKSSWSNDDRERWVQFSDIDGLIVNGTGQFDGQ 130
                             Q+ G LVAP K +W   D+++W+ F+DI+GL++ G G+ +GQ
Sbjct: 70  MLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKG-DKDQWILFTDIEGLVIEGDGEINGQ 129

Query: 131 GSSWWEDCEHCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPED 190
           GSSWWE      RPTAL F +CN L+L  + H++S   HI I+ CN V IS L I APE 
Sbjct: 130 GSSWWE--HKGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPES 189

Query: 191 SPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNR 250
           SPNTDGIDV  S+NV+IQ+  + TGDDCIA+N+G+SNI I G+ CGPGHGISIGSLG + 
Sbjct: 190 SPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDG 249

Query: 251 EYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTG- 310
           E   VEN+ V+NC  +GT NG RIKTWQGG GYAR ITF  ITL   +NPIIIDQ+Y G 
Sbjct: 250 ETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGG 309

Query: 311 -LAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCS-RSRCSNIILENVSIK--PTDP 346
                +  K   ++VS V +    GTS +E  +   CS R  C+ I L ++ I+   +  
Sbjct: 310 DSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGS 369

[Close]

BLAST of MS023969 vs. TAIR 10
Match: AT3G15720.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 4.2e-78
Identity = 168/385 (43.64%), Postives = 221/385 (57.40%), Query Frame = 0


Query: 11  FSINFLSSWIVSASPSFNVLDYGAIGNGQADDS--------------------------- 70
           FS+ FL   I ++S + +V  +GA+G+G  DDS                           
Sbjct: 9   FSLFFLQ--IFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFVVPAGMTF 68

Query: 71  ------------------QLKGTLVAPKKSSWSNDDRERWVQFSDIDGLIVNGTGQFDGQ 130
                             Q+ G LVAP K +W   D+++W+ F+DI+GL++ G G+ +GQ
Sbjct: 69  MLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKG-DKDQWILFTDIEGLVIEGDGEINGQ 128

Query: 131 GSSWWEDCEHCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPED 190
           GSSWWE      RPT   F +CN L+L  + H++S   HI I+ CN V IS L I APE 
Sbjct: 129 GSSWWE--HKGSRPT---FRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPES 188

Query: 191 SPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNR 250
           SPNTDGIDV  S+NV+IQ+  + TGDDCIA+N+G+SNI I G+ CGPGHGISIGSLG + 
Sbjct: 189 SPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDG 248

Query: 251 EYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTG- 310
           E   VEN+ V+NC  +GT NG RIKTWQGG GYAR ITF  ITL   +NPIIIDQ+Y G 
Sbjct: 249 ETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGG 308

Query: 311 -LAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCS-RSRCSNIILENVSIK--PTDP 346
                +  K   ++VS V +    GTS +E  +   CS R  C+ I L ++ I+   +  
Sbjct: 309 DSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGS 368

[Close]

BLAST of MS023969 vs. TAIR 10
Match: AT4G35670.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 8.7e-76
Identity = 160/384 (41.67%), Postives = 220/384 (57.29%), Query Frame = 0


Query: 13  INFLSSWIVSASPSFNVLDYGAIGNGQADDS----------------------------- 72
           INFL   IV    ++NVL++ A G+GQ DDS                             
Sbjct: 11  INFLCFGIVYGK-NYNVLNFDAKGDGQTDDSEAFLQAWTAACGGDGDIKTLLIPSGKTFL 70

Query: 73  -----------------QLKGTLVAPK-KSSWSNDDRERWVQFSDIDGLIVNGTGQFDGQ 132
                            QL GT+VAP  K +WS+     W++FS + GLI+ G+G  D +
Sbjct: 71  LQPTVFQGPCKSSSIKVQLDGTIVAPSDKFAWSDPISRMWIKFSTVSGLIIVGSGTIDSR 130

Query: 133 GSSWWE-DCEHCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPE 192
           GSS+WE + +  QRPTAL    C+ L++  +  I+S KNHISI  CN V IS++++ APE
Sbjct: 131 GSSFWELNLKASQRPTALHISKCDNLRINGITSIDSPKNHISIKTCNTVAISNINLFAPE 190

Query: 193 DSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGN 252
            SPNTDGID+S S N+ I +S + TGDDCIA+N+GSSNI+I G+ CGPGHGIS+GSLG  
Sbjct: 191 TSPNTDGIDISDSTNINIFDSTIQTGDDCIAINSGSSNINITGINCGPGHGISVGSLGAG 250

Query: 253 REYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTG 312
                V ++ V +C    T NG RIKTW GG GYARNI+F  ITL+  KNPIIIDQ+Y  
Sbjct: 251 GAEAKVSDVQVTHCTFNQTTNGARIKTWLGGQGYARNISFTDITLVNTKNPIIIDQHYID 310

Query: 313 LAKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSR-SRCSNIILENVSIKPTDPKME 348
             ++ +     + +SNV + +  GTS+N+NAI L CS  + C +++++ + I   +   +
Sbjct: 311 KGRLTEESA--VAISNVKFVDFRGTSSNKNAITLKCSETTHCVDVVMDGIDITMANGG-K 370

[Close]

BLAST of MS023969 vs. TAIR 10
Match: AT5G27530.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 273.1 bits (697), Expect = 3.1e-73
Identity = 154/372 (41.40%), Postives = 217/372 (58.33%), Query Frame = 0


Query: 13  INFLSSWIVSASPSFNVLDYGAIGNGQADDS----------------------------- 72
           INF+   +V+    +NVL +GA G+GQ DDS                             
Sbjct: 40  INFICFGLVNGQ-IYNVLKFGAEGDGQTDDSNAFLQAWNATCGGEENINTLFIPSGKTYL 99

Query: 73  -----------------QLKGTLVAPKK-SSWSNDDRERWVQFSDIDGLIVNGTGQFDGQ 132
                            QL G +VAP   +SWSN   + W+ FS + GL+++G+G  +G+
Sbjct: 100 LQPIEFKGPCKSTSIKLQLDGIIVAPSNITSWSNPKSQTWISFSGVPGLMIDGSGTINGR 159

Query: 133 GSSWWEDCEHCQRPTALFFHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPED 192
           GSS+WE         AL   NCN L +  +  I+S K+HISI  C+ V IS ++I+APE+
Sbjct: 160 GSSFWE---------ALHICNCNNLTINGITSIDSPKSHISIKNCHYVAISKINILAPEN 219

Query: 193 SPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNR 252
           SPNTDGID+S S NV I +S + TGDDCIA+N GSS+I+I  V CGPGHGIS+GSLG + 
Sbjct: 220 SPNTDGIDISYSTNVNIFDSTIQTGDDCIAINTGSSSINITQVNCGPGHGISVGSLGADG 279

Query: 253 EYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTGL 312
           E   V +++V  C    T NG RIKTWQGG GYARNI+FE ITL+  +NPIIIDQ YT  
Sbjct: 280 ENAAVSDVYVTQCTFNKTTNGARIKTWQGGQGYARNISFENITLINVQNPIIIDQQYTDK 339

Query: 313 AKVQKNKLFDIKVSNVTYREVNGTSANENAIILNCSR-SRCSNIILENVSIKPTDPKMEA 337
             +   K   + +S+V Y    GT+ NE+AI+L CS  + C +++++++ +   + + + 
Sbjct: 340 VLLDATKDSAVAISSVKYVGFQGTTLNEDAIMLKCSAITYCKDMVIDDIEVTMENGE-KP 399

[Close]

BLAST of MS023969 vs. TAIR 10
Match: AT4G32375.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 270.0 bits (689), Expect = 2.6e-72
Identity = 137/306 (44.77%), Postives = 192/306 (62.75%), Query Frame = 0


Query: 44  QLKGTLVAPKK-SSWSNDDRERWVQFSDIDGLIVNGTGQFDGQGSSWWEDCEHCQRPTAL 103
           Q+ G L APK    W N + + W+ F+++ GL++NG+G     G +WW   EH  RP  +
Sbjct: 52  QIDGKLEAPKMVREWGNYESKCWLCFTNVKGLVLNGSGILHPHGEAWWSSIEHSHRPRTI 111

Query: 104 FFHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSRSNNVLI 163
            F   + +    +  +NS KNHISI  C +V +S+ H+IAP+DSPNTDGID++ SNN+ I
Sbjct: 112 GFSGSSNILYNGLTQMNSPKNHISILDCTNVTLSNFHLIAPKDSPNTDGIDIAHSNNIRI 171

Query: 164 QNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVKNCLLKG 223
            NS + TGDDCIA+N GS +I+I  V CGPGHGISIGSLG     + V+N+ +++C   G
Sbjct: 172 FNSSIQTGDDCIAINGGSYDINITHVACGPGHGISIGSLGRYSVNDTVQNVKIRHCSFNG 231

Query: 224 TQNGMRIKTW--QGGYGYARNITFEQITLMGAKNPIIIDQYY-TGLAKVQKNKLFDIKVS 283
           T+NG RIKTW  +GG G A+NI +E ITL   K PIIIDQ+Y  G     K  +  +KVS
Sbjct: 232 TENGARIKTWTVRGGLGVAKNILYENITLTDTKYPIIIDQHYCNGGHNCTKEAMTAVKVS 291

Query: 284 NVTYREVNGTSANENAIILNCSR-SRCSNIILENVSIKPTDPKMEAKAFCQNVDGRSTYA 343
           NVT+R   GT AN+ AI L+C   + C +I++E+++I  +  K    A+CQ  D  S + 
Sbjct: 292 NVTFRYFTGTCANDIAIKLDCDEVTGCKDIVMEHINITSSSTKRPLTAYCQFADIISHFV 351

Query: 344 VPTVPC 345
              + C
Sbjct: 352 SMKIKC 357

The following BLAST results are available for this feature:

BLAST of MS023969 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141107.1	1.6e-180	83.21	probable polygalacturonase At3g15720 [Momordica charantia]	[more]
XP_022141108.1	3.7e-161	73.28	probable polygalacturonase At3g15720 [Momordica charantia]	[more]
XP_038888688.1	1.7e-137	62.44	probable polygalacturonase At3g15720 [Benincasa hispida]	[more]
XP_038888676.1	4.1e-136	63.20	probable polygalacturonase At3g15720 [Benincasa hispida]	[more]
KGN53196.1	3.0e-134	62.56	hypothetical protein Csa_014667 [Cucumis sativus]	[more]

BLAST of MS023969 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LW07	2.8e-79	44.16	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
Q94AJ5	2.3e-65	40.89	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
Q8RY29	1.2e-61	43.52	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]
Q02096	2.9e-60	40.51	Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1	[more]
Q7M1E7	8.6e-60	36.58	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]

BLAST of MS023969 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CIX8	7.8e-181	83.21	probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A6J1CJJ6	1.8e-161	73.28	probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A0A0KZ95	1.4e-134	62.56	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1	[more]
A0A5D3CYS8	6.0e-133	61.93	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3BYT5	8.4e-127	60.71	probable polygalacturonase At3g15720 OS=Cucumis melo OX=3656 GN=LOC103494869 PE=...	[more]

BLAST of MS023969 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15720.1	2.0e-80	44.16	Pectin lyase-like superfamily protein	[more]
AT3G15720.2	4.2e-78	43.64	Pectin lyase-like superfamily protein	[more]
AT4G35670.1	8.7e-76	41.67	Pectin lyase-like superfamily protein	[more]
AT5G27530.1	3.1e-73	41.40	Pectin lyase-like superfamily protein	[more]
AT4G32375.1	2.6e-72	44.77	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 157..178 e-value: 270.0 score: 10.3 coord: 130..156 e-value: 110.0 score: 11.7 coord: 180..200 e-value: 6600.0 score: 1.4 coord: 239..260 e-value: 3300.0 score: 3.5 coord: 210..231 e-value: 89.0 score: 11.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 19..344 e-value: 7.4E-95 score: 320.3
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 44..332 e-value: 3.7E-83 score: 279.3
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 187..200
None	No IPR available	PANTHER	PTHR31375:SF232	SUBFAMILY NOT NAMED	coord: 43..345
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 43..345
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 12..345

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS023969.1	MS023969.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity