MS023844 (gene) Bitter gourd (TR) v1

BLAST of MS023844 vs. NCBI nr
Match: XP_022155007.1 (probable calcium-binding protein CML21 [Momordica charantia])

HSP 1 Score: 449.9 bits (1156), Expect = 1.3e-122
Identity = 227/228 (99.56%), Postives = 228/228 (100.00%), Query Frame = 0

Query: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60
           MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS
Sbjct: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60

Query: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120
           TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL
Sbjct: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120

Query: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAI+ETTSG
Sbjct: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAIHETTSG 180

Query: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 229
           ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE
Sbjct: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 228

BLAST of MS023844 vs. NCBI nr
Match: XP_004149571.1 (probable calcium-binding protein CML21 [Cucumis sativus] >KGN56046.1 hypothetical protein Csa_009502 [Cucumis sativus])

HSP 1 Score: 407.1 bits (1045), Expect = 9.7e-110
Identity = 205/228 (89.91%), Postives = 215/228 (94.30%), Query Frame = 0

Query: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60
           MGS VGK ESPR+CVPETKLEAKMVE M QRA+KGSIIRSFD IILKFPKIDDS RNC +
Sbjct: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60

Query: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120
            FQQFDED NGIIDRRELKKCFDGLEILLTEEEIDDLF+ACDISTAMG+KFNEFIVLL L
Sbjct: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120

Query: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           VYLL+DDP  AVF+KSQFGMPKLE+TFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG
Sbjct: 121 VYLLKDDPN-AVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180

Query: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 229
           ERSSGRIAM+RFEEMDWDKNGMVNFKEFLFAFTRWVG+ ENEDGEEEE
Sbjct: 181 ERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 227

BLAST of MS023844 vs. NCBI nr
Match: XP_008449066.1 (PREDICTED: probable calcium-binding protein CML21 [Cucumis melo])

HSP 1 Score: 395.6 bits (1015), Expect = 2.9e-106
Identity = 198/225 (88.00%), Postives = 210/225 (93.33%), Query Frame = 0

Query: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60
           MGS VGK ESPR+CVPETKLEAKMVE M +RA+KGSIIRSFD I+LKFPKIDDS RNC +
Sbjct: 1   MGSVVGKLESPRECVPETKLEAKMVETMRRRATKGSIIRSFDCILLKFPKIDDSLRNCKT 60

Query: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120
            FQQFDED NGIIDRRELKKCFDGLEILLTEEEIDDLFEACDIS AMG+KFNEFIVLL L
Sbjct: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISAAMGIKFNEFIVLLCL 120

Query: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           VYLL+DDP  AVF+KSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEM+SAINETTSG
Sbjct: 121 VYLLKDDPD-AVFSKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMISAINETTSG 180

Query: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGE 226
           ERSSGRIAM+RFEEMDWDKNGMVNFKEFLFAFTRWVG+ ENE+ E
Sbjct: 181 ERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEEEE 224

BLAST of MS023844 vs. NCBI nr
Match: XP_038905190.1 (probable calcium-binding protein CML21 [Benincasa hispida] >XP_038905191.1 probable calcium-binding protein CML21 [Benincasa hispida] >XP_038905192.1 probable calcium-binding protein CML21 [Benincasa hispida] >XP_038905193.1 probable calcium-binding protein CML21 [Benincasa hispida])

HSP 1 Score: 394.0 bits (1011), Expect = 8.5e-106
Identity = 201/228 (88.16%), Postives = 210/228 (92.11%), Query Frame = 0

Query: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60
           MGS VGK ESP DCVPETKLEAKMVEAM  RASKGSIIRSFDSIILKFPKIDDS R C +
Sbjct: 1   MGSVVGKLESP-DCVPETKLEAKMVEAMRHRASKGSIIRSFDSIILKFPKIDDSLRKCKT 60

Query: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120
            FQQFDED NGIIDRRELK+CFDGLEI LTEEEIDDLF+ACDISTAMG+KFNEFIVLL L
Sbjct: 61  IFQQFDEDLNGIIDRRELKECFDGLEISLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120

Query: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           VYLL+DDP A  F+KSQFGMPKLEETFESLVDAF FLDKNKDGYVSKSEM+SAINETTSG
Sbjct: 121 VYLLKDDPNAK-FSKSQFGMPKLEETFESLVDAFAFLDKNKDGYVSKSEMISAINETTSG 180

Query: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 229
           ERSSGRIAM+RFEEMDWDKNGMVNFKEFLFAFT WVG+ ENEDGEEEE
Sbjct: 181 ERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTHWVGIDENEDGEEEE 226

BLAST of MS023844 vs. NCBI nr
Match: XP_023540519.1 (probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo] >XP_023540520.1 probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo] >XP_023540521.1 probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo] >XP_023540522.1 probable calcium-binding protein CML21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 382.5 bits (981), Expect = 2.6e-102
Identity = 193/228 (84.65%), Postives = 206/228 (90.35%), Query Frame = 0

Query: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60
           MGS VGK ES  +CVPETKLEAKMVEAM QRASKGSIIRSFD IILKFPKID S R C +
Sbjct: 1   MGSVVGKLESHSECVPETKLEAKMVEAMRQRASKGSIIRSFDCIILKFPKIDGSLRKCKT 60

Query: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120
            F++FDEDSNGIIDRRELK+CFDGLEI LT+EEIDDLF+ACD+STAMG+KFNEFIVLL L
Sbjct: 61  IFEKFDEDSNGIIDRRELKECFDGLEISLTQEEIDDLFDACDMSTAMGIKFNEFIVLLCL 120

Query: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           VYLL+DDP      KSQFGMPKLEETFESLVDAFVFLD NKDGYVSKSEM+SAINETTSG
Sbjct: 121 VYLLKDDPN----HKSQFGMPKLEETFESLVDAFVFLDANKDGYVSKSEMISAINETTSG 180

Query: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 229
           ERSSGRI M+RFEEMDWDKNGMVNFKEFLFAFTRWVG+ ENEDGEEEE
Sbjct: 181 ERSSGRIGMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 224

BLAST of MS023844 vs. ExPASy Swiss-Prot
Match: Q52K82 (Probable calcium-binding protein CML21 OS=Arabidopsis thaliana OX=3702 GN=CML21 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 9.8e-81
Identity = 156/229 (68.12%), Postives = 187/229 (81.66%), Query Frame = 0

Query: 1   MGSAVGKYES-PRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCI 60
           MG AV K E+  ++ VPETKLEAK++EA+ +RAS+G+ ++SF+SI+LKFPKIDD  RNC 
Sbjct: 1   MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCK 60

Query: 61  STFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLS 120
           + FQ+FDEDSNG ID  ELK C   LEI   EEEI+DLF+ACDI+  MG+ F EFIVLL 
Sbjct: 61  AIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLC 120

Query: 121 LVYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTS 180
           LVYLL+DD ++ +  K   GMPKLE TFE+LVD FVFLD+NKDGYVS+ EMV AI+E  S
Sbjct: 121 LVYLLKDD-SSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDE--S 180

Query: 181 GERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 229
           GERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFT+WVG+ ENE+ EEE+
Sbjct: 181 GERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTQWVGIDENEEEEEED 226

BLAST of MS023844 vs. ExPASy Swiss-Prot
Match: Q9LRN6 (Probable calcium-binding protein CML22 OS=Arabidopsis thaliana OX=3702 GN=CML22 PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 3.1e-42
Identity = 88/208 (42.31%), Postives = 131/208 (62.98%), Query Frame = 0

Query: 17  ETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCISTFQQFDEDSNGIIDRR 76
           + KL  KMVE+        S ++S DSII+KFPK+ +  RN  S F+ +D D+NG ID  
Sbjct: 18  DAKLARKMVESRSIYPGHRS-LKSMDSIIMKFPKLREGLRNIRSVFESYDNDTNGTIDIE 77

Query: 77  ELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSLVYLLRDDPTAAVFAKS 136
           ELKKC + L++ L++EE+  L+  CD+  + G++FNEFIVLL L+YLL    + +     
Sbjct: 78  ELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCLIYLLAKPSSESSTESR 137

Query: 137 QFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAI-NETTSGERSSGRIAMKRFEEM 196
           + G   +E  F+ +V+ F+FLDK+  G ++K++++  + NE    ERS   +   RFEEM
Sbjct: 138 EMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYPLERSPSHVTNMRFEEM 197

Query: 197 DWDKNGMVNFKEFLFAFTRWVGLGENED 224
           DW + G V F+EFLFAF  WVGL + +D
Sbjct: 198 DWGRKGKVGFREFLFAFMSWVGLDDADD 224

BLAST of MS023844 vs. ExPASy Swiss-Prot
Match: Q7XSQ5 (Calcium-dependent protein kinase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK12 PE=2 SV=2)

HSP 1 Score: 58.2 bits (139), Expect = 1.5e-07
Identity = 39/148 (26.35%), Postives = 71/148 (47.97%), Query Frame = 0

Query: 62  FQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSLV 121
           F   D D +G I   ELK     L   ++E E+  L EA D+  +  + ++EF+      
Sbjct: 400 FNNMDTDRSGTITVEELKVGLTKLGSRISEAEVQKLMEAVDVDKSGSIDYSEFLT----- 459

Query: 122 YLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 181
                         +     KLE+  E L+ AF   DK+  GY+++ E+  A+ E   G+
Sbjct: 460 --------------AMINKHKLEKE-EDLLRAFQHFDKDNSGYITRDELEQAMAEYGMGD 519

Query: 182 RSSGRIAMKRFEEMDWDKNGMVNFKEFL 210
            ++ +  +   +E+D DK+G ++++EF+
Sbjct: 520 EANIKQVL---DEVDKDKDGRIDYEEFV 524

BLAST of MS023844 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.5e-07
Identity = 40/147 (27.21%), Postives = 69/147 (46.94%), Query Frame = 0

Query: 62  FQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSLV 121
           FQ FD++ +G I + ELK  F  + I++ E EI+++    D++    +  +EF  L    
Sbjct: 10  FQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQ-- 69

Query: 122 YLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 181
                             M + +E  E + +AF   D+N DG+++  E+ S +      +
Sbjct: 70  -----------------EMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQ 129

Query: 182 RSSGRIAMKRFEEMDWDKNGMVNFKEF 209
             +     K   ++D D +GMVNFKEF
Sbjct: 130 GRTLEDCKKMISKVDVDGDGMVNFKEF 137

BLAST of MS023844 vs. ExPASy Swiss-Prot
Match: P49101 (Calcium-dependent protein kinase 2 OS=Zea mays OX=4577 GN=CPK2 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.9e-07
Identity = 42/157 (26.75%), Postives = 69/157 (43.95%), Query Frame = 0

Query: 52  DDSFRNCISTFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKF 111
           ++  +     F   D D++G I   ELK     L   L+E E+  L EA D+     + +
Sbjct: 365 EEEIKGLKQMFMNMDTDNSGTITYEELKAGLAKLGSKLSEAEVKQLMEAADVDGNGSIDY 424

Query: 112 NEFIVLLSLVYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMV 171
            EFI      + L  D                    E L  AF + DK+  G++++ E+ 
Sbjct: 425 VEFITATMHRHKLERD--------------------EHLFKAFQYFDKDNSGFITRDELE 484

Query: 172 SAINETTSGERSSGRIAMKRFEEMDWDKNGMVNFKEF 209
           SA+ E   G+ S+ R   +   E+D D +G +N++EF
Sbjct: 485 SALIEHEMGDTSTIR---EIISEVDTDNDGRINYEEF 498

BLAST of MS023844 vs. ExPASy TrEMBL
Match: A0A6J1DN80 (probable calcium-binding protein CML21 OS=Momordica charantia OX=3673 GN=LOC111022149 PE=4 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 6.3e-123
Identity = 227/228 (99.56%), Postives = 228/228 (100.00%), Query Frame = 0

Query: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60
           MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS
Sbjct: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60

Query: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120
           TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL
Sbjct: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120

Query: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAI+ETTSG
Sbjct: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAIHETTSG 180

Query: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 229
           ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE
Sbjct: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 228

BLAST of MS023844 vs. ExPASy TrEMBL
Match: A0A0A0L7G7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G061000 PE=4 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 4.7e-110
Identity = 205/228 (89.91%), Postives = 215/228 (94.30%), Query Frame = 0

Query: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60
           MGS VGK ESPR+CVPETKLEAKMVE M QRA+KGSIIRSFD IILKFPKIDDS RNC +
Sbjct: 1   MGSVVGKLESPRECVPETKLEAKMVETMKQRATKGSIIRSFDCIILKFPKIDDSLRNCKT 60

Query: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120
            FQQFDED NGIIDRRELKKCFDGLEILLTEEEIDDLF+ACDISTAMG+KFNEFIVLL L
Sbjct: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFDACDISTAMGMKFNEFIVLLCL 120

Query: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           VYLL+DDP  AVF+KSQFGMPKLE+TFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG
Sbjct: 121 VYLLKDDPN-AVFSKSQFGMPKLEQTFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180

Query: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 229
           ERSSGRIAM+RFEEMDWDKNGMVNFKEFLFAFTRWVG+ ENEDGEEEE
Sbjct: 181 ERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 227

BLAST of MS023844 vs. ExPASy TrEMBL
Match: A0A1S3BLU5 (probable calcium-binding protein CML21 OS=Cucumis melo OX=3656 GN=LOC103491047 PE=4 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.4e-106
Identity = 198/225 (88.00%), Postives = 210/225 (93.33%), Query Frame = 0

Query: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60
           MGS VGK ESPR+CVPETKLEAKMVE M +RA+KGSIIRSFD I+LKFPKIDDS RNC +
Sbjct: 1   MGSVVGKLESPRECVPETKLEAKMVETMRRRATKGSIIRSFDCILLKFPKIDDSLRNCKT 60

Query: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120
            FQQFDED NGIIDRRELKKCFDGLEILLTEEEIDDLFEACDIS AMG+KFNEFIVLL L
Sbjct: 61  IFQQFDEDLNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISAAMGIKFNEFIVLLCL 120

Query: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           VYLL+DDP  AVF+KSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEM+SAINETTSG
Sbjct: 121 VYLLKDDPD-AVFSKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMISAINETTSG 180

Query: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGE 226
           ERSSGRIAM+RFEEMDWDKNGMVNFKEFLFAFTRWVG+ ENE+ E
Sbjct: 181 ERSSGRIAMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEEEE 224

BLAST of MS023844 vs. ExPASy TrEMBL
Match: A0A6J1KV85 (probable calcium-binding protein CML21 OS=Cucurbita maxima OX=3661 GN=LOC111498495 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.8e-102
Identity = 193/228 (84.65%), Postives = 206/228 (90.35%), Query Frame = 0

Query: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60
           MGS VGK ES  +CVPETKLEAKMVEA+ QRASKGSIIRSFD IILKFPKID S R C +
Sbjct: 1   MGSVVGKLESHSECVPETKLEAKMVEAIRQRASKGSIIRSFDCIILKFPKIDGSLRKCKT 60

Query: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120
            F++FDEDSNGIIDRRELK+CFDGLEI LT+EEIDDLF+ACD+STAMG+KFNEFIVLL L
Sbjct: 61  IFEKFDEDSNGIIDRRELKECFDGLEISLTQEEIDDLFDACDMSTAMGIKFNEFIVLLCL 120

Query: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           VYLL+DDP      KSQFGMPKLEETFESLVDAFVFLD NKDGYVSKSEMVSAINETTSG
Sbjct: 121 VYLLKDDPN----HKSQFGMPKLEETFESLVDAFVFLDTNKDGYVSKSEMVSAINETTSG 180

Query: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 229
           ERSSGRI M+RFEEMDWDKNGMVNFKEFLFAFTRWVG+ ENEDGEEEE
Sbjct: 181 ERSSGRIGMRRFEEMDWDKNGMVNFKEFLFAFTRWVGIDENEDGEEEE 224

BLAST of MS023844 vs. ExPASy TrEMBL
Match: A0A6J1G727 (probable calcium-binding protein CML21 OS=Cucurbita moschata OX=3662 GN=LOC111451446 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.6e-102
Identity = 192/228 (84.21%), Postives = 206/228 (90.35%), Query Frame = 0

Query: 1   MGSAVGKYESPRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCIS 60
           MGS VGK ES R+CVPETKLEAKMVEA+ QRASKGSIIRSFD IILKFPKID S R C +
Sbjct: 1   MGSVVGKLESQRECVPETKLEAKMVEAIRQRASKGSIIRSFDCIILKFPKIDASLRKCKT 60

Query: 61  TFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSL 120
            F++FDEDSNGIIDRRELK+CFDGLEI LT+EEIDDLF+ACD+STAMG+KFNEFIVLL L
Sbjct: 61  IFEKFDEDSNGIIDRRELKECFDGLEISLTQEEIDDLFDACDMSTAMGIKFNEFIVLLCL 120

Query: 121 VYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSG 180
           VYLL+DDP      KSQFGMPKLEETFESLVDAFVFLD NKDGYVSKSEM+SAINETTSG
Sbjct: 121 VYLLKDDPN----HKSQFGMPKLEETFESLVDAFVFLDTNKDGYVSKSEMISAINETTSG 180

Query: 181 ERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 229
           ERSSGRI M+RFEEMDWDKNGMVN KEFLFAFTRWVG+ ENEDGEEEE
Sbjct: 181 ERSSGRIGMRRFEEMDWDKNGMVNIKEFLFAFTRWVGIDENEDGEEEE 224

BLAST of MS023844 vs. TAIR 10
Match: AT4G26470.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 301.2 bits (770), Expect = 7.0e-82
Identity = 156/229 (68.12%), Postives = 187/229 (81.66%), Query Frame = 0

Query: 1   MGSAVGKYES-PRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCI 60
           MG AV K E+  ++ VPETKLEAK++EA+ +RAS+G+ ++SF+SI+LKFPKIDD  RNC 
Sbjct: 1   MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCK 60

Query: 61  STFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLS 120
           + FQ+FDEDSNG ID  ELK C   LEI   EEEI+DLF+ACDI+  MG+ F EFIVLL 
Sbjct: 61  AIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLC 120

Query: 121 LVYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTS 180
           LVYLL+DD ++ +  K   GMPKLE TFE+LVD FVFLD+NKDGYVS+ EMV AI+E  S
Sbjct: 121 LVYLLKDD-SSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDE--S 180

Query: 181 GERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTRWVGLGENEDGEEEE 229
           GERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFT+WVG+ ENE+ EEE+
Sbjct: 181 GERSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTQWVGIDENEEEEEED 226

BLAST of MS023844 vs. TAIR 10
Match: AT4G26470.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 235.7 bits (600), Expect = 3.6e-62
Identity = 126/193 (65.28%), Postives = 153/193 (79.27%), Query Frame = 0

Query: 1   MGSAVGKYES-PRDCVPETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCI 60
           MG AV K E+  ++ VPETKLEAK++EA+ +RAS+G+ ++SF+SI+LKFPKIDD  RNC 
Sbjct: 1   MGGAVTKSETLQKEWVPETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCK 60

Query: 61  STFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLS 120
           + FQ+FDEDSNG ID  ELK C   LEI   EEEI+DLF+ACDI+  MG+ F EFIVLL 
Sbjct: 61  AIFQEFDEDSNGSIDHTELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLC 120

Query: 121 LVYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTS 180
           LVYLL+DD ++ +  K   GMPKLE TFE+LVD FVFLD+NKDGYVS+ EMV AI+E  S
Sbjct: 121 LVYLLKDD-SSTLQKKWTMGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDE--S 180

Query: 181 GERSSGRIAMKRF 193
           GERSSGRIAMKRF
Sbjct: 181 GERSSGRIAMKRF 190

BLAST of MS023844 vs. TAIR 10
Match: AT3G24110.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 173.3 bits (438), Expect = 2.2e-43
Identity = 88/208 (42.31%), Postives = 131/208 (62.98%), Query Frame = 0

Query: 17  ETKLEAKMVEAMIQRASKGSIIRSFDSIILKFPKIDDSFRNCISTFQQFDEDSNGIIDRR 76
           + KL  KMVE+        S ++S DSII+KFPK+ +  RN  S F+ +D D+NG ID  
Sbjct: 18  DAKLARKMVESRSIYPGHRS-LKSMDSIIMKFPKLREGLRNIRSVFESYDNDTNGTIDIE 77

Query: 77  ELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSLVYLLRDDPTAAVFAKS 136
           ELKKC + L++ L++EE+  L+  CD+  + G++FNEFIVLL L+YLL    + +     
Sbjct: 78  ELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCLIYLLAKPSSESSTESR 137

Query: 137 QFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAI-NETTSGERSSGRIAMKRFEEM 196
           + G   +E  F+ +V+ F+FLDK+  G ++K++++  + NE    ERS   +   RFEEM
Sbjct: 138 EMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYPLERSPSHVTNMRFEEM 197

Query: 197 DWDKNGMVNFKEFLFAFTRWVGLGENED 224
           DW + G V F+EFLFAF  WVGL + +D
Sbjct: 198 DWGRKGKVGFREFLFAFMSWVGLDDADD 224

BLAST of MS023844 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 58.2 bits (139), Expect = 1.0e-08
Identity = 40/147 (27.21%), Postives = 69/147 (46.94%), Query Frame = 0

Query: 62  FQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKFNEFIVLLSLV 121
           FQ FD++ +G I + ELK  F  + I++ E EI+++    D++    +  +EF  L    
Sbjct: 10  FQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQ-- 69

Query: 122 YLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMVSAINETTSGE 181
                             M + +E  E + +AF   D+N DG+++  E+ S +      +
Sbjct: 70  -----------------EMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQ 129

Query: 182 RSSGRIAMKRFEEMDWDKNGMVNFKEF 209
             +     K   ++D D +GMVNFKEF
Sbjct: 130 GRTLEDCKKMISKVDVDGDGMVNFKEF 137

BLAST of MS023844 vs. TAIR 10
Match: AT1G76040.2 (calcium-dependent protein kinase 29 )

HSP 1 Score: 57.4 bits (137), Expect = 1.8e-08
Identity = 38/158 (24.05%), Postives = 74/158 (46.84%), Query Frame = 0

Query: 52  DDSFRNCISTFQQFDEDSNGIIDRRELKKCFDGLEILLTEEEIDDLFEACDISTAMGVKF 111
           ++  +    TF+  D D +G I   EL+     L   LTE EI  L EA D+  +  + +
Sbjct: 411 EEEIKGLKQTFKNMDTDESGTITFDELRNGLHRLGSKLTESEIKQLMEAADVDKSGTIDY 470

Query: 112 NEFIVLLSLVYLLRDDPTAAVFAKSQFGMPKLEETFESLVDAFVFLDKNKDGYVSKSEMV 171
            EF+      + L                    E  E+L++AF + DK++ G++++ E+ 
Sbjct: 471 IEFVTATMHRHRL--------------------EKEENLIEAFKYFDKDRSGFITRDELK 530

Query: 172 SAINETTSGERSSGRIAMKRFEEMDWDKNGMVNFKEFL 210
            ++ E   G+ ++     +   ++D D +G +N++EF+
Sbjct: 531 HSMTEYGMGDDAT---IDEVINDVDTDNDGRINYEEFV 545