MS023668 (gene) Bitter gourd (TR) v1

Overview


Name	MS023668
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Mediator of RNA polymerase II transcription subunit 20
Location	scaffold570: 157340 .. 159403 (+)
RNA-Seq Expression	MS023668
Synteny	MS023668

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGGCTAGGGTTTTGCATTGGCAACCGAATGCCAGCAGCACTGTTAACAGTCAGATCCTCAACGAAGTTTCTCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAACCTACTCACCTTTTACAGACCCATCTTACGAGGTAATTGCACTCTTTTCATAGAGTCGTTTGTAAGAATATCTTCTTTTTTCCTTTTTCTCGAGAGGGGGCTGTGGCCGTCGGGATTTAATTACCCGAATGAATCTTAAATCTGTCAGCAGACCAAAACTTACCGGCGGCCGAGTTCCCGCGCGACTTTCTCAGAATCTCGCTGCCGGAGCAACCAAACAAGTATTATCTTATAATCCGTGGCCAACGGATCGTCTTAGAAGCTGATTTTACGATTCAAACTATAATGGAGAATTTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGTTCAACACCAAACTTTTCTAGTTCTCATGTAAACTGCACTTTCCCATGTCCAGAAATTCAAAATTGAAGTTGATTTTTGTGGCTTTCTGGTGTCTGATCTTTTGCCCTTTTGTTTTAGTTCGTGTGAGATCGATAAATCTTTGGTTACAAACAGTTTACCTTATTATTTTTTAGGGGTTCCAATACCAATTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAAGCATTCTGAGAATTTGAGAGGAATAGTTATGGAGGTAAGGCTCATTTCATCATTTCATGTCAACTCTGTTCTTGTGTCTTAACAATTGCATGCTCTTCTTATTGGTTTTTTATGTCAATATTCTTTATCTGAGCGAGCAGTTTCTTTCCCATGGGCTGTGTCAAAACAATGTTTGGTACAGGAAGTAGTGATTCAACTTATCTTAATGCTTATGGCCTCTGTTGTTCTCATTTTGATAAACAATGAAGTGGAAGAGAAAGGCCAATCATCTGATAGAAGTATCAACTATCATTTTCTCTGAAAAGTTTGTGTAAGGTCGCGATCTTATGTTGAGAAGTAGTGAGATCTAGTTAGGTTAGGATTTCCTTGCAAGCCTGTGTAAGAGTAGTCATGAATCAATTAATTGCTCTGAAATCGTTGATTTTATTCAGTTTGGAAAGTGTCTTTCAACTCATTTGGATGCTTGGAAATAGTGTTTGGTCTTTGCTTGCGCTCGACTTCATTGTGGTGCTCATCCTTGAACAAAATAAAGTGAGATAGACTGCCTATCACCTTTCAATCATCTGCTCTTGTTATCCTCTTTCCCCTTCCATATCGTTCTTCAGAAACACATTGCCATGTTCTTTATTGTATTGTCAAGTCCAAGTCCAAGTCCAAATAGGATATGCTAGCAACTAAGCAAGTGTGATGAAGCCTCCCTCTGAGATGGATTGCTCGCATGCTGAAGTTGGAAACCTGAAACTTCTCTAGGAAATTTAAAGTTCAGTTCTGTAATTTGGTTTTAACCTACTTTGATGTATATCATATTGGATGTATTGTATTGTTAGTTCTGGGAAGTAAGAATCATATTTAAGTGGGATTAATTAGAAACTAGAATCTCTGTCAAATGTGTGCACATTTGATTTTTGTGCTGTAATTACGAAAATGGCTTTTTGCTGCTGTTCCAATTGACGACCATTTAGAACAGTTGAGACCATATATTGCTCGTGTCGCAGTTTTTTCTTTTCTTTTTTTTTTCCTTTTTGAGAAATGAAGCGTGGGATTCAAACTCATGATCTTTTGGTCAGAGAATGTGCCAATTTCGCTCATGTTGGCACTTGTGTCGTAGTTATGCAATAATGTACTAAATCCTGTATATTGCAGATGGAGTATCTCCCAATTTCTTCAATGGAGAAATCAAGACAGGTGATGGGGGGATTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCACTGCCAGGTCGTTTCATGCACATAGAACCGAACTTTGCAGAATATGGACTGTTGGATTACTACACTCCTCAACACACAGCCGTTCAGTATGCCAATGTCATGGCTCAACTAATCGGAACAGTTCAAGCAGTTCAATCAGTGAGAAAT

mRNA sequence

TTGGCTAGGGTTTTGCATTGGCAACCGAATGCCAGCAGCACTGTTAACAGTCAGATCCTCAACGAAGTTTCTCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAACCTACTACCTTTTACAGACCCATCTTACGAGCAGACCAAAACTTACCGGCGGCCGAGTTCCCGCGCGACTTTCTCAGAATCTCGCTGCCGGAGCAACCAAACAAGTATTATCTTATAATCCGTGGCCAACGGATCGTCTTAGAAGCTGATTTTACGATTCAAACTATAATGGAGAATTTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAATACCAATTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAAGCATTCTGAGAATTTGAGAGGAATAGTTATGGAGATGGAGTATCTCCCAATTTCTTCAATGGAGAAATCAAGACAGGTGATGGGGGGATTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCACTGCCAGGTCGTTTCATGCACATAGAACCGAACTTTGCAGAATATGGACTGTTGGATTACTACACTCCTCAACACACAGCCGTTCAGTATGCCAATGTCATGGCTCAACTAATCGGAACAGTTCAAGCAGTTCAATCAGTGAGAAAT

Coding sequence (CDS)

TTGGCTAGGGTTTTGCATTGGCAACCGAATGCCAGCAGCACTGTTAACAGTCAGATCCTCAACGAAGTTTCTCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAACCTACTACCTTTTACAGACCCATCTTACGAGCAGACCAAAACTTACCGGCGGCCGAGTTCCCGCGCGACTTTCTCAGAATCTCGCTGCCGGAGCAACCAAACAAGTATTATCTTATAATCCGTGGCCAACGGATCGTCTTAGAAGCTGATTTTACGATTCAAACTATAATGGAGAATTTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAATACCAATTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAAGCATTCTGAGAATTTGAGAGGAATAGTTATGGAGATGGAGTATCTCCCAATTTCTTCAATGGAGAAATCAAGACAGGTGATGGGGGGATTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCACTGCCAGGTCGTTTCATGCACATAGAACCGAACTTTGCAGAATATGGACTGTTGGATTACTACACTCCTCAACACACAGCCGTTCAGTATGCCAATGTCATGGCTCAACTAATCGGAACAGTTCAAGCAGTTCAATCAGTGAGAAAT

Protein sequence

LARVLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPTTFYRPILRADQNLPAAEFPRDFLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQSVRN

Homology

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BLAST of MS023668 vs. NCBI nr
Match: XP_038889134.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Benincasa hispida])

HSP 1 Score: 396.4 bits (1017), Expect = 1.7e-106
Identity = 201/219 (91.78%), Postives = 205/219 (93.61%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           VLHWQPNA STVNSQILNEVSQCVESIHGVKGGKWK T TFY+PILR DQNLPAAEFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNLPAAEFPRD 65

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           FL ISLPEQPNKYYLIIRGQRIVLEADFTIQ IME LQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP +SENLRGIVME+EYLPISSMEKSR+VM  FFEVWQEAMSKSSLPGRFMHIEP
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRKVMEEFFEVWQEAMSKSSLPGRFMHIEP 185

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQSVRN 222
           NFAEYGL DYYT QHTAVQYANVMAQLI TVQAVQS RN
Sbjct: 186 NFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023668 vs. NCBI nr
Match: XP_008459711.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo])

HSP 1 Score: 391.3 bits (1004), Expect = 5.4e-105
Identity = 197/219 (89.95%), Postives = 203/219 (92.69%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           VLHWQPNA STVNSQILNEVSQCVESIHGVKGGKWK T TFY+PILR DQN PAAEFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNSPAAEFPRD 65

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           FL ISLPEQPNKYYLIIRGQRIVLEADFTIQ IME LQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP +SENLRGIVME+EYLPISSMEKSRQ+M  FF+VWQEAMSKSSLPGRFMHIE 
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIES 185

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQSVRN 222
           NFAEYGL D+YTPQHTAVQY NVMAQLI TVQAVQS RN
Sbjct: 186 NFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023668 vs. NCBI nr
Match: KAA0061469.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo var. makuwa] >TYK10805.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 391.3 bits (1004), Expect = 5.4e-105
Identity = 197/219 (89.95%), Postives = 203/219 (92.69%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           VLHWQPNA STVNSQILNEVSQCVESIHGVKGGKWK T TFY+PILR DQN PAAEFPRD
Sbjct: 54  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNSPAAEFPRD 113

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           FL ISLPEQPNKYYLIIRGQRIVLEADFTIQ IME LQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 114 FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 173

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP +SENLRGIVME+EYLPISSMEKSRQ+M  FF+VWQEAMSKSSLPGRFMHIE 
Sbjct: 174 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIES 233

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQSVRN 222
           NFAEYGL D+YTPQHTAVQY NVMAQLI TVQAVQS RN
Sbjct: 234 NFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 271

[Close]

BLAST of MS023668 vs. NCBI nr
Match: XP_004141069.1 (mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60509.1 hypothetical protein Csa_019466 [Cucumis sativus])

HSP 1 Score: 389.8 bits (1000), Expect = 1.6e-104
Identity = 198/219 (90.41%), Postives = 202/219 (92.24%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           VLHWQPNA STVNSQILNEVSQCVESIHGVKGGKWK T TFY+PILR DQN PAAEFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNSPAAEFPRD 65

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           FL ISLPEQPNKYYLIIRGQRIVLEADFTIQ IME LQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP +SENLRGIVME+EYLPISSMEKSRQVM  FF+VWQEAMSKSSLPGRFMHIE 
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFMHIES 185

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQSVRN 222
           NFAEYGL DYYT QHTAVQY NVMAQLI TVQAVQS RN
Sbjct: 186 NFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023668 vs. NCBI nr
Match: XP_022946745.1 (mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata] >XP_022999230.1 mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita maxima] >XP_023546929.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546930.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6599532.1 Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 387.9 bits (995), Expect = 5.9e-104
Identity = 196/219 (89.50%), Postives = 202/219 (92.24%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           VLHWQPNA STVNSQILNEVSQCVESIHGVKGGKWK T TFY+PILR D N+ A EFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DTNIAAGEFPRD 65

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           FL IS+PEQPNKYYLIIRGQRIVLEADFTIQ IME LQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP +SENLRGIVME+EYLPISSMEKS+QVM  FFEVWQEAMSKSSLPGRFMHIEP
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEP 185

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQSVRN 222
           NFAEYGL DYYT QHTAVQYANVMAQLI TVQAVQS RN
Sbjct: 186 NFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023668 vs. ExPASy Swiss-Prot
Match: Q6NPF4 (Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana OX=3702 GN=MED20A PE=1 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 9.5e-81
Identity = 150/215 (69.77%), Postives = 179/215 (83.26%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           VLHWQPN  STV+SQILNE +QCVESI+GVK G+WK T  +Y+P+L+   N    EFPRD
Sbjct: 6   VLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKDQAN--QLEFPRD 65

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           FL ISL +QPNKYY IIR QRIVLEAD +IQ IME LQSYK +VAL F+GFQYQLGDF+L
Sbjct: 66  FLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLGDFRL 125

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP HSEN+RGIVME+EYLPISSMEK+++VM  F E+W EA++K SLPG+F++I+ 
Sbjct: 126 RVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFVNIDL 185

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQ 218
           NF E+GL D YTPQHTAV+YA VMA +I TVQAV+
Sbjct: 186 NFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

[Close]

BLAST of MS023668 vs. ExPASy Swiss-Prot
Match: Q9M0S0 (Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana OX=3702 GN=MED20B PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 1.4e-71
Identity = 131/213 (61.50%), Postives = 170/213 (79.81%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           +LHWQPN  ST +SQILNEV+Q +ES++GVK G WK T  +Y+P+L+   N   AEFPR+
Sbjct: 6   LLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQAN--QAEFPRE 65

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           F+ ISLPE+P+KYY +IR QRIV+EAD +IQ IME+LQSYK +++  FEG +YQLGDF+L
Sbjct: 66  FVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQLGDFRL 125

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP H+E +RG+VME+EYLPISSM  ++++M  F E+WQEAMSK SLPG+F++ E 
Sbjct: 126 RVGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFVNKEL 185

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQA 216
           NF ++GL D YTPQHTAV YA  MA L+  +QA
Sbjct: 186 NFEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

[Close]

BLAST of MS023668 vs. ExPASy Swiss-Prot
Match: Q54FL3 (Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium discoideum OX=44689 GN=med20 PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 3.5e-06
Identity = 45/158 (28.48%), Postives = 73/158 (46.20%), Query Frame = 0


Query: 60  PRDFLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGD 119
           PR+F  +   E+P K + + R    ++E D  + +I+E   SYK R        +Y+LGD
Sbjct: 50  PREFHLLLYDEKPKKCFTVCRD--TIVETDREMVSILEKTNSYKKRQTTEALISKYELGD 109

Query: 120 FQLRVGKVVPKHSENLRGIVMEMEY----LPISSMEKSRQV--------MGGFFEVWQEA 179
           F +RVG +V      +RGI++E+EY     P++S     ++        +G F E  Q +
Sbjct: 110 FIIRVGPIV--FRTEIRGILVEVEYTACSTPVTSPFNCTKLLSEFINGNLGPFPEPIQTS 169

Query: 180 MSKSSLPGRFMHIEPNFAEYGLLDYYTPQHTAVQYANV 206
              S+ P              L   Y+  HT+ QY N+
Sbjct: 170 FDFSAYP-------------TLPKSYSDLHTSYQYVNL 190

[Close]

BLAST of MS023668 vs. ExPASy TrEMBL
Match: A0A5D3CH02 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. makuwa OX=1194695 GN=MED20 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.6e-105
Identity = 197/219 (89.95%), Postives = 203/219 (92.69%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           VLHWQPNA STVNSQILNEVSQCVESIHGVKGGKWK T TFY+PILR DQN PAAEFPRD
Sbjct: 54  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNSPAAEFPRD 113

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           FL ISLPEQPNKYYLIIRGQRIVLEADFTIQ IME LQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 114 FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 173

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP +SENLRGIVME+EYLPISSMEKSRQ+M  FF+VWQEAMSKSSLPGRFMHIE 
Sbjct: 174 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIES 233

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQSVRN 222
           NFAEYGL D+YTPQHTAVQY NVMAQLI TVQAVQS RN
Sbjct: 234 NFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 271

[Close]

BLAST of MS023668 vs. ExPASy TrEMBL
Match: A0A1S3CAW6 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 GN=LOC103498750 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.6e-105
Identity = 197/219 (89.95%), Postives = 203/219 (92.69%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           VLHWQPNA STVNSQILNEVSQCVESIHGVKGGKWK T TFY+PILR DQN PAAEFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNSPAAEFPRD 65

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           FL ISLPEQPNKYYLIIRGQRIVLEADFTIQ IME LQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP +SENLRGIVME+EYLPISSMEKSRQ+M  FF+VWQEAMSKSSLPGRFMHIE 
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIES 185

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQSVRN 222
           NFAEYGL D+YTPQHTAVQY NVMAQLI TVQAVQS RN
Sbjct: 186 NFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023668 vs. ExPASy TrEMBL
Match: A0A0A0LHQ1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=3659 GN=MED20 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 7.5e-105
Identity = 198/219 (90.41%), Postives = 202/219 (92.24%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           VLHWQPNA STVNSQILNEVSQCVESIHGVKGGKWK T TFY+PILR DQN PAAEFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DQNSPAAEFPRD 65

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           FL ISLPEQPNKYYLIIRGQRIVLEADFTIQ IME LQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP +SENLRGIVME+EYLPISSMEKSRQVM  FF+VWQEAMSKSSLPGRFMHIE 
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFMHIES 185

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQSVRN 222
           NFAEYGL DYYT QHTAVQY NVMAQLI TVQAVQS RN
Sbjct: 186 NFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023668 vs. ExPASy TrEMBL
Match: A0A6J1KEU1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=3661 GN=LOC111493666 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 2.9e-104
Identity = 196/219 (89.50%), Postives = 202/219 (92.24%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           VLHWQPNA STVNSQILNEVSQCVESIHGVKGGKWK T TFY+PILR D N+ A EFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DTNIAAGEFPRD 65

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           FL IS+PEQPNKYYLIIRGQRIVLEADFTIQ IME LQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP +SENLRGIVME+EYLPISSMEKS+QVM  FFEVWQEAMSKSSLPGRFMHIEP
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEP 185

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQSVRN 222
           NFAEYGL DYYT QHTAVQYANVMAQLI TVQAVQS RN
Sbjct: 186 NFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023668 vs. ExPASy TrEMBL
Match: A0A6J1G4U3 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=3662 GN=LOC111450720 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 2.9e-104
Identity = 196/219 (89.50%), Postives = 202/219 (92.24%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           VLHWQPNA STVNSQILNEVSQCVESIHGVKGGKWK T TFY+PILR D N+ A EFPRD
Sbjct: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILR-DTNIAAGEFPRD 65

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           FL IS+PEQPNKYYLIIRGQRIVLEADFTIQ IME LQSYKLRVALNFEGFQYQLGDFQL
Sbjct: 66  FLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQL 125

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP +SENLRGIVME+EYLPISSMEKS+QVM  FFEVWQEAMSKSSLPGRFMHIEP
Sbjct: 126 RVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEP 185

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQSVRN 222
           NFAEYGL DYYT QHTAVQYANVMAQLI TVQAVQS RN
Sbjct: 186 NFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of MS023668 vs. TAIR 10
Match: AT2G28230.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 301.2 bits (770), Expect = 6.8e-82
Identity = 150/215 (69.77%), Postives = 179/215 (83.26%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           VLHWQPN  STV+SQILNE +QCVESI+GVK G+WK T  +Y+P+L+   N    EFPRD
Sbjct: 6   VLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKDQAN--QLEFPRD 65

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           FL ISL +QPNKYY IIR QRIVLEAD +IQ IME LQSYK +VAL F+GFQYQLGDF+L
Sbjct: 66  FLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLGDFRL 125

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP HSEN+RGIVME+EYLPISSMEK+++VM  F E+W EA++K SLPG+F++I+ 
Sbjct: 126 RVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFVNIDL 185

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQAVQ 218
           NF E+GL D YTPQHTAV+YA VMA +I TVQAV+
Sbjct: 186 NFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

[Close]

BLAST of MS023668 vs. TAIR 10
Match: AT4G09070.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 270.8 bits (691), Expect = 9.8e-73
Identity = 131/213 (61.50%), Postives = 170/213 (79.81%), Query Frame = 0


Query: 4   VLHWQPNASSTVNSQILNEVSQCVESIHGVKGGKWKPT-TFYRPILRADQNLPAAEFPRD 63
           +LHWQPN  ST +SQILNEV+Q +ES++GVK G WK T  +Y+P+L+   N   AEFPR+
Sbjct: 6   LLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQAN--QAEFPRE 65

Query: 64  FLRISLPEQPNKYYLIIRGQRIVLEADFTIQTIMENLQSYKLRVALNFEGFQYQLGDFQL 123
           F+ ISLPE+P+KYY +IR QRIV+EAD +IQ IME+LQSYK +++  FEG +YQLGDF+L
Sbjct: 66  FVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQLGDFRL 125

Query: 124 RVGKVVPKHSENLRGIVMEMEYLPISSMEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEP 183
           RVGKVVP H+E +RG+VME+EYLPISSM  ++++M  F E+WQEAMSK SLPG+F++ E 
Sbjct: 126 RVGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFVNKEL 185

Query: 184 NFAEYGLLDYYTPQHTAVQYANVMAQLIGTVQA 216
           NF ++GL D YTPQHTAV YA  MA L+  +QA
Sbjct: 186 NFEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

[Close]

BLAST of MS023668 vs. TAIR 10
Match: AT2G28020.1 (BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of subunit 20 of Mediator complex (TAIR:AT2G28230.1); Has 37 Blast hits to 37 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 37; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 80.9 bits (198), Expect = 1.4e-15
Identity = 41/68 (60.29%), Postives = 53/68 (77.94%), Query Frame = 0


Query: 150 MEKSRQVMGGFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLLDYYTPQHTAVQ-YANVMAQ 209
           ME +R+VM  F E+W EA++K SLPG+F++ + NF E+GL D YTPQHTAV+ YA VMA 
Sbjct: 1   MEIARKVMEEFLEIWNEALAKRSLPGKFVNKDLNFGEFGLGDNYTPQHTAVRNYALVMAH 60

Query: 210 LIGTVQAV 217
           +I TVQAV
Sbjct: 61  MIATVQAV 68

The following BLAST results are available for this feature:

BLAST of MS023668 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889134.1	1.7e-106	91.78	mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Beninca...	[more]
XP_008459711.1	5.4e-105	89.95	PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform ...	[more]
KAA0061469.1	5.4e-105	89.95	mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis...	[more]
XP_004141069.1	1.6e-104	90.41	mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60...	[more]
XP_022946745.1	5.9e-104	89.50	mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata...	[more]

BLAST of MS023668 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NPF4	9.5e-81	69.77	Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana ...	[more]
Q9M0S0	1.4e-71	61.50	Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana ...	[more]
Q54FL3	3.5e-06	28.48	Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium...	[more]

BLAST of MS023668 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CH02	2.6e-105	89.95	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. maku...	[more]
A0A1S3CAW6	2.6e-105	89.95	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 G...	[more]
A0A0A0LHQ1	7.5e-105	90.41	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=365...	[more]
A0A6J1KEU1	2.9e-104	89.50	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=36...	[more]
A0A6J1G4U3	2.9e-104	89.50	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=...	[more]

BLAST of MS023668 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28230.1	6.8e-82	69.77	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT4G09070.1	9.8e-73	61.50	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT2G28020.1	1.4e-15	60.29	BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013921	Mediator complex, subunit Med20	PFAM	PF08612	Med20	coord: 4..206 e-value: 1.2E-35 score: 122.9
IPR013921	Mediator complex, subunit Med20	PANTHER	PTHR12465	UBIQUITIN SPECIFIC PROTEASE HOMOLOG 49	coord: 4..215
None	No IPR available	PANTHER	PTHR12465:SF0	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 20	coord: 4..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS023668.1	MS023668.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003712	transcription coregulator activity